Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0005142

Physical Location & Seq

Genomic Coordinates Reverse (-)

46855755 .. 46859688

Gene Structure 3934 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1351700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1635 bp

ATGAACAAGAAAGGCTTGGCAATTTTATTGCGTACCAGAATGCAGCGCCCAAGCTCCGATCCCAGAGACCTCGCCCGCTCTCCTCTCGCCAACGTGGTTAACCAAATGCTGCCTAATTCCGATGGAAGACAATCGAGCGGACGACTGGTGCCGCATGGAAGGGAAAAGCTCAATGCCTCGACCAACACCGAGAGGGAATTTGAGGTTGTGCGGGAGTCCATGCACTCGGCGATTTCACTGAGCAAAACCGAAATTTTAGACTCTGTACTGAGCGATTTCTCGGAGGGATATTTTAGCCTCTCTTACGAAAATCGTCGAAAGCTACTTGTACTACTTGCCAAAGAGTATGATCTTAACAGGACGCAAGTTCGCGATTTGATCAAGCAATATTTGGGACTTGAGCTCCCTGAAAGTGACAGTGCTCCCTCAACTGGCTCTGAAGAGGAGACATCTCTTTCTGCTTTCTATCGTATTGAGCGCAATCTGAGACATGCTCTCAAGCCGATGTACGAAGTTCTGTTTGAGCGACTCAACACACATCCTGGCGGGTTGAAGTTCTTGTCCAATCTTCGAGCTGATATTCTATCTATTATCGCAGAGGAAAATACTGCATCTTTGCGAGCACTGGACTCCTACTTAAAGGAGAAACTAAGTACATGGCTTAGTCCTGCTGCCTTAGAGCTTCACCAAATCACATGGGACGATCCTGCCTCTTTACTGGAAAAGATTGTGGCATATGAGGCTGTGCATCCAATCAGCAATCTTATAGATCTTAAGAGAAGGCTGGGAGTCGGTCGCCGTTGTTTCGGATATTTACATCCAGCAATACCTGGTGAGCCCCTTATTTTCATCGAAGTTGCACTCATGAAGGATGTGGCTCAAACAGTACAGGAAGTTTTATGGGATGACCCTCCTACTCCCGAATGTGAGGCTACGTGTGCATTGTTTTACTCCATATCATCAACCCAGCCTGGCTTATCAGGGATCAACCTGGGAAGGTTTCTAATTAAACGTGTGATCACACTTGTGAAAAGAGAAATGACAAATATTTCTACTTTTGCGACTCTCAGCCCCATCCCAGGTTTCATGCAGTGGCTCCTATCTAAGCTGGCTTCTCAATCAAAACTTGCTGAAGGTGAAGCAATGCCACACTCACCAGCTTCTGATAGGTCCAGTTCTACGTTTTCGGAGAAAATTCTTGAACCAGAAGAGGAAAGAGCACTTATGGATGCTTCTGCGGAATTCACTTCTGGGAAAAATAGCATGGACGTGATGTTCAACTTGCTAACTTCACCAAATCATGAGTGGACAAATTCTGATAAATTGCTTTCAGTATTAAAACCCCCTCTAATGCGACTGTGTGCCAGGTACCTTCTCCAAGAGAAAAAGAGAGGAAAAGCTCTGGATTCTGTTGCGAATTTTCACTTACAGAATGGAGCTATGGTCGAAAGAATCAATTGGATGGGGGACCGGTCAGGGAGAGGCCTCCATCAAAGTGGAGGTATGATGGTGAATTATGTTTACAGATTGGGGCAAATTGAAGACTATGCTCAGTCATATTTCAGTACAGGGCTTATCCATACTTCTAGTGATCTTCGCCGTTACGTTGAGACAATTAGTCGTGTACAAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006084 GO:0006085 GO:0006139 GO:0006163 GO:0006164 GO:0006195 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009150 GO:0009152 GO:0009154 GO:0009165 GO:0009166 GO:0009259 GO:0009260 GO:0009261 GO:0009893 GO:0009987 GO:0010565 GO:0016829 GO:0016830 GO:0016831 GO:0018130 GO:0019216 GO:0019217 GO:0019222 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0031323 GO:0031325 GO:0031974 GO:0033865 GO:0033866 GO:0033869 GO:0033875 GO:0034030 GO:0034031 GO:0034032 GO:0034033 GO:0034034 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0035383 GO:0035384 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044272 GO:0044273 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045834 GO:0045923 GO:0046320 GO:0046321 GO:0046390 GO:0046434 GO:0046483 GO:0046700 GO:0048518 GO:0048522 GO:0050080 GO:0050789 GO:0050794 GO:0051186 GO:0051188 GO:0055086 GO:0062012 GO:0062013 GO:0065007 GO:0070013 GO:0071616 GO:0071704 GO:0072521 GO:0072522 GO:0072523 GO:0080090 GO:0090407 GO:1901135 GO:1901136 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:2001293 GO:2001294

