MD05G1290500.v1.1

DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007782
Physical Location & Seq
Reverse (-)
42230292 .. 42232850
2559 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1290500.v1.1.491

Sequence Viewer

Length: 399 bp
ATGGCTGATCCTGAACTGGAAGCAATCAGACAAAGAAGAATGCAGGAGCTTATGGCTCAACATGGTGTAGGAGGGTCTGGAGGGCAACAACAAAACCCAGAGCAGCACAAGGCAAATGAAGATGCCAAGAGGGAGGCCGAAGAACGGAGGCAGATGATGCTTAGTCAGATTGTGTCAGGCGAAGCTCGTGAAAGAATTGCTAGAATTGCTTTGGTGAAACCTGAGAAGGCAAGGGGTGTTGAAGATGTTATACTGAGAGCTGCTCAAATGGGCCAGATAGTTGAGAAGGTATCTGAGGAGAAACTCATTTCGTTGTTGGAGCAGATCAACAACCAAACAAACAAGCAGACCAAAGTCACTATTCAGAGGCGTCGGAGCGTCCTTGAGGACGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005539 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006915 GO:0008047 GO:0008092 GO:0008150 GO:0008201 GO:0008219 GO:0008285 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010604 GO:0010628 GO:0010638 GO:0010646 GO:0010647 GO:0010698 GO:0010821 GO:0010822 GO:0010941 GO:0010942 GO:0010950 GO:0010952 GO:0012501 GO:0015631 GO:0019222 GO:0023051 GO:0023056 GO:0030162 GO:0030234 GO:0031323 GO:0031325 GO:0032268 GO:0032270 GO:0032386 GO:0032388 GO:0032879 GO:0032880 GO:0033043 GO:0033157 GO:0042127 GO:0042221 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043280 GO:0043281 GO:0044093 GO:0044424 GO:0044444 GO:0044464 GO:0045862 GO:0048487 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051222 GO:0051223 GO:0051246 GO:0051247 GO:0051336 GO:0051345 GO:0051716 GO:0052547 GO:0052548 GO:0060255 GO:0060341 GO:0065007 GO:0065009 GO:0070201 GO:0070848 GO:0070887 GO:0071310 GO:0071363 GO:0071495 GO:0071559 GO:0071560 GO:0080090 GO:0090087 GO:0090199 GO:0090200 GO:0090316 GO:0097367 GO:0098772 GO:1901681 GO:1903214 GO:1903332 GO:1903333 GO:1903533 GO:1903636 GO:1903638 GO:1903644 GO:1903645 GO:1903747 GO:1903749 GO:1903827 GO:1903829 GO:1903955 GO:1904589 GO:1904591 GO:1904951 GO:1905475 GO:1905477 GO:2000116 GO:2001056 GO:2001233 GO:2001235
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsDNA_bind

Protein Analysis

133

Amino Acids

15.02

Weight (kDa)

6.19

Isoelectric Point (pI)

