MD10G1318200.v1.1

Chaperone protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008846
Physical Location & Seq
Forward (+)
40103488 .. 40106514
3027 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1318200.v1.1.491

Sequence Viewer

Length: 1536 bp
ATGGGGGATTTTGTTAAGAAGCTCCAGGTTGAAGATCAGGAGAAGTTGGAGCTGGCTTATGGTGTTGAAATCGAAGCGGAGGCAGTGTTAGTTGCTTCCCGCATCATCATGCAATACCCCGATGTTTTTCAGTTCAATAGTAGTGGTGGTGAATTCGATGACACCTGGAAGGAAATTTTGCAAAATAAGGTTGTTGAGCTTTTAGAGGAAGCTTGTGTAAAATTGAGGCATAGATTTGAGTATTCTAGACAAGAAGAGGATGATCTAGATGCCAGTGAGTACCAGTTGCGTCGCACACTAGTTGAGTTAAACGAAATCAAGAAAGAAAGAGATCCATCCACACAAAAGTGGAGCGATCGTTTGACAAAGGAACATGAAGAATTGAAGGTTATGTGGAACCAGTTGGAGATGAACTGGATGCAAGTAAAAACTTCACGGCAGAGATATGACATCAGAGCTCGAGAGGCGCTTTTGTTGGAAAGGATGCATGAAAGGTTGGTCTTTGGTATAAATGAGGCAAGACTAAATATGGTGAACAGTTTCTCTGGGATGTTGCAATGGATTGATGACCATGTGACTGAGCTTTTATATACAACTCGCAAAGATTTTAAGCATGAGCTAACCGTTAGCCCACACATTGTTGCGAAGGCGGCAAGCCCCTTGATTGATGCTCCACCACCATTGTTGCTTTCTGAACCTACACTGCCTAAGAACCTTGAACAAAGAATTGCTAAGAGGGTAATTGGGCAAGAACATGTTCTACTTGCAATAACTGCTGCTCTATCAAAGCAGAGGCCTGAGCGACGCCCTATTGGGTCCTTCCTCTTCATGTGCGGCTCCGGTTATGGGAGGACAGAAATGGCCAAATCTTTAGCCGGACTACTTTTTGATAACAAAGACATGATAGCAGAGTTTGACCTGGCAAAATACTCAGGTCCAAATTCTTTCTCACGCCTTGTTGGACTTCTTTCTAGTCATGTGGAGCCGGGCATGAAGAGGGAGCGCTCAGAGGCTGTTAAGAAGAGGCCTATAGGTGTCATTTTGTTTGACAATGTTGACAAGGCTCATGAATCTGTTATTGACATTCTGACCGAGATTATAGGTTTTGGTCATCTAATAGATGGTCAGGGGAACATTGTTGACTTCACTAAAACATTGATAATCATGACAACGAATGTTGGACGTGATAAATACTGGCCATGGAATTGCAAATGTGCAGATGAAGTTCAGAAGTTCCCCATCAAAGAAGGATTATATGATGATGAATGGGAAACAAAACACAATTTATGTTACCTTTCTCTTCTGAGGGAGGCAAAACAGCATTTCAGACCTCAGTTGCTTGAATATGTGGATGATGTGATTGGAATTAGGTCACTATCTGTTCAGCAGTTGAAGGCGGTTGCTAGACTGCAGCTAAGGGACATTGCCAGCTGCATGACGCAGAAGGGCCTCATTATATACCCATCTGAAGCTGCATTAGATATTATTGTCCAGAGGTCAACTTGGCTTGGAGATAGAATCGTATGTTTTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04213 map04213
Pfam Domains
Protein Families
AAA_2

Protein Analysis

512

Amino Acids

58.93

Weight (kDa)

5.78

Isoelectric Point (pI)

