Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0013491

Physical Location & Seq

Genomic Coordinates Reverse (-)

39182749 .. 39194043

Gene Structure 11295 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1305900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 915 bp

ATGCCGCAGAGGCACTCGAAGAACAACAACGACCTCGCTTTCTTCACCTACGACGAGAAGAAGAAGCTCGGCTATGGAACCCAGAGGGAGCGGTTGGGCAGGGACTCCATCAAGCCCTTCGACGCCTGCTGCCTCTGCCTGAAACCCTTCATCGACCCCATGTGCTGCCAGAAAGGCCACGTCTTTTGCAAGGAATGCATTCTCGAGTGCTTGCTTTCTCAGAAGAAGGATATCCATAGAAAGCTAGCTGCACATACTGCTCAGCAGAAGCAAGAAAAGAACGAGGAAGAAGATAGGCTTGTGCAGCAGAAAGCTAGAGAGCTTGAGGCTTTTGATCAGCAAAACCATGGTGCAATTCCACAAAACAATGATAGAAACCAGAGCCAAGATAAGACAGGTTTCCATGGGGCGAATAGTGTGAAGGTCACTTCTTATGAAGAGGAGGCTCTTCGGACAATGAAAGCCTTTTGGCTGCCTTCTGCTACACCGGCAGCTCCTGTTAAAGTAGATGCTCCCTCTACGAGCACGGTCTGTCCGGAAGGAAATGAGAAACTAACGCTGAAGTCCCTATTCTCTGTGTACTACACAGAAGACAATAACGAGAAGAAGAAGAAATCATCTCTGGATAAGACATTTATATGTCCGAGCTGCAAGGTTACTTTGACCAACACGCTGTCGCTCGTGGCCCTTAGTTCATGCGGGCATGTCTTTTGTAAGAAATGTGCCGATAGGTTCATGGCTGTAGATAAGGTTTGCCTCGTTTGCAGCAAGGGATGCAAGGAAAGGAACTTGGTGAACTTGGCAAAAGGAGGAACAGGCTTTGCAGGGCACGATGATAATCTTGAAGCAAGAGACTTTAAGCATTTGGGGAGCGGTTCCGGATTAGGGTTGGTGAGGCCTGCCGTGAAGACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009791 GO:0009987 GO:0016567 GO:0016604 GO:0016607 GO:0016740 GO:0019538 GO:0019787 GO:0031647 GO:0031648 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0048856 GO:0050896 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070013 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Rtf2 zf-NOSIP zf-RING_UBOX

Protein Analysis

305

Amino Acids

33.87

Weight (kDa)

8.81

Isoelectric Point (pI)

