pycom05g31130

P21-Rho-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0010970
Physical Location & Seq
Forward (+)
30977147 .. 30979112
1966 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g31130.4

Sequence Viewer

Length: 450 bp
ATGGCAAAGATCTTGAAATACTTCTCTCAAATGTTTGTTGTGAAGGAGCGTGAAATGAAAATTGGGTTCCCAACAGATGTGAAACACGTAGCACACATTGGTTGGGACGGCCCATCTGAAAGCGCACCCGGTTGGATGAGCGAATTTAAAAACGGACCCGATTTCTCAACGAGCTCCCATGGTCGTAATGCCAGTGATCGAATGGGGTCCTCTAATCCCATGGCTTATTCCCCTTGGTCCTCTCAAGATTTTGAACAACCCATAACACGGCAACCATCATCCGACATGTTCAGAAATCATCCTGCAACTGAACTTCCTAATGTGCCCAAGAAACCTAAGCGAAAAAAGGTCAAATCGTCATCTCCCTTGGCTGGAAGATCGTCACGAGGATCAAAGTCGAAGGCAACTGCATACACCCAAATGGAGGCAACACAAAATCTGCTAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD

Protein Analysis

150

Amino Acids

16.62

Weight (kDa)

9.94

Isoelectric Point (pI)

56.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010970)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61795 AT4G04900 AT4G04900 AT4G21745
fragaria_vesca FvH4_3g02570 FvH4_3g02570
malus_domestica MD05G1341900.v1.1
prunus_persica Prupe.4G025100_v2.0.a1 Prupe.4G025100_v2.0.a1
pyrus_communis pycom05g31130
rosa_chinensis RchiOBHm_Chr5g0003861
rosa_laevigata RLG00000031184
rosa_multiflora Rmu_co8278535.1_g000001
rosa_roxburghii Rroxscaffold_1G00072110
rosa_rugosa Rorug04G0407500
rosa_samantha Rh5AG035000 Rh5BG033500 Rh5CG037400 Rh5DG033700
rosa_wichuraiana Rw5G003200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 397
AcsI RAATTY 1 cut(s) 143
AfiI CCNNNNNNNGG 3 cut(s) 267, 371, 424
AflIII ACRYGT 2 cut(s) 85, 285
AgsI TTSAA 2 cut(s) 16, 254
AjuI GAANNNNNNNTTGG 2 cut(s) 45, 77
AluBI AGCT 1 cut(s) 174
AluI AGCT 1 cut(s) 174
Alw21I GWGCWC 1 cut(s) 176
AlwI GGATC 1 cut(s) 397
AoxI GGCC 1 cut(s) 109
ApoI RAATTY 1 cut(s) 143
Asp700I GAANNNNTTC 1 cut(s) 20
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 4 cut(s) 110, 155, 207, 237
AsuC2I CCSGG 1 cut(s) 129
AvaII GGWCC 3 cut(s) 155, 207, 237
BaeGI GKGCMC 1 cut(s) 327
BanII GRGCYC 1 cut(s) 176
BauI CACGAG 1 cut(s) 384
Bbv12I GWGCWC 1 cut(s) 176
BccI CCATC 2 cut(s) 121, 283
BceAI ACGGC 2 cut(s) 124, 284
BcnI CCSGG 1 cut(s) 129
BglII AGATCT 1 cut(s) 9
Bme1390I CCNGG 1 cut(s) 129
Bme18I GGWCC 3 cut(s) 155, 207, 237
BmgT120I GGNCC 4 cut(s) 110, 155, 207, 237
BmiI GGNNCC 3 cut(s) 68, 157, 208
BmrFI CCNGG 1 cut(s) 129
Bpu10I CCTNAGC 1 cut(s) 336
