Rroxscaffold_1G00067480
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0012987
Physical Location & Seq
Reverse (-)
88784181 .. 88787779
3599 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00067480.1

Sequence Viewer

Length: 2601 bp
ATGAAGACAAAAGTGATTCTCTTCTTCCTCCTCCCTCTCCTCCTCTTCTCCTCCACCTCCTCCTCCCTCACCCAAGAAGGCCTCTACCTCCAACACATCAAACACTCCCTCTCCGACCCCGACTCCTCCCTCTCCTCCTGGAACGACCAAGACGCCACCCCTTGCTTATGGGACGGCATCACATGCAACCCTTCCACCCACGTCATCACCTCCGTCGACCTCTCCGGCTTCAACCTCTTCGGCCCTTTCCCCTCCCTCTTCTGCCGCCTCCCCAGCCTCACCTTCCTCTCCCTCTACAACAACTCCATCAACTCCACCCTACCCTCCGACATCTCCACGTGTCAGAACCTCCAACACCTCGACCTCTCCCAGAACCTCCTCACCGGCTCCCTCCCCTCCACCCTCGCCGACCTCCCCAACCTCCGCTACCTCGACCTCACCGGCAACAACTTCTCGGGAGAAATTCCCGAGTCTTTCGGGAGATTCCAAAAACTCGAGGTCCTCTCTCTCGTCTACAACCTCCTCGACAGCGCCCTGCCCCCATTTCTCGGCAATATCTCGTCCCTCAAAATGCTCAACCTTTCTTACAACCCGTTTTTCCCGGGTCGGATCCCGCCCGAGCTCGGAAACTTAACGAACCTCGAGGTCCTCTGGCTGACCGAGTGTAACTTAGTCGGCGAAATACCCGACTCGCTGGGTCGACTCAGTAAACTCACCGATTTGGACCTCGCCATTAACGCCCTGCACGGCCCGATTCCGGCGTCGTTGACCCGGTTGACCAGCGTGGTTCAGATTGAGCTCTATAACAACACCTTGACCGGAGGCCTGCCGCCGGGAATGTCGAAGCTGAAGAATCTCCGGCTGCTCGACGCGTCCATGAACCGGCTGACGGGGCCGATTCCCGACGATTTGACTCGGCTGGAGCTGGAAAGCCTGAATCTTTACGAGAACAACTTCGAGGGGAGTTTGCCGGCGAGCATTGCCGACTCGCCTAACCTGTACGAGCTCCGGCTGTTTCAGAACAAGCTCACCGGCGAGCTGCCGCGGAATCTCGGCCAGAACTCGCCGCTGAGGTGGATAGACCTGTCGAACAACCAGTTTTCCGGGAGCATTCCGGCGAGTCTGTGCGAAAAGGGGCAAATTGAGGAGATGCTGATGATCAACAATGCTTTTTCCGGCCAGATTCCGGCGAGTCTCGGGGAGTGTCAGAGCTTGACACGTGTCCGGCTGGGTCACAACCGGTTGAGCGGGGAAGTTCCGGCTAGTTTCTGGGGGCTGCCGCATGTCTATTTGCTGGAGCTGGTTGATAACCAGTTGTCCGGGCCGATAGGCAAGACTATTGCCGGTGCGGCTAACCTTTCGCTTCTGATAATCGAAAAGAACCAGTTTGAAGGTTCGATACCGGAGGAAATTGGGTGGGTTGAAAATCTTCTGCAGTTTTCCGGCGGCGAGAATAGGTTCAGTGGGCCTCTGCCGGAGACAATAGTTAAGTTACGACATCTTAGCACGCTGGACCTTCACAACAATGAGCTTTCCGGTGCGTTTCCGAGTGGGAGTCAGTCTTTGGTTCGGCTCAATGAGCTCAATTTGAAGAATAACCAACTGTCCGGGAAAATCCCAGATGGAATTGGGAACATGTCTTCGCTGAATTACCTTGACCTCTCCGGGAATCGGTTTTCGGGTCAAGTTCCGGTTTGGTTGCAGAATATGAGGATCAATGTGTTCAATTTGTCGGACAACCAGCTTTCGGGTGAGCTCCCTCCTTTGTTTGCCAAGGACATGTACAGAAATAGCTTTCTGGGAAATCCGGGTCTTTGTGGAGATTTGGAGGGGTTGTGTGAGAGCAGAGCCGAGGAGAAAACCCAGGGCTACATATGGTTGCTGAGGTGCATTTTCATTCTGGCTGCCTTGGTGTTTGTGGTGGGTGTGGTTTGGTTTTACTTCAAGTACAAGAACTTCAAGAAGGCCAGCGGTGCTATAGATAAATCCAAGTGGACTTTGATGTCGTTTCACAAGCTGGGTTTCAGCGAGTATGAGATACTGGATTGCCTTGATGAAGATAATGTGATTGGGACTGGGGGGTCTGGGAAAGTGTACAAGGTTGTGCTGAGCAATGGTGAGGTTGTTGCAGTGAAAAAGCTGTGGAGAGGGAAAGTGAAAGAGTGTGAAAGCGATGATGTTGAGAAAGGTTCTTGTGGTTACATTGCTCCAGAATATGCATACACACTGAGGGTGAATGAGAAGAGTGACATCTACAGTTTTGGTGTGGTGATTCTGGAATTGGTTACCGGGAAACTTCCAGTTGACCCAGAATTCGGAGAAAAGGACTTGGTGAAGTGGGTCTGTACCACCTTGGATCAGAAAGGAGTGGACCATGTCATTGACCCCAAACTCGATTCTTGTTACAAGGAAGAAGTATGCAAGGTCCTCAATATCGGGCTCCTCTGCACCAGTCCTCTCCCAATCAATAGACCATCGATGAGAAGAGTGGTGAAGTTGTTGCAAGAAGCGGGAACGGAGAAGCATCCTCAGATTAAGAAGGAAGGCAAATTGTCACCATATTACTATGAAGATGCATCGGATCATGGAAGTGTAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

866

Amino Acids

95.7

Weight (kDa)

5.19

Isoelectric Point (pI)

38.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 24 - 63 2e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 88 - 172 4.6e-08 Leucine-rich repeat region
LRR_8 PF13855 91 - 151 1.6e-09 Leucine rich repeat
LRR_4 PF12799 115 - 155 1.8e-08 Leucine Rich repeats (2 copies)
LRR_14 PF23598 182 - 295 2.4e-08 Leucine-rich repeat region
LRR_14 PF23598 490 - 555 3.3e-08 Leucine-rich repeat region
LRR_8 PF13855 499 - 559 1.2e-09 Leucine rich repeat
Pkinase PF00069 723 - 782 6.8e-06 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 725 - 832 3.8e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012987)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28440
fragaria_vesca FvH4_3g08530 FvH4_3g08530
malus_domestica MD05G1309900.v1.1 MD10G1288500.v1.1
prunus_persica Prupe.4G053100_v2.0.a1
pyrus_communis pycom05g28630
rosa_chinensis RchiOBHm_Chr5g0009311
rosa_laevigata RLG00000031647
rosa_multiflora Rmu_sc0006233.1_g000007
rosa_roxburghii Rroxscaffold_1G00066780 Rroxscaffold_1G00067480
rosa_rugosa Rorug04G0441700
rosa_samantha Rh5AG072200 Rh5BG068700 Rh5CG081100 Rh5DG068900
rosa_wichuraiana Rw5G006750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 2002, 2080
AbsI CCTCGAGG 1 cut(s) 641
AccBSI CCGCTC 1 cut(s) 1248
AccI GTMKAC 3 cut(s) 216, 513, 700
AccII CGCG 2 cut(s) 872, 1045
AclWI GGATC 5 cut(s) 604, 617, 1721, 2364, 2589
AcoI YGGCCR 2 cut(s) 1054, 1177
