Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0009827

Physical Location & Seq

Genomic Coordinates Forward (+)

43227443 .. 43229267

Gene Structure 1825 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1298800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1497 bp

ATGGCAGACCTCAAACCCAAAACCACCGACAACGGCAGCACTTCCACCCCCTCAAGCCCAACCACCGCCACATCTCCCACCCGCACGACCAGCAAATCCAAAAACTGCCCTGCTCTGCTTCTGGGTCGCTTCGAAATCGGCAAACTCCTCGGCCATGGCACCTTCGCCAAAGTATACCTCGCCAAAAACGTCAAAACCAACGAGAGCGTCGCAATCAAAGTCATCGACAAGGAGAAGATCCTCAAGGGCGGTTTGATCGCTCACATAAAGCGGGAGATCTCGATCCTCCGCCGCGTTCGCCACCCGAACATCGTCCAGCTTTTCGAGGTCATGGCCACGAAAGCCAAGATCTATTTCGTGATGGAATACGTCCGCGGCGGCGAGCTCTTTAACAAGGTCGCAAAGGGCCGGCTGAAGGAGGAAGTGGCGAGGAAGTACTTCCAGCAGCTAGTCTCCGCCGTCGGATTCTGCCACGCCCGCGGCGTCTATCACCGCGATTTGAAGCCGGAGAACCTATTGCTCGACGAGAACGGAGACCTCAAGGTTTCCGATTTTGGGTTGAGCGCGGTTTCTGATGAAATTCGACAAGATGGTCTCTTTCACACGTTTTGCGGCACGCCGGCGTACGTCGCCCCGGAGGTTTTGGGCCGGAAAGGGTACGACGCCGCGAAGGTGGATATTTGGTCTTGTGGGGTTGTTTTGTTTGTGCTCATGGCTGGATATTTACCCTTCCATGACCAGAATGTTATGGCGATGTACAAGAAGATCTACAAAGGCGAATTTCGATGCCCCAGATGGTTCAGTGCCGAGCTTGTTAGGCTTCTATCCCGCCTTCTCGACACTAATCCGGAGTCGAGGATTACAATCCCGGATGTTATGGAGAATCGGTGGTTTAAAAAGGGTTTTAAGCACATCAAGTTTTACATTGATCACGATGATCGGCTCTGCAATGTTGTTGATGATGATGAGGAGGACAGGTTTAGTGATGTGAGCTCTGTTTCAGATTGTATGTCGGAATCTGAGGCGGAGTTTGAGACCAGAAGAAAGATTACATCTTTACCCAGACCGGCTAGCCTGAATGCGTTTGATATAATCTCGTTTGCCCCCGGGTTTGATTTGTCCGGGTTGTTTGAGGAGCGGGGAGAGGAGCCGAGGTTTGTGTCGGGCGCCCCTGTTTCGACCATCATATCGAAATTGGAGGAGATTGCGAAAGTCGTGAGCTTTTCGGTGAGGAAGAAGGATTGCAGAGTGAGCTTGGAAGGGTCTAGGGAAGGTGTTAAGGGGCCATTGACAATTGCGGCTGAGATATTCGAACTTACGCCTAAGTTGGTGGTGTTGGAGGTTAAGAAGAAAGCAGGGGACAGAGCTGAGTACGAGCAGTTTTGTAATACCGAATTGAGACCGGGGTTGCAGAACTTGATGATTGAGGAGAGTGCTGCTGGTGCTACCGCTGCTGCTCCCGCTCCCGCTCCTTATCTACCTTCGGATACTGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018107 GO:0018193 GO:0018209 GO:0018210 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04068 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418

Pfam Domains

Protein Families

NAF Pkinase

Protein Analysis

499

Amino Acids

55.57

Weight (kDa)

8.21

Isoelectric Point (pI)

