MD10G1277800.v1.1

nucleobase-ascorbate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008195
Physical Location & Seq
Reverse (-)
36842158 .. 36845866
3709 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1277800.v1.1.491

Sequence Viewer

Length: 1578 bp
ATGGAAGCTCCAAAACCAGAAGAGATAAGCAGCCATCCAGCAATGGACCAACTCCAAGGCTTAGAGTACTGCATTGACTCAAATCCCTCTTGGGCGGAAGCAATTGCTTTGGGGTTCCAGCACTACATTTTGGCCTTGGGAACTGCTGTGATGATCCCCACATTCCTTGTTCCTTTCATGGGTGGTGATGATGGTGACAAAGTGAGAGTAGTGCAGACTCTCCTTTTTATTCAAGGAATCAACACACTTCTACAAACATTGTTTGGAACCCGATTGCCAACCGTGATTGGAGGGTCTTACGCATTCATGGTCCCGATAATATCCATAATTCATGATTCACGCTTGATGAAGGTTGAGGACAATCATGCGAGATTCCTTGACACCATGAGAGCAGTCCAAGGGGCTCTGATAGTAGCTTCAAGCATACAAATTATTCTGGGATATAGTCAAATTTGGGCCATTTGTTCCAGGTTCTTCAGCCCGCTCGGAATGGTTCCAGTTATTTCCTTGGTGGGTTTTGGTCTGTTTGATAGAGGCTTCCCCCTGGTTGGAAAGTGTGTGGAAATTGGCATTCCCATGCTTATCCTTTTTGTAGCTTTCTCTCAGTACCTAAAGAACTTCCATGCAAGACAGCTACCAGTATTAGAACGATTTGCTCTTCTTATATCAATCACAGTGATATGGGCATATGCACACCTCTTGACCGCCAGTGGTGCATACAAACACCGGCCAGACATAACCCAAGTCAATTGCCGAACTGATAGGGCCAACCTCATTTCTTCTGCTCCATGGATAAAAATACCTTATCCTCTTCAATGGGGTGCCCCAACCTTTGATGTTGGTCATGCTTTTGGAATGATGGCTGCCGTTCTTGTTTCATTGATCGAGTCAACTGGAGCATACAAGGCTGCATCGCGTCTAGCAAGTGCCACAATACCTCCAGCTCATGTTCTCAGCCGTGGAATTGGCTGGCAGGGAATAGGAATCCTACTAAATGGACTCTTTGGGACGTTGTCAGGCTCAACAGTCTCTATAGAAAATGTGGGGCTTCTTGGGAGCACTCGTGTTGGAAGCCGTAGGGTCATTCAAATCTCGGCCGGTTTTATGATATTCTTCTCCATGTTAGGTAAATTTGGTGCTCTGTTCGCATCAATACCCTTCACCTTATTTGCTGCTGCATATTGTGTATTGTTTGGTCTTGTTGCTTCGGTGGGGTTATCCTTTTTGCAATTCACAAACATGAACTCGACGAGGAACCTCTTTATTGTTGGTGTAGCTTTCTTCTTGGGTTTGTCTGTTCCCGAGTACTTCAGGGGTTACACTTTGAAGGCTTTCCATGGTCCTGCTCATACCAATGCCGGATGGTTCAACGATTTTCTGAATACCATCTTCTCCTCATCTCCAACTGTGGCATTGATTGTTGCCGTTTTCCTGGATAACACACTTGAGTACAAGGACAGTGCAAGAGACAGAGGAATGCCGTGGTGGGTCAAATTTCGGCAATTCAAAGGGGACAGCCGCAACGAGGAGTTTTACACTCTCCCCTTCAACCTTAACCGGTTCTTCCCTCCATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Xan_ur_permease

Protein Analysis

526

Amino Acids

57.7

Weight (kDa)

9.01

Isoelectric Point (pI)

