Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0012851

Physical Location & Seq

Genomic Coordinates Reverse (-)

46745516 .. 46746937

Gene Structure 1422 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1349600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1422 bp

ATGGATTGGACTAGGGGCCGAACCATCGGCCTCGGCTCCTCCGCCACGGTCTCCATTGCCAAGTCACATGTCTCCGGCGAGGTTTTCGCAGTCAAGTCAGCCAAGCTCGCACAATCCCAATTCCTCCAAAAGGAGCGAGCAATTTTGTCTACGTTGAGCTCTCCCCATATTGTACATTACAAAGGCCACAACATCTCAACTGAGAACGGCGACGTTTTGTACAATTTATTTTTAGAATACGCGCCTGGTGGCACAATCTCCGACGCAATCCGACGGCAGGGCGGGTGCCTGGACGAGGCCGCGATCAGCTGCTACGCGAAGCAGATTCTGCTCGGACTGCAACACCTCCACTCCAATCAAATCGCTCACTGCGACGTCAAGTGTCGGAACATTCTGGTCGCGGAAAATGGCGCCAACGTGAAGCTCGCCGACTTGGGTTGTGCTCGCCTCGTGAATTTTGATTGTGATTCGCCGATTGGTGGGACCCCGCTGTACATGGCGCCGGAGGTGGCGCGCGGGGAGGAACAGGGGATAGCCGCCGACGTGTGGGCTCTGGGATGCACAATAATCGAGATGGCCACCGGGCGGGCGCCGTGGAACGACGTTTGCGACCCGGTATCGGCGCTGTACCGAATCGGGTTTTCCGGCGATGCGCCGGAGATACCAAGCTCTCTCTCGCAGCAAGGCAGGGACTTTGTCGCCAAGTGTTTGATTGGGGACCCACTGGAGAGGTGGTCGGCGGGTGAGCTTCTGGAGCATGGATTTGTAGTCGAGGCACCCAATGATCTTCTAAGTCCCACCACCGTGTTGGATCGTCGTTTGTGGGAAGAGGAGGAATTTGAATCGGACCAGATTTGGCATCCGACCCGTGAGAGCAGTGGTTCGGTTTATTCAGCTAAGGAGAGGATCCGAAAGTTGGGTGAAGGCATTGCGGCGTCGTCTCGACAAATTCCCAATTGGGCTTCGGATGAAGAAAATTGGGTCACAGTTAGAAGTGACAACTTCAACAAACAAGATGTAGCACAACCACACACCATTTTTCCGCTTCTCGCCGCCGGGCAGTCCAAACACGTGCCGCTGTGCCTCTTATCGCCGCCAAATGAGGCCAATATCTCGTCACAGTATACCAAAAGTATTTACGACAGGCCAACAAATACGAATCCGGATACAAATACGGATCCTAGTAGTTGCTTGGGTTTGGGTTTGGAGGCTTGTGATGCAGCTAGCTTTAGCATTAGGCATAGTAAGAGAAAGGCTTGGATGGCCTTTAATTGTAAAAGTTATGTATTTTATTCAAATCTTAATTTTGTAAACAAAAAGTTATGGTTGTTTGTGCATGCTTCTTGTTTTACCTTTTCTCACCATTTTTTAATACAGCAAACAAGTCTATTGCCTTTTGAGCTTGCCCATCTTGGTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009893 GO:0009987 GO:0010562 GO:0010604 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023052 GO:0030865 GO:0031098 GO:0031122 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0043622 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0097435 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04016 map04016

Pfam Domains

Protein Families

Pkinase

Protein Analysis

474

Amino Acids

51.97

Weight (kDa)

5.81

Isoelectric Point (pI)

