RLG00000031647
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0012987
Physical Location & Seq
Forward (+)
7025673 .. 7028904
3232 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031647

Sequence Viewer

Length: 2994 bp
ATGAAGACAAAAGTGCTTCTCTTCTTCCTCCTCCCTCTCCTCCTCTTCTCCTCCACCACCTCCTCCTCCCTCACCCAAGAAGGCCTCTACCTCCAACACATCAAACACTCCCTCTCCGACCCCGACTCCTCCCTCTCCTCCTGGAACGACCAAGACGCCACCCCTTGCTCATGGGACGGCATCACATGCAACCCTTCCACCCACGTGATCACCTCCGTCGACCTCTCCAGCTTCAACCTCTTTGGCCCTTTCCCCTCCCTCCTCTGCCGCCTCCCTAACCTCTCCTTCCTCTCCCTCTACAACAACTCCATCAACTCCACCCTCCCCTCCGACATCTCCACGTGTCAGAACCTCCAACACCTCGACCTCTCCCAGAACCTCCTCACCGGCTCCCTCCCCTCCGCCCTTGCCGACCTCCCCAACCTCCGCTACCTCGACCTCACCGGCAACAACTTCTCGGGAGAAATTCCCGAGTCTTTCGGGAGATTCCAAAAACTCGAGGTCCTCTCTCTCGTCTACAACCTCCTCGACAGCGCCCTGCCCCCGTTTCTCGGCAATATCTCGTCCCTCAAAATGCTCAACCTTTCTTACAACCCGTTTTTCCCGGGTCGGATCCCGCCCGAGCTCGGAAACTTAACGAACCTCGAGGTCCTCTGGCTGACCGAGTGTAACTTAGTCGGCGAAATACCCGACTCGCTGGGTCGACTCAGTAAACTCACCGATTTGGACCTCGCCATTAACGCCCTGCACGGCCCGATTCCGGCGTCGCTGACCGAGTTGACCAGCGTGGTTCAGATTGAGCTCTATAACAACACCTTGACCGGAGGCCTGCCGCCGGGAATGTCGAAGCTGAAGAAGCTCCGGCTGCTCGACGCGTCCATGAACCGGCTGACGGGGCCGATTCCCGACGAGTTGACTCGGCTGGAGCTGGAAAGCCTGAATCTTTACGAGAACAACTTCGAGGGGAGTTTGCCAGCGAGCATTGCCGACTCGCCGAACCTGTACGAGCTCCGGCTGTTTCAGAACAAGCTCACCGGCGAGCTGCCGCGGAATCTCGGCCAGAACTCGCCGCTGAGGTGGATAGACCTGTCGAACAACCAGTTTTCCGGGAGTATTCCGGCGAGTCTGTGCGAAAAGGGGCAAATTGAGGAGATGCTGATGATCAACAATGCTTTTTCCGGCGAGATTCCGGCGAGTCTCGGGGAGTGTCAGAGCTTGACACGTGTCCGGTTGGGTCACAACCGGTTGAGCGGTGAAGTTCCGGCTAGTTTCTGGGGGCTGCCGCATGTCTATTTGCTCGAGCTGGTTGATAATCAGTTATCCGGGCCGATAGCCAAGACTATTGCCGGTGCGGCTAACCTTTCGCTTCTGATAATCGAAAGGAACAAGTTCGAAGGTTCGATACCGGAGGAAATTGGGTGGGTTGAAAATCTTCTGCAGTTTTCCGGCAGTGACAATAGGTTCAGTGGGCCTTTGCCGGAGACAATAGTTAGGTTACGACATCTTAGCACGCTGGACCTTCACAACAATGAGCTTTCCGGTGAGTTTCCGAGTGGGAGTCAGTCTTTGGTTCGGCTAAATGAGCTGAATTTGAAGAATAACCAACTGTCCGGGAAAATCCCGGATGGAATTGGGAACATGTCTTCGCTGAATTACCTTGATCTCTCCGGGAATCGGTTTTCGGGTCAAGTTCCGGTTTGGTTGCAGAATATGAAGATCAATGTGTTCAATTTGTCGGACAACCAGCTTTCGGGTGAGCTCCCTCCTTTGTTTGCCAAGGAGATGTACAGAAATAGCTTTCTGGGAAATCCGGGTCTTTGTGGAGATTTGGAGGGGTTGTGTGAGAGCAGAGCCGAGGAGAAAACCCTGGGCTACATATGGTTGCTGAGGTGCATTTTCATTCTGGCTGCCTTGGTGTTTGTGGTGGGTGTGGTTTGGTTTTACTTCAAGTACAAGAACTTCAAGAAGGCCAGCGGTGCTATAGATAAATCGAAGTGGACTTTGATGTCGTTTCACAAGCTGGGTTTCAGCGAGTATGAGATATTGGATTGCCTTGATGAAGATAATGTGATTGGGACTGGGGGGTCTGGGAAAGTGTACAAGGTTGTGCTGAGCAATCGTGAGGTTGTTGCAGTGAAAAAGCTGTGGAGAGGGAAAGTGAAGGAGTGTGAAAGTGATGATGTTGAGAAGGGTTGGGTCCAAGATGATGGATTTGAGGCAGAGGTCGACACTTTGGGGAGGATTAGGCACAAGAACATTGTGAAGCTTTGGTGCTGTTGTACCGCTAGAGATTGCAAGCTTTTGGTGTACGAGTACATGCCAAATGGTAGTCTTGGTGATTTGCTGCATAGTAGCAAAGCAGGGTTGCTGGATTGGCCTACAAGGTTCAAGATTGCTCTTGATGCTGCTGATGGGCTTTCGTATCTACATCATGATTGTGTTCCTGCTATTGTTCATAGGGATGTGAAATCAAACAATATATTGTTGGATGGGGATTTCGGAGCCAGAGTAGCGGATTTTGGAGTAGCTAAGGTCGTTGATGCAACTGGAAAAGGGGCTAAATCCATGTCGGTCATTGCAGGTTCTTGTGGTTACATTGCTCCAGAATATGCATACACACTGAGGGTGAATGAGAAGAGTGACATCTACAGTTTTGGTGTGGTGATTCTGGAATTGGTTACTGGGAAACTTCCTGTTGACCCAGAATTCGGAGAAAAGGACTTGGTGAAGTGGGTCTGTACCACCTTGGATCAGAAAGGAGTGGACCATGTCATTGACCCCAAACTCGATTCTTGTTACAAGGAAGAAGTATGCAAGGTCCTCAATATCGGGCTCCTCTGCACCAGTCCTCTCCCAATCAATAGACCATCGATGAGAAGAGTGGTGAAGTTGTTGCAAGAAGCAGGAACGGAGAAGCATCCTCAGATTAAGAAGGAAGGCAAATTGTCACCATATTACTATGAAGATGCATCGGATCATGGAAGTGTAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

998

Amino Acids

110.11

Weight (kDa)

5.31

Isoelectric Point (pI)

36.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 25 - 64 1.1e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 88 - 193 6.9e-09 Leucine-rich repeat region
LRR_8 PF13855 92 - 152 1.8e-09 Leucine rich repeat
LRR_4 PF12799 116 - 156 5.7e-09 Leucine Rich repeats (2 copies)
LRR_14 PF23598 184 - 337 1.3e-08 Leucine-rich repeat region
LRR_14 PF23598 491 - 556 2.4e-08 Leucine-rich repeat region
LRR_8 PF13855 500 - 560 2.4e-08 Leucine rich repeat
Pkinase PF00069 688 - 913 3e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 688 - 963 1.4e-35 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012987)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28440
fragaria_vesca FvH4_3g08530 FvH4_3g08530
malus_domestica MD05G1309900.v1.1 MD10G1288500.v1.1
prunus_persica Prupe.4G053100_v2.0.a1
pyrus_communis pycom05g28630
rosa_chinensis RchiOBHm_Chr5g0009311
rosa_laevigata RLG00000031647
rosa_multiflora Rmu_sc0006233.1_g000007
rosa_roxburghii Rroxscaffold_1G00066780 Rroxscaffold_1G00067480
rosa_rugosa Rorug04G0441700
rosa_samantha Rh5AG072200 Rh5BG068700 Rh5CG081100 Rh5DG068900
rosa_wichuraiana Rw5G006750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 2005, 2083
AbsI CCTCGAGG 1 cut(s) 644
Acc36I ACCTGC 1 cut(s) 2570
