Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0001290

Physical Location & Seq

Genomic Coordinates Forward (+)

33715314 .. 33717614

Gene Structure 2301 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1231700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1221 bp

ATGATGGCAAGAGTGGAGGATGTGGTGATAGTTGGGGCAGGAATAGCAGGGCTGGCGACTGCCGTGGCGCTCAAGAGAGCTGGAGTTGAAGCGTTGGTATTGGAGAAATCAGACGGCCTCAGAGCCACCGGTGCAGCTTTAACCCTCTTCCCCAATGCTTGGTGTGCCCTTGATGCCTTGGGTGTTTCCCAGCACCTCACTTCTCTTTATGCTCCAATCAAAAAGGGGTATGTAACTGACATTGATACCGGAGAAATCCAAGAAGTTTCTTTCGCCGCATCCAATGGAAACGACCCAGTTAGACCAAGGTCAGTAAACCGAAAAGCTTTACTCAAGACTTTGGCAGATGAGTTGCCACCAAATTCAATCCGTTTTGCTTCAAAGCTTACTGCTATCGAGACCCAAGAACACGAAGGCTCGTCCATTTCAGTTATTCACATGGGAGATGGGACAATAATCAAAGCAAAGATTCTGATAGGCTGCGATGGGATTCACTCAGTGGTTGCCCGCTGGTTAGGACTTGCCGAACCAGTGTATTCAGGTCGATGGGCAGTTCGTGGCTTGGCTGTGTTTCCTGAAGGCCACCGATTGGACTATAACGTGCAGCAATATGTAGGACTTAACAGAAGGGCTGGTTTTGTTCCCCTCAACGACAAAGAGATCTATTGGTTCTTTGGCACATCTCCGGCTAAAGGGACAGATCTTGGGGATGAGCCGGAAGTTATAAGACAAGAAGTGATTGAGAATTATGCCAAGGACCTTCCTCCTATATACCTAGACATTGTGCAGCACTCTGATCTTTCAACATTATCATGGGCTCCGTTAATGTTCAGGTACCCGTGGAATGTAGTATTCGGGAACCTAGGGAAGCAAAACATCACGGTGGCTGGTGATGCCATGCACCCCATGACACCTGATTTAGGACAAGGAGGTTGCTTGGCATTGGAAGATGCCGTGGTCTTGGGTAGATACATAGGGACATCATTTGTACAAAATGGACGACTCGTGCCAAAAGAGATAGATAGTGCGATAGGGAAATACGTGGAAGAAAGAAGGTGGCGTGTCGCGCTGTTGACTGCAGGATCATATTTATCCGGATGGGTGCAGCATTTAGGGCCTGGATGGGTGAGGAAGTTCTTGAGGGATGCCATATTTTACAGGTTTATTTTCACTAAACTTGTTAAACTTTCACATTATGATTGTGGAAAACTTGATTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006082 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009266 GO:0009314 GO:0009408 GO:0009414 GO:0009415 GO:0009416 GO:0009507 GO:0009526 GO:0009536 GO:0009540 GO:0009628 GO:0009639 GO:0009687 GO:0009688 GO:0009743 GO:0009756 GO:0009941 GO:0009987 GO:0010033 GO:0010035 GO:0010114 GO:0010182 GO:0016053 GO:0016106 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0016122 GO:0016123 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0019752 GO:0023052 GO:0030104 GO:0031967 GO:0031975 GO:0032501 GO:0032787 GO:0042221 GO:0042440 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043288 GO:0043289 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0046148 GO:0046165 GO:0046394 GO:0048871 GO:0048878 GO:0050789 GO:0050794 GO:0050878 GO:0050891 GO:0050896 GO:0051716 GO:0052662 GO:0055114 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071704 GO:0072330 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1901701 GO:1902644 GO:1902645

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD_binding_3

Protein Analysis

407

Amino Acids

44.54

Weight (kDa)

6.76

Isoelectric Point (pI)