37.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-NOSIP	PF15906	5 - 75	2.2e-15	Zinc-finger of nitric oxide synthase-interacting protein
Rtf2	PF04641	188 - 269	2.9e-08	Rtf2 RING-finger
zf-RING_5	PF14634	214 - 257	2.5e-06	zinc-RING finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013491)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G61620
fragaria_vesca	FvH4_3g03660
malus_domestica	MD05G1329000.v1.1 MD10G1305900.v1.1
prunus_persica	Prupe.4G033800_v2.0.a1
pyrus_communis	pycom05g30230 pycom10g25820
rosa_chinensis	RchiOBHm_Chr5g0005701
rosa_laevigata	RLG00000031319
rosa_multiflora	Rmu_sc0010248.1_g000004
rosa_roxburghii	Rroxscaffold_1G00070140
rosa_rugosa	Rorug04G0417400
rosa_samantha	Rh5AG045000 Rh5BG043700 Rh5CG052500 Rh5DG043500
rosa_wichuraiana	Rw0G008370 Rw5G004520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	2 cut(s)	91, 873
AccIII	TCCGGA	2 cut(s)	535, 878
AciI	CCGC	4 cut(s)	5, 91, 699, 873
AcuI	CTGAAG	1 cut(s)	581
AcyI	GRCGYC	1 cut(s)	123
AfaI	GTAC	1 cut(s)	581
AfiI	CCNNNNNNNGG	1 cut(s)	885
AgsI	TTSAA	1 cut(s)	845
AjiI	CACGTC	1 cut(s)	181
AluBI	AGCT	7 cut(s)	67, 244, 248, 314, 322, 494, 648
AluI	AGCT	7 cut(s)	67, 244, 248, 314, 322, 494, 648
Alw21I	GWGCWC	1 cut(s)	527
Alw26I	GTCTC	1 cut(s)	846
AlwNI	CAGNNNCTG	1 cut(s)	497
Ama87I	CYCGRG	1 cut(s)	203
Aor13HI	TCCGGA	2 cut(s)	535, 878
AoxI	GGCC	3 cut(s)	175, 684, 896
ApeKI	GCWGC	8 cut(s)	129, 165, 248, 304, 472, 491, 648, 765
Asp700I	GAANNNNTTC	2 cut(s)	146, 198
AspS9I	GGNCC	1 cut(s)	685
AsuHPI	GGTGA	3 cut(s)	37, 805, 904
AsuNHI	GCTAGC	1 cut(s)	244
AvaI	CYCGRG	1 cut(s)	203
BaeGI	GKGCMC	1 cut(s)	831
BauI	CACGAG	1 cut(s)	680
BbsI	GAAGAC	1 cut(s)	597
Bbv12I	GWGCWC	1 cut(s)	527
BbvI	GCAGC	8 cut(s)	116, 152, 235, 316, 459, 503, 635, 777
BccI	CCATC	1 cut(s)	116
BceAI	ACGGC	1 cut(s)	887
BclI	TGATCA	1 cut(s)	334
BcoDI	GTCTC	1 cut(s)	846
BfaI	CTAG	2 cut(s)	245, 315
BfmI	CTRYAG	1 cut(s)	741
BglI	GCCNNNNNGGC	2 cut(s)	10, 174
BisI	GCNGC	9 cut(s)	5, 130, 166, 249, 305, 473, 492, 649, 766
BlpI	GCTNAGC	1 cut(s)	261
BlsI	GCNGC	9 cut(s)	6, 131, 167, 250, 306, 474, 493, 650, 767
BmeT110I	CYCGRG	1 cut(s)	203
BmgBI	CACGTC	1 cut(s)	181
BmgT120I	GGNCC	1 cut(s)	685
BmiI	GGNNCC	2 cut(s)	79, 877
BmsI	GCATC	2 cut(s)	499, 764
BmtI	GCTAGC	1 cut(s)	248
BpiI	GAAGAC	1 cut(s)	597
