pycom10g21990

urease accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0012327
Physical Location & Seq
Forward (+)
24520564 .. 24523201
2638 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g21990.3

Sequence Viewer

Length: 876 bp
ATGTACCCACCCAAACCAACCAGAATCCCCCGATGGCTGACCACCACCATGATCATCATGACCATGACCATCATCACCACCATGATCATGATCATCGATCATAGCCATGGGAGTTCAGCTGCAACGAATTCATGGGTGGGCGCAGATGGGAAGGTGTACCATAGCCACGATGGGCTGGCGCCACACTCGCACGAACCCATATACTCCCCTGGCTACTTCAGCAGAAGAGCTCCACCTCTCGTCTCCAGGAATTTCAATGAAAGGGCTTTCACCATTGGCATTGGTGGACCTGTTGGTACTGGGAAAACTGCTTTGATGCTCGCTCTGTGTACACTTTTGCGTGACAAGTACAGTCTTGCTGCAGTGACAAATGATATATTCACAAAAGAGGATGGTGAGTTCTTGGTGAAGCATGGAGCACTTCCCGAAGAAAGAATACGTGCTGTGGAAACTGGGGGCTGCCCACATGCTGCAATTCGTGAAGACATCAGTATTAACCTTGGCCCTCTTGAGGAGCTTTCTAACTTGTACAAGACCGACATACTGCTTTGTGAATCAGGCGGAGATAATTTAGCTGCAAACTTCAGCAGAGAACTGGCCGACTATATTATTTACATTATAGATGTCTCTGCTGGTGATAAAATTCCACGAAAAGGTGGCCCTGGAATCACTCAGGCTGACCTTCTGGTGATAAACAAGACTGATCTTGCAGCAGCAGTTGGAGCTGATTTGGCAGTGATGGAGCGTGATGCACTTCGAATGCGAGATGGAGGGCCTTTTGTCTTTGCTCAGGTGAAGCACGGGAAAGGAGTTGAAGAAATCGTGAACCACATATTACAGGCTTGGGAGGTAGCAACAGGGAAGAAGCGCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006807 GO:0008150 GO:0008152 GO:0043085 GO:0044093 GO:0050790 GO:0065007 GO:0065009
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cobW

Protein Analysis

292

Amino Acids

31.67

Weight (kDa)

6.41

Isoelectric Point (pI)

