MD05G1299100.v1.1

Seed dormancy control

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0015490
Physical Location & Seq
Forward (+)
43251050 .. 43251763
714 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1299100.v1.1.491

Sequence Viewer

Length: 714 bp
ATGAGATCCCAGGTGGAGCAGAGGTTCTCTGAGTTTTTCGAGAGGTGGGTTTTTCAGCTCGAGCAGCTCCAGCAGCAACTTCTCAAGTTGTCGGAGGAGACGCGGCAGAACGAGGCGGAGCTTCGGGCTTTGGTGTCGAAAGCCACCAACCTTCACAAGGAGTATTACACCGTGAAGTGGGCTGCTGCTCGGGATGACGTCCTGGCCTTCTTCTGCCCTGTTTGGTCAAGCCCTTTGGAGAATGCGTATTCCTGGGTCACGGGGTGGAAGCCATCAATGCTGTTCCAGCTCGTCGAGTCGCTGAAGAAGACGAGGCTGGTTTCCATGTCGGAGGAGCAGGTGAGGAAGGTTGAGCAGCTGAGGCAGAAGATGAGGTACGAGGAGGATAAGGTGGAGAGGGAAATGGAGAGGCAGCAGGTGGCGATGGCGGACAGGAGGATGGTGGAATTGGCCCGGCTGGCGACCCGGGCGAGGAACGGGGAAGCGGCGGCGGAGCTAGAGGGGCTGGTGAATGTGGCGATGAAGGTGATGCTGAGTGGGTTGGAGGGGGTGATGAAGGCGGCGGATTGTGTGAGGCTCAAGACATTGAAGGGGTTGTTGGATTTGCTGAGTCCATTGCAGAGTGTGGAGTTGTTGGCTGCTACTTGCATGTTGCAGATTAAGTTGAGGGAATGGGGAGGAAGAAATGTGACCAAAATCAAATGCTCTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DOG1

Protein Analysis

238

Amino Acids

27.48

Weight (kDa)

9.01

Isoelectric Point (pI)

