MD05G1309900.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012987
Physical Location & Seq
Reverse (-)
44048006 .. 44051708
3703 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1309900.v1.1.491

Sequence Viewer

Length: 3000 bp
ATGCTTCTCTTCCTCCCCCTCCTCCTCCTCCTCCTCCTGCCGCCACTCCCCACCACCCTCTCCCTCAACCAAGAAGGCCTCTACCTCCGACACTTCAAGCTCTCCCTCGATGACCCCGACTCCGCCCTCTCCTCCTGGAACTACGCCGACTCCACCCCCTGCAATTGGCTTGGCGTCACATGCGACGACGCCTCCTCCTCCTCCCCCGTCGTCCGCTCCCTCGACCTCCCCAGCGCCAACCTCGCAGGCCCTTTCCCCACCGTCCTCTGCCGCCTCCCCAACCTCACCCACCTCTCCCTCTACAACAACTCCATCAACTCCACCCTCCCTCCCTCCCTCTCCACGTGCCAAACTCTCGAGGACCTCGACCTCGCCCAGAACCTCCTCACCGGCGCCCTCCCCGCCACCCTCCCCGACCTCCCCAACCTCAAATACCTTGACCTCAGCGGAAATAATTTCTCCGGGGCAATTCCCGACTCCTTCGGCCGCTTCCAGAAACTCGAGGTCCTCTCTCTCGTCTACAATCTCATCGAAAACACCATCCCGCCCTTTCTTGGCAACATCTCCACCCTGAAAATGCTCAACCTCTCCTACAACCCGTTTCACCCGGGTCGGATCCCGGCGGAGCTCGGCAATTTGACGAACCTCGAGGTCCTCTGGCTCACCGAGTGCAACCTAGTCGGCGAGATTCCCGACTCGCTAGGTCGACTCAAAAACCTCAAGGATTTGGACCTCGCCATCAACGGCTTAACCGGCCGGATTCCGCCGTCGCTCAGCGAGCTGACCAGCGTCGTCCAGATTGAGCTCTACAACAACTCATTAACCGGCGAGCTGCCGCCTGGAATGTCGAAATTGACCCGGCTGAGACTCCTCGACGCGTCCATGAACCAGCTGAGCGGGCAGATTCCCGACGAGCTCTGCCGGTTGCCGCTCGAAAGCCTCAACCTTTACGAGAACAACTTAGAGGGGAGCGTGCCGGCGAGCATTGCGAACTCTCCCAACTTGTACGAGGTCAGGCTCTTCCGAAACAAGCTCTCCGGCGAGCTGCCCCAAAATCTCGGGAAGAACTCGCCGCTTAAGTGGTTTGATGTGTCGAGCAACCAGTTCACGGGCACCATTCCGGCGAGTCTGTGCGAGAAGGGACAAATGGAAGAGATTCTAATGCTACACAATGAGTTCTCCGGCGAGATCCCGGCAAGGTTGGGCGAGTGCCAGAGCTTGGCCCGGGTCCGATTGGGTCACAACAGGTTAAGTGGGGAAGTTCCGGTGGGCTTCTGGGGGCTGCCGCGCGTGTACTTGATGGAGCTCGCTGAGAATGAGCTTTCTGGGCCAATAGCGAAGAGCATTGCCAGAGCCACGAACCTCTCACTATTAATCCTAGCGAAGAACAAGTTTTCGGGCCCGATACCGGAGGAGATTGGGTGGGTGGAAAATCTTATGGAGTTTTCCGGTGGTGACAATAAGTTCAGTGGGCCTTTGCCGGAGAGCATTGTGAGACTTGGGCAGCTGGGGACTCTGGACCTTCACAGCAATGAAGTCTCCGGTGAGCTCCCAGTTGGGATCCAATCTTGGACGAAGCTAAACGAGCTCAATCTGGCAAGTAACCAACTTTCCGGGAAAATCCCAGATGGAATTGGGAACTTGTCCGTCCTTAATTACCTTGATCTTTCCGGGAATCGATTTTCCGGGAAAATCCCATTTGGGTTGCAGAATATGAAGCTCAATGTATTTAATTTGTCCTACAATCAGCTCTCAGGTGAGCTTCCTCCCCTTTTTGCTAAAGAAATATATAGGAATAGCTTCCTGGGGAATCCTGGTCTTTGTGGAGATTTGGATGGGTTGTGTGATAGCAGAGCCGAGGTTAAAAGTCAGGGGTACATATGGTTGCTTAGGTGCATGTTCATTCTGTCTGGTTTAGTATTTGTTGTGGGCGTGGTTTGGTTTTACTTGAAGTACAAGAACTTCAAGAAGGTGAACCGAACCATAGACAAATCAAAATGGACGCTGATGTCGTTTCACAAACTGGGTTTTAGTGAGTATGAGATCTTGGATTGCCTTGATGAAGATAATGTGATTGGTAGTGGGGCGTCGGGGAAGGTGTACAAGGTTGTGCTGAACAGTGGAGAGGTTGTTGCTGTAAAGAAGCTATGGAGAAGGAAGGTAAAGGAGTGTGAAGTAGAAGATGTTGAGAAGGGTTGGGTGCAAGACGATGGCTTTGAGGCGGAGGTAGATACTCTGGGGAAGATTAGGCACAAGAACATTGTGAAGCTCTGGTGCTGTTGCACTGCTAGGGACTGCAAGCTCTTGGTTTATGAGTATATGCAAAATGGTAGTCTCGGTGATTTGTTGCATAGCAGCAAAGGAGGGTTGCTGGATTGGCCTACAAGGTTTAAGATAGCTCTAGATGCAGCGGAGGGGCTTTCTTATCTGCACCATGATTGTGTTCCTGCGATTGTTCATAGAGATGTGAAATCAAACAACATATTGTTGGACGGGGATTTTGGAGCTCGAGTGGCGGATTTTGGAGTAGCCAAGGAGGTTGATGCAACTGGGAAAGGTCTTAAATCCATGTCGATCATTGCAGGTTCTTGTGGTTACATTGCTCCAGAATATGCGTACACTCTCAGAGTGAACGAGAAGAGCGATATATACAGTTTCGGTGTGGTTATTCTGGAGCTGGTGACCGGGAGGCTCCCAGTCGATCCAGAGTTCGGAGAAAAGGACTTGGTGAAGTGGGTCTGCACCACATTGGATCAGAAAGGGGTAGACAATGTGGTTGACCCAAAACTTGAGTCTTGTTACAAGGAAGAAGTATGCAAGGTCCTCAACATTGGCCTCCTCTGCACCAGCCCTCTCCCGATTAATCGTCCATCAATGAGACGAGTAGTGAAGTTGTTGCAAGAAGTTGGCACAGAGAAACATCCCCAGGCTGCTAAAAAGGAGGGGAAATTGACCCCCTATTACTACGAAGACACCTCAGATCATGGAAGCGTAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1000

Amino Acids

110.14

Weight (kDa)

5.34

Isoelectric Point (pI)

34.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 22 - 62 7.2e-14 Leucine rich repeat N-terminal domain
LRR_14 PF23598 66 - 196 2.6e-07 Leucine-rich repeat region
LRR_8 PF13855 93 - 153 7.3e-09 Leucine rich repeat
LRR_4 PF12799 118 - 158 4.7e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 141 - 200 6.8e-08 Leucine rich repeat
LRR_14 PF23598 184 - 307 1e-09 Leucine-rich repeat region
LRR_14 PF23598 492 - 557 7.6e-07 Leucine-rich repeat region
LRR_8 PF13855 503 - 561 3.2e-06 Leucine rich repeat
Pkinase PF00069 689 - 915 3.2e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 689 - 964 5.7e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012987)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28440
fragaria_vesca FvH4_3g08530 FvH4_3g08530
malus_domestica MD05G1309900.v1.1 MD10G1288500.v1.1
prunus_persica Prupe.4G053100_v2.0.a1
pyrus_communis pycom05g28630
rosa_chinensis RchiOBHm_Chr5g0009311
rosa_laevigata RLG00000031647
rosa_multiflora Rmu_sc0006233.1_g000007
rosa_roxburghii Rroxscaffold_1G00066780 Rroxscaffold_1G00067480
rosa_rugosa Rorug04G0441700
rosa_samantha Rh5AG072200 Rh5BG068700 Rh5CG081100 Rh5DG068900
rosa_wichuraiana Rw5G006750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2008
AbsI CCTCGAGG 1 cut(s) 647
Acc36I ACCTGC 1 cut(s) 2573
AccB1I GGYRCC 2 cut(s) 392, 1112
AccB7I CCANNNNNTGG 1 cut(s) 1219
AccBSI CCGCTC 3 cut(s) 216, 897, 931
AccI GTMKAC 3 cut(s) 519, 706, 2766
AccII CGCG 3 cut(s) 878, 1288, 1290
