MD10G1263700.v1.1

urease accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0012327
Physical Location & Seq
Forward (+)
35770739 .. 35773668
2930 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1263700.v1.1.491

Sequence Viewer

Length: 843 bp
ATGGCTGACCACCACCATGATCATCATGACCATGACCATGACCATCATCACCACCATGATCATGATCATAGCCATGGGAGTTCAGCTGCAACGAATTCATGGGTGGGCTCAGATGGGAAGGTGTACCATAGCCACGATGGGTTGGCGCCACACTCACACGAACCCATATACTCCCCTGGCTACTTCAGCAGAAGAGCTCCACCTCTCCTCTCCAGGAATTTCAATGAAAGGGCTTTCACCATTGGCATTGGTGGACCTGTTGGTACTGGGAAAACTGCTTTGATGCTCGCTCTGTGTACACTTTTGCGTGACAAGTACAGTCTTGCTGCAGTGACAAATGATATATTCACAAAAGAGGATGGTGAGTTCTTGGTGAAGCATGGAGCACTTCCCGAAGAAAGAATACGTGCTGTGGAAACTGGGGGCTGCCCACATGCTGCAATTCGTGAAGACATCAGTATTAACCTTGGCCCTCTTGAGGAGCTTTCTAACTTGTACAAGACCGACATACTGCTTTGTGAATCAGGCGGAGATAATTTAGCCGCGAACTTCAGCAGGGAACTGGCCGACTATATTATTTACATTATAGATGTCTCTGCTGGTGATAAAATTCCACGAAAAGGTGGCCCTGGAATCACTCAGGCTGACCTTCTGGTGATAAACAAGACTGATCTTGCAGCAGCGGTTGGAGCTGATTTGGCAGTGATGGAGCGTGATGCACTTCGAATGCGAGATGGAGGGCCTTTTGTCTTTGCTCAGGTGAAGCACGGAAAAGGAGTAGAGGAAATCGTGAACCACATATTACAGGCTTGGGAAGTAGCAACGGGGAAGAAGCGCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006807 GO:0008150 GO:0008152 GO:0043085 GO:0044093 GO:0050790 GO:0065007 GO:0065009
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cobW

Protein Analysis

281

Amino Acids

30.58

Weight (kDa)

6.02

Isoelectric Point (pI)