39.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	44 - 298	2.7e-76	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	45 - 294	4.3e-38	Protein tyrosine and serine/threonine kinase
Kinase-like	PF14531	138 - 286	2.4e-06	Kinase-like
NAF	PF03822	356 - 412	5.7e-22	NAF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009827)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29230 AT2G34180 AT4G18700 AT5G45810
fragaria_vesca	FvH4_3g07620 FvH4_3g07620
malus_domestica	MD05G1298800.v1.1 MD10G1278300.v1.1
prunus_persica	Prupe.4G063100_v2.0.a1
pyrus_communis	pycom10g23220
rosa_chinensis	RchiOBHm_Chr5g0011071
rosa_laevigata	RLG00000031807
rosa_multiflora	Rmu_co8374009.1_g000001 Rmu_sc0003242.1_g000008
rosa_roxburghii	Rroxscaffold_1G00065340
rosa_rugosa	Rorug04G0455000
rosa_samantha	Rh5AG087200 Rh5BG082000 Rh5CG094800
rosa_wichuraiana	Rw0G000160 Rw0G017790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	591
AccB1I	GGYRCC	2 cut(s)	158, 1166
AccBSI	CCGCTC	3 cut(s)	1138, 1463, 1469
AccI	GTMKAC	1 cut(s)	174
AccII	CGCG	6 cut(s)	294, 375, 480, 495, 566, 668
AccIII	TCCGGA	1 cut(s)	847
AclWI	GGATC	2 cut(s)	232, 277
AcoI	YGGCCR	2 cut(s)	151, 333
AcsI	RAATTY	2 cut(s)	579, 779
AcuI	CTGAAG	1 cut(s)	434
AcyI	GRCGYC	3 cut(s)	483, 663, 1167
AfaI	GTAC	5 cut(s)	437, 626, 659, 758, 1373
AfiI	CCNNNNNNNGG	2 cut(s)	415, 555
AflIII	ACRYGT	1 cut(s)	603
AgsI	TTSAA	1 cut(s)	502
AjuI	GAANNNNNNNTTGG	2 cut(s)	338, 370
AluBI	AGCT	8 cut(s)	319, 385, 448, 811, 993, 1221, 1254, 1367
AluI	AGCT	8 cut(s)	319, 385, 448, 811, 993, 1221, 1254, 1367
Alw21I	GWGCWC	3 cut(s)	387, 711, 995
Alw26I	GTCTC	5 cut(s)	457, 528, 599, 1028, 1393
AlwI	GGATC	2 cut(s)	232, 277
Ama87I	CYCGRG	1 cut(s)	1106
Aor13HI	TCCGGA	1 cut(s)	847
AoxI	GGCC	5 cut(s)	151, 333, 406, 646, 1283
ApeKI	GCWGC	5 cut(s)	36, 445, 1436, 1451, 1454
ApoI	RAATTY	2 cut(s)	579, 779
Asp700I	GAANNNNTTC	1 cut(s)	437
AspLEI	GCGC	2 cut(s)	566, 1169
AspS9I	GGNCC	3 cut(s)	406, 646, 1283
AsuC2I	CCSGG	6 cut(s)	635, 869, 1107, 1108, 1123, 1404
AsuHPI	GGTGA	2 cut(s)	482, 1240
AsuII	TTCGAA	2 cut(s)	132, 1311
AsuNHI	GCTAGC	1 cut(s)	1070