AccBSI CCGCTC 1 cut(s) 1251
AccI GTMKAC 4 cut(s) 219, 516, 703, 2226
AccII CGCG 2 cut(s) 875, 1048
AclWI GGATC 4 cut(s) 607, 620, 2757, 2982
AcoI YGGCCR 1 cut(s) 1057
AcsI RAATTY 3 cut(s) 465, 1588, 2705
AcuI CTGAAG 1 cut(s) 872
AcvI CACGTG 3 cut(s) 205, 342, 1223
AcyI GRCGYC 2 cut(s) 156, 764
AfaI GTAC 8 cut(s) 1004, 1787, 1952, 2099, 2281, 2309, 2315, 2740
AfiI CCNNNNNNNGG 9 cut(s) 551, 626, 760, 835, 885, 892, 1674, 1750, 2708
AflIII ACRYGT 5 cut(s) 341, 873, 1220, 1222, 1638
AgeI ACCGGT 1 cut(s) 1242
AgsI TTSAA 7 cut(s) 235, 1427, 1594, 1729, 1948, 1963, 2389
AjnI CCWGG 2 cut(s) 140, 1866
AleI CACNNNNGTG 1 cut(s) 203
Alw21I GWGCWC 4 cut(s) 627, 804, 1011, 1761
Alw26I GTCTC 2 cut(s) 1202, 1475
AlwI GGATC 4 cut(s) 607, 620, 2757, 2982
Ama87I CYCGRG 8 cut(s) 457, 470, 497, 604, 620, 644, 1199, 1298
ApeKI GCWGC 6 cut(s) 865, 1042, 1279, 1907, 2344, 2405
ApoI RAATTY 3 cut(s) 465, 1588, 2705
ArsI GACNNNNNNTTYG 2 cut(s) 2690, 2722
AsiGI ACCGGT 1 cut(s) 1242
Asp700I GAANNNNTTC 3 cut(s) 956, 1388, 1431
AspLEI GCGC 1 cut(s) 536
AsuC2I CCSGG 9 cut(s) 605, 606, 837, 1108, 1324, 1612, 1622, 1669, 1812
AsuII TTCGAA 1 cut(s) 1392
AvaI CYCGRG 8 cut(s) 457, 470, 497, 604, 620, 644, 1199, 1298
AvaII GGWCC 7 cut(s) 502, 649, 727, 1516, 2197, 2764, 2818
BamHI GGATCC 1 cut(s) 612
BanII GRGCYC 5 cut(s) 627, 804, 1011, 1761, 2835
BarI GAAGNNNNNNTAC 4 cut(s) 1386, 1418, 1943, 1975
BbrPI CACGTG 3 cut(s) 205, 342, 1223
BbsI GAAGAC 2 cut(s) 11, 1635
Bbv12I GWGCWC 4 cut(s) 627, 804, 1011, 1761
BbvCI CCTCAGC 2 cut(s) 1073, 1886
BbvI GCAGC 6 cut(s) 852, 1029, 1266, 1894, 2331, 2392
BccI CCATC 6 cut(s) 317, 1619, 2201, 2405, 2483, 2875
BceAI ACGGC 2 cut(s) 193, 766
BciT130I CCWGG 2 cut(s) 142, 1868
BclI TGATCA 2 cut(s) 207, 1161
BcnI CCSGG 9 cut(s) 605, 606, 837, 1108, 1324, 1612, 1622, 1669, 1812
BcoDI GTCTC 2 cut(s) 1202, 1475
BfaI CTAG 2 cut(s) 1266, 2286
BfmI CTRYAG 3 cut(s) 1436, 1980, 2647
BfoI RGCGCY 1 cut(s) 537
BfuAI ACCTGC 1 cut(s) 2570
BglI GCCNNNNNGGC 1 cut(s) 1352
BlpI GCTNAGC 1 cut(s) 2111
Bme18I GGWCC 7 cut(s) 502, 649, 727, 1516, 2197, 2764, 2818
BmeT110I CYCGRG 8 cut(s) 457, 470, 497, 604, 620, 644, 1199, 1298
BmiI GGNNCC 6 cut(s) 391, 614, 897, 2198, 2503, 2834
BmrI ACTGGG 2 cut(s) 2088, 2691
BmsI GCATC 7 cut(s) 189, 1143, 2392, 2530, 2926, 2956, 2978
