Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0012851

Physical Location & Seq

Genomic Coordinates Forward (+)

1554952 .. 1556826

Gene Structure 1875 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG021600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1269 bp

ATGAACTGGACCAGAGGCCGTACAATCGGCGTTGGCTCCTCGGCCACCGTCTCTATAGCCAGGTCTTGTGATTCCGGGGAGGTTTTCGTGGTGAAGTCAGCAAGTCTCTCACATTCTCACTCCCTTCAGGTGGAGCAAGAGCTTCTGTCCACTTTGAGCTGCGCTCAAATAGTGTCGTACAAGGGCTGCAACATCTCCACCGAAAACGGCCGCGTTCTCTACAATCTCTGCCTCGAGTACGCACCGGGCGGCACGCTTTCCGATGAAGTTCGAAGGCAAGGCGGGTCGTTAAGCGAGTCCACGATGAGATCCTACACGAGGCAGATACTATTGGGGATGCAGTACCTACATGCTAATGGAATTGTACATTGTGACATTAAGGGTCACAATATTTTGGTGACGGAAGAGGGCGCGAAGTTGGCGGATTTGGGTTGTGCTCGACGCGTAAATGGTGATCATGGCTCCGCTACCATTAGTGGCACTCCTCTTTACATGGCACCGGAGGCAGCGCGTGGAGAGCAACAAGGGTTCCCGGCCGATGTGTGGGCCCTAGGATGTACAATAATCGAGATGGCTACGGGGCAAGTCCCGTGGCCTAATGTTTCGGACCCGGTATCGGGTTTGTACCGGATCGGGTTTTCGGGTGATGTGCCCGAGATTCCAGACTCTTTGTCAAAGCAAGGTAGGGAATTTGTGAGCAAGTGTTTGAAGAGAGACCCATTGGAGAGGTGGTCAGCTAGTGAGCTGCTCAAGCATTCTTTTCTTCTCGAGGAAGAGGAATCCTATTCTTGGAATAGTCCCACAACTGTGTTGGATCATGGTTTGTGGGACTTGGAGGAGTTTGATGATATGGAATGGGAACAAAGTACCTGTTCAAATTCAGCTAGGGAAAGAATTAGAAAGTTGAGTGAAGGGAATGCCGAGACGACGTCGTTAGTCGGTGGAATGCTCAATTGGGCTTGTGATGGAGATTGGGTGACGGTGAGAAGCAACAAGGAGGTTAATATATTGCCAGAAGATGCCAATCAAGATTATGAAGATGAGGCAACAAATACAAATTCTAGTGCTAGTAGTAATGGGTTGGGTTTGGGCACTCGTAATAGTAGTTTTAGTTGTAGGGATCGGAAGTATTGGCCTTTGAAGGCCTCAAATTCCAAATGTAGCAAAAATGTGTTCTGTATAAACCAAAACTTTGATAATGAGGAATTTTGGTTTTTTGTGCATGACATACTTAATGTTGTCGATCTGTTTAAGGCCCTAGTTAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009893 GO:0009987 GO:0010562 GO:0010604 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023052 GO:0030865 GO:0031098 GO:0031122 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0043622 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0097435 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04016 map04016

Pfam Domains

Protein Families

Pkinase

Protein Analysis

422

Amino Acids

46.59

Weight (kDa)

4.94

Isoelectric Point (pI)