AvaI	CYCGRG	1 cut(s)	1106
BalI	TGGCCA	1 cut(s)	335
BanI	GGYRCC	2 cut(s)	158, 1166
BanII	GRGCYC	2 cut(s)	387, 995
BarI	GAAGNNNNNNTAC	2 cut(s)	1033, 1065
Bbv12I	GWGCWC	3 cut(s)	387, 711, 995
BbvI	GCAGC	5 cut(s)	48, 457, 1423, 1438, 1441
BccI	CCATC	4 cut(s)	355, 584, 789, 1190
BceAI	ACGGC	2 cut(s)	49, 443
BcgI	CGANNNNNNTGC	2 cut(s)	130, 164
BciVI	GTATCC	1 cut(s)	1480
BclI	TGATCA	1 cut(s)	928
BcnI	CCSGG	6 cut(s)	635, 869, 1107, 1108, 1123, 1404
BcoDI	GTCTC	5 cut(s)	457, 528, 599, 1028, 1393
BfaI	CTAG	3 cut(s)	449, 1071, 1266
BfoI	RGCGCY	1 cut(s)	1170
BfuI	GTATCC	1 cut(s)	1480
BglII	AGATCT	3 cut(s)	276, 348, 765
BmcAI	AGTACT	1 cut(s)	437
Bme1390I	CCNGG	6 cut(s)	635, 869, 1107, 1108, 1123, 1404
BmeT110I	CYCGRG	1 cut(s)	1106
BmgT120I	GGNCC	3 cut(s)	406, 646, 1283
BmiI	GGNNCC	4 cut(s)	160, 1149, 1168, 1284
BmrFI	CCNGG	6 cut(s)	635, 869, 1107, 1108, 1123, 1404
BmsI	GCATC	1 cut(s)	776
BmtI	GCTAGC	1 cut(s)	1074
Bpu14I	TTCGAA	2 cut(s)	132, 1311
BpuEI	CTTGAG	3 cut(s)	37, 227, 524
BpuMI	CCSGG	6 cut(s)	635, 869, 1107, 1108, 1123, 1404
BsaBI	GATNNNNATC	2 cut(s)	281, 863
BsaHI	GRCGYC	3 cut(s)	483, 663, 1167
BsaI	GGTCTC	4 cut(s)	528, 599, 1028, 1393
BsaJI	CCNNGG	9 cut(s)	148, 154, 373, 478, 633, 1105, 1106, 1151, 1403
BsaWI	WCCGGW	1 cut(s)	847
BsaXI	ACNNNNNCTCC	6 cut(s)	872, 902, 962, 992, 1139, 1169
Bsc4I	CCNNNNNNNGG	2 cut(s)	415, 555
Bse118I	RCCGGY	3 cut(s)	408, 619, 1066
Bse3DI	GCAATG	1 cut(s)	955
Bse8I	GATNNNNATC	2 cut(s)	281, 863
BseAI	TCCGGA	1 cut(s)	847
BseDI	CCNNGG	9 cut(s)	148, 154, 373, 478, 633, 1105, 1106, 1151, 1403
BseGI	GGATG	1 cut(s)	877
BseJI	GATNNNNATC	2 cut(s)	281, 863
BseLI	CCNNNNNNNGG	2 cut(s)	415, 555
BseMI	GCAATG	1 cut(s)	955
BseMII	CTCAG	3 cut(s)	1011, 1293, 1359
BseRI	GAGGAG	6 cut(s)	137, 983, 1148, 1160, 1214, 1442
BseXI	GCAGC	5 cut(s)	48, 457, 1423, 1438, 1441
Bsh1236I	CGCG	6 cut(s)	294, 375, 480, 495, 566, 668
BshFI	GGCC	5 cut(s)	153, 335, 408, 648, 1285
BshNI	GGYRCC	2 cut(s)	158, 1166
BsiHKAI	GWGCWC	3 cut(s)	387, 711, 995
BsiHKCI	CYCGRG	1 cut(s)	1106
BsiWI	CGTACG	1 cut(s)	624
BslFI	GGGAC	1 cut(s)	1373
BslI	CCNNNNNNNGG	2 cut(s)	415, 555