BmuI ACTGGG 2 cut(s) 2088, 2691
BoxI GACNNNNGTC 1 cut(s) 1223
BpiI GAAGAC 2 cut(s) 11, 1635
BplI GAGNNNNNCTC 2 cut(s) 491, 523
BpmI CTGGAG 3 cut(s) 211, 944, 2586
Bpu10I CCTNAGC 3 cut(s) 1073, 1886, 2529
Bpu1102I GCTNAGC 1 cut(s) 2111
Bpu14I TTCGAA 1 cut(s) 1392
BpuMI CCSGG 9 cut(s) 605, 606, 837, 1108, 1324, 1612, 1622, 1669, 1812
Bsa29I ATCGAT 1 cut(s) 2870
BsaAI YACGTR 3 cut(s) 205, 342, 1223
BsaHI GRCGYC 2 cut(s) 156, 764
BsaJI CCNNGG 8 cut(s) 604, 1046, 1776, 1854, 1866, 1867, 1911, 2745
BsaWI WCCGGW 6 cut(s) 821, 1227, 1242, 1405, 1538, 1693
Bsc4I CCNNNNNNNGG 9 cut(s) 551, 626, 760, 835, 885, 892, 1674, 1750, 2708
Bse118I RCCGGY 6 cut(s) 386, 443, 885, 1034, 1242, 1346
Bse1I ACTGG 5 cut(s) 1099, 2083, 2551, 2686, 2844
Bse3DI GCAATG 3 cut(s) 981, 2574, 2595
BseBI CCWGG 2 cut(s) 142, 1868
BseCI ATCGAT 1 cut(s) 2870
BseDI CCNNGG 8 cut(s) 604, 1046, 1776, 1854, 1866, 1867, 1911, 2745
BseGI GGATG 4 cut(s) 1630, 2467, 2494, 2917
BseLI CCNNNNNNNGG 9 cut(s) 551, 626, 760, 835, 885, 892, 1674, 1750, 2708
BseMI GCAATG 3 cut(s) 981, 2574, 2595
BseMII CTCAG 6 cut(s) 721, 1064, 1877, 2102, 2612, 2936
BseNI ACTGG 5 cut(s) 1099, 2083, 2551, 2686, 2844
BseXI GCAGC 6 cut(s) 852, 1029, 1266, 1894, 2331, 2392
BseYI CCCAGC 2 cut(s) 697, 2020
BsgI GTGCAG 2 cut(s) 731, 2824
Bsh1236I CGCG 2 cut(s) 875, 1048
BshTI ACCGGT 1 cut(s) 1242
BshVI ATCGAT 1 cut(s) 2870
BsiHKAI GWGCWC 4 cut(s) 627, 804, 1011, 1761
BsiHKCI CYCGRG 8 cut(s) 457, 470, 497, 604, 620, 644, 1199, 1298
BslFI GGGAC 3 cut(s) 188, 550, 2089
BslI CCNNNNNNNGG 9 cut(s) 551, 626, 760, 835, 885, 892, 1674, 1750, 2708
BsmAI GTCTC 2 cut(s) 1202, 1475
BsmFI GGGAC 3 cut(s) 188, 550, 2089
BsoBI CYCGRG 8 cut(s) 457, 470, 497, 604, 620, 644, 1199, 1298
Bsp119I TTCGAA 1 cut(s) 1392
Bsp1286I GDGCHC 5 cut(s) 627, 804, 1011, 1761, 2835
Bsp1407I TGTACA 2 cut(s) 1785, 2097
Bsp143I GATC 7 cut(s) 207, 612, 1161, 1660, 1716, 2749, 2974
Bsp1720I GCTNAGC 1 cut(s) 2111
BspCNI CTCAG 6 cut(s) 720, 1065, 1878, 2103, 2613, 2935
BspDI ATCGAT 1 cut(s) 2870
BspFNI CGCG 2 cut(s) 875, 1048
BspHI TCATGA 1 cut(s) 2431
BspLI GGNNCC 6 cut(s) 391, 614, 897, 2198, 2503, 2834
BspMAI CTGCAG 1 cut(s) 1440
BspMI ACCTGC 1 cut(s) 2570
BspPI GGATC 4 cut(s) 607, 620, 2757, 2982
BspT104I TTCGAA 1 cut(s) 1392
BsrBI CCGCTC 1 cut(s) 1251