AcsI RAATTY 2 cut(s) 462, 2312
AcuI CTGAAG 1 cut(s) 869
AcvI CACGTG 2 cut(s) 339, 1220
AcyI GRCGYC 2 cut(s) 153, 761
AfaI GTAC 5 cut(s) 1001, 1784, 1949, 2096, 2347
AflIII ACRYGT 6 cut(s) 338, 870, 1217, 1219, 1635, 1779
AgeI ACCGGT 1 cut(s) 1239
AgsI TTSAA 7 cut(s) 232, 1391, 1424, 1591, 1726, 1945, 1960
AjiI CACGTC 1 cut(s) 202
AjnI CCWGG 2 cut(s) 137, 1863
Alw21I GWGCWC 5 cut(s) 624, 801, 1008, 1584, 1758
Alw26I GTCTC 2 cut(s) 1199, 1472
AlwI GGATC 5 cut(s) 604, 617, 1721, 2364, 2589
Ama87I CYCGRG 7 cut(s) 454, 467, 494, 601, 617, 641, 1196
ApeKI GCWGC 4 cut(s) 862, 1039, 1276, 1904
ApoI RAATTY 2 cut(s) 462, 2312
ArsI GACNNNNNNTTYG 2 cut(s) 2297, 2329
AsiGI ACCGGT 1 cut(s) 1239
Asp700I GAANNNNTTC 3 cut(s) 953, 1428, 1457
AspLEI GCGC 1 cut(s) 533
AvaI CYCGRG 7 cut(s) 454, 467, 494, 601, 617, 641, 1196
AvaII GGWCC 6 cut(s) 499, 646, 724, 1513, 2371, 2425
BamHI GGATCC 1 cut(s) 609
BanII GRGCYC 6 cut(s) 624, 801, 1008, 1584, 1758, 2442
BarI GAAGNNNNNNTAC 4 cut(s) 1383, 1415, 1940, 1972
BbrPI CACGTG 2 cut(s) 339, 1220
BbsI GAAGAC 2 cut(s) 11, 1632
Bbv12I GWGCWC 5 cut(s) 624, 801, 1008, 1584, 1758
BbvCI CCTCAGC 2 cut(s) 1070, 1883
BbvI GCAGC 4 cut(s) 849, 1026, 1263, 1891
BccI CCATC 3 cut(s) 314, 1616, 2482
BceAI ACGGC 2 cut(s) 190, 763
BciT130I CCWGG 2 cut(s) 139, 1865
BclI TGATCA 1 cut(s) 1158
BcoDI GTCTC 2 cut(s) 1199, 1472
BfaI CTAG 1 cut(s) 1263
BfmI CTRYAG 3 cut(s) 1433, 1977, 2254
BfoI RGCGCY 1 cut(s) 534
BglI GCCNNNNNGGC 1 cut(s) 1349
BlpI GCTNAGC 1 cut(s) 2108
Bme18I GGWCC 6 cut(s) 499, 646, 724, 1513, 2371, 2425
BmeT110I CYCGRG 7 cut(s) 454, 467, 494, 601, 617, 641, 1196
BmgBI CACGTC 1 cut(s) 202
BmiI GGNNCC 4 cut(s) 388, 611, 894, 2441
BmrI ACTGGG 1 cut(s) 2085
BmsI GCATC 5 cut(s) 186, 1140, 2533, 2563, 2585
BmuI ACTGGG 1 cut(s) 2085
BoxI GACNNNNGTC 1 cut(s) 1220
BpiI GAAGAC 2 cut(s) 11, 1632
BplI GAGNNNNNCTC 2 cut(s) 488, 520
BpmI CTGGAG 3 cut(s) 941, 1316, 2193
Bpu10I CCTNAGC 2 cut(s) 1070, 1883
Bpu1102I GCTNAGC 1 cut(s) 2108
Bsa29I ATCGAT 1 cut(s) 2477
BsaAI YACGTR 2 cut(s) 339, 1220
BsaHI GRCGYC 2 cut(s) 153, 761
BsaJI CCNNGG 8 cut(s) 601, 1043, 1773, 1851, 1863, 1864, 1908, 2352
BsaWI WCCGGW 5 cut(s) 818, 1239, 1402, 1535, 1690
