MD05G1341900.v1.1

P21-Rho-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010970
Physical Location & Seq
Forward (+)
46242006 .. 46245138
3133 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1341900.v1.1.491

Sequence Viewer

Length: 450 bp
ATGGCAAAGATCTTGAAATACTTCTCTCAAATGTTTGTTGTGAAGGAGCGTGAAATGGAAATTGGGTTCCCAACAGATGTGAAACATGTAGCACACATTGGTTGGGACAGCCCGTCTGAAAGCGCGCCCAGTTGGATGAACGAATTTAAAAACGGACCCGATTTCTCAACGAGCTCCCATGGTCGGAATGCCAGTGATCGAATGGGGTCCTCTAATCCCATGGCTTATTCCCCTTGGTCCTCTCAAGATTTTGAACAACCCATAACGCGGCAACCATCATCCGACGTGTTCAGATATCTTTCTCCAACTGAACTTCCTAATGTTTCCAAGAAACCAAAGCGAAAAAAGGTCAAATCGTCATCTCCCTCGGCTGGAAGATCGTCACAAGGATCAAAGTCAAAGGCAACTGCATACACCCAAATGGAGGCAACACAAAATCGGATAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD

Protein Analysis

150

Amino Acids

16.7

Weight (kDa)

9.73

Isoelectric Point (pI)

