Rh5DG031700

Chaperone protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0008846
Physical Location & Seq
Reverse (-)
2236362 .. 2241195
4834 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG031700.1

Sequence Viewer

Length: 1545 bp
ATGACAGGGATTGATGCAGAGCTATGCATGGAGGAGTTTGTGGCGAAAATCGAAGTGGAGGATCAGGAGAAGTTGGAGCTGGAGTATGGTGTTGAAATCGAAGCAGAGGCCGTGGTGGATGCATGTCGAATCATCAAGCAGTACCCTGATGTTTTTCAGTTTAATTGTAGAGGAACACTGGATGATACCGAGAGGGCTATTTTGCAAATGAAGGTAGTAGAGCTTCTAGAGGAGGCTTGTTTAAGATTGAGGCATAAGTTTGACTACTCGAGAGAAGGAGAGGATGAACTAGAGGTGGGGGAGTACAGGTTGCATCGTGCACTTGTTGAGTTGAATGAAATCGAAAAAGAAAGAGAGCCATCCACCCAAAAATGGAGGGATCGTGTGGCCAAGGAACGTGAAGAACTAAACGAAATGTGGAACAAGTTGGAGATAAAGTGGTTGGAATTACAAGCTTCACGGCAGAGATATGACATCAGAGCTCGGGAGGCACTTTTGTTGGAAAGGATGCGGGAAGGGCTGGTTTACTATCTAAATGAGGAAAGATCGAAGGAAAATTTCTCTGGGTTGTTGCAATGGCTTGATGAACATGTGACTGAGCTTTTATATACAACTCGCAAAGATTTTAAGCATGAGCTGACCGTTAGCTCGCATGCTATTGCACTGGCAGCAAGCTTGTTGATTGATGCTCCACCATCGCAGTTGCTTTCCGAATCACCAGTACTTCAGAACCATGAATCAATTAGGAGGGTAATTGGACAAGAACATGTTCTACTTGCAGTCAGTGCTGCTTTATCGAGGCAGAGGCCTCCACGACGCCCTATTGGATCCTTCATCTTCATGTGTGGATCCGGTTGTGGGAGGACAGAAATTGCCAAACTTTTGGCCACTCTAGTCTTTGGTAAAAAAGACATGCTAACCGAGTTTGATCTAGAAAAGTACACAGGACCAAATTCTTTATCACGCCTTCTTGGTGTTCTCTGTAGTAGCCACATTGAACCAGGCAAGAAGGGAGAGCTATGTGTTAAGAAGAGGCATCTAGGTGTCATTTTGTTTGACAATGTTGACAAGGCTCCTACTCCTATTATTGAGCTTTTGACCCAGATTATAGGCTTTGGTTATCTAGTAGATGGTAATGGGAATAGTGTTGACTTCACTAATACGATGATCATCATGACGACAAATATTGGATGTGATCAATTGTGGCCGTGGAATTGCAAATGTGCTGATGAAGTTCAAAAGTTTCCTGTCAAAGAAGGATTATTTGATGATGCATGGGAAAGAAATCATAATTCCTGTTACCTATCGCTTCTGAGGGAGACAAAAAATCATTTTGGACCTCAATTTCTTGAATACATAGATGATGTGATTGGATTTAGGTCTCTTTCCTCTCAACAATTGAAGGCTGTTGCTAGGCTCCAGCTAAGGGACCTTGCTGTCTACATGACCCAGAAGGGCCTCATCATTTACTCCTCTGAAGCTGCATTGGATATTATTGTGCGGAGATCAGCTTGGCCTGGAGATCGAATTGTAAGTCTTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04213 map04213
Pfam Domains
Protein Families
AAA_2

Protein Analysis

514

Amino Acids

59.02

Weight (kDa)

5.27

Isoelectric Point (pI)

