Rh5AG101000

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0014427
Physical Location & Seq
Reverse (-)
9351708 .. 9355979
4272 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG101000.1

Sequence Viewer

Length: 1119 bp
ATGGGTTTCACAATGGGTCTGAACCTGCTTCTCTTAATGGCCATGGTGGCCACCAACATTCTCTCCCTCTACCACCTCTCCTCCACCATCCAATCCCCCAAACAATCCCCCCCTCCCAAGAACCCAGTCCCTGATCACCTCCTCCACCAGCTCCACACCATACGCGCCACCATCAACCACCTCACGCGCAGCCACCCACCCCCACGCGCCGCTTCCTCTTCCTCCTCCACCTTCAAACCCTCCTCGGATCTCCTCCTCTACTCCCGCCTCTCCCCAATAGCCTCCTCCTGCCACAACCACCCTGACCTCCTCAACAAGTACATGGACTACACACCCTTCTCGCTCTGCCCCACCGACTCCGACATTGCCGAGACTCTCATCCTCCGCGGCTGCCACCCCCTCCCTCGCCGCCGATGCTTCTCCAGAACGCCGCAGAAACCCACATCTTCACTTTCTCATAACCCTTTCGCTTCTTCTCTCCCGGATCAAAATGTCTTATGGGACAAGTACACCTGCAAGAGCTTCAGCTGCCTCAACCGCCACCATCCCACCGCCGGTTTCGATTTGAACGGCGAGCTGACCAATTTTATGACCTACAAGTCCGAGCTGGACCTCCCCATCCCGCAGCTCTTCCAAATCGCCAAGGCCGCCGGAGCTGTCCTCCGCCTCGGCCTCGACATCGGCGGCGGCACCGGGACCTTCGCCGCGAGAATGAAGCTGTACAATGTGACGGTTGTTACCACAACGATGAATTTTGGCGCGCCGAACAATGAGGCGGTGGCGCTGAGGGGGCTGGTGCCGCTGCACGTGCCGCTGCAGCAGCGGTTTCCGGTGTTCGATGGGGTGATGGATCTGGTGCGGTGCGGGCACGCGGTGAACAGGTGGATTCCGGTGACGGCGTTGGAGTTTTTGTTGTATGATGCGGATAGGGTGTTGAGGGGAGGAGGGTACTTGTGGTTGGATCATTTCTTCAGCAAGGGGGTGGATCTCGAGAAGGTTTTCGGGCCGTTGATTGGGAAGTTGGGGTACCGGAGAGTGAAGTGGGCGACGGCGAGTAAGAATGAGAAGAATGGGGAGGTTTACTTGACTGCTCTTCTGCAAAAGCCTGTCTCAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_29

Protein Analysis

372

Amino Acids

41.29

Weight (kDa)

9.49

Isoelectric Point (pI)