42.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_2 PF07724 269 - 448 8.3e-26 AAA domain (Cdc48 subfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 77, 100, 650, 834, 1397
AclWI GGATC 1 cut(s) 326
AcoI YGGCCR 2 cut(s) 861, 1196
AcsI RAATTY 3 cut(s) 152, 174, 940
AcuI CTGAAG 1 cut(s) 1488
AcyI GRCGYC 1 cut(s) 805
AfaI GTAC 1 cut(s) 281
AfeI AGCGCT 1 cut(s) 1004
AfiI CCNNNNNNNGG 1 cut(s) 846
AflIII ACRYGT 1 cut(s) 754
AgsI TTSAA 7 cut(s) 32, 68, 136, 385, 719, 1343, 1393
AhlI ACTAGT 1 cut(s) 298
AjiI CACGTC 1 cut(s) 1184
AjnI CCWGG 3 cut(s) 24, 164, 918
AleI CACNNNNGTG 1 cut(s) 346
Alw21I GWGCWC 1 cut(s) 460
AlwI GGATC 1 cut(s) 326
AlwNI CAGNNNCTG 1 cut(s) 1013
Ama87I CYCGRG 1 cut(s) 459
Aor51HI AGCGCT 1 cut(s) 1004
AoxI GGCC 5 cut(s) 794, 861, 1025, 1196, 1447
ApeKI GCWGC 4 cut(s) 776, 1411, 1431, 1472
ApoI RAATTY 3 cut(s) 152, 174, 940
Asp700I GAANNNNTTC 2 cut(s) 539, 756
AspLEI GCGC 2 cut(s) 469, 1005
AspS9I GGNCC 3 cut(s) 816, 935, 1447
AsuC2I CCSGG 1 cut(s) 987
AsuHPI GGTGA 2 cut(s) 161, 544
AvaI CYCGRG 1 cut(s) 459
AvaII GGWCC 2 cut(s) 816, 935
BalI TGGCCA 2 cut(s) 863, 1198
BanII GRGCYC 1 cut(s) 460
Bbv12I GWGCWC 1 cut(s) 460
BbvI GCAGC 4 cut(s) 763, 1418, 1423, 1459
BccI CCATC 4 cut(s) 343, 1115, 1247, 1471
BceAI ACGGC 1 cut(s) 452
BciT130I CCWGG 3 cut(s) 26, 166, 920
BcnI CCSGG 1 cut(s) 987
BcuI ACTAGT 1 cut(s) 298
BfaI CTAG 5 cut(s) 246, 266, 299, 972, 1404
BfmI CTRYAG 2 cut(s) 1029, 1409
BfoI RGCGCY 2 cut(s) 470, 1006
BisI GCNGC 6 cut(s) 651, 777, 835, 1412, 1432, 1473
BlsI GCNGC 6 cut(s) 652, 778, 836, 1413, 1433, 1474
Bme1390I CCNGG 4 cut(s) 26, 166, 920, 987
Bme18I GGWCC 2 cut(s) 816, 935
BmeT110I CYCGRG 1 cut(s) 459
BmgBI CACGTC 1 cut(s) 1184
BmgT120I GGNCC 3 cut(s) 816, 935, 1447
BmiI GGNNCC 4 cut(s) 398, 817, 838, 984
BmrFI CCNGG 4 cut(s) 26, 166, 920, 987
BmsI GCATC 5 cut(s) 111, 259, 408, 474, 658
BpmI CTGGAG 1 cut(s) 8
Bpu10I CCTNAGC 2 cut(s) 798, 1415
BpuMI CCSGG 1 cut(s) 987
BsaHI GRCGYC 1 cut(s) 805
BsaJI CCNNGG 1 cut(s) 1199
BsaWI WCCGGW 1 cut(s) 839
BsaXI ACNNNNNCTCC 4 cut(s) 71, 101, 343, 373
Bsc4I CCNNNNNNNGG 1 cut(s) 846
