MD05G1329000.v1.1

Nitric oxide synthase-interacting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013491
Physical Location & Seq
Reverse (-)
45414596 .. 45418508
3913 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1329000.v1.1.491

Sequence Viewer

Length: 912 bp
ATGCCGCAGAGGCACTCGAAGAACAACAACGACCTCGCTTTCTTCACCTACGACGAGAAGAAGAAGCTCGGCTATGGAACCCAGAGGGAGCGGCTCGGCAGGGACTCCATCAAGCCCTTCGACGCCTGCTGCCTCTGCCTCAAGCCCTTCATCGACCCCATGTGCTGCCAGAAAGGCCACGTCTTTTGCAAGGAATGCATTCTCGAGTGCTTGCTTTCTCAGAAGAAGGACATCCACAGAAAGCTAGCTGCACATACTGCTCAGCAGAAGCAAGAAAAGAACGAGGAAGATGATCGGTTAATGCAGCAGAAAGCTAGAGAGCTTGAGGCTTTTGATCAGCAAAACCACGGCGCAATTCCACAATACAATGATAGAAACCAGAGCCAAGATAAGACTGGTTTCCACGGGGCAAACAGTGTGAAGGTCACTTCTTATGAAGAGGAGGCTCTTCGGACAATGAAAGCCTTTTGGCTGCCTTCTGCTACACCGGCAGCTCCTGTTAAAGTAGATGCTCCCTCTATGAGCACGGTCTGTCCAGAAGGAAATGAGAAACTAAAGCTGAAGTCCCTATTCTCTGTGTATTACACAGAAGACAGTAACGAGAAGAAGACTTCATCTCTGGATAAGACACTTATCTGTCCGAGCTGTAAGGTTACTTTGACCAACACGCTGTCGCTCGTGGCCCTTAGTTCATGCGGGCATGTCTTTTGTAAGAAATGTGCTGATAAGTTCATGGCCGTAGATAAGGTTTGCCTTGTTTGCAGCAAGGGATGCAAAGAAAGGAACTTGGTGAACTTGGCAAAAGGAGGAACCGGCTTTGCCGGGCACGATGATAATCTTGAAGCAAGAGACTTCAAGCATTTGGGGAGTGGTTCCGGATTAGGGTTGGTGAGACCCGCCGCGAAGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rtf2 zf-NOSIP zf-RING_UBOX

Protein Analysis

304

Amino Acids

33.73

Weight (kDa)

8.73

Isoelectric Point (pI)