45.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	4 - 255	2.5e-55	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	7 - 251	1.2e-30	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012851)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26890 AT5G55090 AT5G55090
fragaria_vesca	FvH4_3g01720
malus_domestica	MD05G1349600.v1.1 MD10G1324300.v1.1
prunus_persica	Prupe.4G017800_v2.0.a1 Prupe.5G022300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0002581
rosa_laevigata	RLG00000031041
rosa_multiflora	Rmu_co8355711.1_g000001 Rmu_sc0009084.1_g000001
rosa_rugosa	Rorug04G0397000
rosa_samantha	Rh5AG020500 Rh5BG023200 Rh5CG022500 Rh5DG021600
rosa_wichuraiana	Rw5G001890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	932
AccB1I	GGYRCC	1 cut(s)	496
AccII	CGCG	4 cut(s)	213, 413, 444, 511
AciI	CCGC	5 cut(s)	211, 249, 282, 422, 465
AclWI	GGATC	4 cut(s)	303, 638, 822, 1128
AcoI	YGGCCR	3 cut(s)	42, 208, 534
AcsI	RAATTY	5 cut(s)	689, 877, 1057, 1150, 1205
AcuI	CTGAAG	1 cut(s)	110
AcyI	GRCGYC	1 cut(s)	929
AfaI	GTAC	8 cut(s)	22, 179, 239, 344, 366, 559, 626, 868
AfiI	CCNNNNNNNGG	6 cut(s)	130, 318, 543, 616, 617, 789
AflIII	ACRYGT	1 cut(s)	442
AgsI	TTSAA	3 cut(s)	709, 876, 1141
AhdI	GACNNNNNGTC	1 cut(s)	670
AjnI	CCWGG	1 cut(s)	59
AjuI	GAANNNNNNNTTGG	2 cut(s)	937, 969
AleI	CACNNNNGTG	1 cut(s)	806
AluBI	AGCT	5 cut(s)	142, 159, 737, 745, 884
AluI	AGCT	5 cut(s)	142, 159, 737, 745, 884
Alw21I	GWGCWC	1 cut(s)	439
Alw26I	GTCTC	4 cut(s)	55, 110, 708, 917
AlwI	GGATC	4 cut(s)	303, 638, 822, 1128
Ama87I	CYCGRG	3 cut(s)	233, 653, 767
AoxI	GGCC	9 cut(s)	16, 42, 208, 534, 546, 593, 1133, 1143, 1254
ApaI	GGGCCC	1 cut(s)	550
ApeKI	GCWGC	4 cut(s)	159, 186, 506, 745
ApoI	RAATTY	5 cut(s)	689, 877, 1057, 1150, 1205
AspA2I	CCTAGG	1 cut(s)	550
AspLEI	GCGC	3 cut(s)	164, 413, 511
AspS9I	GGNCC	5 cut(s)	9, 546, 547, 607, 1255
AsuC2I	CCSGG	4 cut(s)	76, 246, 533, 611
AsuHPI	GGTGA	6 cut(s)	103, 409, 464, 656, 988, 994
AsuII	TTCGAA	1 cut(s)	271
AvaI	CYCGRG	3 cut(s)	233, 653, 767
AvaII	GGWCC	2 cut(s)	9, 607
AvrII	CCTAGG	1 cut(s)	550
BaeGI	GKGCMC	3 cut(s)	550, 654, 1094
BanI	GGYRCC	1 cut(s)	496
