pycom05g31880

Malonyl-CoA decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005142
Physical Location & Seq
Reverse (-)
31430903 .. 31434590
3688 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g31880.1

Sequence Viewer

Length: 1602 bp
ATGAGGCGGCACCAGATGGCAGATGCGATAGAAAAATGCGAAGACAGGGAGAGAGAACTCGACCTTCGAACGTTGTTAACCAAACGCTGCCTAATTCCGATGGAGGACGATCGAGCGGACGACTGGTGCAGCATGGAAGCGAAAAGCTCAATGCCTCGACCAACACCGAGAGTTGTGCGGGAGTCCATGCACTCGGCGATTTCACTGAGCAAAACCGAAATTTTAGACTCTGTACTGAACGATTTCTCGGGGGGATATTTTAGCCTCTCTTACGAAAATCGTCGAAAGCTACTTGTACTACTTGCCAAAGAGTATGATCTTAACAGGACGCAAGTTCGCGATTTGATCAAGCAATATTTGGGACTTGAGCTCCCTGAAAGTGACAGTGCTCCCTCAACTGGCTCCGAAGAGGAGACATCTCTTTCTGCTTTCTACCGTATTGAGCGCAATCTGAGACATGCTCTCAAGCCGATGTACGAAGTTCTGTTTGAGCGACTCAACACACATCCTGGCGGGTTGAAGTTCTTGTCCAATCTTCGAGCTGATATTCTATCTATTATCGCAGAGGAAAATACTGCATCTTTGCGAGCACTGGACTCCTACTTAAAGGAGAAACTAAGTACATGGCTTAGTCCTGCTGCCTTAGAGCTTCACCAAATCACATGGGACGATCCTGCCTCTTTACTGGAAAAGATTGTGGCATATGAGGCTGTGCATCCAATCAGCAATCTTATAGATCTTAAGAGAAGGCTGGGAGTCGGTCGCCGTTGTTTTGGATATTTACATCCAGCAATACCTGGCGAGCCCCTTATTTTCATCGAAGTTGCACTCATGAAGGATGTGGCTCAAACAGTACAGGAAGTTTTATGGGATGACCCTCCTACTCCCGAATGTGAGGCTACATGTGCATTGTTTTACTCCATATCATCAACTCAGCCTGGCTTATCAGGGATCAACCTGGGAAGGTTTCTAATTAAACGTGTGATCACACTTGTGAAAAGAGAAATGACAAATATTTCTACTTTTGCGACTCTCAGCCCCATCCCAGGTTTCATGCAGTGGCTCCTATCTAAGCTGGCTTCTCAATCAAAACTTGCTGAAGGTGAAGCAATGCCACACTCACCAGCTTCTGATAGGTCCAGTTTTACGTTTTCGGAGAAAATTCTTGAACCAGAAGAGGAAAGAGCACTTATGGATGCTTCTGCGGAATTCAATTCTGGGAAAAATAGCATGGACGTGATGTTCAACTTGCTGACTTCACCAAATCATGAGTGGACAAATTCTGATAAATTGCTTTCAGTATTAAAACCCCCTCTAATGCGACTGTGTGCCAGGTACCTTCTCCAAGAGAAAAAGAGAGGAAAAGCTCTGGATTCTGTTGCGAATTTTCACTTACAGAATGGAGCTATGGTCGAAAGAATCAATTGGATGGGGGACCGGTCAGAGAAAGGCCTCCATCAAAGTGGAGGTATGATGGTGAATTATGTTTACAGACTGGGGCAAATTGAAGACTATGCTCAGTCATATTTCAGTACAGGGCTTATCCATACTTCTAGTGATCTTCGCCGTTACGTTGAGACAATTAGTCGTGTACAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006084 GO:0006085 GO:0006139 GO:0006163 GO:0006164 GO:0006195 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009150 GO:0009152 GO:0009154 GO:0009165 GO:0009166 GO:0009259 GO:0009260 GO:0009261 GO:0009893 GO:0009987 GO:0010565 GO:0016829 GO:0016830 GO:0016831 GO:0018130 GO:0019216 GO:0019217 GO:0019222 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0031323 GO:0031325 GO:0031974 GO:0033865 GO:0033866 GO:0033869 GO:0033875 GO:0034030 GO:0034031 GO:0034032 GO:0034033 GO:0034034 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0035383 GO:0035384 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044272 GO:0044273 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045834 GO:0045923 GO:0046320 GO:0046321 GO:0046390 GO:0046434 GO:0046483 GO:0046700 GO:0048518 GO:0048522 GO:0050080 GO:0050789 GO:0050794 GO:0051186 GO:0051188 GO:0055086 GO:0062012 GO:0062013 GO:0065007 GO:0070013 GO:0071616 GO:0071704 GO:0072521 GO:0072522 GO:0072523 GO:0080090 GO:0090407 GO:1901135 GO:1901136 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:2001293 GO:2001294
KEGG Pathways
Metabolic & Signaling
ko00410 ko00640 ko01100 ko04146 ko04152 map00410 map00640 map01100 map04146 map04152
Pfam Domains
Protein Families
MCD MCD_N

