MD14G1136000.v1.1

Seed dormancy control

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015490
Physical Location & Seq
Reverse (-)
21608447 .. 21609180
734 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1136000.v1.1.491

Sequence Viewer

Length: 573 bp
ATGGTCTCCTTGGTCATAAGCTTGCAGGTGGAGCAGAGTTTCTCTGCATTTTTTGAGAAATGGGTTTTTCAGGTCGAGCAGCTCCAACATCAACTTCTCATGTTGTCGGAGGAGACACGGCAGAACGAGGCGGAGCTTTGGGCTTTGGTGTCGAAAACCACCAACCTTCACAAGGAGTATTACACCGTGAAGTGGGTTGCTGCTCGAGATGACGTCATGGCCTTCTTCTGCCCTGTTTGGTCAAGCCCTTTGGAGAATGCGTATTCCTGGTTCACCGGGCTGGTTTCCATGTCGAAAGAGCAGGTGAGGAAGGTTGAGCAGCTGCGGCAAAAGATGAGGTACAAGGAAGATAAGGTGGAGAGGGAAATGGATAGGCAGCAGGTGGCGATGGCGGACAAGAGGATGGTGGAATTGGCCTGGCTGGTGACCCGGGCGAGGAACAGAAAAGTGACGACGGAGCTGGAAGGGCTGGTGAATGTGGTGATGAAGGTGATGTTGAGTGGGTTGGAGGGGGTGATGAAGGCGGCATATTGTGAGAGGCTCAAGACATTGAAGAGTTGTTGGATTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DOG1

Protein Analysis

191

Amino Acids

22.39

Weight (kDa)

8.63

Isoelectric Point (pI)

