Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0001290

Physical Location & Seq

Genomic Coordinates Forward (+)

425605 .. 430408

Gene Structure 4804 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0007200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1419 bp

ATGTCAGAGAAGTTCTCCGCCACGCTTCGAATCGGAGACCTTAACGACTACATAGCTCCCTCTCAGGCTTGCATAGTTTCTCTCAAATCCACCCCTACAAAGCCCTCTGACAAGTCTCAGGTTGCAACTTCCAGCAAGCAATTGAAAACAGAACCGGTTAAAATTTCACTCAAAGATTGCTTAGCATGCAGTGGGTGCATAACATCGGCCGAGACGGTTATGCTGGAGAAGCAAAGTTTGGATGAGTTTCTTTTGAATGTTGATAAAGGAAAAGCTGTCATTGTCTCCCTATCTCCCCAATCAAGAGCTTCACTTGCAGTTCATTTTGGGATTTCGCAACTTCAGGTCTTTAGGAAATTGACAACTCTTTTTAAGTCCCTGGGCGTAAAGGCTATATTTGACACAAGTTGCAGTAGAGATCTAAGTCTTATTGAATCTTGTAATGAATTCATGACAAGGTACAACCAGTCAACTGATGATGAAAGCCGTAAATCTTTGTTGCCCATGATTTCATCAGCATGTCCAGGCTGGATATGTTATGCTGAAAAAACACTTGGATCCTATGTACTGCCTTATATTTCTTCAGTGAAGAGTCCCCAGCAAACAATTGGAGTTATTGTTAAAAAGCATATATGCCAAAAAATGGGTCTTAGGCCAGATGATGTTTACCATGTAACAGTGATGCCTTGTTATGATAAGAAGCTTGAGGCGTCACGCGATGACTTCGTATTTGAAGTTGATGGAGCCAAGAGTAATGAGGATGGAAGTCTTAGCGTTTCAGAGGTAGATTCAGTGTTGACAACTGGGGAAGTTTTAGATCTGATACAGTTAAAAGGAGTTGATTTTGAGGGCTTAGAAGAGTCTCCACTAGATAGCATGTTGACAAACTTTAGTGACGGACATCTTTACGGAGTCCGCGGAAGCTCAGGGGGTTATGCAGAAACAATTTTCCGATATGCTGCTAAAGTAATCTTTGGAAGAGAAATTGAAGGCCCTGTGGACTTCAGAACTATTAAAAATTCAGACTTCCAGGAAGTGACTTTAGAGGTGGAAGGAAAGACTGTATTGAAATTTGCACTATGTTATGGATTTCGGAACCTGCAAAACGTTGTCAGGAAAATCAAGATTGGGAAATGTGATTATCATTTTCTAGAGATCATGGCATGCCCTTCTGGCTGCTTGAATGGTGGAGGACAGATAAAGCCAAAACCTGGGCAATCTGCAAAGCAGTTGATTCCATTATTACAAGCAGCTTATGCGGAAAATGTTTTGGTATCTGATCCTTTTGCAAATCCAATAGTGCAAAGCTTATACAATGAGTGGCTCGAGAAACCTGGTTCAGAGAAGGCCAGGCAACTCATGCACACAGAGTACCACCCTATCGTGAAAAGCATAACTTCTCAGTTACACAATTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006082 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009266 GO:0009314 GO:0009408 GO:0009414 GO:0009415 GO:0009416 GO:0009507 GO:0009526 GO:0009536 GO:0009540 GO:0009628 GO:0009639 GO:0009687 GO:0009688 GO:0009743 GO:0009756 GO:0009941 GO:0009987 GO:0010033 GO:0010035 GO:0010114 GO:0010182 GO:0016053 GO:0016106 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0016122 GO:0016123 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0019752 GO:0023052 GO:0030104 GO:0031967 GO:0031975 GO:0032501 GO:0032787 GO:0042221 GO:0042440 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043288 GO:0043289 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0046148 GO:0046165 GO:0046394 GO:0048871 GO:0048878 GO:0050789 GO:0050794 GO:0050878 GO:0050891 GO:0050896 GO:0051716 GO:0052662 GO:0055114 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071704 GO:0072330 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1901701 GO:1902644 GO:1902645

