Rh5BG103000

urease accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0012327
Physical Location & Seq
Reverse (-)
9425172 .. 9427530
2359 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG103000.1

Sequence Viewer

Length: 837 bp
ATGGCAGAACACCATCATCATGACCATGATCATCATCACCATCATGATCATGATCATGATCACAGCCATGGGGATTCAGCAGCAACAAAGTCATGGACAGGAGCAGATGGGAAGGTGTACCATAGCCATGATGGGCTGGCACCACACTCACATGAACCCATCTACTCTCCTGGTTACTTCAGCAGAAGAGCTCCACCACTCCTCTCCAGGGATTTCAAGGAAAGGGCTTTCACTATTGGCATTGGAGGACCGGTTGGTACTGGGAAAACTGCTTTGATGTTGGCTCTGTGTAAACTTTTGCGCGACAAGTACAGTCTTGCAGCAGTGACAAATGATATATTCACCAAAGAGGATGGTGAGTTCTTGGTGAAGCATGGAGCACTTCCTGAAGAGAGAATACGTGCTGTGGAAACTGGGGGATGCCCTCATGCTGCAATTCGCGAAGACATCAGCATTAATCTAGGCCCTCTTGAGGAGCTTTCTAACTTGTACAAGACAGACATACTTCTTTGTGAATCAGGCGGAGATAATTTAGCTGCGAACTTCAGCAGGGAGCTGGCTGACTATATTATTTACATCATAGATGTATCTGGTGGTGATAAAATTCCACGAAAAGGTGGCCCTGGAATCACTCAAGCTGACCTTCTGGTGATAAACAAGACTGACCTTGCACAAGCAGTTGGAGCTGATTTGGCAGTGATGGAGCGTGACGCACTTCGAATGCGAGATGGAGGGCCATTTGTCTTTGCTCAGGTGAAGCACGGGCAAGGAGTTGAGGAAATCGTGAACCACATATTACAGGCTTGGGAACTAGCAACAGGAAATAAGCGCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006807 GO:0008150 GO:0008152 GO:0043085 GO:0044093 GO:0050790 GO:0065007 GO:0065009
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cobW

Protein Analysis

278

Amino Acids

30.46

Weight (kDa)

5.99

Isoelectric Point (pI)

