RchiOBHm_Chr5g0013741

urease accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0012327
Physical Location & Seq
Reverse (-)
9322220 .. 9323643
1424 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29424

Sequence Viewer

Length: 522 bp
ATGTTTCTAGTGACAAATGATATATTCACCAAAGAGGATGGTGAGTTCTTGGTGAAGCATGGAGCACTTCCTGAAGAGAGAATACGTGCTGTGGAAACTGGGGGATGCCCTCATGCTGCAATTCGCGAAGACATCAGCATTAATCTAGGCCCTCTTGAGGAGCTTTCTAACTTGTACAAGGCAGACATACTTCTTTGTGAATCAGGCGGAGATAATTTAGCTGCGAACTTCAGCAGGGAGCTGGCTGACTATATTATTTACATCATAGATGTATCTGGTGGTGATAAAATTCCACGAAAAGGTGGCCCTGGAATCACTCAAGCTGACCTTCTGGTGATAAACAAGACTGACCTTGCACAAGCAGTTGGAGCTGACTTGGCAGTGATGGAGCGTGACGCACTTCGAATGCGAGATGGAGGGCCATTTGTCTTTGCTCAGGTGAAGCACGGGCAAGGAGTTGAGGAAATCTTGAACCACATAATACAGGCTTGGGAACTAGCAACAGGAAATAAGCGCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006807 GO:0008150 GO:0008152 GO:0043085 GO:0044093 GO:0050790 GO:0065007 GO:0065009
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cobW

Protein Analysis

173

Amino Acids

18.85

Weight (kDa)

4.89

Isoelectric Point (pI)