46.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_2 PF07724 273 - 454 2.7e-22 AAA domain (Cdc48 subfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1436
AccI GTMKAC 1 cut(s) 1440
AciI CCGC 2 cut(s) 511, 1501
AclWI GGATC 6 cut(s) 69, 387, 822, 835, 843, 856
AcoI YGGCCR 3 cut(s) 387, 885, 1205
AcsI RAATTY 2 cut(s) 556, 952
AcuI CTGAAG 2 cut(s) 710, 1497
AcyI GRCGYC 1 cut(s) 817
AfaI GTAC 4 cut(s) 143, 305, 723, 941
AfiI CCNNNNNNNGG 3 cut(s) 372, 858, 1426
AflIII ACRYGT 2 cut(s) 589, 766
AgsI TTSAA 6 cut(s) 95, 334, 998, 1238, 1352, 1402
AjnI CCWGG 2 cut(s) 1000, 1516
Alw21I GWGCWC 2 cut(s) 322, 484
Alw26I GTCTC 2 cut(s) 1313, 1386
Alw44I GTGCAC 1 cut(s) 318
AlwI GGATC 6 cut(s) 69, 387, 822, 835, 843, 856
Ama87I CYCGRG 2 cut(s) 268, 483
AoxI GGCC 7 cut(s) 108, 387, 806, 885, 1205, 1456, 1514
ApaLI GTGCAC 1 cut(s) 318
ApeKI GCWGC 3 cut(s) 668, 788, 1481
ApoI RAATTY 2 cut(s) 556, 952
ArsI GACNNNNNNTTYG 1 cut(s) 1519
Asp700I GAANNNNTTC 1 cut(s) 768
AspS9I GGNCC 4 cut(s) 947, 1337, 1429, 1456
AsuHPI GGTGA 1 cut(s) 708
AvaI CYCGRG 2 cut(s) 268, 483
AvaII GGWCC 3 cut(s) 947, 1337, 1429
BaeGI GKGCMC 1 cut(s) 322
BalI TGGCCA 2 cut(s) 389, 887
BamHI GGATCC 2 cut(s) 827, 848
BanII GRGCYC 1 cut(s) 484
BarI GAAGNNNNNNTAC 2 cut(s) 207, 239
Bbv12I GWGCWC 2 cut(s) 322, 484
BbvI GCAGC 3 cut(s) 680, 775, 1468
BccI CCATC 3 cut(s) 367, 703, 1124
BceAI ACGGC 3 cut(s) 95, 476, 1192
BciT130I CCWGG 2 cut(s) 1002, 1518
BclI TGATCA 2 cut(s) 1167, 1195
BcoDI GTCTC 2 cut(s) 1313, 1386
BfaI CTAG 7 cut(s) 227, 290, 893, 932, 1040, 1124, 1413
BfmI CTRYAG 1 cut(s) 982
BisI GCNGC 3 cut(s) 669, 789, 1482
BlsI GCNGC 3 cut(s) 670, 790, 1483
BmcAI AGTACT 1 cut(s) 723
Bme1390I CCNGG 2 cut(s) 1002, 1518
Bme18I GGWCC 3 cut(s) 947, 1337, 1429
BmeT110I CYCGRG 2 cut(s) 268, 483
BmgT120I GGNCC 4 cut(s) 947, 1337, 1429, 1456
BmiI GGNNCC 5 cut(s) 829, 850, 1074, 1418, 1430
BmrFI CCNGG 2 cut(s) 1002, 1518
BmsI GCATC 7 cut(s) 4, 109, 322, 498, 676, 1045, 1261
BpmI CTGGAG 3 cut(s) 101, 1403, 1539
Bpu10I CCTNAGC 1 cut(s) 1424
BsaBI GATNNNNATC 1 cut(s) 1169
BsaHI GRCGYC 1 cut(s) 817
BsaI GGTCTC 1 cut(s) 1386
BsaJI CCNNGG 3 cut(s) 111, 390, 1208
BsaWI WCCGGW 1 cut(s) 851
BsaXI ACNNNNNCTCC 8 cut(s) 68, 98, 293, 323, 367, 397, 422, 452
Bsc4I CCNNNNNNNGG 3 cut(s) 372, 858, 1426
Bse1I ACTGG 3 cut(s) 183, 669, 719
Bse3DI GCAATG 1 cut(s) 581
Bse8I GATNNNNATC 1 cut(s) 1169
BseBI CCWGG 2 cut(s) 1002, 1518
BseDI CCNNGG 3 cut(s) 111, 390, 1208
BseGI GGATG 6 cut(s) 124, 187, 289, 359, 513, 1196
