MD05G1285700.v1.1

urease accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012327
Physical Location & Seq
Forward (+)
41918299 .. 41920725
2427 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1285700.v1.1.491

Sequence Viewer

Length: 828 bp
ATGGCTGACCACCACCATGACCATCACCACCATCATGATCATGATCATGGTCATAGCCATTTGAGTTCAGCAACACCAAATTCATTTGTGGGCCCAGATGGGAAGGTGTACCATAGCCACGATGGGCTGGCACCACACTCACACGAACCCATATACTCCCCCGGGTACTTCAGCAGAAGAGCTCCACCTCTCCTCTCCAGGAATTTCAATGAAAGGGCTTTCACCATTGGCATTGGTGGACCTGTTGGTACCGGGAAAACTGCTCTGATGTTGGCTCTGTGTAAACTTTTGCGTGACAAGTACAGTCTTGCTGCTGTGACAAATGATATATTCACAAAAGAGGATGGTGAGTTCTTGGTGAAGAATGGAGCACTTCCGGAAGAAAGAATACGTGCTGTGGAAACTGGGGGCTGCCCACATGCTGCAATTCGCGAAGACATCAGTATTAACCTTGGCCCTCTTGAGGAGCTTTCAAACTTGTACAAGACCGACATACTGCTTTGTGAATCTGGCGGAGATAATTTAGCCGCGAACTTCAGCAGGGAGCTGGCTGACTATATCATTTACATAATCGATGTATCTGGAGGTGATAAAATTCCACGAAAAGGTGGCCCCGGCATCACTCAAGCTGACCTCCTGGTGATAAACAAGACTGACCTTGCACCAGCAGTTGGAGCTGATTTGGCAGTGATGGAGCGTGATGCACTACGAATGCGAGATGGAGGGCCATTTGTCTTTGCTCAGGTGAAGCACGGGAAAGGAGTAGAGGAAATCGTGAACCACATATTACAGGCTTGGGAAGTAGCAACAGGGAAGAAGCGCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006807 GO:0008150 GO:0008152 GO:0043085 GO:0044093 GO:0050790 GO:0065007 GO:0065009
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cobW

Protein Analysis

276

Amino Acids

29.95

Weight (kDa)

6.18

Isoelectric Point (pI)

