Rroxscaffold_1G00072300

Chaperone protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0008846
Physical Location & Seq
Forward (+)
93420752 .. 93425534
4783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00072300.1

Sequence Viewer

Length: 1797 bp
ATGAAGGTAGTAGAGCTTCTAGAGGAGGCTTGTTTAAGATTGAGGCATAAGTTTGACTACTCGAGAGAAGGAGAGGATGAACTAGAGGTGGGGGAGTACAGGTTGCATCGTGCACTTGTTGAGTTGAATGAAATTGAAAAAGAAAGAGAGCCATCCACCCAAAAATGGAGGGATCGTGTGGCCAAGGAACGTGAAGAACTAAACGATATGTGGAACAAGTTGGAGATAAAGTGGTTGGAATTACAAGCTTCACGGCAGAGATATGACATCAGAGCTCGGGAGGCACTTTTGTTGGAAAGGATGCGGGAAGGGCTGGTTTACTATTTAAATGAGGAAAGATCGAAGGAAAATTTCTCTGGGTTGTTGCAATGGCTTGATGAACATGTGACTGAGCTTTTATATACAACTCGCAAAGATTTTAAGCATGAGCTGACCGTTAGCTCTCATGCTATTGCACTGGCAGCAAGCTTGTTGATTGATGCTCCACCATCGCAGTTGCTTTCCGAATCACCAGTACTTCAGAACCATGAATCAATTAGGAGGGTAATTGGACAAGAACATGTTCTTCTTGCAGTCAGTGCTGCTTTATCGAGGCAAAGGCCTCCACGACGCCCTATTGGATCCTTCATCTTCATGTGTGGATCCGGTTGTGGGAGGACAGAAATTGCCAAACTTTTGGCCACTCTAGTCTTTGGTAAAAAAGACATGCTAACCGAGTTTGATCTTGAAAAGTACACAGGACCAAATTCTTTATCACGCCTTCTTGGTGTTCTCTGTAGCCACATTGAACCAGGCAAGAAGGGAGAGCTATGTGTTAAGAAGAGGCATCTAGGTGTCATTTTGTTTGACAATGTTGACAAGGCTCCTGCGCCTATTATTGAGCTTTTGACCCAGATTATAGGCTTTGGTTATCTAGTAGATGGTAATGGGAATAGTGTTGACTTCAATAATACGATGATCATCATGACGACAAATATTGGATGTGATCAATTGTGGCCGTGGAATTGCAAATGTGCTGATGAAGTTCAAAAGTTTCCTGTCAAAGAAGGATTATTTGATGATGCATGGGAAAGAAATCATAATTCCTGTTACCTATCGCTTCTGAGGGAGACAAAAAATCATTTTGGACCTCAATTTCTTGAATATGTAGATGATGTGATTGGATTTAGGTCTCTTTCCTCTCAACAATTGAAGGCTGTTGCTAGGCTCCAGCTAAGGGACCTTGCCGTGTACATGACCCAGAAGGGCCTCATCATTTACTCCTCTGAAGCTGCATTGGATATTATTGTGCGGAGATCAGCTTGGCCTGGAGATCGAATTAAAGGTGGAGAGAAAATAAGGATGTGGCTGGAACAGAACTTGGTCCCTGTGCTCTTCGAGATGATTTCAAAGAATGAGATATCTGATTTATCTATCATGTATATTGAAGCGTCAGTGGAGTCAGATCAATTGTCTTTCAGAATGGAAAAGACGGGACGTCTATTAGAAGAACGAGACGTTAATCAGTTAGCCTCTTTAAAGGAATTGAGATTAATGTACCAGAAAGAGAAAGAAAGAGCAAAGCGAATCTACATGCTGAGAAAATTTCAGAACAAATTGATGGCTAGTGCAAATGCTGGATTTGATCATGTGACGAATGTTGTTCAAGATCTGGTCAATATAATTCAGGATCTCATTACAATTCCTGGTAGCATCGTCAGGTCCTTCCTGGATAATCCTAAAAAGGTGGCAATGGCGGCCTTGAATGAGGATGAGGTTAGGCAGAACAAGAGGGCAAAAAAAGGGGCCAAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04213 map04213
Pfam Domains
Protein Families
AAA_2

Protein Analysis

598

Amino Acids

68.78

Weight (kDa)

6.89

Isoelectric Point (pI)

