RchiOBHm_Chr5g0013721

urease accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0012327
Physical Location & Seq
Reverse (-)
9258319 .. 9259657
1339 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29422

Sequence Viewer

Length: 447 bp
ATGTGGGATATAAAGATTGCACTGATGAAGAAGAACAAGTCACTTCCTGAAGAGAGAATACGTTCTGTGGAAACTGGGGGATGCCCTCATGCTGCAATTCGCGAAGACATCAGCATTAATCTAGGCCCTCTTGAGGAGCTTTCTAACTTGTACAAGGCAGACATACTTCTTTGTGAATCAGGCGGAGATAATTTAGCTGCGAACTTCAGCAGGGAGCTGGCTGACTATATTATTTACATCATAGATGTATCTGGTGGTGATAAAATTCCACGAAAAGGTGGCCCTGGAATCACTCAAGCTGACCTTCTGTTGGAGCGTGACGCACTTCGAATGCGAGATGGAGGGCCATTTGTCTTTGCTCAGGTGAAACACGGGCAAGGAGTTGAGGAAATCGTGAACCACATAATACAGGCTTGGGAACTAGCAACAGGAAATAAGCGCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006807 GO:0008150 GO:0008152 GO:0043085 GO:0044093 GO:0050790 GO:0065007 GO:0065009
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cobW

Protein Analysis

148

Amino Acids

16.35

Weight (kDa)

5.57

Isoelectric Point (pI)

