MD05G1290600.v1.1

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014427
Physical Location & Seq
Forward (+)
42234625 .. 42238689
4065 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1290600.v1.1.491

Sequence Viewer

Length: 1185 bp
ATGGGAGTGACAATGGGTCTGAATCTGCTGCTGCTGATGGCTATGGTGGCCACCAACATACTCTCACTCTACCACCTTTCCTCCACTCTCCAAACCCCAAAACCACCAACTCCCCAGCCAGTCCCTGACCACCTCCTCCACCAGCTCCACACCATACGCGCCACCATCAACCACCTCACGCGCCACCACCCATCTCCCTCTACCGCCACCTCCAAAACAACCAAACCAACCGTCCCCTCGGATCTCCTCCTCTACTCTCAGCTCTCCCCCATCGCCTCCTCCTGCCACGACCACCCTGACCTCCTCCACAAGTACATGACCTACACCCCCTTCTCTCTCTGCCCGCTGGACTCCGACCTCGCCGAGTCTCTCAGTCTTCGCGGCTGCCACCCGCTCCCCCGCCGCCGCTGCTTTACCAGAACCCCACCAAAACTCACTTCTTCTCTCCCCATGAATCCGTTTCCTCCTTCGCTTCCAGACTCCAGTGTCGTGTGGGCCAAGTATTCCTGCAAAAGCTTCAGCTGCCTCGGCGGCAAAAACCCAAATTTGGGCTTCGACCTGCCTCACGAATTGTCCAACTTCATGACTTACAAGTCGGAGCTCGACCTCCCGATCCCTCAGCTCTTCCAAATCGCCAAAGCAGCCAACTCGGTCCTCCGTCTCGGCATCGACATCGGCGGCGGAACTGGGACTTTCGCGGCGAGGATGAAGCTCTACAATGTCACCATTCTCACGACCACCATGAACCTTGGAATCCCCAACAATGAGGCGGTGGCGCTGAGGGGGTTGGTGCCAATTCACGCTCCGCTGCAGCAGCGGTTGCCGGTGTTCGACGGAGTGGTGGATCTGGTGCGGTGCGGGCACGCCGTGAACCGGTGGATACCCGTAACGGCATTGGAGTTTTTGTTGTTTGATGCGGATAGAGTGTTGAGGGGAGGAGGGTATTTGTGGTTGGATCATTTTTTCAGCAAAGGGGTGGATCTTGAGAAGGTTTTCGGACCGTTGATTGGGAAATTGGGGTACAGGAAGGTGAAGTGGGCTACGGCGAGTAAGAACGATTCCGGTGGGTTGAAGAATGGCGAGGTTTACTTGTCTGCTCTGCTCCAAAAACCCGCATTGCTGCCGATCTTTGCCGTTTACGAATTACCTCTCCTGAGAAGATGGGTGTCCTTCATCGAGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_29

Protein Analysis

395

Amino Acids

43.51

Weight (kDa)

9.32

Isoelectric Point (pI)

