Rh5BG068700
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0012987
Physical Location & Seq
Forward (+)
5616517 .. 5621189
4673 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG068700.1

Sequence Viewer

Length: 3000 bp
ATGAAGACAAAAGTGCTTCTCTTCTTCCTCCTCCCTCTCCTCCTCTTCTCCTCCACCTCCTCCTCCTCCTCCCCCCTCACCCAAGAAGGCCTCTACCTCCAACACATCAAACACTCCCTCTCCGACCCCGACTCCTCCCTCTCCTCCTGGAACGACCAAGACGCCACCCCTTGCTCATGGGACGGCATCACATGCAACCCTTCCACCCACGTCATCACCTCCGTCGACCTCTCCAGCTTCAACCTCTTCGGCCCTTTCCCCTCCCTCCTCTGCCGCCTCCCCAACCTCACCTTCCTCTCCCTCTACAACAACTCCATCAACTCCACCCTACCCTCCGACATCTCCACGTGTCAGAACCTCCAACACCTCGACCTCTCCCAGAACCTCCTCACCGGCTCCCTCCCCTCCACCCTCCCCGACCTCCCCAACCTCCGCTACCTCGACCTCACCGGCAACAACTTCTCGGGAGAAATTCCCGAGTCTTTCGGGAGATTCCAAAAACTCGAGGTCCTCTCTCTCGTCTACAACCTCCTCGACAGCGCCCTGCCCCCATTTCTCGGCAATATCTCGTCCCTCAAAATGCTCAACCTTTCTTACAACCCGTTCTTCCCGGGTCGGATCCCGCCCGAGCTCGGAAACTTAACGAACCTCGAGGTCCTCTGGCTGACCGAGTGTAACTTAGTCGGCGAAATACCCGACTCGCTGGGTCGACTCAGTAAACTCACCGATTTGGACCTCGCCATTAACGCCCTGCACGGCCCGATTCCGGCTTCGTTGACGGAGTTGACCAGCGTGGTTCAGATTGAGCTCTATAACAACACCTTGACCGGAGCCCTGCCGCCGGGAATGTCGAAGCTGAAGAAGCTCCGGCTGCTCGACGCGTCCATGAACCGGCTGACGGGGCCGATTCCCGACGAGTTGATTCGGCTGGAGCTGGAAAGCCTGAATCTTTACGAGAACAACTTCGAGGGGAGTTTGCCGGCGACCATTGCCGACTCGCCGAACCTGTACGAGCTCCGGCTGTTTCAGAACAAGCTCACCGGCGAGCTGCCGCGGAATCTCGGCCAGAACTCGCCGCTGAGGTGGATAGACCTGTCGAACAACCAGTTTTCCGGGAGCATTCCGGCGAGTCTGTGCGAAAAGGGGCAAATTGAGGAGATGCTGATGATCAACAATGCTTTTTCCGGCGAGATTCCGGCGAGTCTCGGGGAGTGTCAGAGCTTGACACGTGTCCGGTTGGGTCACAACCGGTTGAGCGGTGAAGTTCCAGCGGCTTTCTGGGGGCTGCCGCATGTCTATTTGCTCGAGCTGGTTGATAACCAGTTATCGGGGCCGATAGCCAAGACAATTGCCGGTGCGGCTAACCTTTCGCTTCTGATAATCGAAAAGAACCAGTTTGAAGGTCCGATACCGGAGGAAATTGGGTGGGTTGAAAATCTTCTGCAGTTTTCCGGCGGTGAGAATAGGTTCAGTGGGCCTTTGCCGGAGACAATAGTTAAGTTACGACATCTGAGCACGCTGGACCTTCACAACAATGAGCTTTCCGGTGAGTTTCCGAGTGGGAGTCAGTCTTTGGTTCGGCTCAATGAGCTCAATTTGAGGAATAACCAACTGTCCGGGAAAATCCCAGATGGAATTGGGAACATGTCTTCGCTGAATTACCTTGATCTCTCCGGGAATCGGTTTTCGGGTCAAGTTCCGGTTTGGTTGCAGAATATGAAGATCAACGTGTTCAATTTGTCGGACAACCAGCTTTCGGGTGAGCTCCCTCCTTTGTTTGCGAAGGAGATGTACAGAAATAGCTTTCTGGGAAATCCGGGTCTTTGTGGAGATTTGGAGGGGTTGTGTGAGAGCAGAGCCGAGGAGAAGACCCAGGGCTACATATGGTTGCTGAGGTGCATTTTCATTCTGGCTGCCTTGGTGTTTGTGGTGGGTGTGGTTTGGTTTTACTTCAAGTACAAGAACTTCAAGAAGGCCAGCGGTGCTATAGATAAATCCAAGTGGACTTTGATGTCGTTTCACAAGCTGGGTTTCAGCGAGTATGAGATACTGGATTGCCTTGATGAAGATAATGTGATTGGGACTGGGGGGTCTGGGAAAGTGTACAAGGTTGAGCTGAGCAATAAGGAGGTTGTTGCAGTGAAAAAGCTGTGGAGGGGAAAAGTGAAAGAGTGTGAAAGCGATGATGTTGAGAAAGGTTGGGTCCAAGATGATGGCTTTGAGGCGGAGGTCGACACTTTGGGGAGGATTAGGCACAAGAACATTGTGAAGCTTTGGTGCTGTTGTACCGCAAGAGATTGCAAGCTTTTGGTGTATGAGTACATGCCAAATGGTAGTCTTGGTGATTTGCTGCATAGTAGCAAAGCAGGGTTGCTGGATTGGCCTACAAGGTTCAAGATTGCTCTTGATGCTGCTGATGGGCTTTCGTATCTGCACCATGATTGTGTTCCTGCTATTGTTCATAGAGATGTGAAATCAAACAATATATTGTTGGATGGGGATTTCGGAGCCAGAGTAGCGGATTTTGGAGTAGCTAAGGTCGTTGATGCAACTGGAAAAGGGGCTAAATCCATGTCGGTCATTGCAGGTTCTTGTGGTTACATTGCTCCAGAATATGCATACACACTGAGGGTGAATGAGAAGAGTGACATCTACAGTTTTGGTGTGGTGATTCTGGAATTGGTTACCGGGAAACTTCCAGTTGACCCAGAATTCGGAGAAAAGGACTTGGTGAAGTGGGTCTGTACCACCTTAGATCAGAAAGGAGTGGACCATGTCATTGACCCCAAACTCGATTCTTGTTACAAGGAAGAAGTATGCAAGGTCCTCAATATCGGGCTCCTCTGCACCAGTCCTCTCCCAATCAATAGACCATCGATGAGAAGAGTGGTGAAGTTGTTGCAAGAAGCGGGAACGGAGAAGCATCCTCAGATTAAGAAGGAAGGCAAATTGTCACCATATTACTATGAAGATGCATCGGATCATGGAAGTGTAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

999

Amino Acids

110.36

Weight (kDa)

5.24

Isoelectric Point (pI)

37.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 66 1.1e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 90 - 175 7.5e-08 Leucine-rich repeat region
LRR_8 PF13855 94 - 154 9.3e-09 Leucine rich repeat
LRR_4 PF12799 118 - 158 2.5e-08 Leucine Rich repeats (2 copies)
LRR_14 PF23598 186 - 299 1.5e-08 Leucine-rich repeat region
LRR_14 PF23598 493 - 558 1.5e-08 Leucine-rich repeat region
LRR_8 PF13855 502 - 562 2.8e-08 Leucine rich repeat
Pkinase PF00069 690 - 915 8.6e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 690 - 965 6.6e-36 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012987)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28440
fragaria_vesca FvH4_3g08530 FvH4_3g08530
malus_domestica MD05G1309900.v1.1 MD10G1288500.v1.1
prunus_persica Prupe.4G053100_v2.0.a1
pyrus_communis pycom05g28630
rosa_chinensis RchiOBHm_Chr5g0009311
rosa_laevigata RLG00000031647
rosa_multiflora Rmu_sc0006233.1_g000007
rosa_roxburghii Rroxscaffold_1G00066780 Rroxscaffold_1G00067480
rosa_rugosa Rorug04G0441700
rosa_samantha Rh5AG072200 Rh5BG068700 Rh5CG081100 Rh5DG068900
rosa_wichuraiana Rw5G006750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 2011, 2089
AbsI CCTCGAGG 1 cut(s) 650
