Rh5CG081100
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0012987
Physical Location & Seq
Forward (+)
6235192 .. 6240120
4929 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG081100.1

Sequence Viewer

Length: 2991 bp
ATGAAGACAAAAGTGCTTCTGTTCTTCCTCCTCCCTCTCCTCCTCTTCTCCTCCACCTCCTCCTCCCTCACCCAAGAAGGCCTCTACCTCCAACACATCAAACACTCCCTCTCCGACCCCGACTCCTCCCTCTCCTCCTGGAACGACCAAGACGCCACCCCTTGCTCATGGGACGGCATCACATGCAACCCTTCCTCCCACGTCATCACCTCCGTCGACCTCTCCGGCTTCAACCTCTTCGGCCCTTTCCCCTCCCTCCTCTGCCGCCTCCCCAACCTCACCTTCCTCTCCCTCTACAACAACTCCATCAACTCCACCCTACCCTCCGACATCTCCACGTGTCAGAACCTCCAACACCTCGACCTCTCCCAGAACCTCCTCACCGGCTCCCTCCCCTCCAACCTTGCCGACCTCCCCAACCTCCGCTACCTCGACCTCACCGGCAACAACTTCTCGGGAGAAATTCCCGAGTCTTTCGGGAGATTCCAAAAACTCGAGGTCCTTTCTCTCGTCTACAACCTCCTCGACAGCGCCCTGCCCCCATTTCTCGGCAATATCTCGTCCCTCAAAATGCTCAACCTTTCTTACAACCCGTTTTTCCCGGGTCGGATCCCGCCCGAGCTCGGAAACTTAACGAACCTCGAGGTCCTCTGGCTGACCGAGTGTAACTTAGTCGGCGAAATACCCGACTCGCTGGGTCGACTCAGTAAACTCACCGATTTGGACCTCGCCATTAACGCCCTGCACGGCTCGATTCCGGCGTCGTTGACCGAGTTGACCAGCGTGGTTCAGATTGAGCTCTATAACAACACCTTGACCGGAGGCCTCCCGCCGGGAATGTCGAAGCTGAAGAATCTCCGGCTGCTCGACGCGTCCATGAACCGGCTGACGGGGCCGATTCCCGACGAGTTGACTCAGCTGGAGCTGGAAAGCCTGAATCTTTACGAGAACAACTTCGAGGGGAGTTTGCCGGCGAGCATTGCCGACTCGCCGAACCTGTACGAGCTCCGGCTGTTTCAGAACAAGCTCACCGGCGAGCTGCCGCGGAATCTCGGCCAGAACTCGCCGCTGAGGTGGATAGACCTGTCGAACAACCAGTTTTCCGGGAGTATTCCGGCGAGTCTATGCGAAAAGGGGCAAATTGAGGAGATGCTGATGATCAACAATGCTTTTTCCGGCGAGATTCCGGCGAGTCTGGGGGAGTGTCAGAGCTTGACACGTGTCCGGTTGGGTCACAACCGGTTGAGCGGTGAAGTTCCGGCTAGTTTCTGGGGGCTGCCGCATGTCTATTTGCTCGAGCTGGTTGATAACCAGTTATCCGGGCCGATAGCCAAGACTATTGCCGGTGCGGCTAACCTTTCGCTTCTGATAATCGAAAAGAACATGTTTGAAGGTTCGATACCGGAGGAAATTGGGTGGGTTGAAAATCTTCTGCAGTTTTCCGGCGGTGAGAATAGGTTCAGTGGGCCTTTGCCGGAGACAATAGTTAGGTTACGACATCTTAGCACGCTGGACCTGCACAACAATGAGCTTTCCGGTGAGTTTCCGAGTGGGAGTCAGTCTTTGGTTCGGCTCAATGAACTCAACTTGAAGCATAACCAACTGTCCGGGAAAATCCCAGATGGAATTGGGAACATGTCTTCGCTGAATTACCTGGATCTCTCCGGGAATCGGTTTTCGGGCCAAGTTCCGGTTTGGTTGCAGAATATGAAGATCAATGTGTTCAATTTGTCGGACAACCAGCTTTCGGGTGAGCTCCCTCCTTTGTTTGCGAAGGAGATGTACAGAAATAGCTTTCTGGGAAATCCGGGTCTTTGTGGAGATTTGGAGGGCTTGTGTGAGAGCAGAGCCGAGGAGAAAACCCAGGGCTACATATGGTTGCTGAGGTGCATTTTCATTCTGGCTGCCTTGGTGTTTGTGGTGGGTGTGGTTTGGTTTTACTTCAAGTACAAGAACTTCAAGAAGGCCAGCGGTGCTATAGATAAATCCAAGTGGACTTTGATGTCGTTTCACAAGCTGGGTTTCAGCGAGTATGAGATACTGGACTGCCTTGATGAAGATAATGTGATTGGGACTGGGGGGTCTGGGAAAGTGTACAAGGTTGTGCTGAGCAGTGGTGAGGCTGTTGCAGTGAAAAAGCTGTGGAGAGGGAAAGTGAAAGACTGTGAAAGCGATGATGTTGAGAAAGGTTGGGTCCAAGATGATGGCTTCGAGGCAGAGGTCGACACTTTGGGGAGGATTAGGCACAAGAATATTGTGAAGCTTTGGTGCTGTTGTACCGCAAGAGATTGCAAGCTTTTGGTGTATGAGTACATGCCAAATGGTAGTCTTGGTGATTTGTTGCATAGTAGCAAAGCAGGGTTGCTGGATTGGCCTACAAGGTTCAAGATTGCTCTTGATGCTGCTGATGGGCTTTCGTATCTGCACCATGATTGTGTTCCTGCTATTGTTCATAGAGATGTGAAGTCAAACAATATATTGTTGGATGGGGATTTCGGAGCCAGAGTAGCGGATTTTGGAGTAGCTAAGGTCGTTGATGCAACTGGAAAAGGGGCTAAATCCATGTCGGTCATTGCAGGTTCTTGTGGTTACATTGCTCCAGAATATGCATACACACTGAGGGTGAATGAGAAGAGTGACATCTACAGTTTTGGTGTGGTGATTCTGGAATTGGTTACCGGGAAACTTCCAGTTGACCCAGAATTCGGAGAAAAGGACTTGGTGAAGTGGGTCTGTACCACCTTAGATCAGAAAGGAGTGGACCATGTCATTGACCCCAAACTCGATTCTTGTTACAAGGAAGAAGTATGCAAGGTCCTCAATATCGGGCTCCTCTGCACCAGTCCTCTCCCAATCAATAGACCATCGATGAGAAGAGTGGTGAAGTTGTTGCAAGAAGCGGGAACGGAGAAGCATCCTCAGATTAAGAAGGAAGGCAAATTGTCACCATATTACTATGAAGATGCATCGGATCATGGAAGTGTAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

996

Amino Acids

109.8

Weight (kDa)

5.2

Isoelectric Point (pI)

36.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 24 - 63 1.1e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 87 - 191 1.5e-08 Leucine-rich repeat region
LRR_8 PF13855 91 - 151 1.4e-08 Leucine rich repeat
LRR_4 PF12799 115 - 155 2e-08 Leucine Rich repeats (2 copies)
LRR_14 PF23598 134 - 292 1.1e-12 Leucine-rich repeat region
LRR_14 PF23598 182 - 338 1.3e-08 Leucine-rich repeat region
LRR_14 PF23598 490 - 555 2.9e-08 Leucine-rich repeat region
LRR_8 PF13855 499 - 559 1.1e-08 Leucine rich repeat
Pkinase PF00069 687 - 912 3.9e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 687 - 962 2.2e-35 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012987)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28440
fragaria_vesca FvH4_3g08530 FvH4_3g08530
malus_domestica MD05G1309900.v1.1 MD10G1288500.v1.1
prunus_persica Prupe.4G053100_v2.0.a1
pyrus_communis pycom05g28630
rosa_chinensis RchiOBHm_Chr5g0009311
rosa_laevigata RLG00000031647
rosa_multiflora Rmu_sc0006233.1_g000007
rosa_roxburghii Rroxscaffold_1G00066780 Rroxscaffold_1G00067480
rosa_rugosa Rorug04G0441700
rosa_samantha Rh5AG072200 Rh5BG068700 Rh5CG081100 Rh5DG068900
rosa_wichuraiana Rw5G006750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 2002, 2080