45.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DOG1 PF14144 20 - 100 2.4e-23 Seed dormancy control
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015490)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18650
fragaria_vesca FvH4_3g07650
malus_domestica MD05G1073300.v1.1 MD05G1299100.v1.1 MD10G1044600.v1.1 MD14G1136000.v1.1
prunus_persica Prupe.4G062700_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0011031
rosa_laevigata RLG00000031804
rosa_multiflora Rmu_sc0003242.1_g000003
rosa_roxburghii Rroxscaffold_1G00065370
rosa_rugosa Rorug04G0454700
rosa_samantha Rh5BG081600 Rh5DG081400
rosa_wichuraiana Rw0G000130 Rw0G017820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 328, 406
AatII GACGTC 1 cut(s) 201
Acc36I ACCTGC 2 cut(s) 328, 406
AccII CGCG 1 cut(s) 103
AciI CCGC 8 cut(s) 103, 116, 428, 485, 488, 491, 560, 563
AcuI CTGAAG 1 cut(s) 323
AcyI GRCGYC 1 cut(s) 198
AdeI CACNNNGTG 1 cut(s) 264
AfaI GTAC 1 cut(s) 377
AfiI CCNNNNNNNGG 3 cut(s) 157, 177, 471
AgsI TTSAA 1 cut(s) 589
AjnI CCWGG 3 cut(s) 9, 201, 251
AjuI GAANNNNNNNTTGG 2 cut(s) 581, 613
AloI GAACNNNNNNTCC 2 cut(s) 8, 40
AluBI AGCT 6 cut(s) 58, 67, 121, 289, 358, 496
AluI AGCT 6 cut(s) 58, 67, 121, 289, 358, 496
Alw26I GTCTC 1 cut(s) 92
Ama87I CYCGRG 3 cut(s) 59, 189, 465
AoxI GGCC 2 cut(s) 204, 450
ApeKI GCWGC 7 cut(s) 64, 73, 182, 185, 355, 412, 638
AspS9I GGNCC 1 cut(s) 451
AsuC2I CCSGG 3 cut(s) 454, 466, 467
AsuHPI GGTGA 4 cut(s) 352, 520, 538, 562
AvaI CYCGRG 3 cut(s) 59, 189, 465
BarI GAAGNNNNNNTAC 2 cut(s) 359, 391
BbsI GAAGAC 1 cut(s) 314
BbvCI CCTCAGC 1 cut(s) 359
BbvI GCAGC 7 cut(s) 76, 85, 169, 172, 367, 424, 625
BccI CCATC 3 cut(s) 280, 418, 433
BciT130I CCWGG 3 cut(s) 11, 203, 253
BcnI CCSGG 3 cut(s) 454, 466, 467
BcoDI GTCTC 1 cut(s) 92
BfaI CTAG 1 cut(s) 497
BfuAI ACCTGC 2 cut(s) 328, 406
BglI GCCNNNNNGGC 1 cut(s) 458
Bme1390I CCNGG 6 cut(s) 11, 203, 253, 454, 466, 467
BmeT110I CYCGRG 3 cut(s) 59, 189, 465
BmgT120I GGNCC 1 cut(s) 451
BmrFI CCNGG 6 cut(s) 11, 203, 253, 454, 466, 467
BmsI GCATC 1 cut(s) 519
BpiI GAAGAC 1 cut(s) 314
BpmI CTGGAG 1 cut(s) 53
Bpu10I CCTNAGC 1 cut(s) 359
BpuEI CTTGAG 2 cut(s) 68, 563
BpuMI CCSGG 3 cut(s) 454, 466, 467
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 3 cut(s) 9, 252, 465
BsaXI ACNNNNNCTCC 4 cut(s) 8, 38, 230, 260
Bsc4I CCNNNNNNNGG 3 cut(s) 157, 177, 471
Bse3DI GCAATG 1 cut(s) 614
BseBI CCWGG 3 cut(s) 11, 203, 253
BseDI CCNNGG 3 cut(s) 9, 252, 465
BseGI GGATG 2 cut(s) 199, 444
BseLI CCNNNNNNNGG 3 cut(s) 157, 177, 471
BseMI GCAATG 1 cut(s) 614
BseMII CTCAG 4 cut(s) 21, 350, 524, 599