Protein Analysis

534

Amino Acids

60.54

Weight (kDa)

5.85

Isoelectric Point (pI)

49.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCD_N PF17408 146 - 209 8.6e-12 Malonyl-CoA decarboxylase N-terminal domain
MCD PF05292 214 - 497 2.8e-91 Malonyl-CoA decarboxylase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1335
AccB1I GGYRCC 2 cut(s) 9, 1335
AccBSI CCGCTC 1 cut(s) 116
AccII CGCG 1 cut(s) 339
AciI CCGC 5 cut(s) 7, 116, 178, 513, 1205
AclI AACGTT 1 cut(s) 71
AclWI GGATC 2 cut(s) 665, 959
AcsI RAATTY 5 cut(s) 219, 1161, 1208, 1279, 1384
AcuI CTGAAG 1 cut(s) 1119
AfaI GTAC 8 cut(s) 234, 297, 476, 622, 855, 1337, 1534, 1593
AfiI CCNNNNNNNGG 2 cut(s) 398, 1046
AflII CTTAAG 1 cut(s) 740
AflIII ACRYGT 2 cut(s) 902, 979
AgeI ACCGGT 1 cut(s) 1437
AgsI TTSAA 5 cut(s) 520, 1169, 1213, 1246, 1508
AhdI GACNNNNNGTC 1 cut(s) 1584
AjiI CACGTC 1 cut(s) 1237
AjnI CCWGG 6 cut(s) 508, 796, 937, 957, 1045, 1331
AleI CACNNNNGTG 1 cut(s) 992
AloI GAACNNNNNNTCC 2 cut(s) 1226, 1258
AluBI AGCT 9 cut(s) 147, 289, 370, 542, 649, 1075, 1127, 1367, 1406
AluI AGCT 9 cut(s) 147, 289, 370, 542, 649, 1075, 1127, 1367, 1406
Alw21I GWGCWC 4 cut(s) 372, 391, 592, 1189
Alw26I GTCTC 3 cut(s) 407, 448, 1571
AlwI GGATC 2 cut(s) 665, 959
AlwNI CAGNNNCTG 1 cut(s) 1130
Ama87I CYCGRG 1 cut(s) 247
AoxI GGCC 1 cut(s) 1450
ApeKI GCWGC 3 cut(s) 87, 129, 638
ApoI RAATTY 5 cut(s) 219, 1161, 1208, 1279, 1384
AsiGI ACCGGT 1 cut(s) 1437
Asp700I GAANNNNTTC 1 cut(s) 242
Asp718I GGTACC 1 cut(s) 1335
AspLEI GCGC 1 cut(s) 447
AspS9I GGNCC 2 cut(s) 1137, 1435
AsuHPI GGTGA 5 cut(s) 644, 1113, 1115, 1251, 1489
AsuII TTCGAA 1 cut(s) 67
AvaI CYCGRG 1 cut(s) 247
AvaII GGWCC 2 cut(s) 1137, 1435
BanI GGYRCC 2 cut(s) 9, 1335
BanII GRGCYC 2 cut(s) 372, 807
BbsI GAAGAC 2 cut(s) 48, 1515
Bbv12I GWGCWC 4 cut(s) 372, 391, 592, 1189
BbvI GCAGC 3 cut(s) 74, 141, 625
BccI CCATC 6 cut(s) 10, 94, 1049, 1423, 1464, 1468
BceAI ACGGC 2 cut(s) 750, 1551
BcgI CGANNNNNNTGC 2 cut(s) 157, 191
BciT130I CCWGG 6 cut(s) 510, 798, 939, 959, 1047, 1333