49.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29790 AT1G29790
fragaria_vesca FvH4_3g06240 FvH4_3g06240
malus_domestica MD05G1290600.v1.1 MD16G1203100.v1.1
prunus_persica Prupe.4G075100_v2.0.a1
pyrus_communis pycom10g22250
rosa_chinensis RchiOBHm_Chr5g0013071
rosa_laevigata RLG00000031983
rosa_multiflora Rmu_sc0003892.1_g000015
rosa_rugosa Rorug05G0007000
rosa_samantha Rh5AG101000 Rh5BG097600 Rh5CG109700 Rh5DG096600
rosa_wichuraiana Rw5G008830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 521
AasI GACNNNNNNGTC 1 cut(s) 598
Acc36I ACCTGC 2 cut(s) 33, 521
Acc65I GGTACC 1 cut(s) 1026
AccB1I GGYRCC 3 cut(s) 689, 796, 1026
AccII CGCG 7 cut(s) 165, 187, 207, 387, 707, 761, 872
AclWI GGATC 5 cut(s) 255, 492, 858, 969, 993
AcoI YGGCCR 2 cut(s) 39, 48
AcsI RAATTY 1 cut(s) 751
AcuI CTGAAG 2 cut(s) 508, 955
AcvI CACGTG 1 cut(s) 808
AdeI CACNNNGTG 1 cut(s) 874
AfaI GTAC 5 cut(s) 320, 509, 722, 950, 1028
AfiI CCNNNNNNNGG 2 cut(s) 554, 1013
AgsI TTSAA 2 cut(s) 235, 568
AluBI AGCT 8 cut(s) 151, 522, 528, 577, 607, 628, 656, 718
AluI AGCT 8 cut(s) 151, 522, 528, 577, 607, 628, 656, 718
Alw26I GTCTC 2 cut(s) 365, 1114
AlwI GGATC 5 cut(s) 255, 492, 858, 969, 993
AlwNI CAGNNNCTG 1 cut(s) 131
Ama87I CYCGRG 1 cut(s) 989
AoxI GGCC 5 cut(s) 39, 48, 645, 670, 1004
ApeKI GCWGC 8 cut(s) 189, 390, 528, 625, 802, 814, 817, 820
ApoI RAATTY 1 cut(s) 751
AscI GGCGCGCC 1 cut(s) 759
Asp700I GAANNNNTTC 1 cut(s) 998
Asp718I GGTACC 1 cut(s) 1026
AspLEI GCGC 6 cut(s) 167, 189, 209, 761, 763, 784
AspS9I GGNCC 3 cut(s) 610, 696, 1004
AsuC2I CCSGG 2 cut(s) 482, 694
AsuHPI GGTGA 4 cut(s) 128, 856, 886, 904
AvaI CYCGRG 1 cut(s) 989
AvaII GGWCC 2 cut(s) 610, 696
BaeGI GKGCMC 1 cut(s) 870
BalI TGGCCA 2 cut(s) 41, 50
BanI GGYRCC 3 cut(s) 689, 796, 1026
BarI GAAGNNNNNNTAC 2 cut(s) 1010, 1042
BbrPI CACGTG 1 cut(s) 808
BbvCI CCTCAGC 1 cut(s) 785
BbvI GCAGC 8 cut(s) 201, 377, 515, 637, 789, 801, 829, 832
BccI CCATC 6 cut(s) 95, 179, 552, 626, 833, 841
BceAI ACGGC 4 cut(s) 586, 912, 991, 1065
BclI TGATCA 1 cut(s) 133
BcnI CCSGG 2 cut(s) 482, 694
BcoDI GTCTC 2 cut(s) 365, 1114
BfmI CTRYAG 1 cut(s) 815
BfoI RGCGCY 1 cut(s) 785
BfuAI ACCTGC 2 cut(s) 33, 521
BglI GCCNNNNNGGC 1 cut(s) 47
Bme1390I CCNGG 2 cut(s) 482, 694
Bme18I GGWCC 2 cut(s) 610, 696
BmeT110I CYCGRG 1 cut(s) 989
BmgT120I GGNCC 3 cut(s) 610, 696, 1004
BmiI GGNNCC 4 cut(s) 691, 697, 798, 1028
BmrFI CCNGG 2 cut(s) 482, 694
BmrI ACTGGG 1 cut(s) 119
BmsI GCATC 2 cut(s) 404, 910
BmuI ACTGGG 1 cut(s) 119
BplI GAGNNNNNCTC 2 cut(s) 645, 677
BpmI CTGGAG 1 cut(s) 406
Bpu10I CCTNAGC 1 cut(s) 785
BpuEI CTTGAG 1 cut(s) 1096
BpuMI CCSGG 2 cut(s) 482, 694
BsaAI YACGTR 1 cut(s) 808
BsaJI CCNNGG 5 cut(s) 42, 243, 385, 642, 667
BsaWI WCCGGW 3 cut(s) 829, 889, 1029
