MD10G1288500.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0012987
Physical Location & Seq
Reverse (-)
37771705 .. 37775310
3606 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1288500.v1.1.491

Sequence Viewer

Length: 2997 bp
ATGCTTCTCTTCCTCCTCCCCCTCCTCCTCCTCCTCCCACCGCTCCCCACCACCCTCTCCCTCAACCAAGAAGGCCTCTACCTCCAACACTTCAAGCTCTCCCTCGACGATCCTGACTCCGCCCTCGACTCCTGGAACGACGCCGACTCCACCCCCTGCAATTGGCTTGGGGTCAAATGCGACGACGCCTCCTCCTCCTCCCCCGTCGTCCGCTCCCTCGACCTCCCCAGCGCCAACCTCGCCGGCCCTTTCCCCACCGTCCTCTGCCGCCTCCCCAACCTCACCCACCTCTCCCTCTACAACAACTCCATCAACTCCACCCTCCCTCCCTCACTCTCCACTTGTCAAAATCTCGAGCACCTTGACCTCTCCCAGAACCTTCTCACCGGCGCCCTCCCCGCCACCCTCCCTGACCTCCCCAACCTCAAATACCTCGACCTCACCGGAAACAACTTCTCCGGACCAATTCCCGACTCCTTCGGCCGCTTCCAGAAACTCGAGGTCCTCTCTCTCGTCTACAATCTCATTGAAGGCACCATACCCCCGTTTCTCGGCAACATTTCCACCCTGAAAATGCTCAACCTTTCCTACAACCCGTTTCTCCCGGGTCGGATCCCGGCGGAGCTCGGCAATCTGACGAACCTCGAGGTGCTCTGGCTCACCGAGTGCAACATAGTTGGCGAGATTCCCGACTCGCTGGGTCGTCTCAAAAACCTCAAGGATTTGGACCTCGCCATCAACGGCTTAACCGGCCGGATTCCGCCGTCGCTCAGCGAGCTGACCAGCGTCGTTCAGATTGAGCTCTACAACAACTCATTAACCGGCAAGCTGCCGCCCGGAATGTCTAAATTGACCCGGCTGAGACTCCTCGACGCGTCCATGAACCAGCTGAGCGGGCCAATTCCTGACGAGCTCTGCCGGTTGCCGCTCGAAAGCCTCAACCTTTACGAGAACAACTTCGAGGGGAGCGTGCCGGCGAGCATTGCGAACTCGCCTAACTTGTACGAGCTCAGGCTCTTCCGAAACAAGCTCTCCGGCGAGCTTCCCCAAAATCTCGGGAAGAACTCCCCTCTCAAGTGGCTCGACGTGTCTAGCAACCAGTTCACTGGCACCATTCCGGCGAGTCTTTGCGAGAAGCGACAAATGGAAGAGCTCTTGATGATACACAATGAGTTTTCCGGTGGGATCCCGGTGAGGTTGGGTGAGTGCCAGAGCTTGACCCGGGTTCGATTGGGTCACAACCGGTTAAGTGGGGAAGTTCCGGCGGGCTTCTGGGGGCTGCCGCGCGTGTACTTGATGGAGCTCGTTGAGAATGAGCTTTCTGGGGCGATATCGAAGACCATTGCCGGAGCCACGAACCTCTCGCTATTAATCGTAGCGAAGAACAAGTTTTCGGGTCAGATACCGGAGGAGATTGGGTGGGTGGAGAATCTTATGGAGTTTTCCGGTGGCGAAAATAAGTTCAATGGGCCGTTGCCGGAGAGCATTGTGAGACTCGGGCAGCTCGGGACTCTGGACCTCCACAGTAACGAAATCTCGGGTGAGCTTCCAATTGGGATCCAGTCTTGGACGAAACTCAACGAGCTCAATCTAGCAAGTAACCAACTTTCCGGGAAAATCCCAGATGGGATTGGGAACTTATCCGTGCTTAATTACCTTGATCTTTCCGGGAATCGATTTTCCGGGAAAATCCCATTTGGGTTGCAGAATATGAAGCTCAATGTGTTTAATTTGTCCAACAATCGGCTCTCAGGTGAGCTTCCACCCTTATTTGCTAAGGAAATTTATAGGAGTAGCTTTCTGGGAAATCCTGGTCTTTGTGGAGATTTGGATGGCTTATGTGATGGCAAAGCTGAGGTTAAAAGTCAGGGGTACCTATGGTTGCTTAGGTGCATCTTCATTCTATCTGGTTTAGTATTTGTTGTGGGGGTGGTCTGGTTTTACTTGAAGTACAAGAACTTCAAGAAGGCGAACCGAACGATAGACAAATCCAAATGGACGCTGATGTCGTTTCACAAACTGGGTTTTAGTGAGTATGAGATCTTGGATTGCCTTGATGAAGATAATGTGATTGGTAGCGGGGCGTCCGGGAAGGTGTACAAGGTTATGCTGAGCAGTGGAGAGGTTGTTGCAGTGAAGAAGCTTTGGGGAGGGAAAGTGCAGGAGTGCGAAGCTGGCGATGTTGAGAAAGGTTGGGTCCAAGACGACGGCTTTGAGGCGGAGGTGGAGACTTTGGGGAGGATTAGGCACAAGAACATTGTGAAGCTTTGGTGTTGTTGCACTACTAGGGACTGCAAGCTCTTGGTTTATGAGTATATGCAAAATGGCAGTCTCGGTGATATGTTGCATAGCATCAAAGGAGGGTTGCTGGATTGGCCTACAAGGTTTAAGATAGCTCTGGATGCAGCAGAGGGTCTTTCTTATCTGCACCATGATTGCGTTCCTGCGATTGTTCATAGAGATGTGAAATCCAACAACATATTGTTGGACGGCGATTTTGGAGCGCGAGTGGCGGATTTTGGAGTAGCCAAGGTGGTTGATGTGACTGGTAAAGGGCCTCAATCCATGTCGGGCATTACAGGTTCTTGTGGTTACATTGCTCCAGAATATGCATACACTCTCAGAGTGAATGAGAAGAGCGATATATACAGTTTCGGCGTGGTTATTCTGGAGCTGGTGACCGGGAGACTCCCAGTCGACCCAGAGTTCGGAGAAAAGGACTTGGTGAAGTGGGTCTGCACCGCATTGGATCAGAAAGGAGTAGACAGTGTGGTTGACCCAAAACTTGAATCTTGTTACAAGGAAGAAGTCGGCAAGGTCCTCAACATTGGCCTCCTCTGCACCAGCCCTCTCCCAATTAATCGTCCGTCCATGAGACGAGTAGTGAAATTGTTGCAAGAAGTTGGCACAGAGAAACATCCCCAGGCTGCTAAAAAGGAGGGGAAATTGTCACCCTATTACTACGAAGATGCCTCGGATCATGGAAGCGTAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

999

Amino Acids

109.56

Weight (kDa)

5.37

Isoelectric Point (pI)

35.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 21 - 61 7.9e-13 Leucine rich repeat N-terminal domain
LRR_14 PF23598 65 - 174 7.9e-07 Leucine-rich repeat region
LRR_8 PF13855 92 - 152 9e-10 Leucine rich repeat
LRR_4 PF12799 116 - 157 2.3e-07 Leucine Rich repeats (2 copies)
LRR_8 PF13855 140 - 199 2.1e-07 Leucine rich repeat
LRR_14 PF23598 184 - 305 9e-10 Leucine-rich repeat region
LRR_14 PF23598 491 - 556 1e-06 Leucine-rich repeat region
LRR_8 PF13855 502 - 560 1.4e-06 Leucine rich repeat
Pkinase PF00069 688 - 907 2e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 688 - 963 2.5e-35 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012987)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28440
fragaria_vesca FvH4_3g08530 FvH4_3g08530
malus_domestica MD05G1309900.v1.1 MD10G1288500.v1.1
prunus_persica Prupe.4G053100_v2.0.a1
pyrus_communis pycom05g28630
rosa_chinensis RchiOBHm_Chr5g0009311
rosa_laevigata RLG00000031647
rosa_multiflora Rmu_sc0006233.1_g000007
rosa_roxburghii Rroxscaffold_1G00066780 Rroxscaffold_1G00067480
rosa_rugosa Rorug04G0441700
rosa_samantha Rh5AG072200 Rh5BG068700 Rh5CG081100 Rh5DG068900
rosa_wichuraiana Rw5G006750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2005
AbsI CCTCGAGG 1 cut(s) 644
Acc65I GGTACC 1 cut(s) 1872
AccB1I GGYRCC 4 cut(s) 389, 533, 1109, 1872
AccBSI CCGCTC 4 cut(s) 43, 213, 894, 928
AccI GTMKAC 3 cut(s) 516, 2697, 2763
AccII CGCG 4 cut(s) 875, 1285, 1287, 2506
AccIII TCCGGA 1 cut(s) 458
