RchiOBHm_Chr5g0013731

urease accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0012327
Physical Location & Seq
Reverse (-)
9283798 .. 9285136
1339 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29423

Sequence Viewer

Length: 444 bp
ATGTGGGATATAAAGATTGCACTGATGAAGAAGAACAAGTGTGATTATTGCAGAATACGTGCTGTGGAAACTGGGGGATGCCCTCATGCTGCAATTCGCGAAGACATCAGCATTAATCTAGGCCCTATTGAGGAGCTTTCTAACTTGTACAAGGCAGACATACTTCTTTGTGAATCAGGCGGAGATAATTTAGCTGCGAACTTCAGCAGGGAGCTGGCTGTCTATATTATTTACATCATAGATGTATCTGGTGGTGATAAAATTCCACGAAAAGGTGGCCCTGGAATCACTCAAGCTGACCTTCTGTTGGAGCGTGACGCACTTCGAATGCGAGATAGAGGGCCATTTGTCTTTGCTCAGGTGAAACACGGGCAAGGAGTTGAGGAAATCGTGAACCACATAATACAGGCTTGGGAACTAGCAACAGGAAATAAGCGCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006807 GO:0008150 GO:0008152 GO:0043085 GO:0044093 GO:0050790 GO:0065007 GO:0065009
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cobW

Protein Analysis

147

Amino Acids

16.34

Weight (kDa)

6.42

Isoelectric Point (pI)

