MD05G1343200.v1.1

Chaperone protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008846
Physical Location & Seq
Forward (+)
46341186 .. 46345644
4459 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1343200.v1.1.491

Sequence Viewer

Length: 1986 bp
ATGGGGGAGTTTGTTAAGAAGCTCCAGGTTGAAGATCAGGAGAAGTTGGAGCTGGCTTATGGTGTTGAAATCGAAGCAGAGGCAGTGTGTGTTGCTTTCCGAATCATCATGCAATACCCCGATGTTTTTCAGTTCGATAGTAGTGGAGAATTTGATGACACCCAGAAGGAAATTTTGCAAAATAAGGTTGTTGGGCTTTTAAAGGAAGCTTGTGCAAAATTGAGGGATAAATTTGAGTACTCTAGACAAGAAGAGGATGATCTAGATGTCGGTGAGTACCAGTTGCGTCGCACACTTGTTGAGTTAAACGAAATCAAGAAAGAAAGAGATCCATCTACACAAAAGTGGAGCGATCGTGTGAAAAAGGAACATGAAGAATTGAAGGGTATGTGGAACCAGTTGGAGACGAACTGGGTGCAAGTAAAAACTTCACGGCAGAGATGTGACATCAGAGCTCGAGAGGCGCTTTTGTTGGAAAGGATGCACGAAAGGTTGGCTTTACGTATAAATGAGGCAAGATTAAAGACGGTGAACAGTTTCTCTGGGTCTGGGATGTTGCAATGGATTGATGACCATGTGACTGAGCTTTTATATACAACTCGCAAAGATTTTAAGCATGAGCTAACCGTTAGCCCGCACGTTGTTGCGAAGGCAGCAAGCCCCTTGATTGATGCTCCACCACCATTGTCGCTTTCTGAACCAACACTGCCTCAGAACCTTGAACAAAGAATTGCTAAGAGGGTAATTGGACAGGAACACGTTCTACTTGCAGTAACTGCTGCTCTATCAAGGCAGAGGCCTCAGCGACGCCCTATTGGATCCTTCCTCTTCATGTGCGGCTCTGGTTATGGGAGGACAGAAATGGCAAAATCTTTGGCCGGACTACTTTTTGATAACAAAGACATGATAGCAGAGTTTGACCTGGCAAAATACTCAGGTCCAAATTCTTTCTCACGCCTCATTGGTCTTCTTTCTAGTTACATTGAGTCAGGCATGAAGAGGGAGCGCTCAGAGGCTGTTAAGAAGAGGCCTATAGGTCTCATTTTGTTTGACAATGTTGACAAGGCTCATGGATCTATTATTGACATTCTGACCGAGATTATAGGTTTTGGTCATCTAATAGATGGTCAGGGGAGCGTCGTTGACTTCACTAAAACATTGATAATCATGACAACGAATATTGGATGTGATAAATACTGGCCATGGAATTGCAAATGTGCAGACGAAGTTCAGAAGTTCCCCTTAAAAGAAGGATTATATGATGATGCATGGGAAACAAAACACAATTCCTGCTATCTTTCTCTTTTGAGGGAGGCAAAACAGCATTTCAGACCTCAATTGCTTGAATATGTGGATGATGTGATTGGAATTAGGTCTCTATCCGTTCAGCAGTTGAAGGCGGTTGCTAGGCTCCAGTTAAGGGACATTGCCAGCTGCATGACACAGAAGGGCCTCATTATATACCCATCTGAAGCTGCATTAGATATTATTGTCCAGAGGTCAACTTGGCTTGGAGATAGAATCAGAGGTGGAGAGAGAATAAGGATGTGGCTTGAAGAGAACTTGGTTCCGCTGCTGTTTGAGAAGCTTGCTAAGAACATTTTAAATGAGATGTCTATCATATATATTGAAGCCTCGGTGGAGACAAACCAATTGTCCTACAGCTGGGCCAATTGTGTACATTCACTGGAAGAACAAGATGTTAACCAATTAGTATCTTTAAGGGATTTGAGATTGATGTATCGCAAAGAGAAAGAACGAGCAAAGAATGTCTATGTTCTAAGAAAACTTCACAACAGATTAATCGCAGGTGCAAATGCTGAACTGGGTTATACTACTGCTGTTGTTCAAGAGCTGGTTAATACAATTCAGGATCTAGTTACAATCCCCAGCAGTCTCAAGTCTTTGCTCGACAATCCTAAAATGGCGGCGGAGGCAGCCTTGAACGAGGACGAGGTTAGGCAGAACAAGAGGGCTAAAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04213 map04213