BanII	GRGCYC	1 cut(s)	550
BauI	CACGAG	1 cut(s)	316
Bbv12I	GWGCWC	1 cut(s)	439
BbvI	GCAGC	4 cut(s)	146, 173, 518, 732
BccI	CCATC	2 cut(s)	565, 959
BceAI	ACGGC	2 cut(s)	3, 223
BciT130I	CCWGG	1 cut(s)	61
BclI	TGATCA	1 cut(s)	454
BcnI	CCSGG	4 cut(s)	76, 246, 533, 611
BcoDI	GTCTC	4 cut(s)	55, 110, 708, 917
BfaI	CTAG	6 cut(s)	551, 738, 885, 1062, 1068, 1259
BfmI	CTRYAG	1 cut(s)	54
BisI	GCNGC	6 cut(s)	160, 187, 211, 250, 507, 746
BlnI	CCTAGG	1 cut(s)	550
BlsI	GCNGC	6 cut(s)	161, 188, 212, 251, 508, 747
Bme1390I	CCNGG	5 cut(s)	61, 76, 246, 533, 611
Bme18I	GGWCC	2 cut(s)	9, 607
BmeRI	GACNNNNNGTC	1 cut(s)	670
BmeT110I	CYCGRG	3 cut(s)	233, 653, 767
BmgT120I	GGNCC	5 cut(s)	9, 546, 547, 607, 1255
BmiI	GGNNCC	6 cut(s)	37, 463, 498, 530, 548, 609
BmrFI	CCNGG	5 cut(s)	61, 76, 246, 533, 611
BmsI	GCATC	2 cut(s)	327, 1009
BplI	GAGNNNNNCTC	2 cut(s)	148, 180
Bpu14I	TTCGAA	1 cut(s)	271
BpuEI	CTTGAG	1 cut(s)	734
BpuMI	CCSGG	4 cut(s)	76, 246, 533, 611
BsaBI	GATNNNNATC	1 cut(s)	1023
BsaHI	GRCGYC	1 cut(s)	929
BsaI	GGTCTC	1 cut(s)	708
BsaJI	CCNNGG	4 cut(s)	39, 75, 550, 590
BsaWI	WCCGGW	2 cut(s)	499, 627
BsaXI	ACNNNNNCTCC	4 cut(s)	716, 746, 960, 990
Bsc4I	CCNNNNNNNGG	6 cut(s)	130, 318, 543, 616, 617, 789
Bse1I	ACTGG	1 cut(s)	11
Bse8I	GATNNNNATC	1 cut(s)	1023
BseBI	CCWGG	1 cut(s)	61
BseDI	CCNNGG	4 cut(s)	39, 75, 550, 590
BseGI	GGATG	2 cut(s)	342, 560
BseJI	GATNNNNATC	1 cut(s)	1023
BseLI	CCNNNNNNNGG	6 cut(s)	130, 318, 543, 616, 617, 789
BseNI	ACTGG	1 cut(s)	11
BseRI	GAGGAG	3 cut(s)	28, 474, 851
BseSI	GKGCMC	3 cut(s)	550, 654, 1094
BseX3I	CGGCCG	2 cut(s)	208, 534
BseXI	GCAGC	4 cut(s)	146, 173, 518, 732
Bsh1236I	CGCG	4 cut(s)	213, 413, 444, 511
Bsh1285I	CGRYCG	2 cut(s)	211, 537
BshFI	GGCC	9 cut(s)	18, 44, 210, 536, 548, 595, 1135, 1145, 1256
BshNI	GGYRCC	1 cut(s)	496
BsiEI	CGRYCG	2 cut(s)	211, 537
BsiHKAI	GWGCWC	1 cut(s)	439
BsiHKCI	CYCGRG	3 cut(s)	233, 653, 767
BsiSI	CCGG	6 cut(s)	75, 245, 500, 533, 611, 628
BslFI	GGGAC	3 cut(s)	572, 783, 842
BslI	CCNNNNNNNGG	6 cut(s)	130, 318, 543, 616, 617, 789
BsmAI	GTCTC	4 cut(s)	55, 110, 708, 917
BsmBI	CGTCTC	2 cut(s)	55, 917
BsmFI	GGGAC	3 cut(s)	572, 783, 842