Bpu1102I	GCTNAGC	1 cut(s)	261
BpuEI	CTTGAG	1 cut(s)	344
BsaBI	GATNNNNATC	1 cut(s)	837
BsaHI	GRCGYC	1 cut(s)	123
BsaJI	CCNNGG	2 cut(s)	346, 403
BsaWI	WCCGGW	2 cut(s)	535, 878
Bsc4I	CCNNNNNNNGG	1 cut(s)	885
Bse118I	RCCGGY	1 cut(s)	487
Bse8I	GATNNNNATC	1 cut(s)	837
BseAI	TCCGGA	2 cut(s)	535, 878
BseDI	CCNNGG	2 cut(s)	346, 403
BseGI	GGATG	1 cut(s)	779
BseJI	GATNNNNATC	1 cut(s)	837
BseLI	CCNNNNNNNGG	1 cut(s)	885
BseMII	CTCAG	2 cut(s)	233, 275
BseRI	GAGGAG	1 cut(s)	455
BseSI	GKGCMC	1 cut(s)	831
BseXI	GCAGC	8 cut(s)	116, 152, 235, 316, 459, 503, 635, 777
BsgI	GTGCAG	2 cut(s)	234, 323
BshFI	GGCC	3 cut(s)	177, 686, 898
BsiHKAI	GWGCWC	1 cut(s)	527
BsiHKCI	CYCGRG	1 cut(s)	203
BsiSI	CCGG	3 cut(s)	488, 536, 879
BslFI	GGGAC	2 cut(s)	116, 550
BslI	CCNNNNNNNGG	1 cut(s)	885
BsmAI	GTCTC	1 cut(s)	846
BsmFI	GGGAC	2 cut(s)	116, 550
BsmI	GAATGC	2 cut(s)	198, 200
BsnI	GGCC	3 cut(s)	177, 686, 898
BsoBI	CYCGRG	1 cut(s)	203
Bsp1286I	GDGCHC	2 cut(s)	527, 831
Bsp13I	TCCGGA	2 cut(s)	535, 878
Bsp143I	GATC	1 cut(s)	334
Bsp1720I	GCTNAGC	1 cut(s)	261
Bsp19I	CCATGG	2 cut(s)	346, 403
BspACI	CCGC	4 cut(s)	5, 91, 699, 873
BspANI	GGCC	3 cut(s)	177, 686, 898
BspCNI	CTCAG	2 cut(s)	232, 274
BspEI	TCCGGA	2 cut(s)	535, 878
BspLI	GGNNCC	2 cut(s)	79, 877
BspOI	GCTAGC	1 cut(s)	248
BspQI	GCTCTTC	1 cut(s)	453
BsrBI	CCGCTC	2 cut(s)	91, 873
BsrFI	RCCGGY	1 cut(s)	487
BssAI	RCCGGY	1 cut(s)	487
BssECI	CCNNGG	2 cut(s)	346, 403
BssMI	GATC	1 cut(s)	334
BssNI	GRCGYC	1 cut(s)	123
BssSI	CACGAG	1 cut(s)	680
BssT1I	CCWWGG	2 cut(s)	346, 403
Bst2BI	CACGAG	1 cut(s)	680
Bst4CI	ACNGT	1 cut(s)	529
Bst6I	CTCTTC	2 cut(s)	432, 453
BstACI	GRCGYC	1 cut(s)	123
BstAPI	GCANNNNNTGC	3 cut(s)	195, 257, 774
BstC8I	GCNNGC	5 cut(s)	127, 212, 246, 701, 900
BstDEI	CTNAG	3 cut(s)	219, 261, 689
BstDSI	CCRYGG	2 cut(s)	346, 403
BstF5I	GGATG	1 cut(s)	779
BstKTI	GATC	1 cut(s)	337
BstMAI	GTCTC	1 cut(s)	846
BstMBI	GATC	1 cut(s)	334
BstMWI	GCNNNNNNNGC	9 cut(s)	10, 135, 174, 195, 257, 304, 488, 762, 774
BstNSI	RCATGY	1 cut(s)	707
BstSFI	CTRYAG	1 cut(s)	741
BstSLI	GKGCMC	1 cut(s)	831
BstV1I	GCAGC	8 cut(s)	116, 152, 235, 316, 459, 503, 635, 777
BstV2I	GAAGAC	1 cut(s)	597
BsuRI	GGCC	3 cut(s)	177, 686, 898
BtgI	CCRYGG	2 cut(s)	346, 403
BtrI	CACGTC	1 cut(s)	181
BtsCI	GGATG	1 cut(s)	779
Cac8I	GCNNGC	5 cut(s)	127, 212, 246, 701, 900
