Rorug05G0007100

DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0007782
Physical Location & Seq
Forward (+)
414100 .. 415608
1509 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0007100.1

Sequence Viewer

Length: 618 bp
ATGAGCTACTTGGGTGTGGGTGTTAGTCCTGGGAATGTCCCAGTGTCTCACAGCAGCAGCACCACAAATCTAAAAGTGATCGATCGGAGAGTGAGGGTGGCAGAGTTGGTCTTGAGGTTTCTCATCTGTGGTCTCGGACTTGCCGCTGCTCTTCTTGTGGCCACTGATACTCAGGTCAAAGAGATCTTCATGATCAAGAAGAAGGCCAAGTTCACGGACATGAAGGCCCTTGTGTTTTTGGTGATAGTCAATGGAATAGCTGCTGCTTACTCTTTGCTGCAAGCACTGCGCTGTGTTATGAGCATGGTTAGAGGAAATGTTCTCTTCAGCAAGCCCTTAGCTTGGATTATATTCTCAGCTGACCAGGTGATGGCATATGTGGCTGTGGCTGCAATAGGAGCTGCAGCACAGTCTGCGGCCTTGGCCAAGTTGGGGCAGTCGGAGCTACAATGGATGAAGATCTGCAACGTGTATGACAAGTTCTGCAACCAAGTTGGGGAGGGAATTGCGAGCGCGGTGTTAGTTGGCCTCAGCACAATCCTCATCTCTTGCATTTCTGCTTTCAATCTCTTCCGATTGTATGGTGGCAACAAGAACAAGAGCAGCTCCAGGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005539 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006915 GO:0008047 GO:0008092 GO:0008150 GO:0008201 GO:0008219 GO:0008285 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010604 GO:0010628 GO:0010638 GO:0010646 GO:0010647 GO:0010698 GO:0010821 GO:0010822 GO:0010941 GO:0010942 GO:0010950 GO:0010952 GO:0012501 GO:0015631 GO:0019222 GO:0023051 GO:0023056 GO:0030162 GO:0030234 GO:0031323 GO:0031325 GO:0032268 GO:0032270 GO:0032386 GO:0032388 GO:0032879 GO:0032880 GO:0033043 GO:0033157 GO:0042127 GO:0042221 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043280 GO:0043281 GO:0044093 GO:0044424 GO:0044444 GO:0044464 GO:0045862 GO:0048487 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051222 GO:0051223 GO:0051246 GO:0051247 GO:0051336 GO:0051345 GO:0051716 GO:0052547 GO:0052548 GO:0060255 GO:0060341 GO:0065007 GO:0065009 GO:0070201 GO:0070848 GO:0070887 GO:0071310 GO:0071363 GO:0071495 GO:0071559 GO:0071560 GO:0080090 GO:0090087 GO:0090199 GO:0090200 GO:0090316 GO:0097367 GO:0098772 GO:1901681 GO:1903214 GO:1903332 GO:1903333 GO:1903533 GO:1903636 GO:1903638 GO:1903644 GO:1903645 GO:1903747 GO:1903749 GO:1903827 GO:1903829 GO:1903955 GO:1904589 GO:1904591 GO:1904951 GO:1905475 GO:1905477 GO:2000116 GO:2001056 GO:2001233 GO:2001235
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsDNA_bind

Protein Analysis

205

Amino Acids

22.0

Weight (kDa)

9.77

Isoelectric Point (pI)