AbsI CCTCGAGG 1 cut(s) 641
Acc36I ACCTGC 2 cut(s) 1524, 2567
AccBSI CCGCTC 1 cut(s) 1248
AccI GTMKAC 4 cut(s) 216, 513, 700, 2223
AccII CGCG 2 cut(s) 872, 1045
AclWI GGATC 4 cut(s) 604, 617, 1665, 2979
AcoI YGGCCR 1 cut(s) 1054
AcsI RAATTY 2 cut(s) 462, 2702
AcuI CTGAAG 1 cut(s) 869
AcvI CACGTG 2 cut(s) 339, 1220
AcyI GRCGYC 2 cut(s) 153, 761
AfaI GTAC 7 cut(s) 1001, 1784, 1949, 2096, 2278, 2312, 2737
AfiI CCNNNNNNNGG 9 cut(s) 548, 623, 832, 882, 889, 1671, 1690, 1747, 2705
AflIII ACRYGT 6 cut(s) 338, 870, 1217, 1219, 1383, 1635
AgeI ACCGGT 1 cut(s) 1239
AgsI TTSAA 8 cut(s) 232, 1391, 1424, 1591, 1726, 1945, 1960, 2386
AjiI CACGTC 1 cut(s) 202
AjnI CCWGG 3 cut(s) 137, 1653, 1863
AjuI GAANNNNNNNTTGG 2 cut(s) 266, 298
Alw21I GWGCWC 4 cut(s) 624, 801, 1008, 1758
Alw26I GTCTC 1 cut(s) 1472
AlwI GGATC 4 cut(s) 604, 617, 1665, 2979
Ama87I CYCGRG 7 cut(s) 454, 467, 494, 601, 617, 641, 1295
ApeKI GCWGC 5 cut(s) 862, 1039, 1276, 1904, 2402
ApoI RAATTY 2 cut(s) 462, 2702
ArsI GACNNNNNNTTYG 2 cut(s) 2687, 2719
AsiGI ACCGGT 1 cut(s) 1239
Asp700I GAANNNNTTC 3 cut(s) 953, 1428, 1457
AspLEI GCGC 1 cut(s) 533
AsuC2I CCSGG 9 cut(s) 602, 603, 834, 1105, 1321, 1609, 1666, 1809, 2680
AvaI CYCGRG 7 cut(s) 454, 467, 494, 601, 617, 641, 1295
AvaII GGWCC 7 cut(s) 499, 646, 724, 1513, 2194, 2761, 2815
BamHI GGATCC 1 cut(s) 609
BanII GRGCYC 5 cut(s) 624, 801, 1008, 1758, 2832
BarI GAAGNNNNNNTAC 6 cut(s) 1383, 1415, 1766, 1798, 1940, 1972
BbrPI CACGTG 2 cut(s) 339, 1220
BbsI GAAGAC 2 cut(s) 11, 1632
Bbv12I GWGCWC 4 cut(s) 624, 801, 1008, 1758
BbvCI CCTCAGC 2 cut(s) 1070, 1883
BbvI GCAGC 5 cut(s) 849, 1026, 1263, 1891, 2389
BccI CCATC 6 cut(s) 314, 1616, 2198, 2402, 2480, 2872
BceAI ACGGC 2 cut(s) 190, 763
BciT130I CCWGG 3 cut(s) 139, 1655, 1865
BclI TGATCA 1 cut(s) 1158
BcnI CCSGG 9 cut(s) 602, 603, 834, 1105, 1321, 1609, 1666, 1809, 2680
BcoDI GTCTC 1 cut(s) 1472
BfaI CTAG 1 cut(s) 1263
BfmI CTRYAG 3 cut(s) 1433, 1977, 2644
BfoI RGCGCY 1 cut(s) 534
BfuAI ACCTGC 2 cut(s) 1524, 2567
BglI GCCNNNNNGGC 1 cut(s) 1349
BlpI GCTNAGC 1 cut(s) 2108
Bme18I GGWCC 7 cut(s) 499, 646, 724, 1513, 2194, 2761, 2815
BmeT110I CYCGRG 7 cut(s) 454, 467, 494, 601, 617, 641, 1295
BmgBI CACGTC 1 cut(s) 202
BmiI GGNNCC 6 cut(s) 388, 611, 894, 2195, 2500, 2831
BmrI ACTGGG 1 cut(s) 2085