60.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
dsDNA_bind PF01984 9 - 124 7.1e-30 Double-stranded DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 2
AcyI GRCGYC 1 cut(s) 370
AfiI CCNNNNNNNGG 1 cut(s) 144
AgsI TTSAA 1 cut(s) 242
AluBI AGCT 3 cut(s) 49, 185, 260
AluI AGCT 3 cut(s) 49, 185, 260
AlwI GGATC 1 cut(s) 2
AoxI GGCC 2 cut(s) 135, 271
ApeKI GCWGC 2 cut(s) 103, 260
AspS9I GGNCC 1 cut(s) 271
AsuHPI GGTGA 1 cut(s) 226
BarI GAAGNNNNNNTAC 2 cut(s) 234, 266
BauI CACGAG 1 cut(s) 186
BbvI GCAGC 2 cut(s) 115, 247
BfaI CTAG 1 cut(s) 201
BisI GCNGC 2 cut(s) 104, 261
BlsI GCNGC 2 cut(s) 105, 262
BmgT120I GGNCC 1 cut(s) 271
BmsI GCATC 2 cut(s) 112, 147
BoxI GACNNNNGTC 1 cut(s) 353
BplI GAGNNNNNCTC 2 cut(s) 247, 279
BpmI CTGGAG 1 cut(s) 99
BsaHI GRCGYC 1 cut(s) 370
Bsc4I CCNNNNNNNGG 1 cut(s) 144
Bse1I ACTGG 1 cut(s) 21
BseLI CCNNNNNNNGG 1 cut(s) 144
BseMII CTCAG 3 cut(s) 213, 245, 285
BseNI ACTGG 1 cut(s) 21
BseRI GAGGAG 1 cut(s) 311
BseXI GCAGC 2 cut(s) 115, 247
BshFI GGCC 2 cut(s) 137, 273
BslI CCNNNNNNNGG 1 cut(s) 144
BsmI GAATGC 1 cut(s) 45
BsnI GGCC 2 cut(s) 137, 273
Bsp143I GATC 2 cut(s) 7, 324
BspANI GGCC 2 cut(s) 137, 273
BspCNI CTCAG 3 cut(s) 214, 246, 286
BspPI GGATC 1 cut(s) 2
BsrI ACTGG 1 cut(s) 21
BssMI GATC 2 cut(s) 7, 324
BssNI GRCGYC 1 cut(s) 370
BssSI CACGAG 1 cut(s) 186
Bst2BI CACGAG 1 cut(s) 186
BstACI GRCGYC 1 cut(s) 370
BstAPI GCANNNNNTGC 1 cut(s) 157
BstDEI CTNAG 4 cut(s) 161, 222, 254, 294
BstKTI GATC 2 cut(s) 10, 327
BstMBI GATC 2 cut(s) 7, 324
BstMWI GCNNNNNNNGC 2 cut(s) 157, 206
BstPAI GACNNNNGTC 1 cut(s) 353
BstV1I GCAGC 2 cut(s) 115, 247
BsuRI GGCC 2 cut(s) 137, 273
Cfr13I GGNCC 1 cut(s) 271
CseI GACGC 2 cut(s) 359, 367
CviAII CATG 1 cut(s) 62
CviJI RGCY 7 cut(s) 5, 49, 56, 137, 185, 260, 273
CviKI_1 RGCY 7 cut(s) 5, 49, 56, 137, 185, 260, 273
DdeI CTNAG 4 cut(s) 161, 222, 254, 294
DpnI GATC 2 cut(s) 9, 326
DpnII GATC 2 cut(s) 7, 324
FaeI CATG 1 cut(s) 65
FaiI YATR 3 cut(s) 53, 63, 251
FatI CATG 1 cut(s) 61
Fnu4HI GCNGC 2 cut(s) 104, 261
Fsp4HI GCNGC 2 cut(s) 104, 261
FspBI CTAG 1 cut(s) 201
GluI GCNGC 2 cut(s) 104, 261
GsuI CTGGAG 1 cut(s) 99
HaeIII GGCC 2 cut(s) 137, 273
HgaI GACGC 2 cut(s) 359, 367
Hin1I GRCGYC 1 cut(s) 370
Hin1II CATG 1 cut(s) 65
HphI GGTGA 1 cut(s) 226
Hpy188I TCNGA 5 cut(s) 29, 168, 295, 366, 375
Hpy188III TCNNGA 3 cut(s) 11, 78, 188
Hpy99I CGWCG 1 cut(s) 375
HpyAV CCTTC 2 cut(s) 220, 280
HpyCH4V TGCA 1 cut(s) 43
HpyF10VI GCNNNNNNNGC 2 cut(s) 157, 206
HpyF3I CTNAG 4 cut(s) 161, 222, 254, 294
Hsp92I GRCGYC 1 cut(s) 370
Hsp92II CATG 1 cut(s) 65
Kzo9I GATC 2 cut(s) 7, 324
LmnI GCTCC 3 cut(s) 46, 319, 375
LpnPI CCDG 8 cut(s) 2, 24, 29, 63, 111, 162, 234, 287
Lsp1109I GCAGC 2 cut(s) 115, 247
LweI GCATC 2 cut(s) 112, 147
MaeI CTAG 1 cut(s) 201
MaeIII GTNAC 1 cut(s) 355
MalI GATC 2 cut(s) 9, 326
MboI GATC 2 cut(s) 7, 324
MboII GAAGA 4 cut(s) 48, 131, 152, 254
MluCI AATT 2 cut(s) 195, 204
MmeI TCCRAC 2 cut(s) 297, 353
MnlI CCTC 8 cut(s) 65, 74, 123, 127, 141, 289, 360, 379
Mva1269I GAATGC 1 cut(s) 45
MwoI GCNNNNNNNGC 2 cut(s) 157, 206
NdeII GATC 2 cut(s) 7, 324
NlaIII CATG 1 cut(s) 65
NmuCI GTSAC 1 cut(s) 355
PctI GAATGC 1 cut(s) 45
PkrI GCNGC 2 cut(s) 105, 262
PshAI GACNNNNGTC 1 cut(s) 353
PspPI GGNCC 1 cut(s) 271
SatI GCNGC 2 cut(s) 104, 261
Sau3AI GATC 2 cut(s) 7, 324
Sau96I GGNCC 1 cut(s) 271
SetI ASST 5 cut(s) 51, 187, 223, 262, 291
SfaNI GCATC 2 cut(s) 112, 147
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
Sse9I AATT 2 cut(s) 195, 204
SspMI CTAG 1 cut(s) 201
TasI AATT 2 cut(s) 195, 204
TseFI GTSAC 1 cut(s) 355
TseI GCWGC 2 cut(s) 103, 260
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 1 cut(s) 132
TspGWI ACGGA 1 cut(s) 160
XspI CTAG 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.