37.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-NOSIP PF15906 5 - 75 2.2e-15 Zinc-finger of nitric oxide synthase-interacting protein
Rtf2 PF04641 192 - 268 1.7e-06 Rtf2 RING-finger
zf-RING_5 PF14634 213 - 256 2.5e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013491)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61620
fragaria_vesca FvH4_3g03660
malus_domestica MD05G1329000.v1.1 MD10G1305900.v1.1
prunus_persica Prupe.4G033800_v2.0.a1
pyrus_communis pycom05g30230 pycom10g25820
rosa_chinensis RchiOBHm_Chr5g0005701
rosa_laevigata RLG00000031319
rosa_multiflora Rmu_sc0010248.1_g000004
rosa_roxburghii Rroxscaffold_1G00070140
rosa_rugosa Rorug04G0417400
rosa_samantha Rh5AG045000 Rh5BG043700 Rh5CG052500 Rh5DG043500
rosa_wichuraiana Rw0G008370 Rw5G004520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 91
AccII CGCG 1 cut(s) 902
AccIII TCCGGA 1 cut(s) 875
AciI CCGC 5 cut(s) 5, 91, 696, 897, 900
AcoI YGGCCR 1 cut(s) 735
AcuI CTGAAG 1 cut(s) 581
AcyI GRCGYC 1 cut(s) 123
AfiI CCNNNNNNNGG 1 cut(s) 882
AgsI TTSAA 2 cut(s) 842, 856
AjiI CACGTC 1 cut(s) 181
AluBI AGCT 8 cut(s) 67, 244, 248, 314, 322, 494, 559, 645
AluI AGCT 8 cut(s) 67, 244, 248, 314, 322, 494, 559, 645
Alw21I GWGCWC 1 cut(s) 527
Alw26I GTCTC 2 cut(s) 843, 886
AlwNI CAGNNNCTG 1 cut(s) 497
Ama87I CYCGRG 1 cut(s) 203
Aor13HI TCCGGA 1 cut(s) 875
AoxI GGCC 3 cut(s) 175, 681, 735
ApeKI GCWGC 7 cut(s) 129, 165, 248, 304, 472, 491, 762
Asp700I GAANNNNTTC 1 cut(s) 198
AspLEI GCGC 1 cut(s) 353
AspS9I GGNCC 1 cut(s) 682
AsuC2I CCSGG 1 cut(s) 823
AsuHPI GGTGA 3 cut(s) 37, 802, 901
AsuNHI GCTAGC 1 cut(s) 244
AvaI CYCGRG 1 cut(s) 203
BaeGI GKGCMC 1 cut(s) 828
BauI CACGAG 1 cut(s) 677
BbsI GAAGAC 2 cut(s) 597, 614
Bbv12I GWGCWC 1 cut(s) 527
BbvI GCAGC 7 cut(s) 116, 152, 235, 316, 459, 503, 774
BccI CCATC 1 cut(s) 116
BceAI ACGGC 2 cut(s) 364, 722
BclI TGATCA 1 cut(s) 334
BcnI CCSGG 1 cut(s) 823
BcoDI GTCTC 2 cut(s) 843, 886
BfaI CTAG 2 cut(s) 245, 315
BglI GCCNNNNNGGC 2 cut(s) 10, 174
BlpI GCTNAGC 1 cut(s) 261
Bme1390I CCNGG 1 cut(s) 823
BmeT110I CYCGRG 1 cut(s) 203
BmgBI CACGTC 1 cut(s) 181
BmgT120I GGNCC 1 cut(s) 682
BmiI GGNNCC 3 cut(s) 79, 811, 874
BmrFI CCNGG 1 cut(s) 823
BmsI GCATC 2 cut(s) 499, 761
BmtI GCTAGC 1 cut(s) 248
BpiI GAAGAC 2 cut(s) 597, 614
Bpu1102I GCTNAGC 1 cut(s) 261
BpuEI CTTGAG 2 cut(s) 125, 344
BpuMI CCSGG 1 cut(s) 823
BsaBI GATNNNNATC 1 cut(s) 834
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 886
BsaJI CCNNGG 2 cut(s) 346, 403
BsaWI WCCGGW 1 cut(s) 875
Bsc4I CCNNNNNNNGG 1 cut(s) 882
Bse118I RCCGGY 2 cut(s) 487, 812
Bse1I ACTGG 1 cut(s) 400
Bse8I GATNNNNATC 1 cut(s) 834
BseAI TCCGGA 1 cut(s) 875
BseDI CCNNGG 2 cut(s) 346, 403
BseGI GGATG 2 cut(s) 231, 776
BseJI GATNNNNATC 1 cut(s) 834
BseLI CCNNNNNNNGG 1 cut(s) 882
BseMII CTCAG 2 cut(s) 233, 275
BseNI ACTGG 1 cut(s) 400
BseRI GAGGAG 1 cut(s) 455
BseSI GKGCMC 1 cut(s) 828
BseXI GCAGC 7 cut(s) 116, 152, 235, 316, 459, 503, 774
BsgI GTGCAG 1 cut(s) 234
Bsh1236I CGCG 1 cut(s) 902
BshFI GGCC 3 cut(s) 177, 683, 737
BsiHKAI GWGCWC 1 cut(s) 527