BsmAI	GTCTC	5 cut(s)	457, 528, 599, 1028, 1393
BsmFI	GGGAC	1 cut(s)	1373
BsmI	GAATGC	1 cut(s)	1084
BsnI	GGCC	5 cut(s)	153, 335, 408, 648, 1285
Bso31I	GGTCTC	4 cut(s)	528, 599, 1028, 1393
BsoBI	CYCGRG	1 cut(s)	1106
Bsp119I	TTCGAA	2 cut(s)	132, 1311
Bsp1286I	GDGCHC	3 cut(s)	387, 711, 995
Bsp13I	TCCGGA	1 cut(s)	847
Bsp1407I	TGTACA	1 cut(s)	756
Bsp143I	GATC	8 cut(s)	237, 255, 276, 282, 348, 765, 928, 937
Bsp19I	CCATGG	1 cut(s)	154
BspANI	GGCC	5 cut(s)	153, 335, 408, 648, 1285
BspCNI	CTCAG	3 cut(s)	1012, 1294, 1360
BspEI	TCCGGA	1 cut(s)	847
BspFNI	CGCG	6 cut(s)	294, 375, 480, 495, 566, 668
BspLI	GGNNCC	4 cut(s)	160, 1149, 1168, 1284
BspOI	GCTAGC	1 cut(s)	1074
BspPI	GGATC	2 cut(s)	232, 277
BspT104I	TTCGAA	2 cut(s)	132, 1311
BspT107I	GGYRCC	2 cut(s)	158, 1166
BspTNI	GGTCTC	4 cut(s)	528, 599, 1028, 1393
BsrBI	CCGCTC	3 cut(s)	1138, 1463, 1469
BsrDI	GCAATG	1 cut(s)	955
BsrFI	RCCGGY	3 cut(s)	408, 619, 1066
BsrGI	TGTACA	1 cut(s)	756
BssAI	RCCGGY	3 cut(s)	408, 619, 1066
BssECI	CCNNGG	9 cut(s)	148, 154, 373, 478, 633, 1105, 1106, 1151, 1403
BssMI	GATC	8 cut(s)	237, 255, 276, 282, 348, 765, 928, 937
BssNAI	GTATAC	1 cut(s)	175
BssNI	GRCGYC	3 cut(s)	483, 663, 1167
BssT1I	CCWWGG	1 cut(s)	154
Bst1107I	GTATAC	1 cut(s)	175
BstACI	GRCGYC	3 cut(s)	483, 663, 1167
BstAUI	TGTACA	1 cut(s)	756
BstBI	TTCGAA	2 cut(s)	132, 1311
BstC8I	GCNNGC	6 cut(s)	383, 410, 478, 617, 621, 1072
BstDEI	CTNAG	4 cut(s)	1020, 1302, 1323, 1368
BstDSI	CCRYGG	3 cut(s)	154, 373, 478
BstF5I	GGATG	1 cut(s)	877
BstFNI	CGCG	6 cut(s)	294, 375, 480, 495, 566, 668
BstH2I	RGCGCY	1 cut(s)	1170
BstHHI	GCGC	2 cut(s)	566, 1169
BstKTI	GATC	8 cut(s)	240, 258, 279, 285, 351, 768, 931, 940
BstMAI	GTCTC	5 cut(s)	457, 528, 599, 1028, 1393
BstMBI	GATC	8 cut(s)	237, 255, 276, 282, 348, 765, 928, 937
BstSCI	CCNGG	6 cut(s)	633, 867, 1105, 1106, 1121, 1402
BstUI	CGCG	6 cut(s)	294, 375, 480, 495, 566, 668
BstV1I	GCAGC	5 cut(s)	48, 457, 1423, 1438, 1441
BstX2I	RGATCY	4 cut(s)	237, 276, 348, 765
BstYI	RGATCY	4 cut(s)	237, 276, 348, 765
BstZ17I	GTATAC	1 cut(s)	175
BsuI	GTATCC	1 cut(s)	1480
BsuRI	GGCC	5 cut(s)	153, 335, 408, 648, 1285
BtgI	CCRYGG	3 cut(s)	154, 373, 478