47.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29790 AT1G29790
fragaria_vesca FvH4_3g06240 FvH4_3g06240
malus_domestica MD05G1290600.v1.1 MD16G1203100.v1.1
prunus_persica Prupe.4G075100_v2.0.a1
pyrus_communis pycom10g22250
rosa_chinensis RchiOBHm_Chr5g0013071
rosa_laevigata RLG00000031983
rosa_multiflora Rmu_sc0003892.1_g000015
rosa_rugosa Rorug05G0007000
rosa_samantha Rh5AG101000 Rh5BG097600 Rh5CG109700 Rh5DG096600
rosa_wichuraiana Rw5G008830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 485, 592
Acc36I ACCTGC 1 cut(s) 567
AccB1I GGYRCC 1 cut(s) 790
AccBSI CCGCTC 1 cut(s) 394
AccII CGCG 4 cut(s) 159, 181, 381, 698
AclWI GGATC 5 cut(s) 249, 607, 852, 963, 987
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 1 cut(s) 544
AcuI CTGAAG 1 cut(s) 502
AdeI CACNNNGTG 1 cut(s) 868
AfaI GTAC 2 cut(s) 314, 1022
AfiI CCNNNNNNNGG 4 cut(s) 547, 548, 873, 1007
AgeI ACCGGT 1 cut(s) 873
AgsI TTSAA 1 cut(s) 1072
AhdI GACNNNNNGTC 1 cut(s) 15
AjuI GAANNNNNNNTTGG 2 cut(s) 421, 453
AluBI AGCT 7 cut(s) 145, 262, 516, 522, 601, 622, 712
AluI AGCT 7 cut(s) 145, 262, 516, 522, 601, 622, 712
Alw21I GWGCWC 1 cut(s) 603
Alw26I GTCTC 2 cut(s) 372, 665
AlwI GGATC 5 cut(s) 249, 607, 852, 963, 987
AlwNI CAGNNNCTG 1 cut(s) 125
AoxI GGCC 2 cut(s) 48, 495
ApoI RAATTY 1 cut(s) 544
ArsI GACNNNNNNTTYG 2 cut(s) 216, 248
AsiGI ACCGGT 1 cut(s) 873
Asp700I GAANNNNTTC 1 cut(s) 992
AspLEI GCGC 3 cut(s) 161, 183, 778
AspS9I GGNCC 3 cut(s) 495, 652, 998
AsuHPI GGTGA 2 cut(s) 715, 1042
AvaII GGWCC 2 cut(s) 652, 998
BaeGI GKGCMC 1 cut(s) 864
BalI TGGCCA 1 cut(s) 50
BanI GGYRCC 1 cut(s) 790
BanII GRGCYC 1 cut(s) 603
BbsI GAAGAC 1 cut(s) 368
Bbv12I GWGCWC 1 cut(s) 603
BbvCI CCTCAGC 2 cut(s) 618, 779
BccI CCATC 5 cut(s) 31, 173, 199, 278, 1155
BceAI ACGGC 4 cut(s) 851, 906, 1059, 1118
BcgI CGANNNNNNTGC 4 cut(s) 630, 655, 664, 689
BciVI GTATCC 1 cut(s) 873
BcoDI GTCTC 2 cut(s) 372, 665
BfmI CTRYAG 1 cut(s) 809
BfoI RGCGCY 1 cut(s) 779
BfuAI ACCTGC 1 cut(s) 567
BfuI GTATCC 1 cut(s) 873
BglI GCCNNNNNGGC 1 cut(s) 531
Bme18I GGWCC 2 cut(s) 652, 998
BmeRI GACNNNNNGTC 1 cut(s) 15
BmgT120I GGNCC 3 cut(s) 495, 652, 998
BmiI GGNNCC 1 cut(s) 792
BmrI ACTGGG 1 cut(s) 696
BmsI GCATC 2 cut(s) 675, 904
BmuI ACTGGG 1 cut(s) 696
BpiI GAAGAC 1 cut(s) 368
BpmI CTGGAG 1 cut(s) 466
Bpu10I CCTNAGC 2 cut(s) 618, 779
BpuEI CTTGAG 1 cut(s) 1004
BsaJI CCNNGG 3 cut(s) 237, 526, 748
BsaWI WCCGGW 2 cut(s) 873, 1061
BsaXI ACNNNNNCTCC 4 cut(s) 65, 95, 890, 920