27.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 78 - 248 1.2e-35 CobW/HypB/UreG, nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012327)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34470 AT2G34470
fragaria_vesca FvH4_3g05780
malus_domestica MD05G1285700.v1.1 MD10G1263700.v1.1
prunus_persica Prupe.4G079000_v2.0.a1
pyrus_communis pycom10g21990
rosa_chinensis RchiOBHm_Chr5g0013721 RchiOBHm_Chr5g0013731 RchiOBHm_Chr5g0013741 RchiOBHm_Chr5g0013751
rosa_laevigata RLG00000032043
rosa_multiflora Rmu_ssc0000256.1_g000001
rosa_roxburghii Rroxscaffold_1G00062890
rosa_rugosa Rorug05G0012600
rosa_samantha Rh5AG106900 Rh5BG103000 Rh5DG101700
rosa_wichuraiana Rw5G009320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 139
AccII CGCG 2 cut(s) 303, 441
AciI CCGC 1 cut(s) 522
AcsI RAATTY 1 cut(s) 603
AcuI CTGAAG 3 cut(s) 163, 408, 529
AfaI GTAC 4 cut(s) 119, 259, 311, 491
AfiI CCNNNNNNNGG 3 cut(s) 208, 472, 614
AgeI ACCGGT 1 cut(s) 250
AgsI TTSAA 1 cut(s) 217
AjnI CCWGG 3 cut(s) 169, 206, 622
AloI GAACNNNNNNTCC 2 cut(s) 344, 376
AluBI AGCT 6 cut(s) 191, 478, 536, 556, 638, 686
AluI AGCT 6 cut(s) 191, 478, 536, 556, 638, 686
Alw21I GWGCWC 2 cut(s) 193, 382
AoxI GGCC 3 cut(s) 463, 619, 734
ApeKI GCWGC 4 cut(s) 80, 320, 431, 536
ApoI RAATTY 1 cut(s) 603
AseI ATTAAT 1 cut(s) 456
AsiGI ACCGGT 1 cut(s) 250
AspLEI GCGC 2 cut(s) 303, 831
AspS9I GGNCC 4 cut(s) 248, 464, 620, 734
AsuHPI GGTGA 7 cut(s) 29, 334, 368, 379, 608, 661, 766
AsuII TTCGAA 1 cut(s) 718
AvaII GGWCC 1 cut(s) 248
BanI GGYRCC 1 cut(s) 139
BanII GRGCYC 1 cut(s) 193
BarI GAAGNNNNNNTAC 2 cut(s) 381, 413
BbsI GAAGAC 1 cut(s) 450
Bbv12I GWGCWC 2 cut(s) 193, 382
BbvI GCAGC 4 cut(s) 92, 332, 418, 523
BccI CCATC 8 cut(s) 21, 48, 101, 125, 167, 347, 694, 722
BciT130I CCWGG 3 cut(s) 171, 208, 624
BclI TGATCA 4 cut(s) 28, 46, 52, 58
BfaI CTAG 2 cut(s) 461, 812
BfoI RGCGCY 1 cut(s) 832
BisI GCNGC 4 cut(s) 81, 321, 432, 537
BlsI GCNGC 4 cut(s) 82, 322, 433, 538
Bme1390I CCNGG 3 cut(s) 171, 208, 624
Bme18I GGWCC 1 cut(s) 248
BmgT120I GGNCC 4 cut(s) 248, 464, 620, 734
BmiI GGNNCC 1 cut(s) 141
BmrFI CCNGG 3 cut(s) 171, 208, 624
BmrI ACTGGG 2 cut(s) 270, 423
BmsI GCATC 1 cut(s) 410
BmuI ACTGGG 2 cut(s) 270, 423
BpiI GAAGAC 1 cut(s) 450
BpmI CTGGAG 1 cut(s) 190
Bpu10I CCTNAGC 1 cut(s) 750
Bpu14I TTCGAA 1 cut(s) 718