AclWI GGATC 7 cut(s) 610, 623, 1183, 1555, 1568, 2696, 2760
AcoI YGGCCR 2 cut(s) 484, 754
AcvI CACGTG 1 cut(s) 345
AcyI GRCGYC 4 cut(s) 174, 189, 393, 2087
AdeI CACNNNGTG 1 cut(s) 669
AfaI GTAC 6 cut(s) 1007, 1295, 1877, 1955, 2102, 2618
AfiI CCNNNNNNNGG 6 cut(s) 165, 554, 1108, 1219, 1408, 2711
AflII CTTAAG 1 cut(s) 1076
AflIII ACRYGT 1 cut(s) 876
AgsI TTSAA 3 cut(s) 97, 1951, 1966
AjnI CCWGG 5 cut(s) 134, 838, 1803, 1813, 2925
Alw21I GWGCWC 7 cut(s) 630, 807, 918, 1308, 1551, 1590, 2509
Alw26I GTCTC 5 cut(s) 859, 1489, 1543, 2339, 2872
AlwI GGATC 7 cut(s) 610, 623, 1183, 1555, 1568, 2696, 2760
Ama87I CYCGRG 7 cut(s) 356, 500, 607, 647, 1058, 1224, 2508
ApaI GGGCCC 1 cut(s) 1403
ApeKI GCWGC 7 cut(s) 832, 1045, 1282, 1504, 2355, 2408, 2930
ArsI GACNNNNNNTTYG 8 cut(s) 2198, 2230, 2317, 2349, 2483, 2515, 2777, 2809
AseI ATTAAT 2 cut(s) 1373, 2862
Asp700I GAANNNNTTC 2 cut(s) 1155, 1799
AspLEI GCGC 3 cut(s) 236, 395, 1290
AvaI CYCGRG 7 cut(s) 356, 500, 607, 647, 1058, 1224, 2508
AvaII GGWCC 7 cut(s) 361, 505, 652, 730, 1228, 1519, 2821
BaeGI GKGCMC 2 cut(s) 1115, 1403
BamHI GGATCC 2 cut(s) 615, 1560
BanI GGYRCC 2 cut(s) 392, 1112
BanII GRGCYC 8 cut(s) 630, 807, 918, 1308, 1403, 1551, 1590, 2509
BarI GAAGNNNNNNTAC 2 cut(s) 1946, 1978
BbrPI CACGTG 1 cut(s) 345
BbsI GAAGAC 1 cut(s) 2976
Bbv12I GWGCWC 7 cut(s) 630, 807, 918, 1308, 1551, 1590, 2509
BbvCI CCTCAGC 1 cut(s) 443
BbvI GCAGC 7 cut(s) 819, 1032, 1269, 1516, 2367, 2420, 2917
BccI CCATC 8 cut(s) 320, 548, 746, 1294, 1622, 1829, 2204, 2878
BceAI ACGGC 2 cut(s) 751, 760
BcgI CGANNNNNNTGC 2 cut(s) 661, 695
BciT130I CCWGG 5 cut(s) 136, 840, 1805, 1815, 2927
BcoDI GTCTC 5 cut(s) 859, 1489, 1543, 2339, 2872
BfaI CTAG 5 cut(s) 677, 701, 1379, 2289, 2402
BfoI RGCGCY 2 cut(s) 237, 396
BfrI CTTAAG 1 cut(s) 1076
BfuAI ACCTGC 1 cut(s) 2573
BglII AGATCT 1 cut(s) 2043
BlpI GCTNAGC 2 cut(s) 773, 893
Bme18I GGWCC 7 cut(s) 361, 505, 652, 730, 1228, 1519, 2821
BmeT110I CYCGRG 7 cut(s) 356, 500, 607, 647, 1058, 1224, 2508
BmiI GGNNCC 7 cut(s) 394, 617, 1114, 1229, 1401, 1562, 2693
BmrI ACTGGG 4 cut(s) 1547, 2033, 2559, 2690
BmsI GCATC 2 cut(s) 2395, 2533
BmuI ACTGGG 4 cut(s) 1547, 2033, 2559, 2690
BoxI GACNNNNGTC 1 cut(s) 788
BpiI GAAGAC 1 cut(s) 2976
BplI GAGNNNNNCTC 2 cut(s) 494, 526
BpmI CTGGAG 2 cut(s) 2589, 2693
Bpu10I CCTNAGC 2 cut(s) 443, 1889
Bpu1102I GCTNAGC 2 cut(s) 773, 893
BpuEI CTTGAG 2 cut(s) 704, 2810
Bsa29I ATCGAT 1 cut(s) 1678