28.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 75 - 245 7e-36 CobW/HypB/UreG, nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012327)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34470 AT2G34470
fragaria_vesca FvH4_3g05780
malus_domestica MD05G1285700.v1.1 MD10G1263700.v1.1
prunus_persica Prupe.4G079000_v2.0.a1
pyrus_communis pycom10g21990
rosa_chinensis RchiOBHm_Chr5g0013721 RchiOBHm_Chr5g0013731 RchiOBHm_Chr5g0013741 RchiOBHm_Chr5g0013751
rosa_laevigata RLG00000032043
rosa_multiflora Rmu_ssc0000256.1_g000001
rosa_roxburghii Rroxscaffold_1G00062890
rosa_rugosa Rorug05G0012600
rosa_samantha Rh5AG106900 Rh5BG103000 Rh5DG101700
rosa_wichuraiana Rw5G009320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 248
AccB1I GGYRCC 2 cut(s) 130, 248
AccB7I CCANNNNNTGG 1 cut(s) 671
AccII CGCG 2 cut(s) 432, 530
AccIII TCCGGA 1 cut(s) 376
AciI CCGC 2 cut(s) 513, 528
AcsI RAATTY 3 cut(s) 79, 202, 594
AcuI CTGAAG 2 cut(s) 154, 520
AfaI GTAC 5 cut(s) 110, 167, 250, 302, 482
AfiI CCNNNNNNNGG 3 cut(s) 463, 605, 671
AgsI TTSAA 2 cut(s) 208, 474
AjnI CCWGG 2 cut(s) 197, 636
AloI GAACNNNNNNTCC 2 cut(s) 335, 367
AluBI AGCT 5 cut(s) 182, 469, 547, 629, 677
AluI AGCT 5 cut(s) 182, 469, 547, 629, 677
Alw21I GWGCWC 2 cut(s) 184, 373
Ama87I CYCGRG 1 cut(s) 161
Aor13HI TCCGGA 1 cut(s) 376
AoxI GGCC 4 cut(s) 91, 454, 610, 725
ApaI GGGCCC 1 cut(s) 95
ApeKI GCWGC 3 cut(s) 311, 411, 422
ApoI RAATTY 3 cut(s) 79, 202, 594
Asp718I GGTACC 1 cut(s) 248
AspLEI GCGC 1 cut(s) 822
AspS9I GGNCC 6 cut(s) 91, 92, 239, 455, 611, 725
AsuC2I CCSGG 4 cut(s) 162, 163, 253, 615
AsuHPI GGTGA 7 cut(s) 17, 214, 359, 370, 599, 652, 757
AvaI CYCGRG 1 cut(s) 161
AvaII GGWCC 1 cut(s) 239
BaeGI GKGCMC 1 cut(s) 95
BanI GGYRCC 2 cut(s) 130, 248
BanII GRGCYC 2 cut(s) 95, 184
BarI GAAGNNNNNNTAC 2 cut(s) 372, 404
BbsI GAAGAC 1 cut(s) 441
Bbv12I GWGCWC 2 cut(s) 184, 373
BbvI GCAGC 3 cut(s) 298, 398, 409
BccI CCATC 7 cut(s) 30, 39, 92, 116, 338, 685, 713
BciT130I CCWGG 2 cut(s) 199, 638
BclI TGATCA 2 cut(s) 37, 43
BcnI CCSGG 4 cut(s) 162, 163, 253, 615
BfoI RGCGCY 1 cut(s) 823
BisI GCNGC 4 cut(s) 312, 412, 423, 528
BlsI GCNGC 4 cut(s) 313, 413, 424, 529
Bme1390I CCNGG 6 cut(s) 162, 163, 199, 253, 615, 638
Bme18I GGWCC 1 cut(s) 239
BmeT110I CYCGRG 1 cut(s) 161
BmgT120I GGNCC 6 cut(s) 91, 92, 239, 455, 611, 725
BmiI GGNNCC 4 cut(s) 93, 132, 250, 613
BmrFI CCNGG 6 cut(s) 162, 163, 199, 253, 615, 638
BmrI ACTGGG 1 cut(s) 414
BmsI GCATC 2 cut(s) 627, 691
BmuI ACTGGG 1 cut(s) 414
BpiI GAAGAC 1 cut(s) 441