BsiHKCI CYCGRG 1 cut(s) 203
BsiSI CCGG 4 cut(s) 488, 813, 822, 876
BslFI GGGAC 2 cut(s) 116, 550
BslI CCNNNNNNNGG 1 cut(s) 882
BsmAI GTCTC 2 cut(s) 843, 886
BsmFI GGGAC 2 cut(s) 116, 550
BsmI GAATGC 2 cut(s) 198, 200
BsnI GGCC 3 cut(s) 177, 683, 737
Bso31I GGTCTC 1 cut(s) 886
BsoBI CYCGRG 1 cut(s) 203
Bsp1286I GDGCHC 2 cut(s) 527, 828
Bsp13I TCCGGA 1 cut(s) 875
Bsp143I GATC 2 cut(s) 292, 334
Bsp1720I GCTNAGC 1 cut(s) 261
BspACI CCGC 5 cut(s) 5, 91, 696, 897, 900
BspANI GGCC 3 cut(s) 177, 683, 737
BspCNI CTCAG 2 cut(s) 232, 274
BspEI TCCGGA 1 cut(s) 875
BspFNI CGCG 1 cut(s) 902
BspLI GGNNCC 3 cut(s) 79, 811, 874
BspOI GCTAGC 1 cut(s) 248
BspQI GCTCTTC 1 cut(s) 453
BspTNI GGTCTC 1 cut(s) 886
BsrBI CCGCTC 1 cut(s) 91
BsrFI RCCGGY 2 cut(s) 487, 812
BsrI ACTGG 1 cut(s) 400
BssAI RCCGGY 2 cut(s) 487, 812
BssECI CCNNGG 2 cut(s) 346, 403
BssMI GATC 2 cut(s) 292, 334
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 1 cut(s) 677
Bst2BI CACGAG 1 cut(s) 677
Bst4CI ACNGT 3 cut(s) 416, 529, 596
Bst6I CTCTTC 2 cut(s) 432, 453
BstACI GRCGYC 1 cut(s) 123
BstAPI GCANNNNNTGC 3 cut(s) 195, 257, 771
BstC8I GCNNGC 4 cut(s) 127, 212, 246, 698
BstDEI CTNAG 3 cut(s) 219, 261, 686
BstDSI CCRYGG 2 cut(s) 346, 403
BstF5I GGATG 2 cut(s) 231, 776
BstFNI CGCG 1 cut(s) 902
BstHHI GCGC 1 cut(s) 353
BstKTI GATC 2 cut(s) 295, 337
BstMAI GTCTC 2 cut(s) 843, 886
BstMBI GATC 2 cut(s) 292, 334
BstMWI GCNNNNNNNGC 8 cut(s) 10, 135, 174, 195, 257, 488, 759, 771
BstNSI RCATGY 1 cut(s) 704
BstSCI CCNGG 1 cut(s) 821
BstSLI GKGCMC 1 cut(s) 828
BstUI CGCG 1 cut(s) 902
BstV1I GCAGC 7 cut(s) 116, 152, 235, 316, 459, 503, 774
BstV2I GAAGAC 2 cut(s) 597, 614
BsuRI GGCC 3 cut(s) 177, 683, 737
BtgI CCRYGG 2 cut(s) 346, 403
BtrI CACGTC 1 cut(s) 181
BtsCI GGATG 2 cut(s) 231, 776
BtsIMutI CAGTG 1 cut(s) 421
Cac8I GCNNGC 4 cut(s) 127, 212, 246, 698
CaiI CAGNNNCTG 1 cut(s) 497
CfoI GCGC 1 cut(s) 353
Cfr10I RCCGGY 2 cut(s) 487, 812
Cfr13I GGNCC 1 cut(s) 682
CseI GACGC 1 cut(s) 131
CspCI CAANNNNNGTGG 2 cut(s) 167, 202
CviAII CATG 4 cut(s) 160, 693, 701, 733
DdeI CTNAG 3 cut(s) 219, 261, 686
DpnI GATC 2 cut(s) 294, 336
DpnII GATC 2 cut(s) 292, 334
EaeI YGGCCR 1 cut(s) 735
Eam1104I CTCTTC 2 cut(s) 432, 453
EarI CTCTTC 2 cut(s) 432, 453
Eco31I GGTCTC 1 cut(s) 886
Eco57I CTGAAG 1 cut(s) 581
Eco88I CYCGRG 1 cut(s) 203
EcoT22I ATGCAT 1 cut(s) 200
FaeI CATG 4 cut(s) 163, 696, 704, 736
FaiI YATR 8 cut(s) 75, 161, 255, 435, 521, 694, 702, 734
FaqI GGGAC 2 cut(s) 116, 550
FatI CATG 4 cut(s) 159, 692, 700, 732
FauI CCCGC 2 cut(s) 689, 904
FbaI TGATCA 1 cut(s) 334
FokI GGATG 2 cut(s) 218, 783
FspBI CTAG 2 cut(s) 245, 315
GlaI GCGC 1 cut(s) 352
HaeIII GGCC 3 cut(s) 177, 683, 737
HapII CCGG 4 cut(s) 488, 813, 822, 876
HgaI GACGC 1 cut(s) 131
HhaI GCGC 1 cut(s) 353
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 4 cut(s) 163, 696, 704, 736
Hin6I GCGC 1 cut(s) 351
HinP1I GCGC 1 cut(s) 351
HinfI GANTC 1 cut(s) 104
HpaII CCGG 4 cut(s) 488, 813, 822, 876
HphI GGTGA 3 cut(s) 37, 802, 901
Hpy166II GTNNAC 1 cut(s) 793
