Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0001290

Physical Location & Seq

Genomic Coordinates Reverse (-)

25827963 .. 25832964

Gene Structure 5002 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000018140

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1440 bp

ATGGGCACAAGTGTGGAGGATGTGGTGATAGTCGGAGGAGGGATAGCCGGGCTGGCGACAGCGGTGGCGCTGAAGAGAGCTGGAATAGAAGCGTTGGTGTTGGAGAGATCGGAAGGGCTGCGAACCACTGGTGCAGCCTTGTCTCTATTCTCAAACGCCTGGTGTGCTCTTGATGCCTTGGGGGTTTCTCACAAGCTTGCTTCTTATACCCCCTCTCTCAAGAATTATGTAACTGATCTTGATACTGGAGAAGTTCAAGAAGTCTCTTTCACCGAAGCCAATGGTGATGCAGTTGGACCGAGAACAGTACATCGAAAAGCCTTGCTCGAGGCTTTGGCAGATGAATTATCGGTTCATTCAATCCGTTTCTCTTCCAAGATCTCTGCTATCGAAACCCAACAACATGAAGGTTCATCCATTGCCATTGTTCACATGGAAAATGGAACTGTCATTAAAGCAAAGGTTCTCATAGGGTGTGACGGGGTTCACTCAGTGGTGTCACGCTGGTTAGGACTCCGTGAACCAGTCTTTTCAGGTCGATCAGCTGTTCGTGGCTTGGCTGTTTATCCTCAAGGCCATGGACTCGACCAGAATGTGCGACAGTATGTAGGATCAGGCAGAAGGGCTGGCTTCGTTCCCCTCACCGACAAAGAGATCTATTGGTTCTTCTCTGGCATATCTCCAGCTAAAGGGACCTCCCTGGCAGAAGATCCTGAAGATATACAAAAAGAAGTACTTGAGAATTATGCCAAGGACCTCCCACCTATATACTTAGATGTTGTGCAGCACGCAGATCTTTCGACATTAACATGGGCTCCGTTGATGTTTAGGTACCCCTGGAACGTAGTATTTGGAAACTTAAGCAAGCAAAACATCACGGTAGCTGGCGATGCCCTGCACCCCATGACCCCTGATCTAGGACAAGGGGGTTGCTCAGCATTAGAAGATGCAGTGATCTTAGGCAGGCACATCGGGACATCCTTTGTACAGAATGGACAAGTACTTGTGCTGAAAAAGATGAGTGAAGTACTGGGCAAATACGTAGAAGAAAGAAGGTGGCGCGTCACTTTGTTGATCATGTGGTCCTATATATCTGGATGGGTGCAGCAGGGAGGATCTGGATGGGGTATGAGATTCTTGAGAGACGCCATATTTTACAACGATACAGTAAATGAAACGGTAAGGTACCTAATTGCTCGAACCCGACCCTATTTCACTTGGCCGAATGAGCGAGTCGGTGGAGGTGTCGAGAATGTTGGCCTGCTGGAACTCCAGCTCGGTGAGCTGCTGCTCGCGGTCCATCTCGATGATGAGAGGGTCGACGAGGATGAGGAAGGTGGTGCCGAAGATCCAGGCAGCCTTACCGGTGCTCTTCAGCAGCTTCTTGGCAACTTAGCCGGCGTCGGAGGTGACTGGGAGATCCTAGCGATGATGTCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006082 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009266 GO:0009314 GO:0009408 GO:0009414 GO:0009415 GO:0009416 GO:0009507 GO:0009526 GO:0009536 GO:0009540 GO:0009628 GO:0009639 GO:0009687 GO:0009688 GO:0009743 GO:0009756 GO:0009941 GO:0009987 GO:0010033 GO:0010035 GO:0010114 GO:0010182 GO:0016053 GO:0016106 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0016122 GO:0016123 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0019752 GO:0023052 GO:0030104 GO:0031967 GO:0031975 GO:0032501 GO:0032787 GO:0042221 GO:0042440 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043288 GO:0043289 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0046148 GO:0046165 GO:0046394 GO:0048871 GO:0048878 GO:0050789 GO:0050794 GO:0050878 GO:0050891 GO:0050896 GO:0051716 GO:0052662 GO:0055114 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071704 GO:0072330 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1901701 GO:1902644 GO:1902645

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD_binding_3

Protein Analysis

480

Amino Acids

51.98

Weight (kDa)

5.09

Isoelectric Point (pI)

35.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_3	PF01494	7 - 359	4.6e-25	FAD binding domain
DAO	PF01266	7 - 57	2.3e-08	FAD dependent oxidoreductase
Lycopene_cycl	PF05834	7 - 164	2.5e-06	Lycopene cyclase protein
FAD_oxidored	PF12831	7 - 159	4.9e-06	FAD dependent oxidoreductase
NAD_binding_8	PF13450	10 - 40	1e-05	NAD(P)-binding Rossmann-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001290)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g23740 FvH4_1g23750 FvH4_1g23770
malus_domestica	MD11G1231700.v1.1 MD11G1231900.v1.1
prunus_persica	Prupe.6G162100_v2.0.a1
pyrus_communis	pycom11g20520
rosa_chinensis	RchiOBHm_Chr2g0119591 RchiOBHm_Chr2g0119621 RchiOBHm_Chr2g0119641 RchiOBHm_Chr5g0013091
rosa_laevigata	RLG00000018140 RLG00000018142 RLG00000018474 RLG00000018477 RLG00000018479 RLG00000018481 RLG00000018482 RLG00000031985
rosa_multiflora	Rmu_sc0000825.1_g000006 Rmu_sc0000825.1_g000015 Rmu_sc0003158.1_g000005 Rmu_sc0003892.1_g000012 Rmu_sc0027906.1_g000001 Rmu_sc0040685.1_g000001
rosa_roxburghii	Rroxscaffold_1G00063490 Rroxscaffold_2G00124140 Rroxscaffold_2G00124230 Rroxscaffold_2G00124240 Rroxscaffold_2G00124250
rosa_rugosa	Rorug02G0222300 Rorug02G0222400 Rorug02G0222400 Rorug02G0222400 Rorug02G0222500.1 Rorug02G0223100 Rorug05G0007200
rosa_samantha	Rh2AG279800 Rh2AG280300 Rh2BG291500 Rh2BG291900 Rh2DG286500 Rh2DG286900 Rh2DG287300 Rh2DG305700 Rh2DG305900 Rh2DG306000 Rh5AG101300 Rh5BG097800 Rh5CG109900 Rh5DG096900
rosa_wichuraiana	Rw2G022360 Rw2G022400 Rw2G022410 Rw5G008850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	2 cut(s)	831, 1185
AccB1I	GGYRCC	3 cut(s)	831, 1185, 1340
AccI	GTMKAC	1 cut(s)	1320
AccII	CGCG	2 cut(s)	1062, 1295
AciI	CCGC	2 cut(s)	62, 1295
AclWI	GGATC	5 cut(s)	619, 704, 1123, 1343, 1414
AcoI	YGGCCR	1 cut(s)	1220
AcuI	CTGAAG	3 cut(s)	92, 735, 1358
AcyI	GRCGYC	2 cut(s)	1146, 1401
AdeI	CACNNNGTG	1 cut(s)	493
AfaI	GTAC	7 cut(s)	309, 735, 833, 987, 1002, 1029, 1187
AfiI	CCNNNNNNNGG	2 cut(s)	689, 917
AflII	CTTAAG	1 cut(s)	859
AgeI	ACCGGT	1 cut(s)	1364
AgsI	TTSAA	2 cut(s)	257, 360
AjnI	CCWGG	4 cut(s)	158, 699, 836, 1351
AleI	CACNNNNGTG	1 cut(s)	11
AluBI	AGCT	8 cut(s)	80, 196, 545, 686, 884, 1276, 1285, 1381
AluI	AGCT	8 cut(s)	80, 196, 545, 686, 884, 1276, 1285, 1381