BsmI	GAATGC	3 cut(s)	754, 922, 951
BsnI	GGCC	9 cut(s)	18, 44, 210, 536, 548, 595, 1135, 1145, 1256
Bso31I	GGTCTC	1 cut(s)	708
BsoBI	CYCGRG	3 cut(s)	233, 653, 767
Bsp119I	TTCGAA	1 cut(s)	271
Bsp120I	GGGCCC	1 cut(s)	546
Bsp1286I	GDGCHC	4 cut(s)	439, 550, 654, 1094
Bsp1407I	TGTACA	2 cut(s)	364, 557
Bsp143I	GATC	6 cut(s)	308, 454, 630, 814, 1120, 1243
BspACI	CCGC	5 cut(s)	211, 249, 282, 422, 465
BspANI	GGCC	9 cut(s)	18, 44, 210, 536, 548, 595, 1135, 1145, 1256
BspFNI	CGCG	4 cut(s)	213, 413, 444, 511
BspLI	GGNNCC	6 cut(s)	37, 463, 498, 530, 548, 609
BspPI	GGATC	4 cut(s)	303, 638, 822, 1128
BspT104I	TTCGAA	1 cut(s)	271
BspT107I	GGYRCC	1 cut(s)	496
BspTNI	GGTCTC	1 cut(s)	708
BsrGI	TGTACA	2 cut(s)	364, 557
BsrI	ACTGG	1 cut(s)	11
BssECI	CCNNGG	4 cut(s)	39, 75, 550, 590
BssMI	GATC	6 cut(s)	308, 454, 630, 814, 1120, 1243
BssNI	GRCGYC	1 cut(s)	929
BssSI	CACGAG	1 cut(s)	316
BssT1I	CCWWGG	1 cut(s)	550
Bst2BI	CACGAG	1 cut(s)	316
Bst2UI	CCWGG	1 cut(s)	61
Bst4CI	ACNGT	3 cut(s)	49, 808, 982
Bst6I	CTCTTC	3 cut(s)	399, 704, 768
BstACI	GRCGYC	1 cut(s)	929
BstAUI	TGTACA	2 cut(s)	364, 557
BstBI	TTCGAA	1 cut(s)	271
BstC8I	GCNNGC	1 cut(s)	254
BstDSI	CCRYGG	1 cut(s)	590
BstENI	CCTNNNNNAGG	1 cut(s)	316
BstF5I	GGATG	2 cut(s)	342, 560
BstFNI	CGCG	4 cut(s)	213, 413, 444, 511
BstHHI	GCGC	3 cut(s)	164, 413, 511
BstKTI	GATC	6 cut(s)	311, 457, 633, 817, 1123, 1246
BstMAI	GTCTC	4 cut(s)	55, 110, 708, 917
BstMBI	GATC	6 cut(s)	308, 454, 630, 814, 1120, 1243
BstMCI	CGRYCG	2 cut(s)	211, 537
BstMWI	GCNNNNNNNGC	4 cut(s)	419, 503, 517, 751
BstNI	CCWGG	1 cut(s)	61
BstNSI	RCATGY	1 cut(s)	353
BstSCI	CCNGG	5 cut(s)	59, 74, 244, 531, 609
BstSFI	CTRYAG	1 cut(s)	54
BstSLI	GKGCMC	3 cut(s)	550, 654, 1094
BstUI	CGCG	4 cut(s)	213, 413, 444, 511
BstV1I	GCAGC	4 cut(s)	146, 173, 518, 732
BstX2I	RGATCY	1 cut(s)	308
BstYI	RGATCY	1 cut(s)	308
BstZI	CGGCCG	2 cut(s)	208, 534
BsuRI	GGCC	9 cut(s)	18, 44, 210, 536, 548, 595, 1135, 1145, 1256
BtgI	CCRYGG	1 cut(s)	590
BtsCI	GGATG	2 cut(s)	342, 560
Cac8I	GCNNGC	1 cut(s)	254
CfoI	GCGC	3 cut(s)	164, 413, 511
Cfr13I	GGNCC	5 cut(s)	9, 546, 547, 607, 1255
CseI	GACGC	1 cut(s)	450