Hpy188I TCNGA 3 cut(s) 222, 453, 642
Hpy188III TCNNGA 5 cut(s) 203, 536, 620, 839, 876
Hpy8I GTNNAC 1 cut(s) 793
Hpy99I CGWCG 2 cut(s) 56, 125
HpyAV CCTTC 6 cut(s) 127, 157, 220, 415, 486, 533
HpyCH4III ACNGT 3 cut(s) 416, 529, 596
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 6 cut(s) 189, 198, 251, 304, 762, 774
HpyF10VI GCNNNNNNNGC 8 cut(s) 10, 135, 174, 195, 257, 488, 759, 771
HpyF3I CTNAG 3 cut(s) 219, 261, 686
HpySE526I ACGT 1 cut(s) 180
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 4 cut(s) 163, 696, 704, 736
HspAI GCGC 1 cut(s) 351
Kpn2I TCCGGA 1 cut(s) 875
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 2 cut(s) 292, 334
LguI GCTCTTC 1 cut(s) 453
LmnI GCTCC 3 cut(s) 88, 499, 517
Lsp1109I GCAGC 7 cut(s) 116, 152, 235, 316, 459, 503, 774
LweI GCATC 2 cut(s) 499, 761
MaeI CTAG 2 cut(s) 245, 315
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 3 cut(s) 424, 596, 652
MalI GATC 2 cut(s) 294, 336
MbiI CCGCTC 1 cut(s) 91
MboI GATC 2 cut(s) 292, 334
MhlI GDGCHC 2 cut(s) 527, 828
MluCI AATT 1 cut(s) 354
MlyI GAGTC 1 cut(s) 98
Mph1103I ATGCAT 1 cut(s) 200
MroI TCCGGA 1 cut(s) 875
MroXI GAANNNNTTC 1 cut(s) 198
MseI TTAA 3 cut(s) 299, 501, 910
MspI CCGG 4 cut(s) 488, 813, 822, 876
MspR9I CCNGG 1 cut(s) 823
Mva1269I GAATGC 2 cut(s) 198, 200
MvnI CGCG 1 cut(s) 902
MwoI GCNNNNNNNGC 8 cut(s) 10, 135, 174, 195, 257, 488, 759, 771
NciI CCSGG 1 cut(s) 823
NdeII GATC 2 cut(s) 292, 334
NheI GCTAGC 1 cut(s) 244
NlaIII CATG 4 cut(s) 163, 696, 704, 736
NlaIV GGNNCC 3 cut(s) 79, 811, 874
NmeAIII GCCGAG 2 cut(s) 48, 75
NmuCI GTSAC 1 cut(s) 424
NsiI ATGCAT 1 cut(s) 200
NspI RCATGY 1 cut(s) 704
PaeR7I CTCGAG 1 cut(s) 203
PciSI GCTCTTC 1 cut(s) 453
PctI GAATGC 2 cut(s) 198, 200
PdmI GAANNNNTTC 1 cut(s) 198
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
PspN4I GGNNCC 3 cut(s) 79, 811, 874
PspPI GGNCC 1 cut(s) 682
PstNI CAGNNNCTG 1 cut(s) 497
SapI GCTCTTC 1 cut(s) 453
SaqAI TTAA 3 cut(s) 299, 501, 910
Sau3AI GATC 2 cut(s) 292, 334
Sau96I GGNCC 1 cut(s) 682
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 1 cut(s) 823
SduI GDGCHC 2 cut(s) 527, 828
SfaNI GCATC 2 cut(s) 499, 761
Sfr274I CTCGAG 1 cut(s) 203
SlaI CTCGAG 1 cut(s) 203
SmlI CTYRAG 3 cut(s) 140, 203, 323
SmoI CTYRAG 3 cut(s) 140, 203, 323
Sse9I AATT 1 cut(s) 354
SsiI CCGC 5 cut(s) 5, 91, 696, 897, 900
SspMI CTAG 2 cut(s) 245, 315
StyD4I CCNGG 1 cut(s) 821
TaaI ACNGT 3 cut(s) 416, 529, 596
TaiI ACGT 1 cut(s) 183
TaqI TCGA 4 cut(s) 17, 120, 153, 204
TasI AATT 1 cut(s) 354
TauI GCSGC 3 cut(s) 7, 94, 902
Tru1I TTAA 3 cut(s) 299, 501, 910
Tru9I TTAA 3 cut(s) 299, 501, 910
TscAI CASTG 1 cut(s) 421
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 7 cut(s) 129, 165, 248, 304, 472, 491, 762
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 6 cut(s) 139, 450, 473, 603, 681, 721
TspRI CASTG 1 cut(s) 421
XceI RCATGY 1 cut(s) 704
XcmI CCANNNNNNNNNTGG 1 cut(s) 392
XhoI CTCGAG 1 cut(s) 203
XmnI GAANNNNTTC 1 cut(s) 198
XspI CTAG 2 cut(s) 245, 315
Zsp2I ATGCAT 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.