BpuEI CTTGAG 1 cut(s) 228
BpuMI CCSGG 1 cut(s) 129
BsaAI YACGTR 1 cut(s) 88
BsaJI CCNNGG 4 cut(s) 178, 219, 233, 366
Bsc4I CCNNNNNNNGG 3 cut(s) 267, 371, 424
Bse1I ACTGG 1 cut(s) 192
BseDI CCNNGG 4 cut(s) 178, 219, 233, 366
BseGI GGATG 3 cut(s) 141, 278, 298
BseLI CCNNNNNNNGG 3 cut(s) 267, 371, 424
BseNI ACTGG 1 cut(s) 192
BseSI GKGCMC 1 cut(s) 327
BshFI GGCC 1 cut(s) 111
BsiHKAI GWGCWC 1 cut(s) 176
BsiSI CCGG 1 cut(s) 129
BslFI GGGAC 1 cut(s) 119
BslI CCNNNNNNNGG 3 cut(s) 267, 371, 424
BsmFI GGGAC 1 cut(s) 119
BsnI GGCC 1 cut(s) 111
Bsp1286I GDGCHC 2 cut(s) 176, 327
Bsp143I GATC 4 cut(s) 9, 196, 377, 389
Bsp19I CCATGG 2 cut(s) 178, 219
BspANI GGCC 1 cut(s) 111
BspLI GGNNCC 3 cut(s) 68, 157, 208
BspPI GGATC 1 cut(s) 397
BsrI ACTGG 1 cut(s) 192
BssECI CCNNGG 4 cut(s) 178, 219, 233, 366
BssMI GATC 4 cut(s) 9, 196, 377, 389
BssSI CACGAG 1 cut(s) 384
BssT1I CCWWGG 4 cut(s) 178, 219, 233, 366
Bst2BI CACGAG 1 cut(s) 384
BstBAI YACGTR 1 cut(s) 88
BstDEI CTNAG 1 cut(s) 336
BstDSI CCRYGG 2 cut(s) 178, 219
BstF5I GGATG 3 cut(s) 141, 278, 298
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 4 cut(s) 12, 199, 380, 392
BstMBI GATC 4 cut(s) 9, 196, 377, 389
BstNSI RCATGY 1 cut(s) 289
BstSCI CCNGG 1 cut(s) 127
BstSLI GKGCMC 1 cut(s) 327
BstX2I RGATCY 1 cut(s) 9
BstYI RGATCY 1 cut(s) 9
BsuRI GGCC 1 cut(s) 111
BtgI CCRYGG 2 cut(s) 178, 219
BtsCI GGATG 3 cut(s) 141, 278, 298
BtsIMutI CAGTG 1 cut(s) 199
CfoI GCGC 1 cut(s) 125
Cfr13I GGNCC 4 cut(s) 110, 155, 207, 237
CviAII CATG 3 cut(s) 179, 220, 286
CviJI RGCY 4 cut(s) 111, 174, 224, 371
CviKI_1 RGCY 4 cut(s) 111, 174, 224, 371
DdeI CTNAG 1 cut(s) 336
DpnI GATC 4 cut(s) 11, 198, 379, 391
DpnII GATC 4 cut(s) 9, 196, 377, 389
DraI TTTAAA 1 cut(s) 148
Ecl136II GAGCTC 1 cut(s) 174
Eco130I CCWWGG 4 cut(s) 178, 219, 233, 366
Eco24I GRGCYC 1 cut(s) 176
Eco47I GGWCC 3 cut(s) 155, 207, 237
Eco53kI GAGCTC 1 cut(s) 174
EcoICRI GAGCTC 1 cut(s) 174
EcoO109I RGGNCCY 1 cut(s) 207
EcoT14I CCWWGG 4 cut(s) 178, 219, 233, 366
EcoT38I GRGCYC 1 cut(s) 176
ErhI CCWWGG 4 cut(s) 178, 219, 233, 366
FaeI CATG 3 cut(s) 182, 223, 289
FaiI YATR 6 cut(s) 180, 221, 263, 287, 412, 448
FaqI GGGAC 1 cut(s) 119
FatI CATG 3 cut(s) 178, 219, 285
FokI GGATG 3 cut(s) 148, 265, 285
FriOI GRGCYC 1 cut(s) 176
GlaI GCGC 1 cut(s) 124
HaeIII GGCC 1 cut(s) 111
HapII CCGG 1 cut(s) 129
HhaI GCGC 1 cut(s) 125
Hin1II CATG 3 cut(s) 182, 223, 289