Csp6I	GTAC	8 cut(s)	21, 178, 238, 343, 365, 558, 625, 867
CspCI	CAANNNNNGTGG	2 cut(s)	572, 607
CviAII	CATG	5 cut(s)	350, 458, 493, 818, 1223
CviQI	GTAC	8 cut(s)	21, 178, 238, 343, 365, 558, 625, 867
DpnI	GATC	6 cut(s)	310, 456, 632, 816, 1122, 1245
DpnII	GATC	6 cut(s)	308, 454, 630, 814, 1120, 1243
DriI	GACNNNNNGTC	1 cut(s)	670
EaeI	YGGCCR	3 cut(s)	42, 208, 534
EagI	CGGCCG	2 cut(s)	208, 534
Eam1104I	CTCTTC	3 cut(s)	399, 704, 768
Eam1105I	GACNNNNNGTC	1 cut(s)	670
EarI	CTCTTC	3 cut(s)	399, 704, 768
EciI	GGCGGA	1 cut(s)	437
EclXI	CGGCCG	2 cut(s)	208, 534
Eco130I	CCWWGG	1 cut(s)	550
Eco147I	AGGCCT	1 cut(s)	1145
Eco24I	GRGCYC	1 cut(s)	550
Eco31I	GGTCTC	1 cut(s)	708
Eco47I	GGWCC	2 cut(s)	9, 607
Eco52I	CGGCCG	2 cut(s)	208, 534
Eco57I	CTGAAG	1 cut(s)	110
Eco88I	CYCGRG	3 cut(s)	233, 653, 767
EcoNI	CCTNNNNNAGG	1 cut(s)	316
EcoO109I	RGGNCCY	2 cut(s)	547, 1255
EcoRII	CCWGG	1 cut(s)	59
EcoT14I	CCWWGG	1 cut(s)	550
EcoT38I	GRGCYC	1 cut(s)	550
ErhI	CCWWGG	1 cut(s)	550
Esp3I	CGTCTC	2 cut(s)	55, 917
FaeI	CATG	5 cut(s)	353, 461, 496, 821, 1226
FaqI	GGGAC	3 cut(s)	572, 783, 842
FatI	CATG	5 cut(s)	349, 457, 492, 817, 1222
FauI	CCCGC	1 cut(s)	275
FbaI	TGATCA	1 cut(s)	454
Fnu4HI	GCNGC	6 cut(s)	160, 187, 211, 250, 507, 746
FokI	GGATG	2 cut(s)	349, 567
FriOI	GRGCYC	1 cut(s)	550
Fsp4HI	GCNGC	6 cut(s)	160, 187, 211, 250, 507, 746
FspBI	CTAG	6 cut(s)	551, 738, 885, 1062, 1068, 1259
GlaI	GCGC	3 cut(s)	163, 412, 510
GluI	GCNGC	6 cut(s)	160, 187, 211, 250, 507, 746
HaeIII	GGCC	9 cut(s)	18, 44, 210, 536, 548, 595, 1135, 1145, 1256
HapII	CCGG	6 cut(s)	75, 245, 500, 533, 611, 628
HgaI	GACGC	1 cut(s)	450
HhaI	GCGC	3 cut(s)	164, 413, 511
Hin1I	GRCGYC	1 cut(s)	929
Hin1II	CATG	5 cut(s)	353, 461, 496, 821, 1226
Hin6I	GCGC	3 cut(s)	162, 411, 509
HinP1I	GCGC	3 cut(s)	162, 411, 509
HinfI	GANTC	5 cut(s)	71, 296, 658, 665, 779
HpaII	CCGG	6 cut(s)	75, 245, 500, 533, 611, 628
HphI	GGTGA	6 cut(s)	103, 409, 464, 656, 988, 994
Hpy166II	GTNNAC	2 cut(s)	150, 300
Hpy188I	TCNGA	3 cut(s)	262, 607, 1125
Hpy188III	TCNNGA	4 cut(s)	568, 662, 767, 1028
Hpy8I	GTNNAC	2 cut(s)	150, 300
Hpy99I	CGWCG	3 cut(s)	444, 931, 934
HpyAV	CCTTC	4 cut(s)	134, 267, 905, 1135