KEGG Pathways

Metabolic & Signaling

ko00410 ko00640 ko01100 ko04146 ko04152 map00410 map00640 map01100 map04146 map04152

Pfam Domains

Protein Families

MCD MCD_N

Protein Analysis

545

Amino Acids

61.27

Weight (kDa)

6.5

Isoelectric Point (pI)

49.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCD_N	PF17408	157 - 220	8.9e-12	Malonyl-CoA decarboxylase N-terminal domain
MCD	PF05292	225 - 508	1.3e-90	Malonyl-CoA decarboxylase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005142)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04320 AT4G04320
fragaria_vesca	FvH4_3g01470 FvH4_3g01470 FvH4_3g01470 FvH4_3g01470 FvH4_3g01470 FvH4_3g01470
malus_domestica	MD05G1351700.v1.1
prunus_persica	Prupe.4G015900_v2.0.a1 Prupe.4G015900_v2.0.a1 Prupe.I000100_v2.0.a1 Prupe.I000100_v2.0.a1 Prupe.I000100_v2.0.a1 Prupe.I000100_v2.0.a1
pyrus_communis	pycom05g31880
rosa_chinensis	RchiOBHm_Chr3g0487171 RchiOBHm_Chr5g0002321
rosa_laevigata	RLG00000031019
rosa_multiflora	Rmu_sc0003879.1_g000001 Rmu_sc0006137.1_g000008
rosa_roxburghii	Rroxscaffold_1G00073550
rosa_rugosa	Rorug04G0395300
rosa_samantha	Rh3AG279900 Rh5AG018000 Rh5BG020600 Rh5BG020700 Rh5CG019900 Rh5DG018700
rosa_wichuraiana	Rw5G001690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1368
AccB1I	GGYRCC	2 cut(s)	148, 1368
AccBSI	CCGCTC	2 cut(s)	78, 138
AccII	CGCG	1 cut(s)	372
AciI	CCGC	6 cut(s)	76, 138, 152, 211, 546, 1238
AclWI	GGATC	3 cut(s)	53, 698, 992
AcsI	RAATTY	6 cut(s)	197, 252, 1194, 1241, 1312, 1417
AcuI	CTGAAG	2 cut(s)	459, 1152
AfaI	GTAC	9 cut(s)	34, 267, 330, 509, 655, 888, 1370, 1567, 1626
AfiI	CCNNNNNNNGG	2 cut(s)	431, 1079
AflII	CTTAAG	1 cut(s)	773
AflIII	ACRYGT	2 cut(s)	935, 1012
AgeI	ACCGGT	1 cut(s)	1470
AgsI	TTSAA	4 cut(s)	553, 1202, 1279, 1541
AhdI	GACNNNNNGTC	1 cut(s)	1617
AjiI	CACGTC	1 cut(s)	1270
AjnI	CCWGG	6 cut(s)	541, 829, 970, 990, 1078, 1364
AleI	CACNNNNGTG	1 cut(s)	1025
AloI	GAACNNNNNNTCC	2 cut(s)	1259, 1291
Alw21I	GWGCWC	4 cut(s)	405, 424, 625, 1222
Alw26I	GTCTC	4 cut(s)	60, 440, 481, 1604
AlwI	GGATC	3 cut(s)	53, 698, 992
AlwNI	CAGNNNCTG	1 cut(s)	1163
AoxI	GGCC	1 cut(s)	1483
ApeKI	GCWGC	3 cut(s)	43, 109, 671
ApoI	RAATTY	6 cut(s)	197, 252, 1194, 1241, 1312, 1417
AsiGI	ACCGGT	1 cut(s)	1470