30.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Xan_ur_permease PF00860 34 - 437 1.5e-62 Permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 821
AccBSI CCGCTC 1 cut(s) 484
AccII CGCG 1 cut(s) 916
AciI CCGC 4 cut(s) 95, 482, 705, 1519
AclWI GGATC 1 cut(s) 148
AcoI YGGCCR 2 cut(s) 728, 1095
AcsI RAATTY 3 cut(s) 450, 1130, 1493
AcuI CTGAAG 2 cut(s) 460, 1294
AfaI GTAC 4 cut(s) 68, 608, 1307, 1451
AfiI CCNNNNNNNGG 3 cut(s) 179, 548, 1525
AgeI ACCGGT 1 cut(s) 1557
AgsI TTSAA 8 cut(s) 233, 420, 815, 1088, 1327, 1369, 1507, 1549
AjnI CCWGG 3 cut(s) 467, 543, 1431
AluBI AGCT 6 cut(s) 8, 416, 596, 634, 944, 1277
AluI AGCT 6 cut(s) 8, 416, 596, 634, 944, 1277
Alw21I GWGCWC 2 cut(s) 1061, 1141
Alw26I GTCTC 2 cut(s) 1033, 1461
AlwI GGATC 1 cut(s) 148
Ama87I CYCGRG 1 cut(s) 1301
AoxI GGCC 5 cut(s) 132, 456, 728, 765, 1095
ApeKI GCWGC 5 cut(s) 30, 863, 908, 1172, 1175
ApoI RAATTY 3 cut(s) 450, 1130, 1493
AsiGI ACCGGT 1 cut(s) 1557
Asp700I GAANNNNTTC 1 cut(s) 1331
AspS9I GGNCC 5 cut(s) 46, 310, 456, 765, 1340
AsuHPI GGTGA 3 cut(s) 197, 206, 1153
AvaI CYCGRG 1 cut(s) 1301
AvaII GGWCC 3 cut(s) 46, 310, 1340
BaeGI GKGCMC 1 cut(s) 826
BanI GGYRCC 1 cut(s) 821
BanII GRGCYC 1 cut(s) 406
BauI CACGAG 1 cut(s) 1062
Bbv12I GWGCWC 2 cut(s) 1061, 1141
BbvI GCAGC 5 cut(s) 42, 850, 895, 1159, 1162
BccI CCATC 5 cut(s) 42, 185, 853, 1356, 1394
BceAI ACGGC 5 cut(s) 851, 942, 1059, 1409, 1465
BciT130I CCWGG 3 cut(s) 469, 545, 1433
BcoDI GTCTC 2 cut(s) 1033, 1461
BfaI CTAG 1 cut(s) 920
BfmI CTRYAG 1 cut(s) 1032
BisI GCNGC 6 cut(s) 31, 864, 909, 1173, 1176, 1519
BlsI GCNGC 6 cut(s) 32, 865, 910, 1174, 1177, 1520
BmcAI AGTACT 2 cut(s) 68, 1307
Bme1390I CCNGG 3 cut(s) 469, 545, 1433
Bme18I GGWCC 3 cut(s) 46, 310, 1340
BmeT110I CYCGRG 1 cut(s) 1301
BmgT120I GGNCC 5 cut(s) 46, 310, 456, 765, 1340
BmiI GGNNCC 6 cut(s) 116, 268, 312, 495, 823, 1256
BmrFI CCNGG 3 cut(s) 469, 545, 1433
BmsI GCATC 2 cut(s) 920, 1157
BpmI CTGGAG 2 cut(s) 915, 924
BpuEI CTTGAG 1 cut(s) 1466
BsaJI CCNNGG 9 cut(s) 55, 135, 397, 507, 543, 788, 958, 1336, 1481
BsaWI WCCGGW 1 cut(s) 1557
BsaXI ACNNNNNCTCC 4 cut(s) 922, 952, 1048, 1078
Bsc4I CCNNNNNNNGG 3 cut(s) 179, 548, 1525
Bse118I RCCGGY 3 cut(s) 726, 1097, 1557
Bse1I ACTGG 4 cut(s) 497, 638, 708, 898
Bse3DI GCAATG 1 cut(s) 48
BseBI CCWGG 3 cut(s) 469, 545, 1433
BseDI CCNNGG 9 cut(s) 55, 135, 397, 507, 543, 788, 958, 1336, 1481
BseGI GGATG 2 cut(s) 34, 1367
BseLI CCNNNNNNNGG 3 cut(s) 179, 548, 1525
BseMI GCAATG 1 cut(s) 48
BseMII CTCAG 2 cut(s) 617, 967
BseNI ACTGG 4 cut(s) 497, 638, 708, 898