BpuEI CTTGAG 2 cut(s) 491, 618
BsaAI YACGTR 1 cut(s) 401
BsaBI GATNNNNATC 3 cut(s) 33, 51, 57
BsaJI CCNNGG 3 cut(s) 67, 207, 622
BsaWI WCCGGW 1 cut(s) 250
BsaXI ACNNNNNCTCC 2 cut(s) 237, 267
Bsc4I CCNNNNNNNGG 3 cut(s) 208, 472, 614
Bse118I RCCGGY 1 cut(s) 250
Bse1I ACTGG 2 cut(s) 265, 418
Bse8I GATNNNNATC 3 cut(s) 33, 51, 57
BseBI CCWGG 3 cut(s) 171, 208, 624
BseDI CCNNGG 3 cut(s) 67, 207, 622
BseGI GGATG 2 cut(s) 358, 425
BseJI GATNNNNATC 3 cut(s) 33, 51, 57
BseLI CCNNNNNNNGG 3 cut(s) 208, 472, 614
BseMII CTCAG 1 cut(s) 764
BseNI ACTGG 2 cut(s) 265, 418
BseRI GAGGAG 2 cut(s) 191, 488
BseXI GCAGC 4 cut(s) 92, 332, 418, 523
Bsh1236I CGCG 2 cut(s) 303, 441
BshFI GGCC 3 cut(s) 465, 621, 736
BshNI GGYRCC 1 cut(s) 139
BshTI ACCGGT 1 cut(s) 250
BsiHKAI GWGCWC 2 cut(s) 193, 382
BsiSI CCGG 1 cut(s) 251
BslI CCNNNNNNNGG 3 cut(s) 208, 472, 614
BsmI GAATGC 1 cut(s) 726
BsnI GGCC 3 cut(s) 465, 621, 736
Bsp119I TTCGAA 1 cut(s) 718
Bsp1286I GDGCHC 2 cut(s) 193, 382
Bsp1407I TGTACA 1 cut(s) 489
Bsp143I GATC 4 cut(s) 28, 46, 52, 58
Bsp19I CCATGG 1 cut(s) 67
Bsp68I TCGCGA 1 cut(s) 441
BspACI CCGC 1 cut(s) 522
BspANI GGCC 3 cut(s) 465, 621, 736
BspCNI CTCAG 1 cut(s) 763
BspFNI CGCG 2 cut(s) 303, 441
BspHI TCATGA 4 cut(s) 19, 43, 49, 55
BspLI GGNNCC 1 cut(s) 141
BspQI GCTCTTC 1 cut(s) 181
BspT104I TTCGAA 1 cut(s) 718
BspT107I GGYRCC 1 cut(s) 139
BsrFI RCCGGY 1 cut(s) 250
BsrGI TGTACA 1 cut(s) 489
BsrI ACTGG 2 cut(s) 265, 418
BssAI RCCGGY 1 cut(s) 250
BssECI CCNNGG 3 cut(s) 67, 207, 622
BssMI GATC 4 cut(s) 28, 46, 52, 58
BssT1I CCWWGG 1 cut(s) 67
Bst2UI CCWGG 3 cut(s) 171, 208, 624
Bst4CI ACNGT 1 cut(s) 314
Bst6I CTCTTC 2 cut(s) 181, 384
BstAUI TGTACA 1 cut(s) 489
BstBAI YACGTR 1 cut(s) 401
BstBI TTCGAA 1 cut(s) 718
BstC8I GCNNGC 2 cut(s) 138, 558
BstDEI CTNAG 1 cut(s) 750
BstDSI CCRYGG 1 cut(s) 67
BstF5I GGATG 2 cut(s) 358, 425
BstFNI CGCG 2 cut(s) 303, 441
BstH2I RGCGCY 1 cut(s) 832
BstHHI GCGC 2 cut(s) 303, 831
BstKTI GATC 4 cut(s) 31, 49, 55, 61
BstMBI GATC 4 cut(s) 28, 46, 52, 58
BstMWI GCNNNNNNNGC 2 cut(s) 683, 692
BstNI CCWGG 3 cut(s) 171, 208, 624
BstSCI CCNGG 3 cut(s) 169, 206, 622
BstUI CGCG 2 cut(s) 303, 441
BstV1I GCAGC 4 cut(s) 92, 332, 418, 523
BstV2I GAAGAC 1 cut(s) 450
BsuRI GGCC 3 cut(s) 465, 621, 736
BtgI CCRYGG 1 cut(s) 67
BtsCI GGATG 2 cut(s) 358, 425
BtsI GCAGTG 2 cut(s) 330, 702
