MD05G1298500.v1.1

nucleobase-ascorbate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008195
Physical Location & Seq
Reverse (-)
43191562 .. 43195858
4297 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1298500.v1.1.491

Sequence Viewer

Length: 1575 bp
ATGGCAGCTCCTAAACCAGAAGAGATAAGCCATCCAGCAATGGACCAACTTCAAGGCTTAGAGTACTGCATTGACTCAAATCCCTCTTGGGGGGAAGCAATTGCTTTGGGGTTCCAGCACTACATTTTGGCTCTAGGAACTGCTGTGATGATCCCATCATTCCTTGTTCCTTTGATGGGTGGTGATGATGGTGACAAAGTGAGAGTAGTGCAGACTCTACTTTTTATTCAAGGAATCAACACCCTTCTGCAAACATTATTTGGAACCCGATTGCCAACCGTAATTGGAGGGTCTTACGCATTCATGGTCCCGATAATATCCATAATTCATGATTCACGGTTGATGAAGATTGAGGACAATCATGTGAGATTCCTTGACACCATGAAAGCAGTCCAAGGTGCTCTGATAGTAGCTTCAAGCATACAAATTGTTCTGGGATATAGTCAAATTTGGGCCATCTGTTCCAGGTTCTTCAGCCCGCTTGGAATGGTCCCGGTTATTTCCTTGGTGGGTTTTGGCCTGTTTGATAGAGGCTTCCCCGTGGTTGGAAAGTGTGTGGAAATTGGCATTCCCATGCTTATCCTTTTCGTAGCTTTCTCTCAGTACCTAAAGAACTTCCATGCAAGACAAGTACCAGTTTTAGAACGATTTGCTCTTCTTATATCAGTCACAGTGATATGGGCATACGCACACCTCTTGACCGCCAGTGGCGCATACAGACACCGCCCAGACATAACCCAAGTCAATTGCCGAACTGACAGGGCCAACCTCATTTCTTCTGCTCCATGGATAAAAGTACCTTATCCTCTTCAATGGGGTGCCCCAACCTTTGATGCTGGTCATGCTTTTGGAATGATGGCAGCTGTTCTTGTTTCATTGATTGAGTCAACTGGAGCATACAAGGCTGCATCGCGTCTAGCAAGTGCCACAATACCTCCAGCTCATGTTCTTAGCCGTGGAATTGGCTGGCAGGGGATAGGAATCCTACTTAATGGACTCTTTGGAACGTTGACTGGCTCAACAGTCTCTATAGAAAATGTGGGGCTTCTTGGGAGCACTCGTGTTGGAAGCCGTAGGGTCATTCAAATCTCAGCCGGTTTTATGATATTCTTCTCCATGTTAGGTAAATTTGGAGCTCTGTTTGCATCAATACCCTTCACCATATTTGCTGCTGCCTATTGTGTATTGTTTGGTCTTGTCGCTTCGGTGGGGTTATCCTTTTTGCAATTCACAAACATGAACTCGATGAGGAACCTCTTTATTGTTGGTGTAGCGTTATTCTTGGGTTTGTCTGTTCCTGAGTACTTCAGAGGTTACACTTTGAAGGCTTTGCACGGTCCTGCTCATACCAAAGCAGGATGGTTCAACGATTTTCTGAATACCATCTTCTCCTCATCTCCGACTGTGGCCTTGTTTGTTGCTGTTTTCCTGGATAACACACTTGAGTACAAGGACAGTGCAAGAGACAGAGGAATGCCATGGTGGGTCAAATTTCGGCAATTCAAAGGGGACAGCCGCAACGAGGAGTTTTACACTCTCCCTTTCAACCTCAACCGATTCTTCCCTCCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Xan_ur_permease

Protein Analysis

525

Amino Acids

57.46

Weight (kDa)

9.21

Isoelectric Point (pI)

