MD10G1262900.v1.1

prefoldin subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011410
Physical Location & Seq
Reverse (-)
35735245 .. 35737934
2690 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1262900.v1.1.491

Sequence Viewer

Length: 393 bp
ATGGCGTCGTCGTCAGCTATTCGGGAGCTTCAGCGCGAATTGGAGGCCAAAGCTAACGATCTCAGCAAAATCCAAAAAGATATTTCAAAGAACCACCAAGTGAGGAAGAAGTACACTATACAGCTCGGCGAGAACGAGCTCGTCCTCAAGGAATTGGATCTCCTCAGCGAAGACGCCAATGTTTTCAAGTTGATAGGTCCGGTTCTTGTGAAGCAGGATTTGGCAGAGGCTAGGGCCAATGTGCGAAAGAGAATCGAATACATCTCAGCGGAACTGAAACGGCTGGATGCTACTCTTCAAGATTTGGAAGAGAAGCAAAATAGCAAGAAGGAAACGATTTTTAAATTACAGCAGAGGGCTCAAGCACTCCAGGCTGGAAAAGCAAAGGCATAA

Protein Analysis

131

Amino Acids

14.78

Weight (kDa)

9.42

Isoelectric Point (pI)

39.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prefoldin_2 PF01920 16 - 119 7.4e-23 Prefoldin subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 36
AciI CCGC 1 cut(s) 269
AclWI GGATC 1 cut(s) 165
AcuI CTGAAG 1 cut(s) 14
AcyI GRCGYC 2 cut(s) 5, 174
AdeI CACNNNGTG 1 cut(s) 100
AfaI GTAC 1 cut(s) 113
AgsI TTSAA 3 cut(s) 87, 187, 299
AjnI CCWGG 1 cut(s) 369
AjuI GAANNNNNNNTTGG 2 cut(s) 203, 235
AluBI AGCT 5 cut(s) 17, 28, 53, 124, 139
AluI AGCT 5 cut(s) 17, 28, 53, 124, 139
Alw21I GWGCWC 1 cut(s) 141
AlwI GGATC 1 cut(s) 165
AoxI GGCC 2 cut(s) 45, 234
AspLEI GCGC 1 cut(s) 36
AspS9I GGNCC 2 cut(s) 197, 234
AvaII GGWCC 1 cut(s) 197
BanII GRGCYC 2 cut(s) 141, 361
BbsI GAAGAC 1 cut(s) 177
Bbv12I GWGCWC 1 cut(s) 141
BbvCI CCTCAGC 1 cut(s) 164
BceAI ACGGC 1 cut(s) 296
BciT130I CCWGG 1 cut(s) 371
BfaI CTAG 1 cut(s) 231
Bme1390I CCNGG 1 cut(s) 371
Bme18I GGWCC 1 cut(s) 197
BmgT120I GGNCC 2 cut(s) 197, 234
BmrFI CCNGG 1 cut(s) 371
BmsI GCATC 1 cut(s) 277
BpiI GAAGAC 1 cut(s) 177
BpmI CTGGAG 1 cut(s) 353
Bpu10I CCTNAGC 1 cut(s) 164
BpuEI CTTGAG 2 cut(s) 131, 345
BsaHI GRCGYC 2 cut(s) 5, 174
BsaWI WCCGGW 1 cut(s) 199
BseBI CCWGG 1 cut(s) 371
BseGI GGATG 1 cut(s) 292
BseMII CTCAG 3 cut(s) 76, 178, 279
BseRI GAGGAG 1 cut(s) 152
Bsh1236I CGCG 1 cut(s) 36
BshFI GGCC 2 cut(s) 47, 236
BsiHKAI GWGCWC 1 cut(s) 141
BsiSI CCGG 1 cut(s) 200
BsnI GGCC 2 cut(s) 47, 236
Bsp1286I GDGCHC 2 cut(s) 141, 361
Bsp143I GATC 2 cut(s) 58, 157
BspACI CCGC 1 cut(s) 269
BspANI GGCC 2 cut(s) 47, 236
BspCNI CTCAG 3 cut(s) 75, 177, 278
BspFNI CGCG 1 cut(s) 36
BspPI GGATC 1 cut(s) 165
BssMI GATC 2 cut(s) 58, 157
BssNI GRCGYC 2 cut(s) 5, 174
Bst2UI CCWGG 1 cut(s) 371
Bst6I CTCTTC 2 cut(s) 300, 303
BstACI GRCGYC 2 cut(s) 5, 174
BstDEI CTNAG 3 cut(s) 62, 164, 265
BstF5I GGATG 1 cut(s) 292
BstFNI CGCG 1 cut(s) 36
BstHHI GCGC 1 cut(s) 36
BstKTI GATC 2 cut(s) 61, 160
BstMBI GATC 2 cut(s) 58, 157
BstMWI GCNNNNNNNGC 2 cut(s) 371, 380
BstNI CCWGG 1 cut(s) 371
BstSCI CCNGG 1 cut(s) 369
BstUI CGCG 1 cut(s) 36
BstV2I GAAGAC 1 cut(s) 177
BstX2I RGATCY 1 cut(s) 157
BstYI RGATCY 1 cut(s) 157
BsuRI GGCC 2 cut(s) 47, 236