HpyCH4III	ACNGT	3 cut(s)	49, 808, 982
HpyCH4IV	ACGT	1 cut(s)	929
HpyCH4V	TGCA	3 cut(s)	189, 340, 1222
HpyF10VI	GCNNNNNNNGC	4 cut(s)	419, 503, 517, 751
HpySE526I	ACGT	1 cut(s)	929
Hsp92I	GRCGYC	1 cut(s)	929
Hsp92II	CATG	5 cut(s)	353, 461, 496, 821, 1226
HspAI	GCGC	3 cut(s)	162, 411, 509
Ksp22I	TGATCA	1 cut(s)	454
Kzo9I	GATC	6 cut(s)	308, 454, 630, 814, 1120, 1243
LmnI	GCTCC	3 cut(s)	41, 133, 467
Lsp1109I	GCAGC	4 cut(s)	146, 173, 518, 732
LweI	GCATC	2 cut(s)	327, 1009
MaeI	CTAG	6 cut(s)	551, 738, 885, 1062, 1068, 1259
MaeII	ACGT	1 cut(s)	929
MaeIII	GTNAC	4 cut(s)	371, 383, 397, 976
MalI	GATC	6 cut(s)	310, 456, 632, 816, 1122, 1245
MboI	GATC	6 cut(s)	308, 454, 630, 814, 1120, 1243
MboII	GAAGA	6 cut(s)	416, 721, 755, 785, 1028, 1049
MfeI	CAATTG	1 cut(s)	952
MflI	RGATCY	1 cut(s)	308
MhlI	GDGCHC	4 cut(s)	439, 550, 654, 1094
MluCI	AATT	9 cut(s)	360, 689, 877, 894, 952, 1057, 1150, 1205, 1264
MluI	ACGCGT	1 cut(s)	442
MlyI	GAGTC	2 cut(s)	305, 659
MmeI	TCCRAC	1 cut(s)	792
MseI	TTAA	7 cut(s)	290, 378, 1002, 1233, 1251, 1263, 1267
MslI	CAYNNNNRTG	2 cut(s)	354, 806
MspI	CCGG	6 cut(s)	75, 245, 500, 533, 611, 628
MspR9I	CCNGG	5 cut(s)	61, 76, 246, 533, 611
MunI	CAATTG	1 cut(s)	952
Mva1269I	GAATGC	3 cut(s)	754, 922, 951
MvaI	CCWGG	1 cut(s)	61
MvnI	CGCG	4 cut(s)	213, 413, 444, 511
MwoI	GCNNNNNNNGC	4 cut(s)	419, 503, 517, 751
NciI	CCSGG	4 cut(s)	76, 246, 533, 611
NdeII	GATC	6 cut(s)	308, 454, 630, 814, 1120, 1243
NlaIII	CATG	5 cut(s)	353, 461, 496, 821, 1226
NlaIV	GGNNCC	6 cut(s)	37, 463, 498, 530, 548, 609
NmeAIII	GCCGAG	2 cut(s)	20, 946
NmuCI	GTSAC	4 cut(s)	371, 383, 397, 976
NspI	RCATGY	1 cut(s)	353
NspV	TTCGAA	1 cut(s)	271
OliI	CACNNNNGTG	1 cut(s)	806
PacI	TTAATTAA	1 cut(s)	1267
PaeR7I	CTCGAG	2 cut(s)	233, 767
PceI	AGGCCT	1 cut(s)	1145
PctI	GAATGC	3 cut(s)	754, 922, 951
PfeI	GAWTC	3 cut(s)	71, 658, 779
PflFI	GACNNNGTC	1 cut(s)	928
PkrI	GCNGC	6 cut(s)	161, 188, 212, 251, 508, 747
PleI	GAGTC	2 cut(s)	304, 659
PpsI	GAGTC	2 cut(s)	304, 659
Psp6I	CCWGG	1 cut(s)	59
PspGI	CCWGG	1 cut(s)	59
PspN4I	GGNNCC	6 cut(s)	37, 463, 498, 530, 548, 609
PspOMI	GGGCCC	1 cut(s)	546
PspPI	GGNCC	5 cut(s)	9, 546, 547, 607, 1255