BtsIMutI CAGTG 3 cut(s) 330, 702, 832
BtuMI TCGCGA 1 cut(s) 441
Cac8I GCNNGC 2 cut(s) 138, 558
CciI TCATGA 4 cut(s) 19, 43, 49, 55
CfoI GCGC 2 cut(s) 303, 831
Cfr10I RCCGGY 1 cut(s) 250
Cfr13I GGNCC 4 cut(s) 248, 464, 620, 734
CseI GACGC 1 cut(s) 719
Csp6I GTAC 4 cut(s) 118, 258, 310, 490
CspAI ACCGGT 1 cut(s) 250
CviQI GTAC 4 cut(s) 118, 258, 310, 490
DdeI CTNAG 1 cut(s) 750
DpnI GATC 4 cut(s) 30, 48, 54, 60
DpnII GATC 4 cut(s) 28, 46, 52, 58
Eam1104I CTCTTC 2 cut(s) 181, 384
EarI CTCTTC 2 cut(s) 181, 384
EciI GGCGGA 1 cut(s) 537
Ecl136II GAGCTC 1 cut(s) 191
Eco130I CCWWGG 1 cut(s) 67
Eco24I GRGCYC 1 cut(s) 193
Eco47I GGWCC 1 cut(s) 248
Eco53kI GAGCTC 1 cut(s) 191
Eco57I CTGAAG 3 cut(s) 163, 408, 529
EcoICRI GAGCTC 1 cut(s) 191
EcoO109I RGGNCCY 1 cut(s) 464
EcoRII CCWGG 3 cut(s) 169, 206, 622
EcoT14I CCWWGG 1 cut(s) 67
EcoT38I GRGCYC 1 cut(s) 193
ErhI CCWWGG 1 cut(s) 67
FalI AAGNNNNNCTT 2 cut(s) 627, 659
FbaI TGATCA 4 cut(s) 28, 46, 52, 58
Fnu4HI GCNGC 4 cut(s) 81, 321, 432, 537
FokI GGATG 2 cut(s) 365, 432
FriOI GRGCYC 1 cut(s) 193
Fsp4HI GCNGC 4 cut(s) 81, 321, 432, 537
FspBI CTAG 2 cut(s) 461, 812
GlaI GCGC 2 cut(s) 302, 830
GluI GCNGC 4 cut(s) 81, 321, 432, 537
GsuI CTGGAG 1 cut(s) 190
HaeII RGCGCY 1 cut(s) 832
HaeIII GGCC 3 cut(s) 465, 621, 736
HapII CCGG 1 cut(s) 251
HgaI GACGC 1 cut(s) 719
HhaI GCGC 2 cut(s) 303, 831
Hin6I GCGC 2 cut(s) 301, 829
HinP1I GCGC 2 cut(s) 301, 829
HinfI GANTC 3 cut(s) 74, 515, 627
HpaII CCGG 1 cut(s) 251
HphI GGTGA 7 cut(s) 29, 334, 368, 379, 608, 661, 766
Hpy166II GTNNAC 3 cut(s) 118, 293, 787
Hpy188III TCNNGA 8 cut(s) 20, 44, 50, 56, 386, 440, 470, 784
Hpy8I GTNNAC 3 cut(s) 118, 293, 787
HpyAV CCTTC 2 cut(s) 106, 653
HpyCH4III ACNGT 1 cut(s) 314
HpyCH4IV ACGT 1 cut(s) 400
HpyCH4V TGCA 3 cut(s) 320, 434, 671
HpyF10VI GCNNNNNNNGC 2 cut(s) 683, 692
HpyF3I CTNAG 1 cut(s) 750
HpySE526I ACGT 1 cut(s) 400
HspAI GCGC 2 cut(s) 301, 829
Ksp22I TGATCA 4 cut(s) 28, 46, 52, 58
Kzo9I GATC 4 cut(s) 28, 46, 52, 58
LguI GCTCTTC 1 cut(s) 181
LmnI GCTCC 7 cut(s) 101, 196, 377, 475, 553, 683, 703
Lsp1109I GCAGC 4 cut(s) 92, 332, 418, 523
LweI GCATC 1 cut(s) 410
MaeI CTAG 2 cut(s) 461, 812
MaeII ACGT 1 cut(s) 400
MaeIII GTNAC 3 cut(s) 173, 325, 707
MalI GATC 4 cut(s) 30, 48, 54, 60
MboI GATC 4 cut(s) 28, 46, 52, 58
MboII GAAGA 3 cut(s) 198, 401, 455
MhlI GDGCHC 2 cut(s) 193, 382
