Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0012851

Physical Location & Seq

Genomic Coordinates Reverse (-)

40494752 .. 40496014

Gene Structure 1263 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1324300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1263 bp

ATGGATTGGACAAGAGGCCGAACCGTCGGCCTTGGCTCCTCTGCCACAGTCTCCATCGCCAAGTCACACCGCTCCGGTGAGGTTTTCGCAGTCAAGTCGGCCGAGCTCTCACAATCCCAATTCCTCCAAAAGGAGCAAACAATTTTATCCACGTTGAGCTCTCCCTATACAGTACAGTACAAGGGTCACAACATCTCAAATGAAAACGGTGCCGTTTTGTACAATCTATTTTTAGAATACGCGCCCGGAGGCACTGTCTCCGACGCAATCCGACGGCAAGGCGGTTGCCTGAACGAGGCGGCGACAAGGTGCTACGCGAGGCAGATTCTACTCCGGCTGCAGCACCTCCATTCCAATCAAATCGCTCACTGCGACGTCAAGTGTCAGAACATTTTGGTCGCGGAAAATGGCGCGAAAGTGAAGCTCGCCGATTTGGGTTGCGCTCGCCGCGTAAATTCTGATCGTGGGTCGCCCATTGGCGGGACCCCTCTGTACATGGCGCCGGAGGTGGCGCGCTGGGAGGAACAGGGGATCGCCGCCGACGTGTGGGCTCTGGGATGTACAATAATCGAAATGACCACCGGGCGGGCTCCGTGGCCCGACGTTTGCGACCCCGTATCGGCGTTGTACCGAATCGGGTTTTCCGGCGATGCGCCGGGGATACCGAGCTCTCTGTCGAAGCAAGGCAGGGACTTTGTCGCCAAGTGTTTGATCAGGGACCCACTGGAGAGGTGGTCGGCAGTTGAGCTTCTGGAGCATGGATTTTTACTCGAGGCACCCAATGATTTGCTAAGTCCCACCACCGTACTGGATCGTGGTTTGTTGGAGGAGGAATTTGATTCGGACCAGATTTGGGATCCGACACGTGAAAGCGGTTGTTCGGATTATTCAGCTAAGGAGAGGATCCGACACTTGAGCGAAGGCAATACGTCGTCGTTAGACGTACCCAATTGGGCTTGTGATGAACATAATTGGGTCAATGTGAGGAGCAACAACATTGAAAAACAAGATGTAGCTCAAAAACACACCGCTTTTTTACCTCCTACCCGGCAATTCAAACTCTCGCTACTTTGCCCCTGTAATTTTTACGTCGAGCAAACAAGTACGTATTCAAATACAACTCTGAATACGAATAAGGATACTAGTAGTGATGCTACTAGCTTTAGCAGTAAACGTAGGAAGAGCAAGGCTTGGATGGCCTCTAAAAAAAGGTCGTACCCAGTGCACAAGGCTCCCGCTTTACGCAGGGTCTGGGAGAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009893 GO:0009987 GO:0010562 GO:0010604 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023052 GO:0030865 GO:0031098 GO:0031122 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0043622 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0097435 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04016 map04016

Pfam Domains

Protein Families

Pkinase

Protein Analysis

421

Amino Acids

46.65

Weight (kDa)

7.94

Isoelectric Point (pI)