Bse1I ACTGG 5 cut(s) 273, 283, 400, 419, 1199
Bse3DI GCAATG 2 cut(s) 563, 1422
BseBI CCWGG 3 cut(s) 26, 166, 920
BseDI CCNNGG 1 cut(s) 1199
BseGI GGATG 6 cut(s) 265, 335, 423, 489, 555, 1357
BseLI CCNNNNNNNGG 1 cut(s) 846
BseMI GCAATG 2 cut(s) 563, 1422
BseMII CTCAG 6 cut(s) 570, 789, 945, 1020, 1295, 1346
BseNI ACTGG 5 cut(s) 273, 283, 400, 419, 1199
BseXI GCAGC 4 cut(s) 763, 1418, 1423, 1459
BsgI GTGCAG 1 cut(s) 1236
Bsh1285I CGRYCG 1 cut(s) 358
BshFI GGCC 5 cut(s) 796, 863, 1027, 1198, 1449
BsiEI CGRYCG 1 cut(s) 358
BsiHKAI GWGCWC 1 cut(s) 460
BsiHKCI CYCGRG 1 cut(s) 459
BsiSI CCGG 3 cut(s) 840, 876, 986
BslFI GGGAC 1 cut(s) 1433
BslI CCNNNNNNNGG 1 cut(s) 846
BsmFI GGGAC 1 cut(s) 1433
BsnI GGCC 5 cut(s) 796, 863, 1027, 1198, 1449
BsoBI CYCGRG 1 cut(s) 459
Bsp1286I GDGCHC 1 cut(s) 460
Bsp143I GATC 4 cut(s) 34, 262, 331, 355
Bsp19I CCATGG 1 cut(s) 1199
BspACI CCGC 5 cut(s) 77, 100, 650, 834, 1397
BspANI GGCC 5 cut(s) 796, 863, 1027, 1198, 1449
BspCNI CTCAG 6 cut(s) 571, 790, 944, 1019, 1296, 1345
BspHI TCATGA 2 cut(s) 1066, 1164
BspLI GGNNCC 4 cut(s) 398, 817, 838, 984
BspMAI CTGCAG 1 cut(s) 1413
BspPI GGATC 1 cut(s) 326
BsrDI GCAATG 2 cut(s) 563, 1422
BsrI ACTGG 5 cut(s) 273, 283, 400, 419, 1199
BssECI CCNNGG 1 cut(s) 1199
BssMI GATC 4 cut(s) 34, 262, 331, 355
BssNI GRCGYC 1 cut(s) 805
BssT1I CCWWGG 1 cut(s) 1199
Bst2UI CCWGG 3 cut(s) 26, 166, 920
Bst4CI ACNGT 2 cut(s) 539, 625
Bst6I CTCTTC 5 cut(s) 249, 830, 989, 1016, 1305
BstACI GRCGYC 1 cut(s) 805
BstAPI GCANNNNNTGC 1 cut(s) 773
BstC8I GCNNGC 3 cut(s) 54, 655, 1429
BstDEI CTNAG 9 cut(s) 579, 708, 732, 798, 931, 1006, 1304, 1332, 1415
BstDSI CCRYGG 1 cut(s) 1199
BstF5I GGATG 6 cut(s) 265, 335, 423, 489, 555, 1357
BstH2I RGCGCY 2 cut(s) 470, 1006
BstHHI GCGC 2 cut(s) 469, 1005
BstKTI GATC 4 cut(s) 37, 265, 334, 358
BstMBI GATC 4 cut(s) 34, 262, 331, 355
BstMCI CGRYCG 1 cut(s) 358
BstMWI GCNNNNNNNGC 3 cut(s) 464, 650, 773
BstNI CCWGG 3 cut(s) 26, 166, 920
BstNSI RCATGY 1 cut(s) 758
BstSCI CCNGG 4 cut(s) 24, 164, 918, 985
BstSFI CTRYAG 2 cut(s) 1029, 1409
BstV1I GCAGC 4 cut(s) 763, 1418, 1423, 1459
BstX2I RGATCY 1 cut(s) 331