34.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 8 - 104 1.4e-12 CobW/HypB/UreG, nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012327)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34470 AT2G34470
fragaria_vesca FvH4_3g05780
malus_domestica MD05G1285700.v1.1 MD10G1263700.v1.1
prunus_persica Prupe.4G079000_v2.0.a1
pyrus_communis pycom10g21990
rosa_chinensis RchiOBHm_Chr5g0013721 RchiOBHm_Chr5g0013731 RchiOBHm_Chr5g0013741 RchiOBHm_Chr5g0013751
rosa_laevigata RLG00000032043
rosa_multiflora Rmu_ssc0000256.1_g000001
rosa_roxburghii Rroxscaffold_1G00062890
rosa_rugosa Rorug05G0012600
rosa_samantha Rh5AG106900 Rh5BG103000 Rh5DG101700
rosa_wichuraiana Rw5G009320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 102
AciI CCGC 1 cut(s) 183
AcsI RAATTY 1 cut(s) 264
AcuI CTGAAG 2 cut(s) 69, 190
AfaI GTAC 1 cut(s) 152
AfiI CCNNNNNNNGG 3 cut(s) 133, 275, 310
AjnI CCWGG 1 cut(s) 283
AluBI AGCT 4 cut(s) 139, 197, 217, 299
AluI AGCT 4 cut(s) 139, 197, 217, 299
AoxI GGCC 3 cut(s) 124, 280, 344
ApeKI GCWGC 2 cut(s) 92, 197
ApoI RAATTY 1 cut(s) 264
AseI ATTAAT 1 cut(s) 117
Asp700I GAANNNNTTC 1 cut(s) 61
AspLEI GCGC 1 cut(s) 441
AspS9I GGNCC 3 cut(s) 125, 281, 344
AsuHPI GGTGA 2 cut(s) 269, 376
AsuII TTCGAA 1 cut(s) 328
BarI GAAGNNNNNNTAC 2 cut(s) 42, 74
BbsI GAAGAC 1 cut(s) 111
BbvI GCAGC 2 cut(s) 79, 184
BccI CCATC 1 cut(s) 332
BciT130I CCWGG 1 cut(s) 285
BfaI CTAG 2 cut(s) 122, 422
BfoI RGCGCY 1 cut(s) 442
BisI GCNGC 2 cut(s) 93, 198
BlsI GCNGC 2 cut(s) 94, 199
Bme1390I CCNGG 1 cut(s) 285
BmgT120I GGNCC 3 cut(s) 125, 281, 344
BmrFI CCNGG 1 cut(s) 285
BmrI ACTGGG 1 cut(s) 84
BmsI GCATC 1 cut(s) 71
BmuI ACTGGG 1 cut(s) 84
BpiI GAAGAC 1 cut(s) 111
Bpu10I CCTNAGC 1 cut(s) 360
Bpu14I TTCGAA 1 cut(s) 328
BpuEI CTTGAG 2 cut(s) 152, 279
BsaJI CCNNGG 1 cut(s) 283
Bsc4I CCNNNNNNNGG 3 cut(s) 133, 275, 310
Bse1I ACTGG 1 cut(s) 79
BseBI CCWGG 1 cut(s) 285
BseDI CCNNGG 1 cut(s) 283
BseGI GGATG 1 cut(s) 86
BseLI CCNNNNNNNGG 3 cut(s) 133, 275, 310
BseMII CTCAG 1 cut(s) 374
BseNI ACTGG 1 cut(s) 79
BseRI GAGGAG 1 cut(s) 149
BseXI GCAGC 2 cut(s) 79, 184
Bsh1236I CGCG 1 cut(s) 102
BshFI GGCC 3 cut(s) 126, 282, 346
BslI CCNNNNNNNGG 3 cut(s) 133, 275, 310
BsmI GAATGC 1 cut(s) 336
BsnI GGCC 3 cut(s) 126, 282, 346
Bsp119I TTCGAA 1 cut(s) 328
Bsp1407I TGTACA 1 cut(s) 150
Bsp68I TCGCGA 1 cut(s) 102
BspACI CCGC 1 cut(s) 183
BspANI GGCC 3 cut(s) 126, 282, 346
BspCNI CTCAG 1 cut(s) 373
BspFNI CGCG 1 cut(s) 102
BspT104I TTCGAA 1 cut(s) 328
BsrGI TGTACA 1 cut(s) 150
BsrI ACTGG 1 cut(s) 79
BssECI CCNNGG 1 cut(s) 283
Bst2UI CCWGG 1 cut(s) 285
Bst6I CTCTTC 1 cut(s) 45
BstAUI TGTACA 1 cut(s) 150
BstBI TTCGAA 1 cut(s) 328
BstC8I GCNNGC 1 cut(s) 219
BstDEI CTNAG 1 cut(s) 360
BstF5I GGATG 1 cut(s) 86
BstFNI CGCG 1 cut(s) 102
BstH2I RGCGCY 1 cut(s) 442
BstHHI GCGC 1 cut(s) 441
BstNI CCWGG 1 cut(s) 285
BstSCI CCNGG 1 cut(s) 283
BstUI CGCG 1 cut(s) 102
BstV1I GCAGC 2 cut(s) 79, 184
BstV2I GAAGAC 1 cut(s) 111
BsuRI GGCC 3 cut(s) 126, 282, 346
BtsCI GGATG 1 cut(s) 86
BtsIMutI CAGTG 2 cut(s) 20, 442
BtuMI TCGCGA 1 cut(s) 102
