Rh5DG101700

urease accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0012327
Physical Location & Seq
Reverse (-)
9273952 .. 9280615
6664 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG101700.1

Sequence Viewer

Length: 600 bp
ATGGAATCCTGCTTCATGTTATACAGGAAAACTGCTTTGATGTTGGCTCTGTGTAAACTTTTGCGCGACAAGTACAGTCTTGCTGCAGTGACAAATGATATATTCACCAAAGAGGATGGTGAGTTCTTGGTGAAGCATGGAGCACTTCCTGAAGAGAGAATACGTGCTGTGGAAACTGGGGGATGCCCTCATGCTGCAATTCGCGAAGACATCAGCATTAATCTAGGCCCTCTTGAGGAGCTTTCTAACTTGTACAAGGCAGACATACTTCTTTGTGAATCAGGCGGAGATAATTTAGCTGCGAACTTCAGCAGGGAGCTGGCTGACTATATTATTTACATCATAGATGTATCTGGTGGTGATAAAATTCCACGAAAAGGTGGCCCTGGAATCACTCAAGCTGACCTTCTGGTGATAAACAAGACTGACCTTGCACAAGCAGTTGGAGCTGACTTGGCAGTGATGGAGCGTGACGCACTTCGAATGCGAGATGGAGGGCCATTTGTCTTTGCTCAGGTGAAGCACGGGCAAGGAGTTGAGGAAATCGTGAACCACATAATACAGGCTTGGGAACTAGCAACAGGAAATAAGCGCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006807 GO:0008150 GO:0008152 GO:0043085 GO:0044093 GO:0050790 GO:0065007 GO:0065009
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cobW

Protein Analysis

199

Amino Acids

21.81

Weight (kDa)

5.26

Isoelectric Point (pI)