Bsc4I CCNNNNNNNGG 4 cut(s) 547, 548, 873, 1007
Bse118I RCCGGY 2 cut(s) 823, 873
Bse1I ACTGG 3 cut(s) 119, 483, 691
Bse3DI GCAATG 1 cut(s) 1115
BseDI CCNNGG 3 cut(s) 237, 526, 748
BseGI GGATG 1 cut(s) 711
BseLI CCNNNNNNNGG 4 cut(s) 547, 548, 873, 1007
BseMI GCAATG 1 cut(s) 1115
BseMII CTCAG 5 cut(s) 272, 385, 632, 770, 1145
BseNI ACTGG 3 cut(s) 119, 483, 691
BseRI GAGGAG 6 cut(s) 125, 236, 239, 268, 293, 951
BseSI GKGCMC 1 cut(s) 864
BseYI CCCAGC 1 cut(s) 114
Bsh1236I CGCG 4 cut(s) 159, 181, 381, 698
BshFI GGCC 2 cut(s) 50, 497
BshNI GGYRCC 1 cut(s) 790
BshTI ACCGGT 1 cut(s) 873
BsiHKAI GWGCWC 1 cut(s) 603
BsiSI CCGG 3 cut(s) 824, 874, 1062
BslFI GGGAC 3 cut(s) 107, 218, 703
BslI CCNNNNNNNGG 4 cut(s) 547, 548, 873, 1007
BsmAI GTCTC 2 cut(s) 372, 665
BsmBI CGTCTC 1 cut(s) 665
BsmFI GGGAC 3 cut(s) 107, 218, 703
BsnI GGCC 2 cut(s) 50, 497
Bsp1286I GDGCHC 2 cut(s) 603, 864
Bsp143I GATC 6 cut(s) 241, 612, 844, 955, 979, 1125
BspANI GGCC 2 cut(s) 50, 497
BspCNI CTCAG 5 cut(s) 271, 384, 631, 771, 1146
BspFNI CGCG 4 cut(s) 159, 181, 381, 698
BspHI TCATGA 1 cut(s) 582
BspLI GGNNCC 1 cut(s) 792
BspMAI CTGCAG 1 cut(s) 813
BspMI ACCTGC 1 cut(s) 567
BspPI GGATC 5 cut(s) 249, 607, 852, 963, 987
BspQI GCTCTTC 1 cut(s) 629
BspT107I GGYRCC 1 cut(s) 790
BsrBI CCGCTC 1 cut(s) 394
BsrDI GCAATG 1 cut(s) 1115
BsrFI RCCGGY 2 cut(s) 823, 873
BsrI ACTGG 3 cut(s) 119, 483, 691
BssAI RCCGGY 2 cut(s) 823, 873
BssECI CCNNGG 3 cut(s) 237, 526, 748
BssMI GATC 6 cut(s) 241, 612, 844, 955, 979, 1125
BssT1I CCWWGG 1 cut(s) 748
Bst4CI ACNGT 2 cut(s) 232, 1002
Bst6I CTCTTC 1 cut(s) 629
BstAPI GCANNNNNTGC 1 cut(s) 820
BstC8I GCNNGC 3 cut(s) 344, 860, 864
BstDEI CTNAG 5 cut(s) 258, 371, 618, 779, 1154
BstF5I GGATG 1 cut(s) 711
BstFNI CGCG 4 cut(s) 159, 181, 381, 698
BstH2I RGCGCY 1 cut(s) 779
BstHHI GCGC 3 cut(s) 161, 183, 778
BstKTI GATC 6 cut(s) 244, 615, 847, 958, 982, 1128
BstMAI GTCTC 2 cut(s) 372, 665
BstMBI GATC 6 cut(s) 241, 612, 844, 955, 979, 1125
BstMWI GCNNNNNNNGC 9 cut(s) 47, 408, 522, 528, 531, 641, 814, 820, 859
BstSFI CTRYAG 1 cut(s) 809
BstSLI GKGCMC 1 cut(s) 864
BstUI CGCG 4 cut(s) 159, 181, 381, 698
BstV2I GAAGAC 1 cut(s) 368
BstX2I RGATCY 3 cut(s) 241, 844, 979
BstYI RGATCY 3 cut(s) 241, 844, 979
BsuI GTATCC 1 cut(s) 873
BsuRI GGCC 2 cut(s) 50, 497
BtgZI GCGATG 1 cut(s) 256
BtsCI GGATG 1 cut(s) 711
BtsIMutI CAGTG 1 cut(s) 490
BveI ACCTGC 1 cut(s) 567
Cac8I GCNNGC 3 cut(s) 344, 860, 864
CaiI CAGNNNCTG 1 cut(s) 125