Asp718I	GGTACC	1 cut(s)	1368
AspLEI	GCGC	2 cut(s)	48, 480
AspS9I	GGNCC	2 cut(s)	1170, 1468
AsuHPI	GGTGA	6 cut(s)	677, 845, 1146, 1148, 1284, 1522
AvaII	GGWCC	2 cut(s)	1170, 1468
BanI	GGYRCC	2 cut(s)	148, 1368
BanII	GRGCYC	2 cut(s)	405, 840
BbsI	GAAGAC	2 cut(s)	133, 1548
Bbv12I	GWGCWC	4 cut(s)	405, 424, 625, 1222
BbvI	GCAGC	3 cut(s)	55, 96, 658
BccI	CCATC	5 cut(s)	116, 1082, 1456, 1497, 1501
BceAI	ACGGC	2 cut(s)	783, 1584
BciT130I	CCWGG	6 cut(s)	543, 831, 972, 992, 1080, 1366
BclI	TGATCA	2 cut(s)	378, 1017
BcoDI	GTCTC	4 cut(s)	60, 440, 481, 1604
BfaI	CTAG	1 cut(s)	1587
BfoI	RGCGCY	1 cut(s)	49
BfrI	CTTAAG	1 cut(s)	773
BglII	AGATCT	1 cut(s)	769
BisI	GCNGC	4 cut(s)	44, 110, 152, 672
BlsI	GCNGC	4 cut(s)	45, 111, 153, 673
Bme1390I	CCNGG	6 cut(s)	543, 831, 972, 992, 1080, 1366
Bme18I	GGWCC	2 cut(s)	1170, 1468
BmeRI	GACNNNNNGTC	1 cut(s)	1617
BmgBI	CACGTC	1 cut(s)	1270
BmgT120I	GGNCC	2 cut(s)	1170, 1468
BmiI	GGNNCC	4 cut(s)	150, 1097, 1370, 1469
BmrFI	CCNGG	6 cut(s)	543, 831, 972, 992, 1080, 1366
BmsI	GCATC	3 cut(s)	620, 757, 1219
BpiI	GAAGAC	2 cut(s)	133, 1548
BplI	GAGNNNNNCTC	2 cut(s)	478, 510
BpuEI	CTTGAG	2 cut(s)	419, 482
BsaAI	YACGTR	1 cut(s)	936
BsaI	GGTCTC	1 cut(s)	60
BsaJI	CCNNGG	2 cut(s)	991, 1078
BsaWI	WCCGGW	1 cut(s)	1470
BsaXI	ACNNNNNCTCC	4 cut(s)	387, 417, 1428, 1458
Bsc4I	CCNNNNNNNGG	2 cut(s)	431, 1079
Bse118I	RCCGGY	1 cut(s)	1470
Bse1I	ACTGG	5 cut(s)	150, 436, 630, 723, 1173
Bse3DI	GCAATG	1 cut(s)	1149
BseBI	CCWGG	6 cut(s)	543, 831, 972, 992, 1080, 1366
BseDI	CCNNGG	2 cut(s)	991, 1078
BseGI	GGATG	8 cut(s)	538, 748, 817, 877, 910, 1074, 1234, 1467
BseLI	CCNNNNNNNGG	2 cut(s)	431, 1079
BseMI	GCAATG	1 cut(s)	1149
BseMII	CTCAG	5 cut(s)	230, 260, 476, 1081, 1565
BseNI	ACTGG	5 cut(s)	150, 436, 630, 723, 1173
BseRI	GAGGAG	2 cut(s)	72, 458
BseXI	GCAGC	3 cut(s)	55, 96, 658
BseYI	CCCAGC	2 cut(s)	784, 966
Bsh1236I	CGCG	1 cut(s)	372
Bsh1285I	CGRYCG	1 cut(s)	796
BshFI	GGCC	1 cut(s)	1485
BshNI	GGYRCC	2 cut(s)	148, 1368
BshTI	ACCGGT	1 cut(s)	1470
BsiEI	CGRYCG	1 cut(s)	796
BsiHKAI	GWGCWC	4 cut(s)	405, 424, 625, 1222
BsiSI	CCGG	1 cut(s)	1471
BslFI	GGGAC	3 cut(s)	408, 713, 1481
BslI	CCNNNNNNNGG	2 cut(s)	431, 1079
BsmAI	GTCTC	4 cut(s)	60, 440, 481, 1604
BsmFI	GGGAC	3 cut(s)	408, 713, 1481