BtgZI	GCGATG	1 cut(s)	767
BtsCI	GGATG	1 cut(s)	877
BtsIMutI	CAGTG	1 cut(s)	808
Cac8I	GCNNGC	6 cut(s)	383, 410, 478, 617, 621, 1072
CfoI	GCGC	2 cut(s)	566, 1169
Cfr10I	RCCGGY	3 cut(s)	408, 619, 1066
Cfr13I	GGNCC	3 cut(s)	406, 646, 1283
Cfr42I	CCGCGG	2 cut(s)	376, 481
Cfr9I	CCCGGG	1 cut(s)	1106
CseI	GACGC	3 cut(s)	196, 472, 671
Csp6I	GTAC	5 cut(s)	436, 625, 658, 757, 1372
CviAII	CATG	4 cut(s)	155, 331, 712, 734
CviQI	GTAC	5 cut(s)	436, 625, 658, 757, 1372
DdeI	CTNAG	4 cut(s)	1020, 1302, 1323, 1368
DinI	GGCGCC	1 cut(s)	1168
DpnI	GATC	8 cut(s)	239, 257, 278, 284, 350, 767, 930, 939
DpnII	GATC	8 cut(s)	237, 255, 276, 282, 348, 765, 928, 937
DraI	TTTAAA	1 cut(s)	895
DrdI	GACNNNNNNGTC	1 cut(s)	591
DseDI	GACNNNNNNGTC	1 cut(s)	591
EaeI	YGGCCR	2 cut(s)	151, 333
EciI	GGCGGA	3 cut(s)	278, 445, 1040
Ecl136II	GAGCTC	2 cut(s)	385, 993
Eco130I	CCWWGG	1 cut(s)	154
Eco24I	GRGCYC	2 cut(s)	387, 995
Eco31I	GGTCTC	4 cut(s)	528, 599, 1028, 1393
Eco53kI	GAGCTC	2 cut(s)	385, 993
Eco57I	CTGAAG	1 cut(s)	434
Eco88I	CYCGRG	1 cut(s)	1106
EcoICRI	GAGCTC	2 cut(s)	385, 993
EcoT14I	CCWWGG	1 cut(s)	154
EcoT38I	GRGCYC	2 cut(s)	387, 995
EgeI	GGCGCC	1 cut(s)	1168
EheI	GGCGCC	1 cut(s)	1168
ErhI	CCWWGG	1 cut(s)	154
FaeI	CATG	4 cut(s)	158, 334, 715, 737
FaqI	GGGAC	1 cut(s)	1373
FatI	CATG	4 cut(s)	154, 330, 711, 733
FauI	CCCGC	7 cut(s)	89, 264, 485, 836, 1131, 1468, 1474
FbaI	TGATCA	1 cut(s)	928
FblI	GTMKAC	1 cut(s)	174
FokI	GGATG	1 cut(s)	884
FriOI	GRGCYC	2 cut(s)	387, 995
FspBI	CTAG	3 cut(s)	449, 1071, 1266
GlaI	GCGC	2 cut(s)	565, 1168
HaeII	RGCGCY	1 cut(s)	1170
HaeIII	GGCC	5 cut(s)	153, 335, 408, 648, 1285
HgaI	GACGC	3 cut(s)	196, 472, 671
HhaI	GCGC	2 cut(s)	566, 1169
Hin1I	GRCGYC	3 cut(s)	483, 663, 1167
Hin1II	CATG	4 cut(s)	158, 334, 715, 737
Hin6I	GCGC	2 cut(s)	564, 1167
HinP1I	GCGC	2 cut(s)	564, 1167
HinfI	GANTC	4 cut(s)	465, 851, 883, 1016
HphI	GGTGA	2 cut(s)	482, 1240
Hpy166II	GTNNAC	1 cut(s)	175
Hpy188I	TCNGA	7 cut(s)	464, 550, 574, 1003, 1015, 1021, 1486
Hpy188III	TCNNGA	6 cut(s)	280, 358, 836, 848, 932, 1216
Hpy8I	GTNNAC	1 cut(s)	175
Hpy99I	CGWCG	5 cut(s)	212, 464, 527, 632, 665