53.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	4 - 256	1.4e-51	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	7 - 251	2.5e-29	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012851)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26890 AT5G55090 AT5G55090
fragaria_vesca	FvH4_3g01720
malus_domestica	MD05G1349600.v1.1 MD10G1324300.v1.1
prunus_persica	Prupe.4G017800_v2.0.a1 Prupe.5G022300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0002581
rosa_laevigata	RLG00000031041
rosa_multiflora	Rmu_co8355711.1_g000001 Rmu_sc0009084.1_g000001
rosa_rugosa	Rorug04G0397000
rosa_samantha	Rh5AG020500 Rh5BG023200 Rh5CG022500 Rh5DG021600
rosa_wichuraiana	Rw5G001890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	378
AccB1I	GGYRCC	5 cut(s)	285, 410, 499, 589, 775
AccI	GTMKAC	2 cut(s)	149, 1124
AccII	CGCG	6 cut(s)	242, 302, 317, 401, 514, 516
AccIII	TCCGGA	1 cut(s)	1162
AclWI	GGATC	5 cut(s)	819, 901, 914, 1172, 1185
AcoI	YGGCCR	1 cut(s)	576
AcsI	RAATTY	3 cut(s)	454, 836, 948
AcvI	CACGTG	1 cut(s)	1072
AcyI	GRCGYC	5 cut(s)	375, 411, 500, 590, 935
AfaI	GTAC	4 cut(s)	174, 221, 494, 629
AfiI	CCNNNNNNNGG	8 cut(s)	130, 277, 295, 479, 546, 585, 619, 916
AflIII	ACRYGT	3 cut(s)	67, 543, 1069
AgsI	TTSAA	3 cut(s)	842, 1006, 1296
AjiI	CACGTC	1 cut(s)	544
AjnI	CCWGG	2 cut(s)	244, 288
AleI	CACNNNNGTG	1 cut(s)	803
Alw21I	GWGCWC	2 cut(s)	161, 445
Alw26I	GTCTC	3 cut(s)	55, 76, 945
AlwI	GGATC	5 cut(s)	819, 901, 914, 1172, 1185
AlwNI	CAGNNNCTG	1 cut(s)	328
Aor13HI	TCCGGA	1 cut(s)	1162
AoxI	GGCC	8 cut(s)	16, 28, 184, 297, 576, 1104, 1145, 1263
ApeKI	GCWGC	3 cut(s)	309, 679, 1220
ApoI	RAATTY	3 cut(s)	454, 836, 948
AspLEI	GCGC	8 cut(s)	244, 413, 502, 514, 516, 592, 625, 655
AspS9I	GGNCC	4 cut(s)	16, 483, 718, 847
AsuC2I	CCSGG	3 cut(s)	583, 614, 1057
AsuHPI	GGTGA	3 cut(s)	755, 932, 1352
AsuNHI	GCTAGC	1 cut(s)	1223
AvaII	GGWCC	3 cut(s)	483, 718, 847
BalI	TGGCCA	1 cut(s)	578
BamHI	GGATCC	2 cut(s)	906, 1177
BanI	GGYRCC	5 cut(s)	285, 410, 499, 589, 775
BanII	GRGCYC	2 cut(s)	161, 553
BauI	CACGAG	1 cut(s)	449
BbrPI	CACGTG	1 cut(s)	1072
Bbv12I	GWGCWC	2 cut(s)	161, 445
BbvI	GCAGC	3 cut(s)	296, 691, 1232
BccI	CCATC	4 cut(s)	32, 568, 1255, 1416
BceAI	ACGGC	3 cut(s)	223, 290, 577
BcgI	CGANNNNNNTGC	2 cut(s)	550, 584