BsmI	GAATGC	1 cut(s)	45
BsnI	GGCC	1 cut(s)	1485
Bso31I	GGTCTC	1 cut(s)	60
Bsp1286I	GDGCHC	5 cut(s)	405, 424, 625, 840, 1222
Bsp1407I	TGTACA	1 cut(s)	1624
Bsp143I	GATC	8 cut(s)	58, 349, 378, 703, 769, 984, 1017, 1591
Bsp68I	TCGCGA	1 cut(s)	372
BspACI	CCGC	6 cut(s)	76, 138, 152, 211, 546, 1238
BspANI	GGCC	1 cut(s)	1485
BspCNI	CTCAG	5 cut(s)	231, 261, 477, 1080, 1564
BspFNI	CGCG	1 cut(s)	372
BspHI	TCATGA	2 cut(s)	864, 1300
BspLI	GGNNCC	4 cut(s)	150, 1097, 1370, 1469
BspPI	GGATC	3 cut(s)	53, 698, 992
BspT107I	GGYRCC	2 cut(s)	148, 1368
BspTI	CTTAAG	1 cut(s)	773
BspTNI	GGTCTC	1 cut(s)	60
BsrBI	CCGCTC	2 cut(s)	78, 138
BsrDI	GCAATG	1 cut(s)	1149
BsrFI	RCCGGY	1 cut(s)	1470
BsrGI	TGTACA	1 cut(s)	1624
BsrI	ACTGG	5 cut(s)	150, 436, 630, 723, 1173
BssAI	RCCGGY	1 cut(s)	1470
BssECI	CCNNGG	2 cut(s)	991, 1078
BssMI	GATC	8 cut(s)	58, 349, 378, 703, 769, 984, 1017, 1591
Bst2UI	CCWGG	6 cut(s)	543, 831, 972, 992, 1080, 1366
Bst4CI	ACNGT	3 cut(s)	419, 886, 1359
Bst6I	CTCTTC	2 cut(s)	435, 1203
BstAFI	CTTAAG	1 cut(s)	773
BstAUI	TGTACA	1 cut(s)	1624
BstBAI	YACGTR	1 cut(s)	936
BstC8I	GCNNGC	3 cut(s)	76, 621, 1110
BstDEI	CTNAG	9 cut(s)	239, 269, 485, 650, 662, 676, 1067, 1104, 1551
BstF5I	GGATG	8 cut(s)	538, 748, 817, 877, 910, 1074, 1234, 1467
BstFNI	CGCG	1 cut(s)	372
BstH2I	RGCGCY	1 cut(s)	49
BstHHI	GCGC	2 cut(s)	48, 480
BstKTI	GATC	8 cut(s)	61, 352, 381, 706, 772, 987, 1020, 1594
BstMAI	GTCTC	4 cut(s)	60, 440, 481, 1604
BstMBI	GATC	8 cut(s)	58, 349, 378, 703, 769, 984, 1017, 1591
BstMCI	CGRYCG	1 cut(s)	796
BstMWI	GCNNNNNNNGC	2 cut(s)	740, 938
BstNI	CCWGG	6 cut(s)	543, 831, 972, 992, 1080, 1366
BstNSI	RCATGY	1 cut(s)	494
BstSCI	CCNGG	6 cut(s)	541, 829, 970, 990, 1078, 1364
BstUI	CGCG	1 cut(s)	372
BstV1I	GCAGC	3 cut(s)	55, 96, 658
BstV2I	GAAGAC	2 cut(s)	133, 1548
BstX2I	RGATCY	1 cut(s)	769
BstXI	CCANNNNNNTGG	1 cut(s)	1496
BstYI	RGATCY	1 cut(s)	769
BsuRI	GGCC	1 cut(s)	1485
BtrI	CACGTC	1 cut(s)	1270
BtsCI	GGATG	8 cut(s)	538, 748, 817, 877, 910, 1074, 1234, 1467
BtsI	GCAGTG	1 cut(s)	1097
BtsIMutI	CAGTG	4 cut(s)	236, 424, 623, 1097
BtuMI	TCGCGA	1 cut(s)	372
Cac8I	GCNNGC	3 cut(s)	76, 621, 1110
CaiI	CAGNNNCTG	1 cut(s)	1163
CciI	TCATGA	2 cut(s)	864, 1300
CfoI	GCGC	2 cut(s)	48, 480
Cfr10I	RCCGGY	1 cut(s)	1470
