Prupe.4G075100_v2.0.a1

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0014427
Physical Location & Seq
Reverse (-)
3675532 .. 3678405
2874 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G075100.1

Sequence Viewer

Length: 1122 bp
ATGGGTTTCACAATGGGCCTGAACCTACTGCTCTTGATGGCTATGGTGGCCACCAACATACTCTCACTCTACCATCTCTCCTCCACTATCCAAACCCCCAAATCCGCAGCTCCACAGCCAGTCCCCGACCACCTCCTCCACCAGCTCCACACCATCCGCGCCACCATCAATCACCTCACGCGCCACCACCCATCTCCACCGGCCACCACCAAAGCAGCCAAACCCACCATCCCCTCAGATCTCCTCCTCTACTCTCAGCTCTCCCCCATAGCCTCCTCCTGCCACAACCACCCTGAACTCCTCCACAAGTACATGTCCTACACCCCCTTCTCTCTCTGCCCTCTTGACTCGGACCTCGCCGAGTCACTCATTCTGCGCGGCTGCCACCCTCTTCCTCGCCGCCGCTGCTTCACCAAAACCCCCCCAAAATCGACTTCTTCTCTCCCTCAGAACCCATTTCCTTCTTCGCTTCCAGACGCCAATGTCTTGTGGACCAAGTATTCATGCAAAAGCTTCAGCTGCCTCAACCGCCAGAACCTCAATTTGGGCTTCGACCCGCCTCACGAAGTGGTCAATTTCATGACTTACAAGTCCGAGCTCGACCTCCCCATCCCTCAGCTCTTCCAAATCGCCAAGGCGGCCAACTCGGTCCTCCGGCTCGGCGTCGACATCGGCGGCGGAACCGGGTCCTTCGCAGCGAGGATGAAGCTCTACAATGTCACCATTGTCACCACGACCATGAACCTCGGGATCCCCAACAATGAGGCGGTGGCGCTGAGGGGGCTGGTGCCGATTCACGCGCCGCTGCAGCAGCGGCTGCCGGTGTTCGATGGGGTGGTGGATCTGGTGAGGTGCGGGCACGCCGTGAACCGGTGGATACCGGTGACGGCGTTGGAGTTTCTGCTGTTTGATGCGGATAGAGTGTTGAGGGGAGGAGGGTACTTGTGGCTGGACCATTTTTTCAGCAAAGAGGCGGATCTTGATAAGGTTTTTGGACCGTTGTTTGGGAAATTGGGGTACAGGAAGGTGAAGTGGGCGACGGCGAGCAAGAACGATTCGGGTGGGTTGAAGAATGGAGAGGTTTACTTGTCTGCTCTTCTCCAAAAGCCTGTCTCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_29

Protein Analysis

374

Amino Acids

41.12

Weight (kDa)

9.4

Isoelectric Point (pI)