BsrDI GCAATG 3 cut(s) 981, 2574, 2595
BsrFI RCCGGY 6 cut(s) 386, 443, 885, 1034, 1242, 1346
BsrGI TGTACA 2 cut(s) 1785, 2097
BsrI ACTGG 5 cut(s) 1099, 2083, 2551, 2686, 2844
BssAI RCCGGY 6 cut(s) 386, 443, 885, 1034, 1242, 1346
BssECI CCNNGG 8 cut(s) 604, 1046, 1776, 1854, 1866, 1867, 1911, 2745
BssMI GATC 7 cut(s) 207, 612, 1161, 1660, 1716, 2749, 2974
BssNI GRCGYC 2 cut(s) 156, 764
BssT1I CCWWGG 3 cut(s) 1776, 1911, 2745
Bst2UI CCWGG 2 cut(s) 142, 1868
Bst4CI ACNGT 2 cut(s) 1608, 2651
Bst6I CTCTTC 4 cut(s) 26, 50, 2630, 2872
BstACI GRCGYC 2 cut(s) 156, 764
BstAPI GCANNNNNTGC 1 cut(s) 186
BstAUI TGTACA 2 cut(s) 1785, 2097
BstBAI YACGTR 3 cut(s) 205, 342, 1223
BstBI TTCGAA 1 cut(s) 1392
BstC8I GCNNGC 7 cut(s) 830, 975, 979, 1040, 1511, 1972, 2297
BstDEI CTNAG 9 cut(s) 673, 707, 1073, 1505, 1886, 2111, 2529, 2621, 2922
BstDSI CCRYGG 1 cut(s) 1046
BstF5I GGATG 4 cut(s) 1630, 2467, 2494, 2917
BstFNI CGCG 2 cut(s) 875, 1048
BstH2I RGCGCY 1 cut(s) 537
BstHHI GCGC 1 cut(s) 536
BstKTI GATC 7 cut(s) 210, 615, 1164, 1663, 1719, 2752, 2977
BstMAI GTCTC 2 cut(s) 1202, 1475
BstMBI GATC 7 cut(s) 207, 612, 1161, 1660, 1716, 2749, 2974
BstNI CCWGG 2 cut(s) 142, 1868
BstNSI RCATGY 4 cut(s) 189, 1289, 1642, 2320
BstPAI GACNNNNGTC 1 cut(s) 1223
BstSFI CTRYAG 3 cut(s) 1436, 1980, 2647
BstUI CGCG 2 cut(s) 875, 1048
BstV1I GCAGC 6 cut(s) 852, 1029, 1266, 1894, 2331, 2392
BstV2I GAAGAC 2 cut(s) 11, 1635
BstX2I RGATCY 1 cut(s) 612
BstXI CCANNNNNNTGG 1 cut(s) 2207
BstYI RGATCY 1 cut(s) 612
Bsu15I ATCGAT 1 cut(s) 2870
BsuTUI ATCGAT 1 cut(s) 2870
BtgI CCRYGG 1 cut(s) 1046
BtsCI GGATG 4 cut(s) 1630, 2467, 2494, 2917
BtsI GCAGTG 2 cut(s) 1456, 2139
BtsIMutI CAGTG 4 cut(s) 1456, 1471, 2139, 2618
BveI ACCTGC 1 cut(s) 2570
Cac8I GCNNGC 7 cut(s) 830, 975, 979, 1040, 1511, 1972, 2297
CciI TCATGA 1 cut(s) 2431
CfoI GCGC 1 cut(s) 536
Cfr10I RCCGGY 6 cut(s) 386, 443, 885, 1034, 1242, 1346
Cfr42I CCGCGG 1 cut(s) 1049
Cfr9I CCCGGG 1 cut(s) 604
ClaI ATCGAT 1 cut(s) 2870
CseI GACGC 4 cut(s) 164, 753, 864, 881
Csp6I GTAC 8 cut(s) 1003, 1786, 1951, 2098, 2280, 2308, 2314, 2739
CspAI ACCGGT 1 cut(s) 1242
CviQI GTAC 8 cut(s) 1003, 1786, 1951, 2098, 2280, 2308, 2314, 2739
DdeI CTNAG 9 cut(s) 673, 707, 1073, 1505, 1886, 2111, 2529, 2621, 2922
DpnI GATC 7 cut(s) 209, 614, 1163, 1662, 1718, 2751, 2976
