Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0013491

Physical Location & Seq

Genomic Coordinates Forward (+)

3767916 .. 3771136

Gene Structure 3221 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG045000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1101 bp

ATGAAACGACATGTGGTTTTTGTTGCGGTTCTAATTCCCAATCGAACCGAACCCGGATGTGGCCCAATACATATTTCGGGGCAGCTGCTTCTGCAGTCTGCATCTCTCTCTCCCTCTTGGTCAATCCCCAAATCAAAGGACTACCCCCAAATCCCTTACTCTTCTTCTTCTCTCAGAGATAATAATATGCCTCAGAGACACTCGAAGAACAACAACGACCTCGCGTTCTTCACATACGACGAGAAGCGAAAGCTTGGGTATGGAACCCAGAGGGAGAGACTCGGCAGAGACTCCATCAAACCCTTCGATGCTTGTTGCCTCTGCTTGAAGCCCTTCATCGACCCCATGAGTTGCCAGAAAGGCCATGTCTTTTGCAAGGAGTGCATTCTCGAATGCTTGCTTTCCCAGAAGAAAGACATCCACAGAAAGCAAGTTGCACATAATAGTCAGCAGAAGCAAGACAAGGAAGATGAAGAAGAGAAGTTAAGGCAACAGAAAGCCAGAGAGCTTGAGGCTTTTGATCAGCAAAACCATGGTGCAGTACCGCACTACAATGATAGAAACCAGGACCGGGATAAGAATGGTTTTCATGGGGCAAATAGTGTGAAGGTCACTTCTTACGAAGAAGAGGCCCTTCGGACAATGAAGGCATTTTGGCTGCCTTCTGCTACGCCAGAAGCTCCTGTTAAAGTGGCTGCCCCCCCTGAATGCACAACCTGTCCAGAAGGAAGCGAGAAACTAAAGCTGAAGTCTCTTTTCCCTATCCATTTCACTGAAGACACCAACGACGAGAAGAAATCAACGCCTCTTGACAAGACATTTATCTGTCCGAGCTGCAAGGTTACCTTAACAAACACGCTATCACTCGTGGCTCTTAGCTCATGTGGCCACGTCTTCTGTAAGAAGTGTGCTGATAAGTTCATACCTGTAGATAAGGTTTGTCTCGTTTGCAACAAGGGATGCAAAGAAAGGAACTTGGTGAACCTGAAAAAAGGAGGGACCGGCTTTGCTGGCCACGACGATGATCTTGAAGCCAAAGATTTCAAGCACTTGGGGAGTGGCTCGGGTCTTGGGTTAGTGAGACCCGCAGCAAAGACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009791 GO:0009987 GO:0016567 GO:0016604 GO:0016607 GO:0016740 GO:0019538 GO:0019787 GO:0031647 GO:0031648 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0048856 GO:0050896 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070013 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Rtf2 zf-NOSIP zf-RING_UBOX

Protein Analysis

366

Amino Acids

40.92

Weight (kDa)

8.76

Isoelectric Point (pI)