36.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_3	PF01494	7 - 327	3e-25	FAD binding domain
DAO	PF01266	7 - 54	2.8e-08	FAD dependent oxidoreductase
NAD_binding_8	PF13450	10 - 40	1.1e-06	NAD(P)-binding Rossmann-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001290)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g23740 FvH4_1g23750 FvH4_1g23770
malus_domestica	MD11G1231700.v1.1 MD11G1231900.v1.1
prunus_persica	Prupe.6G162100_v2.0.a1
pyrus_communis	pycom11g20520
rosa_chinensis	RchiOBHm_Chr2g0119591 RchiOBHm_Chr2g0119621 RchiOBHm_Chr2g0119641 RchiOBHm_Chr5g0013091
rosa_laevigata	RLG00000018140 RLG00000018142 RLG00000018474 RLG00000018477 RLG00000018479 RLG00000018481 RLG00000018482 RLG00000031985
rosa_multiflora	Rmu_sc0000825.1_g000006 Rmu_sc0000825.1_g000015 Rmu_sc0003158.1_g000005 Rmu_sc0003892.1_g000012 Rmu_sc0027906.1_g000001 Rmu_sc0040685.1_g000001
rosa_roxburghii	Rroxscaffold_1G00063490 Rroxscaffold_2G00124140 Rroxscaffold_2G00124230 Rroxscaffold_2G00124240 Rroxscaffold_2G00124250
rosa_rugosa	Rorug02G0222300 Rorug02G0222400 Rorug02G0222400 Rorug02G0222400 Rorug02G0222500.1 Rorug02G0223100 Rorug05G0007200
rosa_samantha	Rh2AG279800 Rh2AG280300 Rh2BG291500 Rh2BG291900 Rh2DG286500 Rh2DG286900 Rh2DG287300 Rh2DG305700 Rh2DG305900 Rh2DG306000 Rh5AG101300 Rh5BG097800 Rh5CG109900 Rh5DG096900
rosa_wichuraiana	Rw2G022360 Rw2G022400 Rw2G022410 Rw5G008850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	725
Acc65I	GGTACC	1 cut(s)	834
AccB1I	GGYRCC	1 cut(s)	834
AccB7I	CCANNNNNTGG	2 cut(s)	159, 589
AccII	CGCG	1 cut(s)	1067
AccIII	TCCGGA	1 cut(s)	1094
AciI	CCGC	2 cut(s)	276, 508
AclWI	GGATC	1 cut(s)	1090
AcsI	RAATTY	1 cut(s)	361
AcuI	CTGAAG	1 cut(s)	597
AdeI	CACNNNGTG	1 cut(s)	499
AfaI	GTAC	2 cut(s)	836, 990
AfiI	CCNNNNNNNGG	4 cut(s)	159, 589, 692, 920
AgeI	ACCGGT	1 cut(s)	128
AgsI	TTSAA	4 cut(s)	89, 366, 381, 804
AjnI	CCWGG	1 cut(s)	1117
AluBI	AGCT	4 cut(s)	80, 137, 326, 385
AluI	AGCT	4 cut(s)	80, 137, 326, 385
Alw26I	GTCTC	1 cut(s)	392
AlwI	GGATC	1 cut(s)	1090
Aor13HI	TCCGGA	1 cut(s)	1094
AoxI	GGCC	3 cut(s)	115, 580, 1115
ApeKI	GCWGC	5 cut(s)	134, 480, 604, 787, 1105
ApoI	RAATTY	1 cut(s)	361
AsiGI	ACCGGT	1 cut(s)	128
Asp718I	GGTACC	1 cut(s)	834
AspA2I	CCTAGG	1 cut(s)	862