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HpaII CCGG 1 cut(s) 129
Hpy188I TCNGA 3 cut(s) 118, 283, 293
Hpy188III TCNNGA 3 cut(s) 13, 245, 384
HpyAV CCTTC 2 cut(s) 37, 394
HpyCH4IV ACGT 1 cut(s) 87
HpyCH4V TGCA 2 cut(s) 305, 410
HpyF3I CTNAG 1 cut(s) 336
HpySE526I ACGT 1 cut(s) 87
Hsp92II CATG 3 cut(s) 182, 223, 289
HspAI GCGC 1 cut(s) 123
Kzo9I GATC 4 cut(s) 9, 196, 377, 389
LmnI GCTCC 2 cut(s) 46, 179
LpnPI CCDG 4 cut(s) 142, 205, 315, 357
MaeII ACGT 1 cut(s) 87
MaeIII GTNAC 1 cut(s) 381
MalI GATC 4 cut(s) 11, 198, 379, 391
MboI GATC 4 cut(s) 9, 196, 377, 389
MboII GAAGA 1 cut(s) 387
MflI RGATCY 1 cut(s) 9
MhlI GDGCHC 2 cut(s) 176, 327
MluCI AATT 2 cut(s) 60, 143
MmeI TCCRAC 2 cut(s) 113, 306
MnlI CCTC 4 cut(s) 220, 250, 380, 418
MroXI GAANNNNTTC 1 cut(s) 20
MseI TTAA 1 cut(s) 147
MslI CAYNNNNRTG 1 cut(s) 419
MspI CCGG 1 cut(s) 129
MspR9I CCNGG 1 cut(s) 129
NciI CCSGG 1 cut(s) 129
NcoI CCATGG 2 cut(s) 178, 219
NdeII GATC 4 cut(s) 9, 196, 377, 389
NlaIII CATG 3 cut(s) 182, 223, 289
NlaIV GGNNCC 3 cut(s) 68, 157, 208
NmuCI GTSAC 1 cut(s) 381
NspI RCATGY 1 cut(s) 289
PciI ACATGT 1 cut(s) 285
PdmI GAANNNNTTC 1 cut(s) 20
Ppu21I YACGTR 1 cut(s) 88
PpuMI RGGWCCY 1 cut(s) 207
PscI ACATGT 1 cut(s) 285
Psp124BI GAGCTC 1 cut(s) 176
Psp5II RGGWCCY 1 cut(s) 207
PspN4I GGNNCC 3 cut(s) 68, 157, 208
PspPI GGNCC 4 cut(s) 110, 155, 207, 237
PspPPI RGGWCCY 1 cut(s) 207
PsuI RGATCY 1 cut(s) 9
RseI CAYNNNNRTG 1 cut(s) 419
SacI GAGCTC 1 cut(s) 176
SaqAI TTAA 1 cut(s) 147
Sau3AI GATC 4 cut(s) 9, 196, 377, 389
Sau96I GGNCC 4 cut(s) 110, 155, 207, 237
ScrFI CCNGG 1 cut(s) 129
SduI GDGCHC 2 cut(s) 176, 327
SetI ASST 4 cut(s) 90, 176, 337, 351
SinI GGWCC 3 cut(s) 155, 207, 237
SmiMI CAYNNNNRTG 1 cut(s) 419
SmlI CTYRAG 1 cut(s) 243
SmoI CTYRAG 1 cut(s) 243
Sse9I AATT 2 cut(s) 60, 143
SstI GAGCTC 1 cut(s) 176
StyD4I CCNGG 1 cut(s) 127
StyI CCWWGG 4 cut(s) 178, 219, 233, 366
TaiI ACGT 1 cut(s) 90
TaqI TCGA 2 cut(s) 199, 398
TasI AATT 2 cut(s) 60, 143
Tru1I TTAA 1 cut(s) 147
Tru9I TTAA 1 cut(s) 147
TscAI CASTG 1 cut(s) 199
TseFI GTSAC 1 cut(s) 381
Tsp45I GTSAC 1 cut(s) 381
TspDTI ATGAA 1 cut(s) 71
TspGWI ACGGA 1 cut(s) 168
TspRI CASTG 1 cut(s) 199
VpaK11BI GGWCC 3 cut(s) 155, 207, 237
XapI RAATTY 1 cut(s) 143
XceI RCATGY 1 cut(s) 289
XcmI CCANNNNNNNNNTGG 1 cut(s) 199
XmnI GAANNNNTTC 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.