Pfam Domains
Protein Families
AAA_2

Protein Analysis

662

Amino Acids

75.66

Weight (kDa)

6.27

Isoelectric Point (pI)

41.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_2 PF07724 270 - 449 7.9e-26 AAA domain (Cdc48 subfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1799
Acc36I ACCTGC 1 cut(s) 1799
AciI CCGC 6 cut(s) 635, 837, 1400, 1571, 1928, 1931
AclWI GGATC 5 cut(s) 323, 813, 826, 1081, 1881
AcoI YGGCCR 2 cut(s) 876, 1199
AcsI RAATTY 4 cut(s) 149, 171, 230, 943
AcuI CTGAAG 1 cut(s) 1491
AcyI GRCGYC 1 cut(s) 808
AfaI GTAC 3 cut(s) 239, 278, 1680
AfeI AGCGCT 1 cut(s) 1007
AfiI CCNNNNNNNGG 2 cut(s) 1420, 1665
AflIII ACRYGT 1 cut(s) 757
AjnI CCWGG 2 cut(s) 24, 921
AleI CACNNNNGTG 1 cut(s) 343
Alw21I GWGCWC 1 cut(s) 457
Alw26I GTCTC 5 cut(s) 398, 1043, 1380, 1637, 1901
AlwI GGATC 5 cut(s) 323, 813, 826, 1081, 1881
AlwNI CAGNNNCTG 2 cut(s) 776, 1016
Ama87I CYCGRG 1 cut(s) 456
Aor51HI AGCGCT 1 cut(s) 1007
AoxI GGCC 6 cut(s) 797, 876, 1028, 1199, 1450, 1668
ApeKI GCWGC 6 cut(s) 653, 779, 1434, 1475, 1573, 1937
ApoI RAATTY 4 cut(s) 149, 171, 230, 943
ArsI GACNNNNNNTTYG 2 cut(s) 1640, 1672
AseI ATTAAT 1 cut(s) 1802
Asp700I GAANNNNTTC 2 cut(s) 536, 759
AspLEI GCGC 2 cut(s) 466, 1008
AspS9I GGNCC 3 cut(s) 938, 1450, 1668
AsuHPI GGTGA 2 cut(s) 284, 541
AvaI CYCGRG 1 cut(s) 456
AvaII GGWCC 1 cut(s) 938
BalI TGGCCA 1 cut(s) 1201
BamHI GGATCC 1 cut(s) 818
BanII GRGCYC 1 cut(s) 457
BbsI GAAGAC 1 cut(s) 959
Bbv12I GWGCWC 1 cut(s) 457
BbvCI CCTCAGC 1 cut(s) 801
BbvI GCAGC 6 cut(s) 665, 766, 1421, 1462, 1560, 1949
BccI CCATC 3 cut(s) 340, 1118, 1474
BceAI ACGGC 1 cut(s) 449
BcgI CGANNNNNNTGC 2 cut(s) 397, 431
BciT130I CCWGG 2 cut(s) 26, 923
BcoDI GTCTC 5 cut(s) 398, 1043, 1380, 1637, 1901
BfaI CTAG 5 cut(s) 243, 263, 975, 1407, 1877
BfmI CTRYAG 2 cut(s) 1032, 1660
BfoI RGCGCY 2 cut(s) 467, 1009
BfuAI ACCTGC 1 cut(s) 1799