BsaAI YACGTR 1 cut(s) 345
BsaHI GRCGYC 4 cut(s) 174, 189, 393, 2087
BsaJI CCNNGG 7 cut(s) 462, 607, 1224, 1804, 1857, 2532, 2925
BsaWI WCCGGW 4 cut(s) 1264, 1408, 1448, 1541
Bsc4I CCNNNNNNNGG 6 cut(s) 165, 554, 1108, 1219, 1408, 2711
Bse118I RCCGGY 5 cut(s) 389, 752, 824, 921, 976
Bse1I ACTGG 5 cut(s) 1102, 1553, 2028, 2554, 2696
Bse3DI GCAATG 5 cut(s) 984, 1344, 1537, 2577, 2598
BseBI CCWGG 5 cut(s) 136, 840, 1805, 1815, 2927
BseCI ATCGAT 1 cut(s) 1678
BseDI CCNNGG 7 cut(s) 462, 607, 1224, 1804, 1857, 2532, 2925
BseGI GGATG 3 cut(s) 540, 1840, 2920
BseLI CCNNNNNNNGG 6 cut(s) 165, 554, 1108, 1219, 1408, 2711
BseMI GCAATG 5 cut(s) 984, 1344, 1537, 2577, 2598
BseMII CTCAG 8 cut(s) 457, 787, 854, 884, 1302, 1767, 2638, 2991
BseNI ACTGG 5 cut(s) 1102, 1553, 2028, 2554, 2696
BseSI GKGCMC 2 cut(s) 1115, 1403
BseX3I CGGCCG 2 cut(s) 484, 754
BseXI GCAGC 7 cut(s) 819, 1032, 1269, 1516, 2367, 2420, 2917
BseYI CCCAGC 2 cut(s) 230, 1507
BsgI GTGCAG 3 cut(s) 2414, 2725, 2827
Bsh1236I CGCG 3 cut(s) 878, 1288, 1290
Bsh1285I CGRYCG 2 cut(s) 487, 757
BshNI GGYRCC 2 cut(s) 392, 1112
BshVI ATCGAT 1 cut(s) 1678
BsiEI CGRYCG 2 cut(s) 487, 757
BsiHKAI GWGCWC 7 cut(s) 630, 807, 918, 1308, 1551, 1590, 2509
BsiHKCI CYCGRG 7 cut(s) 356, 500, 607, 647, 1058, 1224, 2508
BslFI GGGAC 3 cut(s) 1155, 1525, 2306
BslI CCNNNNNNNGG 6 cut(s) 165, 554, 1108, 1219, 1408, 2711
BsmAI GTCTC 5 cut(s) 859, 1489, 1543, 2339, 2872
BsmBI CGTCTC 1 cut(s) 2872
BsmFI GGGAC 3 cut(s) 1155, 1525, 2306
BsoBI CYCGRG 7 cut(s) 356, 500, 607, 647, 1058, 1224, 2508
Bsp120I GGGCCC 1 cut(s) 1399
Bsp1286I GDGCHC 9 cut(s) 630, 807, 918, 1115, 1308, 1403, 1551, 1590, 2509
Bsp1407I TGTACA 1 cut(s) 2100
Bsp143I GATC 9 cut(s) 615, 1188, 1560, 1663, 2043, 2574, 2701, 2752, 2980
Bsp1720I GCTNAGC 2 cut(s) 773, 893
BspCNI CTCAG 8 cut(s) 456, 786, 855, 885, 1303, 1766, 2637, 2990
BspDI ATCGAT 1 cut(s) 1678
BspFNI CGCG 3 cut(s) 878, 1288, 1290
BspLI GGNNCC 7 cut(s) 394, 617, 1114, 1229, 1401, 1562, 2693
BspMI ACCTGC 1 cut(s) 2573
BspPI GGATC 7 cut(s) 610, 623, 1183, 1555, 1568, 2696, 2760
BspQI GCTCTTC 3 cut(s) 1025, 1334, 2633
BspT107I GGYRCC 2 cut(s) 392, 1112
BspTI CTTAAG 1 cut(s) 1076
BsrBI CCGCTC 3 cut(s) 216, 897, 931
BsrDI GCAATG 5 cut(s) 984, 1344, 1537, 2577, 2598
BsrFI RCCGGY 5 cut(s) 389, 752, 824, 921, 976
BsrGI TGTACA 1 cut(s) 2100
BsrI ACTGG 5 cut(s) 1102, 1553, 2028, 2554, 2696
BssAI RCCGGY 5 cut(s) 389, 752, 824, 921, 976