BsaXI ACNNNNNCTCC 8 cut(s) 47, 62, 65, 77, 92, 95, 896, 926
Bsc4I CCNNNNNNNGG 2 cut(s) 554, 1013
Bse118I RCCGGY 1 cut(s) 554
Bse1I ACTGG 1 cut(s) 125
Bse3DI GCAATG 1 cut(s) 363
BseDI CCNNGG 5 cut(s) 42, 243, 385, 642, 667
BseGI GGATG 4 cut(s) 87, 378, 544, 618
BseLI CCNNNNNNNGG 2 cut(s) 554, 1013
BseMI GCAATG 1 cut(s) 363
BseMII CTCAG 1 cut(s) 776
BseNI ACTGG 1 cut(s) 125
BsePI GCGCGC 1 cut(s) 759
BseRI GAGGAG 9 cut(s) 70, 131, 214, 232, 242, 245, 274, 299, 957
BseSI GKGCMC 1 cut(s) 870
BseXI GCAGC 8 cut(s) 201, 377, 515, 637, 789, 801, 829, 832
BsgI GTGCAG 1 cut(s) 788
Bsh1236I CGCG 7 cut(s) 165, 187, 207, 387, 707, 761, 872
BshFI GGCC 5 cut(s) 41, 50, 647, 672, 1006
BshNI GGYRCC 3 cut(s) 689, 796, 1026
BsiHKCI CYCGRG 1 cut(s) 989
BsiSI CCGG 7 cut(s) 482, 555, 651, 693, 830, 890, 1030
BslFI GGGAC 3 cut(s) 113, 515, 709
BslI CCNNNNNNNGG 2 cut(s) 554, 1013
BsmAI GTCTC 2 cut(s) 365, 1114
BsmFI GGGAC 3 cut(s) 113, 515, 709
BsnI GGCC 5 cut(s) 41, 50, 647, 672, 1006
BsoBI CYCGRG 1 cut(s) 989
Bsp1286I GDGCHC 1 cut(s) 870
Bsp1407I TGTACA 1 cut(s) 720
Bsp143I GATC 6 cut(s) 133, 247, 484, 850, 961, 985
Bsp19I CCATGG 1 cut(s) 42
BspANI GGCC 5 cut(s) 41, 50, 647, 672, 1006
BspCNI CTCAG 1 cut(s) 777
BspFNI CGCG 7 cut(s) 165, 187, 207, 387, 707, 761, 872
BspLI GGNNCC 4 cut(s) 691, 697, 798, 1028
BspMAI CTGCAG 1 cut(s) 819
BspMI ACCTGC 2 cut(s) 33, 521
BspPI GGATC 5 cut(s) 255, 492, 858, 969, 993
BspQI GCTCTTC 2 cut(s) 635, 1098
BspT107I GGYRCC 3 cut(s) 689, 796, 1026
BsrDI GCAATG 1 cut(s) 363
BsrFI RCCGGY 1 cut(s) 554
BsrGI TGTACA 1 cut(s) 720
BsrI ACTGG 1 cut(s) 125
BssAI RCCGGY 1 cut(s) 554
BssECI CCNNGG 5 cut(s) 42, 243, 385, 642, 667
BssHII GCGCGC 1 cut(s) 759
BssMI GATC 6 cut(s) 133, 247, 484, 850, 961, 985
BssT1I CCWWGG 2 cut(s) 42, 642
Bst4CI ACNGT 1 cut(s) 733
Bst6I CTCTTC 3 cut(s) 223, 635, 1098
BstAUI TGTACA 1 cut(s) 720
BstBAI YACGTR 1 cut(s) 808
BstC8I GCNNGC 4 cut(s) 575, 761, 866, 870
BstDEI CTNAG 1 cut(s) 785
BstDSI CCRYGG 2 cut(s) 42, 385
BstF5I GGATG 4 cut(s) 87, 378, 544, 618
BstFNI CGCG 7 cut(s) 165, 187, 207, 387, 707, 761, 872
BstH2I RGCGCY 1 cut(s) 785
BstHHI GCGC 6 cut(s) 167, 189, 209, 761, 763, 784
BstKTI GATC 6 cut(s) 136, 250, 487, 853, 964, 988
BstMAI GTCTC 2 cut(s) 365, 1114
BstMBI GATC 6 cut(s) 133, 247, 484, 850, 961, 985
BstSCI CCNGG 2 cut(s) 480, 692
BstSFI CTRYAG 1 cut(s) 815
BstSLI GKGCMC 1 cut(s) 870
BstUI CGCG 7 cut(s) 165, 187, 207, 387, 707, 761, 872
BstV1I GCAGC 8 cut(s) 201, 377, 515, 637, 789, 801, 829, 832
BstX2I RGATCY 3 cut(s) 247, 850, 985
BstYI RGATCY 3 cut(s) 247, 850, 985
BsuRI GGCC 5 cut(s) 41, 50, 647, 672, 1006
BtgI CCRYGG 2 cut(s) 42, 385
BtsCI GGATG 4 cut(s) 87, 378, 544, 618
BveI ACCTGC 2 cut(s) 33, 521