AspLEI	GCGC	2 cut(s)	70, 1069
AspS9I	GGNCC	2 cut(s)	757, 1115
AsuHPI	GGTGA	3 cut(s)	37, 902, 1138
AvaII	GGWCC	1 cut(s)	757
AvrII	CCTAGG	1 cut(s)	862
BaeGI	GKGCMC	1 cut(s)	169
BanI	GGYRCC	1 cut(s)	834
BanII	GRGCYC	1 cut(s)	820
BauI	CACGAG	1 cut(s)	1004
BbvI	GCAGC	5 cut(s)	146, 467, 616, 799, 1117
BccI	CCATC	5 cut(s)	440, 479, 540, 1092, 1116
BceAI	ACGGC	3 cut(s)	47, 130, 938
BciT130I	CCWGG	1 cut(s)	1119
BcoDI	GTCTC	1 cut(s)	392
BfaI	CTAG	3 cut(s)	776, 863, 1219
BfmI	CTRYAG	1 cut(s)	1077
BfoI	RGCGCY	1 cut(s)	71
BglII	AGATCT	2 cut(s)	660, 700
BisI	GCNGC	6 cut(s)	135, 276, 481, 605, 788, 1106
BlnI	CCTAGG	1 cut(s)	862
BlsI	GCNGC	6 cut(s)	136, 277, 482, 606, 789, 1107
Bme1390I	CCNGG	1 cut(s)	1119
Bme18I	GGWCC	1 cut(s)	757
BmgT120I	GGNCC	2 cut(s)	757, 1115
BmiI	GGNNCC	3 cut(s)	819, 836, 860
BmrFI	CCNGG	1 cut(s)	1119
BmrI	ACTGGG	1 cut(s)	290
BmsI	GCATC	5 cut(s)	163, 287, 883, 940, 1135
BmuI	ACTGGG	1 cut(s)	290
BoxI	GACNNNNGTC	1 cut(s)	307
BpmI	CTGGAG	1 cut(s)	102
BpuEI	CTTGAG	3 cut(s)	56, 317, 1159
BsaAI	YACGTR	1 cut(s)	1042
BsaI	GGTCTC	1 cut(s)	392
BsaJI	CCNNGG	7 cut(s)	63, 177, 305, 753, 839, 862, 954
BsaWI	WCCGGW	3 cut(s)	128, 248, 1094
Bsc4I	CCNNNNNNNGG	4 cut(s)	159, 589, 692, 920
Bse118I	RCCGGY	1 cut(s)	128
Bse1I	ACTGG	2 cut(s)	296, 530
BseAI	TCCGGA	1 cut(s)	1094
BseBI	CCWGG	1 cut(s)	1119
BseDI	CCNNGG	7 cut(s)	63, 177, 305, 753, 839, 862, 954
BseGI	GGATG	6 cut(s)	25, 278, 715, 1103, 1127, 1150
BseLI	CCNNNNNNNGG	4 cut(s)	159, 589, 692, 920
BseMII	CTCAG	2 cut(s)	133, 510
BseNI	ACTGG	2 cut(s)	296, 530
BseSI	GKGCMC	1 cut(s)	169
BseXI	GCAGC	5 cut(s)	146, 467, 616, 799, 1117
BseYI	CCCAGC	1 cut(s)	189
BsgI	GTGCAG	4 cut(s)	153, 623, 806, 1124
Bsh1236I	CGCG	1 cut(s)	1067
BshFI	GGCC	3 cut(s)	117, 582, 1117
BshNI	GGYRCC	1 cut(s)	834
BshTI	ACCGGT	1 cut(s)	128
BsiSI	CCGG	5 cut(s)	129, 249, 686, 716, 1095
BslFI	GGGAC	3 cut(s)	463, 709, 991
BslI	CCNNNNNNNGG	4 cut(s)	159, 589, 692, 920
BsmAI	GTCTC	1 cut(s)	392
BsmFI	GGGAC	3 cut(s)	463, 709, 991
BsnI	GGCC	3 cut(s)	117, 582, 1117
Bso31I	GGTCTC	1 cut(s)	392
Bsp1286I	GDGCHC	2 cut(s)	169, 820
Bsp13I	TCCGGA	1 cut(s)	1094
Bsp1407I	TGTACA	1 cut(s)	988
Bsp143I	GATC	4 cut(s)	660, 700, 796, 1082
BspACI	CCGC	2 cut(s)	276, 508
BspANI	GGCC	3 cut(s)	117, 582, 1117
BspCNI	CTCAG	2 cut(s)	132, 509