59.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010970)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61795 AT4G04900 AT4G04900 AT4G21745
fragaria_vesca FvH4_3g02570 FvH4_3g02570
malus_domestica MD05G1341900.v1.1
prunus_persica Prupe.4G025100_v2.0.a1 Prupe.4G025100_v2.0.a1
pyrus_communis pycom05g31130
rosa_chinensis RchiOBHm_Chr5g0003861
rosa_laevigata RLG00000031184
rosa_multiflora Rmu_co8278535.1_g000001
rosa_roxburghii Rroxscaffold_1G00072110
rosa_rugosa Rorug04G0407500
rosa_samantha Rh5AG035000 Rh5BG033500 Rh5CG037400 Rh5DG033700
rosa_wichuraiana Rw5G003200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 125, 268
AciI CCGC 1 cut(s) 268
AclWI GGATC 1 cut(s) 397
AcsI RAATTY 1 cut(s) 143
AfiI CCNNNNNNNGG 4 cut(s) 183, 267, 371, 424
AflIII ACRYGT 2 cut(s) 85, 285
AgsI TTSAA 2 cut(s) 16, 254
AhdI GACNNNNNGTC 1 cut(s) 112
AjiI CACGTC 1 cut(s) 286
AjuI GAANNNNNNNTTGG 2 cut(s) 45, 77
AloI GAACNNNNNNTCC 2 cut(s) 50, 82
AluBI AGCT 1 cut(s) 174
AluI AGCT 1 cut(s) 174
Alw21I GWGCWC 1 cut(s) 176
AlwI GGATC 1 cut(s) 397
ApoI RAATTY 1 cut(s) 143
Asp700I GAANNNNTTC 1 cut(s) 20
AspLEI GCGC 2 cut(s) 125, 127
AspS9I GGNCC 3 cut(s) 155, 207, 237
AvaII GGWCC 3 cut(s) 155, 207, 237
BanII GRGCYC 1 cut(s) 176
Bbv12I GWGCWC 1 cut(s) 176
BccI CCATC 1 cut(s) 283
BglII AGATCT 1 cut(s) 9
BisI GCNGC 1 cut(s) 269
BlsI GCNGC 1 cut(s) 270
Bme18I GGWCC 3 cut(s) 155, 207, 237
BmeRI GACNNNNNGTC 1 cut(s) 112
BmgBI CACGTC 1 cut(s) 286
BmgT120I GGNCC 3 cut(s) 155, 207, 237
BmiI GGNNCC 3 cut(s) 68, 157, 208
BmrI ACTGGG 1 cut(s) 123
BmuI ACTGGG 1 cut(s) 123
BpuEI CTTGAG 1 cut(s) 228
BsaJI CCNNGG 4 cut(s) 178, 219, 233, 366
Bsc4I CCNNNNNNNGG 4 cut(s) 183, 267, 371, 424
Bse1I ACTGG 2 cut(s) 129, 192
BseDI CCNNGG 4 cut(s) 178, 219, 233, 366
BseGI GGATG 2 cut(s) 141, 278
BseLI CCNNNNNNNGG 4 cut(s) 183, 267, 371, 424
BseNI ACTGG 2 cut(s) 129, 192
BsePI GCGCGC 1 cut(s) 123
Bsh1236I CGCG 2 cut(s) 125, 268
BsiHKAI GWGCWC 1 cut(s) 176
BslFI GGGAC 1 cut(s) 119
BslI CCNNNNNNNGG 4 cut(s) 183, 267, 371, 424
BsmFI GGGAC 1 cut(s) 119
BsmI GAATGC 1 cut(s) 193
Bsp1286I GDGCHC 1 cut(s) 176
Bsp143I GATC 4 cut(s) 9, 196, 377, 389
Bsp19I CCATGG 2 cut(s) 178, 219
BspACI CCGC 1 cut(s) 268
BspFNI CGCG 2 cut(s) 125, 268
BspLI GGNNCC 3 cut(s) 68, 157, 208
BspPI GGATC 1 cut(s) 397
BsrI ACTGG 2 cut(s) 129, 192
BssECI CCNNGG 4 cut(s) 178, 219, 233, 366
BssHII GCGCGC 1 cut(s) 123
BssMI GATC 4 cut(s) 9, 196, 377, 389
BssT1I CCWWGG 3 cut(s) 178, 219, 233
BstC8I GCNNGC 1 cut(s) 125
BstDSI CCRYGG 2 cut(s) 178, 219
BstF5I GGATG 2 cut(s) 141, 278
BstFNI CGCG 2 cut(s) 125, 268
BstHHI GCGC 2 cut(s) 125, 127
BstKTI GATC 4 cut(s) 12, 199, 380, 392
BstMBI GATC 4 cut(s) 9, 196, 377, 389
BstNSI RCATGY 1 cut(s) 89
BstUI CGCG 2 cut(s) 125, 268
BstX2I RGATCY 1 cut(s) 9
BstYI RGATCY 1 cut(s) 9
BtgI CCRYGG 2 cut(s) 178, 219
BtrI CACGTC 1 cut(s) 286
BtsCI GGATG 2 cut(s) 141, 278
BtsIMutI CAGTG 1 cut(s) 199
Cac8I GCNNGC 1 cut(s) 125
CfoI GCGC 2 cut(s) 125, 127
Cfr13I GGNCC 3 cut(s) 155, 207, 237
CviAII CATG 3 cut(s) 86, 179, 220
CviJI RGCY 4 cut(s) 111, 174, 224, 371
CviKI_1 RGCY 4 cut(s) 111, 174, 224, 371
DpnI GATC 4 cut(s) 11, 198, 379, 391
DpnII GATC 4 cut(s) 9, 196, 377, 389
DraI TTTAAA 1 cut(s) 148