BciT130I	CCWGG	2 cut(s)	246, 290
BciVI	GTATCC	1 cut(s)	1159
BcnI	CCSGG	3 cut(s)	583, 614, 1057
BcoDI	GTCTC	3 cut(s)	55, 76, 945
BfaI	CTAG	3 cut(s)	12, 1182, 1224
BfoI	RGCGCY	4 cut(s)	414, 503, 593, 626
BfuI	GTATCC	1 cut(s)	1159
BisI	GCNGC	9 cut(s)	300, 310, 537, 680, 933, 1053, 1076, 1094, 1221
BlsI	GCNGC	9 cut(s)	301, 311, 538, 681, 934, 1054, 1077, 1095, 1222
Bme1390I	CCNGG	5 cut(s)	246, 290, 583, 614, 1057
Bme18I	GGWCC	3 cut(s)	483, 718, 847
BmgBI	CACGTC	1 cut(s)	544
BmgT120I	GGNCC	4 cut(s)	16, 483, 718, 847
BmrFI	CCNGG	5 cut(s)	246, 290, 583, 614, 1057
BmsI	GCATC	4 cut(s)	548, 640, 868, 1207
BmtI	GCTAGC	1 cut(s)	1227
BpmI	CTGGAG	2 cut(s)	746, 773
Bpu10I	CCTNAGC	1 cut(s)	897
BpuMI	CCSGG	3 cut(s)	583, 614, 1057
BsaAI	YACGTR	1 cut(s)	1072
BsaHI	GRCGYC	5 cut(s)	375, 411, 500, 590, 935
BsaI	GGTCTC	1 cut(s)	55
BsaJI	CCNNGG	3 cut(s)	31, 45, 593
BsaWI	WCCGGW	1 cut(s)	1162
BsaXI	ACNNNNNCTCC	4 cut(s)	335, 365, 719, 749
Bsc4I	CCNNNNNNNGG	8 cut(s)	130, 277, 295, 479, 546, 585, 619, 916
Bse1I	ACTGG	1 cut(s)	729
Bse3DI	GCAATG	2 cut(s)	54, 927
BseAI	TCCGGA	1 cut(s)	1162
BseBI	CCWGG	2 cut(s)	246, 290
BseDI	CCNNGG	3 cut(s)	31, 45, 593
BseGI	GGATG	4 cut(s)	563, 859, 973, 1266
BseLI	CCNNNNNNNGG	8 cut(s)	130, 277, 295, 479, 546, 585, 619, 916
BseMI	GCAATG	2 cut(s)	54, 927
BseMII	CTCAG	1 cut(s)	192
BseNI	ACTGG	1 cut(s)	729
BsePI	GCGCGC	1 cut(s)	512
BseRI	GAGGAG	2 cut(s)	28, 845
BseXI	GCAGC	3 cut(s)	296, 691, 1232
Bsh1236I	CGCG	6 cut(s)	242, 302, 317, 401, 514, 516
BshFI	GGCC	8 cut(s)	18, 30, 186, 299, 578, 1106, 1147, 1265
BshNI	GGYRCC	5 cut(s)	285, 410, 499, 589, 775
BsiHKAI	GWGCWC	2 cut(s)	161, 445
BsiSI	CCGG	8 cut(s)	75, 503, 582, 614, 645, 656, 1056, 1163
BslFI	GGGAC	4 cut(s)	496, 704, 731, 780
BslI	CCNNNNNNNGG	8 cut(s)	130, 277, 295, 479, 546, 585, 619, 916
BsmAI	GTCTC	3 cut(s)	55, 76, 945
BsmBI	CGTCTC	1 cut(s)	945
BsmFI	GGGAC	4 cut(s)	496, 704, 731, 780
BsnI	GGCC	8 cut(s)	18, 30, 186, 299, 578, 1106, 1147, 1265
Bso31I	GGTCTC	1 cut(s)	55
Bsp1286I	GDGCHC	3 cut(s)	161, 445, 553
Bsp13I	TCCGGA	1 cut(s)	1162
Bsp1407I	TGTACA	3 cut(s)	172, 219, 492
Bsp143I	GATC	5 cut(s)	303, 784, 811, 906, 1177
BspANI	GGCC	8 cut(s)	18, 30, 186, 299, 578, 1106, 1147, 1265
BspCNI	CTCAG	1 cut(s)	193