AclWI GGATC 9 cut(s) 104, 607, 620, 1180, 1193, 1552, 1565, 2757, 2985
AcoI YGGCCR 2 cut(s) 481, 751
AcsI RAATTY 1 cut(s) 1782
AcyI GRCGYC 4 cut(s) 141, 186, 390, 2084
AdeI CACNNNGTG 1 cut(s) 666
AfaI GTAC 5 cut(s) 1004, 1292, 1874, 1952, 2099
AfiI CCNNNNNNNGG 4 cut(s) 162, 551, 1743, 2708
AflIII ACRYGT 2 cut(s) 873, 1086
AgeI ACCGGT 1 cut(s) 1242
AgsI TTSAA 6 cut(s) 94, 530, 1465, 1948, 1963, 2789
AhdI GACNNNNNGTC 1 cut(s) 2693
AjiI CACGTC 1 cut(s) 1087
AjnI CCWGG 3 cut(s) 131, 1810, 2922
Alw21I GWGCWC 9 cut(s) 360, 627, 654, 804, 915, 1011, 1155, 1305, 1587
Alw26I GTCTC 7 cut(s) 710, 856, 1486, 2222, 2336, 2680, 2869
AlwI GGATC 9 cut(s) 104, 607, 620, 1180, 1193, 1552, 1565, 2757, 2985
Ama87I CYCGRG 9 cut(s) 353, 497, 604, 644, 1055, 1221, 1496, 1505, 1537
Aor13HI TCCGGA 1 cut(s) 458
AoxI GGCC 9 cut(s) 73, 244, 481, 751, 896, 1469, 2375, 2555, 2830
ApeKI GCWGC 5 cut(s) 829, 1279, 1501, 2405, 2927
ApoI RAATTY 1 cut(s) 1782
ArsI GACNNNNNNTTYG 8 cut(s) 2195, 2227, 2314, 2346, 2480, 2512, 2690, 2722
AseI ATTAAT 2 cut(s) 1370, 2859
AsiGI ACCGGT 1 cut(s) 1242
Asp700I GAANNNNTTC 1 cut(s) 956
Asp718I GGTACC 1 cut(s) 1872
AspLEI GCGC 4 cut(s) 233, 392, 1287, 2506
AvaI CYCGRG 9 cut(s) 353, 497, 604, 644, 1055, 1221, 1496, 1505, 1537
AvaII GGWCC 6 cut(s) 461, 502, 727, 1516, 2197, 2818
BamHI GGATCC 3 cut(s) 612, 1185, 1557
BanI GGYRCC 4 cut(s) 389, 533, 1109, 1872
BanII GRGCYC 7 cut(s) 627, 804, 915, 1011, 1155, 1305, 1587
BarI GAAGNNNNNNTAC 4 cut(s) 522, 554, 1943, 1975
BbsI GAAGAC 1 cut(s) 1343
Bbv12I GWGCWC 9 cut(s) 360, 627, 654, 804, 915, 1011, 1155, 1305, 1587
BbvCI CCTCAGC 1 cut(s) 1854
BbvI GCAGC 5 cut(s) 816, 1266, 1513, 2417, 2914
BccI CCATC 6 cut(s) 317, 743, 1291, 1619, 1826, 1838
BceAI ACGGC 5 cut(s) 748, 757, 1456, 2224, 2506
BciT130I CCWGG 3 cut(s) 133, 1812, 2924
BcoDI GTCTC 7 cut(s) 710, 856, 1486, 2222, 2336, 2680, 2869
BfaI CTAG 3 cut(s) 1092, 1592, 2286
BfoI RGCGCY 2 cut(s) 234, 393
BglII AGATCT 1 cut(s) 2040
BlpI GCTNAGC 3 cut(s) 770, 890, 2111
Bme18I GGWCC 6 cut(s) 461, 502, 727, 1516, 2197, 2818
BmeRI GACNNNNNGTC 1 cut(s) 2693
BmeT110I CYCGRG 9 cut(s) 353, 497, 604, 644, 1055, 1221, 1496, 1505, 1537
BmgBI CACGTC 1 cut(s) 1087
BmiI GGNNCC 9 cut(s) 391, 535, 614, 1111, 1187, 1351, 1559, 1874, 2198
BmrI ACTGGG 2 cut(s) 2030, 2687
BmsI GCATC 4 cut(s) 1902, 2361, 2392, 2959
BmuI ACTGGG 2 cut(s) 2030, 2687
BoxI GACNNNNGTC 1 cut(s) 785
BpiI GAAGAC 1 cut(s) 1343
BplI GAGNNNNNCTC 2 cut(s) 491, 523