Acc36I ACCTGC 1 cut(s) 2576
AccBSI CCGCTC 1 cut(s) 1257
AccI GTMKAC 4 cut(s) 225, 522, 709, 2232
AccII CGCG 2 cut(s) 881, 1054
AclWI GGATC 3 cut(s) 613, 626, 2988
AcoI YGGCCR 1 cut(s) 1063
AcsI RAATTY 2 cut(s) 471, 2711
AcuI CTGAAG 1 cut(s) 878
AcvI CACGTG 2 cut(s) 348, 1229
AcyI GRCGYC 1 cut(s) 162
AfaI GTAC 7 cut(s) 1010, 1793, 1958, 2105, 2287, 2321, 2746
AflIII ACRYGT 6 cut(s) 347, 879, 1226, 1228, 1644, 1728
AgeI ACCGGT 1 cut(s) 1248
AgsI TTSAA 7 cut(s) 241, 1400, 1433, 1735, 1954, 1969, 2395
AjiI CACGTC 1 cut(s) 211
AjnI CCWGG 2 cut(s) 146, 1872
AjuI GAANNNNNNNTTGG 2 cut(s) 275, 307
Alw21I GWGCWC 6 cut(s) 633, 810, 1017, 1517, 1593, 1767
Alw26I GTCTC 2 cut(s) 1208, 1481
AlwI GGATC 3 cut(s) 613, 626, 2988
Ama87I CYCGRG 8 cut(s) 463, 476, 503, 610, 626, 650, 1205, 1304
AoxI GGCC 9 cut(s) 88, 250, 757, 902, 1063, 1331, 1475, 1974, 2381
ApeKI GCWGC 6 cut(s) 871, 1048, 1285, 1913, 2350, 2411
ApoI RAATTY 2 cut(s) 471, 2711
ArsI GACNNNNNNTTYG 2 cut(s) 2696, 2728
AsiGI ACCGGT 1 cut(s) 1248
Asp700I GAANNNNTTC 3 cut(s) 962, 1437, 1466
AspLEI GCGC 1 cut(s) 542
AsuC2I CCSGG 8 cut(s) 611, 612, 843, 1114, 1618, 1675, 1818, 2689
AvaI CYCGRG 8 cut(s) 463, 476, 503, 610, 626, 650, 1205, 1304
AvaII GGWCC 8 cut(s) 508, 655, 733, 1403, 1522, 2203, 2770, 2824
BamHI GGATCC 1 cut(s) 618
BanII GRGCYC 7 cut(s) 633, 810, 835, 1017, 1593, 1767, 2841
BarI GAAGNNNNNNTAC 6 cut(s) 1392, 1424, 1775, 1807, 1949, 1981
BbrPI CACGTG 2 cut(s) 348, 1229
BbsI GAAGAC 3 cut(s) 11, 1641, 1874
Bbv12I GWGCWC 6 cut(s) 633, 810, 1017, 1517, 1593, 1767
BbvCI CCTCAGC 2 cut(s) 1079, 1892
BbvI GCAGC 6 cut(s) 858, 1035, 1272, 1900, 2337, 2398
BccI CCATC 6 cut(s) 323, 1625, 2207, 2411, 2489, 2881
BceAI ACGGC 2 cut(s) 199, 772
BciT130I CCWGG 2 cut(s) 148, 1874
BclI TGATCA 1 cut(s) 1167
BcnI CCSGG 8 cut(s) 611, 612, 843, 1114, 1618, 1675, 1818, 2689
BcoDI GTCTC 2 cut(s) 1208, 1481
BfmI CTRYAG 3 cut(s) 1442, 1986, 2653
BfoI RGCGCY 1 cut(s) 543
BfuAI ACCTGC 1 cut(s) 2576
BglI GCCNNNNNGGC 1 cut(s) 1358
BlpI GCTNAGC 1 cut(s) 2117
Bme18I GGWCC 8 cut(s) 508, 655, 733, 1403, 1522, 2203, 2770, 2824
BmeT110I CYCGRG 8 cut(s) 463, 476, 503, 610, 626, 650, 1205, 1304
BmgBI CACGTC 1 cut(s) 211
BmiI GGNNCC 8 cut(s) 397, 620, 832, 903, 1332, 2204, 2509, 2840
BmrI ACTGGG 1 cut(s) 2094