BpmI CTGGAG 2 cut(s) 181, 603
Bpu10I CCTNAGC 1 cut(s) 741
BpuEI CTTGAG 2 cut(s) 482, 609
BpuMI CCSGG 4 cut(s) 162, 163, 253, 615
Bsa29I ATCGAT 1 cut(s) 573
BsaAI YACGTR 1 cut(s) 392
BsaBI GATNNNNATC 1 cut(s) 42
BsaJI CCNNGG 4 cut(s) 160, 161, 451, 613
BsaWI WCCGGW 1 cut(s) 376
Bsc4I CCNNNNNNNGG 3 cut(s) 463, 605, 671
Bse1I ACTGG 1 cut(s) 409
Bse8I GATNNNNATC 1 cut(s) 42
BseAI TCCGGA 1 cut(s) 376
BseBI CCWGG 2 cut(s) 199, 638
BseCI ATCGAT 1 cut(s) 573
BseDI CCNNGG 4 cut(s) 160, 161, 451, 613
BseGI GGATG 1 cut(s) 349
BseJI GATNNNNATC 1 cut(s) 42
BseLI CCNNNNNNNGG 3 cut(s) 463, 605, 671
BseMII CTCAG 1 cut(s) 755
BseNI ACTGG 1 cut(s) 409
BseRI GAGGAG 2 cut(s) 182, 479
BseSI GKGCMC 1 cut(s) 95
BseXI GCAGC 3 cut(s) 298, 398, 409
Bsh1236I CGCG 2 cut(s) 432, 530
BshFI GGCC 4 cut(s) 93, 456, 612, 727
BshNI GGYRCC 2 cut(s) 130, 248
BshVI ATCGAT 1 cut(s) 573
BsiHKAI GWGCWC 2 cut(s) 184, 373
BsiHKCI CYCGRG 1 cut(s) 161
BsiSI CCGG 4 cut(s) 162, 252, 377, 615
BslI CCNNNNNNNGG 3 cut(s) 463, 605, 671
BsmI GAATGC 1 cut(s) 717
BsnI GGCC 4 cut(s) 93, 456, 612, 727
BsoBI CYCGRG 1 cut(s) 161
Bsp120I GGGCCC 1 cut(s) 91
Bsp1286I GDGCHC 3 cut(s) 95, 184, 373
Bsp13I TCCGGA 1 cut(s) 376
Bsp1407I TGTACA 1 cut(s) 480
Bsp143I GATC 2 cut(s) 37, 43
Bsp68I TCGCGA 1 cut(s) 432
BspACI CCGC 2 cut(s) 513, 528
BspANI GGCC 4 cut(s) 93, 456, 612, 727
BspCNI CTCAG 1 cut(s) 754
BspDI ATCGAT 1 cut(s) 573
BspEI TCCGGA 1 cut(s) 376
BspFNI CGCG 2 cut(s) 432, 530
BspHI TCATGA 2 cut(s) 34, 40
BspLI GGNNCC 4 cut(s) 93, 132, 250, 613
BspQI GCTCTTC 1 cut(s) 172
BspT107I GGYRCC 2 cut(s) 130, 248
BsrGI TGTACA 1 cut(s) 480
BsrI ACTGG 1 cut(s) 409
BssECI CCNNGG 4 cut(s) 160, 161, 451, 613
BssMI GATC 2 cut(s) 37, 43
BssT1I CCWWGG 1 cut(s) 451
Bst2UI CCWGG 2 cut(s) 199, 638
Bst4CI ACNGT 1 cut(s) 305
Bst6I CTCTTC 1 cut(s) 172
BstAUI TGTACA 1 cut(s) 480
BstBAI YACGTR 1 cut(s) 392
BstC8I GCNNGC 2 cut(s) 129, 549
BstDEI CTNAG 1 cut(s) 741
BstF5I GGATG 1 cut(s) 349
BstFNI CGCG 2 cut(s) 432, 530
BstH2I RGCGCY 1 cut(s) 823
BstHHI GCGC 1 cut(s) 822
BstKTI GATC 2 cut(s) 40, 46
BstMBI GATC 2 cut(s) 37, 43
BstMWI GCNNNNNNNGC 2 cut(s) 674, 683
BstNI CCWGG 2 cut(s) 199, 638
BstNSI RCATGY 1 cut(s) 422
BstSCI CCNGG 6 cut(s) 160, 161, 197, 251, 613, 636
BstSLI GKGCMC 1 cut(s) 95
BstUI CGCG 2 cut(s) 432, 530
BstV1I GCAGC 3 cut(s) 298, 398, 409
BstV2I GAAGAC 1 cut(s) 441
Bsu15I ATCGAT 1 cut(s) 573
BsuRI GGCC 4 cut(s) 93, 456, 612, 727
BsuTUI ATCGAT 1 cut(s) 573
BtsCI GGATG 1 cut(s) 349
BtsI GCAGTG 1 cut(s) 693