Alw21I	GWGCWC	2 cut(s)	169, 1372
Alw26I	GTCTC	3 cut(s)	147, 268, 1137
AlwI	GGATC	5 cut(s)	619, 704, 1123, 1343, 1414
Ama87I	CYCGRG	1 cut(s)	326
AoxI	GGCC	3 cut(s)	574, 1220, 1258
ApeKI	GCWGC	8 cut(s)	118, 134, 784, 1105, 1285, 1288, 1356, 1378
AsiGI	ACCGGT	1 cut(s)	1364
Asp718I	GGTACC	2 cut(s)	831, 1185
AspLEI	GCGC	2 cut(s)	70, 1062
AspS9I	GGNCC	5 cut(s)	296, 693, 754, 1083, 1297
AsuC2I	CCSGG	1 cut(s)	49
AsuHPI	GGTGA	6 cut(s)	37, 262, 296, 634, 1292, 1421
AvaI	CYCGRG	1 cut(s)	326
AvaII	GGWCC	5 cut(s)	296, 693, 754, 1083, 1297
BaeGI	GKGCMC	1 cut(s)	8
BanI	GGYRCC	3 cut(s)	831, 1185, 1340
BanII	GRGCYC	1 cut(s)	817
Bbv12I	GWGCWC	2 cut(s)	169, 1372
BbvI	GCAGC	8 cut(s)	105, 146, 796, 1117, 1272, 1275, 1368, 1390
BccI	CCATC	3 cut(s)	1092, 1116, 1308
BcgI	CGANNNNNNTGC	4 cut(s)	780, 814, 952, 986
BciT130I	CCWGG	4 cut(s)	160, 701, 838, 1353
BclI	TGATCA	1 cut(s)	1074
BcnI	CCSGG	1 cut(s)	49
BcoDI	GTCTC	3 cut(s)	147, 268, 1137
BfaI	CTAG	2 cut(s)	917, 1424
BfoI	RGCGCY	1 cut(s)	71
BfrI	CTTAAG	1 cut(s)	859
BglI	GCCNNNNNGGC	1 cut(s)	53
BglII	AGATCT	3 cut(s)	378, 654, 793
BisI	GCNGC	8 cut(s)	119, 135, 785, 1106, 1286, 1289, 1357, 1379
BlpI	GCTNAGC	1 cut(s)	934
BlsI	GCNGC	8 cut(s)	120, 136, 786, 1107, 1287, 1290, 1358, 1380
BmcAI	AGTACT	3 cut(s)	735, 1002, 1029
Bme1390I	CCNGG	5 cut(s)	49, 160, 701, 838, 1353
Bme18I	GGWCC	5 cut(s)	296, 693, 754, 1083, 1297
BmeT110I	CYCGRG	1 cut(s)	326
BmgT120I	GGNCC	5 cut(s)	296, 693, 754, 1083, 1297
BmiI	GGNNCC	5 cut(s)	694, 816, 833, 1187, 1342
BmrFI	CCNGG	5 cut(s)	49, 160, 701, 838, 1353
BmrI	ACTGGG	2 cut(s)	1040, 1423
BmsI	GCATC	4 cut(s)	163, 277, 880, 937
BmuI	ACTGGG	2 cut(s)	1040, 1423
BpmI	CTGGAG	3 cut(s)	267, 666, 1256
Bpu1102I	GCTNAGC	1 cut(s)	934
BpuEI	CTTGAG	4 cut(s)	203, 555, 758, 1159
BpuMI	CCSGG	1 cut(s)	49
BsaAI	YACGTR	1 cut(s)	1042
BsaHI	GRCGYC	2 cut(s)	1146, 1401
BsaJI	CCNNGG	5 cut(s)	177, 577, 699, 750, 836
BsaWI	WCCGGW	1 cut(s)	1364
BsaXI	ACNNNNNCTCC	2 cut(s)	799, 829
Bsc4I	CCNNNNNNNGG	2 cut(s)	689, 917
Bse118I	RCCGGY	2 cut(s)	1364, 1397
Bse1I	ACTGG	5 cut(s)	133, 250, 524, 1035, 1418
Bse3DI	GCAATG	1 cut(s)	417
BseBI	CCWGG	4 cut(s)	160, 701, 838, 1353
BseDI	CCNNGG	5 cut(s)	177, 577, 699, 750, 836
BseGI	GGATG	6 cut(s)	25, 413, 977, 1103, 1127, 1333
BseLI	CCNNNNNNNGG	2 cut(s)	689, 917
BseMI	GCAATG	1 cut(s)	417