45.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_2 PF07724 204 - 381 2.6e-23 AAA domain (Cdc48 subfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1480
AciI CCGC 3 cut(s) 304, 1291, 1736
AclWI GGATC 6 cut(s) 180, 615, 628, 636, 649, 1677
AcoI YGGCCR 3 cut(s) 180, 678, 995
AcsI RAATTY 3 cut(s) 349, 745, 1583
AcuI CTGAAG 2 cut(s) 503, 1287
AcyI GRCGYC 2 cut(s) 610, 1477
AfaI GTAC 5 cut(s) 98, 516, 734, 1232, 1538
AfiI CCNNNNNNNGG 3 cut(s) 165, 651, 1216
AflIII ACRYGT 2 cut(s) 382, 559
AhdI GACNNNNNGTC 1 cut(s) 1476
AjnI CCWGG 4 cut(s) 790, 1306, 1684, 1707
Alw21I GWGCWC 3 cut(s) 115, 277, 1374
Alw26I GTCTC 3 cut(s) 1103, 1176, 1488
Alw44I GTGCAC 1 cut(s) 111
AlwI GGATC 6 cut(s) 180, 615, 628, 636, 649, 1677
Ama87I CYCGRG 2 cut(s) 61, 276
AoxI GGCC 8 cut(s) 180, 599, 678, 995, 1246, 1304, 1737, 1785
ApaLI GTGCAC 1 cut(s) 111
ApeKI GCWGC 3 cut(s) 461, 581, 1271
ApoI RAATTY 3 cut(s) 349, 745, 1583
AseI ATTAAT 1 cut(s) 1532
Asp700I GAANNNNTTC 1 cut(s) 561
AspLEI GCGC 1 cut(s) 871
AspS9I GGNCC 7 cut(s) 740, 1127, 1219, 1246, 1363, 1701, 1785
AsuHPI GGTGA 1 cut(s) 501
AvaI CYCGRG 2 cut(s) 61, 276
AvaII GGWCC 5 cut(s) 740, 1127, 1219, 1363, 1701
BaeGI GKGCMC 1 cut(s) 115
BalI TGGCCA 2 cut(s) 182, 680
BamHI GGATCC 2 cut(s) 620, 641
BanII GRGCYC 1 cut(s) 277
BarI GAAGNNNNNNTAC 1 cut(s) 32
Bbv12I GWGCWC 3 cut(s) 115, 277, 1374
BbvI GCAGC 3 cut(s) 473, 568, 1258
BccI CCATC 4 cut(s) 160, 496, 914, 1594
BceAI ACGGC 3 cut(s) 269, 982, 1211
BciT130I CCWGG 4 cut(s) 792, 1308, 1686, 1709
BclI TGATCA 3 cut(s) 957, 985, 1624
BcoDI GTCTC 3 cut(s) 1103, 1176, 1488
BfaI CTAG 7 cut(s) 20, 83, 686, 830, 914, 1203, 1605
BfmI CTRYAG 1 cut(s) 775
BglII AGATCT 1 cut(s) 1648
BisI GCNGC 4 cut(s) 462, 582, 1272, 1737
BlsI GCNGC 4 cut(s) 463, 583, 1273, 1738
BmcAI AGTACT 1 cut(s) 516
Bme1390I CCNGG 4 cut(s) 792, 1308, 1686, 1709
Bme18I GGWCC 5 cut(s) 740, 1127, 1219, 1363, 1701
BmeRI GACNNNNNGTC 1 cut(s) 1476
BmeT110I CYCGRG 2 cut(s) 61, 276
BmgT120I GGNCC 7 cut(s) 740, 1127, 1219, 1246, 1363, 1701, 1785
BmiI GGNNCC 7 cut(s) 622, 643, 864, 1208, 1220, 1365, 1786
BmrFI CCNGG 4 cut(s) 792, 1308, 1686, 1709
BmsI GCATC 6 cut(s) 115, 291, 469, 835, 1051, 1701
BpmI CTGGAG 2 cut(s) 1193, 1329
Bpu10I CCTNAGC 1 cut(s) 1214
BsaBI GATNNNNATC 1 cut(s) 959
BsaHI GRCGYC 2 cut(s) 610, 1477
BsaI GGTCTC 1 cut(s) 1176