BmsI GCATC 7 cut(s) 186, 1140, 2389, 2527, 2923, 2953, 2975
BmuI ACTGGG 1 cut(s) 2085
BoxI GACNNNNGTC 1 cut(s) 1220
BpiI GAAGAC 2 cut(s) 11, 1632
BpmI CTGGAG 2 cut(s) 941, 2583
Bpu10I CCTNAGC 3 cut(s) 1070, 1883, 2526
Bpu1102I GCTNAGC 1 cut(s) 2108
BpuMI CCSGG 9 cut(s) 602, 603, 834, 1105, 1321, 1609, 1666, 1809, 2680
Bsa29I ATCGAT 1 cut(s) 2867
BsaAI YACGTR 2 cut(s) 339, 1220
BsaHI GRCGYC 2 cut(s) 153, 761
BsaJI CCNNGG 6 cut(s) 601, 1043, 1851, 1863, 1864, 1908
BsaWI WCCGGW 6 cut(s) 818, 1224, 1239, 1402, 1535, 1690
Bsc4I CCNNNNNNNGG 9 cut(s) 548, 623, 832, 882, 889, 1671, 1690, 1747, 2705
Bse118I RCCGGY 7 cut(s) 383, 440, 882, 970, 1031, 1239, 1343
Bse1I ACTGG 7 cut(s) 1096, 1312, 2046, 2080, 2548, 2690, 2841
Bse3DI GCAATG 3 cut(s) 978, 2571, 2592
BseBI CCWGG 3 cut(s) 139, 1655, 1865
BseCI ATCGAT 1 cut(s) 2867
BseDI CCNNGG 6 cut(s) 601, 1043, 1851, 1863, 1864, 1908
BseGI GGATG 2 cut(s) 2491, 2914
BseLI CCNNNNNNNGG 9 cut(s) 548, 623, 832, 882, 889, 1671, 1690, 1747, 2705
BseMI GCAATG 3 cut(s) 978, 2571, 2592
BseMII CTCAG 7 cut(s) 718, 929, 1061, 1874, 2099, 2609, 2933
BseNI ACTGG 7 cut(s) 1096, 1312, 2046, 2080, 2548, 2690, 2841
BseXI GCAGC 5 cut(s) 849, 1026, 1263, 1891, 2389
BseYI CCCAGC 2 cut(s) 694, 2017
BsgI GTGCAG 4 cut(s) 728, 1502, 2408, 2821
Bsh1236I CGCG 2 cut(s) 872, 1045
BshTI ACCGGT 1 cut(s) 1239
BshVI ATCGAT 1 cut(s) 2867
BsiHKAI GWGCWC 4 cut(s) 624, 801, 1008, 1758
BsiHKCI CYCGRG 7 cut(s) 454, 467, 494, 601, 617, 641, 1295
BslFI GGGAC 3 cut(s) 185, 547, 2086
BslI CCNNNNNNNGG 9 cut(s) 548, 623, 832, 882, 889, 1671, 1690, 1747, 2705
BsmAI GTCTC 1 cut(s) 1472
BsmFI GGGAC 3 cut(s) 185, 547, 2086
BsoBI CYCGRG 7 cut(s) 454, 467, 494, 601, 617, 641, 1295
Bsp1286I GDGCHC 5 cut(s) 624, 801, 1008, 1758, 2832
Bsp1407I TGTACA 2 cut(s) 1782, 2094
Bsp143I GATC 6 cut(s) 609, 1158, 1657, 1713, 2746, 2971
Bsp1720I GCTNAGC 1 cut(s) 2108
BspCNI CTCAG 7 cut(s) 717, 928, 1062, 1875, 2100, 2610, 2932
BspDI ATCGAT 1 cut(s) 2867
BspFNI CGCG 2 cut(s) 872, 1045
BspLI GGNNCC 6 cut(s) 388, 611, 894, 2195, 2500, 2831
BspMAI CTGCAG 1 cut(s) 1437
BspMI ACCTGC 2 cut(s) 1524, 2567
BspPI GGATC 4 cut(s) 604, 617, 1665, 2979
BsrBI CCGCTC 1 cut(s) 1248
BsrDI GCAATG 3 cut(s) 978, 2571, 2592
BsrFI RCCGGY 7 cut(s) 383, 440, 882, 970, 1031, 1239, 1343
BsrGI TGTACA 2 cut(s) 1782, 2094