BisI GCNGC 8 cut(s) 654, 780, 838, 1435, 1476, 1574, 1929, 1938
BlsI GCNGC 8 cut(s) 655, 781, 839, 1436, 1477, 1575, 1930, 1939
BmcAI AGTACT 1 cut(s) 239
Bme1390I CCNGG 2 cut(s) 26, 923
Bme18I GGWCC 1 cut(s) 938
BmeT110I CYCGRG 1 cut(s) 456
BmgT120I GGNCC 3 cut(s) 938, 1450, 1668
BmiI GGNNCC 4 cut(s) 395, 820, 1412, 1569
BmrFI CCNGG 2 cut(s) 26, 923
BmrI ACTGGG 2 cut(s) 421, 1835
BmsI GCATC 3 cut(s) 471, 661, 1255
BmuI ACTGGG 2 cut(s) 421, 1835
BpiI GAAGAC 1 cut(s) 959
BpmI CTGGAG 2 cut(s) 8, 1397
Bpu10I CCTNAGC 1 cut(s) 801
BpuEI CTTGAG 1 cut(s) 1883
BsaAI YACGTR 1 cut(s) 503
BsaBI GATNNNNATC 1 cut(s) 1616
BsaHI GRCGYC 1 cut(s) 808
BsaI GGTCTC 2 cut(s) 1043, 1380
BsaJI CCNNGG 2 cut(s) 1202, 1635
BsaXI ACNNNNNCTCC 2 cut(s) 340, 370
Bsc4I CCNNNNNNNGG 2 cut(s) 1420, 1665
Bse1I ACTGG 7 cut(s) 280, 397, 416, 1202, 1414, 1692, 1830
Bse3DI GCAATG 2 cut(s) 566, 1425
Bse8I GATNNNNATC 1 cut(s) 1616
BseBI CCWGG 2 cut(s) 26, 923
BseDI CCNNGG 2 cut(s) 1202, 1635
BseGI GGATG 6 cut(s) 262, 486, 558, 1190, 1360, 1551
BseJI GATNNNNATC 1 cut(s) 1616
BseLI CCNNNNNNNGG 2 cut(s) 1420, 1665
BseMI GCAATG 2 cut(s) 566, 1425
BseMII CTCAG 5 cut(s) 573, 725, 815, 948, 1023
BseNI ACTGG 7 cut(s) 280, 397, 416, 1202, 1414, 1692, 1830
BseXI GCAGC 6 cut(s) 665, 766, 1421, 1462, 1560, 1949
BseYI CCCAGC 2 cut(s) 1665, 1889
BsgI GTGCAG 1 cut(s) 1239
Bsh1285I CGRYCG 1 cut(s) 355
BshFI GGCC 6 cut(s) 799, 878, 1030, 1201, 1452, 1670
BsiEI CGRYCG 1 cut(s) 355
BsiHKAI GWGCWC 1 cut(s) 457
BsiHKCI CYCGRG 1 cut(s) 456
BsiSI CCGG 1 cut(s) 879
BslFI GGGAC 1 cut(s) 1436
BslI CCNNNNNNNGG 2 cut(s) 1420, 1665
BsmAI GTCTC 5 cut(s) 398, 1043, 1380, 1637, 1901
BsmBI CGTCTC 1 cut(s) 398
BsmFI GGGAC 1 cut(s) 1436
BsnI GGCC 6 cut(s) 799, 878, 1030, 1201, 1452, 1670
Bso31I GGTCTC 2 cut(s) 1043, 1380
BsoBI CYCGRG 1 cut(s) 456
Bsp1286I GDGCHC 1 cut(s) 457
Bsp1407I TGTACA 1 cut(s) 1678
Bsp143I GATC 7 cut(s) 34, 259, 328, 352, 818, 1073, 1873
Bsp19I CCATGG 1 cut(s) 1202
BspACI CCGC 6 cut(s) 635, 837, 1400, 1571, 1928, 1931
BspANI GGCC 6 cut(s) 799, 878, 1030, 1201, 1452, 1670