BseMII	CTCAG	2 cut(s)	504, 948
BseNI	ACTGG	5 cut(s)	133, 250, 524, 1035, 1418
BseRI	GAGGAG	1 cut(s)	51
BseSI	GKGCMC	1 cut(s)	8
BseXI	GCAGC	8 cut(s)	105, 146, 796, 1117, 1272, 1275, 1368, 1390
BsgI	GTGCAG	4 cut(s)	153, 803, 881, 1124
Bsh1236I	CGCG	2 cut(s)	1062, 1295
BshFI	GGCC	3 cut(s)	576, 1222, 1260
BshNI	GGYRCC	3 cut(s)	831, 1185, 1340
BshTI	ACCGGT	1 cut(s)	1364
BsiHKAI	GWGCWC	2 cut(s)	169, 1372
BsiHKCI	CYCGRG	1 cut(s)	326
BsiSI	CCGG	3 cut(s)	48, 1365, 1398
BslFI	GGGAC	2 cut(s)	706, 988
BslI	CCNNNNNNNGG	2 cut(s)	689, 917
BsmAI	GTCTC	3 cut(s)	147, 268, 1137
BsmBI	CGTCTC	1 cut(s)	1137
BsmFI	GGGAC	2 cut(s)	706, 988
BsnI	GGCC	3 cut(s)	576, 1222, 1260
BsoBI	CYCGRG	1 cut(s)	326
Bsp1286I	GDGCHC	4 cut(s)	8, 169, 817, 1372
Bsp1407I	TGTACA	1 cut(s)	985
Bsp1720I	GCTNAGC	1 cut(s)	934
Bsp19I	CCATGG	1 cut(s)	577
BspACI	CCGC	2 cut(s)	62, 1295
BspANI	GGCC	3 cut(s)	576, 1222, 1260
BspCNI	CTCAG	2 cut(s)	503, 947
BspFNI	CGCG	2 cut(s)	1062, 1295
BspLI	GGNNCC	5 cut(s)	694, 816, 833, 1187, 1342
BspPI	GGATC	5 cut(s)	619, 704, 1123, 1343, 1414
BspQI	GCTCTTC	1 cut(s)	1377
BspT107I	GGYRCC	3 cut(s)	831, 1185, 1340
BspTI	CTTAAG	1 cut(s)	859
BsrDI	GCAATG	1 cut(s)	417
BsrFI	RCCGGY	2 cut(s)	1364, 1397
BsrGI	TGTACA	1 cut(s)	985
BsrI	ACTGG	5 cut(s)	133, 250, 524, 1035, 1418
BssAI	RCCGGY	2 cut(s)	1364, 1397
BssECI	CCNNGG	5 cut(s)	177, 577, 699, 750, 836
BssNI	GRCGYC	2 cut(s)	1146, 1401
BssT1I	CCWWGG	3 cut(s)	177, 577, 750
Bst2UI	CCWGG	4 cut(s)	160, 701, 838, 1353
Bst4CI	ACNGT	6 cut(s)	307, 448, 603, 880, 1168, 1180
Bst6I	CTCTTC	3 cut(s)	68, 376, 1377
BstACI	GRCGYC	2 cut(s)	1146, 1401
BstAFI	CTTAAG	1 cut(s)	859
BstAUI	TGTACA	1 cut(s)	985
BstBAI	YACGTR	1 cut(s)	1042
BstDEI	CTNAG	5 cut(s)	490, 772, 934, 958, 1393
BstDSI	CCRYGG	1 cut(s)	577
BstENI	CCTNNNNNAGG	1 cut(s)	915
BstF5I	GGATG	6 cut(s)	25, 413, 977, 1103, 1127, 1333
BstFNI	CGCG	2 cut(s)	1062, 1295
BstH2I	RGCGCY	1 cut(s)	71
BstHHI	GCGC	2 cut(s)	70, 1062
BstMAI	GTCTC	3 cut(s)	147, 268, 1137
BstMWI	GCNNNNNNNGC	6 cut(s)	53, 164, 173, 890, 1228, 1282
BstNI	CCWGG	4 cut(s)	160, 701, 838, 1353
BstSCI	CCNGG	5 cut(s)	47, 158, 699, 836, 1351
BstSLI	GKGCMC	1 cut(s)	8
BstSNI	TACGTA	1 cut(s)	1042
BstUI	CGCG	2 cut(s)	1062, 1295
BstV1I	GCAGC	8 cut(s)	105, 146, 796, 1117, 1272, 1275, 1368, 1390
