pycom05g30230

Nitric oxide synthase-interacting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013491
Physical Location & Seq
Reverse (-)
30400299 .. 30402529
2231 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g30230.1

Sequence Viewer

Length: 912 bp
ATGCCGCAGAGGCACTCGAAGAACAACAACGACCTCGCTTTCTTCACCTACGACGAGAAGAAGAAGCTCGGCTATGGAACCCAGAGGGAGCGGCTCGGCAGGGACTCCATCAAGCCCTTCGACGCCTGCTGCCTCTGCCTCAAGCCCTTCATCGACCCCATGTGCTGCCAGAAAGGCCACGTCTTTTGCAAGGAATGCATTCTCGAGTGCTTGCTTTCTCAGAAGAAGGACATCCACAGAAAGCTAGCTGCACATACTGCTCAGCAGAAGCAAGAAAAGAACGAGGAAGATGATCGGTTAATGCAGCAGAAAGCTAGAGAGCTTGAGGCTTTTGATCAGCAAAACCACGGTGCAATTCCACAATACAATGATAGAAACCGGAGCCAAGATAAGACTGGTTTCCACGGGGCAAACAGTGTGAAGGTCACTTCTTATGAAGAAGAGGCTCTTCGGACAATGAAAGCCTTTTGGCTGCCTTCTGCTACACCGGCAGCTCCTGTTAAAGTAGATGCTCCCTCTATGAGCACAGTCTGTCCAGAAGGAAATGAGAAACTAAAGCTGAAGTCCCTATTCTCTGTGTATTACACAGAAGACACTAACGAGAAGAAAACTTCATCTCTGGATAAGACATTTATCTGTCCGAGCTGCAAGGTTACCTTGACCAACACGCTGTCACTCGTGGCCCTTAGTTCATGCGGGCATGTGTTTTGTAAGAAATGTGCTGATAAGTTCATGGCTGTAGATAAGGTTTGCCTTGTTTGCAGCAAGGGATGCAAAGAAAGGAACTTGGTGAACTTGGCAAAAGGGGGAACAGGCTTTGCAGGGCACGATGATAATCTTGAAGCAAGAGACTTCAAGCATTTGGGGAGTGGTTCCGGATTAGGGTTGGTGAGACCCGCCGCGAAGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rtf2 zf-NOSIP zf-RING_UBOX

Protein Analysis

304

Amino Acids

33.8

Weight (kDa)

8.8

Isoelectric Point (pI)