BspCNI CTCAG 5 cut(s) 574, 724, 814, 947, 1022
BspHI TCATGA 1 cut(s) 1167
BspLI GGNNCC 4 cut(s) 395, 820, 1412, 1569
BspMI ACCTGC 1 cut(s) 1799
BspPI GGATC 5 cut(s) 323, 813, 826, 1081, 1881
BspTNI GGTCTC 2 cut(s) 1043, 1380
BsrDI GCAATG 2 cut(s) 566, 1425
BsrGI TGTACA 1 cut(s) 1678
BsrI ACTGG 7 cut(s) 280, 397, 416, 1202, 1414, 1692, 1830
BssECI CCNNGG 2 cut(s) 1202, 1635
BssMI GATC 7 cut(s) 34, 259, 328, 352, 818, 1073, 1873
BssNI GRCGYC 1 cut(s) 808
BssT1I CCWWGG 1 cut(s) 1202
Bst2UI CCWGG 2 cut(s) 26, 923
Bst4CI ACNGT 3 cut(s) 529, 536, 628
Bst6I CTCTTC 5 cut(s) 246, 833, 992, 1019, 1551
BstACI GRCGYC 1 cut(s) 808
BstAPI GCANNNNNTGC 1 cut(s) 776
BstAUI TGTACA 1 cut(s) 1678
BstBAI YACGTR 1 cut(s) 503
BstC8I GCNNGC 5 cut(s) 54, 635, 658, 1432, 1590
BstDEI CTNAG 8 cut(s) 582, 711, 735, 801, 934, 1009, 1593, 1781
BstDSI CCRYGG 1 cut(s) 1202
BstF5I GGATG 6 cut(s) 262, 486, 558, 1190, 1360, 1551
BstH2I RGCGCY 2 cut(s) 467, 1009
BstHHI GCGC 2 cut(s) 466, 1008
BstKTI GATC 7 cut(s) 37, 262, 331, 355, 821, 1076, 1876
BstMAI GTCTC 5 cut(s) 398, 1043, 1380, 1637, 1901
BstMBI GATC 7 cut(s) 34, 259, 328, 352, 818, 1073, 1873
BstMCI CGRYCG 1 cut(s) 355
BstMWI GCNNNNNNNGC 5 cut(s) 461, 653, 776, 1934, 1937
BstNI CCWGG 2 cut(s) 26, 923
BstSCI CCNGG 2 cut(s) 24, 921
BstSFI CTRYAG 2 cut(s) 1032, 1660
BstSNI TACGTA 1 cut(s) 503
BstV1I GCAGC 6 cut(s) 665, 766, 1421, 1462, 1560, 1949
BstV2I GAAGAC 1 cut(s) 959
BstX2I RGATCY 4 cut(s) 328, 818, 1073, 1873
BstYI RGATCY 4 cut(s) 328, 818, 1073, 1873
BsuRI GGCC 6 cut(s) 799, 878, 1030, 1201, 1452, 1670
BtgI CCRYGG 1 cut(s) 1202
BtsCI GGATG 6 cut(s) 262, 486, 558, 1190, 1360, 1551
BtsI GCAGTG 2 cut(s) 90, 704
BtsIMutI CAGTG 3 cut(s) 90, 704, 1685
BveI ACCTGC 1 cut(s) 1799
Cac8I GCNNGC 5 cut(s) 54, 635, 658, 1432, 1590
CaiI CAGNNNCTG 2 cut(s) 776, 1016
CciI TCATGA 1 cut(s) 1167
CfoI GCGC 2 cut(s) 466, 1008
Cfr13I GGNCC 3 cut(s) 938, 1450, 1668
CseI GACGC 3 cut(s) 275, 816, 1126
Csp6I GTAC 3 cut(s) 238, 277, 1679
CspCI CAANNNNNGTGG 2 cut(s) 666, 701
CviQI GTAC 3 cut(s) 238, 277, 1679
DdeI CTNAG 8 cut(s) 582, 711, 735, 801, 934, 1009, 1593, 1781