27.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 80 - 250 1.1e-35 CobW/HypB/UreG, nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012327)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34470 AT2G34470
fragaria_vesca FvH4_3g05780
malus_domestica MD05G1285700.v1.1 MD10G1263700.v1.1
prunus_persica Prupe.4G079000_v2.0.a1
pyrus_communis pycom10g21990
rosa_chinensis RchiOBHm_Chr5g0013721 RchiOBHm_Chr5g0013731 RchiOBHm_Chr5g0013741 RchiOBHm_Chr5g0013751
rosa_laevigata RLG00000032043
rosa_multiflora Rmu_ssc0000256.1_g000001
rosa_roxburghii Rroxscaffold_1G00062890
rosa_rugosa Rorug05G0012600
rosa_samantha Rh5AG106900 Rh5BG103000 Rh5DG101700
rosa_wichuraiana Rw5G009320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 145
AccII CGCG 1 cut(s) 545
AciI CCGC 3 cut(s) 528, 543, 683
AcoI YGGCCR 1 cut(s) 564
AcsI RAATTY 3 cut(s) 94, 217, 609
AcuI CTGAAG 2 cut(s) 169, 535
AcyI GRCGYC 1 cut(s) 146
AfaI GTAC 5 cut(s) 125, 265, 298, 317, 497
AfiI CCNNNNNNNGG 2 cut(s) 478, 620
AgsI TTSAA 1 cut(s) 223
AjnI CCWGG 3 cut(s) 175, 212, 628
AloI GAACNNNNNNTCC 2 cut(s) 350, 382
AluBI AGCT 4 cut(s) 86, 197, 484, 692
AluI AGCT 4 cut(s) 86, 197, 484, 692
Alw21I GWGCWC 2 cut(s) 199, 388
Alw26I GTCTC 1 cut(s) 598
AoxI GGCC 4 cut(s) 469, 564, 625, 740
ApeKI GCWGC 6 cut(s) 86, 326, 426, 437, 677, 680
ApoI RAATTY 3 cut(s) 94, 217, 609
AspLEI GCGC 2 cut(s) 148, 837
AspS9I GGNCC 4 cut(s) 254, 470, 626, 740
AsuHPI GGTGA 7 cut(s) 41, 229, 374, 385, 614, 667, 772
AsuII TTCGAA 1 cut(s) 724
AvaII GGWCC 1 cut(s) 254
BanI GGYRCC 1 cut(s) 145
BanII GRGCYC 2 cut(s) 110, 199
BarI GAAGNNNNNNTAC 2 cut(s) 387, 419
BbsI GAAGAC 1 cut(s) 456
Bbv12I GWGCWC 2 cut(s) 199, 388
BbvI GCAGC 6 cut(s) 73, 313, 413, 424, 689, 692
BccI CCATC 6 cut(s) 51, 107, 131, 353, 700, 728
BciT130I CCWGG 3 cut(s) 177, 214, 630
BclI TGATCA 3 cut(s) 19, 58, 64
BcoDI GTCTC 1 cut(s) 598
BfmI CTRYAG 1 cut(s) 327
BfoI RGCGCY 2 cut(s) 149, 838
BisI GCNGC 7 cut(s) 87, 327, 427, 438, 543, 678, 681
BlsI GCNGC 7 cut(s) 88, 328, 428, 439, 544, 679, 682
Bme1390I CCNGG 3 cut(s) 177, 214, 630
Bme18I GGWCC 1 cut(s) 254
BmgT120I GGNCC 4 cut(s) 254, 470, 626, 740
BmiI GGNNCC 1 cut(s) 147
BmrFI CCNGG 3 cut(s) 177, 214, 630
BmrI ACTGGG 2 cut(s) 276, 429
BmsI GCATC 2 cut(s) 273, 706
BmuI ACTGGG 2 cut(s) 276, 429
BpiI GAAGAC 1 cut(s) 456
BpmI CTGGAG 1 cut(s) 196
Bpu10I CCTNAGC 1 cut(s) 756
Bpu14I TTCGAA 1 cut(s) 724
BpuEI CTTGAG 1 cut(s) 497
BsaAI YACGTR 1 cut(s) 407