BssECI CCNNGG 7 cut(s) 462, 607, 1224, 1804, 1857, 2532, 2925
BssMI GATC 9 cut(s) 615, 1188, 1560, 1663, 2043, 2574, 2701, 2752, 2980
BssNI GRCGYC 4 cut(s) 174, 189, 393, 2087
BssT1I CCWWGG 1 cut(s) 2532
Bst2UI CCWGG 5 cut(s) 136, 840, 1805, 1815, 2927
Bst4CI ACNGT 3 cut(s) 262, 2120, 2654
Bst6I CTCTTC 5 cut(s) 14, 1025, 1146, 1334, 2633
BstACI GRCGYC 4 cut(s) 174, 189, 393, 2087
BstAFI CTTAAG 1 cut(s) 1076
BstAUI TGTACA 1 cut(s) 2100
BstBAI YACGTR 1 cut(s) 345
BstEII GGTNACC 1 cut(s) 2680
BstF5I GGATG 3 cut(s) 540, 1840, 2920
BstFNI CGCG 3 cut(s) 878, 1288, 1290
BstH2I RGCGCY 2 cut(s) 237, 396
BstHHI GCGC 3 cut(s) 236, 395, 1290
BstKTI GATC 9 cut(s) 618, 1191, 1563, 1666, 2046, 2577, 2704, 2755, 2983
BstMAI GTCTC 5 cut(s) 859, 1489, 1543, 2339, 2872
BstMBI GATC 9 cut(s) 615, 1188, 1560, 1663, 2043, 2574, 2701, 2752, 2980
BstMCI CGRYCG 2 cut(s) 487, 757
BstNI CCWGG 5 cut(s) 136, 840, 1805, 1815, 2927
BstNSI RCATGY 2 cut(s) 183, 1900
BstPAI GACNNNNGTC 1 cut(s) 788
BstPI GGTNACC 1 cut(s) 2680
BstSLI GKGCMC 2 cut(s) 1115, 1403
BstUI CGCG 3 cut(s) 878, 1288, 1290
BstV1I GCAGC 7 cut(s) 819, 1032, 1269, 1516, 2367, 2420, 2917
BstV2I GAAGAC 1 cut(s) 2976
BstX2I RGATCY 4 cut(s) 615, 1188, 1560, 2043
BstYI RGATCY 4 cut(s) 615, 1188, 1560, 2043
BstZI CGGCCG 2 cut(s) 484, 754
Bsu15I ATCGAT 1 cut(s) 1678
BsuTUI ATCGAT 1 cut(s) 1678
BtsCI GGATG 3 cut(s) 540, 1840, 2920
BtsI GCAGTG 1 cut(s) 2283
BtsIMutI CAGTG 3 cut(s) 1474, 2125, 2283
BveI ACCTGC 1 cut(s) 2573
CfoI GCGC 3 cut(s) 236, 395, 1290
Cfr10I RCCGGY 5 cut(s) 389, 752, 824, 921, 976
Cfr9I CCCGGG 2 cut(s) 607, 1224
ClaI ATCGAT 1 cut(s) 1678
CseI GACGC 7 cut(s) 163, 197, 778, 867, 884, 2011, 2076
Csp6I GTAC 6 cut(s) 1006, 1294, 1876, 1954, 2101, 2617
CviAII CATG 6 cut(s) 180, 883, 1897, 2435, 2569, 2984
CviQI GTAC 6 cut(s) 1006, 1294, 1876, 1954, 2101, 2617
DinI GGCGCC 1 cut(s) 394
DpnI GATC 9 cut(s) 617, 1190, 1562, 1665, 2045, 2576, 2703, 2754, 2982
DpnII GATC 9 cut(s) 615, 1188, 1560, 1663, 2043, 2574, 2701, 2752, 2980
DraIII CACNNNGTG 1 cut(s) 669
DrdI GACNNNNNNGTC 1 cut(s) 2008
DseDI GACNNNNNNGTC 1 cut(s) 2008
EaeI YGGCCR 2 cut(s) 484, 754
EagI CGGCCG 2 cut(s) 484, 754
Eam1104I CTCTTC 5 cut(s) 14, 1025, 1146, 1334, 2633
EarI CTCTTC 5 cut(s) 14, 1025, 1146, 1334, 2633
EciI GGCGGA 5 cut(s) 112, 638, 753, 2237, 2531
Ecl136II GAGCTC 7 cut(s) 628, 805, 916, 1306, 1549, 1588, 2507
EclXI CGGCCG 2 cut(s) 484, 754
Eco130I CCWWGG 1 cut(s) 2532