CaiI	CAGNNNCTG	1 cut(s)	497
Cfr10I	RCCGGY	1 cut(s)	487
Cfr13I	GGNCC	1 cut(s)	685
CseI	GACGC	1 cut(s)	131
Csp6I	GTAC	1 cut(s)	580
CspCI	CAANNNNNGTGG	2 cut(s)	167, 202
CviAII	CATG	6 cut(s)	160, 347, 404, 696, 704, 736
CviQI	GTAC	1 cut(s)	580
DdeI	CTNAG	3 cut(s)	219, 261, 689
DpnI	GATC	1 cut(s)	336
DpnII	GATC	1 cut(s)	334
Eam1104I	CTCTTC	2 cut(s)	432, 453
EarI	CTCTTC	2 cut(s)	432, 453
Eco130I	CCWWGG	2 cut(s)	346, 403
Eco147I	AGGCCT	1 cut(s)	898
Eco32I	GATATC	1 cut(s)	232
Eco57I	CTGAAG	1 cut(s)	581
Eco88I	CYCGRG	1 cut(s)	203
EcoRV	GATATC	1 cut(s)	232
EcoT14I	CCWWGG	2 cut(s)	346, 403
EcoT22I	ATGCAT	1 cut(s)	200
ErhI	CCWWGG	2 cut(s)	346, 403
FaeI	CATG	6 cut(s)	163, 350, 407, 699, 707, 739
FalI	AAGNNNNNCTT	2 cut(s)	282, 314
FaqI	GGGAC	2 cut(s)	116, 550
FatI	CATG	6 cut(s)	159, 346, 403, 695, 703, 735
FauI	CCCGC	1 cut(s)	692
FbaI	TGATCA	1 cut(s)	334
Fnu4HI	GCNGC	9 cut(s)	5, 130, 166, 249, 305, 473, 492, 649, 766
FokI	GGATG	1 cut(s)	786
Fsp4HI	GCNGC	9 cut(s)	5, 130, 166, 249, 305, 473, 492, 649, 766
FspBI	CTAG	2 cut(s)	245, 315
GluI	GCNGC	9 cut(s)	5, 130, 166, 249, 305, 473, 492, 649, 766
HaeIII	GGCC	3 cut(s)	177, 686, 898
HapII	CCGG	3 cut(s)	488, 536, 879
HgaI	GACGC	1 cut(s)	131
Hin1I	GRCGYC	1 cut(s)	123
Hin1II	CATG	6 cut(s)	163, 350, 407, 699, 707, 739
HinfI	GANTC	1 cut(s)	104
HpaII	CCGG	3 cut(s)	488, 536, 879
HphI	GGTGA	3 cut(s)	37, 805, 904
Hpy166II	GTNNAC	2 cut(s)	580, 796
Hpy188I	TCNGA	3 cut(s)	222, 453, 645
Hpy188III	TCNNGA	5 cut(s)	203, 536, 623, 842, 879
Hpy8I	GTNNAC	2 cut(s)	580, 796
Hpy99I	CGWCG	2 cut(s)	56, 125
HpyAV	CCTTC	6 cut(s)	127, 157, 220, 415, 486, 533
HpyCH4III	ACNGT	1 cut(s)	529
HpyCH4IV	ACGT	1 cut(s)	180
HpyCH4V	TGCA	9 cut(s)	189, 198, 251, 304, 353, 651, 765, 777, 824
HpyF10VI	GCNNNNNNNGC	9 cut(s)	10, 135, 174, 195, 257, 304, 488, 762, 774
HpyF3I	CTNAG	3 cut(s)	219, 261, 689
HpySE526I	ACGT	1 cut(s)	180
Hsp92I	GRCGYC	1 cut(s)	123
Hsp92II	CATG	6 cut(s)	163, 350, 407, 699, 707, 739
Kpn2I	TCCGGA	2 cut(s)	535, 878
Ksp22I	TGATCA	1 cut(s)	334
Kzo9I	GATC	1 cut(s)	334
LguI	GCTCTTC	1 cut(s)	453
LmnI	GCTCC	4 cut(s)	88, 499, 517, 870
Lsp1109I	GCAGC	8 cut(s)	116, 152, 235, 316, 459, 503, 635, 777
LweI	GCATC	2 cut(s)	499, 764
MaeI	CTAG	2 cut(s)	245, 315
MaeII	ACGT	1 cut(s)	180
MaeIII	GTNAC	2 cut(s)	424, 655
MalI	GATC	1 cut(s)	336