BseRI GAGGAG 2 cut(s) 1384, 1541
BseSI GKGCMC 1 cut(s) 826
BseX3I CGGCCG 1 cut(s) 1095
BseXI GCAGC 5 cut(s) 42, 850, 895, 1159, 1162
BsgI GTGCAG 1 cut(s) 233
Bsh1236I CGCG 1 cut(s) 916
Bsh1285I CGRYCG 1 cut(s) 1098
BshFI GGCC 5 cut(s) 134, 458, 730, 767, 1097
BshNI GGYRCC 1 cut(s) 821
BshTI ACCGGT 1 cut(s) 1557
BsiEI CGRYCG 1 cut(s) 1098
BsiHKAI GWGCWC 2 cut(s) 1061, 1141
BsiHKCI CYCGRG 1 cut(s) 1301
BsiSI CCGG 4 cut(s) 727, 1098, 1359, 1558
BslFI GGGAC 3 cut(s) 296, 1021, 1526
BslI CCNNNNNNNGG 3 cut(s) 179, 548, 1525
BsmAI GTCTC 2 cut(s) 1033, 1461
BsmFI GGGAC 3 cut(s) 296, 1021, 1526
BsmI GAATGC 3 cut(s) 302, 570, 1482
BsnI GGCC 5 cut(s) 134, 458, 730, 767, 1097
BsoBI CYCGRG 1 cut(s) 1301
Bsp1286I GDGCHC 4 cut(s) 406, 826, 1061, 1141
Bsp143I GATC 2 cut(s) 153, 882
Bsp19I CCATGG 2 cut(s) 788, 1336
BspACI CCGC 4 cut(s) 95, 482, 705, 1519
BspANI GGCC 5 cut(s) 134, 458, 730, 767, 1097
BspCNI CTCAG 2 cut(s) 616, 966
BspFNI CGCG 1 cut(s) 916
BspHI TCATGA 1 cut(s) 331
BspLI GGNNCC 6 cut(s) 116, 268, 312, 495, 823, 1256
BspPI GGATC 1 cut(s) 148
BspQI GCTCTTC 1 cut(s) 663
BspT107I GGYRCC 1 cut(s) 821
BsrBI CCGCTC 1 cut(s) 484
BsrDI GCAATG 1 cut(s) 48
BsrFI RCCGGY 3 cut(s) 726, 1097, 1557
BsrI ACTGG 4 cut(s) 497, 638, 708, 898
BssAI RCCGGY 3 cut(s) 726, 1097, 1557
BssECI CCNNGG 9 cut(s) 55, 135, 397, 507, 543, 788, 958, 1336, 1481
BssMI GATC 2 cut(s) 153, 882
BssSI CACGAG 1 cut(s) 1062
BssT1I CCWWGG 6 cut(s) 55, 135, 397, 507, 788, 1336
Bst2BI CACGAG 1 cut(s) 1062
Bst2UI CCWGG 3 cut(s) 469, 545, 1433
Bst4CI ACNGT 5 cut(s) 283, 676, 1027, 1408, 1460
Bst6I CTCTTC 3 cut(s) 15, 663, 816
BstC8I GCNNGC 2 cut(s) 482, 971
BstDEI CTNAG 3 cut(s) 61, 603, 953
BstDSI CCRYGG 4 cut(s) 788, 958, 1336, 1481
BstF5I GGATG 2 cut(s) 34, 1367
BstFNI CGCG 1 cut(s) 916
BstKTI GATC 2 cut(s) 156, 885
BstMAI GTCTC 2 cut(s) 1033, 1461
BstMBI GATC 2 cut(s) 153, 882
BstMCI CGRYCG 1 cut(s) 1098
BstMWI GCNNNNNNNGC 3 cut(s) 713, 905, 1145
BstNI CCWGG 3 cut(s) 469, 545, 1433
BstSCI CCNGG 3 cut(s) 467, 543, 1431
BstSFI CTRYAG 1 cut(s) 1032
BstSLI GKGCMC 1 cut(s) 826
BstUI CGCG 1 cut(s) 916
BstV1I GCAGC 5 cut(s) 42, 850, 895, 1159, 1162
BstZI CGGCCG 1 cut(s) 1095
BsuRI GGCC 5 cut(s) 134, 458, 730, 767, 1097
BtgI CCRYGG 4 cut(s) 788, 958, 1336, 1481
BtgZI GCGATG 1 cut(s) 897
BtsCI GGATG 2 cut(s) 34, 1367
BtsIMutI CAGTG 3 cut(s) 681, 715, 1465
Cac8I GCNNGC 2 cut(s) 482, 971
CciI TCATGA 1 cut(s) 331
Cfr10I RCCGGY 3 cut(s) 726, 1097, 1557
Cfr13I GGNCC 5 cut(s) 46, 310, 456, 765, 1340
CseI GACGC 1 cut(s) 905