31.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Xan_ur_permease PF00860 32 - 436 1.1e-62 Permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 818
AccII CGCG 1 cut(s) 913
AciI CCGC 4 cut(s) 479, 702, 724, 1516
AclI AACGTT 1 cut(s) 1007
AclWI GGATC 1 cut(s) 145
AcsI RAATTY 3 cut(s) 447, 1127, 1490
AcuI CTGAAG 2 cut(s) 457, 1291
AfaI GTAC 6 cut(s) 65, 605, 633, 798, 1304, 1448
AfiI CCNNNNNNNGG 5 cut(s) 89, 90, 176, 545, 1522
AgsI TTSAA 9 cut(s) 53, 230, 417, 812, 1085, 1324, 1366, 1504, 1546
AjnI CCWGG 2 cut(s) 464, 1428
AluBI AGCT 6 cut(s) 8, 413, 593, 863, 941, 1136
AluI AGCT 6 cut(s) 8, 413, 593, 863, 941, 1136
Alw21I GWGCWC 3 cut(s) 403, 1058, 1138
Alw26I GTCTC 2 cut(s) 1030, 1458
AlwI GGATC 1 cut(s) 145
AoxI GGCC 4 cut(s) 453, 517, 762, 1407
ApeKI GCWGC 5 cut(s) 5, 860, 905, 1169, 1172
ApoI RAATTY 3 cut(s) 447, 1127, 1490
AspLEI GCGC 1 cut(s) 713
AspS9I GGNCC 6 cut(s) 43, 307, 453, 490, 762, 1337
AsuC2I CCSGG 1 cut(s) 494
AsuHPI GGTGA 3 cut(s) 194, 203, 1150
AvaII GGWCC 4 cut(s) 43, 307, 490, 1337
BaeGI GKGCMC 1 cut(s) 823
BanI GGYRCC 1 cut(s) 818
BanII GRGCYC 1 cut(s) 1138
BauI CACGAG 1 cut(s) 1059
Bbv12I GWGCWC 3 cut(s) 403, 1058, 1138
BbvI GCAGC 5 cut(s) 17, 872, 892, 1156, 1159
BccI CCATC 8 cut(s) 39, 163, 169, 182, 464, 850, 1353, 1391
BceAI ACGGC 2 cut(s) 939, 1056
BciT130I CCWGG 2 cut(s) 466, 1430
BcnI CCSGG 1 cut(s) 494
BcoDI GTCTC 2 cut(s) 1030, 1458
BfaI CTAG 2 cut(s) 134, 917
BfmI CTRYAG 1 cut(s) 1029
BisI GCNGC 6 cut(s) 6, 861, 906, 1170, 1173, 1516
BlsI GCNGC 6 cut(s) 7, 862, 907, 1171, 1174, 1517
BmcAI AGTACT 2 cut(s) 65, 1304
Bme1390I CCNGG 3 cut(s) 466, 494, 1430
Bme18I GGWCC 4 cut(s) 43, 307, 490, 1337
BmgT120I GGNCC 6 cut(s) 43, 307, 453, 490, 762, 1337
BmiI GGNNCC 6 cut(s) 113, 265, 309, 492, 820, 1253
BmrFI CCNGG 3 cut(s) 466, 494, 1430
BmsI GCATC 3 cut(s) 823, 917, 1154
BpmI CTGGAG 2 cut(s) 912, 921
BpuEI CTTGAG 1 cut(s) 1463
BpuMI CCSGG 1 cut(s) 494
BsaBI GATNNNNATC 1 cut(s) 980
BsaJI CCNNGG 6 cut(s) 394, 504, 540, 785, 955, 1478
BsaXI ACNNNNNCTCC 4 cut(s) 919, 949, 1045, 1075
Bsc4I CCNNNNNNNGG 5 cut(s) 89, 90, 176, 545, 1522
Bse118I RCCGGY 1 cut(s) 1094
Bse1I ACTGG 4 cut(s) 635, 705, 895, 1018
Bse3DI GCAATG 1 cut(s) 45
Bse8I GATNNNNATC 1 cut(s) 980
BseBI CCWGG 2 cut(s) 466, 1430
BseDI CCNNGG 6 cut(s) 394, 504, 540, 785, 955, 1478
BseGI GGATG 2 cut(s) 31, 1364
BseJI GATNNNNATC 1 cut(s) 980
BseLI CCNNNNNNNGG 5 cut(s) 89, 90, 176, 545, 1522
