Rh5AG101200

DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0007782
Physical Location & Seq
Forward (+)
9358694 .. 9361297
2604 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG101200.1

Sequence Viewer

Length: 357 bp
ATGCAGGAGCTTATGGCTCAACAAGGCGCTGGAGGGGCCAGAGGGCAGCAAAACCCTGAACAGCAGAAGGCAAATGATGATGCCAAGAGGGAGGCAGAAGAACGGAGGCAGATGATGCTTAGTCAGATTTTGTCATCTGAAGCTCGAGAAAGACTTGCTCGAATTGCTTTGGTGAAACCTGAGAAGGCAAGAGGTGTTGAAGATGTCATACTGAGAGCTGCTCAAATGGGCCAAATAGCTGAGAAGGTTACCGAGGAGAGGCTTATTTCATTGTTGGAACAGATCAACAACCAAACAACCAAGCAGACTAAAGTCACTATTCAGAGGTGTCGGAATGTTCTTGAAGATGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005539 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006915 GO:0008047 GO:0008092 GO:0008150 GO:0008201 GO:0008219 GO:0008285 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010604 GO:0010628 GO:0010638 GO:0010646 GO:0010647 GO:0010698 GO:0010821 GO:0010822 GO:0010941 GO:0010942 GO:0010950 GO:0010952 GO:0012501 GO:0015631 GO:0019222 GO:0023051 GO:0023056 GO:0030162 GO:0030234 GO:0031323 GO:0031325 GO:0032268 GO:0032270 GO:0032386 GO:0032388 GO:0032879 GO:0032880 GO:0033043 GO:0033157 GO:0042127 GO:0042221 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043280 GO:0043281 GO:0044093 GO:0044424 GO:0044444 GO:0044464 GO:0045862 GO:0048487 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051222 GO:0051223 GO:0051246 GO:0051247 GO:0051336 GO:0051345 GO:0051716 GO:0052547 GO:0052548 GO:0060255 GO:0060341 GO:0065007 GO:0065009 GO:0070201 GO:0070848 GO:0070887 GO:0071310 GO:0071363 GO:0071495 GO:0071559 GO:0071560 GO:0080090 GO:0090087 GO:0090199 GO:0090200 GO:0090316 GO:0097367 GO:0098772 GO:1901681 GO:1903214 GO:1903332 GO:1903333 GO:1903533 GO:1903636 GO:1903638 GO:1903644 GO:1903645 GO:1903747 GO:1903749 GO:1903827 GO:1903829 GO:1903955 GO:1904589 GO:1904591 GO:1904951 GO:1905475 GO:1905477 GO:2000116 GO:2001056 GO:2001233 GO:2001235
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsDNA_bind

Protein Analysis

118

Amino Acids

13.37

Weight (kDa)

5.65

Isoelectric Point (pI)