PspXI	VCTCGAGB	1 cut(s)	233
PsuI	RGATCY	1 cut(s)	308
PsyI	GACNNNGTC	1 cut(s)	928
RsaI	GTAC	8 cut(s)	22, 179, 239, 344, 366, 559, 626, 868
RsaNI	GTAC	8 cut(s)	21, 178, 238, 343, 365, 558, 625, 867
RseI	CAYNNNNRTG	2 cut(s)	354, 806
SaqAI	TTAA	7 cut(s)	290, 378, 1002, 1233, 1251, 1263, 1267
SatI	GCNGC	6 cut(s)	160, 187, 211, 250, 507, 746
Sau3AI	GATC	6 cut(s)	308, 454, 630, 814, 1120, 1243
Sau96I	GGNCC	5 cut(s)	9, 546, 547, 607, 1255
SchI	GAGTC	2 cut(s)	305, 659
ScrFI	CCNGG	5 cut(s)	61, 76, 246, 533, 611
SduI	GDGCHC	4 cut(s)	439, 550, 654, 1094
SfaNI	GCATC	2 cut(s)	327, 1009
SfcI	CTRYAG	1 cut(s)	54
Sfr274I	CTCGAG	2 cut(s)	233, 767
SfuI	TTCGAA	1 cut(s)	271
SinI	GGWCC	2 cut(s)	9, 607
SlaI	CTCGAG	2 cut(s)	233, 767
SmiMI	CAYNNNNRTG	2 cut(s)	354, 806
SmlI	CTYRAG	3 cut(s)	233, 749, 767
SmoI	CTYRAG	3 cut(s)	233, 749, 767
Sse9I	AATT	9 cut(s)	360, 689, 877, 894, 952, 1057, 1150, 1205, 1264
SseBI	AGGCCT	1 cut(s)	1145
SsiI	CCGC	5 cut(s)	211, 249, 282, 422, 465
SspI	AATATT	1 cut(s)	391
SspMI	CTAG	6 cut(s)	551, 738, 885, 1062, 1068, 1259
StuI	AGGCCT	1 cut(s)	1145
StyD4I	CCNGG	5 cut(s)	59, 74, 244, 531, 609
StyI	CCWWGG	1 cut(s)	550
TaaI	ACNGT	3 cut(s)	49, 808, 982
TaiI	ACGT	1 cut(s)	932
TaqI	TCGA	6 cut(s)	234, 271, 439, 567, 768, 1242
TasI	AATT	9 cut(s)	360, 689, 877, 894, 952, 1057, 1150, 1205, 1264
TatI	WGTACW	2 cut(s)	364, 557
TauI	GCSGC	2 cut(s)	213, 252
TfiI	GAWTC	3 cut(s)	71, 658, 779
Tru1I	TTAA	7 cut(s)	290, 378, 1002, 1233, 1251, 1263, 1267
Tru9I	TTAA	7 cut(s)	290, 378, 1002, 1233, 1251, 1263, 1267
TseFI	GTSAC	4 cut(s)	371, 383, 397, 976
TseI	GCWGC	4 cut(s)	159, 186, 506, 745
Tsp45I	GTSAC	4 cut(s)	371, 383, 397, 976
TspDTI	ATGAA	3 cut(s)	17, 279, 1050
TspGWI	ACGGA	1 cut(s)	416
Tth111I	GACNNNGTC	1 cut(s)	928
VpaK11BI	GGWCC	2 cut(s)	9, 607
XagI	CCTNNNNNAGG	1 cut(s)	316
XapI	RAATTY	5 cut(s)	689, 877, 1057, 1150, 1205
XceI	RCATGY	1 cut(s)	353
XcmI	CCANNNNNNNNNTGG	2 cut(s)	726, 808
XhoI	CTCGAG	2 cut(s)	233, 767
XmaJI	CCTAGG	1 cut(s)	550
XspI	CTAG	6 cut(s)	551, 738, 885, 1062, 1068, 1259
ZraI	GACGTC	1 cut(s)	930

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.