MluCI AATT 3 cut(s) 435, 529, 603
MmeI TCCRAC 1 cut(s) 661
MnlI CCTC 8 cut(s) 212, 239, 343, 435, 466, 477, 725, 769
MseI TTAA 1 cut(s) 456
MslI CAYNNNNRTG 8 cut(s) 18, 24, 42, 48, 54, 66, 126, 150
MspI CCGG 1 cut(s) 251
MspR9I CCNGG 3 cut(s) 171, 208, 624
Mva1269I GAATGC 1 cut(s) 726
MvaI CCWGG 3 cut(s) 171, 208, 624
MvnI CGCG 2 cut(s) 303, 441
MwoI GCNNNNNNNGC 2 cut(s) 683, 692
NcoI CCATGG 1 cut(s) 67
NdeII GATC 4 cut(s) 28, 46, 52, 58
NlaIV GGNNCC 1 cut(s) 141
NmuCI GTSAC 2 cut(s) 325, 707
NruI TCGCGA 1 cut(s) 441
NspV TTCGAA 1 cut(s) 718
PagI TCATGA 4 cut(s) 19, 43, 49, 55
PciSI GCTCTTC 1 cut(s) 181
PctI GAATGC 1 cut(s) 726
PfeI GAWTC 3 cut(s) 74, 515, 627
PinAI ACCGGT 1 cut(s) 250
PkrI GCNGC 4 cut(s) 82, 322, 433, 538
Ppu21I YACGTR 1 cut(s) 401
PshBI ATTAAT 1 cut(s) 456
Psp124BI GAGCTC 1 cut(s) 193
Psp6I CCWGG 3 cut(s) 169, 206, 622
PspGI CCWGG 3 cut(s) 169, 206, 622
PspN4I GGNNCC 1 cut(s) 141
PspPI GGNCC 4 cut(s) 248, 464, 620, 734
RruI TCGCGA 1 cut(s) 441
RsaI GTAC 4 cut(s) 119, 259, 311, 491
RsaNI GTAC 4 cut(s) 118, 258, 310, 490
RseI CAYNNNNRTG 8 cut(s) 18, 24, 42, 48, 54, 66, 126, 150
SacI GAGCTC 1 cut(s) 193
SapI GCTCTTC 1 cut(s) 181
SaqAI TTAA 1 cut(s) 456
SatI GCNGC 4 cut(s) 81, 321, 432, 537
Sau3AI GATC 4 cut(s) 28, 46, 52, 58
Sau96I GGNCC 4 cut(s) 248, 464, 620, 734
ScrFI CCNGG 3 cut(s) 171, 208, 624
SduI GDGCHC 2 cut(s) 193, 382
SfaNI GCATC 1 cut(s) 410
SfuI TTCGAA 1 cut(s) 718
SinI GGWCC 1 cut(s) 248
SmiMI CAYNNNNRTG 8 cut(s) 18, 24, 42, 48, 54, 66, 126, 150
SmlI CTYRAG 2 cut(s) 470, 633
SmoI CTYRAG 2 cut(s) 470, 633
Sse9I AATT 3 cut(s) 435, 529, 603
SsiI CCGC 1 cut(s) 522
SspMI CTAG 2 cut(s) 461, 812
SstI GAGCTC 1 cut(s) 193
StyD4I CCNGG 3 cut(s) 169, 206, 622
StyI CCWWGG 1 cut(s) 67
TaaI ACNGT 1 cut(s) 314
TaiI ACGT 1 cut(s) 403
TaqI TCGA 1 cut(s) 718
TasI AATT 3 cut(s) 435, 529, 603
TatI WGTACW 2 cut(s) 309, 489
TfiI GAWTC 3 cut(s) 74, 515, 627
Tru1I TTAA 1 cut(s) 456
Tru9I TTAA 1 cut(s) 456
TscAI CASTG 2 cut(s) 330, 702
TseFI GTSAC 2 cut(s) 325, 707
TseI GCWGC 4 cut(s) 80, 320, 431, 536
Tsp45I GTSAC 2 cut(s) 325, 707
TspDTI ATGAA 1 cut(s) 168
TspRI CASTG 2 cut(s) 330, 702
VpaK11BI GGWCC 1 cut(s) 248
VspI ATTAAT 1 cut(s) 456
XapI RAATTY 1 cut(s) 603
XcmI CCANNNNNNNNNTGG 1 cut(s) 128
XspI CTAG 2 cut(s) 461, 812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.