36.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-NOSIP PF15906 5 - 75 2.2e-15 Zinc-finger of nitric oxide synthase-interacting protein
Rtf2 PF04641 187 - 268 6.9e-08 Rtf2 RING-finger
zf-RING_5 PF14634 213 - 256 2.5e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013491)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61620
fragaria_vesca FvH4_3g03660
malus_domestica MD05G1329000.v1.1 MD10G1305900.v1.1
prunus_persica Prupe.4G033800_v2.0.a1
pyrus_communis pycom05g30230 pycom10g25820
rosa_chinensis RchiOBHm_Chr5g0005701
rosa_laevigata RLG00000031319
rosa_multiflora Rmu_sc0010248.1_g000004
rosa_roxburghii Rroxscaffold_1G00070140
rosa_rugosa Rorug04G0417400
rosa_samantha Rh5AG045000 Rh5BG043700 Rh5CG052500 Rh5DG043500
rosa_wichuraiana Rw0G008370 Rw5G004520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 91
AccII CGCG 1 cut(s) 902
AccIII TCCGGA 1 cut(s) 875
AciI CCGC 5 cut(s) 5, 91, 696, 897, 900
AcuI CTGAAG 1 cut(s) 581
AcyI GRCGYC 1 cut(s) 123
AfiI CCNNNNNNNGG 1 cut(s) 882
AgsI TTSAA 2 cut(s) 842, 856
AjiI CACGTC 1 cut(s) 181
AluBI AGCT 8 cut(s) 67, 244, 248, 314, 322, 494, 559, 645
AluI AGCT 8 cut(s) 67, 244, 248, 314, 322, 494, 559, 645
Alw21I GWGCWC 1 cut(s) 527
Alw26I GTCTC 2 cut(s) 843, 886
AlwNI CAGNNNCTG 1 cut(s) 497
Ama87I CYCGRG 1 cut(s) 203
Aor13HI TCCGGA 1 cut(s) 875
AoxI GGCC 2 cut(s) 175, 681
ApeKI GCWGC 8 cut(s) 129, 165, 248, 304, 472, 491, 645, 762
Asp700I GAANNNNTTC 1 cut(s) 198
AspS9I GGNCC 1 cut(s) 682
AsuHPI GGTGA 3 cut(s) 37, 802, 901
AsuNHI GCTAGC 1 cut(s) 244
AvaI CYCGRG 1 cut(s) 203
BaeGI GKGCMC 1 cut(s) 828
BauI CACGAG 1 cut(s) 677
BbsI GAAGAC 1 cut(s) 597
Bbv12I GWGCWC 1 cut(s) 527
BbvI GCAGC 8 cut(s) 116, 152, 235, 316, 459, 503, 632, 774
BccI CCATC 1 cut(s) 116
BclI TGATCA 1 cut(s) 334
BcoDI GTCTC 2 cut(s) 843, 886
BfaI CTAG 2 cut(s) 245, 315
BfmI CTRYAG 1 cut(s) 738
BglI GCCNNNNNGGC 2 cut(s) 10, 174
BlpI GCTNAGC 1 cut(s) 261
BmeT110I CYCGRG 1 cut(s) 203
BmgBI CACGTC 1 cut(s) 181
BmgT120I GGNCC 1 cut(s) 682
BmiI GGNNCC 3 cut(s) 79, 383, 874
BmsI GCATC 2 cut(s) 499, 761
BmtI GCTAGC 1 cut(s) 248
BpiI GAAGAC 1 cut(s) 597
Bpu1102I GCTNAGC 1 cut(s) 261
BpuEI CTTGAG 2 cut(s) 125, 344
BsaBI GATNNNNATC 1 cut(s) 834
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 886
BsaJI CCNNGG 2 cut(s) 346, 403
BsaWI WCCGGW 2 cut(s) 378, 875
Bsc4I CCNNNNNNNGG 1 cut(s) 882
Bse118I RCCGGY 1 cut(s) 487
Bse1I ACTGG 1 cut(s) 400
Bse8I GATNNNNATC 1 cut(s) 834
BseAI TCCGGA 1 cut(s) 875
BseDI CCNNGG 2 cut(s) 346, 403
BseGI GGATG 2 cut(s) 231, 776
BseJI GATNNNNATC 1 cut(s) 834
BseLI CCNNNNNNNGG 1 cut(s) 882
BseMII CTCAG 2 cut(s) 233, 275
BseNI ACTGG 1 cut(s) 400
BseSI GKGCMC 1 cut(s) 828
BseXI GCAGC 8 cut(s) 116, 152, 235, 316, 459, 503, 632, 774
BsgI GTGCAG 1 cut(s) 234
Bsh1236I CGCG 1 cut(s) 902
BshFI GGCC 2 cut(s) 177, 683
BsiHKAI GWGCWC 1 cut(s) 527
BsiHKCI CYCGRG 1 cut(s) 203
BsiSI CCGG 3 cut(s) 379, 488, 876
BslFI GGGAC 2 cut(s) 116, 550