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD_binding_3

Protein Analysis

472

Amino Acids

52.1

Weight (kDa)

6.29

Isoelectric Point (pI)

44.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fe_hyd_lg_C	PF02906	91 - 397	4.6e-80	Iron only hydrogenase large subunit, C-terminal domain
Fe_hyd_SSU	PF02256	426 - 460	5.7e-11	Iron hydrogenase small subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001290)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g23740 FvH4_1g23750 FvH4_1g23770
malus_domestica	MD11G1231700.v1.1 MD11G1231900.v1.1
prunus_persica	Prupe.6G162100_v2.0.a1
pyrus_communis	pycom11g20520
rosa_chinensis	RchiOBHm_Chr2g0119591 RchiOBHm_Chr2g0119621 RchiOBHm_Chr2g0119641 RchiOBHm_Chr5g0013091
rosa_laevigata	RLG00000018140 RLG00000018142 RLG00000018474 RLG00000018477 RLG00000018479 RLG00000018481 RLG00000018482 RLG00000031985
rosa_multiflora	Rmu_sc0000825.1_g000006 Rmu_sc0000825.1_g000015 Rmu_sc0003158.1_g000005 Rmu_sc0003892.1_g000012 Rmu_sc0027906.1_g000001 Rmu_sc0040685.1_g000001
rosa_roxburghii	Rroxscaffold_1G00063490 Rroxscaffold_2G00124140 Rroxscaffold_2G00124230 Rroxscaffold_2G00124240 Rroxscaffold_2G00124250
rosa_rugosa	Rorug02G0222300 Rorug02G0222400 Rorug02G0222400 Rorug02G0222400 Rorug02G0222500.1 Rorug02G0223100 Rorug05G0007200
rosa_samantha	Rh2AG279800 Rh2AG280300 Rh2BG291500 Rh2BG291900 Rh2DG286500 Rh2DG286900 Rh2DG287300 Rh2DG305700 Rh2DG305900 Rh2DG306000 Rh5AG101300 Rh5BG097800 Rh5CG109900 Rh5DG096900
rosa_wichuraiana	Rw2G022360 Rw2G022400 Rw2G022410 Rw5G008850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1107
AccB7I	CCANNNNNTGG	2 cut(s)	643, 1211
AccII	CGCG	2 cut(s)	717, 918
AciI	CCGC	4 cut(s)	18, 916, 918, 1259
AclI	AACGTT	1 cut(s)	1107
AclWI	GGATC	3 cut(s)	552, 565, 1274
AcoI	YGGCCR	1 cut(s)	207
AcsI	RAATTY	4 cut(s)	162, 446, 1018, 1070
AcuI	CTGAAG	3 cut(s)	326, 567, 988
AcyI	GRCGYC	1 cut(s)	710
AfaI	GTAC	3 cut(s)	461, 567, 1373
AfiI	CCNNNNNNNGG	2 cut(s)	643, 1211
AgeI	ACCGGT	1 cut(s)	154
AgsI	TTSAA	7 cut(s)	145, 256, 434, 734, 989, 1069, 1183