Cfr13I	GGNCC	2 cut(s)	1170, 1468
CseI	GACGC	1 cut(s)	370
CsiI	ACCWGGT	1 cut(s)	829
Csp6I	GTAC	9 cut(s)	33, 266, 329, 508, 654, 887, 1369, 1566, 1625
CspAI	ACCGGT	1 cut(s)	1470
CviAII	CATG	9 cut(s)	155, 220, 491, 657, 696, 865, 1087, 1264, 1301
CviQI	GTAC	9 cut(s)	33, 266, 329, 508, 654, 887, 1369, 1566, 1625
DdeI	CTNAG	9 cut(s)	239, 269, 485, 650, 662, 676, 1067, 1104, 1551
DpnI	GATC	8 cut(s)	60, 351, 380, 705, 771, 986, 1019, 1593
DpnII	GATC	8 cut(s)	58, 349, 378, 703, 769, 984, 1017, 1591
DriI	GACNNNNNGTC	1 cut(s)	1617
Eam1104I	CTCTTC	2 cut(s)	435, 1203
Eam1105I	GACNNNNNGTC	1 cut(s)	1617
EarI	CTCTTC	2 cut(s)	435, 1203
Ecl136II	GAGCTC	1 cut(s)	403
Eco147I	AGGCCT	1 cut(s)	1485
Eco24I	GRGCYC	2 cut(s)	405, 840
Eco31I	GGTCTC	1 cut(s)	60
Eco47I	GGWCC	2 cut(s)	1170, 1468
Eco53kI	GAGCTC	1 cut(s)	403
Eco57I	CTGAAG	2 cut(s)	459, 1152
EcoICRI	GAGCTC	1 cut(s)	403
EcoRI	GAATTC	1 cut(s)	1241
EcoRII	CCWGG	6 cut(s)	541, 829, 970, 990, 1078, 1364
EcoT38I	GRGCYC	2 cut(s)	405, 840
FaeI	CATG	9 cut(s)	158, 223, 494, 660, 699, 868, 1090, 1267, 1304
FalI	AAGNNNNNCTT	1 cut(s)	31
FaqI	GGGAC	3 cut(s)	408, 713, 1481
FatI	CATG	9 cut(s)	154, 219, 490, 656, 695, 864, 1086, 1263, 1300
FauI	CCCGC	3 cut(s)	83, 204, 539
FauNDI	CATATG	1 cut(s)	736
FbaI	TGATCA	2 cut(s)	378, 1017
Fnu4HI	GCNGC	4 cut(s)	44, 110, 152, 672
FokI	GGATG	8 cut(s)	525, 735, 804, 884, 917, 1061, 1241, 1474
FriOI	GRGCYC	2 cut(s)	405, 840
Fsp4HI	GCNGC	4 cut(s)	44, 110, 152, 672
FspBI	CTAG	1 cut(s)	1587
GlaI	GCGC	2 cut(s)	47, 479
GluI	GCNGC	4 cut(s)	44, 110, 152, 672
GsaI	CCCAGC	2 cut(s)	788, 970
HaeII	RGCGCY	1 cut(s)	49
HaeIII	GGCC	1 cut(s)	1485
HapII	CCGG	1 cut(s)	1471
HgaI	GACGC	1 cut(s)	370
HhaI	GCGC	2 cut(s)	48, 480
Hin1II	CATG	9 cut(s)	158, 223, 494, 660, 699, 868, 1090, 1267, 1304
Hin6I	GCGC	2 cut(s)	46, 478
HinP1I	GCGC	2 cut(s)	46, 478
HincII	GTYRAC	1 cut(s)	100
HindII	GTYRAC	1 cut(s)	100
HinfI	GANTC	8 cut(s)	215, 260, 528, 629, 789, 1063, 1406, 1452
HpaI	GTTAAC	1 cut(s)	100
HpaII	CCGG	1 cut(s)	1471
HphI	GGTGA	6 cut(s)	677, 845, 1146, 1148, 1284, 1522
Hpy166II	GTNNAC	4 cut(s)	100, 1308, 1522, 1625
Hpy188I	TCNGA	9 cut(s)	58, 121, 283, 439, 486, 809, 1165, 1189, 1318
Hpy188III	TCNNGA	6 cut(s)	371, 865, 920, 1199, 1301, 1403
