Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0013491

Physical Location & Seq

Genomic Coordinates Forward (+)

4745376 .. 4747798

Gene Structure 2423 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G004520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 861 bp

ATGCCTCAGAGACACTCGAAGAACAACAACGACCTCGCGTTCTTCACATACGACGAGAAGCGAAAGCTTGGGTATGGAACCCAGAGGGAGAGACTCGGCAGAGACTCCATCAAACCCTTCGATGCTTGTTGCCTCTGCTTGAAGCCCTTCATCGACCCCATGAGTTGCCAGAAAGGCCATGTCTTTTGCAAGGAGTGCATTCTCGAATGCTTGCTTTCCCAGAAGAAAGACATCCACAGAAAGCAAGTTGCACATAATAGTCAGCAGAAGCAAGACAAGGAAGATGAAGAAGAGAAGTTAAGGCAACAGAAAGCCAGAGAGCTTGAGGCTTTTGATCAGCAAAACCATGGTGCAGTACCGCACTACAATGATAGAAACCAGGACCGGGATAAGAATGGTTTTCATGGGGCAAATAGTGTGAAGGTCACTTCTTACGAAGAAGAGGCGCTTCGGACAATGAAGGCATTTTGGCTGCCTTCTGCTACGCCAGAAGCTAAACTAAAGCTGAAGTCTCTTTTCCCTATCCATTTCACTGAAGACACCAACGACGAGAAGAAATCAACGCCTCTTGACAAGACATTTATCTGTCCGAGCTGCAAGGTTACTTTAACAAACACGCTATCACTCGTGGCTCTTAGCTCATGTGGCCACGTCTTCTGTAAGAAGTGTGCTGATAAGTTCATACCTGTAGATAAGGTTTGTCTCGTTTGCAACAAGGGATGCAAAGAAAGGAACTTGGTGAACCTGAAAAAAGGAGGGACCGGCTTTGCTGGCCACGACGATGATCTTGAAGCCAAAGATTTCAAGCACTTGGGGAGTGGCTCAGGTCTTGGGTTAGTGAGACCCGCAGCAAAGACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009791 GO:0009987 GO:0016567 GO:0016604 GO:0016607 GO:0016740 GO:0019538 GO:0019787 GO:0031647 GO:0031648 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0048856 GO:0050896 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070013 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Rtf2 zf-NOSIP zf-RING_UBOX

Protein Analysis

286

Amino Acids

32.32

Weight (kDa)

8.79

Isoelectric Point (pI)