DpnII GATC 7 cut(s) 207, 612, 1161, 1660, 1716, 2749, 2974
DrdI GACNNNNNNGTC 2 cut(s) 2005, 2083
DseDI GACNNNNNNGTC 2 cut(s) 2005, 2083
EaeI YGGCCR 1 cut(s) 1057
Eam1104I CTCTTC 4 cut(s) 26, 50, 2630, 2872
EarI CTCTTC 4 cut(s) 26, 50, 2630, 2872
EciI GGCGGA 1 cut(s) 391
Ecl136II GAGCTC 4 cut(s) 625, 802, 1009, 1759
Eco130I CCWWGG 3 cut(s) 1776, 1911, 2745
Eco147I AGGCCT 2 cut(s) 84, 828
Eco24I GRGCYC 5 cut(s) 627, 804, 1011, 1761, 2835
Eco47I GGWCC 7 cut(s) 502, 649, 727, 1516, 2197, 2764, 2818
Eco53kI GAGCTC 4 cut(s) 625, 802, 1009, 1759
Eco57I CTGAAG 1 cut(s) 872
Eco72I CACGTG 3 cut(s) 205, 342, 1223
Eco88I CYCGRG 8 cut(s) 457, 470, 497, 604, 620, 644, 1199, 1298
EcoICRI GAGCTC 4 cut(s) 625, 802, 1009, 1759
EcoO109I RGGNCCY 3 cut(s) 502, 649, 2818
EcoRI GAATTC 1 cut(s) 2705
EcoRII CCWGG 2 cut(s) 140, 1866
EcoT14I CCWWGG 3 cut(s) 1776, 1911, 2745
EcoT22I ATGCAT 2 cut(s) 2614, 2971
EcoT38I GRGCYC 5 cut(s) 627, 804, 1011, 1761, 2835
ErhI CCWWGG 3 cut(s) 1776, 1911, 2745
FaqI GGGAC 3 cut(s) 188, 550, 2089
FauI CCCGC 1 cut(s) 624
FauNDI CATATG 1 cut(s) 1877
FbaI TGATCA 2 cut(s) 207, 1161
FblI GTMKAC 4 cut(s) 219, 516, 703, 2226
FokI GGATG 4 cut(s) 1637, 2474, 2501, 2904
FriOI GRGCYC 5 cut(s) 627, 804, 1011, 1761, 2835
FspBI CTAG 2 cut(s) 1266, 2286
GlaI GCGC 1 cut(s) 535
GsaI CCCAGC 2 cut(s) 701, 2024
GsuI CTGGAG 3 cut(s) 211, 944, 2586
HaeII RGCGCY 1 cut(s) 537
HgaI GACGC 4 cut(s) 164, 753, 864, 881
HhaI GCGC 1 cut(s) 536
Hin1I GRCGYC 2 cut(s) 156, 764
Hin6I GCGC 1 cut(s) 534
HinP1I GCGC 1 cut(s) 534
HincII GTYRAC 6 cut(s) 220, 704, 780, 915, 2227, 2698
HindII GTYRAC 6 cut(s) 220, 704, 780, 915, 2227, 2698
HindIII AAGCTT 2 cut(s) 2264, 2297
Hpy99I CGWCG 4 cut(s) 221, 769, 875, 911
HpyCH4III ACNGT 2 cut(s) 1608, 2651
HpyCH4IV ACGT 3 cut(s) 204, 341, 1222
HpyF3I CTNAG 9 cut(s) 673, 707, 1073, 1505, 1886, 2111, 2529, 2621, 2922
HpySE526I ACGT 3 cut(s) 204, 341, 1222
Hsp92I GRCGYC 2 cut(s) 156, 764
HspAI GCGC 1 cut(s) 534
Ksp22I TGATCA 2 cut(s) 207, 1161
KspI CCGCGG 1 cut(s) 1049
Kzo9I GATC 7 cut(s) 207, 612, 1161, 1660, 1716, 2749, 2974
LmnI GCTCC 8 cut(s) 395, 864, 925, 1014, 1764, 2501, 2605, 2838
Lsp1109I GCAGC 6 cut(s) 852, 1029, 1266, 1894, 2331, 2392
LweI GCATC 7 cut(s) 189, 1143, 2392, 2530, 2926, 2956, 2978
MaeI CTAG 2 cut(s) 1266, 2286