MbiI	CCGCTC	2 cut(s)	91, 873
MboI	GATC	1 cut(s)	334
MhlI	GDGCHC	2 cut(s)	527, 831
MluCI	AATT	1 cut(s)	354
MlyI	GAGTC	1 cut(s)	98
Mph1103I	ATGCAT	1 cut(s)	200
MroI	TCCGGA	2 cut(s)	535, 878
MroXI	GAANNNNTTC	2 cut(s)	146, 198
MseI	TTAA	3 cut(s)	501, 858, 913
MslI	CAYNNNNRTG	1 cut(s)	637
MspI	CCGG	3 cut(s)	488, 536, 879
Mva1269I	GAATGC	2 cut(s)	198, 200
MwoI	GCNNNNNNNGC	9 cut(s)	10, 135, 174, 195, 257, 304, 488, 762, 774
NcoI	CCATGG	2 cut(s)	346, 403
NdeII	GATC	1 cut(s)	334
NheI	GCTAGC	1 cut(s)	244
NlaIII	CATG	6 cut(s)	163, 350, 407, 699, 707, 739
NlaIV	GGNNCC	2 cut(s)	79, 877
NmeAIII	GCCGAG	1 cut(s)	48
NmuCI	GTSAC	1 cut(s)	424
NsiI	ATGCAT	1 cut(s)	200
NspI	RCATGY	1 cut(s)	707
PaeR7I	CTCGAG	1 cut(s)	203
PceI	AGGCCT	1 cut(s)	898
PciSI	GCTCTTC	1 cut(s)	453
PctI	GAATGC	2 cut(s)	198, 200
PdmI	GAANNNNTTC	2 cut(s)	146, 198
PkrI	GCNGC	9 cut(s)	6, 131, 167, 250, 306, 474, 493, 650, 767
PleI	GAGTC	1 cut(s)	98
PpsI	GAGTC	1 cut(s)	98
PspN4I	GGNNCC	2 cut(s)	79, 877
PspPI	GGNCC	1 cut(s)	685
PstNI	CAGNNNCTG	1 cut(s)	497
RsaI	GTAC	1 cut(s)	581
RsaNI	GTAC	1 cut(s)	580
RseI	CAYNNNNRTG	1 cut(s)	637
SapI	GCTCTTC	1 cut(s)	453
SaqAI	TTAA	3 cut(s)	501, 858, 913
SatI	GCNGC	9 cut(s)	5, 130, 166, 249, 305, 473, 492, 649, 766
Sau3AI	GATC	1 cut(s)	334
Sau96I	GGNCC	1 cut(s)	685
SchI	GAGTC	1 cut(s)	98
SduI	GDGCHC	2 cut(s)	527, 831
SfaNI	GCATC	2 cut(s)	499, 764
SfcI	CTRYAG	1 cut(s)	741
Sfr274I	CTCGAG	1 cut(s)	203
SlaI	CTCGAG	1 cut(s)	203
SmiMI	CAYNNNNRTG	1 cut(s)	637
SmlI	CTYRAG	2 cut(s)	203, 323
SmoI	CTYRAG	2 cut(s)	203, 323
Sse9I	AATT	1 cut(s)	354
SseBI	AGGCCT	1 cut(s)	898
SsiI	CCGC	4 cut(s)	5, 91, 699, 873
SspMI	CTAG	2 cut(s)	245, 315
StuI	AGGCCT	1 cut(s)	898
StyI	CCWWGG	2 cut(s)	346, 403
TaaI	ACNGT	1 cut(s)	529
TaiI	ACGT	1 cut(s)	183
TaqI	TCGA	4 cut(s)	17, 120, 153, 204
TasI	AATT	1 cut(s)	354
TatI	WGTACW	1 cut(s)	579
TauI	GCSGC	1 cut(s)	7
Tru1I	TTAA	3 cut(s)	501, 858, 913
Tru9I	TTAA	3 cut(s)	501, 858, 913
TseFI	GTSAC	1 cut(s)	424
TseI	GCWGC	8 cut(s)	129, 165, 248, 304, 472, 491, 648, 765
Tsp45I	GTSAC	1 cut(s)	424
TspDTI	ATGAA	5 cut(s)	139, 450, 473, 684, 724
XceI	RCATGY	1 cut(s)	707
XhoI	CTCGAG	1 cut(s)	203
XmnI	GAANNNNTTC	2 cut(s)	146, 198
XspI	CTAG	2 cut(s)	245, 315
Zsp2I	ATGCAT	1 cut(s)	200

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.