BspEI	TCCGGA	1 cut(s)	1162
BspFNI	CGCG	6 cut(s)	242, 302, 317, 401, 514, 516
BspOI	GCTAGC	1 cut(s)	1227
BspPI	GGATC	5 cut(s)	819, 901, 914, 1172, 1185
BspT107I	GGYRCC	5 cut(s)	285, 410, 499, 589, 775
BspTNI	GGTCTC	1 cut(s)	55
BsrDI	GCAATG	2 cut(s)	54, 927
BsrGI	TGTACA	3 cut(s)	172, 219, 492
BsrI	ACTGG	1 cut(s)	729
BssECI	CCNNGG	3 cut(s)	31, 45, 593
BssHII	GCGCGC	1 cut(s)	512
BssMI	GATC	5 cut(s)	303, 784, 811, 906, 1177
BssNAI	GTATAC	1 cut(s)	1125
BssNI	GRCGYC	5 cut(s)	375, 411, 500, 590, 935
BssSI	CACGAG	1 cut(s)	449
Bst1107I	GTATAC	1 cut(s)	1125
Bst2BI	CACGAG	1 cut(s)	449
Bst2UI	CCWGG	2 cut(s)	246, 290
Bst4CI	ACNGT	4 cut(s)	49, 805, 988, 1122
Bst6I	CTCTTC	1 cut(s)	822
BstACI	GRCGYC	5 cut(s)	375, 411, 500, 590, 935
BstAPI	GCANNNNNTGC	1 cut(s)	328
BstAUI	TGTACA	3 cut(s)	172, 219, 492
BstBAI	YACGTR	1 cut(s)	1072
BstC8I	GCNNGC	9 cut(s)	108, 138, 426, 445, 514, 588, 1225, 1338, 1404
BstDEI	CTNAG	3 cut(s)	201, 791, 897
BstDSI	CCRYGG	2 cut(s)	45, 593
BstENI	CCTNNNNNAGG	2 cut(s)	128, 293
BstF5I	GGATG	4 cut(s)	563, 859, 973, 1266
BstFNI	CGCG	6 cut(s)	242, 302, 317, 401, 514, 516
BstH2I	RGCGCY	4 cut(s)	414, 503, 593, 626
BstHHI	GCGC	8 cut(s)	244, 413, 502, 514, 516, 592, 625, 655
BstKTI	GATC	5 cut(s)	306, 787, 814, 909, 1180
BstMAI	GTCTC	3 cut(s)	55, 76, 945
BstMBI	GATC	5 cut(s)	303, 784, 811, 906, 1177
BstMWI	GCNNNNNNNGC	7 cut(s)	107, 328, 337, 754, 1217, 1262, 1399
BstNI	CCWGG	2 cut(s)	246, 290
BstNSI	RCATGY	2 cut(s)	71, 1340
BstSCI	CCNGG	5 cut(s)	244, 288, 581, 612, 1055
BstUI	CGCG	6 cut(s)	242, 302, 317, 401, 514, 516
BstV1I	GCAGC	3 cut(s)	296, 691, 1232
BstX2I	RGATCY	2 cut(s)	906, 1177
BstXI	CCANNNNNNTGG	1 cut(s)	808
BstYI	RGATCY	2 cut(s)	906, 1177
BstZ17I	GTATAC	1 cut(s)	1125
BsuI	GTATCC	1 cut(s)	1159
BsuRI	GGCC	8 cut(s)	18, 30, 186, 299, 578, 1106, 1147, 1265
BtgI	CCRYGG	2 cut(s)	45, 593
BtgZI	GCGATG	1 cut(s)	663
BtrI	CACGTC	1 cut(s)	544
BtsCI	GGATG	4 cut(s)	563, 859, 973, 1266
BtsI	GCAGTG	2 cut(s)	367, 883
BtsIMutI	CAGTG	3 cut(s)	367, 722, 883
Cac8I	GCNNGC	9 cut(s)	108, 138, 426, 445, 514, 588, 1225, 1338, 1404
CaiI	CAGNNNCTG	1 cut(s)	328
CfoI	GCGC	8 cut(s)	244, 413, 502, 514, 516, 592, 625, 655
Cfr13I	GGNCC	4 cut(s)	16, 483, 718, 847
CseI	GACGC	2 cut(s)	272, 924
Csp6I	GTAC	4 cut(s)	173, 220, 493, 628