BspEI	TCCGGA	1 cut(s)	1094
BspFNI	CGCG	1 cut(s)	1067
BspLI	GGNNCC	3 cut(s)	819, 836, 860
BspMAI	CTGCAG	1 cut(s)	1081
BspPI	GGATC	1 cut(s)	1090
BspT107I	GGYRCC	1 cut(s)	834
BspTNI	GGTCTC	1 cut(s)	392
BsrFI	RCCGGY	1 cut(s)	128
BsrGI	TGTACA	1 cut(s)	988
BsrI	ACTGG	2 cut(s)	296, 530
BssAI	RCCGGY	1 cut(s)	128
BssECI	CCNNGG	7 cut(s)	63, 177, 305, 753, 839, 862, 954
BssMI	GATC	4 cut(s)	660, 700, 796, 1082
BssSI	CACGAG	1 cut(s)	1004
BssT1I	CCWWGG	4 cut(s)	177, 305, 753, 862
Bst2BI	CACGAG	1 cut(s)	1004
Bst2UI	CCWGG	1 cut(s)	1119
Bst4CI	ACNGT	1 cut(s)	883
Bst6I	CTCTTC	1 cut(s)	152
BstAUI	TGTACA	1 cut(s)	988
BstBAI	YACGTR	1 cut(s)	1042
BstC8I	GCNNGC	2 cut(s)	54, 508
BstDEI	CTNAG	2 cut(s)	119, 496
BstDSI	CCRYGG	3 cut(s)	63, 839, 954
BstENI	CCTNNNNNAGG	1 cut(s)	918
BstF5I	GGATG	6 cut(s)	25, 278, 715, 1103, 1127, 1150
BstFNI	CGCG	1 cut(s)	1067
BstH2I	RGCGCY	1 cut(s)	71
BstHHI	GCGC	2 cut(s)	70, 1069
BstKTI	GATC	4 cut(s)	663, 703, 799, 1085
BstMAI	GTCTC	1 cut(s)	392
BstMBI	GATC	4 cut(s)	660, 700, 796, 1082
BstMWI	GCNNNNNNNGC	8 cut(s)	44, 53, 131, 164, 173, 893, 1066, 1114
BstNI	CCWGG	1 cut(s)	1119
BstPAI	GACNNNNGTC	1 cut(s)	307
BstSCI	CCNGG	1 cut(s)	1117
BstSFI	CTRYAG	1 cut(s)	1077
BstSLI	GKGCMC	1 cut(s)	169
BstUI	CGCG	1 cut(s)	1067
BstV1I	GCAGC	5 cut(s)	146, 467, 616, 799, 1117
BstX2I	RGATCY	2 cut(s)	660, 700
BstYI	RGATCY	2 cut(s)	660, 700
BsuRI	GGCC	3 cut(s)	117, 582, 1117
BtgI	CCRYGG	3 cut(s)	63, 839, 954
BtgZI	GCGATG	1 cut(s)	498
BtsCI	GGATG	6 cut(s)	25, 278, 715, 1103, 1127, 1150
BtsIMutI	CAGTG	2 cut(s)	504, 537
Cac8I	GCNNGC	2 cut(s)	54, 508
CfoI	GCGC	2 cut(s)	70, 1069
Cfr10I	RCCGGY	1 cut(s)	128
Cfr13I	GGNCC	2 cut(s)	757, 1115
Csp6I	GTAC	2 cut(s)	835, 989
CspAI	ACCGGT	1 cut(s)	128
CviAII	CATG	4 cut(s)	439, 813, 898, 907
CviQI	GTAC	2 cut(s)	835, 989
DdeI	CTNAG	2 cut(s)	119, 496
DpnI	GATC	4 cut(s)	662, 702, 798, 1084
DpnII	GATC	4 cut(s)	660, 700, 796, 1082
DraIII	CACNNNGTG	1 cut(s)	499
Eam1104I	CTCTTC	1 cut(s)	152
EarI	CTCTTC	1 cut(s)	152
Eco130I	CCWWGG	4 cut(s)	177, 305, 753, 862
Eco24I	GRGCYC	1 cut(s)	820
Eco31I	GGTCTC	1 cut(s)	392
Eco47I	GGWCC	1 cut(s)	757
Eco57I	CTGAAG	1 cut(s)	597
EcoNI	CCTNNNNNAGG	1 cut(s)	918
EcoO109I	RGGNCCY	2 cut(s)	757, 1115
EcoRII	CCWGG	1 cut(s)	1117
EcoT14I	CCWWGG	4 cut(s)	177, 305, 753, 862