BsaXI ACNNNNNCTCC 8 cut(s) 95, 107, 125, 137, 287, 317, 1820, 1850
Bse118I RCCGGY 7 cut(s) 383, 440, 882, 970, 1031, 1239, 1343
Bse1I ACTGG 7 cut(s) 1096, 1312, 1384, 2046, 2080, 2300, 2451
Bse3DI GCAATG 3 cut(s) 978, 2119, 2202
BseBI CCWGG 2 cut(s) 139, 1865
BseCI ATCGAT 1 cut(s) 2477
BseDI CCNNGG 8 cut(s) 601, 1043, 1773, 1851, 1863, 1864, 1908, 2352
BseGI GGATG 1 cut(s) 2524
BseMI GCAATG 3 cut(s) 978, 2119, 2202
BseMII CTCAG 6 cut(s) 718, 1061, 1874, 2099, 2219, 2543
BseNI ACTGG 7 cut(s) 1096, 1312, 1384, 2046, 2080, 2300, 2451
BseXI GCAGC 4 cut(s) 849, 1026, 1263, 1891
BseYI CCCAGC 4 cut(s) 272, 694, 1228, 2017
BsgI GTGCAG 2 cut(s) 728, 2431
Bsh1236I CGCG 2 cut(s) 872, 1045
BshTI ACCGGT 1 cut(s) 1239
BshVI ATCGAT 1 cut(s) 2477
BsiHKAI GWGCWC 5 cut(s) 624, 801, 1008, 1584, 1758
BsiHKCI CYCGRG 7 cut(s) 454, 467, 494, 601, 617, 641, 1196
BslFI GGGAC 3 cut(s) 185, 547, 2086
BsmAI GTCTC 2 cut(s) 1199, 1472
BsmFI GGGAC 3 cut(s) 185, 547, 2086
BsmI GAATGC 1 cut(s) 1110
BsoBI CYCGRG 7 cut(s) 454, 467, 494, 601, 617, 641, 1196
Bsp1286I GDGCHC 6 cut(s) 624, 801, 1008, 1584, 1758, 2442
Bsp1407I TGTACA 2 cut(s) 1782, 2094
Bsp143I GATC 5 cut(s) 609, 1158, 1713, 2356, 2581
Bsp1720I GCTNAGC 1 cut(s) 2108
BspCNI CTCAG 6 cut(s) 717, 1062, 1875, 2100, 2220, 2542
BspDI ATCGAT 1 cut(s) 2477
BspFNI CGCG 2 cut(s) 872, 1045
BspLI GGNNCC 4 cut(s) 388, 611, 894, 2441
BspMAI CTGCAG 1 cut(s) 1437
BspPI GGATC 5 cut(s) 604, 617, 1721, 2364, 2589
BsrBI CCGCTC 1 cut(s) 1248
BsrDI GCAATG 3 cut(s) 978, 2119, 2202
BsrFI RCCGGY 7 cut(s) 383, 440, 882, 970, 1031, 1239, 1343
BsrGI TGTACA 2 cut(s) 1782, 2094
BsrI ACTGG 7 cut(s) 1096, 1312, 1384, 2046, 2080, 2300, 2451
BssAI RCCGGY 7 cut(s) 383, 440, 882, 970, 1031, 1239, 1343
BssECI CCNNGG 8 cut(s) 601, 1043, 1773, 1851, 1863, 1864, 1908, 2352
BssMI GATC 5 cut(s) 609, 1158, 1713, 2356, 2581
BssNI GRCGYC 2 cut(s) 153, 761
BssT1I CCWWGG 3 cut(s) 1773, 1908, 2352
Bst2UI CCWGG 2 cut(s) 139, 1865
Bst4CI ACNGT 2 cut(s) 1605, 2258
Bst6I CTCTTC 6 cut(s) 26, 50, 242, 263, 2237, 2479
BstACI GRCGYC 2 cut(s) 153, 761
BstAPI GCANNNNNTGC 1 cut(s) 183
BstAUI TGTACA 2 cut(s) 1782, 2094
BstBAI YACGTR 2 cut(s) 339, 1220