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 3 cut(s) 614, 1104, 1290
BseNI ACTGG 4 cut(s) 635, 705, 895, 1018
BseRI GAGGAG 2 cut(s) 1381, 1538
BseSI GKGCMC 1 cut(s) 823
BseXI GCAGC 5 cut(s) 17, 872, 892, 1156, 1159
BsgI GTGCAG 1 cut(s) 230
Bsh1236I CGCG 1 cut(s) 913
BshFI GGCC 4 cut(s) 455, 519, 764, 1409
BshNI GGYRCC 1 cut(s) 818
BsiHKAI GWGCWC 3 cut(s) 403, 1058, 1138
BsiSI CCGG 2 cut(s) 494, 1095
BslFI GGGAC 3 cut(s) 293, 476, 1523
BslI CCNNNNNNNGG 5 cut(s) 89, 90, 176, 545, 1522
BsmAI GTCTC 2 cut(s) 1030, 1458
BsmFI GGGAC 3 cut(s) 293, 476, 1523
BsmI GAATGC 3 cut(s) 299, 567, 1479
BsnI GGCC 4 cut(s) 455, 519, 764, 1409
Bsp1286I GDGCHC 4 cut(s) 403, 823, 1058, 1138
Bsp143I GATC 1 cut(s) 150
Bsp19I CCATGG 2 cut(s) 785, 1478
BspACI CCGC 4 cut(s) 479, 702, 724, 1516
BspANI GGCC 4 cut(s) 455, 519, 764, 1409
BspCNI CTCAG 3 cut(s) 613, 1103, 1291
BspFNI CGCG 1 cut(s) 913
BspHI TCATGA 1 cut(s) 328
BspLI GGNNCC 6 cut(s) 113, 265, 309, 492, 820, 1253
BspPI GGATC 1 cut(s) 145
BspQI GCTCTTC 1 cut(s) 660
BspT107I GGYRCC 1 cut(s) 818
BsrDI GCAATG 1 cut(s) 45
BsrFI RCCGGY 1 cut(s) 1094
BsrI ACTGG 4 cut(s) 635, 705, 895, 1018
BssAI RCCGGY 1 cut(s) 1094
BssECI CCNNGG 6 cut(s) 394, 504, 540, 785, 955, 1478
BssMI GATC 1 cut(s) 150
BssSI CACGAG 1 cut(s) 1059
BssT1I CCWWGG 4 cut(s) 394, 504, 785, 1478
Bst2BI CACGAG 1 cut(s) 1059
Bst2UI CCWGG 2 cut(s) 466, 1430
Bst4CI ACNGT 7 cut(s) 280, 339, 673, 1024, 1337, 1405, 1457
Bst6I CTCTTC 3 cut(s) 15, 660, 813
BstC8I GCNNGC 2 cut(s) 479, 968
BstDEI CTNAG 5 cut(s) 58, 600, 950, 1090, 1299
BstDSI CCRYGG 4 cut(s) 540, 785, 955, 1478
BstF5I GGATG 2 cut(s) 31, 1364
BstFNI CGCG 1 cut(s) 913
BstHHI GCGC 1 cut(s) 713
BstKTI GATC 1 cut(s) 153
BstMAI GTCTC 2 cut(s) 1030, 1458
BstMBI GATC 1 cut(s) 150
BstMWI GCNNNNNNNGC 4 cut(s) 710, 842, 902, 1142
BstNI CCWGG 2 cut(s) 466, 1430
BstSCI CCNGG 3 cut(s) 464, 492, 1428
BstSFI CTRYAG 1 cut(s) 1029
BstSLI GKGCMC 1 cut(s) 823
BstUI CGCG 1 cut(s) 913
BstV1I GCAGC 5 cut(s) 17, 872, 892, 1156, 1159
BsuRI GGCC 4 cut(s) 455, 519, 764, 1409
BtgI CCRYGG 4 cut(s) 540, 785, 955, 1478
BtgZI GCGATG 1 cut(s) 894
BtsCI GGATG 2 cut(s) 31, 1364
BtsIMutI CAGTG 3 cut(s) 678, 712, 1462
Cac8I GCNNGC 2 cut(s) 479, 968
CciI TCATGA 1 cut(s) 328
CfoI GCGC 1 cut(s) 713
Cfr10I RCCGGY 1 cut(s) 1094
Cfr13I GGNCC 6 cut(s) 43, 307, 453, 490, 762, 1337
CseI GACGC 1 cut(s) 902
Csp6I GTAC 6 cut(s) 64, 604, 632, 797, 1303, 1447
CviQI GTAC 6 cut(s) 64, 604, 632, 797, 1303, 1447