DpnI GATC 7 cut(s) 36, 261, 330, 354, 820, 1075, 1875
DpnII GATC 7 cut(s) 34, 259, 328, 352, 818, 1073, 1873
DraI TTTAAA 2 cut(s) 201, 1605
EaeI YGGCCR 2 cut(s) 876, 1199
Eam1104I CTCTTC 5 cut(s) 246, 833, 992, 1019, 1551
EarI CTCTTC 5 cut(s) 246, 833, 992, 1019, 1551
EciI GGCGGA 1 cut(s) 1946
Ecl136II GAGCTC 1 cut(s) 455
Eco105I TACGTA 1 cut(s) 503
Eco130I CCWWGG 1 cut(s) 1202
Eco147I AGGCCT 2 cut(s) 799, 1030
Eco24I GRGCYC 1 cut(s) 457
Eco31I GGTCTC 2 cut(s) 1043, 1380
Eco47I GGWCC 1 cut(s) 938
Eco47III AGCGCT 1 cut(s) 1007
Eco53kI GAGCTC 1 cut(s) 455
Eco57I CTGAAG 1 cut(s) 1491
Eco88I CYCGRG 1 cut(s) 456
EcoICRI GAGCTC 1 cut(s) 455
EcoO109I RGGNCCY 1 cut(s) 1450
EcoRII CCWGG 2 cut(s) 24, 921
EcoT14I CCWWGG 1 cut(s) 1202
EcoT22I ATGCAT 1 cut(s) 1270
EcoT38I GRGCYC 1 cut(s) 457
ErhI CCWWGG 1 cut(s) 1202
Esp3I CGTCTC 1 cut(s) 398
FalI AAGNNNNNCTT 4 cut(s) 481, 513, 1226, 1258
FaqI GGGAC 1 cut(s) 1436
FauI CCCGC 1 cut(s) 642
Fnu4HI GCNGC 8 cut(s) 654, 780, 838, 1435, 1476, 1574, 1929, 1938
FokI GGATG 6 cut(s) 269, 493, 565, 1197, 1367, 1558
FriOI GRGCYC 1 cut(s) 457
Fsp4HI GCNGC 8 cut(s) 654, 780, 838, 1435, 1476, 1574, 1929, 1938
FspBI CTAG 5 cut(s) 243, 263, 975, 1407, 1877
GlaI GCGC 2 cut(s) 465, 1007
GluI GCNGC 8 cut(s) 654, 780, 838, 1435, 1476, 1574, 1929, 1938
GsaI CCCAGC 2 cut(s) 1669, 1893
GsuI CTGGAG 2 cut(s) 8, 1397
HaeII RGCGCY 2 cut(s) 467, 1009
HaeIII GGCC 6 cut(s) 799, 878, 1030, 1201, 1452, 1670
HapII CCGG 1 cut(s) 879
HgaI GACGC 3 cut(s) 275, 816, 1126
HhaI GCGC 2 cut(s) 466, 1008
Hin1I GRCGYC 1 cut(s) 808
Hin6I GCGC 2 cut(s) 464, 1006
HinP1I GCGC 2 cut(s) 464, 1006
HincII GTYRAC 4 cut(s) 1060, 1144, 1503, 1705
HindII GTYRAC 4 cut(s) 1060, 1144, 1503, 1705
HindIII AAGCTT 2 cut(s) 207, 1586
HinfI GANTC 3 cut(s) 102, 986, 1521
HpaI GTTAAC 1 cut(s) 1705
HpaII CCGG 1 cut(s) 879
HphI GGTGA 2 cut(s) 284, 541
Hpy166II GTNNAC 6 cut(s) 532, 1060, 1144, 1503, 1679, 1705
Hpy188III TCNNGA 9 cut(s) 38, 243, 263, 316, 458, 1168, 1495, 1850, 1871
Hpy8I GTNNAC 6 cut(s) 532, 1060, 1144, 1503, 1679, 1705
Hpy99I CGWCG 3 cut(s) 291, 810, 1142