34.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 29 - 174 1.7e-46 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 370
AccII CGCG 1 cut(s) 515
AciI CCGC 3 cut(s) 144, 416, 515
AcoI YGGCCR 2 cut(s) 159, 423
AcuI CTGAAG 1 cut(s) 310
AfiI CCNNNNNNNGG 4 cut(s) 342, 370, 432, 496
AflIII ACRYGT 1 cut(s) 468
AgsI TTSAA 1 cut(s) 565
AjnI CCWGG 3 cut(s) 28, 363, 608
AluBI AGCT 7 cut(s) 6, 260, 341, 359, 401, 445, 606
AluI AGCT 7 cut(s) 6, 260, 341, 359, 401, 445, 606
Alw26I GTCTC 2 cut(s) 51, 137
AoxI GGCC 6 cut(s) 159, 204, 225, 417, 423, 526
AspLEI GCGC 2 cut(s) 291, 515
AspS9I GGNCC 1 cut(s) 226
AsuHPI GGTGA 2 cut(s) 253, 379
BaeI ACNNNNGTAYC 2 cut(s) 159, 192
BalI TGGCCA 2 cut(s) 161, 425
BbvCI CCTCAGC 1 cut(s) 530
BbvI GCAGC 9 cut(s) 66, 69, 133, 247, 250, 264, 376, 388, 416
BccI CCATC 1 cut(s) 364
BciT130I CCWGG 3 cut(s) 30, 365, 610
BclI TGATCA 1 cut(s) 192
BcoDI GTCTC 2 cut(s) 51, 137
BfmI CTRYAG 1 cut(s) 402
BglII AGATCT 2 cut(s) 183, 459
Bme1390I CCNGG 3 cut(s) 30, 365, 610
BmgT120I GGNCC 1 cut(s) 226
BmrFI CCNGG 3 cut(s) 30, 365, 610
BmrI ACTGGG 1 cut(s) 35
BmuI ACTGGG 1 cut(s) 35
BpmI CTGGAG 1 cut(s) 592
Bpu10I CCTNAGC 2 cut(s) 337, 530
BpuEI CTTGAG 1 cut(s) 133
Bsa29I ATCGAT 1 cut(s) 81
BsaBI GATNNNNATC 1 cut(s) 458
BsaI GGTCTC 1 cut(s) 137
BsaJI CCNNGG 2 cut(s) 29, 420
Bsc4I CCNNNNNNNGG 4 cut(s) 342, 370, 432, 496
Bse1I ACTGG 1 cut(s) 41
Bse8I GATNNNNATC 1 cut(s) 458
BseBI CCWGG 3 cut(s) 30, 365, 610
BseCI ATCGAT 1 cut(s) 81
BseDI CCNNGG 2 cut(s) 29, 420
BseGI GGATG 1 cut(s) 459
BseJI GATNNNNATC 1 cut(s) 458
BseLI CCNNNNNNNGG 4 cut(s) 342, 370, 432, 496
BseMII CTCAG 3 cut(s) 185, 369, 544
BseNI ACTGG 1 cut(s) 41
BseXI GCAGC 9 cut(s) 66, 69, 133, 247, 250, 264, 376, 388, 416
Bsh1236I CGCG 1 cut(s) 515
Bsh1285I CGRYCG 1 cut(s) 85
BshFI GGCC 6 cut(s) 161, 206, 227, 419, 425, 528
BshVI ATCGAT 1 cut(s) 81
BsiEI CGRYCG 1 cut(s) 85
BslFI GGGAC 1 cut(s) 23
BslI CCNNNNNNNGG 4 cut(s) 342, 370, 432, 496
BsmAI GTCTC 2 cut(s) 51, 137
BsmFI GGGAC 1 cut(s) 23
BsnI GGCC 6 cut(s) 161, 206, 227, 419, 425, 528
Bso31I GGTCTC 1 cut(s) 137
Bsp143I GATC 5 cut(s) 78, 82, 183, 192, 459
BspACI CCGC 3 cut(s) 144, 416, 515
BspANI GGCC 6 cut(s) 161, 206, 227, 419, 425, 528
BspCNI CTCAG 3 cut(s) 184, 368, 543
BspDI ATCGAT 1 cut(s) 81
BspFNI CGCG 1 cut(s) 515
BspHI TCATGA 1 cut(s) 189
BspMAI CTGCAG 1 cut(s) 406
BspQI GCTCTTC 1 cut(s) 156
BspTNI GGTCTC 1 cut(s) 137