BsaBI GATNNNNATC 1 cut(s) 63
BsaHI GRCGYC 1 cut(s) 146
BsaJI CCNNGG 4 cut(s) 73, 175, 466, 628
Bsc4I CCNNNNNNNGG 2 cut(s) 478, 620
Bse1I ACTGG 3 cut(s) 271, 424, 567
Bse8I GATNNNNATC 1 cut(s) 63
BseBI CCWGG 3 cut(s) 177, 214, 630
BseDI CCNNGG 4 cut(s) 73, 175, 466, 628
BseGI GGATG 1 cut(s) 364
BseJI GATNNNNATC 1 cut(s) 63
BseLI CCNNNNNNNGG 2 cut(s) 478, 620
BseMII CTCAG 3 cut(s) 123, 653, 770
BseNI ACTGG 3 cut(s) 271, 424, 567
BseRI GAGGAG 2 cut(s) 197, 494
BseXI GCAGC 6 cut(s) 73, 313, 413, 424, 689, 692
Bsh1236I CGCG 1 cut(s) 545
BshFI GGCC 4 cut(s) 471, 566, 627, 742
BshNI GGYRCC 1 cut(s) 145
BsiHKAI GWGCWC 2 cut(s) 199, 388
BslI CCNNNNNNNGG 2 cut(s) 478, 620
BsmAI GTCTC 1 cut(s) 598
BsmI GAATGC 1 cut(s) 732
BsnI GGCC 4 cut(s) 471, 566, 627, 742
Bsp119I TTCGAA 1 cut(s) 724
Bsp1286I GDGCHC 3 cut(s) 110, 199, 388
Bsp1407I TGTACA 2 cut(s) 296, 495
Bsp143I GATC 4 cut(s) 19, 58, 64, 670
Bsp19I CCATGG 1 cut(s) 73
BspACI CCGC 3 cut(s) 528, 543, 683
BspANI GGCC 4 cut(s) 471, 566, 627, 742
BspCNI CTCAG 3 cut(s) 122, 652, 769
BspFNI CGCG 1 cut(s) 545
BspHI TCATGA 2 cut(s) 25, 61
BspLI GGNNCC 1 cut(s) 147
BspMAI CTGCAG 1 cut(s) 331
BspQI GCTCTTC 1 cut(s) 187
BspT104I TTCGAA 1 cut(s) 724
BspT107I GGYRCC 1 cut(s) 145
BsrGI TGTACA 2 cut(s) 296, 495
BsrI ACTGG 3 cut(s) 271, 424, 567
BssECI CCNNGG 4 cut(s) 73, 175, 466, 628
BssMI GATC 4 cut(s) 19, 58, 64, 670
BssNI GRCGYC 1 cut(s) 146
BssT1I CCWWGG 2 cut(s) 73, 466
Bst2UI CCWGG 3 cut(s) 177, 214, 630
Bst4CI ACNGT 1 cut(s) 320
Bst6I CTCTTC 1 cut(s) 187
BstACI GRCGYC 1 cut(s) 146
BstAUI TGTACA 2 cut(s) 296, 495
BstBAI YACGTR 1 cut(s) 407
BstBI TTCGAA 1 cut(s) 724
BstC8I GCNNGC 1 cut(s) 288
BstDEI CTNAG 3 cut(s) 109, 639, 756
BstDSI CCRYGG 1 cut(s) 73
BstF5I GGATG 1 cut(s) 364
BstFNI CGCG 1 cut(s) 545
BstH2I RGCGCY 2 cut(s) 149, 838
BstHHI GCGC 2 cut(s) 148, 837
BstKTI GATC 4 cut(s) 22, 61, 67, 673
BstMAI GTCTC 1 cut(s) 598
BstMBI GATC 4 cut(s) 19, 58, 64, 670
BstMWI GCNNNNNNNGC 3 cut(s) 186, 689, 698
BstNI CCWGG 3 cut(s) 177, 214, 630
BstNSI RCATGY 1 cut(s) 437
BstSCI CCNGG 3 cut(s) 175, 212, 628
BstSFI CTRYAG 1 cut(s) 327
BstUI CGCG 1 cut(s) 545
BstV1I GCAGC 6 cut(s) 73, 313, 413, 424, 689, 692
BstV2I GAAGAC 1 cut(s) 456
BsuRI GGCC 4 cut(s) 471, 566, 627, 742
BtgI CCRYGG 1 cut(s) 73
BtsCI GGATG 1 cut(s) 364
BtsI GCAGTG 2 cut(s) 336, 708
BtsIMutI CAGTG 3 cut(s) 336, 708, 838
Cac8I GCNNGC 1 cut(s) 288
CciI TCATGA 2 cut(s) 25, 61
CfoI GCGC 2 cut(s) 148, 837