BstX2I	RGATCY	7 cut(s)	378, 654, 709, 793, 1115, 1348, 1419
BstYI	RGATCY	7 cut(s)	378, 654, 709, 793, 1115, 1348, 1419
BsuRI	GGCC	3 cut(s)	576, 1222, 1260
BtgI	CCRYGG	1 cut(s)	577
BtgZI	GCGATG	1 cut(s)	903
BtsCI	GGATG	6 cut(s)	25, 413, 977, 1103, 1127, 1333
BtsI	GCAGTG	1 cut(s)	957
BtsIMutI	CAGTG	3 cut(s)	126, 498, 957
CfoI	GCGC	2 cut(s)	70, 1062
Cfr10I	RCCGGY	2 cut(s)	1364, 1397
Cfr13I	GGNCC	5 cut(s)	296, 693, 754, 1083, 1297
CseI	GACGC	3 cut(s)	1051, 1154, 1390
Csp6I	GTAC	7 cut(s)	308, 734, 832, 986, 1001, 1028, 1186
CspAI	ACCGGT	1 cut(s)	1364
CviAII	CATG	6 cut(s)	404, 433, 578, 810, 904, 1078
CviQI	GTAC	7 cut(s)	308, 734, 832, 986, 1001, 1028, 1186
DdeI	CTNAG	5 cut(s)	490, 772, 934, 958, 1393
DraIII	CACNNNGTG	1 cut(s)	493
EaeI	YGGCCR	1 cut(s)	1220
Eam1104I	CTCTTC	3 cut(s)	68, 376, 1377
EarI	CTCTTC	3 cut(s)	68, 376, 1377
Eco105I	TACGTA	1 cut(s)	1042
Eco130I	CCWWGG	3 cut(s)	177, 577, 750
Eco24I	GRGCYC	1 cut(s)	817
Eco47I	GGWCC	5 cut(s)	296, 693, 754, 1083, 1297
Eco57I	CTGAAG	3 cut(s)	92, 735, 1358
Eco88I	CYCGRG	1 cut(s)	326
EcoNI	CCTNNNNNAGG	1 cut(s)	915
EcoO109I	RGGNCCY	2 cut(s)	693, 754
EcoRII	CCWGG	4 cut(s)	158, 699, 836, 1351
EcoT14I	CCWWGG	3 cut(s)	177, 577, 750
EcoT38I	GRGCYC	1 cut(s)	817
ErhI	CCWWGG	3 cut(s)	177, 577, 750
Esp3I	CGTCTC	1 cut(s)	1137
FaeI	CATG	6 cut(s)	407, 436, 581, 813, 907, 1081
FalI	AAGNNNNNCTT	2 cut(s)	720, 752
FaqI	GGGAC	2 cut(s)	706, 988
FatI	CATG	6 cut(s)	403, 432, 577, 809, 903, 1077
FbaI	TGATCA	1 cut(s)	1074
FblI	GTMKAC	1 cut(s)	1320
Fnu4HI	GCNGC	8 cut(s)	119, 135, 785, 1106, 1286, 1289, 1357, 1379
FokI	GGATG	6 cut(s)	32, 400, 964, 1110, 1134, 1340
FriOI	GRGCYC	1 cut(s)	817
Fsp4HI	GCNGC	8 cut(s)	119, 135, 785, 1106, 1286, 1289, 1357, 1379
FspBI	CTAG	2 cut(s)	917, 1424
GlaI	GCGC	2 cut(s)	69, 1061
GluI	GCNGC	8 cut(s)	119, 135, 785, 1106, 1286, 1289, 1357, 1379
GsuI	CTGGAG	3 cut(s)	267, 666, 1256
HaeII	RGCGCY	1 cut(s)	71
HaeIII	GGCC	3 cut(s)	576, 1222, 1260
HapII	CCGG	3 cut(s)	48, 1365, 1398
HgaI	GACGC	3 cut(s)	1051, 1154, 1390
HhaI	GCGC	2 cut(s)	70, 1062
Hin1I	GRCGYC	2 cut(s)	1146, 1401
Hin1II	CATG	6 cut(s)	407, 436, 581, 813, 907, 1081
Hin6I	GCGC	2 cut(s)	68, 1060
HinP1I	GCGC	2 cut(s)	68, 1060
HincII	GTYRAC	1 cut(s)	1321
HindII	GTYRAC	1 cut(s)	1321
HindIII	AAGCTT	1 cut(s)	194
HinfI	GANTC	4 cut(s)	513, 582, 1134, 1233