BclI TGATCA 2 cut(s) 345, 984
BcoDI GTCTC 3 cut(s) 407, 448, 1571
BfaI CTAG 1 cut(s) 1554
BfrI CTTAAG 1 cut(s) 740
BglII AGATCT 1 cut(s) 736
BisI GCNGC 4 cut(s) 8, 88, 130, 639
BlsI GCNGC 4 cut(s) 9, 89, 131, 640
Bme1390I CCNGG 6 cut(s) 510, 798, 939, 959, 1047, 1333
Bme18I GGWCC 2 cut(s) 1137, 1435
BmeRI GACNNNNNGTC 1 cut(s) 1584
BmeT110I CYCGRG 1 cut(s) 247
BmgBI CACGTC 1 cut(s) 1237
BmgT120I GGNCC 2 cut(s) 1137, 1435
BmiI GGNNCC 5 cut(s) 11, 403, 1064, 1337, 1436
BmrFI CCNGG 6 cut(s) 510, 798, 939, 959, 1047, 1333
BmrI ACTGGG 1 cut(s) 1505
BmsI GCATC 4 cut(s) 13, 587, 724, 1186
BmuI ACTGGG 1 cut(s) 1505
BpiI GAAGAC 2 cut(s) 48, 1515
BplI GAGNNNNNCTC 2 cut(s) 445, 477
Bpu14I TTCGAA 1 cut(s) 67
BpuEI CTTGAG 2 cut(s) 386, 449
BsaJI CCNNGG 2 cut(s) 958, 1045
BsaWI WCCGGW 1 cut(s) 1437
BsaXI ACNNNNNCTCC 4 cut(s) 354, 384, 1395, 1425
Bsc4I CCNNNNNNNGG 2 cut(s) 398, 1046
Bse118I RCCGGY 1 cut(s) 1437
Bse1I ACTGG 6 cut(s) 128, 403, 597, 690, 1140, 1500
Bse3DI GCAATG 1 cut(s) 1116
BseBI CCWGG 6 cut(s) 510, 798, 939, 959, 1047, 1333
BseDI CCNNGG 2 cut(s) 958, 1045
BseGI GGATG 8 cut(s) 505, 715, 784, 844, 877, 1041, 1201, 1434
BseLI CCNNNNNNNGG 2 cut(s) 398, 1046
BseMI GCAATG 1 cut(s) 1116
BseMII CTCAG 5 cut(s) 197, 443, 947, 1048, 1532
BseNI ACTGG 6 cut(s) 128, 403, 597, 690, 1140, 1500
BseRI GAGGAG 1 cut(s) 425
BseXI GCAGC 3 cut(s) 74, 141, 625
BseYI CCCAGC 1 cut(s) 751
BsgI GTGCAG 1 cut(s) 148
Bsh1236I CGCG 1 cut(s) 339
Bsh1285I CGRYCG 2 cut(s) 112, 763
BshFI GGCC 1 cut(s) 1452
BshNI GGYRCC 2 cut(s) 9, 1335
BshTI ACCGGT 1 cut(s) 1437
BsiEI CGRYCG 2 cut(s) 112, 763
BsiHKAI GWGCWC 4 cut(s) 372, 391, 592, 1189
BsiHKCI CYCGRG 1 cut(s) 247
BsiSI CCGG 1 cut(s) 1438
BslFI GGGAC 3 cut(s) 375, 680, 1448
BslI CCNNNNNNNGG 2 cut(s) 398, 1046
BsmAI GTCTC 3 cut(s) 407, 448, 1571
BsmFI GGGAC 3 cut(s) 375, 680, 1448
BsnI GGCC 1 cut(s) 1452
BsoBI CYCGRG 1 cut(s) 247
Bsp119I TTCGAA 1 cut(s) 67
Bsp1286I GDGCHC 5 cut(s) 372, 391, 592, 807, 1189
Bsp1407I TGTACA 1 cut(s) 1591