42.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-NOSIP	PF15906	5 - 75	5.7e-16	Zinc-finger of nitric oxide synthase-interacting protein
Rtf2	PF04641	168 - 250	9.6e-09	Rtf2 RING-finger
zf-RING_5	PF14634	196 - 239	5.1e-06	zinc-RING finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0013491)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G61620
fragaria_vesca	FvH4_3g03660
malus_domestica	MD05G1329000.v1.1 MD10G1305900.v1.1
prunus_persica	Prupe.4G033800_v2.0.a1
pyrus_communis	pycom05g30230 pycom10g25820
rosa_chinensis	RchiOBHm_Chr5g0005701
rosa_laevigata	RLG00000031319
rosa_multiflora	Rmu_sc0010248.1_g000004
rosa_roxburghii	Rroxscaffold_1G00070140
rosa_rugosa	Rorug04G0417400
rosa_samantha	Rh5AG045000 Rh5BG043700 Rh5CG052500 Rh5DG043500
rosa_wichuraiana	Rw0G008370 Rw5G004520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	38
AciI	CCGC	2 cut(s)	359, 846
AcoI	YGGCCR	2 cut(s)	646, 772
AcuI	CTGAAG	2 cut(s)	527, 555
AfaI	GTAC	1 cut(s)	357
AfiI	CCNNNNNNNGG	1 cut(s)	385
AgsI	TTSAA	3 cut(s)	142, 791, 805
AjiI	CACGTC	1 cut(s)	652
AjnI	CCWGG	1 cut(s)	378
AluBI	AGCT	6 cut(s)	67, 322, 494, 505, 594, 639
AluI	AGCT	6 cut(s)	67, 322, 494, 505, 594, 639
Alw26I	GTCTC	6 cut(s)	4, 85, 96, 516, 707, 835
AoxI	GGCC	3 cut(s)	175, 646, 772
ApeKI	GCWGC	3 cut(s)	472, 594, 848
Asp700I	GAANNNNTTC	1 cut(s)	146
AspLEI	GCGC	1 cut(s)	448
AspS9I	GGNCC	2 cut(s)	382, 759
AsuC2I	CCSGG	1 cut(s)	386
AsuHPI	GGTGA	1 cut(s)	751
AvaII	GGWCC	2 cut(s)	382, 759
BalI	TGGCCA	2 cut(s)	648, 774
BauI	CACGAG	1 cut(s)	626
BbsI	GAAGAC	2 cut(s)	543, 646
BbvI	GCAGC	2 cut(s)	459, 581
BccI	CCATC	1 cut(s)	116
BciT130I	CCWGG	1 cut(s)	380
BclI	TGATCA	1 cut(s)	334
BcnI	CCSGG	1 cut(s)	386
BcoDI	GTCTC	6 cut(s)	4, 85, 96, 516, 707, 835
BfmI	CTRYAG	1 cut(s)	687
BfoI	RGCGCY	1 cut(s)	449
BglI	GCCNNNNNGGC	1 cut(s)	174
BisI	GCNGC	3 cut(s)	473, 595, 849
BlsI	GCNGC	3 cut(s)	474, 596, 850
Bme1390I	CCNGG	2 cut(s)	380, 386
Bme18I	GGWCC	2 cut(s)	382, 759
BmgBI	CACGTC	1 cut(s)	652
BmgT120I	GGNCC	2 cut(s)	382, 759
BmiI	GGNNCC	2 cut(s)	79, 760
BmrFI	CCNGG	2 cut(s)	380, 386
BmsI	GCATC	2 cut(s)	112, 710
BpiI	GAAGAC	2 cut(s)	543, 646
Bpu10I	CCTNAGC	1 cut(s)	823
BpuEI	CTTGAG	1 cut(s)	344
BpuMI	CCSGG	1 cut(s)	386
BsaI	GGTCTC	1 cut(s)	835
BsaJI	CCNNGG	1 cut(s)	346
Bsc4I	CCNNNNNNNGG	1 cut(s)	385
Bse118I	RCCGGY	1 cut(s)	761
BseBI	CCWGG	1 cut(s)	380
BseDI	CCNNGG	1 cut(s)	346
BseGI	GGATG	2 cut(s)	231, 725
BseLI	CCNNNNNNNGG	1 cut(s)	385
BseMII	CTCAG	2 cut(s)	20, 837
BseXI	GCAGC	2 cut(s)	459, 581
BsgI	GTGCAG	1 cut(s)	372
Bsh1236I	CGCG	1 cut(s)	38
BshFI	GGCC	3 cut(s)	177, 648, 774
BsiSI	CCGG	2 cut(s)	385, 762
BslFI	GGGAC	1 cut(s)	772
BslI	CCNNNNNNNGG	1 cut(s)	385