AjnI	CCWGG	6 cut(s)	378, 523, 1029, 1210, 1333, 1349
AjuI	GAANNNNNNNTTGG	4 cut(s)	221, 253, 537, 569
AluBI	AGCT	7 cut(s)	56, 275, 308, 703, 924, 1253, 1308
AluI	AGCT	7 cut(s)	56, 275, 308, 703, 924, 1253, 1308
Alw26I	GTCTC	5 cut(s)	30, 120, 206, 289, 867
AlwI	GGATC	3 cut(s)	552, 565, 1274
Ama87I	CYCGRG	1 cut(s)	1325
AoxI	GGCC	4 cut(s)	207, 653, 991, 1347
ApeKI	GCWGC	3 cut(s)	959, 1176, 1250
ApoI	RAATTY	4 cut(s)	162, 446, 1018, 1070
ArsI	GACNNNNNNTTYG	4 cut(s)	631, 663, 713, 745
AsiGI	ACCGGT	1 cut(s)	154
AspS9I	GGNCC	1 cut(s)	992
AsuII	TTCGAA	1 cut(s)	28
AvaI	CYCGRG	1 cut(s)	1325
BamHI	GGATCC	1 cut(s)	557
BbvI	GCAGC	3 cut(s)	946, 1163, 1262
BccI	CCATC	2 cut(s)	734, 755
BceAI	ACGGC	1 cut(s)	471
BciT130I	CCWGG	6 cut(s)	380, 525, 1031, 1212, 1335, 1351
BcoDI	GTCTC	5 cut(s)	30, 120, 206, 289, 867
BfaI	CTAG	2 cut(s)	869, 1151
BfuAI	ACCTGC	1 cut(s)	1107
BglI	GCCNNNNNGGC	1 cut(s)	1173
BglII	AGATCT	2 cut(s)	418, 817
BisI	GCNGC	3 cut(s)	960, 1177, 1251
BlpI	GCTNAGC	1 cut(s)	181
BlsI	GCNGC	3 cut(s)	961, 1178, 1252
Bme1390I	CCNGG	6 cut(s)	380, 525, 1031, 1212, 1335, 1351
BmeT110I	CYCGRG	1 cut(s)	1325
BmgT120I	GGNCC	1 cut(s)	992
BmiI	GGNNCC	3 cut(s)	559, 745, 1097
BmrFI	CCNGG	6 cut(s)	380, 525, 1031, 1212, 1335, 1351
BmrI	ACTGGG	1 cut(s)	813
BmsI	GCATC	1 cut(s)	672
BmuI	ACTGGG	1 cut(s)	813
BplI	GAGNNNNNCTC	1 cut(s)	31
BpmI	CTGGAG	1 cut(s)	245
Bpu10I	CCTNAGC	1 cut(s)	925
Bpu1102I	GCTNAGC	1 cut(s)	181
Bpu14I	TTCGAA	1 cut(s)	28
BpuEI	CTTGAG	1 cut(s)	725
BsaHI	GRCGYC	1 cut(s)	710
BsaI	GGTCTC	1 cut(s)	30
BsaJI	CCNNGG	4 cut(s)	378, 379, 916, 1211
BsaWI	WCCGGW	1 cut(s)	154
BsaXI	ACNNNNNCTCC	2 cut(s)	603, 633
Bsc4I	CCNNNNNNNGG	2 cut(s)	643, 1211
Bse118I	RCCGGY	1 cut(s)	154
Bse1I	ACTGG	2 cut(s)	466, 808
BseBI	CCWGG	6 cut(s)	380, 525, 1031, 1212, 1335, 1351
BseDI	CCNNGG	4 cut(s)	378, 379, 916, 1211
BseGI	GGATG	2 cut(s)	247, 766
BseLI	CCNNNNNNNGG	2 cut(s)	643, 1211
BseMII	CTCAG	4 cut(s)	77, 131, 939, 1415
BseNI	ACTGG	2 cut(s)	466, 808
BseX3I	CGGCCG	1 cut(s)	207