HpaII	CCGG	3 cut(s)	48, 1365, 1398
HphI	GGTGA	6 cut(s)	37, 262, 296, 634, 1292, 1421
Hpy166II	GTNNAC	4 cut(s)	430, 487, 521, 1321
Hpy188I	TCNGA	3 cut(s)	35, 112, 1406
Hpy8I	GTNNAC	4 cut(s)	430, 487, 521, 1321
Hpy99I	CGWCG	2 cut(s)	1325, 1406
HpyAV	CCTTC	5 cut(s)	107, 401, 615, 1047, 1328
HpyCH4III	ACNGT	6 cut(s)	307, 448, 603, 880, 1168, 1180
HpyCH4IV	ACGT	2 cut(s)	843, 1041
HpyCH4V	TGCA	6 cut(s)	134, 290, 784, 898, 950, 1105
HpyF10VI	GCNNNNNNNGC	6 cut(s)	53, 164, 173, 890, 1228, 1282
HpyF3I	CTNAG	5 cut(s)	490, 772, 934, 958, 1393
HpySE526I	ACGT	2 cut(s)	843, 1041
Hsp92I	GRCGYC	2 cut(s)	1146, 1401
Hsp92II	CATG	6 cut(s)	407, 436, 581, 813, 907, 1081
HspAI	GCGC	2 cut(s)	68, 1060
KpnI	GGTACC	2 cut(s)	835, 1189
KroI	GCCGGC	1 cut(s)	1397
KroNI	GCCGGC	1 cut(s)	1399
Ksp22I	TGATCA	1 cut(s)	1074
LguI	GCTCTTC	1 cut(s)	1377
LmnI	GCTCC	1 cut(s)	820
Lsp1109I	GCAGC	8 cut(s)	105, 146, 796, 1117, 1272, 1275, 1368, 1390
LweI	GCATC	4 cut(s)	163, 277, 880, 937
MaeI	CTAG	2 cut(s)	917, 1424
MaeII	ACGT	2 cut(s)	843, 1041
MaeIII	GTNAC	5 cut(s)	229, 476, 498, 1063, 1409
MboII	GAAGA	9 cut(s)	85, 363, 658, 719, 728, 956, 1058, 1358, 1364
MflI	RGATCY	7 cut(s)	378, 654, 709, 793, 1115, 1348, 1419
MhlI	GDGCHC	4 cut(s)	8, 169, 817, 1372
MluCI	AATT	4 cut(s)	223, 344, 742, 1191
MlyI	GAGTC	3 cut(s)	507, 576, 1242
MmeI	TCCRAC	4 cut(s)	13, 81, 274, 1384
MroNI	GCCGGC	1 cut(s)	1397
MseI	TTAA	3 cut(s)	453, 806, 860
MslI	CAYNNNNRTG	3 cut(s)	11, 808, 1305
MspA1I	CMGCKG	2 cut(s)	62, 545
MspCI	CTTAAG	1 cut(s)	859
MspI	CCGG	3 cut(s)	48, 1365, 1398
MspR9I	CCNGG	5 cut(s)	49, 160, 701, 838, 1353
MvaI	CCWGG	4 cut(s)	160, 701, 838, 1353
MvnI	CGCG	2 cut(s)	1062, 1295
MwoI	GCNNNNNNNGC	6 cut(s)	53, 164, 173, 890, 1228, 1282
NaeI	GCCGGC	1 cut(s)	1399
NciI	CCSGG	1 cut(s)	49
NcoI	CCATGG	1 cut(s)	577
NgoMIV	GCCGGC	1 cut(s)	1397
NlaIII	CATG	6 cut(s)	407, 436, 581, 813, 907, 1081
NlaIV	GGNNCC	5 cut(s)	694, 816, 833, 1187, 1342
NmuCI	GTSAC	4 cut(s)	476, 498, 1063, 1409
OliI	CACNNNNGTG	1 cut(s)	11
PaeR7I	CTCGAG	1 cut(s)	326
PciSI	GCTCTTC	1 cut(s)	1377
PdiI	GCCGGC	1 cut(s)	1399
PfeI	GAWTC	1 cut(s)	1134
PinAI	ACCGGT	1 cut(s)	1364
PkrI	GCNGC	8 cut(s)	120, 136, 786, 1107, 1287, 1290, 1358, 1380
PleI	GAGTC	3 cut(s)	507, 576, 1241
PpsI	GAGTC	3 cut(s)	507, 576, 1241
Ppu21I	YACGTR	1 cut(s)	1042