CviAII	CATG	4 cut(s)	68, 496, 758, 1337
CviQI	GTAC	4 cut(s)	173, 220, 493, 628
DdeI	CTNAG	3 cut(s)	201, 791, 897
DinI	GGCGCC	3 cut(s)	412, 501, 591
DpnI	GATC	5 cut(s)	305, 786, 813, 908, 1179
DpnII	GATC	5 cut(s)	303, 784, 811, 906, 1177
EaeI	YGGCCR	1 cut(s)	576
Eam1104I	CTCTTC	1 cut(s)	822
EarI	CTCTTC	1 cut(s)	822
EciI	GGCGGA	1 cut(s)	31
Ecl136II	GAGCTC	1 cut(s)	159
Eco24I	GRGCYC	2 cut(s)	161, 553
Eco31I	GGTCTC	1 cut(s)	55
Eco47I	GGWCC	3 cut(s)	483, 718, 847
Eco53kI	GAGCTC	1 cut(s)	159
Eco72I	CACGTG	1 cut(s)	1072
EcoICRI	GAGCTC	1 cut(s)	159
EcoNI	CCTNNNNNAGG	2 cut(s)	128, 293
EcoO109I	RGGNCCY	2 cut(s)	483, 718
EcoRII	CCWGG	2 cut(s)	244, 288
EcoT38I	GRGCYC	2 cut(s)	161, 553
EgeI	GGCGCC	3 cut(s)	412, 501, 591
EheI	GGCGCC	3 cut(s)	412, 501, 591
Esp3I	CGTCTC	1 cut(s)	945
FaeI	CATG	4 cut(s)	71, 499, 761, 1340
FaqI	GGGAC	4 cut(s)	496, 704, 731, 780
FatI	CATG	4 cut(s)	67, 495, 757, 1336
FauI	CCCGC	5 cut(s)	275, 495, 509, 579, 733
FblI	GTMKAC	2 cut(s)	149, 1124
Fnu4HI	GCNGC	9 cut(s)	300, 310, 537, 680, 933, 1053, 1076, 1094, 1221
FokI	GGATG	4 cut(s)	570, 846, 980, 1273
FriOI	GRGCYC	2 cut(s)	161, 553
Fsp4HI	GCNGC	9 cut(s)	300, 310, 537, 680, 933, 1053, 1076, 1094, 1221
FspBI	CTAG	3 cut(s)	12, 1182, 1224
GlaI	GCGC	8 cut(s)	243, 412, 501, 513, 515, 591, 624, 654
GluI	GCNGC	9 cut(s)	300, 310, 537, 680, 933, 1053, 1076, 1094, 1221
GsuI	CTGGAG	2 cut(s)	746, 773
HaeII	RGCGCY	4 cut(s)	414, 503, 593, 626
HaeIII	GGCC	8 cut(s)	18, 30, 186, 299, 578, 1106, 1147, 1265
HapII	CCGG	8 cut(s)	75, 503, 582, 614, 645, 656, 1056, 1163
HgaI	GACGC	2 cut(s)	272, 924
HhaI	GCGC	8 cut(s)	244, 413, 502, 514, 516, 592, 625, 655
Hin1I	GRCGYC	5 cut(s)	375, 411, 500, 590, 935
Hin1II	CATG	4 cut(s)	71, 499, 761, 1340
Hin6I	GCGC	8 cut(s)	242, 411, 500, 512, 514, 590, 623, 653
HinP1I	GCGC	8 cut(s)	242, 411, 500, 512, 514, 590, 623, 653
HinfI	GANTC	5 cut(s)	325, 467, 633, 842, 1159
HpaII	CCGG	8 cut(s)	75, 503, 582, 614, 645, 656, 1056, 1163
HphI	GGTGA	3 cut(s)	755, 932, 1352
Hpy166II	GTNNAC	3 cut(s)	150, 1125, 1312
Hpy188I	TCNGA	8 cut(s)	262, 272, 335, 387, 847, 864, 911, 967
Hpy188III	TCNNGA	5 cut(s)	451, 571, 752, 942, 1163
Hpy8I	GTNNAC	3 cut(s)	150, 1125, 1312
Hpy99I	CGWCG	8 cut(s)	215, 266, 276, 377, 545, 605, 819, 940