BsrI ACTGG 1 cut(s) 41
BssECI CCNNGG 2 cut(s) 29, 420
BssMI GATC 5 cut(s) 78, 82, 183, 192, 459
BssT1I CCWWGG 1 cut(s) 420
Bst2UI CCWGG 3 cut(s) 30, 365, 610
Bst4CI ACNGT 1 cut(s) 411
Bst6I CTCTTC 3 cut(s) 156, 329, 575
BstAPI GCANNNNNTGC 2 cut(s) 286, 413
BstC8I GCNNGC 3 cut(s) 282, 332, 511
BstDEI CTNAG 4 cut(s) 171, 337, 355, 530
BstF5I GGATG 1 cut(s) 459
BstFNI CGCG 1 cut(s) 515
BstHHI GCGC 2 cut(s) 291, 515
BstKTI GATC 5 cut(s) 81, 85, 186, 195, 462
BstMAI GTCTC 2 cut(s) 51, 137
BstMBI GATC 5 cut(s) 78, 82, 183, 192, 459
BstMCI CGRYCG 1 cut(s) 85
BstMWI GCNNNNNNNGC 7 cut(s) 286, 380, 389, 398, 413, 422, 442
BstNI CCWGG 3 cut(s) 30, 365, 610
BstSCI CCNGG 3 cut(s) 28, 363, 608
BstSFI CTRYAG 1 cut(s) 402
BstUI CGCG 1 cut(s) 515
BstV1I GCAGC 9 cut(s) 66, 69, 133, 247, 250, 264, 376, 388, 416
BstX2I RGATCY 2 cut(s) 183, 459
BstYI RGATCY 2 cut(s) 183, 459
Bsu15I ATCGAT 1 cut(s) 81
BsuRI GGCC 6 cut(s) 161, 206, 227, 419, 425, 528
BsuTUI ATCGAT 1 cut(s) 81
BtsCI GGATG 1 cut(s) 459
BtsI GCAGTG 1 cut(s) 284
BtsIMutI CAGTG 3 cut(s) 48, 162, 284
Cac8I GCNNGC 3 cut(s) 282, 332, 511
CciI TCATGA 1 cut(s) 189
CfoI GCGC 2 cut(s) 291, 515
Cfr13I GGNCC 1 cut(s) 226
ClaI ATCGAT 1 cut(s) 81
CsiI ACCWGGT 1 cut(s) 363
CviAII CATG 3 cut(s) 190, 220, 304
DdeI CTNAG 4 cut(s) 171, 337, 355, 530
DpnI GATC 5 cut(s) 80, 84, 185, 194, 461
DpnII GATC 5 cut(s) 78, 82, 183, 192, 459
EaeI YGGCCR 2 cut(s) 159, 423
Eam1104I CTCTTC 3 cut(s) 156, 329, 575
EarI CTCTTC 3 cut(s) 156, 329, 575
Eco130I CCWWGG 1 cut(s) 420
Eco31I GGTCTC 1 cut(s) 137
Eco57I CTGAAG 1 cut(s) 310
EcoO109I RGGNCCY 1 cut(s) 226
EcoRII CCWGG 3 cut(s) 28, 363, 608
EcoT14I CCWWGG 1 cut(s) 420
ErhI CCWWGG 1 cut(s) 420
FaeI CATG 3 cut(s) 193, 223, 307
FaiI YATR 9 cut(s) 191, 221, 299, 305, 350, 376, 378, 474, 582
FaqI GGGAC 1 cut(s) 23
FatI CATG 3 cut(s) 189, 219, 303
FauNDI CATATG 1 cut(s) 376
FbaI TGATCA 1 cut(s) 192
FokI GGATG 1 cut(s) 466
GlaI GCGC 2 cut(s) 290, 514
GsuI CTGGAG 1 cut(s) 592
HaeIII GGCC 6 cut(s) 161, 206, 227, 419, 425, 528
HhaI GCGC 2 cut(s) 291, 515
Hin1II CATG 3 cut(s) 193, 223, 307
Hin6I GCGC 2 cut(s) 289, 513
HinP1I GCGC 2 cut(s) 289, 513
HphI GGTGA 2 cut(s) 253, 379
Hpy166II GTNNAC 1 cut(s) 213
Hpy188I TCNGA 4 cut(s) 87, 137, 442, 575
Hpy188III TCNNGA 3 cut(s) 112, 190, 196
Hpy8I GTNNAC 1 cut(s) 213
HpyAV CCTTC 2 cut(s) 196, 217
HpyCH4III ACNGT 1 cut(s) 411