BmsI GCATC 7 cut(s) 195, 1149, 2398, 2536, 2932, 2962, 2984
BmuI ACTGGG 1 cut(s) 2094
BoxI GACNNNNGTC 1 cut(s) 1229
BpiI GAAGAC 3 cut(s) 11, 1641, 1874
BplI GAGNNNNNCTC 2 cut(s) 497, 529
BpmI CTGGAG 3 cut(s) 217, 950, 2592
Bpu10I CCTNAGC 3 cut(s) 1079, 1892, 2535
Bpu1102I GCTNAGC 1 cut(s) 2117
BpuMI CCSGG 8 cut(s) 611, 612, 843, 1114, 1618, 1675, 1818, 2689
Bsa29I ATCGAT 1 cut(s) 2876
BsaAI YACGTR 2 cut(s) 348, 1229
BsaHI GRCGYC 1 cut(s) 162
BsaJI CCNNGG 6 cut(s) 610, 1052, 1860, 1872, 1873, 1917
BsaWI WCCGGW 6 cut(s) 827, 1233, 1248, 1411, 1544, 1699
Bse118I RCCGGY 7 cut(s) 392, 449, 891, 979, 1040, 1248, 1352
Bse1I ACTGG 8 cut(s) 1105, 1321, 1393, 2055, 2089, 2557, 2699, 2850
Bse3DI GCAATG 3 cut(s) 987, 2580, 2601
BseBI CCWGG 2 cut(s) 148, 1874
BseCI ATCGAT 1 cut(s) 2876
BseDI CCNNGG 6 cut(s) 610, 1052, 1860, 1872, 1873, 1917
BseGI GGATG 2 cut(s) 2500, 2923
BseMI GCAATG 3 cut(s) 987, 2580, 2601
BseMII CTCAG 7 cut(s) 727, 1070, 1502, 1883, 2108, 2618, 2942
BseNI ACTGG 8 cut(s) 1105, 1321, 1393, 2055, 2089, 2557, 2699, 2850
BseXI GCAGC 6 cut(s) 858, 1035, 1272, 1900, 2337, 2398
BseYI CCCAGC 2 cut(s) 703, 2026
BsgI GTGCAG 3 cut(s) 737, 2417, 2830
Bsh1236I CGCG 2 cut(s) 881, 1054
BshFI GGCC 9 cut(s) 90, 252, 759, 904, 1065, 1333, 1477, 1976, 2383
BshTI ACCGGT 1 cut(s) 1248
BshVI ATCGAT 1 cut(s) 2876
BsiHKAI GWGCWC 6 cut(s) 633, 810, 1017, 1517, 1593, 1767
BsiHKCI CYCGRG 8 cut(s) 463, 476, 503, 610, 626, 650, 1205, 1304
BslFI GGGAC 3 cut(s) 194, 556, 2095
BsmAI GTCTC 2 cut(s) 1208, 1481
BsmFI GGGAC 3 cut(s) 194, 556, 2095
BsmI GAATGC 1 cut(s) 1119
BsnI GGCC 9 cut(s) 90, 252, 759, 904, 1065, 1333, 1477, 1976, 2383
BsoBI CYCGRG 8 cut(s) 463, 476, 503, 610, 626, 650, 1205, 1304
Bsp1286I GDGCHC 8 cut(s) 633, 810, 835, 1017, 1517, 1593, 1767, 2841
Bsp1407I TGTACA 2 cut(s) 1791, 2103
Bsp143I GATC 6 cut(s) 618, 1167, 1666, 1722, 2755, 2980
Bsp1720I GCTNAGC 1 cut(s) 2117
BspANI GGCC 9 cut(s) 90, 252, 759, 904, 1065, 1333, 1477, 1976, 2383
BspCNI CTCAG 7 cut(s) 726, 1071, 1503, 1884, 2109, 2619, 2941
BspDI ATCGAT 1 cut(s) 2876
BspFNI CGCG 2 cut(s) 881, 1054
BspLI GGNNCC 8 cut(s) 397, 620, 832, 903, 1332, 2204, 2509, 2840
BspMAI CTGCAG 1 cut(s) 1446
BspMI ACCTGC 1 cut(s) 2576
BspPI GGATC 3 cut(s) 613, 626, 2988
BsrBI CCGCTC 1 cut(s) 1257
BsrDI GCAATG 3 cut(s) 987, 2580, 2601