54.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	4 - 255	4.6e-50	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	7 - 252	4.1e-26	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012851)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26890 AT5G55090 AT5G55090
fragaria_vesca	FvH4_3g01720
malus_domestica	MD05G1349600.v1.1 MD10G1324300.v1.1
prunus_persica	Prupe.4G017800_v2.0.a1 Prupe.5G022300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0002581
rosa_laevigata	RLG00000031041
rosa_multiflora	Rmu_co8355711.1_g000001 Rmu_sc0009084.1_g000001
rosa_rugosa	Rorug04G0397000
rosa_samantha	Rh5AG020500 Rh5BG023200 Rh5CG022500 Rh5DG021600
rosa_wichuraiana	Rw5G001890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	378
AccB1I	GGYRCC	3 cut(s)	209, 499, 775
AccBSI	CCGCTC	1 cut(s)	72
AccII	CGCG	6 cut(s)	242, 317, 401, 413, 450, 514
AclWI	GGATC	6 cut(s)	539, 819, 851, 864, 898, 911
AcoI	YGGCCR	1 cut(s)	99
AcsI	RAATTY	2 cut(s)	454, 833
AcvI	CACGTG	1 cut(s)	866
AcyI	GRCGYC	2 cut(s)	375, 500
AfiI	CCNNNNNNNGG	8 cut(s)	130, 295, 479, 480, 546, 585, 619, 853
AflIII	ACRYGT	2 cut(s)	543, 863
AgsI	TTSAA	3 cut(s)	1001, 1057, 1113
AhlI	ACTAGT	1 cut(s)	1142
AjiI	CACGTC	1 cut(s)	544
AluBI	AGCT	8 cut(s)	106, 159, 424, 669, 748, 893, 1016, 1161
AluI	AGCT	8 cut(s)	106, 159, 424, 669, 748, 893, 1016, 1161
Alw21I	GWGCWC	4 cut(s)	108, 161, 671, 1227
Alw26I	GTCTC	2 cut(s)	55, 262
Alw44I	GTGCAC	1 cut(s)	1223
AlwI	GGATC	6 cut(s)	539, 819, 851, 864, 898, 911
AlwNI	CAGNNNCTG	1 cut(s)	1251
Ama87I	CYCGRG	1 cut(s)	770
AoxI	GGCC	5 cut(s)	16, 28, 99, 596, 1197
ApaLI	GTGCAC	1 cut(s)	1223
ApeKI	GCWGC	2 cut(s)	337, 340
ApoI	RAATTY	2 cut(s)	454, 833
AspLEI	GCGC	7 cut(s)	244, 413, 443, 502, 514, 516, 655
AspS9I	GGNCC	4 cut(s)	483, 597, 718, 844
AsuC2I	CCSGG	4 cut(s)	246, 583, 657, 1048
AsuHPI	GGTGA	1 cut(s)	89
AvaI	CYCGRG	1 cut(s)	770
AvaII	GGWCC	3 cut(s)	483, 718, 844
BaeGI	GKGCMC	1 cut(s)	1227
BamHI	GGATCC	2 cut(s)	856, 903
BanI	GGYRCC	3 cut(s)	209, 499, 775
BanII	GRGCYC	5 cut(s)	108, 161, 553, 592, 671
BbrPI	CACGTG	1 cut(s)	866
Bbv12I	GWGCWC	4 cut(s)	108, 161, 671, 1227
BbvI	GCAGC	2 cut(s)	324, 352
BccI	CCATC	2 cut(s)	62, 1189
BceAI	ACGGC	2 cut(s)	197, 290
BcgI	CGANNNNNNTGC	2 cut(s)	78, 112
BciVI	GTATCC	2 cut(s)	654, 1132
BclI	TGATCA	1 cut(s)	711
BcnI	CCSGG	4 cut(s)	246, 583, 657, 1048
BcoDI	GTCTC	2 cut(s)	55, 262
BcuI	ACTAGT	1 cut(s)	1142
BfaI	CTAG	2 cut(s)	1143, 1158
BfmI	CTRYAG	1 cut(s)	338
BfoI	RGCGCY	1 cut(s)	503