BseRI GAGGAG 3 cut(s) 110, 347, 395
BseXI GCAGC 7 cut(s) 76, 85, 169, 172, 367, 424, 625
Bsh1236I CGCG 1 cut(s) 103
BshFI GGCC 2 cut(s) 206, 452
BsiHKCI CYCGRG 3 cut(s) 59, 189, 465
BsiSI CCGG 2 cut(s) 454, 466
BslI CCNNNNNNNGG 3 cut(s) 157, 177, 471
BsmAI GTCTC 1 cut(s) 92
BsmBI CGTCTC 1 cut(s) 92
BsmI GAATGC 1 cut(s) 247
BsnI GGCC 2 cut(s) 206, 452
BsoBI CYCGRG 3 cut(s) 59, 189, 465
Bsp143I GATC 1 cut(s) 5
BspACI CCGC 8 cut(s) 103, 116, 428, 485, 488, 491, 560, 563
BspANI GGCC 2 cut(s) 206, 452
BspCNI CTCAG 4 cut(s) 22, 351, 525, 600
BspFNI CGCG 1 cut(s) 103
BspMI ACCTGC 2 cut(s) 328, 406
BsrDI GCAATG 1 cut(s) 614
BssECI CCNNGG 3 cut(s) 9, 252, 465
BssMI GATC 1 cut(s) 5
BssNI GRCGYC 1 cut(s) 198
Bst2UI CCWGG 3 cut(s) 11, 203, 253
Bst4CI ACNGT 1 cut(s) 172
BstACI GRCGYC 1 cut(s) 198
BstC8I GCNNGC 1 cut(s) 459
BstDEI CTNAG 4 cut(s) 30, 359, 533, 608
BstENI CCTNNNNNAGG 1 cut(s) 155
BstF5I GGATG 2 cut(s) 199, 444
BstFNI CGCG 1 cut(s) 103
BstKTI GATC 1 cut(s) 8
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 1 cut(s) 5
BstMWI GCNNNNNNNGC 9 cut(s) 64, 70, 73, 277, 286, 361, 458, 467, 502
BstNI CCWGG 3 cut(s) 11, 203, 253
BstNSI RCATGY 1 cut(s) 652
BstSCI CCNGG 6 cut(s) 9, 201, 251, 452, 464, 465
BstUI CGCG 1 cut(s) 103
BstV1I GCAGC 7 cut(s) 76, 85, 169, 172, 367, 424, 625
BstV2I GAAGAC 1 cut(s) 314
BstX2I RGATCY 1 cut(s) 5
BstYI RGATCY 1 cut(s) 5
BsuRI GGCC 2 cut(s) 206, 452
BtgZI GCGATG 2 cut(s) 437, 533
BtsCI GGATG 2 cut(s) 199, 444
BveI ACCTGC 2 cut(s) 328, 406
Cac8I GCNNGC 1 cut(s) 459
Cfr13I GGNCC 1 cut(s) 451
Cfr9I CCCGGG 1 cut(s) 465
CseI GACGC 1 cut(s) 109
Csp6I GTAC 1 cut(s) 376
CviAII CATG 2 cut(s) 325, 649
CviQI GTAC 1 cut(s) 376
DdeI CTNAG 4 cut(s) 30, 359, 533, 608
DpnI GATC 1 cut(s) 7
DpnII GATC 1 cut(s) 5
DraIII CACNNNGTG 1 cut(s) 264
EciI GGCGGA 4 cut(s) 131, 443, 506, 578
Eco57I CTGAAG 1 cut(s) 323
Eco88I CYCGRG 3 cut(s) 59, 189, 465
EcoNI CCTNNNNNAGG 1 cut(s) 155
EcoRII CCWGG 3 cut(s) 9, 201, 251
Esp3I CGTCTC 1 cut(s) 92
FaeI CATG 2 cut(s) 328, 652
FaiI YATR 2 cut(s) 326, 650
FatI CATG 2 cut(s) 324, 648
FokI GGATG 2 cut(s) 206, 451
FspBI CTAG 1 cut(s) 497
GsuI CTGGAG 1 cut(s) 53
HaeIII GGCC 2 cut(s) 206, 452
HapII CCGG 2 cut(s) 454, 466
HgaI GACGC 1 cut(s) 109
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 2 cut(s) 328, 652
HinfI GANTC 2 cut(s) 296, 610
HpaII CCGG 2 cut(s) 454, 466
HphI GGTGA 4 cut(s) 352, 520, 538, 562
Hpy188I TCNGA 3 cut(s) 31, 94, 331
Hpy188III TCNNGA 3 cut(s) 40, 191, 580
Hpy99I CGWCG 1 cut(s) 296
HpyAV CCTTC 6 cut(s) 161, 217, 340, 517, 550, 583