Hpy8I	GTNNAC	4 cut(s)	100, 1308, 1522, 1625
Hpy99I	CGWCG	1 cut(s)	318
HpyAV	CCTTC	6 cut(s)	153, 774, 862, 990, 1127, 1382
HpyCH4III	ACNGT	3 cut(s)	419, 886, 1359
HpyCH4IV	ACGT	6 cut(s)	93, 935, 1012, 1181, 1269, 1605
HpyCH4V	TGCA	7 cut(s)	43, 223, 611, 748, 860, 941, 1090
HpyF10VI	GCNNNNNNNGC	2 cut(s)	740, 938
HpyF3I	CTNAG	9 cut(s)	239, 269, 485, 650, 662, 676, 1067, 1104, 1551
HpySE526I	ACGT	6 cut(s)	93, 935, 1012, 1181, 1269, 1605
Hsp92II	CATG	9 cut(s)	158, 223, 494, 660, 699, 868, 1090, 1267, 1304
HspAI	GCGC	2 cut(s)	46, 478
KpnI	GGTACC	1 cut(s)	1372
Ksp22I	TGATCA	2 cut(s)	378, 1017
KspAI	GTTAAC	1 cut(s)	100
Kzo9I	GATC	8 cut(s)	58, 349, 378, 703, 769, 984, 1017, 1591
LmnI	GCTCC	5 cut(s)	59, 408, 427, 1101, 1436
Lsp1109I	GCAGC	3 cut(s)	55, 96, 658
LweI	GCATC	3 cut(s)	620, 757, 1219
MabI	ACCWGGT	1 cut(s)	829
MaeI	CTAG	1 cut(s)	1587
MaeII	ACGT	6 cut(s)	93, 935, 1012, 1181, 1269, 1605
MaeIII	GTNAC	2 cut(s)	413, 1601
MalI	GATC	8 cut(s)	60, 351, 380, 705, 771, 986, 1019, 1593
MbiI	CCGCTC	2 cut(s)	78, 138
MboI	GATC	8 cut(s)	58, 349, 378, 703, 769, 984, 1017, 1591
MboII	GAAGA	6 cut(s)	138, 452, 560, 1220, 1553, 1586
MfeI	CAATTG	1 cut(s)	1456
MflI	RGATCY	1 cut(s)	769
MhlI	GDGCHC	5 cut(s)	405, 424, 625, 840, 1222
MlyI	GAGTC	6 cut(s)	224, 254, 522, 623, 798, 1057
MseI	TTAA	6 cut(s)	99, 354, 638, 774, 1008, 1337
MslI	CAYNNNNRTG	3 cut(s)	1025, 1268, 1494
MspCI	CTTAAG	1 cut(s)	773
MspI	CCGG	1 cut(s)	1471
MspR9I	CCNGG	6 cut(s)	543, 831, 972, 992, 1080, 1366
MunI	CAATTG	1 cut(s)	1456
Mva1269I	GAATGC	1 cut(s)	45
MvaI	CCWGG	6 cut(s)	543, 831, 972, 992, 1080, 1366
MvnI	CGCG	1 cut(s)	372
MwoI	GCNNNNNNNGC	2 cut(s)	740, 938
NdeI	CATATG	1 cut(s)	736
NdeII	GATC	8 cut(s)	58, 349, 378, 703, 769, 984, 1017, 1591
NlaIII	CATG	9 cut(s)	158, 223, 494, 660, 699, 868, 1090, 1267, 1304
NlaIV	GGNNCC	4 cut(s)	150, 1097, 1370, 1469
NmeAIII	GCCGAG	1 cut(s)	206
NmuCI	GTSAC	1 cut(s)	413
NruI	TCGCGA	1 cut(s)	372
NspI	RCATGY	1 cut(s)	494
OliI	CACNNNNGTG	1 cut(s)	1025
PagI	TCATGA	2 cut(s)	864, 1300
PceI	AGGCCT	1 cut(s)	1485
PctI	GAATGC	1 cut(s)	45
PfeI	GAWTC	2 cut(s)	1406, 1452
PinAI	ACCGGT	1 cut(s)	1470
PkrI	GCNGC	4 cut(s)	45, 111, 153, 673
PleI	GAGTC	6 cut(s)	223, 254, 522, 623, 797, 1057