38.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DOG1 PF14144 28 - 94 3.2e-14 Seed dormancy control
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015490)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18650
fragaria_vesca FvH4_3g07650
malus_domestica MD05G1073300.v1.1 MD05G1299100.v1.1 MD10G1044600.v1.1 MD14G1136000.v1.1
prunus_persica Prupe.4G062700_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0011031
rosa_laevigata RLG00000031804
rosa_multiflora Rmu_sc0003242.1_g000003
rosa_roxburghii Rroxscaffold_1G00065370
rosa_rugosa Rorug04G0454700
rosa_samantha Rh5BG081600 Rh5DG081400
rosa_wichuraiana Rw0G000130 Rw0G017820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 16, 292, 370
AatII GACGTC 1 cut(s) 216
Acc36I ACCTGC 3 cut(s) 16, 292, 370
AciI CCGC 4 cut(s) 131, 325, 392, 524
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 1 cut(s) 341
AfiI CCNNNNNNNGG 3 cut(s) 172, 192, 435
AgsI TTSAA 1 cut(s) 553
AjnI CCWGG 2 cut(s) 266, 416
AjuI GAANNNNNNNTTGG 2 cut(s) 78, 110
AluBI AGCT 5 cut(s) 21, 82, 136, 322, 460
AluI AGCT 5 cut(s) 21, 82, 136, 322, 460
Alw26I GTCTC 2 cut(s) 10, 107
Ama87I CYCGRG 2 cut(s) 204, 429
AoxI GGCC 2 cut(s) 219, 414
ApeKI GCWGC 5 cut(s) 79, 200, 319, 322, 376
AsuC2I CCSGG 3 cut(s) 277, 430, 431
AsuHPI GGTGA 7 cut(s) 265, 316, 436, 484, 493, 502, 526
AvaI CYCGRG 2 cut(s) 204, 429
BbvI GCAGC 5 cut(s) 91, 187, 309, 331, 388
BccI CCATC 2 cut(s) 382, 397
BceAI ACGGC 1 cut(s) 134
BciT130I CCWGG 2 cut(s) 268, 418
BcnI CCSGG 3 cut(s) 277, 430, 431
BcoDI GTCTC 2 cut(s) 10, 107
BfuAI ACCTGC 3 cut(s) 16, 292, 370
BisI GCNGC 7 cut(s) 80, 201, 320, 323, 326, 377, 525
BlsI GCNGC 7 cut(s) 81, 202, 321, 324, 327, 378, 526
Bme1390I CCNGG 5 cut(s) 268, 277, 418, 430, 431
BmeT110I CYCGRG 2 cut(s) 204, 429
BmrFI CCNGG 5 cut(s) 268, 277, 418, 430, 431
BpuEI CTTGAG 1 cut(s) 527
BpuMI CCSGG 3 cut(s) 277, 430, 431
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 1 cut(s) 10
BsaJI CCNNGG 2 cut(s) 9, 429
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 3 cut(s) 172, 192, 435
BseBI CCWGG 2 cut(s) 268, 418
BseDI CCNNGG 2 cut(s) 9, 429
BseGI GGATG 1 cut(s) 408
BseLI CCNNNNNNNGG 3 cut(s) 172, 192, 435
BseRI GAGGAG 1 cut(s) 125
BseXI GCAGC 5 cut(s) 91, 187, 309, 331, 388
BshFI GGCC 2 cut(s) 221, 416
BsiHKCI CYCGRG 2 cut(s) 204, 429
BsiSI CCGG 2 cut(s) 276, 430
BslI CCNNNNNNNGG 3 cut(s) 172, 192, 435
BsmAI GTCTC 2 cut(s) 10, 107
BsmI GAATGC 1 cut(s) 262
BsnI GGCC 2 cut(s) 221, 416
Bso31I GGTCTC 1 cut(s) 10
BsoBI CYCGRG 2 cut(s) 204, 429
BspACI CCGC 4 cut(s) 131, 325, 392, 524
BspANI GGCC 2 cut(s) 221, 416
BspMI ACCTGC 3 cut(s) 16, 292, 370
BspTNI GGTCTC 1 cut(s) 10
BssECI CCNNGG 2 cut(s) 9, 429
BssNI GRCGYC 1 cut(s) 213
BssT1I CCWWGG 1 cut(s) 9
Bst2UI CCWGG 2 cut(s) 268, 418
Bst4CI ACNGT 1 cut(s) 187
Bst6I CTCTTC 1 cut(s) 548
BstACI GRCGYC 1 cut(s) 213
BstC8I GCNNGC 1 cut(s) 23
BstEII GGTNACC 1 cut(s) 424
BstENI CCTNNNNNAGG 1 cut(s) 170
BstF5I GGATG 1 cut(s) 408
BstMAI GTCTC 2 cut(s) 10, 107
BstMWI GCNNNNNNNGC 3 cut(s) 31, 325, 466
BstNI CCWGG 2 cut(s) 268, 418
BstPI GGTNACC 1 cut(s) 424
BstSCI CCNGG 5 cut(s) 266, 275, 416, 428, 429
BstV1I GCAGC 5 cut(s) 91, 187, 309, 331, 388
BsuRI GGCC 2 cut(s) 221, 416
BtgZI GCGATG 1 cut(s) 401
BtsCI GGATG 1 cut(s) 408
BveI ACCTGC 3 cut(s) 16, 292, 370
Cac8I GCNNGC 1 cut(s) 23