BstC8I GCNNGC 6 cut(s) 827, 972, 976, 1037, 1508, 1969
BstDEI CTNAG 8 cut(s) 670, 704, 1070, 1502, 1883, 2108, 2228, 2529
BstDSI CCRYGG 1 cut(s) 1043
BstEII GGTNACC 1 cut(s) 2284
BstF5I GGATG 1 cut(s) 2524
BstFNI CGCG 2 cut(s) 872, 1045
BstH2I RGCGCY 1 cut(s) 534
BstHHI GCGC 1 cut(s) 533
BstKTI GATC 5 cut(s) 612, 1161, 1716, 2359, 2584
BstMAI GTCTC 2 cut(s) 1199, 1472
BstMBI GATC 5 cut(s) 609, 1158, 1713, 2356, 2581
BstMWI GCNNNNNNNGC 8 cut(s) 183, 273, 737, 892, 980, 1349, 1579, 1973
BstNI CCWGG 2 cut(s) 139, 1865
BstNSI RCATGY 4 cut(s) 186, 1286, 1639, 1783
BstPAI GACNNNNGTC 1 cut(s) 1220
BstPI GGTNACC 1 cut(s) 2284
BstSFI CTRYAG 3 cut(s) 1433, 1977, 2254
BstUI CGCG 2 cut(s) 872, 1045
BstV1I GCAGC 4 cut(s) 849, 1026, 1263, 1891
BstV2I GAAGAC 2 cut(s) 11, 1632
BstX2I RGATCY 1 cut(s) 609
BstYI RGATCY 1 cut(s) 609
Bsu15I ATCGAT 1 cut(s) 2477
BsuTUI ATCGAT 1 cut(s) 2477
BtgI CCRYGG 1 cut(s) 1043
BtgZI GCGATG 1 cut(s) 2187
BtrI CACGTC 1 cut(s) 202
BtsCI GGATG 1 cut(s) 2524
BtsI GCAGTG 1 cut(s) 2136
BtsIMutI CAGTG 3 cut(s) 1468, 2136, 2225
Cac8I GCNNGC 6 cut(s) 827, 972, 976, 1037, 1508, 1969
CfoI GCGC 1 cut(s) 533
Cfr10I RCCGGY 7 cut(s) 383, 440, 882, 970, 1031, 1239, 1343
Cfr42I CCGCGG 1 cut(s) 1046
Cfr9I CCCGGG 1 cut(s) 601
ClaI ATCGAT 1 cut(s) 2477
CseI GACGC 4 cut(s) 161, 750, 861, 878
Csp6I GTAC 5 cut(s) 1000, 1783, 1948, 2095, 2346
CspAI ACCGGT 1 cut(s) 1239
CviAII CATG 7 cut(s) 183, 877, 1283, 1636, 1780, 2375, 2585
CviQI GTAC 5 cut(s) 1000, 1783, 1948, 2095, 2346
DdeI CTNAG 8 cut(s) 670, 704, 1070, 1502, 1883, 2108, 2228, 2529
DpnI GATC 5 cut(s) 611, 1160, 1715, 2358, 2583
DpnII GATC 5 cut(s) 609, 1158, 1713, 2356, 2581
DrdI GACNNNNNNGTC 2 cut(s) 2002, 2080
DseDI GACNNNNNNGTC 2 cut(s) 2002, 2080
EaeI YGGCCR 2 cut(s) 1054, 1177
Eam1104I CTCTTC 6 cut(s) 26, 50, 242, 263, 2237, 2479
EarI CTCTTC 6 cut(s) 26, 50, 242, 263, 2237, 2479
Ecl136II GAGCTC 5 cut(s) 622, 799, 1006, 1582, 1756
Eco130I CCWWGG 3 cut(s) 1773, 1908, 2352
Eco147I AGGCCT 2 cut(s) 81, 825
Eco24I GRGCYC 6 cut(s) 624, 801, 1008, 1584, 1758, 2442
Eco47I GGWCC 6 cut(s) 499, 646, 724, 1513, 2371, 2425
Eco53kI GAGCTC 5 cut(s) 622, 799, 1006, 1582, 1756
Eco57I CTGAAG 1 cut(s) 869