BsaJI CCNNGG 3 cut(s) 183, 998, 1788
BsaWI WCCGGW 1 cut(s) 644
BsaXI ACNNNNNCTCC 6 cut(s) 86, 116, 160, 190, 215, 245
Bsc4I CCNNNNNNNGG 3 cut(s) 165, 651, 1216
Bse1I ACTGG 2 cut(s) 462, 512
Bse3DI GCAATG 2 cut(s) 374, 1737
Bse8I GATNNNNATC 1 cut(s) 959
BseBI CCWGG 4 cut(s) 792, 1308, 1686, 1709
BseDI CCNNGG 3 cut(s) 183, 998, 1788
BseGI GGATG 6 cut(s) 82, 152, 306, 986, 1347, 1756
BseJI GATNNNNATC 1 cut(s) 959
BseLI CCNNNNNNNGG 3 cut(s) 165, 651, 1216
BseMI GCAATG 2 cut(s) 374, 1737
BseMII CTCAG 3 cut(s) 381, 1094, 1568
BseNI ACTGG 2 cut(s) 462, 512
BseRI GAGGAG 2 cut(s) 38, 1252
BseSI GKGCMC 1 cut(s) 115
BseXI GCAGC 3 cut(s) 473, 568, 1258
BshFI GGCC 8 cut(s) 182, 601, 680, 997, 1248, 1306, 1739, 1787
BsiHKAI GWGCWC 3 cut(s) 115, 277, 1374
BsiHKCI CYCGRG 2 cut(s) 61, 276
BsiSI CCGG 1 cut(s) 645
BslFI GGGAC 3 cut(s) 1232, 1349, 1488
BslI CCNNNNNNNGG 3 cut(s) 165, 651, 1216
BsmAI GTCTC 3 cut(s) 1103, 1176, 1488
BsmBI CGTCTC 1 cut(s) 1488
BsmFI GGGAC 3 cut(s) 1232, 1349, 1488
BsnI GGCC 8 cut(s) 182, 601, 680, 997, 1248, 1306, 1739, 1787
Bso31I GGTCTC 1 cut(s) 1176
BsoBI CYCGRG 2 cut(s) 61, 276
Bsp1286I GDGCHC 3 cut(s) 115, 277, 1374
Bsp1407I TGTACA 1 cut(s) 1230
BspACI CCGC 3 cut(s) 304, 1291, 1736
BspANI GGCC 8 cut(s) 182, 601, 680, 997, 1248, 1306, 1739, 1787
BspCNI CTCAG 3 cut(s) 382, 1095, 1569
BspHI TCATGA 1 cut(s) 963
BspLI GGNNCC 7 cut(s) 622, 643, 864, 1208, 1220, 1365, 1786
BspPI GGATC 6 cut(s) 180, 615, 628, 636, 649, 1677
BspQI GCTCTTC 1 cut(s) 1379
BspTNI GGTCTC 1 cut(s) 1176
BsrDI GCAATG 2 cut(s) 374, 1737
BsrGI TGTACA 1 cut(s) 1230
BsrI ACTGG 2 cut(s) 462, 512
BssECI CCNNGG 3 cut(s) 183, 998, 1788
BssNI GRCGYC 2 cut(s) 610, 1477
BssT1I CCWWGG 2 cut(s) 183, 1788
Bst2UI CCWGG 4 cut(s) 792, 1308, 1686, 1709
Bst4CI ACNGT 1 cut(s) 436
Bst6I CTCTTC 2 cut(s) 815, 1379
BstACI GRCGYC 2 cut(s) 610, 1477
BstAPI GCANNNNNTGC 1 cut(s) 578
BstAUI TGTACA 1 cut(s) 1230
BstC8I GCNNGC 1 cut(s) 466
BstDEI CTNAG 4 cut(s) 390, 1103, 1214, 1577
BstDSI CCRYGG 1 cut(s) 998
BstF5I GGATG 6 cut(s) 82, 152, 306, 986, 1347, 1756
BstHHI GCGC 1 cut(s) 871
BstMAI GTCTC 3 cut(s) 1103, 1176, 1488
BstMWI GCNNNNNNNGC 5 cut(s) 281, 310, 461, 578, 1736
BstNI CCWGG 4 cut(s) 792, 1308, 1686, 1709
BstNSI RCATGY 4 cut(s) 386, 563, 709, 1576
BstSCI CCNGG 4 cut(s) 790, 1306, 1684, 1707
BstSFI CTRYAG 1 cut(s) 775