BsrI ACTGG 7 cut(s) 1096, 1312, 2046, 2080, 2548, 2690, 2841
BssAI RCCGGY 7 cut(s) 383, 440, 882, 970, 1031, 1239, 1343
BssECI CCNNGG 6 cut(s) 601, 1043, 1851, 1863, 1864, 1908
BssMI GATC 6 cut(s) 609, 1158, 1657, 1713, 2746, 2971
BssNI GRCGYC 2 cut(s) 153, 761
BssT1I CCWWGG 1 cut(s) 1908
Bst2UI CCWGG 3 cut(s) 139, 1655, 1865
Bst4CI ACNGT 3 cut(s) 1605, 2165, 2648
Bst6I CTCTTC 4 cut(s) 50, 242, 2627, 2869
BstACI GRCGYC 2 cut(s) 153, 761
BstAPI GCANNNNNTGC 1 cut(s) 183
BstAUI TGTACA 2 cut(s) 1782, 2094
BstBAI YACGTR 2 cut(s) 339, 1220
BstC8I GCNNGC 6 cut(s) 972, 976, 1037, 1508, 1969, 2294
BstDSI CCRYGG 1 cut(s) 1043
BstEII GGTNACC 1 cut(s) 2674
BstF5I GGATG 2 cut(s) 2491, 2914
BstFNI CGCG 2 cut(s) 872, 1045
BstH2I RGCGCY 1 cut(s) 534
BstHHI GCGC 1 cut(s) 533
BstKTI GATC 6 cut(s) 612, 1161, 1660, 1716, 2749, 2974
BstMAI GTCTC 1 cut(s) 1472
BstMBI GATC 6 cut(s) 609, 1158, 1657, 1713, 2746, 2971
BstNI CCWGG 3 cut(s) 139, 1655, 1865
BstNSI RCATGY 5 cut(s) 186, 1286, 1387, 1639, 2317
BstPAI GACNNNNGTC 1 cut(s) 1220
BstPI GGTNACC 1 cut(s) 2674
BstSFI CTRYAG 3 cut(s) 1433, 1977, 2644
BstUI CGCG 2 cut(s) 872, 1045
BstV1I GCAGC 5 cut(s) 849, 1026, 1263, 1891, 2389
BstV2I GAAGAC 2 cut(s) 11, 1632
BstX2I RGATCY 2 cut(s) 609, 1657
BstXI CCANNNNNNTGG 1 cut(s) 2204
BstYI RGATCY 2 cut(s) 609, 1657
Bsu15I ATCGAT 1 cut(s) 2867
BsuTUI ATCGAT 1 cut(s) 2867
BtgI CCRYGG 1 cut(s) 1043
BtgZI GCGATG 1 cut(s) 2187
BtrI CACGTC 1 cut(s) 202
BtsCI GGATG 2 cut(s) 2491, 2914
BtsI GCAGTG 2 cut(s) 2119, 2136
BtsIMutI CAGTG 4 cut(s) 1468, 2119, 2136, 2615
BveI ACCTGC 2 cut(s) 1524, 2567
Cac8I GCNNGC 6 cut(s) 972, 976, 1037, 1508, 1969, 2294
CfoI GCGC 1 cut(s) 533
Cfr10I RCCGGY 7 cut(s) 383, 440, 882, 970, 1031, 1239, 1343
Cfr42I CCGCGG 1 cut(s) 1046
Cfr9I CCCGGG 1 cut(s) 601
ClaI ATCGAT 1 cut(s) 2867
CseI GACGC 4 cut(s) 161, 750, 861, 878
Csp6I GTAC 7 cut(s) 1000, 1783, 1948, 2095, 2277, 2311, 2736
CspAI ACCGGT 1 cut(s) 1239
CviQI GTAC 7 cut(s) 1000, 1783, 1948, 2095, 2277, 2311, 2736
DpnI GATC 6 cut(s) 611, 1160, 1659, 1715, 2748, 2973
DpnII GATC 6 cut(s) 609, 1158, 1657, 1713, 2746, 2971
DrdI GACNNNNNNGTC 2 cut(s) 2002, 2080
DseDI GACNNNNNNGTC 2 cut(s) 2002, 2080
EaeI YGGCCR 1 cut(s) 1054
Eam1104I CTCTTC 4 cut(s) 50, 242, 2627, 2869
EarI CTCTTC 4 cut(s) 50, 242, 2627, 2869