Eco72I CACGTG 2 cut(s) 339, 1220
Eco88I CYCGRG 7 cut(s) 454, 467, 494, 601, 617, 641, 1196
Eco91I GGTNACC 1 cut(s) 2284
EcoICRI GAGCTC 5 cut(s) 622, 799, 1006, 1582, 1756
EcoO109I RGGNCCY 3 cut(s) 499, 646, 2425
EcoO65I GGTNACC 1 cut(s) 2284
EcoRI GAATTC 1 cut(s) 2312
EcoRII CCWGG 2 cut(s) 137, 1863
EcoT14I CCWWGG 3 cut(s) 1773, 1908, 2352
EcoT22I ATGCAT 2 cut(s) 2221, 2578
EcoT38I GRGCYC 6 cut(s) 624, 801, 1008, 1584, 1758, 2442
ErhI CCWWGG 3 cut(s) 1773, 1908, 2352
FaeI CATG 7 cut(s) 186, 880, 1286, 1639, 1783, 2378, 2588
FalI AAGNNNNNCTT 1 cut(s) 2581
FaqI GGGAC 3 cut(s) 185, 547, 2086
FatI CATG 7 cut(s) 182, 876, 1282, 1635, 1779, 2374, 2584
FauI CCCGC 3 cut(s) 621, 1241, 2503
FauNDI CATATG 1 cut(s) 1874
FbaI TGATCA 1 cut(s) 1158
FblI GTMKAC 3 cut(s) 216, 513, 700
FokI GGATG 1 cut(s) 2511
FriOI GRGCYC 6 cut(s) 624, 801, 1008, 1584, 1758, 2442
FspBI CTAG 1 cut(s) 1263
GlaI GCGC 1 cut(s) 532
GsaI CCCAGC 4 cut(s) 276, 698, 1232, 2021
GsuI CTGGAG 3 cut(s) 941, 1316, 2193
HaeII RGCGCY 1 cut(s) 534
HgaI GACGC 4 cut(s) 161, 750, 861, 878
HhaI GCGC 1 cut(s) 533
Hin1I GRCGYC 2 cut(s) 153, 761
Hin1II CATG 7 cut(s) 186, 880, 1286, 1639, 1783, 2378, 2588
Hin6I GCGC 1 cut(s) 531
HinP1I GCGC 1 cut(s) 531
HincII GTYRAC 5 cut(s) 217, 701, 768, 777, 2305
HindII GTYRAC 5 cut(s) 217, 701, 768, 777, 2305
Hpy188III TCNNGA 7 cut(s) 456, 467, 478, 902, 1960, 2210, 2276
Hpy99I CGWCG 4 cut(s) 218, 766, 872, 908
HpyAV CCTTC 8 cut(s) 71, 201, 292, 1385, 1526, 1957, 2533, 2537
HpyCH4III ACNGT 2 cut(s) 1605, 2258
HpyCH4IV ACGT 3 cut(s) 201, 338, 1219
HpyF10VI GCNNNNNNNGC 8 cut(s) 183, 273, 737, 892, 980, 1349, 1579, 1973
HpyF3I CTNAG 8 cut(s) 670, 704, 1070, 1502, 1883, 2108, 2228, 2529
HpySE526I ACGT 3 cut(s) 201, 338, 1219
Hsp92I GRCGYC 2 cut(s) 153, 761
Hsp92II CATG 7 cut(s) 186, 880, 1286, 1639, 1783, 2378, 2588
HspAI GCGC 1 cut(s) 531
KroI GCCGGC 1 cut(s) 970
KroNI GCCGGC 1 cut(s) 972
Ksp22I TGATCA 1 cut(s) 1158
KspI CCGCGG 1 cut(s) 1046
Kzo9I GATC 5 cut(s) 609, 1158, 1713, 2356, 2581
LmnI GCTCC 8 cut(s) 392, 922, 1011, 1107, 1297, 1761, 2212, 2445
Lsp1109I GCAGC 4 cut(s) 849, 1026, 1263, 1891
LweI GCATC 5 cut(s) 186, 1140, 2533, 2563, 2585