BfuI	GTATCC	2 cut(s)	654, 1132
BisI	GCNGC	5 cut(s)	300, 338, 341, 448, 537
BlsI	GCNGC	5 cut(s)	301, 339, 342, 449, 538
Bme1390I	CCNGG	4 cut(s)	246, 583, 657, 1048
Bme18I	GGWCC	3 cut(s)	483, 718, 844
BmeT110I	CYCGRG	1 cut(s)	770
BmgBI	CACGTC	1 cut(s)	544
BmgT120I	GGNCC	4 cut(s)	483, 597, 718, 844
BmrFI	CCNGG	4 cut(s)	246, 583, 657, 1048
BmrI	ACTGGG	1 cut(s)	1214
BmsI	GCATC	2 cut(s)	640, 1141
BmuI	ACTGGG	1 cut(s)	1214
BpmI	CTGGAG	2 cut(s)	746, 773
Bpu10I	CCTNAGC	1 cut(s)	894
BpuEI	CTTGAG	1 cut(s)	934
BpuMI	CCSGG	4 cut(s)	246, 583, 657, 1048
BsaAI	YACGTR	2 cut(s)	866, 1107
BsaHI	GRCGYC	2 cut(s)	375, 500
BsaJI	CCNNGG	3 cut(s)	31, 593, 656
BsaWI	WCCGGW	1 cut(s)	74
BsaXI	ACNNNNNCTCC	4 cut(s)	240, 270, 719, 749
Bsc4I	CCNNNNNNNGG	8 cut(s)	130, 295, 479, 480, 546, 585, 619, 853
Bse1I	ACTGG	3 cut(s)	729, 813, 1220
BseDI	CCNNGG	3 cut(s)	31, 593, 656
BseGI	GGATG	2 cut(s)	563, 1200
BseLI	CCNNNNNNNGG	8 cut(s)	130, 295, 479, 480, 546, 585, 619, 853
BseNI	ACTGG	3 cut(s)	729, 813, 1220
BsePI	GCGCGC	1 cut(s)	512
BseRI	GAGGAG	3 cut(s)	28, 842, 1000
BseSI	GKGCMC	1 cut(s)	1227
BseX3I	CGGCCG	1 cut(s)	99
BseXI	GCAGC	2 cut(s)	324, 352
BseYI	CCCAGC	1 cut(s)	516
Bsh1236I	CGCG	6 cut(s)	242, 317, 401, 413, 450, 514
Bsh1285I	CGRYCG	1 cut(s)	102
BshFI	GGCC	5 cut(s)	18, 30, 101, 598, 1199
BshNI	GGYRCC	3 cut(s)	209, 499, 775
BsiEI	CGRYCG	1 cut(s)	102
BsiHKAI	GWGCWC	4 cut(s)	108, 161, 671, 1227
BsiHKCI	CYCGRG	1 cut(s)	770
BsiSI	CCGG	8 cut(s)	75, 246, 334, 503, 582, 645, 656, 1048
BslFI	GGGAC	4 cut(s)	496, 704, 731, 780
BslI	CCNNNNNNNGG	8 cut(s)	130, 295, 479, 480, 546, 585, 619, 853
BsmAI	GTCTC	2 cut(s)	55, 262
BsmFI	GGGAC	4 cut(s)	496, 704, 731, 780
BsnI	GGCC	5 cut(s)	18, 30, 101, 598, 1199
BsoBI	CYCGRG	1 cut(s)	770
Bsp1286I	GDGCHC	6 cut(s)	108, 161, 553, 592, 671, 1227
Bsp1407I	TGTACA	3 cut(s)	219, 492, 560
Bsp143I	GATC	6 cut(s)	460, 531, 711, 811, 856, 903
BspANI	GGCC	5 cut(s)	18, 30, 101, 598, 1199
BspFNI	CGCG	6 cut(s)	242, 317, 401, 413, 450, 514
BspMAI	CTGCAG	1 cut(s)	342
BspPI	GGATC	6 cut(s)	539, 819, 851, 864, 898, 911
BspQI	GCTCTTC	1 cut(s)	1175
BspT107I	GGYRCC	3 cut(s)	209, 499, 775
BsrBI	CCGCTC	1 cut(s)	72
BsrGI	TGTACA	3 cut(s)	219, 492, 560
BsrI	ACTGG	3 cut(s)	729, 813, 1220
BssECI	CCNNGG	3 cut(s)	31, 593, 656
BssHII	GCGCGC	1 cut(s)	512
BssMI	GATC	6 cut(s)	460, 531, 711, 811, 856, 903
BssNI	GRCGYC	2 cut(s)	375, 500
BssT1I	CCWWGG	1 cut(s)	31
Bst4CI	ACNGT	7 cut(s)	25, 49, 172, 177, 209, 256, 805