BseJI GATNNNNATC 1 cut(s) 1169
BseLI CCNNNNNNNGG 3 cut(s) 372, 858, 1426
BseMI GCAATG 1 cut(s) 581
BseMII CTCAG 2 cut(s) 588, 1304
BseNI ACTGG 3 cut(s) 183, 669, 719
BseRI GAGGAG 3 cut(s) 47, 245, 1462
BseSI GKGCMC 1 cut(s) 322
BseXI GCAGC 3 cut(s) 680, 775, 1468
BshFI GGCC 7 cut(s) 110, 389, 808, 887, 1207, 1458, 1516
BsiHKAI GWGCWC 2 cut(s) 322, 484
BsiHKCI CYCGRG 2 cut(s) 268, 483
BsiSI CCGG 1 cut(s) 852
BslFI GGGAC 1 cut(s) 1442
BslI CCNNNNNNNGG 3 cut(s) 372, 858, 1426
BsmAI GTCTC 2 cut(s) 1313, 1386
BsmFI GGGAC 1 cut(s) 1442
BsnI GGCC 7 cut(s) 110, 389, 808, 887, 1207, 1458, 1516
Bso31I GGTCTC 1 cut(s) 1386
BsoBI CYCGRG 2 cut(s) 268, 483
Bsp1286I GDGCHC 2 cut(s) 322, 484
BspACI CCGC 2 cut(s) 511, 1501
BspANI GGCC 7 cut(s) 110, 389, 808, 887, 1207, 1458, 1516
BspCNI CTCAG 2 cut(s) 589, 1305
BspHI TCATGA 1 cut(s) 1173
BspLI GGNNCC 5 cut(s) 829, 850, 1074, 1418, 1430
BspPI GGATC 6 cut(s) 69, 387, 822, 835, 843, 856
BspTNI GGTCTC 1 cut(s) 1386
BsrDI GCAATG 1 cut(s) 581
BsrI ACTGG 3 cut(s) 183, 669, 719
BssECI CCNNGG 3 cut(s) 111, 390, 1208
BssNI GRCGYC 1 cut(s) 817
BssT1I CCWWGG 1 cut(s) 390
Bst2UI CCWGG 2 cut(s) 1002, 1518
Bst4CI ACNGT 1 cut(s) 643
Bst6I CTCTTC 1 cut(s) 1025
BstACI GRCGYC 1 cut(s) 817
BstAPI GCANNNNNTGC 1 cut(s) 785
BstC8I GCNNGC 3 cut(s) 650, 654, 673
BstDEI CTNAG 3 cut(s) 597, 1313, 1424
BstDSI CCRYGG 2 cut(s) 111, 1208
BstF5I GGATG 6 cut(s) 124, 187, 289, 359, 513, 1196
BstMAI GTCTC 2 cut(s) 1313, 1386
BstMWI GCNNNNNNNGC 4 cut(s) 488, 517, 668, 785
BstNI CCWGG 2 cut(s) 1002, 1518
BstNSI RCATGY 5 cut(s) 126, 593, 656, 770, 916
BstSCI CCNGG 2 cut(s) 1000, 1516
BstSFI CTRYAG 1 cut(s) 982
BstSLI GKGCMC 1 cut(s) 322
BstV1I GCAGC 3 cut(s) 680, 775, 1468
BstX2I RGATCY 2 cut(s) 827, 848
BstXI CCANNNNNNTGG 1 cut(s) 883
BstYI RGATCY 2 cut(s) 827, 848
BsuRI GGCC 7 cut(s) 110, 389, 808, 887, 1207, 1458, 1516
BtgI CCRYGG 2 cut(s) 111, 1208
BtgZI GCGATG 1 cut(s) 681
BtsCI GGATG 6 cut(s) 124, 187, 289, 359, 513, 1196
BtsIMutI CAGTG 3 cut(s) 176, 662, 790
Cac8I GCNNGC 3 cut(s) 650, 654, 673
CciI TCATGA 1 cut(s) 1173
Cfr13I GGNCC 4 cut(s) 947, 1337, 1429, 1456
CseI GACGC 1 cut(s) 825
Csp6I GTAC 4 cut(s) 142, 304, 722, 940
CviQI GTAC 4 cut(s) 142, 304, 722, 940
DdeI CTNAG 3 cut(s) 597, 1313, 1424
DrdI GACNNNNNNGTC 1 cut(s) 1436
DseDI GACNNNNNNGTC 1 cut(s) 1436
EaeI YGGCCR 3 cut(s) 387, 885, 1205
Eam1104I CTCTTC 1 cut(s) 1025
EarI CTCTTC 1 cut(s) 1025
Ecl136II GAGCTC 1 cut(s) 482
Eco130I CCWWGG 1 cut(s) 390
Eco147I AGGCCT 1 cut(s) 808