CciI TCATGA 1 cut(s) 582
CfoI GCGC 3 cut(s) 161, 183, 778
Cfr10I RCCGGY 2 cut(s) 823, 873
Cfr13I GGNCC 3 cut(s) 495, 652, 998
CpoI CGGWCCG 1 cut(s) 998
Csp6I GTAC 2 cut(s) 313, 1021
CspAI ACCGGT 1 cut(s) 873
CspI CGGWCCG 1 cut(s) 998
CviAII CATG 4 cut(s) 316, 451, 583, 742
CviQI GTAC 2 cut(s) 313, 1021
DdeI CTNAG 5 cut(s) 258, 371, 618, 779, 1154
DpnI GATC 6 cut(s) 243, 614, 846, 957, 981, 1127
DpnII GATC 6 cut(s) 241, 612, 844, 955, 979, 1125
DraIII CACNNNGTG 1 cut(s) 868
DrdI GACNNNNNNGTC 2 cut(s) 485, 592
DriI GACNNNNNGTC 1 cut(s) 15
DseDI GACNNNNNNGTC 2 cut(s) 485, 592
EaeI YGGCCR 1 cut(s) 48
Eam1104I CTCTTC 1 cut(s) 629
Eam1105I GACNNNNNGTC 1 cut(s) 15
EarI CTCTTC 1 cut(s) 629
EciI GGCGGA 1 cut(s) 696
Ecl136II GAGCTC 1 cut(s) 601
Eco130I CCWWGG 1 cut(s) 748
Eco24I GRGCYC 1 cut(s) 603
Eco47I GGWCC 2 cut(s) 652, 998
Eco53kI GAGCTC 1 cut(s) 601
Eco57I CTGAAG 1 cut(s) 502
EcoICRI GAGCTC 1 cut(s) 601
EcoT14I CCWWGG 1 cut(s) 748
EcoT38I GRGCYC 1 cut(s) 603
ErhI CCWWGG 1 cut(s) 748
Esp3I CGTCTC 1 cut(s) 665
FaeI CATG 4 cut(s) 319, 454, 586, 745
FaiI YATR 7 cut(s) 44, 59, 155, 317, 452, 584, 743
FaqI GGGAC 3 cut(s) 107, 218, 703
FatI CATG 4 cut(s) 315, 450, 582, 741
FauI CCCGC 5 cut(s) 351, 399, 407, 851, 1120
FokI GGATG 1 cut(s) 718
FriOI GRGCYC 1 cut(s) 603
GlaI GCGC 3 cut(s) 160, 182, 777
GsaI CCCAGC 1 cut(s) 118
GsuI CTGGAG 1 cut(s) 466
HaeII RGCGCY 1 cut(s) 779
HaeIII GGCC 2 cut(s) 50, 497
HapII CCGG 3 cut(s) 824, 874, 1062
HhaI GCGC 3 cut(s) 161, 183, 778
Hin1II CATG 4 cut(s) 319, 454, 586, 745
Hin6I GCGC 3 cut(s) 159, 181, 776
HinP1I GCGC 3 cut(s) 159, 181, 776
HindIII AAGCTT 1 cut(s) 514
HinfI GANTC 7 cut(s) 22, 350, 365, 454, 479, 753, 1058
HpaII CCGG 3 cut(s) 824, 874, 1062
HphI GGTGA 2 cut(s) 715, 1042
Hpy166II GTNNAC 3 cut(s) 871, 1087, 1138
Hpy188I TCNGA 5 cut(s) 21, 241, 355, 598, 998
Hpy188III TCNNGA 7 cut(s) 476, 566, 583, 610, 733, 983, 1153
Hpy8I GTNNAC 3 cut(s) 871, 1087, 1138
Hpy99I CGWCG 1 cut(s) 836
HpyAV CCTTC 5 cut(s) 340, 477, 982, 1021, 1180
HpyCH4III ACNGT 2 cut(s) 232, 1002
HpyCH4V TGCA 2 cut(s) 510, 811
HpyF10VI GCNNNNNNNGC 9 cut(s) 47, 408, 522, 528, 531, 641, 814, 820, 859
HpyF3I CTNAG 5 cut(s) 258, 371, 618, 779, 1154
Hsp92II CATG 4 cut(s) 319, 454, 586, 745
HspAI GCGC 3 cut(s) 159, 181, 776
Kzo9I GATC 6 cut(s) 241, 612, 844, 955, 979, 1125
LguI GCTCTTC 1 cut(s) 629
LmnI GCTCC 5 cut(s) 150, 399, 598, 808, 1107
LweI GCATC 2 cut(s) 675, 904
MaeIII GTNAC 3 cut(s) 7, 721, 886