HpyAV CCTTC 7 cut(s) 160, 376, 643, 832, 1244, 1390, 1441
HpyCH4III ACNGT 3 cut(s) 529, 536, 628
HpyCH4IV ACGT 3 cut(s) 502, 639, 759
HpyF10VI GCNNNNNNNGC 5 cut(s) 461, 653, 776, 1934, 1937
HpyF3I CTNAG 8 cut(s) 582, 711, 735, 801, 934, 1009, 1593, 1781
HpySE526I ACGT 3 cut(s) 502, 639, 759
Hsp92I GRCGYC 1 cut(s) 808
HspAI GCGC 2 cut(s) 464, 1006
KspAI GTTAAC 1 cut(s) 1705
Kzo9I GATC 7 cut(s) 34, 259, 328, 352, 818, 1073, 1873
LmnI GCTCC 7 cut(s) 27, 49, 348, 679, 1003, 1134, 1416
Lsp1109I GCAGC 6 cut(s) 665, 766, 1421, 1462, 1560, 1949
LweI GCATC 3 cut(s) 471, 661, 1255
MaeI CTAG 5 cut(s) 243, 263, 975, 1407, 1877
MaeII ACGT 3 cut(s) 502, 639, 759
MaeIII GTNAC 5 cut(s) 443, 577, 772, 977, 1879
MalI GATC 7 cut(s) 36, 261, 330, 354, 820, 1075, 1875
MboI GATC 7 cut(s) 34, 259, 328, 352, 818, 1073, 1873
MboII GAAGA 9 cut(s) 44, 263, 386, 820, 959, 1009, 1036, 1568, 1703
MfeI CAATTG 3 cut(s) 1337, 1652, 1672
MflI RGATCY 4 cut(s) 328, 818, 1073, 1873
MhlI GDGCHC 1 cut(s) 457
MlsI TGGCCA 1 cut(s) 1201
MluNI TGGCCA 1 cut(s) 1201
MlyI GAGTC 1 cut(s) 995
MmeI TCCRAC 3 cut(s) 27, 381, 453
Mox20I TGGCCA 1 cut(s) 1201
Mph1103I ATGCAT 1 cut(s) 1270
MroXI GAANNNNTTC 2 cut(s) 536, 759
MscI TGGCCA 1 cut(s) 1201
MslI CAYNNNNRTG 1 cut(s) 343
Msp20I TGGCCA 1 cut(s) 1201
MspA1I CMGCKG 3 cut(s) 1434, 1573, 1665
MspI CCGG 1 cut(s) 879
MspR9I CCNGG 2 cut(s) 26, 923
MunI CAATTG 3 cut(s) 1337, 1652, 1672
MvaI CCWGG 2 cut(s) 26, 923
MwoI GCNNNNNNNGC 5 cut(s) 461, 653, 776, 1934, 1937
NcoI CCATGG 1 cut(s) 1202
NdeII GATC 7 cut(s) 34, 259, 328, 352, 818, 1073, 1873
NlaIV GGNNCC 4 cut(s) 395, 820, 1412, 1569
NmuCI GTSAC 2 cut(s) 443, 577
NsiI ATGCAT 1 cut(s) 1270
OliI CACNNNNGTG 1 cut(s) 343
PaeR7I CTCGAG 1 cut(s) 456
PagI TCATGA 1 cut(s) 1167
PaqCI CACCTGC 1 cut(s) 1799
PceI AGGCCT 2 cut(s) 799, 1030
PdmI GAANNNNTTC 2 cut(s) 536, 759
PfeI GAWTC 2 cut(s) 102, 1521
PkrI GCNGC 8 cut(s) 655, 781, 839, 1436, 1477, 1575, 1930, 1939
Ple19I CGATCG 1 cut(s) 355
PleI GAGTC 1 cut(s) 994
PpsI GAGTC 1 cut(s) 994
Ppu21I YACGTR 1 cut(s) 503
PshBI ATTAAT 1 cut(s) 1802