Eco24I GRGCYC 1 cut(s) 484
Eco31I GGTCTC 1 cut(s) 1386
Eco47I GGWCC 3 cut(s) 947, 1337, 1429
Eco53kI GAGCTC 1 cut(s) 482
Eco57I CTGAAG 2 cut(s) 710, 1497
Eco88I CYCGRG 2 cut(s) 268, 483
EcoICRI GAGCTC 1 cut(s) 482
EcoO109I RGGNCCY 2 cut(s) 1429, 1456
EcoRII CCWGG 2 cut(s) 1000, 1516
EcoT14I CCWWGG 1 cut(s) 390
EcoT22I ATGCAT 3 cut(s) 29, 124, 1276
EcoT38I GRGCYC 1 cut(s) 484
ErhI CCWWGG 1 cut(s) 390
FaqI GGGAC 1 cut(s) 1442
FauI CCCGC 1 cut(s) 504
FbaI TGATCA 2 cut(s) 1167, 1195
FblI GTMKAC 1 cut(s) 1440
Fnu4HI GCNGC 3 cut(s) 669, 789, 1482
FokI GGATG 6 cut(s) 131, 194, 296, 346, 520, 1203
FriOI GRGCYC 1 cut(s) 484
Fsp4HI GCNGC 3 cut(s) 669, 789, 1482
FspBI CTAG 7 cut(s) 227, 290, 893, 932, 1040, 1124, 1413
GluI GCNGC 3 cut(s) 669, 789, 1482
GsuI CTGGAG 3 cut(s) 101, 1403, 1539
HaeIII GGCC 7 cut(s) 110, 389, 808, 887, 1207, 1458, 1516
HapII CCGG 1 cut(s) 852
HgaI GACGC 1 cut(s) 825
Hin1I GRCGYC 1 cut(s) 817
HincII GTYRAC 2 cut(s) 1066, 1150
HindII GTYRAC 2 cut(s) 1066, 1150
HindIII AAGCTT 2 cut(s) 453, 673
HinfI GANTC 3 cut(s) 129, 713, 737
HpaII CCGG 1 cut(s) 852
HphI GGTGA 1 cut(s) 708
Hpy166II GTNNAC 6 cut(s) 320, 526, 942, 1066, 1150, 1441
Hpy188I TCNGA 5 cut(s) 479, 712, 729, 1314, 1477
Hpy188III TCNNGA 7 cut(s) 65, 227, 270, 485, 932, 1174, 1349
Hpy8I GTNNAC 6 cut(s) 320, 526, 942, 1066, 1150, 1441
Hpy99I CGWCG 1 cut(s) 819
HpyCH4III ACNGT 1 cut(s) 643
HpyCH4IV ACGT 1 cut(s) 397
HpyF10VI GCNNNNNNNGC 4 cut(s) 488, 517, 668, 785
HpyF3I CTNAG 3 cut(s) 597, 1313, 1424
HpySE526I ACGT 1 cut(s) 397
Hsp92I GRCGYC 1 cut(s) 817
Ksp22I TGATCA 2 cut(s) 1167, 1195
LmnI GCTCC 4 cut(s) 76, 694, 1078, 1422
Lsp1109I GCAGC 3 cut(s) 680, 775, 1468
LweI GCATC 7 cut(s) 4, 109, 322, 498, 676, 1045, 1261
MaeI CTAG 7 cut(s) 227, 290, 893, 932, 1040, 1124, 1413
MaeII ACGT 1 cut(s) 397
MaeIII GTNAC 2 cut(s) 592, 1298
MboII GAAGA 3 cut(s) 413, 829, 1042
MfeI CAATTG 2 cut(s) 1199, 1397
MflI RGATCY 2 cut(s) 827, 848
MhlI GDGCHC 2 cut(s) 322, 484
MlsI TGGCCA 2 cut(s) 389, 887
MluNI TGGCCA 2 cut(s) 389, 887
MmeI TCCRAC 4 cut(s) 54, 408, 423, 480
Mox20I TGGCCA 2 cut(s) 389, 887
Mph1103I ATGCAT 3 cut(s) 29, 124, 1276
MroXI GAANNNNTTC 1 cut(s) 768
MscI TGGCCA 2 cut(s) 389, 887
MseI TTAA 4 cut(s) 162, 242, 627, 1026
MslI CAYNNNNRTG 2 cut(s) 839, 1041
Msp20I TGGCCA 2 cut(s) 389, 887
MspI CCGG 1 cut(s) 852
MspR9I CCNGG 2 cut(s) 1002, 1518
MunI CAATTG 2 cut(s) 1199, 1397
MvaI CCWGG 2 cut(s) 1002, 1518
MwoI GCNNNNNNNGC 4 cut(s) 488, 517, 668, 785
NlaIV GGNNCC 5 cut(s) 829, 850, 1074, 1418, 1430