Eco147I AGGCCT 1 cut(s) 78
Eco24I GRGCYC 8 cut(s) 630, 807, 918, 1308, 1403, 1551, 1590, 2509
Eco47I GGWCC 7 cut(s) 361, 505, 652, 730, 1228, 1519, 2821
Eco52I CGGCCG 2 cut(s) 484, 754
Eco53kI GAGCTC 7 cut(s) 628, 805, 916, 1306, 1549, 1588, 2507
Eco72I CACGTG 1 cut(s) 345
Eco88I CYCGRG 7 cut(s) 356, 500, 607, 647, 1058, 1224, 2508
Eco91I GGTNACC 1 cut(s) 2680
EcoICRI GAGCTC 7 cut(s) 628, 805, 916, 1306, 1549, 1588, 2507
EcoO109I RGGNCCY 5 cut(s) 248, 361, 505, 652, 2821
EcoO65I GGTNACC 1 cut(s) 2680
EcoRII CCWGG 5 cut(s) 134, 838, 1803, 1813, 2925
EcoT14I CCWWGG 1 cut(s) 2532
EcoT38I GRGCYC 8 cut(s) 630, 807, 918, 1308, 1403, 1551, 1590, 2509
EgeI GGCGCC 1 cut(s) 394
EheI GGCGCC 1 cut(s) 394
ErhI CCWWGG 1 cut(s) 2532
Esp3I CGTCTC 1 cut(s) 2872
FaeI CATG 6 cut(s) 183, 886, 1900, 2438, 2572, 2987
FalI AAGNNNNNCTT 1 cut(s) 2980
FaqI GGGAC 3 cut(s) 1155, 1525, 2306
FatI CATG 6 cut(s) 179, 882, 1896, 2434, 2568, 2983
FauI CCCGC 3 cut(s) 409, 552, 890
FauNDI CATATG 1 cut(s) 1880
FblI GTMKAC 3 cut(s) 519, 706, 2766
FokI GGATG 3 cut(s) 527, 1847, 2907
FriOI GRGCYC 8 cut(s) 630, 807, 918, 1308, 1403, 1551, 1590, 2509
FspBI CTAG 5 cut(s) 677, 701, 1379, 2289, 2402
GlaI GCGC 3 cut(s) 235, 394, 1289
GsaI CCCAGC 2 cut(s) 234, 1511
GsuI CTGGAG 2 cut(s) 2589, 2693
HaeII RGCGCY 2 cut(s) 237, 396
HgaI GACGC 7 cut(s) 163, 197, 778, 867, 884, 2011, 2076
HhaI GCGC 3 cut(s) 236, 395, 1290
Hin1I GRCGYC 4 cut(s) 174, 189, 393, 2087
Hin1II CATG 6 cut(s) 183, 886, 1900, 2438, 2572, 2987
Hin6I GCGC 3 cut(s) 234, 393, 1288
HinP1I GCGC 3 cut(s) 234, 393, 1288
HincII GTYRAC 2 cut(s) 707, 2779
HindII GTYRAC 2 cut(s) 707, 2779
Hpy188I TCNGA 8 cut(s) 89, 615, 1025, 1232, 2627, 2714, 2757, 2980
Hpy99I CGWCG 8 cut(s) 188, 191, 212, 772, 794, 878, 914, 2092
HpyAV CCTTC 9 cut(s) 68, 490, 1132, 1532, 1963, 2089, 2148, 2152, 2185
HpyCH4III ACNGT 3 cut(s) 262, 2120, 2654
HpyCH4IV ACGT 1 cut(s) 344
HpySE526I ACGT 1 cut(s) 344
Hsp92I GRCGYC 4 cut(s) 174, 189, 393, 2087
Hsp92II CATG 6 cut(s) 183, 886, 1900, 2438, 2572, 2987
HspAI GCGC 3 cut(s) 234, 393, 1288
KasI GGCGCC 1 cut(s) 392
KroI GCCGGC 1 cut(s) 976
KroNI GCCGGC 1 cut(s) 978
Kzo9I GATC 9 cut(s) 615, 1188, 1560, 1663, 2043, 2574, 2701, 2752, 2980
LguI GCTCTTC 3 cut(s) 1025, 1334, 2633
LmnI GCTCC 9 cut(s) 221, 625, 969, 1303, 1554, 2504, 2608, 2674, 2697
Lsp1109I GCAGC 7 cut(s) 819, 1032, 1269, 1516, 2367, 2420, 2917
LweI GCATC 2 cut(s) 2395, 2533