Csp6I GTAC 4 cut(s) 67, 607, 1306, 1450
CspAI ACCGGT 1 cut(s) 1557
CspCI CAANNNNNGTGG 2 cut(s) 148, 183
CviQI GTAC 4 cut(s) 67, 607, 1306, 1450
DdeI CTNAG 3 cut(s) 61, 603, 953
DpnI GATC 2 cut(s) 155, 884
DpnII GATC 2 cut(s) 153, 882
EaeI YGGCCR 2 cut(s) 728, 1095
EagI CGGCCG 1 cut(s) 1095
Eam1104I CTCTTC 3 cut(s) 15, 663, 816
EarI CTCTTC 3 cut(s) 15, 663, 816
EciI GGCGGA 1 cut(s) 110
EclXI CGGCCG 1 cut(s) 1095
Eco130I CCWWGG 6 cut(s) 55, 135, 397, 507, 788, 1336
Eco24I GRGCYC 1 cut(s) 406
Eco47I GGWCC 3 cut(s) 46, 310, 1340
Eco52I CGGCCG 1 cut(s) 1095
Eco57I CTGAAG 2 cut(s) 460, 1294
Eco88I CYCGRG 1 cut(s) 1301
EcoRII CCWGG 3 cut(s) 467, 543, 1431
EcoT14I CCWWGG 6 cut(s) 55, 135, 397, 507, 788, 1336
EcoT38I GRGCYC 1 cut(s) 406
ErhI CCWWGG 6 cut(s) 55, 135, 397, 507, 788, 1336
FaqI GGGAC 3 cut(s) 296, 1021, 1526
FauI CCCGC 1 cut(s) 489
FauNDI CATATG 1 cut(s) 688
Fnu4HI GCNGC 6 cut(s) 31, 864, 909, 1173, 1176, 1519
FokI GGATG 2 cut(s) 21, 1374
FriOI GRGCYC 1 cut(s) 406
Fsp4HI GCNGC 6 cut(s) 31, 864, 909, 1173, 1176, 1519
FspBI CTAG 1 cut(s) 920
GluI GCNGC 6 cut(s) 31, 864, 909, 1173, 1176, 1519
GsuI CTGGAG 2 cut(s) 915, 924
HaeIII GGCC 5 cut(s) 134, 458, 730, 767, 1097
HapII CCGG 4 cut(s) 727, 1098, 1359, 1558
HgaI GACGC 1 cut(s) 905
HincII GTYRAC 1 cut(s) 891
HindII GTYRAC 1 cut(s) 891
HinfI GANTC 8 cut(s) 77, 217, 237, 335, 372, 887, 984, 999
HpaII CCGG 4 cut(s) 727, 1098, 1359, 1558
HphI GGTGA 3 cut(s) 197, 206, 1153
Hpy166II GTNNAC 1 cut(s) 891
Hpy188I TCNGA 3 cut(s) 408, 488, 1380
Hpy188III TCNNGA 4 cut(s) 313, 332, 700, 1301
Hpy8I GTNNAC 1 cut(s) 891
Hpy99I CGWCG 1 cut(s) 1252
HpyAV CCTTC 4 cut(s) 343, 1168, 1321, 1555
HpyCH4III ACNGT 5 cut(s) 283, 676, 1027, 1408, 1460
HpyCH4IV ACGT 1 cut(s) 1010
HpyCH4V TGCA 9 cut(s) 72, 214, 626, 692, 716, 911, 1178, 1228, 1463
HpyF10VI GCNNNNNNNGC 3 cut(s) 713, 905, 1145
HpyF3I CTNAG 3 cut(s) 61, 603, 953
HpySE526I ACGT 1 cut(s) 1010
Kzo9I GATC 2 cut(s) 153, 882
LguI GCTCTTC 1 cut(s) 663
LmnI GCTCC 4 cut(s) 13, 790, 896, 1056
Lsp1109I GCAGC 5 cut(s) 42, 850, 895, 1159, 1162
LweI GCATC 2 cut(s) 920, 1157
MaeI CTAG 1 cut(s) 920
MaeII ACGT 1 cut(s) 1010
MaeIII GTNAC 2 cut(s) 194, 1316
MalI GATC 2 cut(s) 155, 884
MbiI CCGCTC 1 cut(s) 484
MboI GATC 2 cut(s) 153, 882
MboII GAAGA 9 cut(s) 32, 466, 650, 771, 803, 1105, 1273, 1381, 1555
MfeI CAATTG 2 cut(s) 102, 748
MhlI GDGCHC 4 cut(s) 406, 826, 1061, 1141
MlyI GAGTC 4 cut(s) 71, 211, 896, 993
MmeI TCCRAC 3 cut(s) 529, 1048, 1427
MroXI GAANNNNTTC 1 cut(s) 1331
MseI TTAA 2 cut(s) 1554, 1576
MslI CAYNNNNRTG 3 cut(s) 575, 1238, 1353