DriI GACNNNNNGTC 1 cut(s) 112
Eam1105I GACNNNNNGTC 1 cut(s) 112
Ecl136II GAGCTC 1 cut(s) 174
Eco130I CCWWGG 3 cut(s) 178, 219, 233
Eco24I GRGCYC 1 cut(s) 176
Eco32I GATATC 1 cut(s) 296
Eco47I GGWCC 3 cut(s) 155, 207, 237
Eco53kI GAGCTC 1 cut(s) 174
EcoICRI GAGCTC 1 cut(s) 174
EcoO109I RGGNCCY 1 cut(s) 207
EcoRV GATATC 1 cut(s) 296
EcoT14I CCWWGG 3 cut(s) 178, 219, 233
EcoT38I GRGCYC 1 cut(s) 176
ErhI CCWWGG 3 cut(s) 178, 219, 233
FaeI CATG 3 cut(s) 89, 182, 223
FaiI YATR 6 cut(s) 87, 180, 221, 263, 412, 448
FaqI GGGAC 1 cut(s) 119
FatI CATG 3 cut(s) 85, 178, 219
Fnu4HI GCNGC 1 cut(s) 269
FokI GGATG 2 cut(s) 148, 265
FriOI GRGCYC 1 cut(s) 176
Fsp4HI GCNGC 1 cut(s) 269
GlaI GCGC 2 cut(s) 124, 126
GluI GCNGC 1 cut(s) 269
HhaI GCGC 2 cut(s) 125, 127
Hin1II CATG 3 cut(s) 89, 182, 223
Hin6I GCGC 2 cut(s) 123, 125
HinP1I GCGC 2 cut(s) 123, 125
Hpy188I TCNGA 5 cut(s) 118, 186, 283, 293, 441
Hpy188III TCNNGA 2 cut(s) 13, 245
Hpy99I CGWCG 1 cut(s) 287
HpyAV CCTTC 1 cut(s) 37
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 1 cut(s) 410
HpySE526I ACGT 1 cut(s) 285
Hsp92II CATG 3 cut(s) 89, 182, 223
HspAI GCGC 2 cut(s) 123, 125
Kzo9I GATC 4 cut(s) 9, 196, 377, 389
LmnI GCTCC 2 cut(s) 46, 179
LpnPI CCDG 3 cut(s) 142, 205, 357
MaeII ACGT 1 cut(s) 285
MaeIII GTNAC 1 cut(s) 381
MalI GATC 4 cut(s) 11, 198, 379, 391
MboI GATC 4 cut(s) 9, 196, 377, 389
MboII GAAGA 1 cut(s) 387
MflI RGATCY 1 cut(s) 9
MhlI GDGCHC 1 cut(s) 176
MluCI AATT 2 cut(s) 60, 143
MmeI TCCRAC 4 cut(s) 113, 164, 306, 329
MnlI CCTC 4 cut(s) 220, 250, 376, 418
MroXI GAANNNNTTC 1 cut(s) 20
MseI TTAA 1 cut(s) 147
MslI CAYNNNNRTG 1 cut(s) 419
Mva1269I GAATGC 1 cut(s) 193
MvnI CGCG 2 cut(s) 125, 268
NcoI CCATGG 2 cut(s) 178, 219
NdeII GATC 4 cut(s) 9, 196, 377, 389
NlaIII CATG 3 cut(s) 89, 182, 223
NlaIV GGNNCC 3 cut(s) 68, 157, 208
NmeAIII GCCGAG 1 cut(s) 347
NmuCI GTSAC 1 cut(s) 381
NspI RCATGY 1 cut(s) 89
PauI GCGCGC 1 cut(s) 123
PciI ACATGT 1 cut(s) 85
PctI GAATGC 1 cut(s) 193
PdmI GAANNNNTTC 1 cut(s) 20
PkrI GCNGC 1 cut(s) 270
PpuMI RGGWCCY 1 cut(s) 207
PscI ACATGT 1 cut(s) 85
Psp124BI GAGCTC 1 cut(s) 176
Psp5II RGGWCCY 1 cut(s) 207
PspN4I GGNNCC 3 cut(s) 68, 157, 208
PspPI GGNCC 3 cut(s) 155, 207, 237
PspPPI RGGWCCY 1 cut(s) 207
PsuI RGATCY 1 cut(s) 9
PteI GCGCGC 1 cut(s) 123
RseI CAYNNNNRTG 1 cut(s) 419
SacI GAGCTC 1 cut(s) 176
SaqAI TTAA 1 cut(s) 147
SatI GCNGC 1 cut(s) 269
Sau3AI GATC 4 cut(s) 9, 196, 377, 389
Sau96I GGNCC 3 cut(s) 155, 207, 237
SduI GDGCHC 1 cut(s) 176
SetI ASST 3 cut(s) 176, 288, 351
SinI GGWCC 3 cut(s) 155, 207, 237
SmiMI CAYNNNNRTG 1 cut(s) 419
SmlI CTYRAG 1 cut(s) 243
SmoI CTYRAG 1 cut(s) 243
Sse9I AATT 2 cut(s) 60, 143
SsiI CCGC 1 cut(s) 268
SstI GAGCTC 1 cut(s) 176
StyI CCWWGG 3 cut(s) 178, 219, 233
TaiI ACGT 1 cut(s) 288
TaqI TCGA 1 cut(s) 199
TasI AATT 2 cut(s) 60, 143
TauI GCSGC 1 cut(s) 271
Tru1I TTAA 1 cut(s) 147
Tru9I TTAA 1 cut(s) 147
TscAI CASTG 1 cut(s) 199
TseFI GTSAC 1 cut(s) 381
Tsp45I GTSAC 1 cut(s) 381
TspDTI ATGAA 1 cut(s) 152
TspGWI ACGGA 1 cut(s) 168
TspRI CASTG 1 cut(s) 199
VpaK11BI GGWCC 3 cut(s) 155, 207, 237
XapI RAATTY 1 cut(s) 143
XceI RCATGY 1 cut(s) 89
XcmI CCANNNNNNNNNTGG 1 cut(s) 199
XmnI GAANNNNTTC 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.