Ecl136II GAGCTC 4 cut(s) 622, 799, 1006, 1756
Eco130I CCWWGG 1 cut(s) 1908
Eco147I AGGCCT 2 cut(s) 81, 825
Eco24I GRGCYC 5 cut(s) 624, 801, 1008, 1758, 2832
Eco47I GGWCC 7 cut(s) 499, 646, 724, 1513, 2194, 2761, 2815
Eco53kI GAGCTC 4 cut(s) 622, 799, 1006, 1756
Eco57I CTGAAG 1 cut(s) 869
Eco72I CACGTG 2 cut(s) 339, 1220
Eco88I CYCGRG 7 cut(s) 454, 467, 494, 601, 617, 641, 1295
Eco91I GGTNACC 1 cut(s) 2674
EcoICRI GAGCTC 4 cut(s) 622, 799, 1006, 1756
EcoO109I RGGNCCY 3 cut(s) 499, 646, 2815
EcoO65I GGTNACC 1 cut(s) 2674
EcoRI GAATTC 1 cut(s) 2702
EcoRII CCWGG 3 cut(s) 137, 1653, 1863
EcoT14I CCWWGG 1 cut(s) 1908
EcoT22I ATGCAT 2 cut(s) 2611, 2968
EcoT38I GRGCYC 5 cut(s) 624, 801, 1008, 1758, 2832
ErhI CCWWGG 1 cut(s) 1908
FalI AAGNNNNNCTT 1 cut(s) 2971
FaqI GGGAC 3 cut(s) 185, 547, 2086
FauI CCCGC 3 cut(s) 621, 837, 2893
FauNDI CATATG 1 cut(s) 1874
FbaI TGATCA 1 cut(s) 1158
FblI GTMKAC 4 cut(s) 216, 513, 700, 2223
FokI GGATG 2 cut(s) 2498, 2901
FriOI GRGCYC 5 cut(s) 624, 801, 1008, 1758, 2832
FspBI CTAG 1 cut(s) 1263
GlaI GCGC 1 cut(s) 532
GsaI CCCAGC 2 cut(s) 698, 2021
GsuI CTGGAG 2 cut(s) 941, 2583
HaeII RGCGCY 1 cut(s) 534
HgaI GACGC 4 cut(s) 161, 750, 861, 878
HhaI GCGC 1 cut(s) 533
Hin1I GRCGYC 2 cut(s) 153, 761
Hin6I GCGC 1 cut(s) 531
HinP1I GCGC 1 cut(s) 531
HincII GTYRAC 7 cut(s) 217, 701, 768, 777, 912, 2224, 2695
HindII GTYRAC 7 cut(s) 217, 701, 768, 777, 912, 2224, 2695
HindIII AAGCTT 2 cut(s) 2261, 2294
Hpy188III TCNNGA 9 cut(s) 456, 467, 478, 902, 1960, 2386, 2396, 2600, 2666
Hpy99I CGWCG 4 cut(s) 218, 766, 872, 908
HpyAV CCTTC 8 cut(s) 71, 201, 292, 1385, 1768, 1957, 2923, 2927
HpyCH4III ACNGT 3 cut(s) 1605, 2165, 2648
HpyCH4IV ACGT 3 cut(s) 201, 338, 1219
HpySE526I ACGT 3 cut(s) 201, 338, 1219
Hsp92I GRCGYC 2 cut(s) 153, 761
HspAI GCGC 1 cut(s) 531
KroI GCCGGC 1 cut(s) 970
KroNI GCCGGC 1 cut(s) 972
Ksp22I TGATCA 1 cut(s) 1158
KspI CCGCGG 1 cut(s) 1046
Kzo9I GATC 6 cut(s) 609, 1158, 1657, 1713, 2746, 2971
LmnI GCTCC 7 cut(s) 392, 922, 1011, 1761, 2498, 2602, 2835
Lsp1109I GCAGC 5 cut(s) 849, 1026, 1263, 1891, 2389
LweI GCATC 7 cut(s) 186, 1140, 2389, 2527, 2923, 2953, 2975
MaeI CTAG 1 cut(s) 1263
MaeII ACGT 3 cut(s) 201, 338, 1219
MaeIII GTNAC 8 cut(s) 665, 1232, 1491, 2588, 2636, 2674, 2792, 2943
MalI GATC 6 cut(s) 611, 1160, 1659, 1715, 2748, 2973