51.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-NOSIP	PF15906	67 - 137	8.7e-16	Zinc-finger of nitric oxide synthase-interacting protein
Rtf2	PF04641	248 - 330	1e-08	Rtf2 RING-finger
zf-RING_5	PF14634	276 - 319	7.4e-06	zinc-RING finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013491)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G61620
fragaria_vesca	FvH4_3g03660
malus_domestica	MD05G1329000.v1.1 MD10G1305900.v1.1
prunus_persica	Prupe.4G033800_v2.0.a1
pyrus_communis	pycom05g30230 pycom10g25820
rosa_chinensis	RchiOBHm_Chr5g0005701
rosa_laevigata	RLG00000031319
rosa_multiflora	Rmu_sc0010248.1_g000004
rosa_roxburghii	Rroxscaffold_1G00070140
rosa_rugosa	Rorug04G0417400
rosa_samantha	Rh5AG045000 Rh5BG043700 Rh5CG052500 Rh5DG043500
rosa_wichuraiana	Rw0G008370 Rw5G004520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	224
AciI	CCGC	3 cut(s)	26, 545, 1086
AcoI	YGGCCR	2 cut(s)	886, 1012
AcuI	CTGAAG	2 cut(s)	767, 795
AfaI	GTAC	1 cut(s)	543
AfiI	CCNNNNNNNGG	2 cut(s)	59, 571
AflIII	ACRYGT	1 cut(s)	10
AgsI	TTSAA	3 cut(s)	328, 1031, 1045
AjiI	CACGTC	1 cut(s)	892
AjnI	CCWGG	1 cut(s)	564
AjuI	GAANNNNNNNTTGG	2 cut(s)	58, 90
AluBI	AGCT	7 cut(s)	85, 253, 508, 680, 745, 834, 879
AluI	AGCT	7 cut(s)	85, 253, 508, 680, 745, 834, 879
Alw26I	GTCTC	6 cut(s)	190, 271, 282, 756, 947, 1075
Ama87I	CYCGRG	1 cut(s)	1063
AoxI	GGCC	5 cut(s)	61, 361, 630, 886, 1012
ApeKI	GCWGC	6 cut(s)	82, 85, 658, 695, 834, 1088
Asp700I	GAANNNNTTC	1 cut(s)	332
AspS9I	GGNCC	4 cut(s)	62, 568, 631, 999
AsuC2I	CCSGG	2 cut(s)	54, 572
AsuHPI	GGTGA	1 cut(s)	991
AvaI	CYCGRG	1 cut(s)	1063
AvaII	GGWCC	2 cut(s)	568, 999
BalI	TGGCCA	2 cut(s)	888, 1014
BauI	CACGAG	1 cut(s)	866
BbsI	GAAGAC	2 cut(s)	783, 886
BbvI	GCAGC	5 cut(s)	72, 94, 645, 682, 821
BccI	CCATC	1 cut(s)	302
BciT130I	CCWGG	1 cut(s)	566
BclI	TGATCA	1 cut(s)	520
BcnI	CCSGG	2 cut(s)	54, 572
BcoDI	GTCTC	6 cut(s)	190, 271, 282, 756, 947, 1075
BfmI	CTRYAG	2 cut(s)	92, 927
BglI	GCCNNNNNGGC	1 cut(s)	360
BisI	GCNGC	6 cut(s)	83, 86, 659, 696, 835, 1089
BlsI	GCNGC	6 cut(s)	84, 87, 660, 697, 836, 1090
Bme1390I	CCNGG	3 cut(s)	54, 566, 572
Bme18I	GGWCC	2 cut(s)	568, 999
BmeT110I	CYCGRG	1 cut(s)	1063
BmgBI	CACGTC	1 cut(s)	892
BmgT120I	GGNCC	4 cut(s)	62, 568, 631, 999