BpmI CTGGAG 2 cut(s) 2586, 2690
Bpu10I CCTNAGC 4 cut(s) 1010, 1776, 1854, 1886
Bpu1102I GCTNAGC 3 cut(s) 770, 890, 2111
BpuEI CTTGAG 2 cut(s) 701, 1058
Bsa29I ATCGAT 1 cut(s) 1675
BsaHI GRCGYC 4 cut(s) 141, 186, 390, 2084
BsaJI CCNNGG 5 cut(s) 604, 1221, 2529, 2922, 2973
BsaWI WCCGGW 6 cut(s) 443, 458, 1178, 1242, 1405, 1445
Bsc4I CCNNNNNNNGG 4 cut(s) 162, 551, 1743, 2708
Bse118I RCCGGY 7 cut(s) 242, 386, 749, 821, 918, 973, 1242
Bse1I ACTGG 6 cut(s) 1099, 1111, 1561, 2025, 2551, 2693
Bse3DI GCAATG 3 cut(s) 981, 1341, 2595
BseAI TCCGGA 1 cut(s) 458
BseBI CCWGG 3 cut(s) 133, 1812, 2924
BseCI ATCGAT 1 cut(s) 1675
BseDI CCNNGG 5 cut(s) 604, 1221, 2529, 2922, 2973
BseGI GGATG 3 cut(s) 1837, 2407, 2917
BseLI CCNNNNNNNGG 4 cut(s) 162, 551, 1743, 2708
BseMI GCAATG 3 cut(s) 981, 1341, 2595
BseMII CTCAG 8 cut(s) 784, 851, 881, 1024, 1764, 1845, 2102, 2635
BseNI ACTGG 6 cut(s) 1099, 1111, 1561, 2025, 2551, 2693
BseX3I CGGCCG 2 cut(s) 481, 751
BseXI GCAGC 5 cut(s) 816, 1266, 1513, 2417, 2914
BseYI CCCAGC 2 cut(s) 227, 697
BsgI GTGCAG 4 cut(s) 2180, 2411, 2722, 2824
Bsh1236I CGCG 4 cut(s) 875, 1285, 1287, 2506
Bsh1285I CGRYCG 2 cut(s) 484, 754
BshFI GGCC 9 cut(s) 75, 246, 483, 753, 898, 1471, 2377, 2557, 2832
BshNI GGYRCC 4 cut(s) 389, 533, 1109, 1872
BshTI ACCGGT 1 cut(s) 1242
BshVI ATCGAT 1 cut(s) 1675
BsiEI CGRYCG 2 cut(s) 484, 754
BsiHKAI GWGCWC 9 cut(s) 360, 627, 654, 804, 915, 1011, 1155, 1305, 1587
BsiHKCI CYCGRG 9 cut(s) 353, 497, 604, 644, 1055, 1221, 1496, 1505, 1537
BslFI GGGAC 2 cut(s) 1522, 2303
BslI CCNNNNNNNGG 4 cut(s) 162, 551, 1743, 2708
BsmAI GTCTC 7 cut(s) 710, 856, 1486, 2222, 2336, 2680, 2869
BsmBI CGTCTC 2 cut(s) 710, 2869
BsmFI GGGAC 2 cut(s) 1522, 2303
BsnI GGCC 9 cut(s) 75, 246, 483, 753, 898, 1471, 2377, 2557, 2832
BsoBI CYCGRG 9 cut(s) 353, 497, 604, 644, 1055, 1221, 1496, 1505, 1537
Bsp1286I GDGCHC 9 cut(s) 360, 627, 654, 804, 915, 1011, 1155, 1305, 1587
Bsp13I TCCGGA 1 cut(s) 458
Bsp1407I TGTACA 1 cut(s) 2097
Bsp143I GATC 8 cut(s) 109, 612, 1185, 1557, 1660, 2040, 2749, 2977
Bsp1720I GCTNAGC 3 cut(s) 770, 890, 2111
BspANI GGCC 9 cut(s) 75, 246, 483, 753, 898, 1471, 2377, 2557, 2832
BspCNI CTCAG 8 cut(s) 783, 852, 882, 1023, 1763, 1846, 2103, 2634
BspDI ATCGAT 1 cut(s) 1675
BspEI TCCGGA 1 cut(s) 458
BspFNI CGCG 4 cut(s) 875, 1285, 1287, 2506
BspLI GGNNCC 9 cut(s) 391, 535, 614, 1111, 1187, 1351, 1559, 1874, 2198
BspPI GGATC 9 cut(s) 104, 607, 620, 1180, 1193, 1552, 1565, 2757, 2985