48.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
dsDNA_bind PF01984 1 - 109 9.9e-30 Double-stranded DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 159
AfiI CCNNNNNNNGG 1 cut(s) 258
AgsI TTSAA 2 cut(s) 200, 344
AluBI AGCT 4 cut(s) 10, 143, 218, 239
AluI AGCT 4 cut(s) 10, 143, 218, 239
Ama87I CYCGRG 1 cut(s) 144
AoxI GGCC 2 cut(s) 36, 229
ApeKI GCWGC 2 cut(s) 46, 218
AspLEI GCGC 1 cut(s) 29
AspS9I GGNCC 2 cut(s) 36, 229
AsuHPI GGTGA 1 cut(s) 184
AvaI CYCGRG 1 cut(s) 144
BarI GAAGNNNNNNTAC 2 cut(s) 192, 224
BbvI GCAGC 2 cut(s) 58, 205
BfoI RGCGCY 1 cut(s) 30
BisI GCNGC 2 cut(s) 47, 219
BlsI GCNGC 2 cut(s) 48, 220
BmeT110I CYCGRG 1 cut(s) 144
BmgT120I GGNCC 2 cut(s) 36, 229
BmiI GGNNCC 1 cut(s) 37
BmsI GCATC 2 cut(s) 70, 105
BoxI GACNNNNGTC 1 cut(s) 311
BplI GAGNNNNNCTC 2 cut(s) 205, 237
BpmI CTGGAG 1 cut(s) 51
BsaJI CCNNGG 1 cut(s) 252
Bsc4I CCNNNNNNNGG 1 cut(s) 258
BseDI CCNNGG 1 cut(s) 252
BseLI CCNNNNNNNGG 1 cut(s) 258
BseMII CTCAG 3 cut(s) 171, 203, 231
BseRI GAGGAG 1 cut(s) 269
BseXI GCAGC 2 cut(s) 58, 205
BshFI GGCC 2 cut(s) 38, 231
BsiHKCI CYCGRG 1 cut(s) 144
BslI CCNNNNNNNGG 1 cut(s) 258
BsnI GGCC 2 cut(s) 38, 231
BsoBI CYCGRG 1 cut(s) 144
Bsp143I GATC 1 cut(s) 282
BspANI GGCC 2 cut(s) 38, 231
BspCNI CTCAG 3 cut(s) 172, 204, 232
BspLI GGNNCC 1 cut(s) 37
BssECI CCNNGG 1 cut(s) 252
BssMI GATC 1 cut(s) 282
BstAPI GCANNNNNTGC 1 cut(s) 115
BstDEI CTNAG 4 cut(s) 119, 180, 212, 240
BstEII GGTNACC 1 cut(s) 247
BstH2I RGCGCY 1 cut(s) 30
BstHHI GCGC 1 cut(s) 29
BstKTI GATC 1 cut(s) 285
BstMBI GATC 1 cut(s) 282
BstMWI GCNNNNNNNGC 3 cut(s) 35, 115, 164
BstPAI GACNNNNGTC 1 cut(s) 311
BstPI GGTNACC 1 cut(s) 247
BstV1I GCAGC 2 cut(s) 58, 205
BsuRI GGCC 2 cut(s) 38, 231
CfoI GCGC 1 cut(s) 29
Cfr13I GGNCC 2 cut(s) 36, 229
CviJI RGCY 8 cut(s) 10, 17, 38, 143, 218, 231, 239, 262
CviKI_1 RGCY 8 cut(s) 10, 17, 38, 143, 218, 231, 239, 262
DdeI CTNAG 4 cut(s) 119, 180, 212, 240
DpnI GATC 1 cut(s) 284
DpnII GATC 1 cut(s) 282
Eco57I CTGAAG 1 cut(s) 159
Eco88I CYCGRG 1 cut(s) 144
Eco91I GGTNACC 1 cut(s) 247
EcoO65I GGTNACC 1 cut(s) 247
FaiI YATR 2 cut(s) 14, 209
Fnu4HI GCNGC 2 cut(s) 47, 219
Fsp4HI GCNGC 2 cut(s) 47, 219
GlaI GCGC 1 cut(s) 28
GluI GCNGC 2 cut(s) 47, 219
GsuI CTGGAG 1 cut(s) 51
HaeII RGCGCY 1 cut(s) 30
HaeIII GGCC 2 cut(s) 38, 231
HhaI GCGC 1 cut(s) 29
Hin6I GCGC 1 cut(s) 27
HinP1I GCGC 1 cut(s) 27
HphI GGTGA 1 cut(s) 184
Hpy188I TCNGA 4 cut(s) 126, 139, 324, 333
Hpy188III TCNNGA 2 cut(s) 146, 341
HpyAV CCTTC 3 cut(s) 61, 178, 238
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 115, 164
HpyF3I CTNAG 4 cut(s) 119, 180, 212, 240
HspAI GCGC 1 cut(s) 27
Kzo9I GATC 1 cut(s) 282
LmnI GCTCC 1 cut(s) 7
LpnPI CCDG 4 cut(s) 15, 52, 69, 192
Lsp1109I GCAGC 2 cut(s) 58, 205
LweI GCATC 2 cut(s) 70, 105
MaeIII GTNAC 2 cut(s) 247, 313
MalI GATC 1 cut(s) 284
MboI GATC 1 cut(s) 282
MboII GAAGA 3 cut(s) 110, 212, 356
MluCI AATT 1 cut(s) 162
MmeI TCCRAC 2 cut(s) 255, 311
MnlI CCTC 9 cut(s) 26, 35, 81, 85, 99, 185, 247, 252, 318
MwoI GCNNNNNNNGC 3 cut(s) 35, 115, 164
NdeII GATC 1 cut(s) 282
NlaIV GGNNCC 1 cut(s) 37
NmuCI GTSAC 1 cut(s) 313
PaeR7I CTCGAG 1 cut(s) 144
PkrI GCNGC 2 cut(s) 48, 220
PshAI GACNNNNGTC 1 cut(s) 311
PspEI GGTNACC 1 cut(s) 247
PspN4I GGNNCC 1 cut(s) 37
PspPI GGNCC 2 cut(s) 36, 229
SatI GCNGC 2 cut(s) 47, 219
Sau3AI GATC 1 cut(s) 282
Sau96I GGNCC 2 cut(s) 36, 229
SetI ASST 8 cut(s) 12, 145, 181, 196, 220, 241, 249, 329
SfaNI GCATC 2 cut(s) 70, 105
Sfr274I CTCGAG 1 cut(s) 144
SlaI CTCGAG 1 cut(s) 144
SmlI CTYRAG 1 cut(s) 144
SmoI CTYRAG 1 cut(s) 144
Sse9I AATT 1 cut(s) 162
TaqI TCGA 2 cut(s) 145, 160
TasI AATT 1 cut(s) 162
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 2 cut(s) 46, 218
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 1 cut(s) 258
TspGWI ACGGA 1 cut(s) 118
XhoI CTCGAG 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.