BmiI	GGNNCC	2 cut(s)	265, 1000
BmrFI	CCNGG	3 cut(s)	54, 566, 572
BmsI	GCATC	3 cut(s)	110, 298, 950
BpiI	GAAGAC	2 cut(s)	783, 886
BpuEI	CTTGAG	1 cut(s)	530
BpuMI	CCSGG	2 cut(s)	54, 572
BsaI	GGTCTC	1 cut(s)	1075
BsaJI	CCNNGG	1 cut(s)	532
Bsc4I	CCNNNNNNNGG	2 cut(s)	59, 571
Bse118I	RCCGGY	1 cut(s)	1001
BseBI	CCWGG	1 cut(s)	566
BseDI	CCNNGG	1 cut(s)	532
BseGI	GGATG	3 cut(s)	62, 417, 965
BseLI	CCNNNNNNNGG	2 cut(s)	59, 571
BseMII	CTCAG	2 cut(s)	187, 206
BseXI	GCAGC	5 cut(s)	72, 94, 645, 682, 821
BsgI	GTGCAG	1 cut(s)	558
Bsh1236I	CGCG	1 cut(s)	224
BshFI	GGCC	5 cut(s)	63, 363, 632, 888, 1014
BsiHKCI	CYCGRG	1 cut(s)	1063
BsiSI	CCGG	3 cut(s)	54, 571, 1002
BslFI	GGGAC	1 cut(s)	1012
BslI	CCNNNNNNNGG	2 cut(s)	59, 571
BsmAI	GTCTC	6 cut(s)	190, 271, 282, 756, 947, 1075
BsmFI	GGGAC	1 cut(s)	1012
BsmI	GAATGC	3 cut(s)	384, 398, 713
BsnI	GGCC	5 cut(s)	63, 363, 632, 888, 1014
Bso31I	GGTCTC	1 cut(s)	1075
BsoBI	CYCGRG	1 cut(s)	1063
Bsp143I	GATC	2 cut(s)	520, 1024
Bsp19I	CCATGG	1 cut(s)	532
BspACI	CCGC	3 cut(s)	26, 545, 1086
BspANI	GGCC	5 cut(s)	63, 363, 632, 888, 1014
BspCNI	CTCAG	2 cut(s)	186, 205
BspFNI	CGCG	1 cut(s)	224
BspLI	GGNNCC	2 cut(s)	265, 1000
BspMAI	CTGCAG	1 cut(s)	96
BspTNI	GGTCTC	1 cut(s)	1075
BsrFI	RCCGGY	1 cut(s)	1001
BssAI	RCCGGY	1 cut(s)	1001
BssECI	CCNNGG	1 cut(s)	532
BssMI	GATC	2 cut(s)	520, 1024
BssSI	CACGAG	1 cut(s)	866
BssT1I	CCWWGG	1 cut(s)	532
Bst2BI	CACGAG	1 cut(s)	866
Bst2UI	CCWGG	1 cut(s)	566
Bst6I	CTCTTC	3 cut(s)	166, 471, 621
BstAPI	GCANNNNNTGC	1 cut(s)	381
BstC8I	GCNNGC	2 cut(s)	398, 1012
BstDEI	CTNAG	3 cut(s)	173, 192, 875
BstDSI	CCRYGG	1 cut(s)	532
BstEII	GGTNACC	1 cut(s)	841
BstF5I	GGATG	3 cut(s)	62, 417, 965
BstFNI	CGCG	1 cut(s)	224
BstKTI	GATC	2 cut(s)	523, 1027
BstMAI	GTCTC	6 cut(s)	190, 271, 282, 756, 947, 1075
BstMBI	GATC	2 cut(s)	520, 1024
BstMWI	GCNNNNNNNGC	5 cut(s)	91, 360, 381, 885, 1011
BstNI	CCWGG	1 cut(s)	566
BstNSI	RCATGY	1 cut(s)	14
BstPI	GGTNACC	1 cut(s)	841
BstSCI	CCNGG	3 cut(s)	52, 564, 570
BstSFI	CTRYAG	2 cut(s)	92, 927
BstUI	CGCG	1 cut(s)	224
BstV1I	GCAGC	5 cut(s)	72, 94, 645, 682, 821
BstV2I	GAAGAC	2 cut(s)	783, 886
BsuRI	GGCC	5 cut(s)	63, 363, 632, 888, 1014
BtgI	CCRYGG	1 cut(s)	532
BtrI	CACGTC	1 cut(s)	892
BtsCI	GGATG	3 cut(s)	62, 417, 965