PpuMI	RGGWCCY	2 cut(s)	693, 754
Psp5II	RGGWCCY	2 cut(s)	693, 754
Psp6I	CCWGG	4 cut(s)	158, 699, 836, 1351
PspGI	CCWGG	4 cut(s)	158, 699, 836, 1351
PspN4I	GGNNCC	5 cut(s)	694, 816, 833, 1187, 1342
PspPI	GGNCC	5 cut(s)	296, 693, 754, 1083, 1297
PspPPI	RGGWCCY	2 cut(s)	693, 754
PspXI	VCTCGAGB	1 cut(s)	326
PsuI	RGATCY	7 cut(s)	378, 654, 709, 793, 1115, 1348, 1419
PvuII	CAGCTG	1 cut(s)	545
RsaI	GTAC	7 cut(s)	309, 735, 833, 987, 1002, 1029, 1187
RsaNI	GTAC	7 cut(s)	308, 734, 832, 986, 1001, 1028, 1186
RseI	CAYNNNNRTG	3 cut(s)	11, 808, 1305
SalI	GTCGAC	1 cut(s)	1319
SapI	GCTCTTC	1 cut(s)	1377
SaqAI	TTAA	3 cut(s)	453, 806, 860
SatI	GCNGC	8 cut(s)	119, 135, 785, 1106, 1286, 1289, 1357, 1379
Sau96I	GGNCC	5 cut(s)	296, 693, 754, 1083, 1297
ScaI	AGTACT	3 cut(s)	735, 1002, 1029
SchI	GAGTC	3 cut(s)	507, 576, 1242
ScrFI	CCNGG	5 cut(s)	49, 160, 701, 838, 1353
SduI	GDGCHC	4 cut(s)	8, 169, 817, 1372
SfaNI	GCATC	4 cut(s)	163, 277, 880, 937
Sfr274I	CTCGAG	1 cut(s)	326
SinI	GGWCC	5 cut(s)	296, 693, 754, 1083, 1297
SlaI	CTCGAG	1 cut(s)	326
SmiMI	CAYNNNNRTG	3 cut(s)	11, 808, 1305
SmlI	CTYRAG	6 cut(s)	218, 326, 570, 737, 859, 1138
SmoI	CTYRAG	6 cut(s)	218, 326, 570, 737, 859, 1138
SnaBI	TACGTA	1 cut(s)	1042
Sse9I	AATT	4 cut(s)	223, 344, 742, 1191
SsiI	CCGC	2 cut(s)	62, 1295
SspMI	CTAG	2 cut(s)	917, 1424
StyD4I	CCNGG	5 cut(s)	47, 158, 699, 836, 1351
StyI	CCWWGG	3 cut(s)	177, 577, 750
TaaI	ACNGT	6 cut(s)	307, 448, 603, 880, 1168, 1180
TaiI	ACGT	2 cut(s)	846, 1044
TaqII	GACCGA	1 cut(s)	313
TasI	AATT	4 cut(s)	223, 344, 742, 1191
TatI	WGTACW	5 cut(s)	307, 733, 985, 1000, 1027
TfiI	GAWTC	1 cut(s)	1134
Tru1I	TTAA	3 cut(s)	453, 806, 860
Tru9I	TTAA	3 cut(s)	453, 806, 860
TscAI	CASTG	3 cut(s)	133, 498, 957
TseFI	GTSAC	4 cut(s)	476, 498, 1063, 1409
TseI	GCWGC	8 cut(s)	118, 134, 784, 1105, 1285, 1288, 1356, 1378
Tsp45I	GTSAC	4 cut(s)	476, 498, 1063, 1409
TspDTI	ATGAA	5 cut(s)	344, 357, 402, 420, 1188
TspGWI	ACGGA	3 cut(s)	353, 506, 807
TspRI	CASTG	3 cut(s)	133, 498, 957
Vha464I	CTTAAG	1 cut(s)	859
VpaK11BI	GGWCC	5 cut(s)	296, 693, 754, 1083, 1297
XagI	CCTNNNNNAGG	1 cut(s)	915
XcmI	CCANNNNNNNNNTGG	1 cut(s)	430
XhoI	CTCGAG	1 cut(s)	326
XmiI	GTMKAC	1 cut(s)	1320
XspI	CTAG	2 cut(s)	917, 1424
ZrmI	AGTACT	3 cut(s)	735, 1002, 1029

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.