Psp124BI GAGCTC 1 cut(s) 457
Psp6I CCWGG 2 cut(s) 24, 921
PspFI CCCAGC 2 cut(s) 1665, 1889
PspGI CCWGG 2 cut(s) 24, 921
PspN4I GGNNCC 4 cut(s) 395, 820, 1412, 1569
PspPI GGNCC 3 cut(s) 938, 1450, 1668
PsrI GAACNNNNNNTAC 2 cut(s) 747, 779
PstNI CAGNNNCTG 2 cut(s) 776, 1016
PsuI RGATCY 4 cut(s) 328, 818, 1073, 1873
PvuI CGATCG 1 cut(s) 355
PvuII CAGCTG 2 cut(s) 1434, 1665
RsaI GTAC 3 cut(s) 239, 278, 1680
RsaNI GTAC 3 cut(s) 238, 277, 1679
RseI CAYNNNNRTG 1 cut(s) 343
SacI GAGCTC 1 cut(s) 457
SatI GCNGC 8 cut(s) 654, 780, 838, 1435, 1476, 1574, 1929, 1938
Sau3AI GATC 7 cut(s) 34, 259, 328, 352, 818, 1073, 1873
Sau96I GGNCC 3 cut(s) 938, 1450, 1668
ScaI AGTACT 1 cut(s) 239
SchI GAGTC 1 cut(s) 995
ScrFI CCNGG 2 cut(s) 26, 923
SduI GDGCHC 1 cut(s) 457
SfaNI GCATC 3 cut(s) 471, 661, 1255
SfcI CTRYAG 2 cut(s) 1032, 1660
Sfr274I CTCGAG 1 cut(s) 456
SinI GGWCC 1 cut(s) 938
SlaI CTCGAG 1 cut(s) 456
SmiMI CAYNNNNRTG 1 cut(s) 343
SmlI CTYRAG 2 cut(s) 456, 1898
SmoI CTYRAG 2 cut(s) 456, 1898
SnaBI TACGTA 1 cut(s) 503
SseBI AGGCCT 2 cut(s) 799, 1030
SsiI CCGC 6 cut(s) 635, 837, 1400, 1571, 1928, 1931
SspI AATATT 1 cut(s) 1180
SspMI CTAG 5 cut(s) 243, 263, 975, 1407, 1877
SstI GAGCTC 1 cut(s) 457
StuI AGGCCT 2 cut(s) 799, 1030
StyD4I CCNGG 2 cut(s) 24, 921
StyI CCWWGG 1 cut(s) 1202
TaaI ACNGT 3 cut(s) 529, 536, 628
TaiI ACGT 3 cut(s) 505, 642, 762
TaqI TCGA 4 cut(s) 72, 135, 457, 1911
TaqII GACCGA 1 cut(s) 1109
TatI WGTACW 2 cut(s) 237, 1678
TauI GCSGC 2 cut(s) 840, 1931
TfiI GAWTC 2 cut(s) 102, 1521
TscAI CASTG 3 cut(s) 90, 711, 1692
TseFI GTSAC 2 cut(s) 443, 577
TseI GCWGC 6 cut(s) 653, 779, 1434, 1475, 1573, 1937
Tsp45I GTSAC 2 cut(s) 443, 577
TspDTI ATGAA 3 cut(s) 387, 820, 1010
TspGWI ACGGA 1 cut(s) 1372
TspRI CASTG 3 cut(s) 90, 711, 1692
VpaK11BI GGWCC 1 cut(s) 938
VspI ATTAAT 1 cut(s) 1802
XapI RAATTY 4 cut(s) 149, 171, 230, 943
XbaI TCTAGA 2 cut(s) 242, 262
XhoI CTCGAG 1 cut(s) 456
XmnI GAANNNNTTC 2 cut(s) 536, 759
XspI CTAG 5 cut(s) 243, 263, 975, 1407, 1877
ZrmI AGTACT 1 cut(s) 239
Zsp2I ATGCAT 1 cut(s) 1270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.