29.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012327)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34470 AT2G34470
fragaria_vesca FvH4_3g05780
malus_domestica MD05G1285700.v1.1 MD10G1263700.v1.1
prunus_persica Prupe.4G079000_v2.0.a1
pyrus_communis pycom10g21990
rosa_chinensis RchiOBHm_Chr5g0013721 RchiOBHm_Chr5g0013731 RchiOBHm_Chr5g0013741 RchiOBHm_Chr5g0013751
rosa_laevigata RLG00000032043
rosa_multiflora Rmu_ssc0000256.1_g000001
rosa_roxburghii Rroxscaffold_1G00062890
rosa_rugosa Rorug05G0012600
rosa_samantha Rh5AG106900 Rh5BG103000 Rh5DG101700
rosa_wichuraiana Rw5G009320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 178
AciI CCGC 1 cut(s) 561
AcoI YGGCCR 1 cut(s) 597
AcsI RAATTY 3 cut(s) 127, 250, 642
AcuI CTGAAG 2 cut(s) 202, 568
AcyI GRCGYC 1 cut(s) 179
AfaI GTAC 6 cut(s) 5, 158, 298, 331, 350, 530
AfiI CCNNNNNNNGG 2 cut(s) 511, 653
AgsI TTSAA 2 cut(s) 256, 815
AjnI CCWGG 3 cut(s) 208, 245, 661
AloI GAACNNNNNNTCC 2 cut(s) 383, 415
AluBI AGCT 5 cut(s) 119, 230, 517, 575, 725
AluI AGCT 5 cut(s) 119, 230, 517, 575, 725
Alw21I GWGCWC 2 cut(s) 232, 421
Alw26I GTCTC 2 cut(s) 247, 631
AoxI GGCC 4 cut(s) 502, 597, 658, 773
ApeKI GCWGC 7 cut(s) 119, 359, 459, 470, 575, 710, 713
ApoI RAATTY 3 cut(s) 127, 250, 642
AspLEI GCGC 3 cut(s) 143, 181, 870
AspS9I GGNCC 4 cut(s) 287, 503, 659, 773
AsuHPI GGTGA 7 cut(s) 67, 262, 407, 418, 647, 700, 805
AsuII TTCGAA 1 cut(s) 757
AvaII GGWCC 1 cut(s) 287
BanI GGYRCC 1 cut(s) 178
BanII GRGCYC 1 cut(s) 232
BarI GAAGNNNNNNTAC 2 cut(s) 420, 452
BbsI GAAGAC 1 cut(s) 489
Bbv12I GWGCWC 2 cut(s) 232, 421
BbvI GCAGC 7 cut(s) 106, 346, 446, 457, 562, 722, 725
BccI CCATC 7 cut(s) 27, 77, 140, 164, 386, 733, 761
BciT130I CCWGG 3 cut(s) 210, 247, 663
BclI TGATCA 3 cut(s) 51, 84, 90
BcoDI GTCTC 2 cut(s) 247, 631
BfmI CTRYAG 1 cut(s) 360
BfoI RGCGCY 2 cut(s) 182, 871
BisI GCNGC 7 cut(s) 120, 360, 460, 471, 576, 711, 714
BlsI GCNGC 7 cut(s) 121, 361, 461, 472, 577, 712, 715
Bme1390I CCNGG 3 cut(s) 210, 247, 663
Bme18I GGWCC 1 cut(s) 287
BmgT120I GGNCC 4 cut(s) 287, 503, 659, 773
BmiI GGNNCC 1 cut(s) 180
BmrFI CCNGG 3 cut(s) 210, 247, 663
BmrI ACTGGG 2 cut(s) 309, 462
BmsI GCATC 2 cut(s) 306, 739
BmuI ACTGGG 2 cut(s) 309, 462
BpiI GAAGAC 1 cut(s) 489
BpmI CTGGAG 1 cut(s) 229
Bpu10I CCTNAGC 1 cut(s) 789
Bpu14I TTCGAA 1 cut(s) 757
BpuEI CTTGAG 1 cut(s) 530
Bsa29I ATCGAT 1 cut(s) 96
BsaAI YACGTR 1 cut(s) 440
BsaBI GATNNNNATC 1 cut(s) 89
BsaHI GRCGYC 1 cut(s) 179
BsaJI CCNNGG 4 cut(s) 106, 208, 499, 661
Bsc4I CCNNNNNNNGG 2 cut(s) 511, 653