BseXI	GCAGC	3 cut(s)	946, 1163, 1262
BseYI	CCCAGC	1 cut(s)	597
Bsh1236I	CGCG	2 cut(s)	717, 918
Bsh1285I	CGRYCG	1 cut(s)	210
BshFI	GGCC	4 cut(s)	209, 655, 993, 1349
BshTI	ACCGGT	1 cut(s)	154
BsiEI	CGRYCG	1 cut(s)	210
BsiHKCI	CYCGRG	1 cut(s)	1325
BsiSI	CCGG	1 cut(s)	155
BslFI	GGGAC	2 cut(s)	361, 579
BslI	CCNNNNNNNGG	2 cut(s)	643, 1211
BsmAI	GTCTC	5 cut(s)	30, 120, 206, 289, 867
BsmBI	CGTCTC	1 cut(s)	206
BsmFI	GGGAC	2 cut(s)	361, 579
BsnI	GGCC	4 cut(s)	209, 655, 993, 1349
Bso31I	GGTCTC	1 cut(s)	30
BsoBI	CYCGRG	1 cut(s)	1325
Bsp119I	TTCGAA	1 cut(s)	28
Bsp143I	GATC	5 cut(s)	418, 557, 817, 1155, 1279
Bsp1720I	GCTNAGC	1 cut(s)	181
BspACI	CCGC	4 cut(s)	18, 916, 918, 1259
BspANI	GGCC	4 cut(s)	209, 655, 993, 1349
BspCNI	CTCAG	4 cut(s)	76, 130, 938, 1414
BspFNI	CGCG	2 cut(s)	717, 918
BspHI	TCATGA	1 cut(s)	450
BspLI	GGNNCC	3 cut(s)	559, 745, 1097
BspMI	ACCTGC	1 cut(s)	1107
BspPI	GGATC	3 cut(s)	552, 565, 1274
BspT104I	TTCGAA	1 cut(s)	28
BspTNI	GGTCTC	1 cut(s)	30
BsrFI	RCCGGY	1 cut(s)	154
BsrI	ACTGG	2 cut(s)	466, 808
BssAI	RCCGGY	1 cut(s)	154
BssECI	CCNNGG	4 cut(s)	378, 379, 916, 1211
BssMI	GATC	5 cut(s)	418, 557, 817, 1155, 1279
BssNI	GRCGYC	1 cut(s)	710
Bst2UI	CCWGG	6 cut(s)	380, 525, 1031, 1212, 1335, 1351
Bst4CI	ACNGT	4 cut(s)	217, 679, 828, 1063
Bst6I	CTCTTC	3 cut(s)	584, 852, 973
BstACI	GRCGYC	1 cut(s)	710
BstAPI	GCANNNNNTGC	3 cut(s)	195, 1256, 1360
BstBI	TTCGAA	1 cut(s)	28
BstC8I	GCNNGC	4 cut(s)	70, 137, 187, 1165
BstDEI	CTNAG	9 cut(s)	63, 117, 181, 422, 650, 770, 853, 925, 1401
BstDSI	CCRYGG	1 cut(s)	916
BstF5I	GGATG	2 cut(s)	247, 766
BstFNI	CGCG	2 cut(s)	717, 918
BstKTI	GATC	5 cut(s)	421, 560, 820, 1158, 1282
BstMAI	GTCTC	5 cut(s)	30, 120, 206, 289, 867
BstMBI	GATC	5 cut(s)	418, 557, 817, 1155, 1279
BstMCI	CGRYCG	1 cut(s)	210
BstMWI	GCNNNNNNNGC	7 cut(s)	186, 195, 229, 314, 1173, 1256, 1360
BstNI	CCWGG	6 cut(s)	380, 525, 1031, 1212, 1335, 1351
BstNSI	RCATGY	4 cut(s)	189, 522, 880, 1167
BstSCI	CCNGG	6 cut(s)	378, 523, 1029, 1210, 1333, 1349
BstUI	CGCG	2 cut(s)	717, 918
BstV1I	GCAGC	3 cut(s)	946, 1163, 1262