MalI GATC 6 cut(s) 243, 614, 846, 957, 981, 1127
MbiI CCGCTC 1 cut(s) 394
MboI GATC 6 cut(s) 241, 612, 844, 955, 979, 1125
MboII GAAGA 5 cut(s) 368, 432, 616, 1084, 1170
MflI RGATCY 3 cut(s) 241, 844, 979
MhlI GDGCHC 2 cut(s) 603, 864
MlsI TGGCCA 1 cut(s) 50
MluCI AATT 5 cut(s) 544, 569, 795, 1013, 1142
MluNI TGGCCA 1 cut(s) 50
MlyI GAGTC 3 cut(s) 344, 374, 473
MmeI TCCRAC 4 cut(s) 378, 576, 600, 933
Mox20I TGGCCA 1 cut(s) 50
MroXI GAANNNNTTC 1 cut(s) 992
MscI TGGCCA 1 cut(s) 50
Msp20I TGGCCA 1 cut(s) 50
MspA1I CMGCKG 5 cut(s) 346, 408, 522, 808, 817
MspI CCGG 3 cut(s) 824, 874, 1062
MvnI CGCG 4 cut(s) 159, 181, 381, 698
MwoI GCNNNNNNNGC 9 cut(s) 47, 408, 522, 528, 531, 641, 814, 820, 859
NdeII GATC 6 cut(s) 241, 612, 844, 955, 979, 1125
NlaIII CATG 4 cut(s) 319, 454, 586, 745
NlaIV GGNNCC 1 cut(s) 792
NmeAIII GCCGAG 3 cut(s) 388, 507, 642
NmuCI GTSAC 2 cut(s) 7, 721
PagI TCATGA 1 cut(s) 582
PciSI GCTCTTC 1 cut(s) 629
PdmI GAANNNNTTC 1 cut(s) 992
PfeI GAWTC 4 cut(s) 22, 454, 753, 1058
PinAI ACCGGT 1 cut(s) 873
PleI GAGTC 3 cut(s) 344, 373, 473
PpsI GAGTC 3 cut(s) 344, 373, 473
Psp124BI GAGCTC 1 cut(s) 603
PspFI CCCAGC 1 cut(s) 114
PspN4I GGNNCC 1 cut(s) 792
PspPI GGNCC 3 cut(s) 495, 652, 998
PstI CTGCAG 1 cut(s) 813
PstNI CAGNNNCTG 1 cut(s) 125
PsuI RGATCY 3 cut(s) 241, 844, 979
PvuII CAGCTG 1 cut(s) 522
RsaI GTAC 2 cut(s) 314, 1022
RsaNI GTAC 2 cut(s) 313, 1021
Rsr2I CGGWCCG 1 cut(s) 998
RsrII CGGWCCG 1 cut(s) 998
SacI GAGCTC 1 cut(s) 603
SapI GCTCTTC 1 cut(s) 629
Sau3AI GATC 6 cut(s) 241, 612, 844, 955, 979, 1125
Sau96I GGNCC 3 cut(s) 495, 652, 998
SchI GAGTC 3 cut(s) 344, 374, 473
SduI GDGCHC 2 cut(s) 603, 864
SfaNI GCATC 2 cut(s) 675, 904
SfcI CTRYAG 1 cut(s) 809
SinI GGWCC 2 cut(s) 652, 998
SmlI CTYRAG 1 cut(s) 983
SmoI CTYRAG 1 cut(s) 983
Sse9I AATT 5 cut(s) 544, 569, 795, 1013, 1142
SstI GAGCTC 1 cut(s) 603
StyI CCWWGG 1 cut(s) 748
TaaI ACNGT 2 cut(s) 232, 1002
TaqI TCGA 5 cut(s) 555, 603, 669, 831, 1176
TaqII GACCGA 1 cut(s) 640
TasI AATT 5 cut(s) 544, 569, 795, 1013, 1142
TatI WGTACW 1 cut(s) 312
TauI GCSGC 6 cut(s) 384, 405, 408, 534, 681, 701
TfiI GAWTC 4 cut(s) 22, 454, 753, 1058
TscAI CASTG 1 cut(s) 490
TseFI GTSAC 2 cut(s) 7, 721
Tsp45I GTSAC 2 cut(s) 7, 721
TspDTI ATGAA 5 cut(s) 467, 571, 722, 758, 1162
TspGWI ACGGA 3 cut(s) 447, 647, 849
TspRI CASTG 1 cut(s) 490
VpaK11BI GGWCC 2 cut(s) 652, 998
XapI RAATTY 1 cut(s) 544
XmnI GAANNNNTTC 1 cut(s) 992
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.