Bse1I ACTGG 3 cut(s) 304, 457, 600
Bse8I GATNNNNATC 1 cut(s) 89
BseBI CCWGG 3 cut(s) 210, 247, 663
BseCI ATCGAT 1 cut(s) 96
BseDI CCNNGG 4 cut(s) 106, 208, 499, 661
BseGI GGATG 1 cut(s) 397
BseJI GATNNNNATC 1 cut(s) 89
BseLI CCNNNNNNNGG 2 cut(s) 511, 653
BseMII CTCAG 2 cut(s) 686, 803
BseNI ACTGG 3 cut(s) 304, 457, 600
BseRI GAGGAG 1 cut(s) 527
BseXI GCAGC 7 cut(s) 106, 346, 446, 457, 562, 722, 725
BshFI GGCC 4 cut(s) 504, 599, 660, 775
BshNI GGYRCC 1 cut(s) 178
BshVI ATCGAT 1 cut(s) 96
BsiHKAI GWGCWC 2 cut(s) 232, 421
BslI CCNNNNNNNGG 2 cut(s) 511, 653
BsmAI GTCTC 2 cut(s) 247, 631
BsmBI CGTCTC 1 cut(s) 247
BsmI GAATGC 1 cut(s) 765
BsnI GGCC 4 cut(s) 504, 599, 660, 775
Bsp119I TTCGAA 1 cut(s) 757
Bsp1286I GDGCHC 2 cut(s) 232, 421
Bsp1407I TGTACA 2 cut(s) 329, 528
Bsp143I GATC 5 cut(s) 51, 84, 90, 97, 703
Bsp19I CCATGG 1 cut(s) 106
BspACI CCGC 1 cut(s) 561
BspANI GGCC 4 cut(s) 504, 599, 660, 775
BspCNI CTCAG 2 cut(s) 685, 802
BspDI ATCGAT 1 cut(s) 96
BspHI TCATGA 2 cut(s) 57, 87
BspLI GGNNCC 1 cut(s) 180
BspMAI CTGCAG 1 cut(s) 364
BspQI GCTCTTC 1 cut(s) 220
BspT104I TTCGAA 1 cut(s) 757
BspT107I GGYRCC 1 cut(s) 178
BsrGI TGTACA 2 cut(s) 329, 528
BsrI ACTGG 3 cut(s) 304, 457, 600
BssECI CCNNGG 4 cut(s) 106, 208, 499, 661
BssMI GATC 5 cut(s) 51, 84, 90, 97, 703
BssNI GRCGYC 1 cut(s) 179
BssT1I CCWWGG 2 cut(s) 106, 499
Bst2UI CCWGG 3 cut(s) 210, 247, 663
Bst4CI ACNGT 1 cut(s) 353
Bst6I CTCTTC 1 cut(s) 220
BstACI GRCGYC 1 cut(s) 179
BstAUI TGTACA 2 cut(s) 329, 528
BstBAI YACGTR 1 cut(s) 440
BstBI TTCGAA 1 cut(s) 757
BstC8I GCNNGC 2 cut(s) 177, 321
BstDEI CTNAG 2 cut(s) 672, 789
BstDSI CCRYGG 1 cut(s) 106
BstF5I GGATG 1 cut(s) 397
BstH2I RGCGCY 2 cut(s) 182, 871
BstHHI GCGC 3 cut(s) 143, 181, 870
BstKTI GATC 5 cut(s) 54, 87, 93, 100, 706
BstMAI GTCTC 2 cut(s) 247, 631
BstMBI GATC 5 cut(s) 51, 84, 90, 97, 703
BstMWI GCNNNNNNNGC 4 cut(s) 187, 219, 722, 731
BstNI CCWGG 3 cut(s) 210, 247, 663
BstNSI RCATGY 1 cut(s) 470
BstSCI CCNGG 3 cut(s) 208, 245, 661
BstSFI CTRYAG 1 cut(s) 360
BstV1I GCAGC 7 cut(s) 106, 346, 446, 457, 562, 722, 725
BstV2I GAAGAC 1 cut(s) 489
Bsu15I ATCGAT 1 cut(s) 96
BsuRI GGCC 4 cut(s) 504, 599, 660, 775
BsuTUI ATCGAT 1 cut(s) 96
BtgI CCRYGG 1 cut(s) 106
BtsCI GGATG 1 cut(s) 397
BtsI GCAGTG 2 cut(s) 369, 741
BtsIMutI CAGTG 3 cut(s) 369, 741, 871
Cac8I GCNNGC 2 cut(s) 177, 321
CciI TCATGA 2 cut(s) 57, 87
CfoI GCGC 3 cut(s) 143, 181, 870
Cfr13I GGNCC 4 cut(s) 287, 503, 659, 773
ClaI ATCGAT 1 cut(s) 96
Csp6I GTAC 6 cut(s) 4, 157, 297, 330, 349, 529
CviAII CATG 9 cut(s) 49, 58, 64, 82, 88, 107, 132, 413, 467