HpyAV	CCTTC	1 cut(s)	917
HpyCH4III	ACNGT	4 cut(s)	49, 805, 988, 1122
HpyCH4IV	ACGT	7 cut(s)	152, 213, 375, 417, 543, 603, 1071
HpyCH4V	TGCA	5 cut(s)	340, 561, 1220, 1336, 1418
HpyF10VI	GCNNNNNNNGC	7 cut(s)	107, 328, 337, 754, 1217, 1262, 1399
HpyF3I	CTNAG	3 cut(s)	201, 791, 897
HpySE526I	ACGT	7 cut(s)	152, 213, 375, 417, 543, 603, 1071
Hsp92I	GRCGYC	5 cut(s)	375, 411, 500, 590, 935
Hsp92II	CATG	4 cut(s)	71, 499, 761, 1340
HspAI	GCGC	8 cut(s)	242, 411, 500, 512, 514, 590, 623, 653
KasI	GGCGCC	3 cut(s)	410, 499, 589
KflI	GGGWCCC	2 cut(s)	483, 718
Kpn2I	TCCGGA	1 cut(s)	1162
Kzo9I	GATC	5 cut(s)	303, 784, 811, 906, 1177
LmnI	GCTCC	3 cut(s)	41, 133, 754
Lsp1109I	GCAGC	3 cut(s)	296, 691, 1232
LweI	GCATC	4 cut(s)	548, 640, 868, 1207
MaeI	CTAG	3 cut(s)	12, 1182, 1224
MaeII	ACGT	7 cut(s)	152, 213, 375, 417, 543, 603, 1071
MaeIII	GTNAC	4 cut(s)	63, 982, 995, 1116
MalI	GATC	5 cut(s)	305, 786, 813, 908, 1179
MboI	GATC	5 cut(s)	303, 784, 811, 906, 1177
MboII	GAAGA	3 cut(s)	779, 839, 983
MfeI	CAATTG	1 cut(s)	955
MflI	RGATCY	2 cut(s)	906, 1177
MhlI	GDGCHC	3 cut(s)	161, 445, 553
MlsI	TGGCCA	1 cut(s)	578
MluNI	TGGCCA	1 cut(s)	578
Mly113I	GGCGCC	3 cut(s)	411, 500, 590
MmeI	TCCRAC	5 cut(s)	285, 295, 365, 789, 887
Mox20I	TGGCCA	1 cut(s)	578
MroI	TCCGGA	1 cut(s)	1162
MscI	TGGCCA	1 cut(s)	578
MseI	TTAA	3 cut(s)	1269, 1302, 1370
MslI	CAYNNNNRTG	2 cut(s)	803, 1413
Msp20I	TGGCCA	1 cut(s)	578
MspA1I	CMGCKG	3 cut(s)	309, 490, 1078
MspI	CCGG	8 cut(s)	75, 503, 582, 614, 645, 656, 1056, 1163
MspR9I	CCNGG	5 cut(s)	246, 290, 583, 614, 1057
MunI	CAATTG	1 cut(s)	955
MvaI	CCWGG	2 cut(s)	246, 290
MvnI	CGCG	6 cut(s)	242, 302, 317, 401, 514, 516
MwoI	GCNNNNNNNGC	7 cut(s)	107, 328, 337, 754, 1217, 1262, 1399
NarI	GGCGCC	3 cut(s)	411, 500, 590
NciI	CCSGG	3 cut(s)	583, 614, 1057
NdeII	GATC	5 cut(s)	303, 784, 811, 906, 1177
NheI	GCTAGC	1 cut(s)	1223
NlaIII	CATG	4 cut(s)	71, 499, 761, 1340
NmeAIII	GCCGAG	1 cut(s)	12
NmuCI	GTSAC	4 cut(s)	63, 982, 995, 1116
NspI	RCATGY	2 cut(s)	71, 1340
OliI	CACNNNNGTG	1 cut(s)	803
PaeI	GCATGC	1 cut(s)	1340
PauI	GCGCGC	1 cut(s)	512
PciI	ACATGT	1 cut(s)	67
PcsI	WCGNNNNNNNCGW	2 cut(s)	369, 606
PfeI	GAWTC	5 cut(s)	325, 467, 633, 842, 1159
PflFI	GACNNNGTC	1 cut(s)	695