MaeI CTAG 5 cut(s) 677, 701, 1379, 2289, 2402
MaeII ACGT 1 cut(s) 344
MaeIII GTNAC 7 cut(s) 175, 1238, 1454, 1601, 2594, 2680, 2798
MalI GATC 9 cut(s) 617, 1190, 1562, 1665, 2045, 2576, 2703, 2754, 2982
MbiI CCGCTC 3 cut(s) 216, 897, 931
MboI GATC 9 cut(s) 615, 1188, 1560, 1663, 2043, 2574, 2701, 2752, 2980
MfeI CAATTG 1 cut(s) 163
MflI RGATCY 4 cut(s) 615, 1188, 1560, 2043
MhlI GDGCHC 9 cut(s) 630, 807, 918, 1115, 1308, 1403, 1551, 1590, 2509
MluCI AATT 9 cut(s) 163, 454, 468, 634, 851, 1632, 1654, 1732, 2948
MluI ACGCGT 1 cut(s) 876
Mly113I GGCGCC 1 cut(s) 393
MlyI GAGTC 9 cut(s) 113, 143, 470, 689, 702, 861, 1135, 1507, 2801
MmeI TCCRAC 3 cut(s) 112, 593, 2469
MroNI GCCGGC 1 cut(s) 976
MroXI GAANNNNTTC 2 cut(s) 1155, 1799
MslI CAYNNNNRTG 3 cut(s) 1530, 2439, 2463
MspA1I CMGCKG 4 cut(s) 447, 892, 1507, 2411
MspCI CTTAAG 1 cut(s) 1076
MunI CAATTG 1 cut(s) 163
MvaI CCWGG 5 cut(s) 136, 840, 1805, 1815, 2927
MvnI CGCG 3 cut(s) 878, 1288, 1290
NaeI GCCGGC 1 cut(s) 978
NarI GGCGCC 1 cut(s) 393
NdeI CATATG 1 cut(s) 1880
NdeII GATC 9 cut(s) 615, 1188, 1560, 1663, 2043, 2574, 2701, 2752, 2980
NgoMIV GCCGGC 1 cut(s) 976
NlaIII CATG 6 cut(s) 183, 886, 1900, 2438, 2572, 2987
NlaIV GGNNCC 7 cut(s) 394, 617, 1114, 1229, 1401, 1562, 2693
NmeAIII GCCGAG 2 cut(s) 609, 1882
NmuCI GTSAC 4 cut(s) 175, 1238, 1454, 2680
NspI RCATGY 2 cut(s) 183, 1900
PaeR7I CTCGAG 4 cut(s) 356, 500, 647, 2508
PceI AGGCCT 1 cut(s) 78
PciSI GCTCTTC 3 cut(s) 1025, 1334, 2633
PcsI WCGNNNNNNNCGW 4 cut(s) 114, 363, 2009, 2640
PdiI GCCGGC 1 cut(s) 978
PdmI GAANNNNTTC 2 cut(s) 1155, 1799
PfeI GAWTC 6 cut(s) 688, 760, 904, 1156, 1675, 1810
PflMI CCANNNNNTGG 1 cut(s) 1219
PfoI TCCNGGA 4 cut(s) 134, 1613, 1670, 1685
PleI GAGTC 9 cut(s) 113, 143, 470, 689, 702, 861, 1134, 1507, 2800
PluTI GGCGCC 1 cut(s) 396
PmaCI CACGTG 1 cut(s) 345
PmlI CACGTG 1 cut(s) 345
PpsI GAGTC 9 cut(s) 113, 143, 470, 689, 702, 861, 1134, 1507, 2800
Ppu21I YACGTR 1 cut(s) 345
PpuMI RGGWCCY 4 cut(s) 361, 505, 652, 2821
PshAI GACNNNNGTC 1 cut(s) 788
PshBI ATTAAT 2 cut(s) 1373, 2862
Psp124BI GAGCTC 7 cut(s) 630, 807, 918, 1308, 1551, 1590, 2509
Psp5II RGGWCCY 4 cut(s) 361, 505, 652, 2821
Psp6I CCWGG 5 cut(s) 134, 838, 1803, 1813, 2925
PspCI CACGTG 1 cut(s) 345
PspEI GGTNACC 1 cut(s) 2680
PspFI CCCAGC 2 cut(s) 230, 1507
PspGI CCWGG 5 cut(s) 134, 838, 1803, 1813, 2925
PspN4I GGNNCC 7 cut(s) 394, 617, 1114, 1229, 1401, 1562, 2693
PspOMI GGGCCC 1 cut(s) 1399