HpyAV	CCTTC	9 cut(s)	172, 409, 664, 739, 842, 1231, 1253, 1265, 1491
HpyCH4IV	ACGT	4 cut(s)	189, 369, 605, 627
HpyCH4V	TGCA	3 cut(s)	948, 1245, 1411
HpyF3I	CTNAG	4 cut(s)	1020, 1302, 1323, 1368
HpySE526I	ACGT	4 cut(s)	189, 369, 605, 627
Hsp92I	GRCGYC	3 cut(s)	483, 663, 1167
Hsp92II	CATG	4 cut(s)	158, 334, 715, 737
HspAI	GCGC	2 cut(s)	564, 1167
KasI	GGCGCC	1 cut(s)	1166
Kpn2I	TCCGGA	1 cut(s)	847
KroI	GCCGGC	2 cut(s)	408, 619
KroNI	GCCGGC	2 cut(s)	410, 621
Ksp22I	TGATCA	1 cut(s)	928
KspI	CCGCGG	2 cut(s)	376, 481
Kzo9I	GATC	8 cut(s)	237, 255, 276, 282, 348, 765, 928, 937
LmnI	GCTCC	5 cut(s)	1135, 1147, 1462, 1468, 1474
Lsp1109I	GCAGC	5 cut(s)	48, 457, 1423, 1438, 1441
LweI	GCATC	1 cut(s)	776
MaeI	CTAG	3 cut(s)	449, 1071, 1266
MaeII	ACGT	4 cut(s)	189, 369, 605, 627
MalI	GATC	8 cut(s)	239, 257, 278, 284, 350, 767, 930, 939
MbiI	CCGCTC	3 cut(s)	1138, 1463, 1469
MboI	GATC	8 cut(s)	237, 255, 276, 282, 348, 765, 928, 937
MboII	GAAGA	5 cut(s)	247, 775, 1053, 1246, 1360
MfeI	CAATTG	1 cut(s)	1293
MflI	RGATCY	4 cut(s)	237, 276, 348, 765
MhlI	GDGCHC	3 cut(s)	387, 711, 995
MlsI	TGGCCA	1 cut(s)	335
MluCI	AATT	5 cut(s)	579, 779, 1193, 1293, 1394
MluNI	TGGCCA	1 cut(s)	335
Mly113I	GGCGCC	1 cut(s)	1167
MlyI	GAGTC	1 cut(s)	860
MmeI	TCCRAC	3 cut(s)	442, 993, 1317
Mox20I	TGGCCA	1 cut(s)	335
MreI	CGCCGGCG	1 cut(s)	619
MroI	TCCGGA	1 cut(s)	847
MroNI	GCCGGC	2 cut(s)	408, 619
MroXI	GAANNNNTTC	1 cut(s)	437
MscI	TGGCCA	1 cut(s)	335
MseI	TTAA	5 cut(s)	390, 894, 906, 1278, 1344
Msp20I	TGGCCA	1 cut(s)	335
MspA1I	CMGCKG	3 cut(s)	375, 480, 1451
MspR9I	CCNGG	6 cut(s)	635, 869, 1107, 1108, 1123, 1404
MunI	CAATTG	1 cut(s)	1293
Mva1269I	GAATGC	1 cut(s)	1084
MvnI	CGCG	6 cut(s)	294, 375, 480, 495, 566, 668
NaeI	GCCGGC	2 cut(s)	410, 621
NarI	GGCGCC	1 cut(s)	1167
NciI	CCSGG	6 cut(s)	635, 869, 1107, 1108, 1123, 1404
NcoI	CCATGG	1 cut(s)	154
NdeII	GATC	8 cut(s)	237, 255, 276, 282, 348, 765, 928, 937
NgoMIV	GCCGGC	2 cut(s)	408, 619
NheI	GCTAGC	1 cut(s)	1070
NlaIII	CATG	4 cut(s)	158, 334, 715, 737
NlaIV	GGNNCC	4 cut(s)	160, 1149, 1168, 1284
NmeAIII	GCCGAG	3 cut(s)	129, 832, 1176
NspV	TTCGAA	2 cut(s)	132, 1311