27.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 3 - 143 7.2e-26 CobW/HypB/UreG, nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012327)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34470 AT2G34470
fragaria_vesca FvH4_3g05780
malus_domestica MD05G1285700.v1.1 MD10G1263700.v1.1
prunus_persica Prupe.4G079000_v2.0.a1
pyrus_communis pycom10g21990
rosa_chinensis RchiOBHm_Chr5g0013721 RchiOBHm_Chr5g0013731 RchiOBHm_Chr5g0013741 RchiOBHm_Chr5g0013751
rosa_laevigata RLG00000032043
rosa_multiflora Rmu_ssc0000256.1_g000001
rosa_roxburghii Rroxscaffold_1G00062890
rosa_rugosa Rorug05G0012600
rosa_samantha Rh5AG106900 Rh5BG103000 Rh5DG101700
rosa_wichuraiana Rw5G009320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 126
AciI CCGC 1 cut(s) 207
AcsI RAATTY 1 cut(s) 288
AcuI CTGAAG 2 cut(s) 93, 214
AfaI GTAC 1 cut(s) 176
AfiI CCNNNNNNNGG 2 cut(s) 157, 299
AgsI TTSAA 1 cut(s) 472
AjnI CCWGG 1 cut(s) 307
AloI GAACNNNNNNTCC 2 cut(s) 29, 61
AluBI AGCT 5 cut(s) 163, 221, 241, 323, 371
AluI AGCT 5 cut(s) 163, 221, 241, 323, 371
Alw21I GWGCWC 1 cut(s) 67
AoxI GGCC 3 cut(s) 148, 304, 419
ApeKI GCWGC 2 cut(s) 116, 221
ApoI RAATTY 1 cut(s) 288
AseI ATTAAT 1 cut(s) 141
AspLEI GCGC 1 cut(s) 516
AspS9I GGNCC 3 cut(s) 149, 305, 419
AsuHPI GGTGA 6 cut(s) 19, 53, 64, 293, 346, 451
AsuII TTCGAA 1 cut(s) 403
BarI GAAGNNNNNNTAC 2 cut(s) 66, 98
BbsI GAAGAC 1 cut(s) 135
Bbv12I GWGCWC 1 cut(s) 67
BbvI GCAGC 2 cut(s) 103, 208
BccI CCATC 3 cut(s) 32, 379, 407
BciT130I CCWGG 1 cut(s) 309
BfaI CTAG 3 cut(s) 8, 146, 497
BfoI RGCGCY 1 cut(s) 517
BisI GCNGC 2 cut(s) 117, 222
BlsI GCNGC 2 cut(s) 118, 223
Bme1390I CCNGG 1 cut(s) 309
BmgT120I GGNCC 3 cut(s) 149, 305, 419
BmrFI CCNGG 1 cut(s) 309
BmrI ACTGGG 1 cut(s) 108
BmsI GCATC 1 cut(s) 95
BmuI ACTGGG 1 cut(s) 108
BpiI GAAGAC 1 cut(s) 135
Bpu10I CCTNAGC 1 cut(s) 435
Bpu14I TTCGAA 1 cut(s) 403
BpuEI CTTGAG 2 cut(s) 176, 303
BsaAI YACGTR 1 cut(s) 86
BsaJI CCNNGG 1 cut(s) 307
Bsc4I CCNNNNNNNGG 2 cut(s) 157, 299
Bse1I ACTGG 1 cut(s) 103
BseBI CCWGG 1 cut(s) 309
BseDI CCNNGG 1 cut(s) 307
BseGI GGATG 2 cut(s) 43, 110
BseLI CCNNNNNNNGG 2 cut(s) 157, 299
BseMII CTCAG 1 cut(s) 449
BseNI ACTGG 1 cut(s) 103
BseRI GAGGAG 1 cut(s) 173
BseXI GCAGC 2 cut(s) 103, 208
Bsh1236I CGCG 1 cut(s) 126
BshFI GGCC 3 cut(s) 150, 306, 421
BsiHKAI GWGCWC 1 cut(s) 67
BslI CCNNNNNNNGG 2 cut(s) 157, 299
BsmI GAATGC 1 cut(s) 411
BsnI GGCC 3 cut(s) 150, 306, 421
Bsp119I TTCGAA 1 cut(s) 403
Bsp1286I GDGCHC 1 cut(s) 67
Bsp1407I TGTACA 1 cut(s) 174
Bsp68I TCGCGA 1 cut(s) 126
BspACI CCGC 1 cut(s) 207
BspANI GGCC 3 cut(s) 150, 306, 421
BspCNI CTCAG 1 cut(s) 448
BspFNI CGCG 1 cut(s) 126
BspT104I TTCGAA 1 cut(s) 403
BsrGI TGTACA 1 cut(s) 174
BsrI ACTGG 1 cut(s) 103
BssECI CCNNGG 1 cut(s) 307
Bst2UI CCWGG 1 cut(s) 309
Bst6I CTCTTC 1 cut(s) 69
BstAUI TGTACA 1 cut(s) 174
BstBAI YACGTR 1 cut(s) 86
BstBI TTCGAA 1 cut(s) 403
BstC8I GCNNGC 1 cut(s) 243
BstDEI CTNAG 1 cut(s) 435
BstF5I GGATG 2 cut(s) 43, 110
BstFNI CGCG 1 cut(s) 126
BstH2I RGCGCY 1 cut(s) 517
BstHHI GCGC 1 cut(s) 516
BstMWI GCNNNNNNNGC 2 cut(s) 368, 377
BstNI CCWGG 1 cut(s) 309
BstSCI CCNGG 1 cut(s) 307
BstUI CGCG 1 cut(s) 126
BstV1I GCAGC 2 cut(s) 103, 208