29.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 8 - 103 3.3e-11 CobW/HypB/UreG, nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012327)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34470 AT2G34470
fragaria_vesca FvH4_3g05780
malus_domestica MD05G1285700.v1.1 MD10G1263700.v1.1
prunus_persica Prupe.4G079000_v2.0.a1
pyrus_communis pycom10g21990
rosa_chinensis RchiOBHm_Chr5g0013721 RchiOBHm_Chr5g0013731 RchiOBHm_Chr5g0013741 RchiOBHm_Chr5g0013751
rosa_laevigata RLG00000032043
rosa_multiflora Rmu_ssc0000256.1_g000001
rosa_roxburghii Rroxscaffold_1G00062890
rosa_rugosa Rorug05G0012600
rosa_samantha Rh5AG106900 Rh5BG103000 Rh5DG101700
rosa_wichuraiana Rw5G009320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 99
AciI CCGC 1 cut(s) 180
AcsI RAATTY 1 cut(s) 261
AcuI CTGAAG 1 cut(s) 187
AfaI GTAC 1 cut(s) 149
AfiI CCNNNNNNNGG 3 cut(s) 130, 272, 307
AjnI CCWGG 1 cut(s) 280
AluBI AGCT 4 cut(s) 136, 194, 214, 296
AluI AGCT 4 cut(s) 136, 194, 214, 296
AoxI GGCC 3 cut(s) 121, 277, 341
ApeKI GCWGC 2 cut(s) 89, 194
ApoI RAATTY 1 cut(s) 261
AseI ATTAAT 1 cut(s) 114
AspLEI GCGC 1 cut(s) 438
AspS9I GGNCC 3 cut(s) 122, 278, 341
AsuHPI GGTGA 2 cut(s) 266, 373
AsuII TTCGAA 1 cut(s) 325
BbsI GAAGAC 1 cut(s) 108
BbvI GCAGC 2 cut(s) 76, 181
BciT130I CCWGG 1 cut(s) 282
BfaI CTAG 2 cut(s) 119, 419
BfoI RGCGCY 1 cut(s) 439
BisI GCNGC 2 cut(s) 90, 195
BlsI GCNGC 2 cut(s) 91, 196
Bme1390I CCNGG 1 cut(s) 282
BmgT120I GGNCC 3 cut(s) 122, 278, 341
BmrFI CCNGG 1 cut(s) 282
BmrI ACTGGG 1 cut(s) 81
BmsI GCATC 1 cut(s) 68
BmuI ACTGGG 1 cut(s) 81
BpiI GAAGAC 1 cut(s) 108
Bpu10I CCTNAGC 1 cut(s) 357
Bpu14I TTCGAA 1 cut(s) 325
BpuEI CTTGAG 1 cut(s) 276
BsaAI YACGTR 1 cut(s) 59
BsaJI CCNNGG 1 cut(s) 280
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 272, 307
Bse1I ACTGG 1 cut(s) 76
BseBI CCWGG 1 cut(s) 282
BseDI CCNNGG 1 cut(s) 280
BseGI GGATG 1 cut(s) 83
BseLI CCNNNNNNNGG 3 cut(s) 130, 272, 307
BseMII CTCAG 1 cut(s) 371
BseNI ACTGG 1 cut(s) 76
BseRI GAGGAG 1 cut(s) 146
BseXI GCAGC 2 cut(s) 76, 181
Bsh1236I CGCG 1 cut(s) 99
BshFI GGCC 3 cut(s) 123, 279, 343
BslI CCNNNNNNNGG 3 cut(s) 130, 272, 307
BsmI GAATGC 1 cut(s) 333
BsnI GGCC 3 cut(s) 123, 279, 343
Bsp119I TTCGAA 1 cut(s) 325
Bsp1407I TGTACA 1 cut(s) 147
Bsp68I TCGCGA 1 cut(s) 99
BspACI CCGC 1 cut(s) 180
BspANI GGCC 3 cut(s) 123, 279, 343
BspCNI CTCAG 1 cut(s) 370
BspFNI CGCG 1 cut(s) 99
BspT104I TTCGAA 1 cut(s) 325
BsrGI TGTACA 1 cut(s) 147
BsrI ACTGG 1 cut(s) 76
BssECI CCNNGG 1 cut(s) 280
Bst2UI CCWGG 1 cut(s) 282
BstAUI TGTACA 1 cut(s) 147
BstBAI YACGTR 1 cut(s) 59
BstBI TTCGAA 1 cut(s) 325
BstC8I GCNNGC 1 cut(s) 216
BstDEI CTNAG 1 cut(s) 357
BstF5I GGATG 1 cut(s) 83
BstFNI CGCG 1 cut(s) 99
BstH2I RGCGCY 1 cut(s) 439
BstHHI GCGC 1 cut(s) 438
BstNI CCWGG 1 cut(s) 282
BstSCI CCNGG 1 cut(s) 280
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 2 cut(s) 76, 181
BstV2I GAAGAC 1 cut(s) 108
BsuRI GGCC 3 cut(s) 123, 279, 343
BtsCI GGATG 1 cut(s) 83