Bsp143I GATC 8 cut(s) 109, 316, 345, 670, 736, 951, 984, 1558
Bsp68I TCGCGA 1 cut(s) 339
BspACI CCGC 5 cut(s) 7, 116, 178, 513, 1205
BspANI GGCC 1 cut(s) 1452
BspCNI CTCAG 5 cut(s) 198, 444, 946, 1047, 1531
BspFNI CGCG 1 cut(s) 339
BspHI TCATGA 2 cut(s) 831, 1267
BspLI GGNNCC 5 cut(s) 11, 403, 1064, 1337, 1436
BspPI GGATC 2 cut(s) 665, 959
BspT104I TTCGAA 1 cut(s) 67
BspT107I GGYRCC 2 cut(s) 9, 1335
BspTI CTTAAG 1 cut(s) 740
BsrBI CCGCTC 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 1116
BsrFI RCCGGY 1 cut(s) 1437
BsrGI TGTACA 1 cut(s) 1591
BsrI ACTGG 6 cut(s) 128, 403, 597, 690, 1140, 1500
BssAI RCCGGY 1 cut(s) 1437
BssECI CCNNGG 2 cut(s) 958, 1045
BssMI GATC 8 cut(s) 109, 316, 345, 670, 736, 951, 984, 1558
Bst2UI CCWGG 6 cut(s) 510, 798, 939, 959, 1047, 1333
Bst4CI ACNGT 4 cut(s) 386, 437, 853, 1326
Bst6I CTCTTC 2 cut(s) 402, 1170
BstAFI CTTAAG 1 cut(s) 740
BstAUI TGTACA 1 cut(s) 1591
BstBI TTCGAA 1 cut(s) 67
BstC8I GCNNGC 3 cut(s) 588, 803, 1077
BstDEI CTNAG 9 cut(s) 206, 452, 617, 629, 643, 933, 1034, 1071, 1518
BstF5I GGATG 8 cut(s) 505, 715, 784, 844, 877, 1041, 1201, 1434
BstFNI CGCG 1 cut(s) 339
BstHHI GCGC 1 cut(s) 447
BstKTI GATC 8 cut(s) 112, 319, 348, 673, 739, 954, 987, 1561
BstMAI GTCTC 3 cut(s) 407, 448, 1571
BstMBI GATC 8 cut(s) 109, 316, 345, 670, 736, 951, 984, 1558
BstMCI CGRYCG 2 cut(s) 112, 763
BstMWI GCNNNNNNNGC 2 cut(s) 707, 905
BstNI CCWGG 6 cut(s) 510, 798, 939, 959, 1047, 1333
BstNSI RCATGY 2 cut(s) 461, 906
BstSCI CCNGG 6 cut(s) 508, 796, 937, 957, 1045, 1331
BstUI CGCG 1 cut(s) 339
BstV1I GCAGC 3 cut(s) 74, 141, 625
BstV2I GAAGAC 2 cut(s) 48, 1515
BstX2I RGATCY 1 cut(s) 736
BstXI CCANNNNNNTGG 1 cut(s) 1463
BstYI RGATCY 1 cut(s) 736
BsuRI GGCC 1 cut(s) 1452
BtrI CACGTC 1 cut(s) 1237
BtsCI GGATG 8 cut(s) 505, 715, 784, 844, 877, 1041, 1201, 1434
BtsI GCAGTG 1 cut(s) 1064
BtsIMutI CAGTG 4 cut(s) 203, 391, 590, 1064
BtuMI TCGCGA 1 cut(s) 339
Cac8I GCNNGC 3 cut(s) 588, 803, 1077
CaiI CAGNNNCTG 1 cut(s) 1130
CciI TCATGA 2 cut(s) 831, 1267