CviQI GTAC 6 cut(s) 4, 157, 297, 330, 349, 529
DdeI CTNAG 2 cut(s) 672, 789
DinI GGCGCC 1 cut(s) 180
DpnI GATC 5 cut(s) 53, 86, 92, 99, 705
DpnII GATC 5 cut(s) 51, 84, 90, 97, 703
EaeI YGGCCR 1 cut(s) 597
Eam1104I CTCTTC 1 cut(s) 220
EarI CTCTTC 1 cut(s) 220
EciI GGCGGA 1 cut(s) 576
Ecl136II GAGCTC 1 cut(s) 230
Eco130I CCWWGG 2 cut(s) 106, 499
Eco24I GRGCYC 1 cut(s) 232
Eco47I GGWCC 1 cut(s) 287
Eco53kI GAGCTC 1 cut(s) 230
Eco57I CTGAAG 2 cut(s) 202, 568
EcoICRI GAGCTC 1 cut(s) 230
EcoO109I RGGNCCY 1 cut(s) 773
EcoRI GAATTC 1 cut(s) 127
EcoRII CCWGG 3 cut(s) 208, 245, 661
EcoT14I CCWWGG 2 cut(s) 106, 499
EcoT38I GRGCYC 1 cut(s) 232
EgeI GGCGCC 1 cut(s) 180
EheI GGCGCC 1 cut(s) 180
ErhI CCWWGG 2 cut(s) 106, 499
Esp3I CGTCTC 1 cut(s) 247
FaeI CATG 9 cut(s) 52, 61, 67, 85, 91, 110, 135, 416, 470
FatI CATG 9 cut(s) 48, 57, 63, 81, 87, 106, 131, 412, 466
FbaI TGATCA 3 cut(s) 51, 84, 90
Fnu4HI GCNGC 7 cut(s) 120, 360, 460, 471, 576, 711, 714
FokI GGATG 1 cut(s) 404
FriOI GRGCYC 1 cut(s) 232
Fsp4HI GCNGC 7 cut(s) 120, 360, 460, 471, 576, 711, 714
GlaI GCGC 3 cut(s) 142, 180, 869
GluI GCNGC 7 cut(s) 120, 360, 460, 471, 576, 711, 714
GsuI CTGGAG 1 cut(s) 229
HaeII RGCGCY 2 cut(s) 182, 871
HaeIII GGCC 4 cut(s) 504, 599, 660, 775
HhaI GCGC 3 cut(s) 143, 181, 870
Hin1I GRCGYC 1 cut(s) 179
Hin1II CATG 9 cut(s) 52, 61, 67, 85, 91, 110, 135, 416, 470
Hin6I GCGC 3 cut(s) 141, 179, 868
HinP1I GCGC 3 cut(s) 141, 179, 868
HinfI GANTC 3 cut(s) 24, 554, 666
HphI GGTGA 7 cut(s) 67, 262, 407, 418, 647, 700, 805
Hpy166II GTNNAC 5 cut(s) 157, 287, 330, 332, 826
Hpy188III TCNNGA 6 cut(s) 58, 88, 425, 479, 509, 823
Hpy8I GTNNAC 5 cut(s) 157, 287, 330, 332, 826
HpyAV CCTTC 2 cut(s) 145, 692
HpyCH4III ACNGT 1 cut(s) 353
HpyCH4IV ACGT 1 cut(s) 439
HpyCH4V TGCA 6 cut(s) 122, 362, 473, 578, 710, 752
HpyF10VI GCNNNNNNNGC 4 cut(s) 187, 219, 722, 731
HpyF3I CTNAG 2 cut(s) 672, 789
HpySE526I ACGT 1 cut(s) 439
Hsp92I GRCGYC 1 cut(s) 179
Hsp92II CATG 9 cut(s) 52, 61, 67, 85, 91, 110, 135, 416, 470
HspAI GCGC 3 cut(s) 141, 179, 868
KasI GGCGCC 1 cut(s) 178
Ksp22I TGATCA 3 cut(s) 51, 84, 90
Kzo9I GATC 5 cut(s) 51, 84, 90, 97, 703
LguI GCTCTTC 1 cut(s) 220
LmnI GCTCC 5 cut(s) 235, 416, 514, 722, 742
Lsp1109I GCAGC 7 cut(s) 106, 346, 446, 457, 562, 722, 725
LweI GCATC 2 cut(s) 306, 739
MaeII ACGT 1 cut(s) 439
MaeIII GTNAC 2 cut(s) 341, 364
MalI GATC 5 cut(s) 53, 86, 92, 99, 705
MboI GATC 5 cut(s) 51, 84, 90, 97, 703
MboII GAAGA 5 cut(s) 237, 440, 494, 827, 874
MhlI GDGCHC 2 cut(s) 232, 421
MluCI AATT 5 cut(s) 127, 250, 474, 568, 642