BstYI RGATCY 1 cut(s) 331
BsuRI GGCC 5 cut(s) 796, 863, 1027, 1198, 1449
BtgI CCRYGG 1 cut(s) 1199
BtrI CACGTC 1 cut(s) 1184
BtsCI GGATG 6 cut(s) 265, 335, 423, 489, 555, 1357
BtsI GCAGTG 2 cut(s) 90, 701
BtsIMutI CAGTG 3 cut(s) 90, 280, 701
Cac8I GCNNGC 3 cut(s) 54, 655, 1429
CaiI CAGNNNCTG 1 cut(s) 1013
CciI TCATGA 2 cut(s) 1066, 1164
CfoI GCGC 2 cut(s) 469, 1005
Cfr13I GGNCC 3 cut(s) 816, 935, 1447
CseI GACGC 3 cut(s) 278, 813, 1447
Csp6I GTAC 1 cut(s) 280
CspCI CAANNNNNGTGG 6 cut(s) 124, 159, 663, 666, 698, 701
CviQI GTAC 1 cut(s) 280
DdeI CTNAG 9 cut(s) 579, 708, 732, 798, 931, 1006, 1304, 1332, 1415
DpnI GATC 4 cut(s) 36, 264, 333, 357
DpnII GATC 4 cut(s) 34, 262, 331, 355
EaeI YGGCCR 2 cut(s) 861, 1196
Eam1104I CTCTTC 5 cut(s) 249, 830, 989, 1016, 1305
EarI CTCTTC 5 cut(s) 249, 830, 989, 1016, 1305
Ecl136II GAGCTC 1 cut(s) 458
Eco130I CCWWGG 1 cut(s) 1199
Eco147I AGGCCT 2 cut(s) 796, 1027
Eco24I GRGCYC 1 cut(s) 460
Eco47I GGWCC 2 cut(s) 816, 935
Eco47III AGCGCT 1 cut(s) 1004
Eco53kI GAGCTC 1 cut(s) 458
Eco57I CTGAAG 1 cut(s) 1488
Eco88I CYCGRG 1 cut(s) 459
EcoICRI GAGCTC 1 cut(s) 458
EcoO109I RGGNCCY 2 cut(s) 816, 1447
EcoRI GAATTC 1 cut(s) 152
EcoRII CCWGG 3 cut(s) 24, 164, 918
EcoT14I CCWWGG 1 cut(s) 1199
EcoT22I ATGCAT 1 cut(s) 489
EcoT38I GRGCYC 1 cut(s) 460
ErhI CCWWGG 1 cut(s) 1199
FaqI GGGAC 1 cut(s) 1433
FauI CCCGC 1 cut(s) 107
Fnu4HI GCNGC 6 cut(s) 651, 777, 835, 1412, 1432, 1473
FokI GGATG 6 cut(s) 272, 322, 430, 496, 562, 1364
FriOI GRGCYC 1 cut(s) 460
Fsp4HI GCNGC 6 cut(s) 651, 777, 835, 1412, 1432, 1473
FspBI CTAG 5 cut(s) 246, 266, 299, 972, 1404
GlaI GCGC 2 cut(s) 468, 1004
GluI GCNGC 6 cut(s) 651, 777, 835, 1412, 1432, 1473
GsuI CTGGAG 1 cut(s) 8
HaeII RGCGCY 2 cut(s) 470, 1006
HaeIII GGCC 5 cut(s) 796, 863, 1027, 1198, 1449
HapII CCGG 3 cut(s) 840, 876, 986
HgaI GACGC 3 cut(s) 278, 813, 1447
HhaI GCGC 2 cut(s) 469, 1005
Hin1I GRCGYC 1 cut(s) 805
Hin6I GCGC 2 cut(s) 467, 1003
HinP1I GCGC 2 cut(s) 467, 1003
HincII GTYRAC 3 cut(s) 1057, 1141, 1500
HindII GTYRAC 3 cut(s) 1057, 1141, 1500
HindIII AAGCTT 1 cut(s) 210
HinfI GANTC 2 cut(s) 1070, 1518