BtsIMutI CAGTG 2 cut(s) 693, 823
BtuMI TCGCGA 1 cut(s) 432
Cac8I GCNNGC 2 cut(s) 129, 549
CciI TCATGA 2 cut(s) 34, 40
CfoI GCGC 1 cut(s) 822
Cfr13I GGNCC 6 cut(s) 91, 92, 239, 455, 611, 725
Cfr9I CCCGGG 1 cut(s) 161
ClaI ATCGAT 1 cut(s) 573
Csp6I GTAC 5 cut(s) 109, 166, 249, 301, 481
CviAII CATG 5 cut(s) 17, 35, 41, 47, 419
CviQI GTAC 5 cut(s) 109, 166, 249, 301, 481
DdeI CTNAG 1 cut(s) 741
DpnI GATC 2 cut(s) 39, 45
DpnII GATC 2 cut(s) 37, 43
Eam1104I CTCTTC 1 cut(s) 172
EarI CTCTTC 1 cut(s) 172
EciI GGCGGA 1 cut(s) 528
Ecl136II GAGCTC 1 cut(s) 182
Eco130I CCWWGG 1 cut(s) 451
Eco24I GRGCYC 2 cut(s) 95, 184
Eco47I GGWCC 1 cut(s) 239
Eco53kI GAGCTC 1 cut(s) 182
Eco57I CTGAAG 2 cut(s) 154, 520
Eco88I CYCGRG 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 182
EcoRII CCWGG 2 cut(s) 197, 636
EcoT14I CCWWGG 1 cut(s) 451
EcoT38I GRGCYC 2 cut(s) 95, 184
ErhI CCWWGG 1 cut(s) 451
FaeI CATG 5 cut(s) 20, 38, 44, 50, 422
FatI CATG 5 cut(s) 16, 34, 40, 46, 418
FbaI TGATCA 2 cut(s) 37, 43
Fnu4HI GCNGC 4 cut(s) 312, 412, 423, 528
FokI GGATG 1 cut(s) 356
FriOI GRGCYC 2 cut(s) 95, 184
Fsp4HI GCNGC 4 cut(s) 312, 412, 423, 528
GlaI GCGC 1 cut(s) 821
GluI GCNGC 4 cut(s) 312, 412, 423, 528
GsuI CTGGAG 2 cut(s) 181, 603
HaeII RGCGCY 1 cut(s) 823
HaeIII GGCC 4 cut(s) 93, 456, 612, 727
HapII CCGG 4 cut(s) 162, 252, 377, 615
HhaI GCGC 1 cut(s) 822
Hin1II CATG 5 cut(s) 20, 38, 44, 50, 422
Hin6I GCGC 1 cut(s) 820
HinP1I GCGC 1 cut(s) 820
HinfI GANTC 1 cut(s) 506
HpaII CCGG 4 cut(s) 162, 252, 377, 615
HphI GGTGA 7 cut(s) 17, 214, 359, 370, 599, 652, 757
Hpy166II GTNNAC 4 cut(s) 109, 239, 284, 778
Hpy188I TCNGA 1 cut(s) 267
Hpy188III TCNNGA 7 cut(s) 35, 41, 377, 431, 461, 582, 775
Hpy8I GTNNAC 4 cut(s) 109, 239, 284, 778
HpyAV CCTTC 1 cut(s) 97
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4IV ACGT 1 cut(s) 391
HpyCH4V TGCA 3 cut(s) 425, 662, 704
HpyF10VI GCNNNNNNNGC 2 cut(s) 674, 683
HpyF3I CTNAG 1 cut(s) 741
HpySE526I ACGT 1 cut(s) 391
Hsp92II CATG 5 cut(s) 20, 38, 44, 50, 422
HspAI GCGC 1 cut(s) 820
Kpn2I TCCGGA 1 cut(s) 376
KpnI GGTACC 1 cut(s) 252
Ksp22I TGATCA 2 cut(s) 37, 43
Kzo9I GATC 2 cut(s) 37, 43
LguI GCTCTTC 1 cut(s) 172
LmnI GCTCC 6 cut(s) 187, 368, 466, 544, 674, 694
Lsp1109I GCAGC 3 cut(s) 298, 398, 409
LweI GCATC 2 cut(s) 627, 691
MaeII ACGT 1 cut(s) 391
MaeIII GTNAC 2 cut(s) 293, 316
MalI GATC 2 cut(s) 39, 45
MboI GATC 2 cut(s) 37, 43
MboII GAAGA 5 cut(s) 189, 373, 392, 446, 826
MhlI GDGCHC 3 cut(s) 95, 184, 373
MluCI AATT 5 cut(s) 79, 202, 426, 520, 594