Bst6I	CTCTTC	1 cut(s)	1175
BstACI	GRCGYC	2 cut(s)	375, 500
BstAUI	TGTACA	3 cut(s)	219, 492, 560
BstBAI	YACGTR	2 cut(s)	866, 1107
BstC8I	GCNNGC	4 cut(s)	426, 445, 514, 588
BstDEI	CTNAG	2 cut(s)	791, 894
BstDSI	CCRYGG	1 cut(s)	593
BstENI	CCTNNNNNAGG	2 cut(s)	128, 293
BstF5I	GGATG	2 cut(s)	563, 1200
BstFNI	CGCG	6 cut(s)	242, 317, 401, 413, 450, 514
BstH2I	RGCGCY	1 cut(s)	503
BstHHI	GCGC	7 cut(s)	244, 413, 443, 502, 514, 516, 655
BstKTI	GATC	6 cut(s)	463, 534, 714, 814, 859, 906
BstMAI	GTCTC	2 cut(s)	55, 262
BstMBI	GATC	6 cut(s)	460, 531, 711, 811, 856, 903
BstMCI	CGRYCG	1 cut(s)	102
BstMWI	GCNNNNNNNGC	3 cut(s)	447, 754, 1196
BstSCI	CCNGG	4 cut(s)	244, 581, 655, 1046
BstSFI	CTRYAG	1 cut(s)	338
BstSLI	GKGCMC	1 cut(s)	1227
BstSNI	TACGTA	1 cut(s)	1107
BstUI	CGCG	6 cut(s)	242, 317, 401, 413, 450, 514
BstV1I	GCAGC	2 cut(s)	324, 352
BstX2I	RGATCY	2 cut(s)	856, 903
BstXI	CCANNNNNNTGG	1 cut(s)	808
BstYI	RGATCY	2 cut(s)	856, 903
BstZI	CGGCCG	1 cut(s)	99
BsuI	GTATCC	2 cut(s)	654, 1132
BsuRI	GGCC	5 cut(s)	18, 30, 101, 598, 1199
BtgI	CCRYGG	1 cut(s)	593
BtgZI	GCGATG	2 cut(s)	40, 663
BtrI	CACGTC	1 cut(s)	544
BtsCI	GGATG	2 cut(s)	563, 1200
BtsI	GCAGTG	1 cut(s)	367
BtsIMutI	CAGTG	4 cut(s)	252, 367, 722, 1227
Cac8I	GCNNGC	4 cut(s)	426, 445, 514, 588
CaiI	CAGNNNCTG	1 cut(s)	1251
CfoI	GCGC	7 cut(s)	244, 413, 443, 502, 514, 516, 655
Cfr13I	GGNCC	4 cut(s)	483, 597, 718, 844
CseI	GACGC	1 cut(s)	272
CviAII	CATG	2 cut(s)	496, 758
DdeI	CTNAG	2 cut(s)	791, 894
DinI	GGCGCC	1 cut(s)	501
DpnI	GATC	6 cut(s)	462, 533, 713, 813, 858, 905
DpnII	GATC	6 cut(s)	460, 531, 711, 811, 856, 903
EaeI	YGGCCR	1 cut(s)	99
EagI	CGGCCG	1 cut(s)	99
Eam1104I	CTCTTC	1 cut(s)	1175
EarI	CTCTTC	1 cut(s)	1175
Ecl136II	GAGCTC	3 cut(s)	106, 159, 669
EclXI	CGGCCG	1 cut(s)	99
Eco105I	TACGTA	1 cut(s)	1107
Eco130I	CCWWGG	1 cut(s)	31
Eco24I	GRGCYC	5 cut(s)	108, 161, 553, 592, 671
Eco47I	GGWCC	3 cut(s)	483, 718, 844
Eco52I	CGGCCG	1 cut(s)	99
Eco53kI	GAGCTC	3 cut(s)	106, 159, 669
Eco72I	CACGTG	1 cut(s)	866
Eco88I	CYCGRG	1 cut(s)	770
EcoICRI	GAGCTC	3 cut(s)	106, 159, 669
EcoNI	CCTNNNNNAGG	2 cut(s)	128, 293
EcoO109I	RGGNCCY	2 cut(s)	483, 718
EcoT14I	CCWWGG	1 cut(s)	31
EcoT38I	GRGCYC	5 cut(s)	108, 161, 553, 592, 671
EgeI	GGCGCC	1 cut(s)	501
EheI	GGCGCC	1 cut(s)	501
ErhI	CCWWGG	1 cut(s)	31
FaeI	CATG	2 cut(s)	499, 761
FaiI	YATR	4 cut(s)	168, 497, 759, 969
FaqI	GGGAC	4 cut(s)	496, 704, 731, 780
FatI	CATG	2 cut(s)	495, 757
FauI	CCCGC	3 cut(s)	473, 579, 1243
FbaI	TGATCA	1 cut(s)	711