Mly113I GGCGCC 1 cut(s) 179
MmeI TCCRAC 1 cut(s) 700
MnlI CCTC 6 cut(s) 246, 382, 505, 516, 764, 841
MseI TTAA 1 cut(s) 495
MslI CAYNNNNRTG 5 cut(s) 47, 62, 80, 86, 105
MspA1I CMGCKG 1 cut(s) 119
MspR9I CCNGG 3 cut(s) 210, 247, 663
Mva1269I GAATGC 1 cut(s) 765
MvaI CCWGG 3 cut(s) 210, 247, 663
MwoI GCNNNNNNNGC 4 cut(s) 187, 219, 722, 731
NarI GGCGCC 1 cut(s) 179
NcoI CCATGG 1 cut(s) 106
NdeII GATC 5 cut(s) 51, 84, 90, 97, 703
NlaIII CATG 9 cut(s) 52, 61, 67, 85, 91, 110, 135, 416, 470
NlaIV GGNNCC 1 cut(s) 180
NmuCI GTSAC 2 cut(s) 341, 364
NspI RCATGY 1 cut(s) 470
NspV TTCGAA 1 cut(s) 757
PagI TCATGA 2 cut(s) 57, 87
PciSI GCTCTTC 1 cut(s) 220
PctI GAATGC 1 cut(s) 765
PfeI GAWTC 3 cut(s) 24, 554, 666
PfoI TCCNGGA 1 cut(s) 245
PkrI GCNGC 7 cut(s) 121, 361, 461, 472, 577, 712, 715
PluTI GGCGCC 1 cut(s) 182
Ppu21I YACGTR 1 cut(s) 440
Psp124BI GAGCTC 1 cut(s) 232
Psp6I CCWGG 3 cut(s) 208, 245, 661
PspGI CCWGG 3 cut(s) 208, 245, 661
PspN4I GGNNCC 1 cut(s) 180
PspPI GGNCC 4 cut(s) 287, 503, 659, 773
PstI CTGCAG 1 cut(s) 364
PvuII CAGCTG 1 cut(s) 119
RsaI GTAC 6 cut(s) 5, 158, 298, 331, 350, 530
RsaNI GTAC 6 cut(s) 4, 157, 297, 330, 349, 529
RseI CAYNNNNRTG 5 cut(s) 47, 62, 80, 86, 105
SacI GAGCTC 1 cut(s) 232
SapI GCTCTTC 1 cut(s) 220
SaqAI TTAA 1 cut(s) 495
SatI GCNGC 7 cut(s) 120, 360, 460, 471, 576, 711, 714
Sau3AI GATC 5 cut(s) 51, 84, 90, 97, 703
Sau96I GGNCC 4 cut(s) 287, 503, 659, 773
ScrFI CCNGG 3 cut(s) 210, 247, 663
SduI GDGCHC 2 cut(s) 232, 421
SfaNI GCATC 2 cut(s) 306, 739
SfcI CTRYAG 1 cut(s) 360
SfoI GGCGCC 1 cut(s) 180
SfuI TTCGAA 1 cut(s) 757
SinI GGWCC 1 cut(s) 287
SmiMI CAYNNNNRTG 5 cut(s) 47, 62, 80, 86, 105
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
Sse9I AATT 5 cut(s) 127, 250, 474, 568, 642
SsiI CCGC 1 cut(s) 561
SspDI GGCGCC 1 cut(s) 178
SstI GAGCTC 1 cut(s) 232
StyD4I CCNGG 3 cut(s) 208, 245, 661
StyI CCWWGG 2 cut(s) 106, 499
TaaI ACNGT 1 cut(s) 353
TaiI ACGT 1 cut(s) 442
TaqI TCGA 2 cut(s) 96, 757
TaqII GACCGA 1 cut(s) 551
TasI AATT 5 cut(s) 127, 250, 474, 568, 642
TatI WGTACW 3 cut(s) 329, 348, 528
TfiI GAWTC 3 cut(s) 24, 554, 666
Tru1I TTAA 1 cut(s) 495
Tru9I TTAA 1 cut(s) 495
TscAI CASTG 2 cut(s) 369, 741
TseFI GTSAC 2 cut(s) 341, 364
TseI GCWGC 7 cut(s) 119, 359, 459, 470, 575, 710, 713
Tsp45I GTSAC 2 cut(s) 341, 364
TspDTI ATGAA 2 cut(s) 120, 273
TspRI CASTG 2 cut(s) 369, 741
VpaK11BI GGWCC 1 cut(s) 287
XapI RAATTY 3 cut(s) 127, 250, 642
XceI RCATGY 1 cut(s) 470
XcmI CCANNNNNNNNNTGG 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.