BslI CCNNNNNNNGG 1 cut(s) 882
BsmAI GTCTC 2 cut(s) 843, 886
BsmFI GGGAC 2 cut(s) 116, 550
BsmI GAATGC 2 cut(s) 198, 200
BsnI GGCC 2 cut(s) 177, 683
Bso31I GGTCTC 1 cut(s) 886
BsoBI CYCGRG 1 cut(s) 203
Bsp1286I GDGCHC 2 cut(s) 527, 828
Bsp13I TCCGGA 1 cut(s) 875
Bsp143I GATC 2 cut(s) 292, 334
Bsp1720I GCTNAGC 1 cut(s) 261
BspACI CCGC 5 cut(s) 5, 91, 696, 897, 900
BspANI GGCC 2 cut(s) 177, 683
BspCNI CTCAG 2 cut(s) 232, 274
BspEI TCCGGA 1 cut(s) 875
BspFNI CGCG 1 cut(s) 902
BspLI GGNNCC 3 cut(s) 79, 383, 874
BspOI GCTAGC 1 cut(s) 248
BspQI GCTCTTC 1 cut(s) 453
BspTNI GGTCTC 1 cut(s) 886
BsrBI CCGCTC 1 cut(s) 91
BsrFI RCCGGY 1 cut(s) 487
BsrI ACTGG 1 cut(s) 400
BssAI RCCGGY 1 cut(s) 487
BssECI CCNNGG 2 cut(s) 346, 403
BssMI GATC 2 cut(s) 292, 334
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 1 cut(s) 677
Bst2BI CACGAG 1 cut(s) 677
Bst4CI ACNGT 3 cut(s) 350, 416, 529
Bst6I CTCTTC 2 cut(s) 435, 453
BstACI GRCGYC 1 cut(s) 123
BstAPI GCANNNNNTGC 3 cut(s) 195, 257, 771
BstC8I GCNNGC 4 cut(s) 127, 212, 246, 698
BstDEI CTNAG 3 cut(s) 219, 261, 686
BstDSI CCRYGG 2 cut(s) 346, 403
BstEII GGTNACC 1 cut(s) 652
BstF5I GGATG 2 cut(s) 231, 776
BstFNI CGCG 1 cut(s) 902
BstKTI GATC 2 cut(s) 295, 337
BstMAI GTCTC 2 cut(s) 843, 886
BstMBI GATC 2 cut(s) 292, 334
BstMWI GCNNNNNNNGC 8 cut(s) 10, 135, 174, 195, 257, 488, 759, 771
BstNSI RCATGY 1 cut(s) 704
BstPI GGTNACC 1 cut(s) 652
BstSFI CTRYAG 1 cut(s) 738
BstSLI GKGCMC 1 cut(s) 828
BstUI CGCG 1 cut(s) 902
BstV1I GCAGC 8 cut(s) 116, 152, 235, 316, 459, 503, 632, 774
BstV2I GAAGAC 1 cut(s) 597
BsuRI GGCC 2 cut(s) 177, 683
BtgI CCRYGG 2 cut(s) 346, 403
BtrI CACGTC 1 cut(s) 181
BtsCI GGATG 2 cut(s) 231, 776
BtsIMutI CAGTG 1 cut(s) 421
Cac8I GCNNGC 4 cut(s) 127, 212, 246, 698
CaiI CAGNNNCTG 1 cut(s) 497
Cfr10I RCCGGY 1 cut(s) 487
Cfr13I GGNCC 1 cut(s) 682
CseI GACGC 1 cut(s) 131
CspCI CAANNNNNGTGG 2 cut(s) 167, 202
CviAII CATG 4 cut(s) 160, 693, 701, 733
DdeI CTNAG 3 cut(s) 219, 261, 686
DpnI GATC 2 cut(s) 294, 336
DpnII GATC 2 cut(s) 292, 334
Eam1104I CTCTTC 2 cut(s) 435, 453
EarI CTCTTC 2 cut(s) 435, 453
Eco31I GGTCTC 1 cut(s) 886
Eco57I CTGAAG 1 cut(s) 581
Eco88I CYCGRG 1 cut(s) 203
Eco91I GGTNACC 1 cut(s) 652
EcoO65I GGTNACC 1 cut(s) 652
EcoT22I ATGCAT 1 cut(s) 200
FaeI CATG 4 cut(s) 163, 696, 704, 736
FaiI YATR 8 cut(s) 75, 161, 255, 435, 521, 694, 702, 734
FalI AAGNNNNNCTT 4 cut(s) 432, 464, 641, 673
FaqI GGGAC 2 cut(s) 116, 550
FatI CATG 4 cut(s) 159, 692, 700, 732
FauI CCCGC 2 cut(s) 689, 904
FbaI TGATCA 1 cut(s) 334
FokI GGATG 2 cut(s) 218, 783
FspBI CTAG 2 cut(s) 245, 315
HaeIII GGCC 2 cut(s) 177, 683
HapII CCGG 3 cut(s) 379, 488, 876
HgaI GACGC 1 cut(s) 131
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 4 cut(s) 163, 696, 704, 736
HinfI GANTC 1 cut(s) 104
HpaII CCGG 3 cut(s) 379, 488, 876
HphI GGTGA 3 cut(s) 37, 802, 901
Hpy166II GTNNAC 1 cut(s) 793
Hpy188I TCNGA 3 cut(s) 222, 453, 642
Hpy188III TCNNGA 5 cut(s) 203, 536, 620, 839, 876