PkrI	GCNGC	9 cut(s)	301, 311, 538, 681, 934, 1054, 1077, 1095, 1222
PluTI	GGCGCC	3 cut(s)	414, 503, 593
PmaCI	CACGTG	1 cut(s)	1072
PmlI	CACGTG	1 cut(s)	1072
Ppu21I	YACGTR	1 cut(s)	1072
PpuMI	RGGWCCY	2 cut(s)	483, 718
PscI	ACATGT	1 cut(s)	67
Psp124BI	GAGCTC	1 cut(s)	161
Psp5II	RGGWCCY	2 cut(s)	483, 718
Psp6I	CCWGG	2 cut(s)	244, 288
PspCI	CACGTG	1 cut(s)	1072
PspGI	CCWGG	2 cut(s)	244, 288
PspPI	GGNCC	4 cut(s)	16, 483, 718, 847
PspPPI	RGGWCCY	2 cut(s)	483, 718
PstNI	CAGNNNCTG	1 cut(s)	328
PsuI	RGATCY	2 cut(s)	906, 1177
PsyI	GACNNNGTC	1 cut(s)	695
PteI	GCGCGC	1 cut(s)	512
PvuII	CAGCTG	1 cut(s)	309
RsaI	GTAC	4 cut(s)	174, 221, 494, 629
RsaNI	GTAC	4 cut(s)	173, 220, 493, 628
RseI	CAYNNNNRTG	2 cut(s)	803, 1413
SacI	GAGCTC	1 cut(s)	161
SaqAI	TTAA	3 cut(s)	1269, 1302, 1370
SatI	GCNGC	9 cut(s)	300, 310, 537, 680, 933, 1053, 1076, 1094, 1221
Sau3AI	GATC	5 cut(s)	303, 784, 811, 906, 1177
Sau96I	GGNCC	4 cut(s)	16, 483, 718, 847
ScrFI	CCNGG	5 cut(s)	246, 290, 583, 614, 1057
SduI	GDGCHC	3 cut(s)	161, 445, 553
SfaNI	GCATC	4 cut(s)	548, 640, 868, 1207
SfoI	GGCGCC	3 cut(s)	412, 501, 591
SinI	GGWCC	3 cut(s)	483, 718, 847
SmiMI	CAYNNNNRTG	2 cut(s)	803, 1413
SphI	GCATGC	1 cut(s)	1340
SspDI	GGCGCC	3 cut(s)	410, 499, 589
SspMI	CTAG	3 cut(s)	12, 1182, 1224
SstI	GAGCTC	1 cut(s)	161
StyD4I	CCNGG	5 cut(s)	244, 288, 581, 612, 1055
TaaI	ACNGT	4 cut(s)	49, 805, 988, 1122
TaiI	ACGT	7 cut(s)	155, 216, 378, 420, 546, 606, 1074
TaqI	TCGA	3 cut(s)	570, 771, 943
TatI	WGTACW	3 cut(s)	172, 219, 492
TauI	GCSGC	6 cut(s)	302, 539, 935, 1055, 1078, 1096
TfiI	GAWTC	5 cut(s)	325, 467, 633, 842, 1159
Tru1I	TTAA	3 cut(s)	1269, 1302, 1370
Tru9I	TTAA	3 cut(s)	1269, 1302, 1370
TscAI	CASTG	3 cut(s)	374, 729, 883
TseFI	GTSAC	4 cut(s)	63, 982, 995, 1116
TseI	GCWGC	3 cut(s)	309, 679, 1220
Tsp45I	GTSAC	4 cut(s)	63, 982, 995, 1116
TspDTI	ATGAA	1 cut(s)	984
TspGWI	ACGGA	1 cut(s)	1190
TspRI	CASTG	3 cut(s)	374, 729, 883
Tth111I	GACNNNGTC	1 cut(s)	695
VpaK11BI	GGWCC	3 cut(s)	483, 718, 847
XagI	CCTNNNNNAGG	2 cut(s)	128, 293
XapI	RAATTY	3 cut(s)	454, 836, 948
XceI	RCATGY	2 cut(s)	71, 1340
XcmI	CCANNNNNNNNNTGG	2 cut(s)	729, 805
XmiI	GTMKAC	2 cut(s)	149, 1124
XspI	CTAG	3 cut(s)	12, 1182, 1224
ZraI	GACGTC	1 cut(s)	376

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.