25.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 10 - 169 4.1e-30 CobW/HypB/UreG, nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012327)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34470 AT2G34470
fragaria_vesca FvH4_3g05780
malus_domestica MD05G1285700.v1.1 MD10G1263700.v1.1
prunus_persica Prupe.4G079000_v2.0.a1
pyrus_communis pycom10g21990
rosa_chinensis RchiOBHm_Chr5g0013721 RchiOBHm_Chr5g0013731 RchiOBHm_Chr5g0013741 RchiOBHm_Chr5g0013751
rosa_laevigata RLG00000032043
rosa_multiflora Rmu_ssc0000256.1_g000001
rosa_roxburghii Rroxscaffold_1G00062890
rosa_rugosa Rorug05G0012600
rosa_samantha Rh5AG106900 Rh5BG103000 Rh5DG101700
rosa_wichuraiana Rw5G009320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 66, 204
AciI CCGC 1 cut(s) 285
AcsI RAATTY 1 cut(s) 366
AcuI CTGAAG 2 cut(s) 171, 292
AfaI GTAC 2 cut(s) 74, 254
AfiI CCNNNNNNNGG 2 cut(s) 235, 377
AjnI CCWGG 1 cut(s) 385
AloI GAACNNNNNNTCC 2 cut(s) 107, 139
AluBI AGCT 5 cut(s) 241, 299, 319, 401, 449
AluI AGCT 5 cut(s) 241, 299, 319, 401, 449
Alw21I GWGCWC 1 cut(s) 145
AoxI GGCC 3 cut(s) 226, 382, 497
ApeKI GCWGC 3 cut(s) 83, 194, 299
ApoI RAATTY 1 cut(s) 366
AseI ATTAAT 1 cut(s) 219
AspLEI GCGC 2 cut(s) 66, 594
AspS9I GGNCC 3 cut(s) 227, 383, 497
AsuHPI GGTGA 6 cut(s) 97, 131, 142, 371, 424, 529
AsuII TTCGAA 1 cut(s) 481
BarI GAAGNNNNNNTAC 2 cut(s) 144, 176
BbsI GAAGAC 1 cut(s) 213
Bbv12I GWGCWC 1 cut(s) 145
BbvI GCAGC 3 cut(s) 70, 181, 286
BccI CCATC 3 cut(s) 110, 457, 485
BciT130I CCWGG 1 cut(s) 387
BfaI CTAG 2 cut(s) 224, 575
BfmI CTRYAG 1 cut(s) 84
BfoI RGCGCY 1 cut(s) 595
BisI GCNGC 3 cut(s) 84, 195, 300
BlsI GCNGC 3 cut(s) 85, 196, 301
Bme1390I CCNGG 1 cut(s) 387
BmgT120I GGNCC 3 cut(s) 227, 383, 497
BmrFI CCNGG 1 cut(s) 387
BmrI ACTGGG 1 cut(s) 186
BmsI GCATC 1 cut(s) 173
BmuI ACTGGG 1 cut(s) 186
BpiI GAAGAC 1 cut(s) 213
Bpu10I CCTNAGC 1 cut(s) 513
Bpu14I TTCGAA 1 cut(s) 481
BpuEI CTTGAG 2 cut(s) 254, 381
BsaAI YACGTR 1 cut(s) 164
BsaJI CCNNGG 1 cut(s) 385
Bsc4I CCNNNNNNNGG 2 cut(s) 235, 377
Bse1I ACTGG 1 cut(s) 181
BseBI CCWGG 1 cut(s) 387
BseDI CCNNGG 1 cut(s) 385
BseGI GGATG 2 cut(s) 121, 188
BseLI CCNNNNNNNGG 2 cut(s) 235, 377
BseMII CTCAG 1 cut(s) 527
BseNI ACTGG 1 cut(s) 181
BseRI GAGGAG 1 cut(s) 251
BseXI GCAGC 3 cut(s) 70, 181, 286
Bsh1236I CGCG 2 cut(s) 66, 204
BshFI GGCC 3 cut(s) 228, 384, 499
BsiHKAI GWGCWC 1 cut(s) 145
BslI CCNNNNNNNGG 2 cut(s) 235, 377
BsmI GAATGC 1 cut(s) 489
BsnI GGCC 3 cut(s) 228, 384, 499
Bsp119I TTCGAA 1 cut(s) 481
Bsp1286I GDGCHC 1 cut(s) 145
Bsp1407I TGTACA 1 cut(s) 252
Bsp68I TCGCGA 1 cut(s) 204
BspACI CCGC 1 cut(s) 285
BspANI GGCC 3 cut(s) 228, 384, 499
BspCNI CTCAG 1 cut(s) 526
BspFNI CGCG 2 cut(s) 66, 204
BspMAI CTGCAG 1 cut(s) 88
BspT104I TTCGAA 1 cut(s) 481
BsrGI TGTACA 1 cut(s) 252
BsrI ACTGG 1 cut(s) 181
BssECI CCNNGG 1 cut(s) 385
Bst2UI CCWGG 1 cut(s) 387
Bst4CI ACNGT 1 cut(s) 77
Bst6I CTCTTC 1 cut(s) 147
BstAUI TGTACA 1 cut(s) 252
BstBAI YACGTR 1 cut(s) 164
BstBI TTCGAA 1 cut(s) 481
BstC8I GCNNGC 1 cut(s) 321
BstDEI CTNAG 1 cut(s) 513
BstF5I GGATG 2 cut(s) 121, 188
BstFNI CGCG 2 cut(s) 66, 204
BstH2I RGCGCY 1 cut(s) 595
BstHHI GCGC 2 cut(s) 66, 594
BstMWI GCNNNNNNNGC 2 cut(s) 446, 455
BstNI CCWGG 1 cut(s) 387
BstSCI CCNGG 1 cut(s) 385
BstSFI CTRYAG 1 cut(s) 84
BstUI CGCG 2 cut(s) 66, 204
BstV1I GCAGC 3 cut(s) 70, 181, 286
BstV2I GAAGAC 1 cut(s) 213
BsuRI GGCC 3 cut(s) 228, 384, 499
BtsCI GGATG 2 cut(s) 121, 188