BsrFI RCCGGY 7 cut(s) 392, 449, 891, 979, 1040, 1248, 1352
BsrGI TGTACA 2 cut(s) 1791, 2103
BsrI ACTGG 8 cut(s) 1105, 1321, 1393, 2055, 2089, 2557, 2699, 2850
BssAI RCCGGY 7 cut(s) 392, 449, 891, 979, 1040, 1248, 1352
BssECI CCNNGG 6 cut(s) 610, 1052, 1860, 1872, 1873, 1917
BssMI GATC 6 cut(s) 618, 1167, 1666, 1722, 2755, 2980
BssNI GRCGYC 1 cut(s) 162
BssT1I CCWWGG 1 cut(s) 1917
Bst2UI CCWGG 2 cut(s) 148, 1874
Bst4CI ACNGT 2 cut(s) 1614, 2657
Bst6I CTCTTC 5 cut(s) 26, 50, 251, 2636, 2878
BstACI GRCGYC 1 cut(s) 162
BstAPI GCANNNNNTGC 1 cut(s) 192
BstAUI TGTACA 2 cut(s) 1791, 2103
BstBAI YACGTR 2 cut(s) 348, 1229
BstC8I GCNNGC 5 cut(s) 981, 1046, 1517, 1978, 2303
BstDSI CCRYGG 1 cut(s) 1052
BstEII GGTNACC 1 cut(s) 2683
BstF5I GGATG 2 cut(s) 2500, 2923
BstFNI CGCG 2 cut(s) 881, 1054
BstH2I RGCGCY 1 cut(s) 543
BstHHI GCGC 1 cut(s) 542
BstKTI GATC 6 cut(s) 621, 1170, 1669, 1725, 2758, 2983
BstMAI GTCTC 2 cut(s) 1208, 1481
BstMBI GATC 6 cut(s) 618, 1167, 1666, 1722, 2755, 2980
BstNI CCWGG 2 cut(s) 148, 1874
BstNSI RCATGY 4 cut(s) 195, 1295, 1648, 2326
BstPAI GACNNNNGTC 1 cut(s) 1229
BstPI GGTNACC 1 cut(s) 2683
BstSFI CTRYAG 3 cut(s) 1442, 1986, 2653
BstUI CGCG 2 cut(s) 881, 1054
BstV1I GCAGC 6 cut(s) 858, 1035, 1272, 1900, 2337, 2398
BstV2I GAAGAC 3 cut(s) 11, 1641, 1874
BstX2I RGATCY 1 cut(s) 618
BstXI CCANNNNNNTGG 1 cut(s) 2213
BstYI RGATCY 1 cut(s) 618
Bsu15I ATCGAT 1 cut(s) 2876
BsuRI GGCC 9 cut(s) 90, 252, 759, 904, 1065, 1333, 1477, 1976, 2383
BsuTUI ATCGAT 1 cut(s) 2876
BtgI CCRYGG 1 cut(s) 1052
BtgZI GCGATG 1 cut(s) 2196
BtrI CACGTC 1 cut(s) 211
BtsCI GGATG 2 cut(s) 2500, 2923
BtsI GCAGTG 1 cut(s) 2145
BtsIMutI CAGTG 3 cut(s) 1477, 2145, 2624
BveI ACCTGC 1 cut(s) 2576
Cac8I GCNNGC 5 cut(s) 981, 1046, 1517, 1978, 2303
CfoI GCGC 1 cut(s) 542
Cfr10I RCCGGY 7 cut(s) 392, 449, 891, 979, 1040, 1248, 1352
Cfr42I CCGCGG 1 cut(s) 1055
Cfr9I CCCGGG 1 cut(s) 610
ClaI ATCGAT 1 cut(s) 2876
CseI GACGC 3 cut(s) 170, 870, 887
Csp6I GTAC 7 cut(s) 1009, 1792, 1957, 2104, 2286, 2320, 2745
CspAI ACCGGT 1 cut(s) 1248
CviQI GTAC 7 cut(s) 1009, 1792, 1957, 2104, 2286, 2320, 2745
DpnI GATC 6 cut(s) 620, 1169, 1668, 1724, 2757, 2982
DpnII GATC 6 cut(s) 618, 1167, 1666, 1722, 2755, 2980
DrdI GACNNNNNNGTC 2 cut(s) 2011, 2089
DseDI GACNNNNNNGTC 2 cut(s) 2011, 2089