BstSLI GKGCMC 1 cut(s) 115
BstV1I GCAGC 3 cut(s) 473, 568, 1258
BstX2I RGATCY 4 cut(s) 620, 641, 1648, 1669
BstXI CCANNNNNNTGG 1 cut(s) 676
BstYI RGATCY 4 cut(s) 620, 641, 1648, 1669
BsuRI GGCC 8 cut(s) 182, 601, 680, 997, 1248, 1306, 1739, 1787
BtgI CCRYGG 1 cut(s) 998
BtgZI GCGATG 1 cut(s) 474
BtsCI GGATG 6 cut(s) 82, 152, 306, 986, 1347, 1756
BtsIMutI CAGTG 3 cut(s) 455, 583, 1440
Cac8I GCNNGC 1 cut(s) 466
CciI TCATGA 1 cut(s) 963
CfoI GCGC 1 cut(s) 871
Cfr13I GGNCC 7 cut(s) 740, 1127, 1219, 1246, 1363, 1701, 1785
CseI GACGC 2 cut(s) 618, 1419
Csp6I GTAC 5 cut(s) 97, 515, 733, 1231, 1537
CviQI GTAC 5 cut(s) 97, 515, 733, 1231, 1537
DdeI CTNAG 4 cut(s) 390, 1103, 1214, 1577
DraI TTTAAA 2 cut(s) 327, 1518
DriI GACNNNNNGTC 1 cut(s) 1476
EaeI YGGCCR 3 cut(s) 180, 678, 995
Eam1104I CTCTTC 2 cut(s) 815, 1379
Eam1105I GACNNNNNGTC 1 cut(s) 1476
EarI CTCTTC 2 cut(s) 815, 1379
Ecl136II GAGCTC 1 cut(s) 275
Eco130I CCWWGG 2 cut(s) 183, 1788
Eco147I AGGCCT 1 cut(s) 601
Eco24I GRGCYC 1 cut(s) 277
Eco31I GGTCTC 1 cut(s) 1176
Eco32I GATATC 1 cut(s) 1401
Eco47I GGWCC 5 cut(s) 740, 1127, 1219, 1363, 1701
Eco53kI GAGCTC 1 cut(s) 275
Eco57I CTGAAG 2 cut(s) 503, 1287
Eco88I CYCGRG 2 cut(s) 61, 276
EcoICRI GAGCTC 1 cut(s) 275
EcoO109I RGGNCCY 3 cut(s) 1219, 1246, 1701
EcoRII CCWGG 4 cut(s) 790, 1306, 1684, 1707
EcoRV GATATC 1 cut(s) 1401
EcoT14I CCWWGG 2 cut(s) 183, 1788
EcoT22I ATGCAT 1 cut(s) 1066
EcoT38I GRGCYC 1 cut(s) 277
ErhI CCWWGG 2 cut(s) 183, 1788
Esp3I CGTCTC 1 cut(s) 1488
FaqI GGGAC 3 cut(s) 1232, 1349, 1488
FauI CCCGC 1 cut(s) 297
FbaI TGATCA 3 cut(s) 957, 985, 1624
Fnu4HI GCNGC 4 cut(s) 462, 582, 1272, 1737
FokI GGATG 6 cut(s) 89, 139, 313, 993, 1354, 1763
FriOI GRGCYC 1 cut(s) 277
Fsp4HI GCNGC 4 cut(s) 462, 582, 1272, 1737
FspBI CTAG 7 cut(s) 20, 83, 686, 830, 914, 1203, 1605
GlaI GCGC 1 cut(s) 870
GluI GCNGC 4 cut(s) 462, 582, 1272, 1737
GsuI CTGGAG 2 cut(s) 1193, 1329
HaeIII GGCC 8 cut(s) 182, 601, 680, 997, 1248, 1306, 1739, 1787
HapII CCGG 1 cut(s) 645
HgaI GACGC 2 cut(s) 618, 1419
HhaI GCGC 1 cut(s) 871
Hin1I GRCGYC 2 cut(s) 610, 1477
Hin6I GCGC 1 cut(s) 869
HinP1I GCGC 1 cut(s) 869
HincII GTYRAC 2 cut(s) 856, 940
HindII GTYRAC 2 cut(s) 856, 940
HindIII AAGCTT 2 cut(s) 246, 466
HinfI GANTC 4 cut(s) 506, 530, 1439, 1566
HpaII CCGG 1 cut(s) 645
HphI GGTGA 1 cut(s) 501