DdeI CTNAG 5 cut(s) 58, 600, 950, 1090, 1299
DpnI GATC 1 cut(s) 152
DpnII GATC 1 cut(s) 150
Eam1104I CTCTTC 3 cut(s) 15, 660, 813
EarI CTCTTC 3 cut(s) 15, 660, 813
Ecl136II GAGCTC 1 cut(s) 1136
Eco130I CCWWGG 4 cut(s) 394, 504, 785, 1478
Eco24I GRGCYC 1 cut(s) 1138
Eco47I GGWCC 4 cut(s) 43, 307, 490, 1337
Eco53kI GAGCTC 1 cut(s) 1136
Eco57I CTGAAG 2 cut(s) 457, 1291
EcoICRI GAGCTC 1 cut(s) 1136
EcoRII CCWGG 2 cut(s) 464, 1428
EcoT14I CCWWGG 4 cut(s) 394, 504, 785, 1478
EcoT38I GRGCYC 1 cut(s) 1138
ErhI CCWWGG 4 cut(s) 394, 504, 785, 1478
FaqI GGGAC 3 cut(s) 293, 476, 1523
FauI CCCGC 1 cut(s) 486
Fnu4HI GCNGC 6 cut(s) 6, 861, 906, 1170, 1173, 1516
FokI GGATG 2 cut(s) 18, 1371
FriOI GRGCYC 1 cut(s) 1138
Fsp4HI GCNGC 6 cut(s) 6, 861, 906, 1170, 1173, 1516
FspBI CTAG 2 cut(s) 134, 917
GlaI GCGC 1 cut(s) 712
GluI GCNGC 6 cut(s) 6, 861, 906, 1170, 1173, 1516
GsuI CTGGAG 2 cut(s) 912, 921
HaeIII GGCC 4 cut(s) 455, 519, 764, 1409
HapII CCGG 2 cut(s) 494, 1095
HgaI GACGC 1 cut(s) 902
HhaI GCGC 1 cut(s) 713
Hin6I GCGC 1 cut(s) 711
HinP1I GCGC 1 cut(s) 711
HincII GTYRAC 2 cut(s) 888, 1011
HindII GTYRAC 2 cut(s) 888, 1011
HinfI GANTC 9 cut(s) 74, 214, 234, 332, 369, 884, 981, 996, 1557
HpaII CCGG 2 cut(s) 494, 1095
HphI GGTGA 3 cut(s) 194, 203, 1150
Hpy166II GTNNAC 2 cut(s) 888, 1011
Hpy188I TCNGA 4 cut(s) 405, 1310, 1377, 1401
Hpy188III TCNNGA 5 cut(s) 310, 329, 697, 1298, 1572
Hpy8I GTNNAC 2 cut(s) 888, 1011
HpyAV CCTTC 3 cut(s) 254, 1165, 1318
HpyCH4III ACNGT 7 cut(s) 280, 339, 673, 1024, 1337, 1405, 1457
HpyCH4IV ACGT 1 cut(s) 1007
HpyCH4V TGCA 9 cut(s) 69, 211, 250, 623, 908, 1145, 1225, 1333, 1460
HpyF10VI GCNNNNNNNGC 4 cut(s) 710, 842, 902, 1142
HpyF3I CTNAG 5 cut(s) 58, 600, 950, 1090, 1299
HpySE526I ACGT 1 cut(s) 1007
HspAI GCGC 1 cut(s) 711
Kzo9I GATC 1 cut(s) 150
LguI GCTCTTC 1 cut(s) 660
LmnI GCTCC 5 cut(s) 13, 787, 893, 1053, 1133
Lsp1109I GCAGC 5 cut(s) 17, 872, 892, 1156, 1159
LweI GCATC 3 cut(s) 823, 917, 1154
MaeI CTAG 2 cut(s) 134, 917
MaeII ACGT 1 cut(s) 1007
MaeIII GTNAC 3 cut(s) 191, 667, 1313
MalI GATC 1 cut(s) 152
MboI GATC 1 cut(s) 150
MboII GAAGA 9 cut(s) 32, 358, 463, 647, 768, 800, 1102, 1378, 1552
MfeI CAATTG 2 cut(s) 99, 745
MhlI GDGCHC 4 cut(s) 403, 823, 1058, 1138
MlyI GAGTC 4 cut(s) 68, 208, 893, 990
MmeI TCCRAC 3 cut(s) 526, 1045, 1424
MseI TTAA 1 cut(s) 990
MslI CAYNNNNRTG 2 cut(s) 572, 1235
MspA1I CMGCKG 1 cut(s) 863
MspI CCGG 2 cut(s) 494, 1095