BspQI GCTCTTC 3 cut(s) 1022, 1143, 2630
BspT107I GGYRCC 4 cut(s) 389, 533, 1109, 1872
BsrBI CCGCTC 4 cut(s) 43, 213, 894, 928
BsrDI GCAATG 3 cut(s) 981, 1341, 2595
BsrFI RCCGGY 7 cut(s) 242, 386, 749, 821, 918, 973, 1242
BsrGI TGTACA 1 cut(s) 2097
BsrI ACTGG 6 cut(s) 1099, 1111, 1561, 2025, 2551, 2693
BssAI RCCGGY 7 cut(s) 242, 386, 749, 821, 918, 973, 1242
BssECI CCNNGG 5 cut(s) 604, 1221, 2529, 2922, 2973
BssMI GATC 8 cut(s) 109, 612, 1185, 1557, 1660, 2040, 2749, 2977
BssNI GRCGYC 4 cut(s) 141, 186, 390, 2084
BssT1I CCWWGG 1 cut(s) 2529
Bst2UI CCWGG 3 cut(s) 133, 1812, 2924
Bst4CI ACNGT 4 cut(s) 259, 1526, 2651, 2768
Bst6I CTCTTC 4 cut(s) 14, 1022, 1143, 2630
BstACI GRCGYC 4 cut(s) 141, 186, 390, 2084
BstAUI TGTACA 1 cut(s) 2097
BstEII GGTNACC 1 cut(s) 2677
BstF5I GGATG 3 cut(s) 1837, 2407, 2917
BstFNI CGCG 4 cut(s) 875, 1285, 1287, 2506
BstH2I RGCGCY 2 cut(s) 234, 393
BstHHI GCGC 4 cut(s) 233, 392, 1287, 2506
BstKTI GATC 8 cut(s) 112, 615, 1188, 1560, 1663, 2043, 2752, 2980
BstMAI GTCTC 7 cut(s) 710, 856, 1486, 2222, 2336, 2680, 2869
BstMBI GATC 8 cut(s) 109, 612, 1185, 1557, 1660, 2040, 2749, 2977
BstMCI CGRYCG 2 cut(s) 484, 754
BstNI CCWGG 3 cut(s) 133, 1812, 2924
BstPAI GACNNNNGTC 1 cut(s) 785
BstPI GGTNACC 1 cut(s) 2677
BstUI CGCG 4 cut(s) 875, 1285, 1287, 2506
BstV1I GCAGC 5 cut(s) 816, 1266, 1513, 2417, 2914
BstV2I GAAGAC 1 cut(s) 1343
BstX2I RGATCY 4 cut(s) 612, 1185, 1557, 2040
BstXI CCANNNNNNTGG 1 cut(s) 1106
BstYI RGATCY 4 cut(s) 612, 1185, 1557, 2040
BstZI CGGCCG 2 cut(s) 481, 751
Bsu15I ATCGAT 1 cut(s) 1675
BsuRI GGCC 9 cut(s) 75, 246, 483, 753, 898, 1471, 2377, 2557, 2832
BsuTUI ATCGAT 1 cut(s) 1675
BtgZI GCGATG 1 cut(s) 2193
BtrI CACGTC 1 cut(s) 1087
BtsCI GGATG 3 cut(s) 1837, 2407, 2917
BtsI GCAGTG 2 cut(s) 2122, 2139
BtsIMutI CAGTG 4 cut(s) 1104, 2122, 2139, 2773
CfoI GCGC 4 cut(s) 233, 392, 1287, 2506
Cfr10I RCCGGY 7 cut(s) 242, 386, 749, 821, 918, 973, 1242
Cfr9I CCCGGG 2 cut(s) 604, 1221
ClaI ATCGAT 1 cut(s) 1675
CseI GACGC 7 cut(s) 149, 194, 775, 864, 881, 2008, 2073
Csp6I GTAC 5 cut(s) 1003, 1291, 1873, 1951, 2098
CspAI ACCGGT 1 cut(s) 1242
CviAII CATG 5 cut(s) 880, 2432, 2566, 2872, 2981
CviQI GTAC 5 cut(s) 1003, 1291, 1873, 1951, 2098
DinI GGCGCC 1 cut(s) 391
DpnI GATC 8 cut(s) 111, 614, 1187, 1559, 1662, 2042, 2751, 2979
DpnII GATC 8 cut(s) 109, 612, 1185, 1557, 1660, 2040, 2749, 2977
DraIII CACNNNGTG 1 cut(s) 666
DrdI GACNNNNNNGTC 1 cut(s) 2005