Hpy166II GTNNAC 6 cut(s) 113, 319, 735, 856, 940, 1231
Hpy188I TCNGA 9 cut(s) 272, 505, 522, 1104, 1267, 1405, 1444, 1460, 1590
Hpy188III TCNNGA 9 cut(s) 20, 63, 278, 725, 964, 1139, 1378, 1646, 1667
Hpy8I GTNNAC 6 cut(s) 113, 319, 735, 856, 940, 1231
Hpy99I CGWCG 1 cut(s) 612
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4IV ACGT 3 cut(s) 190, 1477, 1497
HpyCH4V TGCA 9 cut(s) 106, 113, 367, 455, 572, 1008, 1064, 1274, 1610
HpyF10VI GCNNNNNNNGC 5 cut(s) 281, 310, 461, 578, 1736
HpyF3I CTNAG 4 cut(s) 390, 1103, 1214, 1577
HpySE526I ACGT 3 cut(s) 190, 1477, 1497
Hsp92I GRCGYC 2 cut(s) 610, 1477
HspAI GCGC 1 cut(s) 869
Ksp22I TGATCA 3 cut(s) 957, 985, 1624
LguI GCTCTTC 1 cut(s) 1379
LmnI GCTCC 3 cut(s) 487, 868, 1212
Lsp1109I GCAGC 3 cut(s) 473, 568, 1258
LweI GCATC 6 cut(s) 115, 291, 469, 835, 1051, 1701
MaeI CTAG 7 cut(s) 20, 83, 686, 830, 914, 1203, 1605
MaeII ACGT 3 cut(s) 190, 1477, 1497
MaeIII GTNAC 3 cut(s) 385, 1088, 1630
MboII GAAGA 6 cut(s) 206, 557, 622, 832, 1366, 1499
MfeI CAATTG 3 cut(s) 989, 1187, 1448
MflI RGATCY 4 cut(s) 620, 641, 1648, 1669
MhlI GDGCHC 3 cut(s) 115, 277, 1374
MlsI TGGCCA 2 cut(s) 182, 680
MluNI TGGCCA 2 cut(s) 182, 680
MlyI GAGTC 1 cut(s) 1448
MmeI TCCRAC 3 cut(s) 201, 216, 273
Mox20I TGGCCA 2 cut(s) 182, 680
Mph1103I ATGCAT 1 cut(s) 1066
MroXI GAANNNNTTC 1 cut(s) 561
MscI TGGCCA 2 cut(s) 182, 680
MseI TTAA 8 cut(s) 35, 326, 420, 816, 1320, 1500, 1517, 1532
MslI CAYNNNNRTG 2 cut(s) 632, 831
Msp20I TGGCCA 2 cut(s) 182, 680
MspI CCGG 1 cut(s) 645
MspR9I CCNGG 4 cut(s) 792, 1308, 1686, 1709
MunI CAATTG 3 cut(s) 989, 1187, 1448
MvaI CCWGG 4 cut(s) 792, 1308, 1686, 1709
MwoI GCNNNNNNNGC 5 cut(s) 281, 310, 461, 578, 1736
NlaIV GGNNCC 7 cut(s) 622, 643, 864, 1208, 1220, 1365, 1786
NmuCI GTSAC 2 cut(s) 385, 1630
NsiI ATGCAT 1 cut(s) 1066
NspI RCATGY 4 cut(s) 386, 563, 709, 1576
PaeR7I CTCGAG 1 cut(s) 61
PagI TCATGA 1 cut(s) 963
PceI AGGCCT 1 cut(s) 601
PciI ACATGT 2 cut(s) 382, 559
PciSI GCTCTTC 1 cut(s) 1379
PdmI GAANNNNTTC 1 cut(s) 561
PfeI GAWTC 3 cut(s) 506, 530, 1566
PfoI TCCNGGA 1 cut(s) 1707
PkrI GCNGC 4 cut(s) 463, 583, 1273, 1738
PleI GAGTC 1 cut(s) 1447
PpsI GAGTC 1 cut(s) 1447
PpuMI RGGWCCY 2 cut(s) 1219, 1701
PscI ACATGT 2 cut(s) 382, 559
PshBI ATTAAT 1 cut(s) 1532
Psp124BI GAGCTC 1 cut(s) 277
Psp5II RGGWCCY 2 cut(s) 1219, 1701