Cac8I GCNNGC 4 cut(s) 575, 761, 866, 870
CaiI CAGNNNCTG 1 cut(s) 131
CfoI GCGC 6 cut(s) 167, 189, 209, 761, 763, 784
Cfr10I RCCGGY 1 cut(s) 554
Cfr13I GGNCC 3 cut(s) 610, 696, 1004
Cfr42I CCGCGG 1 cut(s) 388
Csp6I GTAC 5 cut(s) 319, 508, 721, 949, 1027
CviAII CATG 2 cut(s) 43, 322
CviQI GTAC 5 cut(s) 319, 508, 721, 949, 1027
DdeI CTNAG 1 cut(s) 785
DpnI GATC 6 cut(s) 135, 249, 486, 852, 963, 987
DpnII GATC 6 cut(s) 133, 247, 484, 850, 961, 985
DraIII CACNNNGTG 1 cut(s) 874
DrdI GACNNNNNNGTC 1 cut(s) 598
DseDI GACNNNNNNGTC 1 cut(s) 598
EaeI YGGCCR 2 cut(s) 39, 48
Eam1104I CTCTTC 3 cut(s) 223, 635, 1098
EarI CTCTTC 3 cut(s) 223, 635, 1098
EciI GGCGGA 1 cut(s) 653
Eco130I CCWWGG 2 cut(s) 42, 642
Eco47I GGWCC 2 cut(s) 610, 696
Eco57I CTGAAG 2 cut(s) 508, 955
Eco72I CACGTG 1 cut(s) 808
Eco88I CYCGRG 1 cut(s) 989
EcoO109I RGGNCCY 1 cut(s) 696
EcoT14I CCWWGG 2 cut(s) 42, 642
ErhI CCWWGG 2 cut(s) 42, 642
FaeI CATG 2 cut(s) 46, 325
FaiI YATR 7 cut(s) 44, 161, 323, 459, 499, 590, 918
FaqI GGGAC 3 cut(s) 113, 515, 709
FatI CATG 2 cut(s) 42, 321
FauI CCCGC 3 cut(s) 272, 630, 857
FbaI TGATCA 1 cut(s) 133
FokI GGATG 4 cut(s) 74, 365, 531, 605
GlaI GCGC 6 cut(s) 166, 188, 208, 760, 762, 783
GsuI CTGGAG 1 cut(s) 406
HaeII RGCGCY 1 cut(s) 785
HaeIII GGCC 5 cut(s) 41, 50, 647, 672, 1006
HapII CCGG 7 cut(s) 482, 555, 651, 693, 830, 890, 1030
HhaI GCGC 6 cut(s) 167, 189, 209, 761, 763, 784
Hin1II CATG 2 cut(s) 46, 325
Hin6I GCGC 6 cut(s) 165, 187, 207, 759, 761, 782
HinP1I GCGC 6 cut(s) 165, 187, 207, 759, 761, 782
HinfI GANTC 3 cut(s) 356, 373, 886
HpaII CCGG 7 cut(s) 482, 555, 651, 693, 830, 890, 1030
HphI GGTGA 4 cut(s) 128, 856, 886, 904
Hpy166II GTNNAC 3 cut(s) 510, 877, 1081
Hpy188I TCNGA 4 cut(s) 21, 247, 361, 604
Hpy188III TCNNGA 4 cut(s) 423, 989, 991, 1113
Hpy8I GTNNAC 3 cut(s) 510, 877, 1081
Hpy99I CGWCG 1 cut(s) 1051
HpyAV CCTTC 4 cut(s) 241, 346, 709, 988
HpyCH4III ACNGT 1 cut(s) 733
HpyCH4IV ACGT 1 cut(s) 807
HpyCH4V TGCA 4 cut(s) 516, 805, 817, 1099
HpyF3I CTNAG 1 cut(s) 785
HpySE526I ACGT 1 cut(s) 807
Hsp92II CATG 2 cut(s) 46, 325
HspAI GCGC 6 cut(s) 165, 187, 207, 759, 761, 782
KpnI GGTACC 1 cut(s) 1030
Ksp22I TGATCA 1 cut(s) 133
KspI CCGCGG 1 cut(s) 388
Kzo9I GATC 6 cut(s) 133, 247, 484, 850, 961, 985
LguI GCTCTTC 2 cut(s) 635, 1098
LmnI GCTCC 2 cut(s) 156, 653
Lsp1109I GCAGC 8 cut(s) 201, 377, 515, 637, 789, 801, 829, 832
LweI GCATC 2 cut(s) 404, 910
MaeII ACGT 1 cut(s) 807
MaeIII GTNAC 3 cut(s) 727, 736, 892
MalI GATC 6 cut(s) 135, 249, 486, 852, 963, 987
MboI GATC 6 cut(s) 133, 247, 484, 850, 961, 985
MboII GAAGA 7 cut(s) 210, 438, 465, 622, 961, 1078, 1085
MflI RGATCY 3 cut(s) 247, 850, 985
MhlI GDGCHC 1 cut(s) 870
MlsI TGGCCA 2 cut(s) 41, 50
MluCI AATT 2 cut(s) 583, 751