DriI GACNNNNNGTC 1 cut(s) 2693
DseDI GACNNNNNNGTC 1 cut(s) 2005
EaeI YGGCCR 2 cut(s) 481, 751
EagI CGGCCG 2 cut(s) 481, 751
Eam1104I CTCTTC 4 cut(s) 14, 1022, 1143, 2630
Eam1105I GACNNNNNGTC 1 cut(s) 2693
EarI CTCTTC 4 cut(s) 14, 1022, 1143, 2630
EciI GGCGGA 5 cut(s) 109, 635, 750, 2234, 2528
Ecl136II GAGCTC 7 cut(s) 625, 802, 913, 1009, 1153, 1303, 1585
EclXI CGGCCG 2 cut(s) 481, 751
Eco130I CCWWGG 1 cut(s) 2529
Eco147I AGGCCT 1 cut(s) 75
Eco24I GRGCYC 7 cut(s) 627, 804, 915, 1011, 1155, 1305, 1587
Eco32I GATATC 1 cut(s) 1332
Eco47I GGWCC 6 cut(s) 461, 502, 727, 1516, 2197, 2818
Eco52I CGGCCG 2 cut(s) 481, 751
Eco53kI GAGCTC 7 cut(s) 625, 802, 913, 1009, 1153, 1303, 1585
Eco88I CYCGRG 9 cut(s) 353, 497, 604, 644, 1055, 1221, 1496, 1505, 1537
Eco91I GGTNACC 1 cut(s) 2677
EcoICRI GAGCTC 7 cut(s) 625, 802, 913, 1009, 1153, 1303, 1585
EcoO109I RGGNCCY 3 cut(s) 502, 2555, 2818
EcoO65I GGTNACC 1 cut(s) 2677
EcoRII CCWGG 3 cut(s) 131, 1810, 2922
EcoRV GATATC 1 cut(s) 1332
EcoT14I CCWWGG 1 cut(s) 2529
EcoT22I ATGCAT 1 cut(s) 2614
EcoT38I GRGCYC 7 cut(s) 627, 804, 915, 1011, 1155, 1305, 1587
EgeI GGCGCC 1 cut(s) 391
EheI GGCGCC 1 cut(s) 391
ErhI CCWWGG 1 cut(s) 2529
Esp3I CGTCTC 2 cut(s) 710, 2869
FaeI CATG 5 cut(s) 883, 2435, 2569, 2875, 2984
FalI AAGNNNNNCTT 1 cut(s) 2977
FaqI GGGAC 2 cut(s) 1522, 2303
FatI CATG 5 cut(s) 879, 2431, 2565, 2871, 2980
FauI CCCGC 4 cut(s) 406, 887, 1258, 2072
FblI GTMKAC 3 cut(s) 516, 2697, 2763
FokI GGATG 3 cut(s) 1844, 2414, 2904
FriOI GRGCYC 7 cut(s) 627, 804, 915, 1011, 1155, 1305, 1587
FspBI CTAG 3 cut(s) 1092, 1592, 2286
GlaI GCGC 4 cut(s) 232, 391, 1286, 2505
GsaI CCCAGC 2 cut(s) 231, 701
GsuI CTGGAG 2 cut(s) 2586, 2690
HaeII RGCGCY 2 cut(s) 234, 393
HaeIII GGCC 9 cut(s) 75, 246, 483, 753, 898, 1471, 2377, 2557, 2832
HgaI GACGC 7 cut(s) 149, 194, 775, 864, 881, 2008, 2073
HhaI GCGC 4 cut(s) 233, 392, 1287, 2506
Hin1I GRCGYC 4 cut(s) 141, 186, 390, 2084
Hin1II CATG 5 cut(s) 883, 2435, 2569, 2875, 2984
Hin6I GCGC 4 cut(s) 231, 390, 1285, 2504
HinP1I GCGC 4 cut(s) 231, 390, 1285, 2504
HincII GTYRAC 2 cut(s) 2698, 2776
HindII GTYRAC 2 cut(s) 2698, 2776
HindIII AAGCTT 2 cut(s) 2141, 2264
Hpy166II GTNNAC 7 cut(s) 517, 1104, 1291, 2098, 2698, 2764, 2776
Hpy188I TCNGA 9 cut(s) 612, 636, 795, 1022, 1401, 2624, 2711, 2754, 2977
Hpy8I GTNNAC 7 cut(s) 517, 1104, 1291, 2098, 2698, 2764, 2776
HpyAV CCTTC 6 cut(s) 65, 389, 487, 524, 1960, 2086