Psp6I CCWGG 4 cut(s) 790, 1306, 1684, 1707
PspGI CCWGG 4 cut(s) 790, 1306, 1684, 1707
PspN4I GGNNCC 7 cut(s) 622, 643, 864, 1208, 1220, 1365, 1786
PspPI GGNCC 7 cut(s) 740, 1127, 1219, 1246, 1363, 1701, 1785
PspPPI RGGWCCY 2 cut(s) 1219, 1701
PsuI RGATCY 4 cut(s) 620, 641, 1648, 1669
RsaI GTAC 5 cut(s) 98, 516, 734, 1232, 1538
RsaNI GTAC 5 cut(s) 97, 515, 733, 1231, 1537
RseI CAYNNNNRTG 2 cut(s) 632, 831
SacI GAGCTC 1 cut(s) 277
SapI GCTCTTC 1 cut(s) 1379
SaqAI TTAA 8 cut(s) 35, 326, 420, 816, 1320, 1500, 1517, 1532
SatI GCNGC 4 cut(s) 462, 582, 1272, 1737
Sau96I GGNCC 7 cut(s) 740, 1127, 1219, 1246, 1363, 1701, 1785
ScaI AGTACT 1 cut(s) 516
SchI GAGTC 1 cut(s) 1448
ScrFI CCNGG 4 cut(s) 792, 1308, 1686, 1709
SduI GDGCHC 3 cut(s) 115, 277, 1374
SfaNI GCATC 6 cut(s) 115, 291, 469, 835, 1051, 1701
SfcI CTRYAG 1 cut(s) 775
Sfr274I CTCGAG 1 cut(s) 61
SinI GGWCC 5 cut(s) 740, 1127, 1219, 1363, 1701
SlaI CTCGAG 1 cut(s) 61
SmiI ATTTAAAT 1 cut(s) 327
SmiMI CAYNNNNRTG 2 cut(s) 632, 831
SmlI CTYRAG 1 cut(s) 61
SmoI CTYRAG 1 cut(s) 61
SseBI AGGCCT 1 cut(s) 601
SsiI CCGC 3 cut(s) 304, 1291, 1736
SspI AATATT 1 cut(s) 976
SspMI CTAG 7 cut(s) 20, 83, 686, 830, 914, 1203, 1605
SstI GAGCTC 1 cut(s) 277
StuI AGGCCT 1 cut(s) 601
StyD4I CCNGG 4 cut(s) 790, 1306, 1684, 1707
StyI CCWWGG 2 cut(s) 183, 1788
SwaI ATTTAAAT 1 cut(s) 327
TaaI ACNGT 1 cut(s) 436
TaiI ACGT 3 cut(s) 193, 1480, 1500
TaqI TCGA 5 cut(s) 62, 341, 590, 1315, 1377
TatI WGTACW 4 cut(s) 96, 514, 732, 1230
TauI GCSGC 1 cut(s) 1739
TfiI GAWTC 3 cut(s) 506, 530, 1566
Tru1I TTAA 8 cut(s) 35, 326, 420, 816, 1320, 1500, 1517, 1532
Tru9I TTAA 8 cut(s) 35, 326, 420, 816, 1320, 1500, 1517, 1532
TscAI CASTG 3 cut(s) 462, 583, 1440
TseFI GTSAC 2 cut(s) 385, 1630
TseI GCWGC 3 cut(s) 461, 581, 1271
Tsp45I GTSAC 2 cut(s) 385, 1630
TspDTI ATGAA 8 cut(s) 17, 93, 144, 393, 543, 616, 622, 1035
TspRI CASTG 3 cut(s) 462, 583, 1440
VneI GTGCAC 1 cut(s) 111
VpaK11BI GGWCC 5 cut(s) 740, 1127, 1219, 1363, 1701
VspI ATTAAT 1 cut(s) 1532
XapI RAATTY 3 cut(s) 349, 745, 1583
XbaI TCTAGA 1 cut(s) 19
XceI RCATGY 4 cut(s) 386, 563, 709, 1576
XhoI CTCGAG 1 cut(s) 61
XmnI GAANNNNTTC 1 cut(s) 561
XspI CTAG 7 cut(s) 20, 83, 686, 830, 914, 1203, 1605
ZraI GACGTC 1 cut(s) 1478
ZrmI AGTACT 1 cut(s) 516
Zsp2I ATGCAT 1 cut(s) 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.