HpyCH4IV ACGT 1 cut(s) 468
HpyCH4V TGCA 6 cut(s) 280, 392, 404, 465, 486, 552
HpyF10VI GCNNNNNNNGC 7 cut(s) 286, 380, 389, 398, 413, 422, 442
HpyF3I CTNAG 4 cut(s) 171, 337, 355, 530
HpySE526I ACGT 1 cut(s) 468
Hsp92II CATG 3 cut(s) 193, 223, 307
HspAI GCGC 2 cut(s) 289, 513
Ksp22I TGATCA 1 cut(s) 192
Kzo9I GATC 5 cut(s) 78, 82, 183, 192, 459
LguI GCTCTTC 1 cut(s) 156
LmnI GCTCC 3 cut(s) 398, 442, 611
LpnPI CCDG 7 cut(s) 15, 42, 54, 158, 350, 377, 595
Lsp1109I GCAGC 9 cut(s) 66, 69, 133, 247, 250, 264, 376, 388, 416
MabI ACCWGGT 1 cut(s) 363
MaeII ACGT 1 cut(s) 468
MalI GATC 5 cut(s) 80, 84, 185, 194, 461
MboI GATC 5 cut(s) 78, 82, 183, 192, 459
MboII GAAGA 6 cut(s) 143, 178, 211, 316, 469, 562
MflI RGATCY 2 cut(s) 183, 459
MlsI TGGCCA 2 cut(s) 161, 425
MluCI AATT 1 cut(s) 504
MluNI TGGCCA 2 cut(s) 161, 425
MmeI TCCRAC 1 cut(s) 420
MnlI CCTC 6 cut(s) 87, 108, 305, 493, 539, 551
Mox20I TGGCCA 2 cut(s) 161, 425
MscI TGGCCA 2 cut(s) 161, 425
MslI CAYNNNNRTG 1 cut(s) 218
Msp20I TGGCCA 2 cut(s) 161, 425
MspA1I CMGCKG 2 cut(s) 146, 359
MspR9I CCNGG 3 cut(s) 30, 365, 610
MvaI CCWGG 3 cut(s) 30, 365, 610
MvnI CGCG 1 cut(s) 515
MwoI GCNNNNNNNGC 7 cut(s) 286, 380, 389, 398, 413, 422, 442
NdeI CATATG 1 cut(s) 376
NdeII GATC 5 cut(s) 78, 82, 183, 192, 459
NlaIII CATG 3 cut(s) 193, 223, 307
PagI TCATGA 1 cut(s) 189
PciSI GCTCTTC 1 cut(s) 156
PflMI CCANNNNNTGG 1 cut(s) 370
Ple19I CGATCG 1 cut(s) 85
Psp6I CCWGG 3 cut(s) 28, 363, 608
PspGI CCWGG 3 cut(s) 28, 363, 608
PspPI GGNCC 1 cut(s) 226
PsrI GAACNNNNNNTAC 2 cut(s) 464, 496
PstI CTGCAG 1 cut(s) 406
PsuI RGATCY 2 cut(s) 183, 459
PvuI CGATCG 1 cut(s) 85
PvuII CAGCTG 1 cut(s) 359
RseI CAYNNNNRTG 1 cut(s) 218
SapI GCTCTTC 1 cut(s) 156
Sau3AI GATC 5 cut(s) 78, 82, 183, 192, 459
Sau96I GGNCC 1 cut(s) 226
ScrFI CCNGG 3 cut(s) 30, 365, 610
SexAI ACCWGGT 1 cut(s) 363
SfcI CTRYAG 1 cut(s) 402
SmiMI CAYNNNNRTG 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 112
SmoI CTYRAG 1 cut(s) 112
Sse9I AATT 1 cut(s) 504
SsiI CCGC 3 cut(s) 144, 416, 515
StyD4I CCNGG 3 cut(s) 28, 363, 608
StyI CCWWGG 1 cut(s) 420
TaaI ACNGT 1 cut(s) 411
TaiI ACGT 1 cut(s) 471
TaqI TCGA 1 cut(s) 81
TasI AATT 1 cut(s) 504
TauI GCSGC 2 cut(s) 146, 419
TscAI CASTG 3 cut(s) 48, 169, 291
TspDTI ATGAA 3 cut(s) 178, 236, 470
TspGWI ACGGA 1 cut(s) 230
TspRI CASTG 3 cut(s) 48, 169, 291
Van91I CCANNNNNTGG 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.