BstX2I	RGATCY	3 cut(s)	418, 557, 817
BstYI	RGATCY	3 cut(s)	418, 557, 817
BstZI	CGGCCG	1 cut(s)	207
BsuRI	GGCC	4 cut(s)	209, 655, 993, 1349
BtgI	CCRYGG	1 cut(s)	916
BtgZI	GCGATG	1 cut(s)	732
BtsCI	GGATG	2 cut(s)	247, 766
BtsI	GCAGTG	1 cut(s)	196
BtsIMutI	CAGTG	4 cut(s)	196, 591, 684, 798
BveI	ACCTGC	1 cut(s)	1107
Cac8I	GCNNGC	4 cut(s)	70, 137, 187, 1165
CciI	TCATGA	1 cut(s)	450
Cfr10I	RCCGGY	1 cut(s)	154
Cfr13I	GGNCC	1 cut(s)	992
Cfr42I	CCGCGG	1 cut(s)	919
CseI	GACGC	1 cut(s)	699
CsiI	ACCWGGT	1 cut(s)	1333
Csp6I	GTAC	3 cut(s)	460, 566, 1372
CspAI	ACCGGT	1 cut(s)	154
CviAII	CATG	9 cut(s)	186, 451, 505, 519, 671, 877, 1159, 1164, 1360
CviQI	GTAC	3 cut(s)	460, 566, 1372
DdeI	CTNAG	9 cut(s)	63, 117, 181, 422, 650, 770, 853, 925, 1401
DpnI	GATC	5 cut(s)	420, 559, 819, 1157, 1281
DpnII	GATC	5 cut(s)	418, 557, 817, 1155, 1279
EaeI	YGGCCR	1 cut(s)	207
EagI	CGGCCG	1 cut(s)	207
Eam1104I	CTCTTC	3 cut(s)	584, 852, 973
EarI	CTCTTC	3 cut(s)	584, 852, 973
EciI	GGCGGA	1 cut(s)	7
EclXI	CGGCCG	1 cut(s)	207
Eco31I	GGTCTC	1 cut(s)	30
Eco52I	CGGCCG	1 cut(s)	207
Eco57I	CTGAAG	3 cut(s)	326, 567, 988
Eco88I	CYCGRG	1 cut(s)	1325
EcoO109I	RGGNCCY	1 cut(s)	992
EcoRI	GAATTC	1 cut(s)	446
EcoRII	CCWGG	6 cut(s)	378, 523, 1029, 1210, 1333, 1349
Esp3I	CGTCTC	1 cut(s)	206
FaeI	CATG	9 cut(s)	189, 454, 508, 522, 674, 880, 1162, 1167, 1363
FalI	AAGNNNNNCTT	2 cut(s)	1381, 1413
FaqI	GGGAC	2 cut(s)	361, 579
FatI	CATG	9 cut(s)	185, 450, 504, 518, 670, 876, 1158, 1163, 1359
Fnu4HI	GCNGC	3 cut(s)	960, 1177, 1251
FokI	GGATG	2 cut(s)	254, 773
Fsp4HI	GCNGC	3 cut(s)	960, 1177, 1251
FspBI	CTAG	2 cut(s)	869, 1151
GluI	GCNGC	3 cut(s)	960, 1177, 1251
GsaI	CCCAGC	1 cut(s)	601
GsuI	CTGGAG	1 cut(s)	245
HaeIII	GGCC	4 cut(s)	209, 655, 993, 1349
HapII	CCGG	1 cut(s)	155
HgaI	GACGC	1 cut(s)	699
Hin1I	GRCGYC	1 cut(s)	710
Hin1II	CATG	9 cut(s)	189, 454, 508, 522, 674, 880, 1162, 1167, 1363
HincII	GTYRAC	3 cut(s)	471, 798, 882
HindII	GTYRAC	3 cut(s)	471, 798, 882
HindIII	AAGCTT	2 cut(s)	701, 1306
HinfI	GANTC	7 cut(s)	30, 434, 592, 788, 860, 912, 1234