Hpy8I GTNNAC 1 cut(s) 793
Hpy99I CGWCG 2 cut(s) 56, 125
HpyAV CCTTC 6 cut(s) 127, 157, 220, 415, 486, 533
HpyCH4III ACNGT 3 cut(s) 350, 416, 529
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 9 cut(s) 189, 198, 251, 304, 353, 648, 762, 774, 821
HpyF10VI GCNNNNNNNGC 8 cut(s) 10, 135, 174, 195, 257, 488, 759, 771
HpyF3I CTNAG 3 cut(s) 219, 261, 686
HpySE526I ACGT 1 cut(s) 180
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 4 cut(s) 163, 696, 704, 736
Kpn2I TCCGGA 1 cut(s) 875
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 2 cut(s) 292, 334
LguI GCTCTTC 1 cut(s) 453
LmnI GCTCC 4 cut(s) 88, 381, 499, 517
Lsp1109I GCAGC 8 cut(s) 116, 152, 235, 316, 459, 503, 632, 774
LweI GCATC 2 cut(s) 499, 761
MaeI CTAG 2 cut(s) 245, 315
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 3 cut(s) 424, 652, 672
MalI GATC 2 cut(s) 294, 336
MbiI CCGCTC 1 cut(s) 91
MboI GATC 2 cut(s) 292, 334
MhlI GDGCHC 2 cut(s) 527, 828
MluCI AATT 1 cut(s) 354
MlyI GAGTC 1 cut(s) 98
MnlI CCTC 9 cut(s) 3, 44, 78, 143, 149, 277, 319, 436, 526
Mph1103I ATGCAT 1 cut(s) 200
MroI TCCGGA 1 cut(s) 875
MroXI GAANNNNTTC 1 cut(s) 198
MseI TTAA 3 cut(s) 299, 501, 910
MspI CCGG 3 cut(s) 379, 488, 876
Mva1269I GAATGC 2 cut(s) 198, 200
MvnI CGCG 1 cut(s) 902
MwoI GCNNNNNNNGC 8 cut(s) 10, 135, 174, 195, 257, 488, 759, 771
NdeII GATC 2 cut(s) 292, 334
NheI GCTAGC 1 cut(s) 244
NlaIII CATG 4 cut(s) 163, 696, 704, 736
NlaIV GGNNCC 3 cut(s) 79, 383, 874
NmeAIII GCCGAG 2 cut(s) 48, 75
NmuCI GTSAC 2 cut(s) 424, 672
NsiI ATGCAT 1 cut(s) 200
NspI RCATGY 1 cut(s) 704
PaeR7I CTCGAG 1 cut(s) 203
PciSI GCTCTTC 1 cut(s) 453
PctI GAATGC 2 cut(s) 198, 200
PdmI GAANNNNTTC 1 cut(s) 198
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
PspEI GGTNACC 1 cut(s) 652
PspN4I GGNNCC 3 cut(s) 79, 383, 874
PspPI GGNCC 1 cut(s) 682
PstNI CAGNNNCTG 1 cut(s) 497
SapI GCTCTTC 1 cut(s) 453
SaqAI TTAA 3 cut(s) 299, 501, 910
Sau3AI GATC 2 cut(s) 292, 334
Sau96I GGNCC 1 cut(s) 682
SchI GAGTC 1 cut(s) 98
SduI GDGCHC 2 cut(s) 527, 828
SfaNI GCATC 2 cut(s) 499, 761
SfcI CTRYAG 1 cut(s) 738
Sfr274I CTCGAG 1 cut(s) 203
SlaI CTCGAG 1 cut(s) 203
SmlI CTYRAG 3 cut(s) 140, 203, 323
SmoI CTYRAG 3 cut(s) 140, 203, 323
Sse9I AATT 1 cut(s) 354
SsiI CCGC 5 cut(s) 5, 91, 696, 897, 900
SspMI CTAG 2 cut(s) 245, 315
TaaI ACNGT 3 cut(s) 350, 416, 529
TaiI ACGT 1 cut(s) 183
TaqI TCGA 4 cut(s) 17, 120, 153, 204
TasI AATT 1 cut(s) 354
TauI GCSGC 3 cut(s) 7, 94, 902
Tru1I TTAA 3 cut(s) 299, 501, 910
Tru9I TTAA 3 cut(s) 299, 501, 910
TscAI CASTG 1 cut(s) 421
TseFI GTSAC 2 cut(s) 424, 672
TseI GCWGC 8 cut(s) 129, 165, 248, 304, 472, 491, 645, 762
Tsp45I GTSAC 2 cut(s) 424, 672
TspDTI ATGAA 6 cut(s) 139, 450, 473, 603, 681, 721
TspRI CASTG 1 cut(s) 421
XceI RCATGY 1 cut(s) 704
XcmI CCANNNNNNNNNTGG 1 cut(s) 392
XhoI CTCGAG 1 cut(s) 203
XmnI GAANNNNTTC 1 cut(s) 198
XspI CTAG 2 cut(s) 245, 315
Zsp2I ATGCAT 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.