EcoT38I	GRGCYC	1 cut(s)	820
ErhI	CCWWGG	4 cut(s)	177, 305, 753, 862
FaeI	CATG	4 cut(s)	442, 816, 901, 910
FaqI	GGGAC	3 cut(s)	463, 709, 991
FatI	CATG	4 cut(s)	438, 812, 897, 906
FauI	CCCGC	1 cut(s)	515
Fnu4HI	GCNGC	6 cut(s)	135, 276, 481, 605, 788, 1106
FokI	GGATG	6 cut(s)	32, 265, 722, 1110, 1134, 1157
FriOI	GRGCYC	1 cut(s)	820
Fsp4HI	GCNGC	6 cut(s)	135, 276, 481, 605, 788, 1106
FspBI	CTAG	3 cut(s)	776, 863, 1219
GlaI	GCGC	2 cut(s)	69, 1068
GluI	GCNGC	6 cut(s)	135, 276, 481, 605, 788, 1106
GsaI	CCCAGC	1 cut(s)	193
GsuI	CTGGAG	1 cut(s)	102
HaeII	RGCGCY	1 cut(s)	71
HaeIII	GGCC	3 cut(s)	117, 582, 1117
HapII	CCGG	5 cut(s)	129, 249, 686, 716, 1095
HhaI	GCGC	2 cut(s)	70, 1069
Hin1II	CATG	4 cut(s)	442, 816, 901, 910
Hin6I	GCGC	2 cut(s)	68, 1067
HinP1I	GCGC	2 cut(s)	68, 1067
HincII	GTYRAC	1 cut(s)	1074
HindII	GTYRAC	1 cut(s)	1074
HindIII	AAGCTT	2 cut(s)	324, 383
HinfI	GANTC	3 cut(s)	469, 490, 1002
HpaII	CCGG	5 cut(s)	129, 249, 686, 716, 1095
HphI	GGTGA	3 cut(s)	37, 902, 1138
Hpy166II	GTNNAC	2 cut(s)	316, 1074
Hpy188I	TCNGA	4 cut(s)	112, 122, 474, 796
Hpy188III	TCNNGA	7 cut(s)	73, 334, 397, 575, 856, 1095, 1138
Hpy8I	GTNNAC	2 cut(s)	316, 1074
HpyAV	CCTTC	5 cut(s)	407, 572, 621, 770, 1047
HpyCH4III	ACNGT	1 cut(s)	883
HpyCH4IV	ACGT	2 cut(s)	600, 1041
HpyCH4V	TGCA	6 cut(s)	134, 604, 787, 901, 1079, 1105
HpyF10VI	GCNNNNNNNGC	8 cut(s)	44, 53, 131, 164, 173, 893, 1066, 1114
HpyF3I	CTNAG	2 cut(s)	119, 496
HpySE526I	ACGT	2 cut(s)	600, 1041
Hsp92II	CATG	4 cut(s)	442, 816, 901, 910
HspAI	GCGC	2 cut(s)	68, 1067
Kpn2I	TCCGGA	1 cut(s)	1094
KpnI	GGTACC	1 cut(s)	838
Kzo9I	GATC	4 cut(s)	660, 700, 796, 1082
LmnI	GCTCC	2 cut(s)	217, 823
Lsp1109I	GCAGC	5 cut(s)	146, 467, 616, 799, 1117
LweI	GCATC	5 cut(s)	163, 287, 883, 940, 1135
MaeI	CTAG	3 cut(s)	776, 863, 1219
MaeII	ACGT	2 cut(s)	600, 1041
MaeIII	GTNAC	1 cut(s)	232
MalI	GATC	4 cut(s)	662, 702, 798, 1084
MboI	GATC	4 cut(s)	660, 700, 796, 1082
MboII	GAAGA	3 cut(s)	139, 959, 1058
MflI	RGATCY	2 cut(s)	660, 700
MhlI	GDGCHC	2 cut(s)	169, 820
MluCI	AATT	2 cut(s)	361, 745
MlyI	GAGTC	1 cut(s)	996
MnlI	CCTC	9 cut(s)	10, 128, 155, 206, 656, 774, 923, 1122, 1134
MroI	TCCGGA	1 cut(s)	1094
MseI	TTAA	4 cut(s)	140, 621, 824, 1182
MslI	CAYNNNNRTG	2 cut(s)	811, 881