BsmAI	GTCTC	6 cut(s)	4, 85, 96, 516, 707, 835
BsmFI	GGGAC	1 cut(s)	772
BsmI	GAATGC	2 cut(s)	198, 212
BsnI	GGCC	3 cut(s)	177, 648, 774
Bso31I	GGTCTC	1 cut(s)	835
Bsp143I	GATC	2 cut(s)	334, 784
Bsp19I	CCATGG	1 cut(s)	346
BspACI	CCGC	2 cut(s)	359, 846
BspANI	GGCC	3 cut(s)	177, 648, 774
BspCNI	CTCAG	2 cut(s)	19, 836
BspFNI	CGCG	1 cut(s)	38
BspLI	GGNNCC	2 cut(s)	79, 760
BspTNI	GGTCTC	1 cut(s)	835
BsrFI	RCCGGY	1 cut(s)	761
BssAI	RCCGGY	1 cut(s)	761
BssECI	CCNNGG	1 cut(s)	346
BssMI	GATC	2 cut(s)	334, 784
BssSI	CACGAG	1 cut(s)	626
BssT1I	CCWWGG	1 cut(s)	346
Bst2BI	CACGAG	1 cut(s)	626
Bst2UI	CCWGG	1 cut(s)	380
Bst6I	CTCTTC	2 cut(s)	285, 435
BstAPI	GCANNNNNTGC	1 cut(s)	195
BstC8I	GCNNGC	2 cut(s)	212, 772
BstDEI	CTNAG	3 cut(s)	6, 635, 823
BstDSI	CCRYGG	1 cut(s)	346
BstF5I	GGATG	2 cut(s)	231, 725
BstFNI	CGCG	1 cut(s)	38
BstH2I	RGCGCY	1 cut(s)	449
BstHHI	GCGC	1 cut(s)	448
BstKTI	GATC	2 cut(s)	337, 787
BstMAI	GTCTC	6 cut(s)	4, 85, 96, 516, 707, 835
BstMBI	GATC	2 cut(s)	334, 784
BstMWI	GCNNNNNNNGC	4 cut(s)	174, 195, 645, 771
BstNI	CCWGG	1 cut(s)	380
BstSCI	CCNGG	2 cut(s)	378, 384
BstSFI	CTRYAG	1 cut(s)	687
BstUI	CGCG	1 cut(s)	38
BstV1I	GCAGC	2 cut(s)	459, 581
BstV2I	GAAGAC	2 cut(s)	543, 646
BsuRI	GGCC	3 cut(s)	177, 648, 774
BtgI	CCRYGG	1 cut(s)	346
BtrI	CACGTC	1 cut(s)	652
BtsCI	GGATG	2 cut(s)	231, 725
BtsIMutI	CAGTG	1 cut(s)	531
Cac8I	GCNNGC	2 cut(s)	212, 772
CfoI	GCGC	1 cut(s)	448
Cfr10I	RCCGGY	1 cut(s)	761
Cfr13I	GGNCC	2 cut(s)	382, 759
Csp6I	GTAC	1 cut(s)	356
CviAII	CATG	5 cut(s)	160, 179, 347, 404, 642
CviQI	GTAC	1 cut(s)	356
DdeI	CTNAG	3 cut(s)	6, 635, 823
DpnI	GATC	2 cut(s)	336, 786
DpnII	GATC	2 cut(s)	334, 784
EaeI	YGGCCR	2 cut(s)	646, 772
Eam1104I	CTCTTC	2 cut(s)	285, 435
EarI	CTCTTC	2 cut(s)	285, 435
Eco130I	CCWWGG	1 cut(s)	346
Eco31I	GGTCTC	1 cut(s)	835
Eco47I	GGWCC	2 cut(s)	382, 759
Eco57I	CTGAAG	2 cut(s)	527, 555
EcoRII	CCWGG	1 cut(s)	378
EcoT14I	CCWWGG	1 cut(s)	346
ErhI	CCWWGG	1 cut(s)	346
FaeI	CATG	5 cut(s)	163, 182, 350, 407, 645
FaiI	YATR	9 cut(s)	49, 75, 161, 180, 255, 348, 405, 643, 683
FalI	AAGNNNNNCTT	2 cut(s)	432, 464
FaqI	GGGAC	1 cut(s)	772
FatI	CATG	5 cut(s)	159, 178, 346, 403, 641
FauI	CCCGC	1 cut(s)	853
FbaI	TGATCA	1 cut(s)	334
Fnu4HI	GCNGC	3 cut(s)	473, 595, 849
FokI	GGATG	2 cut(s)	218, 732
Fsp4HI	GCNGC	3 cut(s)	473, 595, 849
GlaI	GCGC	1 cut(s)	447
GluI	GCNGC	3 cut(s)	473, 595, 849
HaeII	RGCGCY	1 cut(s)	449
HaeIII	GGCC	3 cut(s)	177, 648, 774
HapII	CCGG	2 cut(s)	385, 762
HhaI	GCGC	1 cut(s)	448
Hin1II	CATG	5 cut(s)	163, 182, 350, 407, 645
Hin6I	GCGC	1 cut(s)	446
HinP1I	GCGC	1 cut(s)	446
HindIII	AAGCTT	1 cut(s)	65
HinfI	GANTC	2 cut(s)	93, 104
HpaII	CCGG	2 cut(s)	385, 762
HphI	GGTGA	1 cut(s)	751