EaeI YGGCCR 1 cut(s) 1063
Eam1104I CTCTTC 5 cut(s) 26, 50, 251, 2636, 2878
EarI CTCTTC 5 cut(s) 26, 50, 251, 2636, 2878
EciI GGCGGA 1 cut(s) 2240
Ecl136II GAGCTC 5 cut(s) 631, 808, 1015, 1591, 1765
Eco130I CCWWGG 1 cut(s) 1917
Eco147I AGGCCT 1 cut(s) 90
Eco24I GRGCYC 7 cut(s) 633, 810, 835, 1017, 1593, 1767, 2841
Eco47I GGWCC 8 cut(s) 508, 655, 733, 1403, 1522, 2203, 2770, 2824
Eco53kI GAGCTC 5 cut(s) 631, 808, 1015, 1591, 1765
Eco57I CTGAAG 1 cut(s) 878
Eco72I CACGTG 2 cut(s) 348, 1229
Eco88I CYCGRG 8 cut(s) 463, 476, 503, 610, 626, 650, 1205, 1304
Eco91I GGTNACC 1 cut(s) 2683
EcoICRI GAGCTC 5 cut(s) 631, 808, 1015, 1591, 1765
EcoO109I RGGNCCY 3 cut(s) 508, 655, 2824
EcoO65I GGTNACC 1 cut(s) 2683
EcoRI GAATTC 1 cut(s) 2711
EcoRII CCWGG 2 cut(s) 146, 1872
EcoT14I CCWWGG 1 cut(s) 1917
EcoT22I ATGCAT 2 cut(s) 2620, 2977
EcoT38I GRGCYC 7 cut(s) 633, 810, 835, 1017, 1593, 1767, 2841
ErhI CCWWGG 1 cut(s) 1917
FalI AAGNNNNNCTT 1 cut(s) 2980
FaqI GGGAC 3 cut(s) 194, 556, 2095
FauI CCCGC 2 cut(s) 630, 2902
FauNDI CATATG 1 cut(s) 1883
FbaI TGATCA 1 cut(s) 1167
FblI GTMKAC 4 cut(s) 225, 522, 709, 2232
FokI GGATG 2 cut(s) 2507, 2910
FriOI GRGCYC 7 cut(s) 633, 810, 835, 1017, 1593, 1767, 2841
GlaI GCGC 1 cut(s) 541
GsaI CCCAGC 2 cut(s) 707, 2030
GsuI CTGGAG 3 cut(s) 217, 950, 2592
HaeII RGCGCY 1 cut(s) 543
HaeIII GGCC 9 cut(s) 90, 252, 759, 904, 1065, 1333, 1477, 1976, 2383
HgaI GACGC 3 cut(s) 170, 870, 887
HhaI GCGC 1 cut(s) 542
Hin1I GRCGYC 1 cut(s) 162
Hin6I GCGC 1 cut(s) 540
HinP1I GCGC 1 cut(s) 540
HincII GTYRAC 6 cut(s) 226, 710, 777, 786, 2233, 2704
HindII GTYRAC 6 cut(s) 226, 710, 777, 786, 2233, 2704
HindIII AAGCTT 2 cut(s) 2270, 2303
Hpy188III TCNNGA 9 cut(s) 465, 476, 487, 911, 1969, 2395, 2405, 2609, 2675
Hpy99I CGWCG 3 cut(s) 227, 881, 917
HpyAV CCTTC 9 cut(s) 80, 210, 301, 1394, 1535, 1777, 1966, 2932, 2936
HpyCH4III ACNGT 2 cut(s) 1614, 2657
HpyCH4IV ACGT 4 cut(s) 210, 347, 1228, 1728
HpySE526I ACGT 4 cut(s) 210, 347, 1228, 1728
Hsp92I GRCGYC 1 cut(s) 162
HspAI GCGC 1 cut(s) 540
KroI GCCGGC 1 cut(s) 979
KroNI GCCGGC 1 cut(s) 981
Ksp22I TGATCA 1 cut(s) 1167
KspI CCGCGG 1 cut(s) 1055
Kzo9I GATC 6 cut(s) 618, 1167, 1666, 1722, 2755, 2980
Lsp1109I GCAGC 6 cut(s) 858, 1035, 1272, 1900, 2337, 2398
LweI GCATC 7 cut(s) 195, 1149, 2398, 2536, 2932, 2962, 2984