MspI CCGG 4 cut(s) 727, 1098, 1359, 1558
MspR9I CCNGG 3 cut(s) 469, 545, 1433
MunI CAATTG 2 cut(s) 102, 748
Mva1269I GAATGC 3 cut(s) 302, 570, 1482
MvaI CCWGG 3 cut(s) 469, 545, 1433
MvnI CGCG 1 cut(s) 916
MwoI GCNNNNNNNGC 3 cut(s) 713, 905, 1145
NcoI CCATGG 2 cut(s) 788, 1336
NdeI CATATG 1 cut(s) 688
NdeII GATC 2 cut(s) 153, 882
NlaIV GGNNCC 6 cut(s) 116, 268, 312, 495, 823, 1256
NmeAIII GCCGAG 1 cut(s) 1073
NmuCI GTSAC 1 cut(s) 194
PagI TCATGA 1 cut(s) 331
PciSI GCTCTTC 1 cut(s) 663
PctI GAATGC 3 cut(s) 302, 570, 1482
PdmI GAANNNNTTC 1 cut(s) 1331
PfeI GAWTC 4 cut(s) 237, 335, 372, 984
PflFI GACNNNGTC 1 cut(s) 1012
PfoI TCCNGGA 1 cut(s) 1431
PinAI ACCGGT 1 cut(s) 1557
PkrI GCNGC 6 cut(s) 32, 865, 910, 1174, 1177, 1520
PleI GAGTC 4 cut(s) 71, 211, 895, 993
PpsI GAGTC 4 cut(s) 71, 211, 895, 993
Psp6I CCWGG 3 cut(s) 467, 543, 1431
PspGI CCWGG 3 cut(s) 467, 543, 1431
PspN4I GGNNCC 6 cut(s) 116, 268, 312, 495, 823, 1256
PspPI GGNCC 5 cut(s) 46, 310, 456, 765, 1340
PsyI GACNNNGTC 1 cut(s) 1012
RsaI GTAC 4 cut(s) 68, 608, 1307, 1451
RsaNI GTAC 4 cut(s) 67, 607, 1306, 1450
RseI CAYNNNNRTG 3 cut(s) 575, 1238, 1353
SapI GCTCTTC 1 cut(s) 663
SaqAI TTAA 2 cut(s) 1554, 1576
SatI GCNGC 6 cut(s) 31, 864, 909, 1173, 1176, 1519
Sau3AI GATC 2 cut(s) 153, 882
Sau96I GGNCC 5 cut(s) 46, 310, 456, 765, 1340
ScaI AGTACT 2 cut(s) 68, 1307
SchI GAGTC 4 cut(s) 71, 211, 896, 993
ScrFI CCNGG 3 cut(s) 469, 545, 1433
SduI GDGCHC 4 cut(s) 406, 826, 1061, 1141
SfaNI GCATC 2 cut(s) 920, 1157
SfcI CTRYAG 1 cut(s) 1032
SinI GGWCC 3 cut(s) 46, 310, 1340
SmiMI CAYNNNNRTG 3 cut(s) 575, 1238, 1353
SmlI CTYRAG 1 cut(s) 1445
SmoI CTYRAG 1 cut(s) 1445
SsiI CCGC 4 cut(s) 95, 482, 705, 1519
SspMI CTAG 1 cut(s) 920
StyD4I CCNGG 3 cut(s) 467, 543, 1431
StyI CCWWGG 6 cut(s) 55, 135, 397, 507, 788, 1336
TaaI ACNGT 5 cut(s) 283, 676, 1027, 1408, 1460
TaiI ACGT 1 cut(s) 1013
TaqI TCGA 2 cut(s) 885, 1247
TatI WGTACW 3 cut(s) 66, 1305, 1449
TauI GCSGC 1 cut(s) 1521
TfiI GAWTC 4 cut(s) 237, 335, 372, 984
Tru1I TTAA 2 cut(s) 1554, 1576
Tru9I TTAA 2 cut(s) 1554, 1576
TscAI CASTG 3 cut(s) 681, 715, 1465
TseFI GTSAC 1 cut(s) 194
TseI GCWGC 5 cut(s) 30, 863, 908, 1172, 1175
Tsp45I GTSAC 1 cut(s) 194
TspDTI ATGAA 6 cut(s) 166, 295, 320, 362, 867, 1256
TspRI CASTG 3 cut(s) 681, 715, 1465
Tth111I GACNNNGTC 1 cut(s) 1012
VpaK11BI GGWCC 3 cut(s) 46, 310, 1340
XapI RAATTY 3 cut(s) 450, 1130, 1493
XmnI GAANNNNTTC 1 cut(s) 1331
XspI CTAG 1 cut(s) 920
ZrmI AGTACT 2 cut(s) 68, 1307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.