HpyCH4III ACNGT 4 cut(s) 259, 1526, 2651, 2768
HpyCH4IV ACGT 1 cut(s) 1086
HpySE526I ACGT 1 cut(s) 1086
Hsp92I GRCGYC 4 cut(s) 141, 186, 390, 2084
Hsp92II CATG 5 cut(s) 883, 2435, 2569, 2875, 2984
HspAI GCGC 4 cut(s) 231, 390, 1285, 2504
KasI GGCGCC 1 cut(s) 389
Kpn2I TCCGGA 1 cut(s) 458
KpnI GGTACC 1 cut(s) 1876
KroI GCCGGC 2 cut(s) 242, 973
KroNI GCCGGC 2 cut(s) 244, 975
Kzo9I GATC 8 cut(s) 109, 612, 1185, 1557, 1660, 2040, 2749, 2977
LguI GCTCTTC 3 cut(s) 1022, 1143, 2630
LmnI GCTCC 9 cut(s) 48, 218, 622, 966, 1300, 1349, 2501, 2605, 2671
Lsp1109I GCAGC 5 cut(s) 816, 1266, 1513, 2417, 2914
LweI GCATC 4 cut(s) 1902, 2361, 2392, 2959
MaeI CTAG 3 cut(s) 1092, 1592, 2286
MaeII ACGT 1 cut(s) 1086
MaeIII GTNAC 8 cut(s) 1235, 1526, 1598, 2542, 2591, 2677, 2795, 2949
MalI GATC 8 cut(s) 111, 614, 1187, 1559, 1662, 2042, 2751, 2979
MbiI CCGCTC 4 cut(s) 43, 213, 894, 928
MboI GATC 8 cut(s) 109, 612, 1185, 1557, 1660, 2040, 2749, 2977
MfeI CAATTG 2 cut(s) 160, 1551
MflI RGATCY 4 cut(s) 612, 1185, 1557, 2040
MhlI GDGCHC 9 cut(s) 360, 627, 654, 804, 915, 1011, 1155, 1305, 1587
MluI ACGCGT 1 cut(s) 873
Mly113I GGCGCC 1 cut(s) 390
MmeI TCCRAC 5 cut(s) 109, 590, 1761, 2466, 2496
Mph1103I ATGCAT 1 cut(s) 2614
MroI TCCGGA 1 cut(s) 458
MroNI GCCGGC 2 cut(s) 242, 973
MroXI GAANNNNTTC 1 cut(s) 956
MseI TTAA 9 cut(s) 746, 818, 1247, 1370, 1650, 1728, 1860, 2388, 2859
MslI CAYNNNNRTG 1 cut(s) 2460
MspA1I CMGCKG 1 cut(s) 889
MunI CAATTG 2 cut(s) 160, 1551
MvaI CCWGG 3 cut(s) 133, 1812, 2924
MvnI CGCG 4 cut(s) 875, 1285, 1287, 2506
NaeI GCCGGC 2 cut(s) 244, 975
NarI GGCGCC 1 cut(s) 390
NdeII GATC 8 cut(s) 109, 612, 1185, 1557, 1660, 2040, 2749, 2977
NgoMIV GCCGGC 2 cut(s) 242, 973
NlaIII CATG 5 cut(s) 883, 2435, 2569, 2875, 2984
NlaIV GGNNCC 9 cut(s) 391, 535, 614, 1111, 1187, 1351, 1559, 1874, 2198
NmeAIII GCCGAG 2 cut(s) 531, 606
NmuCI GTSAC 4 cut(s) 1235, 2542, 2677, 2949
NsiI ATGCAT 1 cut(s) 2614
PaeR7I CTCGAG 3 cut(s) 353, 497, 644
PceI AGGCCT 1 cut(s) 75
PciSI GCTCTTC 3 cut(s) 1022, 1143, 2630
PcsI WCGNNNNNNNCGW 2 cut(s) 966, 2006
PdiI GCCGGC 2 cut(s) 244, 975
PdmI GAANNNNTTC 1 cut(s) 956
PfeI GAWTC 5 cut(s) 685, 757, 1429, 1672, 2789
PfoI TCCNGGA 5 cut(s) 131, 1610, 1667, 1682, 2087
PinAI ACCGGT 1 cut(s) 1242
PluTI GGCGCC 1 cut(s) 393
PpuMI RGGWCCY 2 cut(s) 502, 2818
PshAI GACNNNNGTC 1 cut(s) 785
PshBI ATTAAT 2 cut(s) 1370, 2859
Psp124BI GAGCTC 7 cut(s) 627, 804, 915, 1011, 1155, 1305, 1587
Psp5II RGGWCCY 2 cut(s) 502, 2818