Cfr9I CCCGGG 1 cut(s) 429
Csp6I GTAC 1 cut(s) 340
CviAII CATG 3 cut(s) 100, 217, 289
CviQI GTAC 1 cut(s) 340
Eam1104I CTCTTC 1 cut(s) 548
EarI CTCTTC 1 cut(s) 548
EciI GGCGGA 2 cut(s) 146, 407
Eco130I CCWWGG 1 cut(s) 9
Eco31I GGTCTC 1 cut(s) 10
Eco88I CYCGRG 2 cut(s) 204, 429
Eco91I GGTNACC 1 cut(s) 424
EcoNI CCTNNNNNAGG 1 cut(s) 170
EcoO65I GGTNACC 1 cut(s) 424
EcoRII CCWGG 2 cut(s) 266, 416
EcoT14I CCWWGG 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 9
FaeI CATG 3 cut(s) 103, 220, 292
FaiI YATR 5 cut(s) 17, 101, 218, 290, 529
FatI CATG 3 cut(s) 99, 216, 288
Fnu4HI GCNGC 7 cut(s) 80, 201, 320, 323, 326, 377, 525
FokI GGATG 1 cut(s) 415
Fsp4HI GCNGC 7 cut(s) 80, 201, 320, 323, 326, 377, 525
GluI GCNGC 7 cut(s) 80, 201, 320, 323, 326, 377, 525
HaeIII GGCC 2 cut(s) 221, 416
HapII CCGG 2 cut(s) 276, 430
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 3 cut(s) 103, 220, 292
HindIII AAGCTT 1 cut(s) 19
HpaII CCGG 2 cut(s) 276, 430
HphI GGTGA 7 cut(s) 265, 316, 436, 484, 493, 502, 526
Hpy166II GTNNAC 1 cut(s) 273
Hpy188I TCNGA 1 cut(s) 109
Hpy188III TCNNGA 2 cut(s) 206, 544
Hpy8I GTNNAC 1 cut(s) 273
Hpy99I CGWCG 1 cut(s) 457
HpyAV CCTTC 6 cut(s) 176, 232, 304, 458, 481, 514
HpyCH4III ACNGT 1 cut(s) 187
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 2 cut(s) 25, 47
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 325, 466
HpySE526I ACGT 1 cut(s) 213
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 3 cut(s) 103, 220, 292
LmnI GCTCC 4 cut(s) 31, 87, 133, 457
Lsp1109I GCAGC 5 cut(s) 91, 187, 309, 331, 388
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 2 cut(s) 424, 448
MboII GAAGA 3 cut(s) 217, 359, 565
MluCI AATT 1 cut(s) 410
MmeI TCCRAC 4 cut(s) 87, 109, 486, 542
MnlI CCTC 9 cut(s) 103, 121, 300, 330, 354, 393, 429, 502, 531
MspA1I CMGCKG 1 cut(s) 322
MspI CCGG 2 cut(s) 276, 430
MspR9I CCNGG 5 cut(s) 268, 277, 418, 430, 431
Mva1269I GAATGC 1 cut(s) 262
MvaI CCWGG 2 cut(s) 268, 418
MwoI GCNNNNNNNGC 3 cut(s) 31, 325, 466
NciI CCSGG 3 cut(s) 277, 430, 431
NlaIII CATG 3 cut(s) 103, 220, 292
NmuCI GTSAC 2 cut(s) 424, 448
PaeR7I CTCGAG 1 cut(s) 204
PaqCI CACCTGC 3 cut(s) 16, 292, 370
PctI GAATGC 1 cut(s) 262
PkrI GCNGC 7 cut(s) 81, 202, 321, 324, 327, 378, 526
Psp6I CCWGG 2 cut(s) 266, 416
PspEI GGTNACC 1 cut(s) 424
PspGI CCWGG 2 cut(s) 266, 416
PsrI GAACNNNNNNTAC 2 cut(s) 254, 286
PvuII CAGCTG 1 cut(s) 322
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
SatI GCNGC 7 cut(s) 80, 201, 320, 323, 326, 377, 525
ScrFI CCNGG 5 cut(s) 268, 277, 418, 430, 431
Sfr274I CTCGAG 1 cut(s) 204
SlaI CTCGAG 1 cut(s) 204
SmaI CCCGGG 1 cut(s) 431
SmlI CTYRAG 2 cut(s) 204, 542
SmoI CTYRAG 2 cut(s) 204, 542
Sse9I AATT 1 cut(s) 410
SsiI CCGC 4 cut(s) 131, 325, 392, 524
StyD4I CCNGG 5 cut(s) 266, 275, 416, 428, 429
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 1 cut(s) 187
TaiI ACGT 1 cut(s) 216
TaqI TCGA 4 cut(s) 75, 152, 205, 293
TasI AATT 1 cut(s) 410
TauI GCSGC 2 cut(s) 328, 527
TseFI GTSAC 2 cut(s) 424, 448
TseI GCWGC 5 cut(s) 79, 200, 319, 322, 376
Tsp45I GTSAC 2 cut(s) 424, 448
TspDTI ATGAA 2 cut(s) 500, 533
TspGWI ACGGA 1 cut(s) 470
TspMI CCCGGG 1 cut(s) 429
XagI CCTNNNNNAGG 1 cut(s) 170
XhoI CTCGAG 1 cut(s) 204
XmaI CCCGGG 1 cut(s) 429
ZraI GACGTC 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.