BtsI GCAGTG 2 cut(s) 93, 465
BtsIMutI CAGTG 3 cut(s) 93, 465, 595
BtuMI TCGCGA 1 cut(s) 204
Cac8I GCNNGC 1 cut(s) 321
CfoI GCGC 2 cut(s) 66, 594
Cfr13I GGNCC 3 cut(s) 227, 383, 497
CseI GACGC 1 cut(s) 482
Csp6I GTAC 2 cut(s) 73, 253
CviAII CATG 3 cut(s) 16, 137, 191
CviQI GTAC 2 cut(s) 73, 253
DdeI CTNAG 1 cut(s) 513
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
EciI GGCGGA 1 cut(s) 300
Eco57I CTGAAG 2 cut(s) 171, 292
EcoO109I RGGNCCY 1 cut(s) 227
EcoRII CCWGG 1 cut(s) 385
FaeI CATG 3 cut(s) 19, 140, 194
FaiI YATR 9 cut(s) 17, 22, 101, 138, 192, 266, 330, 344, 557
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FatI CATG 3 cut(s) 15, 136, 190
Fnu4HI GCNGC 3 cut(s) 84, 195, 300
FokI GGATG 2 cut(s) 128, 195
Fsp4HI GCNGC 3 cut(s) 84, 195, 300
FspBI CTAG 2 cut(s) 224, 575
GlaI GCGC 2 cut(s) 65, 593
GluI GCNGC 3 cut(s) 84, 195, 300
HaeII RGCGCY 1 cut(s) 595
HaeIII GGCC 3 cut(s) 228, 384, 499
HgaI GACGC 1 cut(s) 482
HhaI GCGC 2 cut(s) 66, 594
Hin1II CATG 3 cut(s) 19, 140, 194
Hin6I GCGC 2 cut(s) 64, 592
HinP1I GCGC 2 cut(s) 64, 592
HinfI GANTC 3 cut(s) 5, 278, 390
HphI GGTGA 6 cut(s) 97, 131, 142, 371, 424, 529
Hpy166II GTNNAC 2 cut(s) 56, 550
Hpy188III TCNNGA 4 cut(s) 149, 203, 233, 547
Hpy8I GTNNAC 2 cut(s) 56, 550
HpyAV CCTTC 1 cut(s) 416
HpyCH4III ACNGT 1 cut(s) 77
HpyCH4IV ACGT 1 cut(s) 163
HpyCH4V TGCA 3 cut(s) 86, 197, 434
HpyF10VI GCNNNNNNNGC 2 cut(s) 446, 455
HpyF3I CTNAG 1 cut(s) 513
HpySE526I ACGT 1 cut(s) 163
Hsp92II CATG 3 cut(s) 19, 140, 194
HspAI GCGC 2 cut(s) 64, 592
LmnI GCTCC 5 cut(s) 140, 238, 316, 446, 466
Lsp1109I GCAGC 3 cut(s) 70, 181, 286
LweI GCATC 1 cut(s) 173
MaeI CTAG 2 cut(s) 224, 575
MaeII ACGT 1 cut(s) 163
MaeIII GTNAC 2 cut(s) 88, 470
MboII GAAGA 2 cut(s) 164, 218
MhlI GDGCHC 1 cut(s) 145
MluCI AATT 3 cut(s) 198, 292, 366
MmeI TCCRAC 1 cut(s) 424
MnlI CCTC 6 cut(s) 106, 198, 229, 240, 488, 532
MseI TTAA 1 cut(s) 219
MspR9I CCNGG 1 cut(s) 387
Mva1269I GAATGC 1 cut(s) 489
MvaI CCWGG 1 cut(s) 387
MvnI CGCG 2 cut(s) 66, 204
MwoI GCNNNNNNNGC 2 cut(s) 446, 455
NlaIII CATG 3 cut(s) 19, 140, 194
NmuCI GTSAC 2 cut(s) 88, 470
NruI TCGCGA 1 cut(s) 204
NspV TTCGAA 1 cut(s) 481
PctI GAATGC 1 cut(s) 489
PfeI GAWTC 3 cut(s) 5, 278, 390
PkrI GCNGC 3 cut(s) 85, 196, 301
Ppu21I YACGTR 1 cut(s) 164
PshBI ATTAAT 1 cut(s) 219
Psp6I CCWGG 1 cut(s) 385
PspGI CCWGG 1 cut(s) 385
PspPI GGNCC 3 cut(s) 227, 383, 497
PstI CTGCAG 1 cut(s) 88
RruI TCGCGA 1 cut(s) 204
RsaI GTAC 2 cut(s) 74, 254
RsaNI GTAC 2 cut(s) 73, 253
SaqAI TTAA 1 cut(s) 219
SatI GCNGC 3 cut(s) 84, 195, 300
Sau96I GGNCC 3 cut(s) 227, 383, 497
ScrFI CCNGG 1 cut(s) 387
SduI GDGCHC 1 cut(s) 145
SfaNI GCATC 1 cut(s) 173
SfcI CTRYAG 1 cut(s) 84
SfuI TTCGAA 1 cut(s) 481
SmlI CTYRAG 2 cut(s) 233, 396
SmoI CTYRAG 2 cut(s) 233, 396
Sse9I AATT 3 cut(s) 198, 292, 366
SsiI CCGC 1 cut(s) 285
SspMI CTAG 2 cut(s) 224, 575
StyD4I CCNGG 1 cut(s) 385
TaaI ACNGT 1 cut(s) 77
TaiI ACGT 1 cut(s) 166
TaqI TCGA 1 cut(s) 481
TasI AATT 3 cut(s) 198, 292, 366
TatI WGTACW 2 cut(s) 72, 252
TfiI GAWTC 3 cut(s) 5, 278, 390
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TscAI CASTG 2 cut(s) 93, 465
TseFI GTSAC 2 cut(s) 88, 470
TseI GCWGC 3 cut(s) 83, 194, 299
Tsp45I GTSAC 2 cut(s) 88, 470
TspDTI ATGAA 1 cut(s) 4
TspRI CASTG 2 cut(s) 93, 465
VspI ATTAAT 1 cut(s) 219
XapI RAATTY 1 cut(s) 366
XspI CTAG 2 cut(s) 224, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.