MaeII ACGT 4 cut(s) 210, 347, 1228, 1728
MaeIII GTNAC 8 cut(s) 674, 1241, 1500, 2597, 2645, 2683, 2801, 2952
MalI GATC 6 cut(s) 620, 1169, 1668, 1724, 2757, 2982
MbiI CCGCTC 1 cut(s) 1257
MboI GATC 6 cut(s) 618, 1167, 1666, 1722, 2755, 2980
MfeI CAATTG 1 cut(s) 1347
MflI RGATCY 1 cut(s) 618
MhlI GDGCHC 8 cut(s) 633, 810, 835, 1017, 1517, 1593, 1767, 2841
MluI ACGCGT 1 cut(s) 879
MlyI GAGTC 8 cut(s) 125, 488, 692, 705, 989, 1138, 1210, 1573
MmeI TCCRAC 7 cut(s) 124, 147, 360, 385, 596, 1722, 2472
Mph1103I ATGCAT 2 cut(s) 2620, 2977
MroNI GCCGGC 1 cut(s) 979
MroXI GAANNNNTTC 3 cut(s) 962, 1437, 1466
MseI TTAA 5 cut(s) 641, 744, 1497, 2934, 2998
MslI CAYNNNNRTG 4 cut(s) 1533, 2442, 2466, 2988
MspA1I CMGCKG 4 cut(s) 1054, 1078, 1271, 1980
MunI CAATTG 1 cut(s) 1347
Mva1269I GAATGC 1 cut(s) 1119
MvaI CCWGG 2 cut(s) 148, 1874
MvnI CGCG 2 cut(s) 881, 1054
NaeI GCCGGC 1 cut(s) 981
NciI CCSGG 8 cut(s) 611, 612, 843, 1114, 1618, 1675, 1818, 2689
NdeI CATATG 1 cut(s) 1883
NdeII GATC 6 cut(s) 618, 1167, 1666, 1722, 2755, 2980
NgoMIV GCCGGC 1 cut(s) 979
NlaIV GGNNCC 8 cut(s) 397, 620, 832, 903, 1332, 2204, 2509, 2840
NmeAIII GCCGAG 3 cut(s) 537, 1041, 1885
NmuCI GTSAC 3 cut(s) 1241, 2645, 2952
NsiI ATGCAT 2 cut(s) 2620, 2977
NspI RCATGY 4 cut(s) 195, 1295, 1648, 2326
PaeR7I CTCGAG 3 cut(s) 503, 650, 1304
PasI CCCWGGG 1 cut(s) 1873
PceI AGGCCT 1 cut(s) 90
PciI ACATGT 1 cut(s) 1644
PctI GAATGC 1 cut(s) 1119
PdiI GCCGGC 1 cut(s) 981
PdmI GAANNNNTTC 3 cut(s) 962, 1437, 1466
PflFI GACNNNGTC 1 cut(s) 2774
PfoI TCCNGGA 4 cut(s) 146, 1112, 1616, 1673
PinAI ACCGGT 1 cut(s) 1248
PleI GAGTC 8 cut(s) 125, 487, 692, 705, 989, 1137, 1209, 1572
PmaCI CACGTG 2 cut(s) 348, 1229
PmlI CACGTG 2 cut(s) 348, 1229
PpsI GAGTC 8 cut(s) 125, 487, 692, 705, 989, 1137, 1209, 1572
Ppu21I YACGTR 2 cut(s) 348, 1229
PpuMI RGGWCCY 3 cut(s) 508, 655, 2824
PscI ACATGT 1 cut(s) 1644
PshAI GACNNNNGTC 1 cut(s) 1229
Psp124BI GAGCTC 5 cut(s) 633, 810, 1017, 1593, 1767
Psp5II RGGWCCY 3 cut(s) 508, 655, 2824
Psp6I CCWGG 2 cut(s) 146, 1872
PspCI CACGTG 2 cut(s) 348, 1229
PspEI GGTNACC 1 cut(s) 2683
PspFI CCCAGC 2 cut(s) 703, 2026
PspGI CCWGG 2 cut(s) 146, 1872
PspN4I GGNNCC 8 cut(s) 397, 620, 832, 903, 1332, 2204, 2509, 2840
PspPPI RGGWCCY 3 cut(s) 508, 655, 2824