Cac8I GCNNGC 1 cut(s) 219
CfoI GCGC 1 cut(s) 441
Cfr13I GGNCC 3 cut(s) 125, 281, 344
CseI GACGC 1 cut(s) 329
Csp6I GTAC 1 cut(s) 151
CviAII CATG 1 cut(s) 89
CviJI RGCY 9 cut(s) 126, 139, 197, 217, 221, 282, 299, 346, 413
CviKI_1 RGCY 9 cut(s) 126, 139, 197, 217, 221, 282, 299, 346, 413
CviQI GTAC 1 cut(s) 151
DdeI CTNAG 1 cut(s) 360
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
EciI GGCGGA 1 cut(s) 198
Eco57I CTGAAG 2 cut(s) 69, 190
EcoO109I RGGNCCY 1 cut(s) 125
EcoRII CCWGG 1 cut(s) 283
FaeI CATG 1 cut(s) 92
FaiI YATR 6 cut(s) 11, 90, 164, 228, 242, 404
FalI AAGNNNNNCTT 2 cut(s) 288, 320
FatI CATG 1 cut(s) 88
Fnu4HI GCNGC 2 cut(s) 93, 198
FokI GGATG 1 cut(s) 93
Fsp4HI GCNGC 2 cut(s) 93, 198
FspBI CTAG 2 cut(s) 122, 422
GlaI GCGC 1 cut(s) 440
GluI GCNGC 2 cut(s) 93, 198
HaeII RGCGCY 1 cut(s) 442
HaeIII GGCC 3 cut(s) 126, 282, 346
HgaI GACGC 1 cut(s) 329
HhaI GCGC 1 cut(s) 441
Hin1II CATG 1 cut(s) 92
Hin6I GCGC 1 cut(s) 439
HinP1I GCGC 1 cut(s) 439
HinfI GANTC 2 cut(s) 176, 288
HphI GGTGA 2 cut(s) 269, 376
Hpy166II GTNNAC 1 cut(s) 397
Hpy188III TCNNGA 4 cut(s) 47, 101, 131, 394
Hpy8I GTNNAC 1 cut(s) 397
HpyAV CCTTC 1 cut(s) 314
HpyCH4IV ACGT 1 cut(s) 61
HpyCH4V TGCA 2 cut(s) 20, 95
HpyF3I CTNAG 1 cut(s) 360
HpySE526I ACGT 1 cut(s) 61
Hsp92II CATG 1 cut(s) 92
HspAI GCGC 1 cut(s) 439
LmnI GCTCC 3 cut(s) 136, 214, 313
Lsp1109I GCAGC 2 cut(s) 79, 184
LweI GCATC 1 cut(s) 71
MaeI CTAG 2 cut(s) 122, 422
MaeII ACGT 1 cut(s) 61
MaeIII GTNAC 2 cut(s) 39, 317
MboII GAAGA 4 cut(s) 40, 43, 62, 116
MluCI AATT 3 cut(s) 96, 190, 264
MmeI TCCRAC 1 cut(s) 291
MnlI CCTC 5 cut(s) 96, 127, 138, 335, 379
MroXI GAANNNNTTC 1 cut(s) 61
MseI TTAA 1 cut(s) 117
MspR9I CCNGG 1 cut(s) 285
Mva1269I GAATGC 1 cut(s) 336
MvaI CCWGG 1 cut(s) 285
MvnI CGCG 1 cut(s) 102
NlaIII CATG 1 cut(s) 92
NmuCI GTSAC 2 cut(s) 39, 317
NruI TCGCGA 1 cut(s) 102
NspV TTCGAA 1 cut(s) 328
PctI GAATGC 1 cut(s) 336
PdmI GAANNNNTTC 1 cut(s) 61
PfeI GAWTC 2 cut(s) 176, 288
PkrI GCNGC 2 cut(s) 94, 199
PshBI ATTAAT 1 cut(s) 117
Psp6I CCWGG 1 cut(s) 283
PspGI CCWGG 1 cut(s) 283
PspPI GGNCC 3 cut(s) 125, 281, 344
RruI TCGCGA 1 cut(s) 102
RsaI GTAC 1 cut(s) 152
RsaNI GTAC 1 cut(s) 151
SaqAI TTAA 1 cut(s) 117
SatI GCNGC 2 cut(s) 93, 198
Sau96I GGNCC 3 cut(s) 125, 281, 344
ScrFI CCNGG 1 cut(s) 285
SetI ASST 8 cut(s) 64, 141, 199, 219, 280, 301, 306, 366
SfaNI GCATC 1 cut(s) 71
SfuI TTCGAA 1 cut(s) 328
SmlI CTYRAG 2 cut(s) 131, 294
SmoI CTYRAG 2 cut(s) 131, 294
Sse9I AATT 3 cut(s) 96, 190, 264
SsiI CCGC 1 cut(s) 183
SspMI CTAG 2 cut(s) 122, 422
StyD4I CCNGG 1 cut(s) 283
TaiI ACGT 1 cut(s) 64
TaqI TCGA 1 cut(s) 328
TasI AATT 3 cut(s) 96, 190, 264
TatI WGTACW 1 cut(s) 150
TfiI GAWTC 2 cut(s) 176, 288
Tru1I TTAA 1 cut(s) 117
Tru9I TTAA 1 cut(s) 117
TscAI CASTG 1 cut(s) 27
TseFI GTSAC 2 cut(s) 39, 317
TseI GCWGC 2 cut(s) 92, 197
Tsp45I GTSAC 2 cut(s) 39, 317
TspDTI ATGAA 1 cut(s) 41
TspRI CASTG 1 cut(s) 27
VspI ATTAAT 1 cut(s) 117
XapI RAATTY 1 cut(s) 264
XmnI GAANNNNTTC 1 cut(s) 61
XspI CTAG 2 cut(s) 122, 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.