BtsIMutI	CAGTG	1 cut(s)	771
Cac8I	GCNNGC	2 cut(s)	398, 1012
Cfr10I	RCCGGY	1 cut(s)	1001
Cfr13I	GGNCC	4 cut(s)	62, 568, 631, 999
Csp6I	GTAC	1 cut(s)	542
CviAII	CATG	6 cut(s)	11, 346, 365, 533, 590, 882
CviQI	GTAC	1 cut(s)	542
DdeI	CTNAG	3 cut(s)	173, 192, 875
DpnI	GATC	2 cut(s)	522, 1026
DpnII	GATC	2 cut(s)	520, 1024
EaeI	YGGCCR	2 cut(s)	886, 1012
Eam1104I	CTCTTC	3 cut(s)	166, 471, 621
EarI	CTCTTC	3 cut(s)	166, 471, 621
Eco130I	CCWWGG	1 cut(s)	532
Eco31I	GGTCTC	1 cut(s)	1075
Eco47I	GGWCC	2 cut(s)	568, 999
Eco57I	CTGAAG	2 cut(s)	767, 795
Eco88I	CYCGRG	1 cut(s)	1063
Eco91I	GGTNACC	1 cut(s)	841
EcoO109I	RGGNCCY	1 cut(s)	631
EcoO65I	GGTNACC	1 cut(s)	841
EcoRII	CCWGG	1 cut(s)	564
EcoT14I	CCWWGG	1 cut(s)	532
ErhI	CCWWGG	1 cut(s)	532
FaeI	CATG	6 cut(s)	14, 349, 368, 536, 593, 885
FalI	AAGNNNNNCTT	4 cut(s)	618, 650, 830, 862
FaqI	GGGAC	1 cut(s)	1012
FatI	CATG	6 cut(s)	10, 345, 364, 532, 589, 881
FauI	CCCGC	1 cut(s)	1093
FbaI	TGATCA	1 cut(s)	520
Fnu4HI	GCNGC	6 cut(s)	83, 86, 659, 696, 835, 1089
FokI	GGATG	3 cut(s)	69, 404, 972
Fsp4HI	GCNGC	6 cut(s)	83, 86, 659, 696, 835, 1089
GluI	GCNGC	6 cut(s)	83, 86, 659, 696, 835, 1089
HaeIII	GGCC	5 cut(s)	63, 363, 632, 888, 1014
HapII	CCGG	3 cut(s)	54, 571, 1002
Hin1II	CATG	6 cut(s)	14, 349, 368, 536, 593, 885
HindIII	AAGCTT	1 cut(s)	251
HinfI	GANTC	2 cut(s)	279, 290
HpaII	CCGG	3 cut(s)	54, 571, 1002
HphI	GGTGA	1 cut(s)	991
Hpy166II	GTNNAC	1 cut(s)	982
Hpy188I	TCNGA	4 cut(s)	176, 195, 639, 831
Hpy188III	TCNNGA	4 cut(s)	389, 722, 809, 1028
Hpy8I	GTNNAC	1 cut(s)	982
Hpy99I	CGWCG	3 cut(s)	242, 791, 1022
HpyAV	CCTTC	7 cut(s)	313, 343, 601, 640, 644, 672, 719
HpyCH4IV	ACGT	1 cut(s)	891
HpyF10VI	GCNNNNNNNGC	5 cut(s)	91, 360, 381, 885, 1011
HpyF3I	CTNAG	3 cut(s)	173, 192, 875
HpySE526I	ACGT	1 cut(s)	891
Hsp92II	CATG	6 cut(s)	14, 349, 368, 536, 593, 885
Ksp22I	TGATCA	1 cut(s)	520
Kzo9I	GATC	2 cut(s)	520, 1024
LmnI	GCTCC	1 cut(s)	685
Lsp1109I	GCAGC	5 cut(s)	72, 94, 645, 682, 821
LweI	GCATC	3 cut(s)	110, 298, 950
MaeII	ACGT	1 cut(s)	891
MaeIII	GTNAC	2 cut(s)	610, 841
MalI	GATC	2 cut(s)	522, 1026
MboI	GATC	2 cut(s)	520, 1024
MlsI	TGGCCA	2 cut(s)	888, 1014
MluCI	AATT	1 cut(s)	33
MluNI	TGGCCA	2 cut(s)	888, 1014
MlyI	GAGTC	2 cut(s)	273, 284
MnlI	CCTC	9 cut(s)	124, 201, 230, 264, 329, 505, 622, 816, 989