PspXI VCTCGAGB 3 cut(s) 503, 650, 1304
PstI CTGCAG 1 cut(s) 1446
PsuI RGATCY 1 cut(s) 618
PsyI GACNNNGTC 1 cut(s) 2774
RsaI GTAC 7 cut(s) 1010, 1793, 1958, 2105, 2287, 2321, 2746
RsaNI GTAC 7 cut(s) 1009, 1792, 1957, 2104, 2286, 2320, 2745
RseI CAYNNNNRTG 4 cut(s) 1533, 2442, 2466, 2988
SacI GAGCTC 5 cut(s) 633, 810, 1017, 1593, 1767
SacII CCGCGG 1 cut(s) 1055
SalI GTCGAC 3 cut(s) 224, 708, 2231
SaqAI TTAA 5 cut(s) 641, 744, 1497, 2934, 2998
Sau3AI GATC 6 cut(s) 618, 1167, 1666, 1722, 2755, 2980
SchI GAGTC 8 cut(s) 125, 488, 692, 705, 989, 1138, 1210, 1573
SduI GDGCHC 8 cut(s) 633, 810, 835, 1017, 1517, 1593, 1767, 2841
SfaNI GCATC 7 cut(s) 195, 1149, 2398, 2536, 2932, 2962, 2984
SfcI CTRYAG 3 cut(s) 1442, 1986, 2653
Sfr274I CTCGAG 3 cut(s) 503, 650, 1304
Sfr303I CCGCGG 1 cut(s) 1055
SgrAI CRCCGGYG 1 cut(s) 1040
SgrBI CCGCGG 1 cut(s) 1055
SinI GGWCC 8 cut(s) 508, 655, 733, 1403, 1522, 2203, 2770, 2824
SlaI CTCGAG 3 cut(s) 503, 650, 1304
SmaI CCCGGG 1 cut(s) 612
SmiMI CAYNNNNRTG 4 cut(s) 1533, 2442, 2466, 2988
SmlI CTYRAG 3 cut(s) 503, 650, 1304
SmoI CTYRAG 3 cut(s) 503, 650, 1304
SseBI AGGCCT 1 cut(s) 90
SstI GAGCTC 5 cut(s) 633, 810, 1017, 1593, 1767
StuI AGGCCT 1 cut(s) 90
StyI CCWWGG 1 cut(s) 1917
TaaI ACNGT 2 cut(s) 1614, 2657
TaiI ACGT 4 cut(s) 213, 350, 1231, 1731
TaqII GACCGA 2 cut(s) 683, 2566
TatI WGTACW 4 cut(s) 1791, 1956, 2103, 2319
TauI GCSGC 7 cut(s) 276, 841, 1054, 1078, 1274, 1291, 1361
Tru1I TTAA 5 cut(s) 641, 744, 1497, 2934, 2998
Tru9I TTAA 5 cut(s) 641, 744, 1497, 2934, 2998
TscAI CASTG 3 cut(s) 1477, 2145, 2631
TseFI GTSAC 3 cut(s) 1241, 2645, 2952
TseI GCWGC 6 cut(s) 871, 1048, 1285, 1913, 2350, 2411
Tsp45I GTSAC 3 cut(s) 1241, 2645, 2952
TspDTI ATGAA 7 cut(s) 17, 902, 1733, 1894, 2079, 2450, 2982
TspGWI ACGGA 3 cut(s) 211, 794, 2930
TspMI CCCGGG 1 cut(s) 610
TspRI CASTG 3 cut(s) 1477, 2145, 2631
Tth111I GACNNNGTC 1 cut(s) 2774
VpaK11BI GGWCC 8 cut(s) 508, 655, 733, 1403, 1522, 2203, 2770, 2824
XapI RAATTY 2 cut(s) 471, 2711
XceI RCATGY 4 cut(s) 195, 1295, 1648, 2326
XcmI CCANNNNNNNNNTGG 1 cut(s) 1275
XhoI CTCGAG 3 cut(s) 503, 650, 1304
XmaI CCCGGG 1 cut(s) 610
XmiI GTMKAC 4 cut(s) 225, 522, 709, 2232
XmnI GAANNNNTTC 3 cut(s) 962, 1437, 1466
Zsp2I ATGCAT 2 cut(s) 2620, 2977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.