MaeII ACGT 3 cut(s) 204, 341, 1222
MaeIII GTNAC 9 cut(s) 668, 1235, 1451, 1494, 2591, 2639, 2677, 2795, 2946
MalI GATC 7 cut(s) 209, 614, 1163, 1662, 1718, 2751, 2976
MbiI CCGCTC 1 cut(s) 1251
MboI GATC 7 cut(s) 207, 612, 1161, 1660, 1716, 2749, 2974
MflI RGATCY 1 cut(s) 612
MhlI GDGCHC 5 cut(s) 627, 804, 1011, 1761, 2835
MluI ACGCGT 1 cut(s) 873
MlyI GAGTC 9 cut(s) 119, 482, 686, 699, 910, 983, 1132, 1204, 1567
MmeI TCCRAC 7 cut(s) 118, 141, 354, 379, 590, 1716, 2466
Mph1103I ATGCAT 2 cut(s) 2614, 2971
MroXI GAANNNNTTC 3 cut(s) 956, 1388, 1431
MseI TTAA 3 cut(s) 635, 738, 2928
MslI CAYNNNNRTG 5 cut(s) 203, 1527, 2436, 2460, 2982
MspA1I CMGCKG 3 cut(s) 1048, 1072, 1974
MvaI CCWGG 2 cut(s) 142, 1868
MvnI CGCG 2 cut(s) 875, 1048
NciI CCSGG 9 cut(s) 605, 606, 837, 1108, 1324, 1612, 1622, 1669, 1812
NdeI CATATG 1 cut(s) 1877
NdeII GATC 7 cut(s) 207, 612, 1161, 1660, 1716, 2749, 2974
NlaIV GGNNCC 6 cut(s) 391, 614, 897, 2198, 2503, 2834
NmeAIII GCCGAG 4 cut(s) 531, 898, 1035, 1879
NmuCI GTSAC 4 cut(s) 1235, 1451, 2639, 2946
NsiI ATGCAT 2 cut(s) 2614, 2971
NspI RCATGY 4 cut(s) 189, 1289, 1642, 2320
NspV TTCGAA 1 cut(s) 1392
OliI CACNNNNGTG 1 cut(s) 203
PaeR7I CTCGAG 3 cut(s) 497, 644, 1298
PagI TCATGA 1 cut(s) 2431
PasI CCCWGGG 1 cut(s) 1867
PceI AGGCCT 2 cut(s) 84, 828
PciI ACATGT 1 cut(s) 1638
PdmI GAANNNNTTC 3 cut(s) 956, 1388, 1431
PfeI GAWTC 9 cut(s) 486, 757, 901, 940, 1051, 1186, 1672, 2665, 2789
PflFI GACNNNGTC 1 cut(s) 2768
PfoI TCCNGGA 5 cut(s) 140, 1106, 1610, 1620, 1667
PinAI ACCGGT 1 cut(s) 1242
PleI GAGTC 9 cut(s) 119, 481, 686, 699, 910, 983, 1131, 1203, 1566
PmaCI CACGTG 3 cut(s) 205, 342, 1223
PmlI CACGTG 3 cut(s) 205, 342, 1223
PpsI GAGTC 9 cut(s) 119, 481, 686, 699, 910, 983, 1131, 1203, 1566
Ppu21I YACGTR 3 cut(s) 205, 342, 1223
PpuMI RGGWCCY 3 cut(s) 502, 649, 2818
PscI ACATGT 1 cut(s) 1638
PshAI GACNNNNGTC 1 cut(s) 1223
Psp124BI GAGCTC 4 cut(s) 627, 804, 1011, 1761
Psp5II RGGWCCY 3 cut(s) 502, 649, 2818
Psp6I CCWGG 2 cut(s) 140, 1866
PspCI CACGTG 3 cut(s) 205, 342, 1223
PspFI CCCAGC 2 cut(s) 697, 2020
PspGI CCWGG 2 cut(s) 140, 1866
PspN4I GGNNCC 6 cut(s) 391, 614, 897, 2198, 2503, 2834
PspPPI RGGWCCY 3 cut(s) 502, 649, 2818
PspXI VCTCGAGB 3 cut(s) 497, 644, 1298
PstI CTGCAG 1 cut(s) 1440
PsuI RGATCY 1 cut(s) 612
PsyI GACNNNGTC 1 cut(s) 2768