MmeI TCCRAC 1 cut(s) 652
MnlI CCTC 9 cut(s) 198, 203, 334, 457, 468, 578, 644, 716, 760
MroI TCCGGA 1 cut(s) 376
MseI TTAA 1 cut(s) 447
MslI CAYNNNNRTG 4 cut(s) 15, 33, 39, 45
MspI CCGG 4 cut(s) 162, 252, 377, 615
MspR9I CCNGG 6 cut(s) 162, 163, 199, 253, 615, 638
Mva1269I GAATGC 1 cut(s) 717
MvaI CCWGG 2 cut(s) 199, 638
MvnI CGCG 2 cut(s) 432, 530
MwoI GCNNNNNNNGC 2 cut(s) 674, 683
NciI CCSGG 4 cut(s) 162, 163, 253, 615
NdeII GATC 2 cut(s) 37, 43
NlaIII CATG 5 cut(s) 20, 38, 44, 50, 422
NlaIV GGNNCC 4 cut(s) 93, 132, 250, 613
NmuCI GTSAC 2 cut(s) 293, 316
NruI TCGCGA 1 cut(s) 432
NspI RCATGY 1 cut(s) 422
PagI TCATGA 2 cut(s) 34, 40
PciSI GCTCTTC 1 cut(s) 172
PctI GAATGC 1 cut(s) 717
PfeI GAWTC 1 cut(s) 506
PflMI CCANNNNNTGG 1 cut(s) 671
PfoI TCCNGGA 1 cut(s) 197
PkrI GCNGC 4 cut(s) 313, 413, 424, 529
Ppu21I YACGTR 1 cut(s) 392
Psp124BI GAGCTC 1 cut(s) 184
Psp6I CCWGG 2 cut(s) 197, 636
PspGI CCWGG 2 cut(s) 197, 636
PspN4I GGNNCC 4 cut(s) 93, 132, 250, 613
PspOMI GGGCCC 1 cut(s) 91
PspPI GGNCC 6 cut(s) 91, 92, 239, 455, 611, 725
RruI TCGCGA 1 cut(s) 432
RsaI GTAC 5 cut(s) 110, 167, 250, 302, 482
RsaNI GTAC 5 cut(s) 109, 166, 249, 301, 481
RseI CAYNNNNRTG 4 cut(s) 15, 33, 39, 45
SacI GAGCTC 1 cut(s) 184
SapI GCTCTTC 1 cut(s) 172
SaqAI TTAA 1 cut(s) 447
SatI GCNGC 4 cut(s) 312, 412, 423, 528
Sau3AI GATC 2 cut(s) 37, 43
Sau96I GGNCC 6 cut(s) 91, 92, 239, 455, 611, 725
ScrFI CCNGG 6 cut(s) 162, 163, 199, 253, 615, 638
SduI GDGCHC 3 cut(s) 95, 184, 373
SfaNI GCATC 2 cut(s) 627, 691
SinI GGWCC 1 cut(s) 239
SmaI CCCGGG 1 cut(s) 163
SmiMI CAYNNNNRTG 4 cut(s) 15, 33, 39, 45
SmlI CTYRAG 2 cut(s) 461, 624
SmoI CTYRAG 2 cut(s) 461, 624
Sse9I AATT 5 cut(s) 79, 202, 426, 520, 594
SsiI CCGC 2 cut(s) 513, 528
SstI GAGCTC 1 cut(s) 184
StyD4I CCNGG 6 cut(s) 160, 161, 197, 251, 613, 636
StyI CCWWGG 1 cut(s) 451
TaaI ACNGT 1 cut(s) 305
TaiI ACGT 1 cut(s) 394
TaqI TCGA 1 cut(s) 573
TaqII GACCGA 1 cut(s) 503
TasI AATT 5 cut(s) 79, 202, 426, 520, 594
TatI WGTACW 2 cut(s) 300, 480
TauI GCSGC 1 cut(s) 530
TfiI GAWTC 1 cut(s) 506
Tru1I TTAA 1 cut(s) 447
Tru9I TTAA 1 cut(s) 447
TscAI CASTG 1 cut(s) 693
TseFI GTSAC 2 cut(s) 293, 316
TseI GCWGC 3 cut(s) 311, 411, 422
Tsp45I GTSAC 2 cut(s) 293, 316
TspDTI ATGAA 2 cut(s) 72, 225
TspMI CCCGGG 1 cut(s) 161
TspRI CASTG 1 cut(s) 693
Van91I CCANNNNNTGG 1 cut(s) 671
VpaK11BI GGWCC 1 cut(s) 239
XapI RAATTY 3 cut(s) 79, 202, 594
XceI RCATGY 1 cut(s) 422
XcmI CCANNNNNNNNNTGG 1 cut(s) 119
XmaI CCCGGG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.