CfoI GCGC 1 cut(s) 447
Cfr10I RCCGGY 1 cut(s) 1437
Cfr13I GGNCC 2 cut(s) 1137, 1435
CseI GACGC 1 cut(s) 337
Csp6I GTAC 8 cut(s) 233, 296, 475, 621, 854, 1336, 1533, 1592
CspAI ACCGGT 1 cut(s) 1437
CviQI GTAC 8 cut(s) 233, 296, 475, 621, 854, 1336, 1533, 1592
DdeI CTNAG 9 cut(s) 206, 452, 617, 629, 643, 933, 1034, 1071, 1518
DpnI GATC 8 cut(s) 111, 318, 347, 672, 738, 953, 986, 1560
DpnII GATC 8 cut(s) 109, 316, 345, 670, 736, 951, 984, 1558
DriI GACNNNNNGTC 1 cut(s) 1584
Eam1104I CTCTTC 2 cut(s) 402, 1170
Eam1105I GACNNNNNGTC 1 cut(s) 1584
EarI CTCTTC 2 cut(s) 402, 1170
Ecl136II GAGCTC 1 cut(s) 370
Eco147I AGGCCT 1 cut(s) 1452
Eco24I GRGCYC 2 cut(s) 372, 807
Eco47I GGWCC 2 cut(s) 1137, 1435
Eco53kI GAGCTC 1 cut(s) 370
Eco57I CTGAAG 1 cut(s) 1119
Eco88I CYCGRG 1 cut(s) 247
EcoICRI GAGCTC 1 cut(s) 370
EcoRI GAATTC 1 cut(s) 1208
EcoRII CCWGG 6 cut(s) 508, 796, 937, 957, 1045, 1331
EcoT38I GRGCYC 2 cut(s) 372, 807
FaqI GGGAC 3 cut(s) 375, 680, 1448
FauI CCCGC 2 cut(s) 171, 506
FauNDI CATATG 1 cut(s) 703
FbaI TGATCA 2 cut(s) 345, 984
Fnu4HI GCNGC 4 cut(s) 8, 88, 130, 639
FokI GGATG 8 cut(s) 492, 702, 771, 851, 884, 1028, 1208, 1441
FriOI GRGCYC 2 cut(s) 372, 807
Fsp4HI GCNGC 4 cut(s) 8, 88, 130, 639
FspBI CTAG 1 cut(s) 1554
GlaI GCGC 1 cut(s) 446
GluI GCNGC 4 cut(s) 8, 88, 130, 639
GsaI CCCAGC 1 cut(s) 755
HaeIII GGCC 1 cut(s) 1452
HapII CCGG 1 cut(s) 1438
HgaI GACGC 1 cut(s) 337
HhaI GCGC 1 cut(s) 447
Hin6I GCGC 1 cut(s) 445
HinP1I GCGC 1 cut(s) 445
HincII GTYRAC 1 cut(s) 78
HindII GTYRAC 1 cut(s) 78
HinfI GANTC 8 cut(s) 182, 227, 495, 596, 756, 1030, 1373, 1419
HpaI GTTAAC 1 cut(s) 78
HpaII CCGG 1 cut(s) 1438
HphI GGTGA 5 cut(s) 644, 1113, 1115, 1251, 1489
Hpy166II GTNNAC 4 cut(s) 78, 1275, 1489, 1592
Hpy188I TCNGA 7 cut(s) 99, 406, 453, 1132, 1156, 1285, 1444
Hpy188III TCNNGA 6 cut(s) 338, 832, 887, 1166, 1268, 1370
Hpy8I GTNNAC 4 cut(s) 78, 1275, 1489, 1592
Hpy99I CGWCG 1 cut(s) 285
HpyAV CCTTC 6 cut(s) 74, 741, 829, 957, 1094, 1349
HpyCH4III ACNGT 4 cut(s) 386, 437, 853, 1326