HpaII CCGG 3 cut(s) 840, 876, 986
HphI GGTGA 2 cut(s) 161, 544
Hpy166II GTNNAC 4 cut(s) 535, 1057, 1141, 1500
Hpy188I TCNGA 8 cut(s) 455, 694, 1009, 1089, 1230, 1305, 1328, 1468
Hpy188III TCNNGA 8 cut(s) 38, 246, 266, 319, 461, 1067, 1165, 1492
Hpy8I GTNNAC 4 cut(s) 535, 1057, 1141, 1500
Hpy99I CGWCG 2 cut(s) 294, 807
HpyAV CCTTC 7 cut(s) 163, 379, 640, 829, 1241, 1387, 1438
HpyCH4III ACNGT 2 cut(s) 539, 625
HpyCH4IV ACGT 1 cut(s) 1183
HpyF10VI GCNNNNNNNGC 3 cut(s) 464, 650, 773
HpyF3I CTNAG 9 cut(s) 579, 708, 732, 798, 931, 1006, 1304, 1332, 1415
HpySE526I ACGT 1 cut(s) 1183
Hsp92I GRCGYC 1 cut(s) 805
HspAI GCGC 2 cut(s) 467, 1003
Kzo9I GATC 4 cut(s) 34, 262, 331, 355
LmnI GCTCC 7 cut(s) 27, 49, 351, 676, 842, 982, 1000
Lsp1109I GCAGC 4 cut(s) 763, 1418, 1423, 1459
LweI GCATC 5 cut(s) 111, 259, 408, 474, 658
MaeI CTAG 5 cut(s) 246, 266, 299, 972, 1404
MaeII ACGT 1 cut(s) 1183
MaeIII GTNAC 3 cut(s) 574, 1289, 1371
MalI GATC 4 cut(s) 36, 264, 333, 357
MboI GATC 4 cut(s) 34, 262, 331, 355
MboII GAAGA 7 cut(s) 44, 266, 389, 817, 1006, 1033, 1292
MflI RGATCY 1 cut(s) 331
MhlI GDGCHC 1 cut(s) 460
MlsI TGGCCA 2 cut(s) 863, 1198
MluNI TGGCCA 2 cut(s) 863, 1198
MmeI TCCRAC 5 cut(s) 27, 384, 456, 940, 1159
Mox20I TGGCCA 2 cut(s) 863, 1198
Mph1103I ATGCAT 1 cut(s) 489
MroXI GAANNNNTTC 2 cut(s) 539, 756
MscI TGGCCA 2 cut(s) 863, 1198
MseI TTAA 4 cut(s) 15, 308, 609, 1017
MslI CAYNNNNRTG 2 cut(s) 107, 346
Msp20I TGGCCA 2 cut(s) 863, 1198
MspA1I CMGCKG 1 cut(s) 1431
MspI CCGG 3 cut(s) 840, 876, 986
MspR9I CCNGG 4 cut(s) 26, 166, 920, 987
MvaI CCWGG 3 cut(s) 26, 166, 920
MwoI GCNNNNNNNGC 3 cut(s) 464, 650, 773
NciI CCSGG 1 cut(s) 987
NcoI CCATGG 1 cut(s) 1199
NdeII GATC 4 cut(s) 34, 262, 331, 355
NlaIV GGNNCC 4 cut(s) 398, 817, 838, 984
NmuCI GTSAC 2 cut(s) 574, 1371
NsiI ATGCAT 1 cut(s) 489
NspI RCATGY 1 cut(s) 758
OliI CACNNNNGTG 1 cut(s) 346
PaeR7I CTCGAG 1 cut(s) 459
PagI TCATGA 2 cut(s) 1066, 1164
PceI AGGCCT 2 cut(s) 796, 1027
PciI ACATGT 1 cut(s) 754
PdmI GAANNNNTTC 2 cut(s) 539, 756
PfeI GAWTC 2 cut(s) 1070, 1518
PkrI GCNGC 6 cut(s) 652, 778, 836, 1413, 1433, 1474
Ple19I CGATCG 1 cut(s) 358