MbiI CCGCTC 1 cut(s) 1248
MboI GATC 6 cut(s) 609, 1158, 1657, 1713, 2746, 2971
MflI RGATCY 2 cut(s) 609, 1657
MhlI GDGCHC 5 cut(s) 624, 801, 1008, 1758, 2832
MluCI AATT 9 cut(s) 462, 1140, 1410, 1626, 1648, 1726, 2669, 2702, 2939
MluI ACGCGT 1 cut(s) 870
MlyI GAGTC 9 cut(s) 116, 479, 683, 696, 907, 980, 1129, 1201, 1564
MmeI TCCRAC 8 cut(s) 115, 138, 351, 376, 423, 587, 1713, 2463
Mph1103I ATGCAT 2 cut(s) 2611, 2968
MroNI GCCGGC 1 cut(s) 970
MroXI GAANNNNTTC 3 cut(s) 953, 1428, 1457
MseI TTAA 4 cut(s) 632, 735, 2925, 2989
MslI CAYNNNNRTG 4 cut(s) 1524, 2433, 2457, 2979
MspA1I CMGCKG 4 cut(s) 919, 1045, 1069, 1971
MvaI CCWGG 3 cut(s) 139, 1655, 1865
MvnI CGCG 2 cut(s) 872, 1045
NaeI GCCGGC 1 cut(s) 972
NciI CCSGG 9 cut(s) 602, 603, 834, 1105, 1321, 1609, 1666, 1809, 2680
NdeI CATATG 1 cut(s) 1874
NdeII GATC 6 cut(s) 609, 1158, 1657, 1713, 2746, 2971
NgoMIV GCCGGC 1 cut(s) 970
NlaIV GGNNCC 6 cut(s) 388, 611, 894, 2195, 2500, 2831
NmeAIII GCCGAG 3 cut(s) 528, 1032, 1876
NmuCI GTSAC 3 cut(s) 1232, 2636, 2943
NsiI ATGCAT 2 cut(s) 2611, 2968
NspI RCATGY 5 cut(s) 186, 1286, 1387, 1639, 2317
PaeR7I CTCGAG 3 cut(s) 494, 641, 1295
PasI CCCWGGG 1 cut(s) 1864
PceI AGGCCT 2 cut(s) 81, 825
PciI ACATGT 2 cut(s) 1383, 1635
PcsI WCGNNNNNNNCGW 1 cut(s) 758
PdiI GCCGGC 1 cut(s) 972
PdmI GAANNNNTTC 3 cut(s) 953, 1428, 1457
PflFI GACNNNGTC 1 cut(s) 2765
PfoI TCCNGGA 4 cut(s) 137, 1103, 1607, 1664
PinAI ACCGGT 1 cut(s) 1239
PleI GAGTC 9 cut(s) 116, 478, 683, 696, 907, 980, 1128, 1200, 1563
PmaCI CACGTG 2 cut(s) 339, 1220
PmlI CACGTG 2 cut(s) 339, 1220
PpsI GAGTC 9 cut(s) 116, 478, 683, 696, 907, 980, 1128, 1200, 1563
Ppu21I YACGTR 2 cut(s) 339, 1220
PpuMI RGGWCCY 3 cut(s) 499, 646, 2815
PscI ACATGT 2 cut(s) 1383, 1635
PshAI GACNNNNGTC 1 cut(s) 1220
Psp124BI GAGCTC 4 cut(s) 624, 801, 1008, 1758
Psp5II RGGWCCY 3 cut(s) 499, 646, 2815
Psp6I CCWGG 3 cut(s) 137, 1653, 1863
PspCI CACGTG 2 cut(s) 339, 1220
PspEI GGTNACC 1 cut(s) 2674
PspFI CCCAGC 2 cut(s) 694, 2017
PspGI CCWGG 3 cut(s) 137, 1653, 1863
PspN4I GGNNCC 6 cut(s) 388, 611, 894, 2195, 2500, 2831
PspPPI RGGWCCY 3 cut(s) 499, 646, 2815
PspXI VCTCGAGB 3 cut(s) 494, 641, 1295
PstI CTGCAG 1 cut(s) 1437
PsuI RGATCY 2 cut(s) 609, 1657
PsyI GACNNNGTC 1 cut(s) 2765
PvuII CAGCTG 1 cut(s) 919