46.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29790 AT1G29790
fragaria_vesca FvH4_3g06240 FvH4_3g06240
malus_domestica MD05G1290600.v1.1 MD16G1203100.v1.1
prunus_persica Prupe.4G075100_v2.0.a1
pyrus_communis pycom10g22250
rosa_chinensis RchiOBHm_Chr5g0013071
rosa_laevigata RLG00000031983
rosa_multiflora Rmu_sc0003892.1_g000015
rosa_rugosa Rorug05G0007000
rosa_samantha Rh5AG101000 Rh5BG097600 Rh5CG109700 Rh5DG096600
rosa_wichuraiana Rw5G008830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 482, 589
AccB1I GGYRCC 1 cut(s) 787
AccI GTMKAC 1 cut(s) 666
AccII CGCG 4 cut(s) 159, 181, 378, 800
AclWI GGATC 4 cut(s) 745, 758, 849, 984
AcoI YGGCCR 3 cut(s) 48, 201, 639
AcuI CTGAAG 1 cut(s) 499
AcyI GRCGYC 2 cut(s) 477, 663
AdeI CACNNNGTG 2 cut(s) 568, 865
AfaI GTAC 3 cut(s) 311, 941, 1019
AfiI CCNNNNNNNGG 3 cut(s) 544, 870, 1004
AflIII ACRYGT 1 cut(s) 312
AgeI ACCGGT 2 cut(s) 870, 880
AgsI TTSAA 1 cut(s) 1069
AjuI GAANNNNNNNTTGG 4 cut(s) 418, 450, 527, 559
AluBI AGCT 8 cut(s) 110, 145, 259, 513, 519, 598, 619, 709
AluI AGCT 8 cut(s) 110, 145, 259, 513, 519, 598, 619, 709
Alw21I GWGCWC 1 cut(s) 600
Alw26I GTCTC 1 cut(s) 1117
AlwI GGATC 4 cut(s) 745, 758, 849, 984
AlwNI CAGNNNCTG 1 cut(s) 817
Ama87I CYCGRG 1 cut(s) 746
AoxI GGCC 4 cut(s) 16, 48, 201, 639
AsiGI ACCGGT 2 cut(s) 870, 880
AspLEI GCGC 5 cut(s) 161, 183, 378, 775, 802
AspS9I GGNCC 7 cut(s) 16, 352, 492, 649, 687, 952, 995
AsuC2I CCSGG 1 cut(s) 685
AsuHPI GGTGA 7 cut(s) 164, 403, 712, 721, 859, 895, 1039
AvaI CYCGRG 1 cut(s) 746
AvaII GGWCC 6 cut(s) 352, 492, 649, 687, 952, 995
BaeGI GKGCMC 1 cut(s) 861
BalI TGGCCA 1 cut(s) 50
BamHI GGATCC 1 cut(s) 750
BanI GGYRCC 1 cut(s) 787
BanII GRGCYC 1 cut(s) 600
Bbv12I GWGCWC 1 cut(s) 600
BbvCI CCTCAGC 2 cut(s) 615, 776
BccI CCATC 8 cut(s) 31, 81, 161, 173, 199, 236, 617, 824
BceAI ACGGC 3 cut(s) 848, 903, 1056
BciVI GTATCC 1 cut(s) 870
BcnI CCSGG 1 cut(s) 685
BcoDI GTCTC 1 cut(s) 1117
BfmI CTRYAG 1 cut(s) 806
BfoI RGCGCY 1 cut(s) 776
BfuI GTATCC 1 cut(s) 870
BglI GCCNNNNNGGC 1 cut(s) 638
BglII AGATCT 1 cut(s) 238
Bme1390I CCNGG 1 cut(s) 685
Bme18I GGWCC 6 cut(s) 352, 492, 649, 687, 952, 995
BmeT110I CYCGRG 1 cut(s) 746
BmgT120I GGNCC 7 cut(s) 16, 352, 492, 649, 687, 952, 995
BmiI GGNNCC 4 cut(s) 682, 688, 752, 789
BmrFI CCNGG 1 cut(s) 685
BmsI GCATC 1 cut(s) 901
Bpu10I CCTNAGC 2 cut(s) 615, 776
BpuMI CCSGG 1 cut(s) 685
BsaHI GRCGYC 2 cut(s) 477, 663
BsaJI CCNNGG 2 cut(s) 633, 745
BsaWI WCCGGW 2 cut(s) 870, 880