PpuMI RGGWCCY 1 cut(s) 816
PscI ACATGT 1 cut(s) 754
Psp124BI GAGCTC 1 cut(s) 460
Psp5II RGGWCCY 1 cut(s) 816
Psp6I CCWGG 3 cut(s) 24, 164, 918
PspGI CCWGG 3 cut(s) 24, 164, 918
PspN4I GGNNCC 4 cut(s) 398, 817, 838, 984
PspPI GGNCC 3 cut(s) 816, 935, 1447
PspPPI RGGWCCY 1 cut(s) 816
PsrI GAACNNNNNNTAC 2 cut(s) 744, 776
PstI CTGCAG 1 cut(s) 1413
PstNI CAGNNNCTG 1 cut(s) 1013
PsuI RGATCY 1 cut(s) 331
PvuI CGATCG 1 cut(s) 358
PvuII CAGCTG 1 cut(s) 1431
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
RseI CAYNNNNRTG 2 cut(s) 107, 346
SacI GAGCTC 1 cut(s) 460
SaqAI TTAA 4 cut(s) 15, 308, 609, 1017
SatI GCNGC 6 cut(s) 651, 777, 835, 1412, 1432, 1473
Sau3AI GATC 4 cut(s) 34, 262, 331, 355
Sau96I GGNCC 3 cut(s) 816, 935, 1447
ScrFI CCNGG 4 cut(s) 26, 166, 920, 987
SduI GDGCHC 1 cut(s) 460
SfaNI GCATC 5 cut(s) 111, 259, 408, 474, 658
SfcI CTRYAG 2 cut(s) 1029, 1409
Sfr274I CTCGAG 1 cut(s) 459
SinI GGWCC 2 cut(s) 816, 935
SlaI CTCGAG 1 cut(s) 459
SmiMI CAYNNNNRTG 2 cut(s) 107, 346
SmlI CTYRAG 1 cut(s) 459
SmoI CTYRAG 1 cut(s) 459
SpeI ACTAGT 1 cut(s) 298
SseBI AGGCCT 2 cut(s) 796, 1027
SsiI CCGC 5 cut(s) 77, 100, 650, 834, 1397
SspMI CTAG 5 cut(s) 246, 266, 299, 972, 1404
SstI GAGCTC 1 cut(s) 460
StuI AGGCCT 2 cut(s) 796, 1027
StyD4I CCNGG 4 cut(s) 24, 164, 918, 985
StyI CCWWGG 1 cut(s) 1199
TaaI ACNGT 2 cut(s) 539, 625
TaiI ACGT 1 cut(s) 1186
TaqI TCGA 3 cut(s) 72, 156, 460
TaqII GACCGA 1 cut(s) 1106
TauI GCSGC 2 cut(s) 653, 837
TfiI GAWTC 2 cut(s) 1070, 1518
Tru1I TTAA 4 cut(s) 15, 308, 609, 1017
Tru9I TTAA 4 cut(s) 15, 308, 609, 1017
TscAI CASTG 3 cut(s) 90, 280, 708
TseFI GTSAC 2 cut(s) 574, 1371
TseI GCWGC 4 cut(s) 776, 1411, 1431, 1472
Tsp45I GTSAC 2 cut(s) 574, 1371
TspDTI ATGAA 9 cut(s) 390, 425, 504, 817, 1007, 1083, 1236, 1278, 1521
TspRI CASTG 3 cut(s) 90, 280, 708
VpaK11BI GGWCC 2 cut(s) 816, 935
XapI RAATTY 3 cut(s) 152, 174, 940
XbaI TCTAGA 2 cut(s) 245, 265
XceI RCATGY 1 cut(s) 758
XhoI CTCGAG 1 cut(s) 459
XmnI GAANNNNTTC 2 cut(s) 539, 756
XspI CTAG 5 cut(s) 246, 266, 299, 972, 1404
Zsp2I ATGCAT 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.