BstV2I GAAGAC 1 cut(s) 135
BsuRI GGCC 3 cut(s) 150, 306, 421
BtsCI GGATG 2 cut(s) 43, 110
BtsI GCAGTG 1 cut(s) 387
BtsIMutI CAGTG 2 cut(s) 387, 517
BtuMI TCGCGA 1 cut(s) 126
Cac8I GCNNGC 1 cut(s) 243
CfoI GCGC 1 cut(s) 516
Cfr13I GGNCC 3 cut(s) 149, 305, 419
CseI GACGC 1 cut(s) 404
Csp6I GTAC 1 cut(s) 175
CviAII CATG 2 cut(s) 59, 113
CviQI GTAC 1 cut(s) 175
DdeI CTNAG 1 cut(s) 435
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
EciI GGCGGA 1 cut(s) 222
Eco57I CTGAAG 2 cut(s) 93, 214
EcoO109I RGGNCCY 1 cut(s) 149
EcoRII CCWGG 1 cut(s) 307
FaeI CATG 2 cut(s) 62, 116
FaiI YATR 7 cut(s) 23, 60, 114, 188, 252, 266, 479
FalI AAGNNNNNCTT 2 cut(s) 312, 344
FatI CATG 2 cut(s) 58, 112
Fnu4HI GCNGC 2 cut(s) 117, 222
FokI GGATG 2 cut(s) 50, 117
Fsp4HI GCNGC 2 cut(s) 117, 222
FspBI CTAG 3 cut(s) 8, 146, 497
GlaI GCGC 1 cut(s) 515
GluI GCNGC 2 cut(s) 117, 222
HaeII RGCGCY 1 cut(s) 517
HaeIII GGCC 3 cut(s) 150, 306, 421
HgaI GACGC 1 cut(s) 404
HhaI GCGC 1 cut(s) 516
Hin1II CATG 2 cut(s) 62, 116
Hin6I GCGC 1 cut(s) 514
HinP1I GCGC 1 cut(s) 514
HinfI GANTC 2 cut(s) 200, 312
HphI GGTGA 6 cut(s) 19, 53, 64, 293, 346, 451
Hpy188III TCNNGA 4 cut(s) 71, 125, 155, 469
HpyAV CCTTC 1 cut(s) 338
HpyCH4IV ACGT 1 cut(s) 85
HpyCH4V TGCA 2 cut(s) 119, 356
HpyF10VI GCNNNNNNNGC 2 cut(s) 368, 377
HpyF3I CTNAG 1 cut(s) 435
HpySE526I ACGT 1 cut(s) 85
Hsp92II CATG 2 cut(s) 62, 116
HspAI GCGC 1 cut(s) 514
LmnI GCTCC 5 cut(s) 62, 160, 238, 368, 388
Lsp1109I GCAGC 2 cut(s) 103, 208
LweI GCATC 1 cut(s) 95
MaeI CTAG 3 cut(s) 8, 146, 497
MaeII ACGT 1 cut(s) 85
MaeIII GTNAC 2 cut(s) 10, 392
MboII GAAGA 2 cut(s) 86, 140
MhlI GDGCHC 1 cut(s) 67
MluCI AATT 3 cut(s) 120, 214, 288
MmeI TCCRAC 1 cut(s) 346
MnlI CCTC 6 cut(s) 28, 120, 151, 162, 410, 454
MseI TTAA 1 cut(s) 141
MspR9I CCNGG 1 cut(s) 309
Mva1269I GAATGC 1 cut(s) 411
MvaI CCWGG 1 cut(s) 309
MvnI CGCG 1 cut(s) 126
MwoI GCNNNNNNNGC 2 cut(s) 368, 377
NlaIII CATG 2 cut(s) 62, 116
NmuCI GTSAC 2 cut(s) 10, 392
NruI TCGCGA 1 cut(s) 126
NspV TTCGAA 1 cut(s) 403
PctI GAATGC 1 cut(s) 411
PfeI GAWTC 2 cut(s) 200, 312
PkrI GCNGC 2 cut(s) 118, 223
Ppu21I YACGTR 1 cut(s) 86
PshBI ATTAAT 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 307
PspGI CCWGG 1 cut(s) 307
PspPI GGNCC 3 cut(s) 149, 305, 419
RruI TCGCGA 1 cut(s) 126
RsaI GTAC 1 cut(s) 176
RsaNI GTAC 1 cut(s) 175
SaqAI TTAA 1 cut(s) 141
SatI GCNGC 2 cut(s) 117, 222
Sau96I GGNCC 3 cut(s) 149, 305, 419
ScrFI CCNGG 1 cut(s) 309
SduI GDGCHC 1 cut(s) 67
SfaNI GCATC 1 cut(s) 95
SfuI TTCGAA 1 cut(s) 403
SmlI CTYRAG 2 cut(s) 155, 318
SmoI CTYRAG 2 cut(s) 155, 318
Sse9I AATT 3 cut(s) 120, 214, 288
SsiI CCGC 1 cut(s) 207
SspMI CTAG 3 cut(s) 8, 146, 497
StyD4I CCNGG 1 cut(s) 307
TaiI ACGT 1 cut(s) 88
TaqI TCGA 1 cut(s) 403
TasI AATT 3 cut(s) 120, 214, 288
TatI WGTACW 1 cut(s) 174
TfiI GAWTC 2 cut(s) 200, 312
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TscAI CASTG 1 cut(s) 387
TseFI GTSAC 2 cut(s) 10, 392
TseI GCWGC 2 cut(s) 116, 221
Tsp45I GTSAC 2 cut(s) 10, 392
TspRI CASTG 1 cut(s) 387
VspI ATTAAT 1 cut(s) 141
XapI RAATTY 1 cut(s) 288
XspI CTAG 3 cut(s) 8, 146, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.