Fnu4HI	GCNGC	5 cut(s)	300, 338, 341, 448, 537
FokI	GGATG	2 cut(s)	570, 1207
FriOI	GRGCYC	5 cut(s)	108, 161, 553, 592, 671
Fsp4HI	GCNGC	5 cut(s)	300, 338, 341, 448, 537
FspBI	CTAG	2 cut(s)	1143, 1158
GlaI	GCGC	7 cut(s)	243, 412, 442, 501, 513, 515, 654
GluI	GCNGC	5 cut(s)	300, 338, 341, 448, 537
GsaI	CCCAGC	1 cut(s)	520
GsuI	CTGGAG	2 cut(s)	746, 773
HaeII	RGCGCY	1 cut(s)	503
HaeIII	GGCC	5 cut(s)	18, 30, 101, 598, 1199
HapII	CCGG	8 cut(s)	75, 246, 334, 503, 582, 645, 656, 1048
HgaI	GACGC	1 cut(s)	272
HhaI	GCGC	7 cut(s)	244, 413, 443, 502, 514, 516, 655
Hin1I	GRCGYC	2 cut(s)	375, 500
Hin1II	CATG	2 cut(s)	499, 761
Hin6I	GCGC	7 cut(s)	242, 411, 441, 500, 512, 514, 653
HinP1I	GCGC	7 cut(s)	242, 411, 441, 500, 512, 514, 653
HinfI	GANTC	3 cut(s)	325, 633, 839
HpaII	CCGG	8 cut(s)	75, 246, 334, 503, 582, 645, 656, 1048
HphI	GGTGA	1 cut(s)	89
Hpy166II	GTNNAC	2 cut(s)	1172, 1225
Hpy188I	TCNGA	9 cut(s)	262, 272, 387, 460, 844, 861, 883, 908, 1125
Hpy188III	TCNNGA	1 cut(s)	752
Hpy8I	GTNNAC	2 cut(s)	1172, 1225
Hpy99I	CGWCG	9 cut(s)	29, 266, 276, 377, 545, 605, 934, 937, 1094
HpyAV	CCTTC	1 cut(s)	914
HpyCH4III	ACNGT	7 cut(s)	25, 49, 172, 177, 209, 256, 805
HpyCH4V	TGCA	2 cut(s)	340, 1225
HpyF10VI	GCNNNNNNNGC	3 cut(s)	447, 754, 1196
HpyF3I	CTNAG	2 cut(s)	791, 894
Hsp92I	GRCGYC	2 cut(s)	375, 500
Hsp92II	CATG	2 cut(s)	499, 761
HspAI	GCGC	7 cut(s)	242, 411, 441, 500, 512, 514, 653
KasI	GGCGCC	1 cut(s)	499
KflI	GGGWCCC	2 cut(s)	483, 718
Ksp22I	TGATCA	1 cut(s)	711
Kzo9I	GATC	6 cut(s)	460, 531, 711, 811, 856, 903
LguI	GCTCTTC	1 cut(s)	1175
LmnI	GCTCC	7 cut(s)	41, 77, 133, 595, 754, 987, 1237
Lsp1109I	GCAGC	2 cut(s)	324, 352
LweI	GCATC	2 cut(s)	640, 1141
MaeI	CTAG	2 cut(s)	1143, 1158
MaeIII	GTNAC	2 cut(s)	63, 185
MalI	GATC	6 cut(s)	462, 533, 713, 813, 858, 905
MbiI	CCGCTC	1 cut(s)	72
MboI	GATC	6 cut(s)	460, 531, 711, 811, 856, 903
MboII	GAAGA	1 cut(s)	1192
MfeI	CAATTG	1 cut(s)	949
MflI	RGATCY	2 cut(s)	856, 903
MhlI	GDGCHC	6 cut(s)	108, 161, 553, 592, 671, 1227
MluCI	AATT	8 cut(s)	119, 141, 454, 833, 949, 970, 1052, 1081
Mly113I	GGCGCC	1 cut(s)	500
MmeI	TCCRAC	5 cut(s)	285, 295, 804, 884, 931
MspI	CCGG	8 cut(s)	75, 246, 334, 503, 582, 645, 656, 1048
MspR9I	CCNGG	4 cut(s)	246, 583, 657, 1048
MunI	CAATTG	1 cut(s)	949
MvnI	CGCG	6 cut(s)	242, 317, 401, 413, 450, 514
MwoI	GCNNNNNNNGC	3 cut(s)	447, 754, 1196
NarI	GGCGCC	1 cut(s)	500
NciI	CCSGG	4 cut(s)	246, 583, 657, 1048
NdeII	GATC	6 cut(s)	460, 531, 711, 811, 856, 903