MluNI TGGCCA 2 cut(s) 41, 50
MlyI GAGTC 2 cut(s) 350, 367
MmeI TCCRAC 3 cut(s) 384, 882, 939
Mox20I TGGCCA 2 cut(s) 41, 50
MroXI GAANNNNTTC 1 cut(s) 998
MscI TGGCCA 2 cut(s) 41, 50
MseI TTAA 1 cut(s) 35
MslI CAYNNNNRTG 1 cut(s) 746
Msp20I TGGCCA 2 cut(s) 41, 50
MspA1I CMGCKG 5 cut(s) 387, 528, 802, 814, 823
MspI CCGG 7 cut(s) 482, 555, 651, 693, 830, 890, 1030
MspR9I CCNGG 2 cut(s) 482, 694
MvnI CGCG 7 cut(s) 165, 187, 207, 387, 707, 761, 872
NciI CCSGG 2 cut(s) 482, 694
NcoI CCATGG 1 cut(s) 42
NdeII GATC 6 cut(s) 133, 247, 484, 850, 961, 985
NlaIII CATG 2 cut(s) 46, 325
NlaIV GGNNCC 4 cut(s) 691, 697, 798, 1028
NmeAIII GCCGAG 2 cut(s) 394, 648
NmuCI GTSAC 2 cut(s) 727, 892
PaeR7I CTCGAG 1 cut(s) 989
PalAI GGCGCGCC 1 cut(s) 759
PaqCI CACCTGC 1 cut(s) 521
PauI GCGCGC 1 cut(s) 759
PciSI GCTCTTC 2 cut(s) 635, 1098
PdmI GAANNNNTTC 1 cut(s) 998
PfeI GAWTC 1 cut(s) 886
PfoI TCCNGGA 1 cut(s) 480
PleI GAGTC 2 cut(s) 350, 367
PmaCI CACGTG 1 cut(s) 808
PmlI CACGTG 1 cut(s) 808
PpsI GAGTC 2 cut(s) 350, 367
Ppu21I YACGTR 1 cut(s) 808
PpuMI RGGWCCY 1 cut(s) 696
Psp5II RGGWCCY 1 cut(s) 696
PspCI CACGTG 1 cut(s) 808
PspN4I GGNNCC 4 cut(s) 691, 697, 798, 1028
PspPI GGNCC 3 cut(s) 610, 696, 1004
PspPPI RGGWCCY 1 cut(s) 696
PstI CTGCAG 1 cut(s) 819
PstNI CAGNNNCTG 1 cut(s) 131
PsuI RGATCY 3 cut(s) 247, 850, 985
PteI GCGCGC 1 cut(s) 759
PvuII CAGCTG 1 cut(s) 528
RsaI GTAC 5 cut(s) 320, 509, 722, 950, 1028
RsaNI GTAC 5 cut(s) 319, 508, 721, 949, 1027
RseI CAYNNNNRTG 1 cut(s) 746
SacII CCGCGG 1 cut(s) 388
SapI GCTCTTC 2 cut(s) 635, 1098
SaqAI TTAA 1 cut(s) 35
Sau3AI GATC 6 cut(s) 133, 247, 484, 850, 961, 985
Sau96I GGNCC 3 cut(s) 610, 696, 1004
SchI GAGTC 2 cut(s) 350, 367
ScrFI CCNGG 2 cut(s) 482, 694
SduI GDGCHC 1 cut(s) 870
SfaNI GCATC 2 cut(s) 404, 910
SfcI CTRYAG 1 cut(s) 815
SfiI GGCCNNNNNGGCC 1 cut(s) 47
Sfr274I CTCGAG 1 cut(s) 989
Sfr303I CCGCGG 1 cut(s) 388
SgrBI CCGCGG 1 cut(s) 388
SgsI GGCGCGCC 1 cut(s) 759
SinI GGWCC 2 cut(s) 610, 696
SlaI CTCGAG 1 cut(s) 989
SmiMI CAYNNNNRTG 1 cut(s) 746
SmlI CTYRAG 2 cut(s) 989, 1111
SmoI CTYRAG 2 cut(s) 989, 1111
Sse9I AATT 2 cut(s) 583, 751
StyD4I CCNGG 2 cut(s) 480, 692
StyI CCWWGG 2 cut(s) 42, 642
TaaI ACNGT 1 cut(s) 733
TaiI ACGT 1 cut(s) 810
TaqI TCGA 4 cut(s) 561, 675, 837, 990
TasI AATT 2 cut(s) 583, 751
TatI WGTACW 3 cut(s) 318, 507, 720
TfiI GAWTC 1 cut(s) 886
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TseFI GTSAC 2 cut(s) 727, 892
TseI GCWGC 8 cut(s) 189, 390, 528, 625, 802, 814, 817, 820
Tsp45I GTSAC 2 cut(s) 727, 892
TspDTI ATGAA 2 cut(s) 728, 764
VpaK11BI GGWCC 2 cut(s) 610, 696
XapI RAATTY 1 cut(s) 751
XhoI CTCGAG 1 cut(s) 989
XmnI GAANNNNTTC 1 cut(s) 998
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.