HpaII	CCGG	1 cut(s)	155
Hpy166II	GTNNAC	5 cut(s)	471, 667, 798, 882, 1000
Hpy188III	TCNNGA	7 cut(s)	303, 451, 1114, 1123, 1151, 1327, 1384
Hpy8I	GTNNAC	5 cut(s)	471, 667, 798, 882, 1000
HpyAV	CCTTC	4 cut(s)	983, 1046, 1179, 1339
HpyCH4III	ACNGT	4 cut(s)	217, 679, 828, 1063
HpyCH4IV	ACGT	1 cut(s)	1107
HpyF10VI	GCNNNNNNNGC	7 cut(s)	186, 195, 229, 314, 1173, 1256, 1360
HpyF3I	CTNAG	9 cut(s)	63, 117, 181, 422, 650, 770, 853, 925, 1401
HpySE526I	ACGT	1 cut(s)	1107
Hsp92I	GRCGYC	1 cut(s)	710
Hsp92II	CATG	9 cut(s)	189, 454, 508, 522, 674, 880, 1162, 1167, 1363
KspI	CCGCGG	1 cut(s)	919
Kzo9I	GATC	5 cut(s)	418, 557, 817, 1155, 1279
LmnI	GCTCC	2 cut(s)	61, 743
Lsp1109I	GCAGC	3 cut(s)	946, 1163, 1262
LweI	GCATC	1 cut(s)	672
MabI	ACCWGGT	1 cut(s)	1333
MaeI	CTAG	2 cut(s)	869, 1151
MaeII	ACGT	1 cut(s)	1107
MaeIII	GTNAC	5 cut(s)	673, 711, 893, 1036, 1404
MalI	GATC	5 cut(s)	420, 559, 819, 1157, 1281
MboI	GATC	5 cut(s)	418, 557, 817, 1155, 1279
MboII	GAAGA	4 cut(s)	573, 601, 869, 990
MfeI	CAATTG	3 cut(s)	140, 606, 1411
MflI	RGATCY	3 cut(s)	418, 557, 817
MlyI	GAGTC	3 cut(s)	601, 869, 921
MnlI	CCTC	8 cut(s)	70, 115, 700, 751, 775, 841, 1039, 1184
MseI	TTAA	6 cut(s)	42, 159, 372, 621, 830, 1014
MslI	CAYNNNNRTG	1 cut(s)	517
MspA1I	CMGCKG	1 cut(s)	918
MspI	CCGG	1 cut(s)	155
MspR9I	CCNGG	6 cut(s)	380, 525, 1031, 1212, 1335, 1351
MunI	CAATTG	3 cut(s)	140, 606, 1411
MvaI	CCWGG	6 cut(s)	380, 525, 1031, 1212, 1335, 1351
MvnI	CGCG	2 cut(s)	717, 918
MwoI	GCNNNNNNNGC	7 cut(s)	186, 195, 229, 314, 1173, 1256, 1360
NdeII	GATC	5 cut(s)	418, 557, 817, 1155, 1279
NlaIII	CATG	9 cut(s)	189, 454, 508, 522, 674, 880, 1162, 1167, 1363
NlaIV	GGNNCC	3 cut(s)	559, 745, 1097
NmeAIII	GCCGAG	1 cut(s)	235
NmuCI	GTSAC	3 cut(s)	711, 893, 1036
NspI	RCATGY	4 cut(s)	189, 522, 880, 1167
NspV	TTCGAA	1 cut(s)	28
PaeI	GCATGC	2 cut(s)	189, 1167
PaeR7I	CTCGAG	1 cut(s)	1325
PagI	TCATGA	1 cut(s)	450
PasI	CCCWGGG	1 cut(s)	379
PfeI	GAWTC	4 cut(s)	30, 434, 788, 1234
PflMI	CCANNNNNTGG	2 cut(s)	643, 1211
PfoI	TCCNGGA	1 cut(s)	1029