NlaIII	CATG	2 cut(s)	499, 761
NmeAIII	GCCGAG	1 cut(s)	127
NmuCI	GTSAC	2 cut(s)	63, 185
PaeR7I	CTCGAG	1 cut(s)	770
PauI	GCGCGC	1 cut(s)	512
PciSI	GCTCTTC	1 cut(s)	1175
PcsI	WCGNNNNNNNCGW	2 cut(s)	369, 540
PfeI	GAWTC	3 cut(s)	325, 633, 839
PflFI	GACNNNGTC	1 cut(s)	695
PkrI	GCNGC	5 cut(s)	301, 339, 342, 449, 538
PluTI	GGCGCC	1 cut(s)	503
PmaCI	CACGTG	1 cut(s)	866
PmlI	CACGTG	1 cut(s)	866
Ppu21I	YACGTR	2 cut(s)	866, 1107
PpuMI	RGGWCCY	2 cut(s)	483, 718
Psp124BI	GAGCTC	3 cut(s)	108, 161, 671
Psp5II	RGGWCCY	2 cut(s)	483, 718
PspCI	CACGTG	1 cut(s)	866
PspFI	CCCAGC	1 cut(s)	516
PspPI	GGNCC	4 cut(s)	483, 597, 718, 844
PspPPI	RGGWCCY	2 cut(s)	483, 718
PspXI	VCTCGAGB	1 cut(s)	770
PstI	CTGCAG	1 cut(s)	342
PstNI	CAGNNNCTG	1 cut(s)	1251
PsuI	RGATCY	2 cut(s)	856, 903
PsyI	GACNNNGTC	1 cut(s)	695
PteI	GCGCGC	1 cut(s)	512
SacI	GAGCTC	3 cut(s)	108, 161, 671
SapI	GCTCTTC	1 cut(s)	1175
SatI	GCNGC	5 cut(s)	300, 338, 341, 448, 537
Sau3AI	GATC	6 cut(s)	460, 531, 711, 811, 856, 903
Sau96I	GGNCC	4 cut(s)	483, 597, 718, 844
ScrFI	CCNGG	4 cut(s)	246, 583, 657, 1048
SduI	GDGCHC	6 cut(s)	108, 161, 553, 592, 671, 1227
SfaNI	GCATC	2 cut(s)	640, 1141
SfcI	CTRYAG	1 cut(s)	338
SfoI	GGCGCC	1 cut(s)	501
Sfr274I	CTCGAG	1 cut(s)	770
SinI	GGWCC	3 cut(s)	483, 718, 844
SlaI	CTCGAG	1 cut(s)	770
SmlI	CTYRAG	2 cut(s)	770, 913
SmoI	CTYRAG	2 cut(s)	770, 913
SnaBI	TACGTA	1 cut(s)	1107
SpeI	ACTAGT	1 cut(s)	1142
Sse9I	AATT	8 cut(s)	119, 141, 454, 833, 949, 970, 1052, 1081
SspDI	GGCGCC	1 cut(s)	499
SspMI	CTAG	2 cut(s)	1143, 1158
SstI	GAGCTC	3 cut(s)	108, 161, 671
StyD4I	CCNGG	4 cut(s)	244, 581, 655, 1046
StyI	CCWWGG	1 cut(s)	31
TaaI	ACNGT	7 cut(s)	25, 49, 172, 177, 209, 256, 805
TaqI	TCGA	4 cut(s)	570, 677, 771, 1092
TasI	AATT	8 cut(s)	119, 141, 454, 833, 949, 970, 1052, 1081
TatI	WGTACW	5 cut(s)	172, 177, 219, 492, 560
TauI	GCSGC	3 cut(s)	302, 450, 539
TfiI	GAWTC	3 cut(s)	325, 633, 839
TscAI	CASTG	4 cut(s)	259, 374, 729, 1227
TseFI	GTSAC	2 cut(s)	63, 185
TseI	GCWGC	2 cut(s)	337, 340
Tsp45I	GTSAC	2 cut(s)	63, 185
TspDTI	ATGAA	2 cut(s)	216, 978
TspGWI	ACGGA	1 cut(s)	582
TspRI	CASTG	4 cut(s)	259, 374, 729, 1227
Tth111I	GACNNNGTC	1 cut(s)	695
VneI	GTGCAC	1 cut(s)	1223
VpaK11BI	GGWCC	3 cut(s)	483, 718, 844
XagI	CCTNNNNNAGG	2 cut(s)	128, 293
XapI	RAATTY	2 cut(s)	454, 833
XcmI	CCANNNNNNNNNTGG	2 cut(s)	729, 805
XhoI	CTCGAG	1 cut(s)	770
XspI	CTAG	2 cut(s)	1143, 1158
ZraI	GACGTC	1 cut(s)	376

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.