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 1 cut(s) 198
HpyCH4V TGCA 3 cut(s) 619, 648, 655
HpyF10VI GCNNNNNNNGC 9 cut(s) 64, 70, 73, 277, 286, 361, 458, 467, 502
HpyF3I CTNAG 4 cut(s) 30, 359, 533, 608
HpySE526I ACGT 1 cut(s) 198
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 2 cut(s) 328, 652
Kzo9I GATC 1 cut(s) 5
LmnI GCTCC 5 cut(s) 16, 72, 118, 334, 493
Lsp1109I GCAGC 7 cut(s) 76, 85, 169, 172, 367, 424, 625
LweI GCATC 1 cut(s) 519
MaeI CTAG 1 cut(s) 497
MaeII ACGT 1 cut(s) 198
MaeIII GTNAC 2 cut(s) 256, 688
MalI GATC 1 cut(s) 7
MboI GATC 1 cut(s) 5
MboII GAAGA 5 cut(s) 202, 316, 319, 379, 693
MflI RGATCY 1 cut(s) 5
MluCI AATT 1 cut(s) 446
MlyI GAGTC 2 cut(s) 305, 619
MmeI TCCRAC 4 cut(s) 72, 309, 522, 579
MseI TTAA 2 cut(s) 660, 712
MspA1I CMGCKG 1 cut(s) 358
MspI CCGG 2 cut(s) 454, 466
MspR9I CCNGG 6 cut(s) 11, 203, 253, 454, 466, 467
Mva1269I GAATGC 1 cut(s) 247
MvaI CCWGG 3 cut(s) 11, 203, 253
MvnI CGCG 1 cut(s) 103
MwoI GCNNNNNNNGC 9 cut(s) 64, 70, 73, 277, 286, 361, 458, 467, 502
NciI CCSGG 3 cut(s) 454, 466, 467
NdeII GATC 1 cut(s) 5
NlaIII CATG 2 cut(s) 328, 652
NmuCI GTSAC 2 cut(s) 256, 688
NspI RCATGY 1 cut(s) 652
PaeR7I CTCGAG 1 cut(s) 59
PaqCI CACCTGC 2 cut(s) 328, 406
PctI GAATGC 1 cut(s) 247
PleI GAGTC 2 cut(s) 304, 618
PpsI GAGTC 2 cut(s) 304, 618
Psp6I CCWGG 3 cut(s) 9, 201, 251
PspGI CCWGG 3 cut(s) 9, 201, 251
PspPI GGNCC 1 cut(s) 451
PspXI VCTCGAGB 1 cut(s) 59
PsuI RGATCY 1 cut(s) 5
PvuII CAGCTG 1 cut(s) 358
RsaI GTAC 1 cut(s) 377
RsaNI GTAC 1 cut(s) 376
SaqAI TTAA 2 cut(s) 660, 712
Sau3AI GATC 1 cut(s) 5
Sau96I GGNCC 1 cut(s) 451
SchI GAGTC 2 cut(s) 305, 619
ScrFI CCNGG 6 cut(s) 11, 203, 253, 454, 466, 467
SfaNI GCATC 1 cut(s) 519
Sfr274I CTCGAG 1 cut(s) 59
SlaI CTCGAG 1 cut(s) 59
SmaI CCCGGG 1 cut(s) 467
SmlI CTYRAG 3 cut(s) 59, 83, 578
SmoI CTYRAG 3 cut(s) 59, 83, 578
Sse9I AATT 1 cut(s) 446
SsiI CCGC 8 cut(s) 103, 116, 428, 485, 488, 491, 560, 563
SspMI CTAG 1 cut(s) 497
StyD4I CCNGG 6 cut(s) 9, 201, 251, 452, 464, 465
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 1 cut(s) 201
TaqI TCGA 4 cut(s) 39, 60, 137, 294
TasI AATT 1 cut(s) 446
TauI GCSGC 4 cut(s) 106, 488, 491, 563
Tru1I TTAA 2 cut(s) 660, 712
Tru9I TTAA 2 cut(s) 660, 712
TseFI GTSAC 2 cut(s) 256, 688
TseI GCWGC 7 cut(s) 64, 73, 182, 185, 355, 412, 638
Tsp45I GTSAC 2 cut(s) 256, 688
TspDTI ATGAA 2 cut(s) 536, 569
TspMI CCCGGG 1 cut(s) 465
XagI CCTNNNNNAGG 1 cut(s) 155
XceI RCATGY 1 cut(s) 652
XhoI CTCGAG 1 cut(s) 59
XmaI CCCGGG 1 cut(s) 465
XspI CTAG 1 cut(s) 497
ZraI GACGTC 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.