NmuCI GTSAC 1 cut(s) 592
NsiI ATGCAT 3 cut(s) 29, 124, 1276
NspI RCATGY 5 cut(s) 126, 593, 656, 770, 916
PaeI GCATGC 1 cut(s) 656
PaeR7I CTCGAG 1 cut(s) 268
PagI TCATGA 1 cut(s) 1173
PceI AGGCCT 1 cut(s) 808
PciI ACATGT 2 cut(s) 589, 766
PdmI GAANNNNTTC 1 cut(s) 768
PfeI GAWTC 3 cut(s) 129, 713, 737
PkrI GCNGC 3 cut(s) 670, 790, 1483
PpuMI RGGWCCY 1 cut(s) 1429
PscI ACATGT 2 cut(s) 589, 766
Psp124BI GAGCTC 1 cut(s) 484
Psp5II RGGWCCY 1 cut(s) 1429
Psp6I CCWGG 2 cut(s) 1000, 1516
PspGI CCWGG 2 cut(s) 1000, 1516
PspN4I GGNNCC 5 cut(s) 829, 850, 1074, 1418, 1430
PspPI GGNCC 4 cut(s) 947, 1337, 1429, 1456
PspPPI RGGWCCY 1 cut(s) 1429
PsrI GAACNNNNNNTAC 2 cut(s) 756, 788
PsuI RGATCY 2 cut(s) 827, 848
RsaI GTAC 4 cut(s) 143, 305, 723, 941
RsaNI GTAC 4 cut(s) 142, 304, 722, 940
RseI CAYNNNNRTG 2 cut(s) 839, 1041
SacI GAGCTC 1 cut(s) 484
SaqAI TTAA 4 cut(s) 162, 242, 627, 1026
SatI GCNGC 3 cut(s) 669, 789, 1482
Sau96I GGNCC 4 cut(s) 947, 1337, 1429, 1456
ScaI AGTACT 1 cut(s) 723
ScrFI CCNGG 2 cut(s) 1002, 1518
SduI GDGCHC 2 cut(s) 322, 484
SfaNI GCATC 7 cut(s) 4, 109, 322, 498, 676, 1045, 1261
SfcI CTRYAG 1 cut(s) 982
Sfr274I CTCGAG 1 cut(s) 268
SinI GGWCC 3 cut(s) 947, 1337, 1429
SlaI CTCGAG 1 cut(s) 268
SmiMI CAYNNNNRTG 2 cut(s) 839, 1041
SmlI CTYRAG 1 cut(s) 268
SmoI CTYRAG 1 cut(s) 268
SphI GCATGC 1 cut(s) 656
SseBI AGGCCT 1 cut(s) 808
SsiI CCGC 2 cut(s) 511, 1501
SspI AATATT 1 cut(s) 1186
SspMI CTAG 7 cut(s) 227, 290, 893, 932, 1040, 1124, 1413
SstI GAGCTC 1 cut(s) 484
StuI AGGCCT 1 cut(s) 808
StyD4I CCNGG 2 cut(s) 1000, 1516
StyI CCWWGG 1 cut(s) 390
TaaI ACNGT 1 cut(s) 643
TaiI ACGT 1 cut(s) 400
TaqI TCGA 8 cut(s) 51, 99, 127, 269, 342, 548, 797, 1525
TatI WGTACW 3 cut(s) 303, 721, 939
TfiI GAWTC 3 cut(s) 129, 713, 737
Tru1I TTAA 4 cut(s) 162, 242, 627, 1026
Tru9I TTAA 4 cut(s) 162, 242, 627, 1026
TscAI CASTG 3 cut(s) 183, 669, 790
TseFI GTSAC 1 cut(s) 592
TseI GCWGC 3 cut(s) 668, 788, 1481
Tsp45I GTSAC 1 cut(s) 592
TspDTI ATGAA 8 cut(s) 224, 300, 351, 600, 750, 823, 829, 1245
TspRI CASTG 3 cut(s) 183, 669, 790
VneI GTGCAC 1 cut(s) 318
VpaK11BI GGWCC 3 cut(s) 947, 1337, 1429
XapI RAATTY 2 cut(s) 556, 952
XbaI TCTAGA 2 cut(s) 226, 931
XceI RCATGY 5 cut(s) 126, 593, 656, 770, 916
XhoI CTCGAG 1 cut(s) 268
XmiI GTMKAC 1 cut(s) 1440
XmnI GAANNNNTTC 1 cut(s) 768
XspI CTAG 7 cut(s) 227, 290, 893, 932, 1040, 1124, 1413
ZrmI AGTACT 1 cut(s) 723
Zsp2I ATGCAT 3 cut(s) 29, 124, 1276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.