BtsIMutI CAGTG 2 cut(s) 20, 439
BtuMI TCGCGA 1 cut(s) 99
Cac8I GCNNGC 1 cut(s) 216
CfoI GCGC 1 cut(s) 438
Cfr13I GGNCC 3 cut(s) 122, 278, 341
CseI GACGC 1 cut(s) 326
Csp6I GTAC 1 cut(s) 148
CviAII CATG 1 cut(s) 86
CviJI RGCY 9 cut(s) 123, 136, 194, 214, 218, 279, 296, 343, 410
CviKI_1 RGCY 9 cut(s) 123, 136, 194, 214, 218, 279, 296, 343, 410
CviQI GTAC 1 cut(s) 148
DdeI CTNAG 1 cut(s) 357
EciI GGCGGA 1 cut(s) 195
Eco57I CTGAAG 1 cut(s) 187
EcoO109I RGGNCCY 1 cut(s) 122
EcoRII CCWGG 1 cut(s) 280
FaeI CATG 1 cut(s) 89
FaiI YATR 6 cut(s) 11, 87, 161, 225, 239, 401
FalI AAGNNNNNCTT 2 cut(s) 285, 317
FatI CATG 1 cut(s) 85
Fnu4HI GCNGC 2 cut(s) 90, 195
FokI GGATG 1 cut(s) 90
Fsp4HI GCNGC 2 cut(s) 90, 195
FspBI CTAG 2 cut(s) 119, 419
GlaI GCGC 1 cut(s) 437
GluI GCNGC 2 cut(s) 90, 195
HaeII RGCGCY 1 cut(s) 439
HaeIII GGCC 3 cut(s) 123, 279, 343
HgaI GACGC 1 cut(s) 326
HhaI GCGC 1 cut(s) 438
Hin1II CATG 1 cut(s) 89
Hin6I GCGC 1 cut(s) 436
HinP1I GCGC 1 cut(s) 436
HinfI GANTC 2 cut(s) 173, 285
HphI GGTGA 2 cut(s) 266, 373
Hpy166II GTNNAC 1 cut(s) 394
Hpy188III TCNNGA 2 cut(s) 98, 391
Hpy8I GTNNAC 1 cut(s) 394
HpyAV CCTTC 1 cut(s) 311
HpyCH4IV ACGT 1 cut(s) 58
HpyCH4V TGCA 3 cut(s) 20, 51, 92
HpyF3I CTNAG 1 cut(s) 357
HpySE526I ACGT 1 cut(s) 58
Hsp92II CATG 1 cut(s) 89
HspAI GCGC 1 cut(s) 436
LmnI GCTCC 3 cut(s) 133, 211, 310
Lsp1109I GCAGC 2 cut(s) 76, 181
LweI GCATC 1 cut(s) 68
MaeI CTAG 2 cut(s) 119, 419
MaeII ACGT 1 cut(s) 58
MaeIII GTNAC 1 cut(s) 314
MboII GAAGA 3 cut(s) 40, 43, 113
MluCI AATT 3 cut(s) 93, 187, 261
MmeI TCCRAC 1 cut(s) 288
MnlI CCTC 4 cut(s) 93, 124, 332, 376
MseI TTAA 1 cut(s) 114
MspR9I CCNGG 1 cut(s) 282
Mva1269I GAATGC 1 cut(s) 333
MvaI CCWGG 1 cut(s) 282
MvnI CGCG 1 cut(s) 99
NlaIII CATG 1 cut(s) 89
NmuCI GTSAC 1 cut(s) 314
NruI TCGCGA 1 cut(s) 99
NspV TTCGAA 1 cut(s) 325
PctI GAATGC 1 cut(s) 333
PfeI GAWTC 2 cut(s) 173, 285
PkrI GCNGC 2 cut(s) 91, 196
Ppu21I YACGTR 1 cut(s) 59
PshBI ATTAAT 1 cut(s) 114
Psp6I CCWGG 1 cut(s) 280
PspGI CCWGG 1 cut(s) 280
PspPI GGNCC 3 cut(s) 122, 278, 341
RruI TCGCGA 1 cut(s) 99
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
SaqAI TTAA 1 cut(s) 114
SatI GCNGC 2 cut(s) 90, 195
Sau96I GGNCC 3 cut(s) 122, 278, 341
ScrFI CCNGG 1 cut(s) 282
SetI ASST 8 cut(s) 61, 138, 196, 216, 277, 298, 303, 363
SfaNI GCATC 1 cut(s) 68
SfuI TTCGAA 1 cut(s) 325
SmlI CTYRAG 1 cut(s) 291
SmoI CTYRAG 1 cut(s) 291
Sse9I AATT 3 cut(s) 93, 187, 261
SsiI CCGC 1 cut(s) 180
SspMI CTAG 2 cut(s) 119, 419
StyD4I CCNGG 1 cut(s) 280
TaiI ACGT 1 cut(s) 61
TaqI TCGA 1 cut(s) 325
TasI AATT 3 cut(s) 93, 187, 261
TatI WGTACW 1 cut(s) 147
TfiI GAWTC 2 cut(s) 173, 285
Tru1I TTAA 1 cut(s) 114
Tru9I TTAA 1 cut(s) 114
TscAI CASTG 1 cut(s) 27
TseFI GTSAC 1 cut(s) 314
TseI GCWGC 2 cut(s) 89, 194
Tsp45I GTSAC 1 cut(s) 314
TspDTI ATGAA 1 cut(s) 41
TspRI CASTG 1 cut(s) 27
VspI ATTAAT 1 cut(s) 114
XapI RAATTY 1 cut(s) 261
XspI CTAG 2 cut(s) 119, 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.