Psp6I CCWGG 3 cut(s) 131, 1810, 2922
PspEI GGTNACC 1 cut(s) 2677
PspFI CCCAGC 2 cut(s) 227, 697
PspGI CCWGG 3 cut(s) 131, 1810, 2922
PspN4I GGNNCC 9 cut(s) 391, 535, 614, 1111, 1187, 1351, 1559, 1874, 2198
PspPPI RGGWCCY 2 cut(s) 502, 2818
PspXI VCTCGAGB 2 cut(s) 497, 644
PsuI RGATCY 4 cut(s) 612, 1185, 1557, 2040
PvuII CAGCTG 1 cut(s) 889
RsaI GTAC 5 cut(s) 1004, 1292, 1874, 1952, 2099
RsaNI GTAC 5 cut(s) 1003, 1291, 1873, 1951, 2098
RseI CAYNNNNRTG 1 cut(s) 2460
SacI GAGCTC 7 cut(s) 627, 804, 915, 1011, 1155, 1305, 1587
SalI GTCGAC 1 cut(s) 2696
SapI GCTCTTC 3 cut(s) 1022, 1143, 2630
SaqAI TTAA 9 cut(s) 746, 818, 1247, 1370, 1650, 1728, 1860, 2388, 2859
Sau3AI GATC 8 cut(s) 109, 612, 1185, 1557, 1660, 2040, 2749, 2977
SduI GDGCHC 9 cut(s) 360, 627, 654, 804, 915, 1011, 1155, 1305, 1587
SfaNI GCATC 4 cut(s) 1902, 2361, 2392, 2959
SfoI GGCGCC 1 cut(s) 391
Sfr274I CTCGAG 3 cut(s) 353, 497, 644
SgrAI CRCCGGYG 1 cut(s) 386
SinI GGWCC 6 cut(s) 461, 502, 727, 1516, 2197, 2818
SlaI CTCGAG 3 cut(s) 353, 497, 644
SmaI CCCGGG 2 cut(s) 606, 1223
SmiMI CAYNNNNRTG 1 cut(s) 2460
SmlI CTYRAG 5 cut(s) 353, 497, 644, 716, 1073
SmoI CTYRAG 5 cut(s) 353, 497, 644, 716, 1073
SseBI AGGCCT 1 cut(s) 75
SspDI GGCGCC 1 cut(s) 389
SspMI CTAG 3 cut(s) 1092, 1592, 2286
SstI GAGCTC 7 cut(s) 627, 804, 915, 1011, 1155, 1305, 1587
StuI AGGCCT 1 cut(s) 75
StyI CCWWGG 1 cut(s) 2529
TaaI ACNGT 4 cut(s) 259, 1526, 2651, 2768
TaiI ACGT 1 cut(s) 1089
TatI WGTACW 3 cut(s) 1290, 1950, 2097
TauI GCSGC 5 cut(s) 270, 486, 835, 928, 1285
TfiI GAWTC 5 cut(s) 685, 757, 1429, 1672, 2789
Tru1I TTAA 9 cut(s) 746, 818, 1247, 1370, 1650, 1728, 1860, 2388, 2859
Tru9I TTAA 9 cut(s) 746, 818, 1247, 1370, 1650, 1728, 1860, 2388, 2859
TscAI CASTG 4 cut(s) 1111, 2122, 2139, 2773
TseFI GTSAC 4 cut(s) 1235, 2542, 2677, 2949
TseI GCWGC 5 cut(s) 829, 1279, 1501, 2405, 2927
Tsp45I GTSAC 4 cut(s) 1235, 2542, 2677, 2949
TspDTI ATGAA 5 cut(s) 896, 1727, 1888, 2073, 2444
TspGWI ACGGA 2 cut(s) 1633, 2856
TspMI CCCGGG 2 cut(s) 604, 1221
TspRI CASTG 4 cut(s) 1111, 2122, 2139, 2773
VpaK11BI GGWCC 6 cut(s) 461, 502, 727, 1516, 2197, 2818
VspI ATTAAT 2 cut(s) 1370, 2859
XapI RAATTY 1 cut(s) 1782
XhoI CTCGAG 3 cut(s) 353, 497, 644
XmaI CCCGGG 2 cut(s) 604, 1221
XmiI GTMKAC 3 cut(s) 516, 2697, 2763
XmnI GAANNNNTTC 1 cut(s) 956
XspI CTAG 3 cut(s) 1092, 1592, 2286
Zsp2I ATGCAT 1 cut(s) 2614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.