PspPPI RGGWCCY 4 cut(s) 361, 505, 652, 2821
PspXI VCTCGAGB 3 cut(s) 500, 647, 2508
PsuI RGATCY 4 cut(s) 615, 1188, 1560, 2043
PvuII CAGCTG 2 cut(s) 892, 1507
RsaI GTAC 6 cut(s) 1007, 1295, 1877, 1955, 2102, 2618
RsaNI GTAC 6 cut(s) 1006, 1294, 1876, 1954, 2101, 2617
RseI CAYNNNNRTG 3 cut(s) 1530, 2439, 2463
SacI GAGCTC 7 cut(s) 630, 807, 918, 1308, 1551, 1590, 2509
SalI GTCGAC 1 cut(s) 705
SapI GCTCTTC 3 cut(s) 1025, 1334, 2633
Sau3AI GATC 9 cut(s) 615, 1188, 1560, 1663, 2043, 2574, 2701, 2752, 2980
SchI GAGTC 9 cut(s) 113, 143, 470, 689, 702, 861, 1135, 1507, 2801
SduI GDGCHC 9 cut(s) 630, 807, 918, 1115, 1308, 1403, 1551, 1590, 2509
SfaNI GCATC 2 cut(s) 2395, 2533
SfoI GGCGCC 1 cut(s) 394
Sfr274I CTCGAG 4 cut(s) 356, 500, 647, 2508
SgrAI CRCCGGYG 1 cut(s) 389
SinI GGWCC 7 cut(s) 361, 505, 652, 730, 1228, 1519, 2821
SlaI CTCGAG 4 cut(s) 356, 500, 647, 2508
SmaI CCCGGG 2 cut(s) 609, 1226
SmiMI CAYNNNNRTG 3 cut(s) 1530, 2439, 2463
SmlI CTYRAG 7 cut(s) 356, 500, 647, 719, 1076, 2508, 2789
SmoI CTYRAG 7 cut(s) 356, 500, 647, 719, 1076, 2508, 2789
Sse9I AATT 9 cut(s) 163, 454, 468, 634, 851, 1632, 1654, 1732, 2948
SseBI AGGCCT 1 cut(s) 78
SspDI GGCGCC 1 cut(s) 392
SspMI CTAG 5 cut(s) 677, 701, 1379, 2289, 2402
SstI GAGCTC 7 cut(s) 630, 807, 918, 1308, 1551, 1590, 2509
StuI AGGCCT 1 cut(s) 78
StyI CCWWGG 1 cut(s) 2532
TaaI ACNGT 3 cut(s) 262, 2120, 2654
TaiI ACGT 1 cut(s) 347
TasI AATT 9 cut(s) 163, 454, 468, 634, 851, 1632, 1654, 1732, 2948
TatI WGTACW 3 cut(s) 1293, 1953, 2100
TauI GCSGC 7 cut(s) 43, 273, 489, 838, 931, 1075, 1288
TfiI GAWTC 6 cut(s) 688, 760, 904, 1156, 1675, 1810
TscAI CASTG 3 cut(s) 1474, 2125, 2290
TseFI GTSAC 4 cut(s) 175, 1238, 1454, 2680
TseI GCWGC 7 cut(s) 832, 1045, 1282, 1504, 2355, 2408, 2930
Tsp45I GTSAC 4 cut(s) 175, 1238, 1454, 2680
TspDTI ATGAA 6 cut(s) 899, 1548, 1730, 1891, 2076, 2447
TspGWI ACGGA 1 cut(s) 1636
TspMI CCCGGG 2 cut(s) 607, 1224
TspRI CASTG 3 cut(s) 1474, 2125, 2290
Van91I CCANNNNNTGG 1 cut(s) 1219
Vha464I CTTAAG 1 cut(s) 1076
VpaK11BI GGWCC 7 cut(s) 361, 505, 652, 730, 1228, 1519, 2821
VspI ATTAAT 2 cut(s) 1373, 2862
XbaI TCTAGA 1 cut(s) 2401
XceI RCATGY 2 cut(s) 183, 1900
XhoI CTCGAG 4 cut(s) 356, 500, 647, 2508
XmaI CCCGGG 2 cut(s) 607, 1224
XmiI GTMKAC 3 cut(s) 519, 706, 2766
XmnI GAANNNNTTC 2 cut(s) 1155, 1799
XspI CTAG 5 cut(s) 677, 701, 1379, 2289, 2402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.