PcsI	WCGNNNNNNNCGW	2 cut(s)	186, 480
PctI	GAATGC	1 cut(s)	1084
PdiI	GCCGGC	2 cut(s)	410, 621
PdmI	GAANNNNTTC	1 cut(s)	437
PfeI	GAWTC	3 cut(s)	465, 883, 1016
Pfl23II	CGTACG	1 cut(s)	624
PfoI	TCCNGGA	1 cut(s)	867
PleI	GAGTC	1 cut(s)	859
PluTI	GGCGCC	1 cut(s)	1170
PpsI	GAGTC	1 cut(s)	859
Psp124BI	GAGCTC	2 cut(s)	387, 995
PspLI	CGTACG	1 cut(s)	624
PspN4I	GGNNCC	4 cut(s)	160, 1149, 1168, 1284
PspPI	GGNCC	3 cut(s)	406, 646, 1283
PsuI	RGATCY	4 cut(s)	237, 276, 348, 765
RsaI	GTAC	5 cut(s)	437, 626, 659, 758, 1373
RsaNI	GTAC	5 cut(s)	436, 625, 658, 757, 1372
SacI	GAGCTC	2 cut(s)	387, 995
SacII	CCGCGG	2 cut(s)	376, 481
SaqAI	TTAA	5 cut(s)	390, 894, 906, 1278, 1344
Sau3AI	GATC	8 cut(s)	237, 255, 276, 282, 348, 765, 928, 937
Sau96I	GGNCC	3 cut(s)	406, 646, 1283
ScaI	AGTACT	1 cut(s)	437
SchI	GAGTC	1 cut(s)	860
ScrFI	CCNGG	6 cut(s)	635, 869, 1107, 1108, 1123, 1404
SduI	GDGCHC	3 cut(s)	387, 711, 995
SfaNI	GCATC	1 cut(s)	776
SfoI	GGCGCC	1 cut(s)	1168
Sfr303I	CCGCGG	2 cut(s)	376, 481
SfuI	TTCGAA	2 cut(s)	132, 1311
SgrAI	CRCCGGYG	1 cut(s)	619
SgrBI	CCGCGG	2 cut(s)	376, 481
SmaI	CCCGGG	1 cut(s)	1108
SmlI	CTYRAG	3 cut(s)	52, 242, 539
SmoI	CTYRAG	3 cut(s)	52, 242, 539
Sse9I	AATT	5 cut(s)	579, 779, 1193, 1293, 1394
SspDI	GGCGCC	1 cut(s)	1166
SspMI	CTAG	3 cut(s)	449, 1071, 1266
SstI	GAGCTC	2 cut(s)	387, 995
StyD4I	CCNGG	6 cut(s)	633, 867, 1105, 1106, 1121, 1402
StyI	CCWWGG	1 cut(s)	154
TaiI	ACGT	4 cut(s)	192, 372, 608, 630
TasI	AATT	5 cut(s)	579, 779, 1193, 1293, 1394
TatI	WGTACW	2 cut(s)	435, 756
TauI	GCSGC	7 cut(s)	294, 378, 381, 483, 615, 668, 1301
TfiI	GAWTC	3 cut(s)	465, 883, 1016
Tru1I	TTAA	5 cut(s)	390, 894, 906, 1278, 1344
Tru9I	TTAA	5 cut(s)	390, 894, 906, 1278, 1344
TscAI	CASTG	1 cut(s)	808
TseI	GCWGC	5 cut(s)	36, 445, 1436, 1451, 1454
TspDTI	ATGAA	1 cut(s)	591
TspGWI	ACGGA	1 cut(s)	546
TspMI	CCCGGG	1 cut(s)	1106
TspRI	CASTG	1 cut(s)	808
XapI	RAATTY	2 cut(s)	579, 779
XmaI	CCCGGG	1 cut(s)	1106
XmiI	GTMKAC	1 cut(s)	174
XmnI	GAANNNNTTC	1 cut(s)	437
XspI	CTAG	3 cut(s)	449, 1071, 1266
ZrmI	AGTACT	1 cut(s)	437

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.