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 3 cut(s) 544, 870, 1004
Bse118I RCCGGY 4 cut(s) 199, 820, 870, 880
Bse1I ACTGG 1 cut(s) 119
BseDI CCNNGG 2 cut(s) 633, 745
BseGI GGATG 4 cut(s) 153, 228, 609, 708
BseLI CCNNNNNNNGG 3 cut(s) 544, 870, 1004
BseMII CTCAG 5 cut(s) 249, 269, 461, 629, 767
BseNI ACTGG 1 cut(s) 119
BseRI GAGGAG 7 cut(s) 70, 125, 233, 236, 265, 290, 948
BseSI GKGCMC 1 cut(s) 861
Bsh1236I CGCG 4 cut(s) 159, 181, 378, 800
BshFI GGCC 4 cut(s) 18, 50, 203, 641
BshNI GGYRCC 1 cut(s) 787
BshTI ACCGGT 2 cut(s) 870, 880
BsiHKAI GWGCWC 1 cut(s) 600
BsiHKCI CYCGRG 1 cut(s) 746
BsiSI CCGG 6 cut(s) 200, 655, 684, 821, 871, 881
BslFI GGGAC 1 cut(s) 107
BslI CCNNNNNNNGG 3 cut(s) 544, 870, 1004
BsmAI GTCTC 1 cut(s) 1117
BsmFI GGGAC 1 cut(s) 107
BsnI GGCC 4 cut(s) 18, 50, 203, 641
BsoBI CYCGRG 1 cut(s) 746
Bsp1286I GDGCHC 2 cut(s) 600, 861
Bsp143I GATC 4 cut(s) 238, 750, 841, 976
BspANI GGCC 4 cut(s) 18, 50, 203, 641
BspCNI CTCAG 5 cut(s) 248, 268, 460, 628, 768
BspFNI CGCG 4 cut(s) 159, 181, 378, 800
BspHI TCATGA 1 cut(s) 579
BspLI GGNNCC 4 cut(s) 682, 688, 752, 789
BspMAI CTGCAG 1 cut(s) 810
BspPI GGATC 4 cut(s) 745, 758, 849, 984
BspQI GCTCTTC 2 cut(s) 626, 1101
BspT107I GGYRCC 1 cut(s) 787
BsrFI RCCGGY 4 cut(s) 199, 820, 870, 880
BsrI ACTGG 1 cut(s) 119
BssAI RCCGGY 4 cut(s) 199, 820, 870, 880
BssECI CCNNGG 2 cut(s) 633, 745
BssMI GATC 4 cut(s) 238, 750, 841, 976
BssNI GRCGYC 2 cut(s) 477, 663
BssT1I CCWWGG 1 cut(s) 633
Bst4CI ACNGT 1 cut(s) 999
Bst6I CTCTTC 3 cut(s) 396, 626, 1101
BstACI GRCGYC 2 cut(s) 477, 663
BstAPI GCANNNNNTGC 1 cut(s) 817
BstC8I GCNNGC 3 cut(s) 857, 861, 1045
BstDEI CTNAG 5 cut(s) 235, 255, 447, 615, 776
BstF5I GGATG 4 cut(s) 153, 228, 609, 708
BstFNI CGCG 4 cut(s) 159, 181, 378, 800
BstH2I RGCGCY 1 cut(s) 776
BstHHI GCGC 5 cut(s) 161, 183, 378, 775, 802
BstKTI GATC 4 cut(s) 241, 753, 844, 979
BstMAI GTCTC 1 cut(s) 1117
BstMBI GATC 4 cut(s) 238, 750, 841, 976
BstNSI RCATGY 1 cut(s) 316
BstSCI CCNGG 1 cut(s) 683
BstSFI CTRYAG 1 cut(s) 806
BstSLI GKGCMC 1 cut(s) 861
BstUI CGCG 4 cut(s) 159, 181, 378, 800
BstX2I RGATCY 4 cut(s) 238, 750, 841, 976
BstYI RGATCY 4 cut(s) 238, 750, 841, 976
BsuI GTATCC 1 cut(s) 870
BsuRI GGCC 4 cut(s) 18, 50, 203, 641
BtsCI GGATG 4 cut(s) 153, 228, 609, 708
Cac8I GCNNGC 3 cut(s) 857, 861, 1045
CaiI CAGNNNCTG 1 cut(s) 817
CciI TCATGA 1 cut(s) 579
CfoI GCGC 5 cut(s) 161, 183, 378, 775, 802
Cfr10I RCCGGY 4 cut(s) 199, 820, 870, 880
Cfr13I GGNCC 7 cut(s) 16, 352, 492, 649, 687, 952, 995