PinAI	ACCGGT	1 cut(s)	154
PkrI	GCNGC	3 cut(s)	961, 1178, 1252
PleI	GAGTC	3 cut(s)	600, 868, 920
PpsI	GAGTC	3 cut(s)	600, 868, 920
Psp1406I	AACGTT	1 cut(s)	1107
Psp6I	CCWGG	6 cut(s)	378, 523, 1029, 1210, 1333, 1349
PspFI	CCCAGC	1 cut(s)	597
PspGI	CCWGG	6 cut(s)	378, 523, 1029, 1210, 1333, 1349
PspN4I	GGNNCC	3 cut(s)	559, 745, 1097
PspPI	GGNCC	1 cut(s)	992
PsuI	RGATCY	3 cut(s)	418, 557, 817
RsaI	GTAC	3 cut(s)	461, 567, 1373
RsaNI	GTAC	3 cut(s)	460, 566, 1372
RseI	CAYNNNNRTG	1 cut(s)	517
SacII	CCGCGG	1 cut(s)	919
SaqAI	TTAA	6 cut(s)	42, 159, 372, 621, 830, 1014
SatI	GCNGC	3 cut(s)	960, 1177, 1251
Sau3AI	GATC	5 cut(s)	418, 557, 817, 1155, 1279
Sau96I	GGNCC	1 cut(s)	992
SchI	GAGTC	3 cut(s)	601, 869, 921
ScrFI	CCNGG	6 cut(s)	380, 525, 1031, 1212, 1335, 1351
SexAI	ACCWGGT	1 cut(s)	1333
SfaNI	GCATC	1 cut(s)	672
Sfr274I	CTCGAG	1 cut(s)	1325
Sfr303I	CCGCGG	1 cut(s)	919
SfuI	TTCGAA	1 cut(s)	28
SgrBI	CCGCGG	1 cut(s)	919
SlaI	CTCGAG	1 cut(s)	1325
SmiMI	CAYNNNNRTG	1 cut(s)	517
SmlI	CTYRAG	2 cut(s)	704, 1325
SmoI	CTYRAG	2 cut(s)	704, 1325
SphI	GCATGC	2 cut(s)	189, 1167
SsiI	CCGC	4 cut(s)	18, 916, 918, 1259
SspMI	CTAG	2 cut(s)	869, 1151
StyD4I	CCNGG	6 cut(s)	378, 523, 1029, 1210, 1333, 1349
TaaI	ACNGT	4 cut(s)	217, 679, 828, 1063
TaiI	ACGT	1 cut(s)	1110
TaqI	TCGA	2 cut(s)	28, 1326
TatI	WGTACW	1 cut(s)	565
TfiI	GAWTC	4 cut(s)	30, 434, 788, 1234
Tru1I	TTAA	6 cut(s)	42, 159, 372, 621, 830, 1014
Tru9I	TTAA	6 cut(s)	42, 159, 372, 621, 830, 1014
TscAI	CASTG	4 cut(s)	196, 591, 684, 798
TseFI	GTSAC	3 cut(s)	711, 893, 1036
TseI	GCWGC	3 cut(s)	959, 1176, 1250
Tsp45I	GTSAC	3 cut(s)	711, 893, 1036
TspDTI	ATGAA	5 cut(s)	311, 439, 459, 495, 501
TspGWI	ACGGA	2 cut(s)	912, 924
TspRI	CASTG	4 cut(s)	196, 591, 684, 798
Van91I	CCANNNNNTGG	2 cut(s)	643, 1211
XapI	RAATTY	4 cut(s)	162, 446, 1018, 1070
XbaI	TCTAGA	1 cut(s)	1150
XceI	RCATGY	4 cut(s)	189, 522, 880, 1167
XcmI	CCANNNNNNNNNTGG	1 cut(s)	605
XhoI	CTCGAG	1 cut(s)	1325
XspI	CTAG	2 cut(s)	869, 1151

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.