RsaI GTAC 8 cut(s) 1004, 1787, 1952, 2099, 2281, 2309, 2315, 2740
RsaNI GTAC 8 cut(s) 1003, 1786, 1951, 2098, 2280, 2308, 2314, 2739
RseI CAYNNNNRTG 5 cut(s) 203, 1527, 2436, 2460, 2982
SacI GAGCTC 4 cut(s) 627, 804, 1011, 1761
SacII CCGCGG 1 cut(s) 1049
SalI GTCGAC 3 cut(s) 218, 702, 2225
SaqAI TTAA 3 cut(s) 635, 738, 2928
Sau3AI GATC 7 cut(s) 207, 612, 1161, 1660, 1716, 2749, 2974
SchI GAGTC 9 cut(s) 119, 482, 686, 699, 910, 983, 1132, 1204, 1567
SduI GDGCHC 5 cut(s) 627, 804, 1011, 1761, 2835
SfaNI GCATC 7 cut(s) 189, 1143, 2392, 2530, 2926, 2956, 2978
SfcI CTRYAG 3 cut(s) 1436, 1980, 2647
Sfr274I CTCGAG 3 cut(s) 497, 644, 1298
Sfr303I CCGCGG 1 cut(s) 1049
SfuI TTCGAA 1 cut(s) 1392
SgrAI CRCCGGYG 1 cut(s) 1034
SgrBI CCGCGG 1 cut(s) 1049
SinI GGWCC 7 cut(s) 502, 649, 727, 1516, 2197, 2764, 2818
SlaI CTCGAG 3 cut(s) 497, 644, 1298
SmaI CCCGGG 1 cut(s) 606
SmiMI CAYNNNNRTG 5 cut(s) 203, 1527, 2436, 2460, 2982
SmlI CTYRAG 3 cut(s) 497, 644, 1298
SmoI CTYRAG 3 cut(s) 497, 644, 1298
SseBI AGGCCT 2 cut(s) 84, 828
SspMI CTAG 2 cut(s) 1266, 2286
SstI GAGCTC 4 cut(s) 627, 804, 1011, 1761
StuI AGGCCT 2 cut(s) 84, 828
StyI CCWWGG 3 cut(s) 1776, 1911, 2745
TaaI ACNGT 2 cut(s) 1608, 2651
TaiI ACGT 3 cut(s) 207, 344, 1225
TaqII GACCGA 3 cut(s) 677, 788, 2560
TatI WGTACW 4 cut(s) 1785, 1950, 2097, 2313
TauI GCSGC 6 cut(s) 270, 835, 1048, 1072, 1285, 1355
TfiI GAWTC 9 cut(s) 486, 757, 901, 940, 1051, 1186, 1672, 2665, 2789
Tru1I TTAA 3 cut(s) 635, 738, 2928
Tru9I TTAA 3 cut(s) 635, 738, 2928
TscAI CASTG 4 cut(s) 1456, 1471, 2139, 2625
TseFI GTSAC 4 cut(s) 1235, 1451, 2639, 2946
TseI GCWGC 6 cut(s) 865, 1042, 1279, 1907, 2344, 2405
Tsp45I GTSAC 4 cut(s) 1235, 1451, 2639, 2946
TspDTI ATGAA 7 cut(s) 17, 896, 1727, 1888, 2073, 2444, 2976
TspGWI ACGGA 2 cut(s) 205, 2924
TspMI CCCGGG 1 cut(s) 604
TspRI CASTG 4 cut(s) 1456, 1471, 2139, 2625
Tth111I GACNNNGTC 1 cut(s) 2768
VpaK11BI GGWCC 7 cut(s) 502, 649, 727, 1516, 2197, 2764, 2818
XapI RAATTY 3 cut(s) 465, 1588, 2705
XceI RCATGY 4 cut(s) 189, 1289, 1642, 2320
XhoI CTCGAG 3 cut(s) 497, 644, 1298
XmaI CCCGGG 1 cut(s) 604
XmiI GTMKAC 4 cut(s) 219, 516, 703, 2226
XmnI GAANNNNTTC 3 cut(s) 956, 1388, 1431
XspI CTAG 2 cut(s) 1266, 2286
Zsp2I ATGCAT 2 cut(s) 2614, 2971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.