MaeI CTAG 1 cut(s) 1263
MaeII ACGT 3 cut(s) 201, 338, 1219
MaeIII GTNAC 8 cut(s) 665, 1232, 1491, 2198, 2246, 2284, 2402, 2553
MalI GATC 5 cut(s) 611, 1160, 1715, 2358, 2583
MbiI CCGCTC 1 cut(s) 1248
MboI GATC 5 cut(s) 609, 1158, 1713, 2356, 2581
MflI RGATCY 1 cut(s) 609
MhlI GDGCHC 6 cut(s) 624, 801, 1008, 1584, 1758, 2442
MluI ACGCGT 1 cut(s) 870
MlyI GAGTC 9 cut(s) 116, 479, 683, 696, 907, 980, 1129, 1201, 1564
MmeI TCCRAC 6 cut(s) 115, 138, 351, 376, 587, 1713
Mph1103I ATGCAT 2 cut(s) 2221, 2578
MroNI GCCGGC 1 cut(s) 970
MroXI GAANNNNTTC 3 cut(s) 953, 1428, 1457
MseI TTAA 5 cut(s) 632, 735, 1488, 2535, 2599
MslI CAYNNNNRTG 2 cut(s) 1524, 2589
MspA1I CMGCKG 3 cut(s) 1045, 1069, 1971
Mva1269I GAATGC 1 cut(s) 1110
MvaI CCWGG 2 cut(s) 139, 1865
MvnI CGCG 2 cut(s) 872, 1045
MwoI GCNNNNNNNGC 8 cut(s) 183, 273, 737, 892, 980, 1349, 1579, 1973
NaeI GCCGGC 1 cut(s) 972
NdeI CATATG 1 cut(s) 1874
NdeII GATC 5 cut(s) 609, 1158, 1713, 2356, 2581
NgoMIV GCCGGC 1 cut(s) 970
NlaIII CATG 7 cut(s) 186, 880, 1286, 1639, 1783, 2378, 2588
NlaIV GGNNCC 4 cut(s) 388, 611, 894, 2441
NmeAIII GCCGAG 4 cut(s) 528, 895, 1032, 1876
NmuCI GTSAC 3 cut(s) 1232, 2246, 2553
NsiI ATGCAT 2 cut(s) 2221, 2578
NspI RCATGY 4 cut(s) 186, 1286, 1639, 1783
PaeR7I CTCGAG 2 cut(s) 494, 641
PasI CCCWGGG 1 cut(s) 1864
PceI AGGCCT 2 cut(s) 81, 825
PciI ACATGT 2 cut(s) 1635, 1779
PctI GAATGC 1 cut(s) 1110
PdiI GCCGGC 1 cut(s) 972
PdmI GAANNNNTTC 3 cut(s) 953, 1428, 1457
PflFI GACNNNGTC 1 cut(s) 2375
PfoI TCCNGGA 4 cut(s) 137, 1103, 1607, 1664
PinAI ACCGGT 1 cut(s) 1239
PleI GAGTC 9 cut(s) 116, 478, 683, 696, 907, 980, 1128, 1200, 1563
PmaCI CACGTG 2 cut(s) 339, 1220
PmlI CACGTG 2 cut(s) 339, 1220
PpsI GAGTC 9 cut(s) 116, 478, 683, 696, 907, 980, 1128, 1200, 1563
Ppu21I YACGTR 2 cut(s) 339, 1220
PpuMI RGGWCCY 3 cut(s) 499, 646, 2425
PscI ACATGT 2 cut(s) 1635, 1779
PshAI GACNNNNGTC 1 cut(s) 1220
Psp124BI GAGCTC 5 cut(s) 624, 801, 1008, 1584, 1758
Psp5II RGGWCCY 3 cut(s) 499, 646, 2425
Psp6I CCWGG 2 cut(s) 137, 1863
PspCI CACGTG 2 cut(s) 339, 1220
PspEI GGTNACC 1 cut(s) 2284
PspFI CCCAGC 4 cut(s) 272, 694, 1228, 2017
PspGI CCWGG 2 cut(s) 137, 1863
PspN4I GGNNCC 4 cut(s) 388, 611, 894, 2441