PpsI	GAGTC	6 cut(s)	223, 254, 522, 623, 797, 1057
Ppu21I	YACGTR	1 cut(s)	936
Psp124BI	GAGCTC	1 cut(s)	405
Psp6I	CCWGG	6 cut(s)	541, 829, 970, 990, 1078, 1364
PspFI	CCCAGC	2 cut(s)	784, 966
PspGI	CCWGG	6 cut(s)	541, 829, 970, 990, 1078, 1364
PspN4I	GGNNCC	4 cut(s)	150, 1097, 1370, 1469
PspPI	GGNCC	2 cut(s)	1170, 1468
PstNI	CAGNNNCTG	1 cut(s)	1163
PsuI	RGATCY	1 cut(s)	769
RruI	TCGCGA	1 cut(s)	372
RsaI	GTAC	9 cut(s)	34, 267, 330, 509, 655, 888, 1370, 1567, 1626
RsaNI	GTAC	9 cut(s)	33, 266, 329, 508, 654, 887, 1369, 1566, 1625
RseI	CAYNNNNRTG	3 cut(s)	1025, 1268, 1494
SacI	GAGCTC	1 cut(s)	405
SaqAI	TTAA	6 cut(s)	99, 354, 638, 774, 1008, 1337
SatI	GCNGC	4 cut(s)	44, 110, 152, 672
Sau3AI	GATC	8 cut(s)	58, 349, 378, 703, 769, 984, 1017, 1591
Sau96I	GGNCC	2 cut(s)	1170, 1468
SchI	GAGTC	6 cut(s)	224, 254, 522, 623, 798, 1057
ScrFI	CCNGG	6 cut(s)	543, 831, 972, 992, 1080, 1366
SduI	GDGCHC	5 cut(s)	405, 424, 625, 840, 1222
SexAI	ACCWGGT	1 cut(s)	829
SfaNI	GCATC	3 cut(s)	620, 757, 1219
SinI	GGWCC	2 cut(s)	1170, 1468
SmiMI	CAYNNNNRTG	3 cut(s)	1025, 1268, 1494
SmlI	CTYRAG	3 cut(s)	398, 497, 773
SmoI	CTYRAG	3 cut(s)	398, 497, 773
SseBI	AGGCCT	1 cut(s)	1485
SsiI	CCGC	6 cut(s)	76, 138, 152, 211, 546, 1238
SspI	AATATT	2 cut(s)	389, 1048
SspMI	CTAG	1 cut(s)	1587
SstI	GAGCTC	1 cut(s)	405
StuI	AGGCCT	1 cut(s)	1485
StyD4I	CCNGG	6 cut(s)	541, 829, 970, 990, 1078, 1364
TaaI	ACNGT	3 cut(s)	419, 886, 1359
TaiI	ACGT	6 cut(s)	96, 938, 1015, 1184, 1272, 1608
TaqI	TCGA	6 cut(s)	134, 179, 316, 571, 852, 1446
TaqII	GACCGA	1 cut(s)	782
TatI	WGTACW	6 cut(s)	265, 328, 653, 886, 1565, 1624
TauI	GCSGC	1 cut(s)	154
TfiI	GAWTC	2 cut(s)	1406, 1452
Tru1I	TTAA	6 cut(s)	99, 354, 638, 774, 1008, 1337
Tru9I	TTAA	6 cut(s)	99, 354, 638, 774, 1008, 1337
TscAI	CASTG	4 cut(s)	243, 424, 630, 1097
TseFI	GTSAC	1 cut(s)	413
TseI	GCWGC	3 cut(s)	43, 109, 671
Tsp45I	GTSAC	1 cut(s)	413
TspDTI	ATGAA	4 cut(s)	17, 838, 881, 1075
TspRI	CASTG	4 cut(s)	243, 424, 630, 1097
Vha464I	CTTAAG	1 cut(s)	773
VpaK11BI	GGWCC	2 cut(s)	1170, 1468
XapI	RAATTY	6 cut(s)	197, 252, 1194, 1241, 1312, 1417
XceI	RCATGY	1 cut(s)	494
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1302
XspI	CTAG	1 cut(s)	1587

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.