MspR9I CCNGG 3 cut(s) 466, 494, 1430
MunI CAATTG 2 cut(s) 99, 745
Mva1269I GAATGC 3 cut(s) 299, 567, 1479
MvaI CCWGG 2 cut(s) 466, 1430
MvnI CGCG 1 cut(s) 913
MwoI GCNNNNNNNGC 4 cut(s) 710, 842, 902, 1142
NciI CCSGG 1 cut(s) 494
NcoI CCATGG 2 cut(s) 785, 1478
NdeII GATC 1 cut(s) 150
NlaIV GGNNCC 6 cut(s) 113, 265, 309, 492, 820, 1253
NmuCI GTSAC 2 cut(s) 191, 667
PagI TCATGA 1 cut(s) 328
PciSI GCTCTTC 1 cut(s) 660
PctI GAATGC 3 cut(s) 299, 567, 1479
PfeI GAWTC 5 cut(s) 234, 332, 369, 981, 1557
PfoI TCCNGGA 1 cut(s) 1428
PkrI GCNGC 6 cut(s) 7, 862, 907, 1171, 1174, 1517
PleI GAGTC 4 cut(s) 68, 208, 892, 990
PpsI GAGTC 4 cut(s) 68, 208, 892, 990
Psp124BI GAGCTC 1 cut(s) 1138
Psp1406I AACGTT 1 cut(s) 1007
Psp6I CCWGG 2 cut(s) 464, 1428
PspGI CCWGG 2 cut(s) 464, 1428
PspN4I GGNNCC 6 cut(s) 113, 265, 309, 492, 820, 1253
PspPI GGNCC 6 cut(s) 43, 307, 453, 490, 762, 1337
PvuII CAGCTG 1 cut(s) 863
RsaI GTAC 6 cut(s) 65, 605, 633, 798, 1304, 1448
RsaNI GTAC 6 cut(s) 64, 604, 632, 797, 1303, 1447
RseI CAYNNNNRTG 2 cut(s) 572, 1235
SacI GAGCTC 1 cut(s) 1138
SapI GCTCTTC 1 cut(s) 660
SaqAI TTAA 1 cut(s) 990
SatI GCNGC 6 cut(s) 6, 861, 906, 1170, 1173, 1516
Sau3AI GATC 1 cut(s) 150
Sau96I GGNCC 6 cut(s) 43, 307, 453, 490, 762, 1337
ScaI AGTACT 2 cut(s) 65, 1304
SchI GAGTC 4 cut(s) 68, 208, 893, 990
ScrFI CCNGG 3 cut(s) 466, 494, 1430
SduI GDGCHC 4 cut(s) 403, 823, 1058, 1138
SfaNI GCATC 3 cut(s) 823, 917, 1154
SfcI CTRYAG 1 cut(s) 1029
SinI GGWCC 4 cut(s) 43, 307, 490, 1337
SmiMI CAYNNNNRTG 2 cut(s) 572, 1235
SmlI CTYRAG 1 cut(s) 1442
SmoI CTYRAG 1 cut(s) 1442
SsiI CCGC 4 cut(s) 479, 702, 724, 1516
SspMI CTAG 2 cut(s) 134, 917
SstI GAGCTC 1 cut(s) 1138
StyD4I CCNGG 3 cut(s) 464, 492, 1428
StyI CCWWGG 4 cut(s) 394, 504, 785, 1478
TaaI ACNGT 7 cut(s) 280, 339, 673, 1024, 1337, 1405, 1457
TaiI ACGT 1 cut(s) 1010
TaqI TCGA 1 cut(s) 1244
TatI WGTACW 3 cut(s) 63, 1302, 1446
TauI GCSGC 1 cut(s) 1518
TfiI GAWTC 5 cut(s) 234, 332, 369, 981, 1557
Tru1I TTAA 1 cut(s) 990
Tru9I TTAA 1 cut(s) 990
TscAI CASTG 3 cut(s) 678, 712, 1462
TseFI GTSAC 2 cut(s) 191, 667
TseI GCWGC 5 cut(s) 5, 860, 905, 1169, 1172
Tsp45I GTSAC 2 cut(s) 191, 667
TspDTI ATGAA 6 cut(s) 292, 317, 359, 398, 864, 1253
TspRI CASTG 3 cut(s) 678, 712, 1462
VpaK11BI GGWCC 4 cut(s) 43, 307, 490, 1337
XapI RAATTY 3 cut(s) 447, 1127, 1490
XspI CTAG 2 cut(s) 134, 917
ZrmI AGTACT 2 cut(s) 65, 1304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.