Hpy166II	GTNNAC	1 cut(s)	742
Hpy188I	TCNGA	3 cut(s)	9, 453, 591
Hpy188III	TCNNGA	3 cut(s)	203, 569, 788
Hpy8I	GTNNAC	1 cut(s)	742
Hpy99I	CGWCG	3 cut(s)	56, 551, 782
HpyAV	CCTTC	5 cut(s)	127, 157, 415, 454, 486
HpyCH4IV	ACGT	1 cut(s)	651
HpyCH4V	TGCA	7 cut(s)	189, 198, 251, 353, 597, 711, 723
HpyF10VI	GCNNNNNNNGC	4 cut(s)	174, 195, 645, 771
HpyF3I	CTNAG	3 cut(s)	6, 635, 823
HpySE526I	ACGT	1 cut(s)	651
Hsp92II	CATG	5 cut(s)	163, 182, 350, 407, 645
HspAI	GCGC	1 cut(s)	446
Ksp22I	TGATCA	1 cut(s)	334
Kzo9I	GATC	2 cut(s)	334, 784
Lsp1109I	GCAGC	2 cut(s)	459, 581
LweI	GCATC	2 cut(s)	112, 710
MaeII	ACGT	1 cut(s)	651
MaeIII	GTNAC	2 cut(s)	424, 601
MalI	GATC	2 cut(s)	336, 786
MboI	GATC	2 cut(s)	334, 784
MlsI	TGGCCA	2 cut(s)	648, 774
MluNI	TGGCCA	2 cut(s)	648, 774
MlyI	GAGTC	2 cut(s)	87, 98
MnlI	CCTC	8 cut(s)	15, 44, 78, 143, 319, 436, 576, 749
Mox20I	TGGCCA	2 cut(s)	648, 774
MroXI	GAANNNNTTC	1 cut(s)	146
MscI	TGGCCA	2 cut(s)	648, 774
MseI	TTAA	3 cut(s)	299, 608, 859
MslI	CAYNNNNRTG	2 cut(s)	366, 780
Msp20I	TGGCCA	2 cut(s)	648, 774
MspI	CCGG	2 cut(s)	385, 762
MspR9I	CCNGG	2 cut(s)	380, 386
Mva1269I	GAATGC	2 cut(s)	198, 212
MvaI	CCWGG	1 cut(s)	380
MvnI	CGCG	1 cut(s)	38
MwoI	GCNNNNNNNGC	4 cut(s)	174, 195, 645, 771
NciI	CCSGG	1 cut(s)	386
NcoI	CCATGG	1 cut(s)	346
NdeII	GATC	2 cut(s)	334, 784
NlaIII	CATG	5 cut(s)	163, 182, 350, 407, 645
NlaIV	GGNNCC	2 cut(s)	79, 760
NmeAIII	GCCGAG	1 cut(s)	75
NmuCI	GTSAC	1 cut(s)	424
PctI	GAATGC	2 cut(s)	198, 212
PdmI	GAANNNNTTC	1 cut(s)	146
PkrI	GCNGC	3 cut(s)	474, 596, 850
PleI	GAGTC	2 cut(s)	87, 98
PpsI	GAGTC	2 cut(s)	87, 98
Psp6I	CCWGG	1 cut(s)	378
PspGI	CCWGG	1 cut(s)	378
PspN4I	GGNNCC	2 cut(s)	79, 760
PspPI	GGNCC	2 cut(s)	382, 759
RsaI	GTAC	1 cut(s)	357
RsaNI	GTAC	1 cut(s)	356
RseI	CAYNNNNRTG	2 cut(s)	366, 780
SaqAI	TTAA	3 cut(s)	299, 608, 859
SatI	GCNGC	3 cut(s)	473, 595, 849
Sau3AI	GATC	2 cut(s)	334, 784
Sau96I	GGNCC	2 cut(s)	382, 759
SchI	GAGTC	2 cut(s)	87, 98
ScrFI	CCNGG	2 cut(s)	380, 386
SfaNI	GCATC	2 cut(s)	112, 710
SfcI	CTRYAG	1 cut(s)	687
SinI	GGWCC	2 cut(s)	382, 759
SmiMI	CAYNNNNRTG	2 cut(s)	366, 780
SmlI	CTYRAG	1 cut(s)	323
SmoI	CTYRAG	1 cut(s)	323
SsiI	CCGC	2 cut(s)	359, 846
StyD4I	CCNGG	2 cut(s)	378, 384
StyI	CCWWGG	1 cut(s)	346
TaiI	ACGT	1 cut(s)	654
TaqI	TCGA	4 cut(s)	17, 120, 153, 204
Tru1I	TTAA	3 cut(s)	299, 608, 859
Tru9I	TTAA	3 cut(s)	299, 608, 859
TscAI	CASTG	1 cut(s)	538
TseFI	GTSAC	1 cut(s)	424
TseI	GCWGC	3 cut(s)	472, 594, 848
Tsp45I	GTSAC	1 cut(s)	424
TspDTI	ATGAA	5 cut(s)	139, 300, 392, 473, 670
TspRI	CASTG	1 cut(s)	538
VpaK11BI	GGWCC	2 cut(s)	382, 759
XmnI	GAANNNNTTC	1 cut(s)	146

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.