RsaI GTAC 7 cut(s) 1001, 1784, 1949, 2096, 2278, 2312, 2737
RsaNI GTAC 7 cut(s) 1000, 1783, 1948, 2095, 2277, 2311, 2736
RseI CAYNNNNRTG 4 cut(s) 1524, 2433, 2457, 2979
SacI GAGCTC 4 cut(s) 624, 801, 1008, 1758
SacII CCGCGG 1 cut(s) 1046
SalI GTCGAC 3 cut(s) 215, 699, 2222
SaqAI TTAA 4 cut(s) 632, 735, 2925, 2989
Sau3AI GATC 6 cut(s) 609, 1158, 1657, 1713, 2746, 2971
SchI GAGTC 9 cut(s) 116, 479, 683, 696, 907, 980, 1129, 1201, 1564
SduI GDGCHC 5 cut(s) 624, 801, 1008, 1758, 2832
SfaNI GCATC 7 cut(s) 186, 1140, 2389, 2527, 2923, 2953, 2975
SfcI CTRYAG 3 cut(s) 1433, 1977, 2644
Sfr274I CTCGAG 3 cut(s) 494, 641, 1295
Sfr303I CCGCGG 1 cut(s) 1046
SgrAI CRCCGGYG 1 cut(s) 1031
SgrBI CCGCGG 1 cut(s) 1046
SinI GGWCC 7 cut(s) 499, 646, 724, 1513, 2194, 2761, 2815
SlaI CTCGAG 3 cut(s) 494, 641, 1295
SmaI CCCGGG 1 cut(s) 603
SmiMI CAYNNNNRTG 4 cut(s) 1524, 2433, 2457, 2979
SmlI CTYRAG 3 cut(s) 494, 641, 1295
SmoI CTYRAG 3 cut(s) 494, 641, 1295
Sse9I AATT 9 cut(s) 462, 1140, 1410, 1626, 1648, 1726, 2669, 2702, 2939
SseBI AGGCCT 2 cut(s) 81, 825
SspI AATATT 1 cut(s) 2254
SspMI CTAG 1 cut(s) 1263
SstI GAGCTC 4 cut(s) 624, 801, 1008, 1758
StuI AGGCCT 2 cut(s) 81, 825
StyI CCWWGG 1 cut(s) 1908
TaaI ACNGT 3 cut(s) 1605, 2165, 2648
TaiI ACGT 3 cut(s) 204, 341, 1222
TaqII GACCGA 3 cut(s) 674, 785, 2557
TasI AATT 9 cut(s) 462, 1140, 1410, 1626, 1648, 1726, 2669, 2702, 2939
TatI WGTACW 4 cut(s) 1782, 1947, 2094, 2310
TauI GCSGC 5 cut(s) 267, 1045, 1069, 1282, 1352
Tru1I TTAA 4 cut(s) 632, 735, 2925, 2989
Tru9I TTAA 4 cut(s) 632, 735, 2925, 2989
TscAI CASTG 4 cut(s) 1468, 2119, 2136, 2622
TseFI GTSAC 3 cut(s) 1232, 2636, 2943
TseI GCWGC 5 cut(s) 862, 1039, 1276, 1904, 2402
Tsp45I GTSAC 3 cut(s) 1232, 2636, 2943
TspDTI ATGAA 8 cut(s) 17, 893, 1593, 1724, 1885, 2070, 2441, 2973
TspGWI ACGGA 2 cut(s) 202, 2921
TspMI CCCGGG 1 cut(s) 601
TspRI CASTG 4 cut(s) 1468, 2119, 2136, 2622
Tth111I GACNNNGTC 1 cut(s) 2765
VpaK11BI GGWCC 7 cut(s) 499, 646, 724, 1513, 2194, 2761, 2815
XapI RAATTY 2 cut(s) 462, 2702
XceI RCATGY 5 cut(s) 186, 1286, 1387, 1639, 2317
XhoI CTCGAG 3 cut(s) 494, 641, 1295
XmaI CCCGGG 1 cut(s) 601
XmiI GTMKAC 4 cut(s) 216, 513, 700, 2223
XmnI GAANNNNTTC 3 cut(s) 953, 1428, 1457
XspI CTAG 1 cut(s) 1263
Zsp2I ATGCAT 2 cut(s) 2611, 2968
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.