Mox20I	TGGCCA	2 cut(s)	888, 1014
MroXI	GAANNNNTTC	1 cut(s)	332
MscI	TGGCCA	2 cut(s)	888, 1014
MseI	TTAA	4 cut(s)	485, 687, 848, 1099
MslI	CAYNNNNRTG	2 cut(s)	552, 1020
Msp20I	TGGCCA	2 cut(s)	888, 1014
MspA1I	CMGCKG	1 cut(s)	85
MspI	CCGG	3 cut(s)	54, 571, 1002
MspR9I	CCNGG	3 cut(s)	54, 566, 572
Mva1269I	GAATGC	3 cut(s)	384, 398, 713
MvaI	CCWGG	1 cut(s)	566
MvnI	CGCG	1 cut(s)	224
MwoI	GCNNNNNNNGC	5 cut(s)	91, 360, 381, 885, 1011
NciI	CCSGG	2 cut(s)	54, 572
NcoI	CCATGG	1 cut(s)	532
NdeII	GATC	2 cut(s)	520, 1024
NlaIII	CATG	6 cut(s)	14, 349, 368, 536, 593, 885
NlaIV	GGNNCC	2 cut(s)	265, 1000
NmeAIII	GCCGAG	1 cut(s)	261
NmuCI	GTSAC	1 cut(s)	610
NspI	RCATGY	1 cut(s)	14
PciI	ACATGT	1 cut(s)	10
PctI	GAATGC	3 cut(s)	384, 398, 713
PdmI	GAANNNNTTC	1 cut(s)	332
PkrI	GCNGC	6 cut(s)	84, 87, 660, 697, 836, 1090
PleI	GAGTC	2 cut(s)	273, 284
PpsI	GAGTC	2 cut(s)	273, 284
PscI	ACATGT	1 cut(s)	10
Psp6I	CCWGG	1 cut(s)	564
PspEI	GGTNACC	1 cut(s)	841
PspGI	CCWGG	1 cut(s)	564
PspN4I	GGNNCC	2 cut(s)	265, 1000
PspPI	GGNCC	4 cut(s)	62, 568, 631, 999
PstI	CTGCAG	1 cut(s)	96
PvuII	CAGCTG	1 cut(s)	85
RsaI	GTAC	1 cut(s)	543
RsaNI	GTAC	1 cut(s)	542
RseI	CAYNNNNRTG	2 cut(s)	552, 1020
SaqAI	TTAA	4 cut(s)	485, 687, 848, 1099
SatI	GCNGC	6 cut(s)	83, 86, 659, 696, 835, 1089
Sau3AI	GATC	2 cut(s)	520, 1024
Sau96I	GGNCC	4 cut(s)	62, 568, 631, 999
SchI	GAGTC	2 cut(s)	273, 284
ScrFI	CCNGG	3 cut(s)	54, 566, 572
SfaNI	GCATC	3 cut(s)	110, 298, 950
SfcI	CTRYAG	2 cut(s)	92, 927
SinI	GGWCC	2 cut(s)	568, 999
SmiMI	CAYNNNNRTG	2 cut(s)	552, 1020
SmlI	CTYRAG	1 cut(s)	509
SmoI	CTYRAG	1 cut(s)	509
Sse9I	AATT	1 cut(s)	33
SsiI	CCGC	3 cut(s)	26, 545, 1086
StyD4I	CCNGG	3 cut(s)	52, 564, 570
StyI	CCWWGG	1 cut(s)	532
TaiI	ACGT	1 cut(s)	894
TaqI	TCGA	5 cut(s)	43, 203, 306, 339, 390
TasI	AATT	1 cut(s)	33
Tru1I	TTAA	4 cut(s)	485, 687, 848, 1099
Tru9I	TTAA	4 cut(s)	485, 687, 848, 1099
TscAI	CASTG	1 cut(s)	778
TseFI	GTSAC	1 cut(s)	610
TseI	GCWGC	6 cut(s)	82, 85, 658, 695, 834, 1088
Tsp45I	GTSAC	1 cut(s)	610
TspDTI	ATGAA	6 cut(s)	17, 325, 486, 578, 659, 910
TspRI	CASTG	1 cut(s)	778
VpaK11BI	GGWCC	2 cut(s)	568, 999
XceI	RCATGY	1 cut(s)	14
XmnI	GAANNNNTTC	1 cut(s)	332

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.