MspA1I	CMGCKG	1 cut(s)	510
MspI	CCGG	5 cut(s)	129, 249, 686, 716, 1095
MspR9I	CCNGG	1 cut(s)	1119
MvaI	CCWGG	1 cut(s)	1119
MvnI	CGCG	1 cut(s)	1067
MwoI	GCNNNNNNNGC	8 cut(s)	44, 53, 131, 164, 173, 893, 1066, 1114
NdeII	GATC	4 cut(s)	660, 700, 796, 1082
NlaIII	CATG	4 cut(s)	442, 816, 901, 910
NlaIV	GGNNCC	3 cut(s)	819, 836, 860
PfeI	GAWTC	2 cut(s)	469, 490
PflMI	CCANNNNNTGG	2 cut(s)	159, 589
PinAI	ACCGGT	1 cut(s)	128
PkrI	GCNGC	6 cut(s)	136, 277, 482, 606, 789, 1107
PleI	GAGTC	1 cut(s)	996
PpsI	GAGTC	1 cut(s)	996
Ppu21I	YACGTR	1 cut(s)	1042
PpuMI	RGGWCCY	1 cut(s)	757
PshAI	GACNNNNGTC	1 cut(s)	307
PsiI	TTATAA	1 cut(s)	725
Psp5II	RGGWCCY	1 cut(s)	757
Psp6I	CCWGG	1 cut(s)	1117
PspFI	CCCAGC	1 cut(s)	189
PspGI	CCWGG	1 cut(s)	1117
PspN4I	GGNNCC	3 cut(s)	819, 836, 860
PspPI	GGNCC	2 cut(s)	757, 1115
PspPPI	RGGWCCY	1 cut(s)	757
PstI	CTGCAG	1 cut(s)	1081
PsuI	RGATCY	2 cut(s)	660, 700
RsaI	GTAC	2 cut(s)	836, 990
RsaNI	GTAC	2 cut(s)	835, 989
RseI	CAYNNNNRTG	2 cut(s)	811, 881
SaqAI	TTAA	4 cut(s)	140, 621, 824, 1182
SatI	GCNGC	6 cut(s)	135, 276, 481, 605, 788, 1106
Sau3AI	GATC	4 cut(s)	660, 700, 796, 1082
Sau96I	GGNCC	2 cut(s)	757, 1115
SchI	GAGTC	1 cut(s)	996
ScrFI	CCNGG	1 cut(s)	1119
SduI	GDGCHC	2 cut(s)	169, 820
SfaNI	GCATC	5 cut(s)	163, 287, 883, 940, 1135
SfcI	CTRYAG	1 cut(s)	1077
SgrAI	CRCCGGYG	1 cut(s)	128
SinI	GGWCC	1 cut(s)	757
SmiMI	CAYNNNNRTG	2 cut(s)	811, 881
SmlI	CTYRAG	3 cut(s)	71, 332, 1138
SmoI	CTYRAG	3 cut(s)	71, 332, 1138
Sse9I	AATT	2 cut(s)	361, 745
SsiI	CCGC	2 cut(s)	276, 508
SspMI	CTAG	3 cut(s)	776, 863, 1219
StyD4I	CCNGG	1 cut(s)	1117
StyI	CCWWGG	4 cut(s)	177, 305, 753, 862
TaaI	ACNGT	1 cut(s)	883
TaiI	ACGT	2 cut(s)	603, 1044
TaqI	TCGA	2 cut(s)	396, 544
TasI	AATT	2 cut(s)	361, 745
TatI	WGTACW	1 cut(s)	988
TauI	GCSGC	1 cut(s)	278
TfiI	GAWTC	2 cut(s)	469, 490
Tru1I	TTAA	4 cut(s)	140, 621, 824, 1182
Tru9I	TTAA	4 cut(s)	140, 621, 824, 1182
TscAI	CASTG	2 cut(s)	504, 537
TseI	GCWGC	5 cut(s)	134, 480, 604, 787, 1105
TspGWI	ACGGA	2 cut(s)	359, 810
TspRI	CASTG	2 cut(s)	504, 537
Van91I	CCANNNNNTGG	2 cut(s)	159, 589
VpaK11BI	GGWCC	1 cut(s)	757
XagI	CCTNNNNNAGG	1 cut(s)	918
XapI	RAATTY	1 cut(s)	361
XmaJI	CCTAGG	1 cut(s)	862
XspI	CTAG	3 cut(s)	776, 863, 1219

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.