Cfr13I GGNCC 4 cut(s) 254, 470, 626, 740
Csp6I GTAC 5 cut(s) 124, 264, 297, 316, 496
CviQI GTAC 5 cut(s) 124, 264, 297, 316, 496
DdeI CTNAG 3 cut(s) 109, 639, 756
DinI GGCGCC 1 cut(s) 147
DpnI GATC 4 cut(s) 21, 60, 66, 672
DpnII GATC 4 cut(s) 19, 58, 64, 670
EaeI YGGCCR 1 cut(s) 564
Eam1104I CTCTTC 1 cut(s) 187
EarI CTCTTC 1 cut(s) 187
EciI GGCGGA 1 cut(s) 543
Ecl136II GAGCTC 1 cut(s) 197
Eco130I CCWWGG 2 cut(s) 73, 466
Eco24I GRGCYC 2 cut(s) 110, 199
Eco47I GGWCC 1 cut(s) 254
Eco53kI GAGCTC 1 cut(s) 197
Eco57I CTGAAG 2 cut(s) 169, 535
EcoICRI GAGCTC 1 cut(s) 197
EcoO109I RGGNCCY 1 cut(s) 740
EcoRI GAATTC 1 cut(s) 94
EcoRII CCWGG 3 cut(s) 175, 212, 628
EcoT14I CCWWGG 2 cut(s) 73, 466
EcoT38I GRGCYC 2 cut(s) 110, 199
EgeI GGCGCC 1 cut(s) 147
EheI GGCGCC 1 cut(s) 147
ErhI CCWWGG 2 cut(s) 73, 466
FbaI TGATCA 3 cut(s) 19, 58, 64
Fnu4HI GCNGC 7 cut(s) 87, 327, 427, 438, 543, 678, 681
FokI GGATG 1 cut(s) 371
FriOI GRGCYC 2 cut(s) 110, 199
Fsp4HI GCNGC 7 cut(s) 87, 327, 427, 438, 543, 678, 681
GlaI GCGC 2 cut(s) 147, 836
GluI GCNGC 7 cut(s) 87, 327, 427, 438, 543, 678, 681
GsuI CTGGAG 1 cut(s) 196
HaeII RGCGCY 2 cut(s) 149, 838
HaeIII GGCC 4 cut(s) 471, 566, 627, 742
HhaI GCGC 2 cut(s) 148, 837
Hin1I GRCGYC 1 cut(s) 146
Hin6I GCGC 2 cut(s) 146, 835
HinP1I GCGC 2 cut(s) 146, 835
HinfI GANTC 2 cut(s) 521, 633
HphI GGTGA 7 cut(s) 41, 229, 374, 385, 614, 667, 772
Hpy166II GTNNAC 5 cut(s) 124, 254, 297, 299, 793
Hpy188I TCNGA 1 cut(s) 112
Hpy188III TCNNGA 6 cut(s) 26, 62, 392, 446, 476, 790
Hpy8I GTNNAC 5 cut(s) 124, 254, 297, 299, 793
HpyAV CCTTC 2 cut(s) 112, 659
HpyCH4III ACNGT 1 cut(s) 320
HpyCH4IV ACGT 1 cut(s) 406
HpyCH4V TGCA 5 cut(s) 89, 329, 440, 677, 719
HpyF10VI GCNNNNNNNGC 3 cut(s) 186, 689, 698
HpyF3I CTNAG 3 cut(s) 109, 639, 756
HpySE526I ACGT 1 cut(s) 406
Hsp92I GRCGYC 1 cut(s) 146
HspAI GCGC 2 cut(s) 146, 835
KasI GGCGCC 1 cut(s) 145
Ksp22I TGATCA 3 cut(s) 19, 58, 64
Kzo9I GATC 4 cut(s) 19, 58, 64, 670
LguI GCTCTTC 1 cut(s) 187
LmnI GCTCC 5 cut(s) 202, 383, 481, 689, 709
Lsp1109I GCAGC 6 cut(s) 73, 313, 413, 424, 689, 692
LweI GCATC 2 cut(s) 273, 706
MaeII ACGT 1 cut(s) 406
MaeIII GTNAC 2 cut(s) 308, 331
MalI GATC 4 cut(s) 21, 60, 66, 672
MboI GATC 4 cut(s) 19, 58, 64, 670
MboII GAAGA 4 cut(s) 204, 407, 461, 841
MhlI GDGCHC 3 cut(s) 110, 199, 388
MluCI AATT 5 cut(s) 94, 217, 441, 535, 609
Mly113I GGCGCC 1 cut(s) 146
MmeI TCCRAC 1 cut(s) 667
MnlI CCTC 7 cut(s) 213, 218, 349, 472, 483, 731, 775