CseI GACGC 2 cut(s) 485, 652
Csp6I GTAC 3 cut(s) 310, 940, 1018
CspAI ACCGGT 2 cut(s) 870, 880
CviAII CATG 4 cut(s) 313, 504, 580, 739
CviQI GTAC 3 cut(s) 310, 940, 1018
DdeI CTNAG 5 cut(s) 235, 255, 447, 615, 776
DpnI GATC 4 cut(s) 240, 752, 843, 978
DpnII GATC 4 cut(s) 238, 750, 841, 976
DraIII CACNNNGTG 2 cut(s) 568, 865
DrdI GACNNNNNNGTC 2 cut(s) 482, 589
DseDI GACNNNNNNGTC 2 cut(s) 482, 589
EaeI YGGCCR 3 cut(s) 48, 201, 639
Eam1104I CTCTTC 3 cut(s) 396, 626, 1101
EarI CTCTTC 3 cut(s) 396, 626, 1101
EciI GGCGGA 2 cut(s) 693, 989
Ecl136II GAGCTC 1 cut(s) 598
Eco130I CCWWGG 1 cut(s) 633
Eco24I GRGCYC 1 cut(s) 600
Eco47I GGWCC 6 cut(s) 352, 492, 649, 687, 952, 995
Eco53kI GAGCTC 1 cut(s) 598
Eco57I CTGAAG 1 cut(s) 499
Eco88I CYCGRG 1 cut(s) 746
EcoICRI GAGCTC 1 cut(s) 598
EcoO109I RGGNCCY 1 cut(s) 687
EcoT14I CCWWGG 1 cut(s) 633
EcoT38I GRGCYC 1 cut(s) 600
ErhI CCWWGG 1 cut(s) 633
FaeI CATG 4 cut(s) 316, 507, 583, 742
FaiI YATR 7 cut(s) 44, 59, 269, 314, 505, 581, 740
FaqI GGGAC 1 cut(s) 107
FatI CATG 4 cut(s) 312, 503, 579, 738
FauI CCCGC 2 cut(s) 564, 848
FblI GTMKAC 1 cut(s) 666
FokI GGATG 4 cut(s) 140, 215, 596, 715
FriOI GRGCYC 1 cut(s) 600
GlaI GCGC 5 cut(s) 160, 182, 377, 774, 801
HaeII RGCGCY 1 cut(s) 776
HaeIII GGCC 4 cut(s) 18, 50, 203, 641
HapII CCGG 6 cut(s) 200, 655, 684, 821, 871, 881
HgaI GACGC 2 cut(s) 485, 652
HhaI GCGC 5 cut(s) 161, 183, 378, 775, 802
Hin1I GRCGYC 2 cut(s) 477, 663
Hin1II CATG 4 cut(s) 316, 507, 583, 742
Hin6I GCGC 5 cut(s) 159, 181, 376, 773, 800
HinP1I GCGC 5 cut(s) 159, 181, 376, 773, 800
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HindIII AAGCTT 1 cut(s) 511
HinfI GANTC 4 cut(s) 347, 362, 793, 1055
HpaII CCGG 6 cut(s) 200, 655, 684, 821, 871, 881
HphI GGTGA 7 cut(s) 164, 403, 712, 721, 859, 895, 1039
Hpy166II GTNNAC 4 cut(s) 492, 667, 868, 1084
Hpy188I TCNGA 4 cut(s) 238, 352, 450, 595
Hpy188III TCNNGA 7 cut(s) 34, 344, 473, 563, 580, 748, 980
Hpy8I GTNNAC 4 cut(s) 492, 667, 868, 1084
Hpy99I CGWCG 2 cut(s) 668, 1042
HpyAV CCTTC 4 cut(s) 337, 471, 700, 1018
HpyCH4III ACNGT 1 cut(s) 999
HpyCH4V TGCA 2 cut(s) 507, 808
HpyF3I CTNAG 5 cut(s) 235, 255, 447, 615, 776
Hsp92I GRCGYC 2 cut(s) 477, 663
Hsp92II CATG 4 cut(s) 316, 507, 583, 742
HspAI GCGC 5 cut(s) 159, 181, 376, 773, 800
Kzo9I GATC 4 cut(s) 238, 750, 841, 976
LguI GCTCTTC 2 cut(s) 626, 1101
LmnI GCTCC 2 cut(s) 115, 150
LweI GCATC 1 cut(s) 901
MaeIII GTNAC 4 cut(s) 363, 718, 727, 883
MalI GATC 4 cut(s) 240, 752, 843, 978