PspPPI RGGWCCY 3 cut(s) 499, 646, 2425
PspXI VCTCGAGB 2 cut(s) 494, 641
PstI CTGCAG 1 cut(s) 1437
PsuI RGATCY 1 cut(s) 609
PsyI GACNNNGTC 1 cut(s) 2375
RsaI GTAC 5 cut(s) 1001, 1784, 1949, 2096, 2347
RsaNI GTAC 5 cut(s) 1000, 1783, 1948, 2095, 2346
RseI CAYNNNNRTG 2 cut(s) 1524, 2589
SacI GAGCTC 5 cut(s) 624, 801, 1008, 1584, 1758
SacII CCGCGG 1 cut(s) 1046
SalI GTCGAC 2 cut(s) 215, 699
SaqAI TTAA 5 cut(s) 632, 735, 1488, 2535, 2599
Sau3AI GATC 5 cut(s) 609, 1158, 1713, 2356, 2581
SchI GAGTC 9 cut(s) 116, 479, 683, 696, 907, 980, 1129, 1201, 1564
SduI GDGCHC 6 cut(s) 624, 801, 1008, 1584, 1758, 2442
SfaNI GCATC 5 cut(s) 186, 1140, 2533, 2563, 2585
SfcI CTRYAG 3 cut(s) 1433, 1977, 2254
Sfr274I CTCGAG 2 cut(s) 494, 641
Sfr303I CCGCGG 1 cut(s) 1046
SgrAI CRCCGGYG 1 cut(s) 1031
SgrBI CCGCGG 1 cut(s) 1046
SinI GGWCC 6 cut(s) 499, 646, 724, 1513, 2371, 2425
SlaI CTCGAG 2 cut(s) 494, 641
SmaI CCCGGG 1 cut(s) 603
SmiMI CAYNNNNRTG 2 cut(s) 1524, 2589
SmlI CTYRAG 2 cut(s) 494, 641
SmoI CTYRAG 2 cut(s) 494, 641
SseBI AGGCCT 2 cut(s) 81, 825
SspMI CTAG 1 cut(s) 1263
SstI GAGCTC 5 cut(s) 624, 801, 1008, 1584, 1758
StuI AGGCCT 2 cut(s) 81, 825
StyI CCWWGG 3 cut(s) 1773, 1908, 2352
TaaI ACNGT 2 cut(s) 1605, 2258
TaiI ACGT 3 cut(s) 204, 341, 1222
TaqII GACCGA 1 cut(s) 674
TatI WGTACW 3 cut(s) 1782, 1947, 2094
TauI GCSGC 7 cut(s) 267, 832, 1045, 1069, 1282, 1352, 1449
Tru1I TTAA 5 cut(s) 632, 735, 1488, 2535, 2599
Tru9I TTAA 5 cut(s) 632, 735, 1488, 2535, 2599
TscAI CASTG 3 cut(s) 1468, 2136, 2232
TseFI GTSAC 3 cut(s) 1232, 2246, 2553
TseI GCWGC 4 cut(s) 862, 1039, 1276, 1904
Tsp45I GTSAC 3 cut(s) 1232, 2246, 2553
TspDTI ATGAA 5 cut(s) 17, 893, 1885, 2070, 2583
TspGWI ACGGA 2 cut(s) 202, 2531
TspMI CCCGGG 1 cut(s) 601
TspRI CASTG 3 cut(s) 1468, 2136, 2232
Tth111I GACNNNGTC 1 cut(s) 2375
VpaK11BI GGWCC 6 cut(s) 499, 646, 724, 1513, 2371, 2425
XapI RAATTY 2 cut(s) 462, 2312
XceI RCATGY 4 cut(s) 186, 1286, 1639, 1783
XhoI CTCGAG 2 cut(s) 494, 641
XmaI CCCGGG 1 cut(s) 601
XmiI GTMKAC 3 cut(s) 216, 513, 700
XmnI GAANNNNTTC 3 cut(s) 953, 1428, 1457
XspI CTAG 1 cut(s) 1263
Zsp2I ATGCAT 2 cut(s) 2221, 2578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.