MseI TTAA 1 cut(s) 462
MslI CAYNNNNRTG 6 cut(s) 15, 30, 36, 54, 60, 72
MspA1I CMGCKG 2 cut(s) 86, 683
MspR9I CCNGG 3 cut(s) 177, 214, 630
Mva1269I GAATGC 1 cut(s) 732
MvaI CCWGG 3 cut(s) 177, 214, 630
MvnI CGCG 1 cut(s) 545
MwoI GCNNNNNNNGC 3 cut(s) 186, 689, 698
NarI GGCGCC 1 cut(s) 146
NcoI CCATGG 1 cut(s) 73
NdeII GATC 4 cut(s) 19, 58, 64, 670
NlaIV GGNNCC 1 cut(s) 147
NmuCI GTSAC 2 cut(s) 308, 331
NspI RCATGY 1 cut(s) 437
NspV TTCGAA 1 cut(s) 724
PagI TCATGA 2 cut(s) 25, 61
PciSI GCTCTTC 1 cut(s) 187
PctI GAATGC 1 cut(s) 732
PfeI GAWTC 2 cut(s) 521, 633
PfoI TCCNGGA 1 cut(s) 212
PkrI GCNGC 7 cut(s) 88, 328, 428, 439, 544, 679, 682
PluTI GGCGCC 1 cut(s) 149
Ppu21I YACGTR 1 cut(s) 407
Psp124BI GAGCTC 1 cut(s) 199
Psp6I CCWGG 3 cut(s) 175, 212, 628
PspGI CCWGG 3 cut(s) 175, 212, 628
PspN4I GGNNCC 1 cut(s) 147
PspPI GGNCC 4 cut(s) 254, 470, 626, 740
PstI CTGCAG 1 cut(s) 331
PvuII CAGCTG 1 cut(s) 86
RsaI GTAC 5 cut(s) 125, 265, 298, 317, 497
RsaNI GTAC 5 cut(s) 124, 264, 297, 316, 496
RseI CAYNNNNRTG 6 cut(s) 15, 30, 36, 54, 60, 72
SacI GAGCTC 1 cut(s) 199
SapI GCTCTTC 1 cut(s) 187
SaqAI TTAA 1 cut(s) 462
SatI GCNGC 7 cut(s) 87, 327, 427, 438, 543, 678, 681
Sau3AI GATC 4 cut(s) 19, 58, 64, 670
Sau96I GGNCC 4 cut(s) 254, 470, 626, 740
ScrFI CCNGG 3 cut(s) 177, 214, 630
SduI GDGCHC 3 cut(s) 110, 199, 388
SfaNI GCATC 2 cut(s) 273, 706
SfcI CTRYAG 1 cut(s) 327
SfoI GGCGCC 1 cut(s) 147
SfuI TTCGAA 1 cut(s) 724
SinI GGWCC 1 cut(s) 254
SmiMI CAYNNNNRTG 6 cut(s) 15, 30, 36, 54, 60, 72
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
Sse9I AATT 5 cut(s) 94, 217, 441, 535, 609
SsiI CCGC 3 cut(s) 528, 543, 683
SspDI GGCGCC 1 cut(s) 145
SstI GAGCTC 1 cut(s) 199
StyD4I CCNGG 3 cut(s) 175, 212, 628
StyI CCWWGG 2 cut(s) 73, 466
TaaI ACNGT 1 cut(s) 320
TaiI ACGT 1 cut(s) 409
TaqI TCGA 1 cut(s) 724
TaqII GACCGA 1 cut(s) 518
TasI AATT 5 cut(s) 94, 217, 441, 535, 609
TatI WGTACW 3 cut(s) 296, 315, 495
TauI GCSGC 1 cut(s) 545
TfiI GAWTC 2 cut(s) 521, 633
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TscAI CASTG 2 cut(s) 336, 708
TseFI GTSAC 2 cut(s) 308, 331
TseI GCWGC 6 cut(s) 86, 326, 426, 437, 677, 680
Tsp45I GTSAC 2 cut(s) 308, 331
TspDTI ATGAA 2 cut(s) 87, 240
TspGWI ACGGA 1 cut(s) 783
TspRI CASTG 2 cut(s) 336, 708
VpaK11BI GGWCC 1 cut(s) 254
XapI RAATTY 3 cut(s) 94, 217, 609
XceI RCATGY 1 cut(s) 437
XcmI CCANNNNNNNNNTGG 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.