MboI GATC 4 cut(s) 238, 750, 841, 976
MboII GAAGA 6 cut(s) 383, 429, 456, 613, 1081, 1088
MflI RGATCY 4 cut(s) 238, 750, 841, 976
MhlI GDGCHC 2 cut(s) 600, 861
MlsI TGGCCA 1 cut(s) 50
MluCI AATT 3 cut(s) 541, 574, 1010
MluNI TGGCCA 1 cut(s) 50
MlyI GAGTC 2 cut(s) 341, 371
MmeI TCCRAC 1 cut(s) 873
Mox20I TGGCCA 1 cut(s) 50
MscI TGGCCA 1 cut(s) 50
MslI CAYNNNNRTG 1 cut(s) 737
Msp20I TGGCCA 1 cut(s) 50
MspA1I CMGCKG 4 cut(s) 405, 519, 805, 814
MspI CCGG 6 cut(s) 200, 655, 684, 821, 871, 881
MspR9I CCNGG 1 cut(s) 685
MvnI CGCG 4 cut(s) 159, 181, 378, 800
NciI CCSGG 1 cut(s) 685
NdeII GATC 4 cut(s) 238, 750, 841, 976
NlaIII CATG 4 cut(s) 316, 507, 583, 742
NlaIV GGNNCC 4 cut(s) 682, 688, 752, 789
NmeAIII GCCGAG 2 cut(s) 385, 639
NmuCI GTSAC 4 cut(s) 363, 718, 727, 883
NspI RCATGY 1 cut(s) 316
PagI TCATGA 1 cut(s) 579
PciI ACATGT 1 cut(s) 312
PciSI GCTCTTC 2 cut(s) 626, 1101
PfeI GAWTC 2 cut(s) 793, 1055
PinAI ACCGGT 2 cut(s) 870, 880
PleI GAGTC 2 cut(s) 341, 370
PpsI GAGTC 2 cut(s) 341, 370
PpuMI RGGWCCY 1 cut(s) 687
PscI ACATGT 1 cut(s) 312
Psp124BI GAGCTC 1 cut(s) 600
Psp5II RGGWCCY 1 cut(s) 687
PspN4I GGNNCC 4 cut(s) 682, 688, 752, 789
PspPI GGNCC 7 cut(s) 16, 352, 492, 649, 687, 952, 995
PspPPI RGGWCCY 1 cut(s) 687
PstI CTGCAG 1 cut(s) 810
PstNI CAGNNNCTG 1 cut(s) 817
PsuI RGATCY 4 cut(s) 238, 750, 841, 976
PvuII CAGCTG 1 cut(s) 519
RsaI GTAC 3 cut(s) 311, 941, 1019
RsaNI GTAC 3 cut(s) 310, 940, 1018
RseI CAYNNNNRTG 1 cut(s) 737
SacI GAGCTC 1 cut(s) 600
SalI GTCGAC 1 cut(s) 665
SapI GCTCTTC 2 cut(s) 626, 1101
Sau3AI GATC 4 cut(s) 238, 750, 841, 976
Sau96I GGNCC 7 cut(s) 16, 352, 492, 649, 687, 952, 995
SchI GAGTC 2 cut(s) 341, 371
ScrFI CCNGG 1 cut(s) 685
SduI GDGCHC 2 cut(s) 600, 861
SfaNI GCATC 1 cut(s) 901
SfcI CTRYAG 1 cut(s) 806
SinI GGWCC 6 cut(s) 352, 492, 649, 687, 952, 995
SmiMI CAYNNNNRTG 1 cut(s) 737
Sse9I AATT 3 cut(s) 541, 574, 1010
SstI GAGCTC 1 cut(s) 600
StyD4I CCNGG 1 cut(s) 683
StyI CCWWGG 1 cut(s) 633
TaaI ACNGT 1 cut(s) 999
TaqI TCGA 5 cut(s) 431, 552, 600, 666, 828
TaqII GACCGA 1 cut(s) 637
TasI AATT 3 cut(s) 541, 574, 1010
TatI WGTACW 1 cut(s) 309
TauI GCSGC 7 cut(s) 381, 402, 405, 641, 678, 805, 817
TfiI GAWTC 2 cut(s) 793, 1055
TseFI GTSAC 4 cut(s) 363, 718, 727, 883
Tsp45I GTSAC 4 cut(s) 363, 718, 727, 883
TspDTI ATGAA 4 cut(s) 492, 568, 719, 755
VpaK11BI GGWCC 6 cut(s) 352, 492, 649, 687, 952, 995
XceI RCATGY 1 cut(s) 316
XmiI GTMKAC 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.