BtsCI GGATG 1 cut(s) 292
CfoI GCGC 1 cut(s) 36
Cfr13I GGNCC 2 cut(s) 197, 234
CseI GACGC 1 cut(s) 182
Csp6I GTAC 1 cut(s) 112
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 3 cut(s) 62, 164, 265
DpnI GATC 2 cut(s) 60, 159
DpnII GATC 2 cut(s) 58, 157
DraI TTTAAA 1 cut(s) 343
DraIII CACNNNGTG 1 cut(s) 100
Eam1104I CTCTTC 2 cut(s) 300, 303
EarI CTCTTC 2 cut(s) 300, 303
Ecl136II GAGCTC 1 cut(s) 139
Eco24I GRGCYC 2 cut(s) 141, 361
Eco47I GGWCC 1 cut(s) 197
Eco53kI GAGCTC 1 cut(s) 139
Eco57I CTGAAG 1 cut(s) 14
EcoICRI GAGCTC 1 cut(s) 139
EcoRII CCWGG 1 cut(s) 369
EcoT38I GRGCYC 2 cut(s) 141, 361
FaiI YATR 2 cut(s) 119, 391
FokI GGATG 1 cut(s) 299
FriOI GRGCYC 2 cut(s) 141, 361
FspBI CTAG 1 cut(s) 231
GlaI GCGC 1 cut(s) 35
GsuI CTGGAG 1 cut(s) 353
HaeIII GGCC 2 cut(s) 47, 236
HapII CCGG 1 cut(s) 200
HgaI GACGC 1 cut(s) 182
HhaI GCGC 1 cut(s) 36
Hin1I GRCGYC 2 cut(s) 5, 174
Hin6I GCGC 1 cut(s) 34
HinP1I GCGC 1 cut(s) 34
HinfI GANTC 1 cut(s) 252
HpaII CCGG 1 cut(s) 200
Hpy166II GTNNAC 1 cut(s) 114
Hpy188III TCNNGA 2 cut(s) 23, 299
Hpy8I GTNNAC 1 cut(s) 114
Hpy99I CGWCG 2 cut(s) 10, 13
HpyAV CCTTC 1 cut(s) 322
HpyF10VI GCNNNNNNNGC 2 cut(s) 371, 380
HpyF3I CTNAG 3 cut(s) 62, 164, 265
Hsp92I GRCGYC 2 cut(s) 5, 174
HspAI GCGC 1 cut(s) 34
Kzo9I GATC 2 cut(s) 58, 157
LmnI GCTCC 1 cut(s) 25
LpnPI CCDG 6 cut(s) 200, 213, 269, 356, 360, 383
LweI GCATC 1 cut(s) 277
MaeI CTAG 1 cut(s) 231
MalI GATC 2 cut(s) 60, 159
MboI GATC 2 cut(s) 58, 157
MboII GAAGA 4 cut(s) 118, 182, 287, 320
MflI RGATCY 1 cut(s) 157
MhlI GDGCHC 2 cut(s) 141, 361
MluCI AATT 3 cut(s) 38, 152, 344
MnlI CCTC 6 cut(s) 37, 96, 155, 173, 220, 348
MseI TTAA 1 cut(s) 342
MspA1I CMGCKG 1 cut(s) 269
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 1 cut(s) 371
MvaI CCWGG 1 cut(s) 371
MvnI CGCG 1 cut(s) 36
MwoI GCNNNNNNNGC 2 cut(s) 371, 380
NdeII GATC 2 cut(s) 58, 157
NmeAIII GCCGAG 1 cut(s) 105
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PfeI GAWTC 1 cut(s) 252
Psp124BI GAGCTC 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 369
PspGI CCWGG 1 cut(s) 369
PspPI GGNCC 2 cut(s) 197, 234
PsuI RGATCY 1 cut(s) 157
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
SacI GAGCTC 1 cut(s) 141
SaqAI TTAA 1 cut(s) 342
Sau3AI GATC 2 cut(s) 58, 157
Sau96I GGNCC 2 cut(s) 197, 234
ScrFI CCNGG 1 cut(s) 371
SduI GDGCHC 2 cut(s) 141, 361
SetI ASST 6 cut(s) 19, 30, 55, 126, 141, 199
SfaNI GCATC 1 cut(s) 277
SinI GGWCC 1 cut(s) 197
SmlI CTYRAG 2 cut(s) 146, 360
SmoI CTYRAG 2 cut(s) 146, 360
Sse9I AATT 3 cut(s) 38, 152, 344
SsiI CCGC 1 cut(s) 269
SspMI CTAG 1 cut(s) 231
SstI GAGCTC 1 cut(s) 141
StyD4I CCNGG 1 cut(s) 369
TaqI TCGA 1 cut(s) 255
TasI AATT 3 cut(s) 38, 152, 344
TatI WGTACW 1 cut(s) 111
TfiI GAWTC 1 cut(s) 252
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
VpaK11BI GGWCC 1 cut(s) 197
XspI CTAG 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.