HpyCH4IV ACGT 5 cut(s) 71, 979, 1148, 1236, 1572
HpyCH4V TGCA 7 cut(s) 129, 190, 578, 715, 827, 908, 1057
HpyF10VI GCNNNNNNNGC 2 cut(s) 707, 905
HpyF3I CTNAG 9 cut(s) 206, 452, 617, 629, 643, 933, 1034, 1071, 1518
HpySE526I ACGT 5 cut(s) 71, 979, 1148, 1236, 1572
HspAI GCGC 1 cut(s) 445
KpnI GGTACC 1 cut(s) 1339
Ksp22I TGATCA 2 cut(s) 345, 984
KspAI GTTAAC 1 cut(s) 78
Kzo9I GATC 8 cut(s) 109, 316, 345, 670, 736, 951, 984, 1558
LmnI GCTCC 5 cut(s) 375, 394, 407, 1068, 1403
Lsp1109I GCAGC 3 cut(s) 74, 141, 625
LweI GCATC 4 cut(s) 13, 587, 724, 1186
MaeI CTAG 1 cut(s) 1554
MaeII ACGT 5 cut(s) 71, 979, 1148, 1236, 1572
MaeIII GTNAC 2 cut(s) 380, 1568
MalI GATC 8 cut(s) 111, 318, 347, 672, 738, 953, 986, 1560
MbiI CCGCTC 1 cut(s) 116
MboI GATC 8 cut(s) 109, 316, 345, 670, 736, 951, 984, 1558
MboII GAAGA 6 cut(s) 53, 419, 527, 1187, 1520, 1553
MfeI CAATTG 1 cut(s) 1423
MflI RGATCY 1 cut(s) 736
MhlI GDGCHC 5 cut(s) 372, 391, 592, 807, 1189
MlyI GAGTC 6 cut(s) 191, 221, 489, 590, 765, 1024
MroXI GAANNNNTTC 1 cut(s) 242
MseI TTAA 6 cut(s) 77, 321, 605, 741, 975, 1304
MslI CAYNNNNRTG 3 cut(s) 992, 1235, 1461
MspCI CTTAAG 1 cut(s) 740
MspI CCGG 1 cut(s) 1438
MspR9I CCNGG 6 cut(s) 510, 798, 939, 959, 1047, 1333
MunI CAATTG 1 cut(s) 1423
MvaI CCWGG 6 cut(s) 510, 798, 939, 959, 1047, 1333
MvnI CGCG 1 cut(s) 339
MwoI GCNNNNNNNGC 2 cut(s) 707, 905
NdeI CATATG 1 cut(s) 703
NdeII GATC 8 cut(s) 109, 316, 345, 670, 736, 951, 984, 1558
NlaIV GGNNCC 5 cut(s) 11, 403, 1064, 1337, 1436
NmeAIII GCCGAG 1 cut(s) 173
NmuCI GTSAC 1 cut(s) 380
NruI TCGCGA 1 cut(s) 339
NspI RCATGY 2 cut(s) 461, 906
NspV TTCGAA 1 cut(s) 67
OliI CACNNNNGTG 1 cut(s) 992
PagI TCATGA 2 cut(s) 831, 1267
PceI AGGCCT 1 cut(s) 1452
PciI ACATGT 1 cut(s) 902
PdmI GAANNNNTTC 1 cut(s) 242
PfeI GAWTC 2 cut(s) 1373, 1419
PinAI ACCGGT 1 cut(s) 1437
PkrI GCNGC 4 cut(s) 9, 89, 131, 640
Ple19I CGATCG 1 cut(s) 112
PleI GAGTC 6 cut(s) 190, 221, 489, 590, 764, 1024
PpsI GAGTC 6 cut(s) 190, 221, 489, 590, 764, 1024
PscI ACATGT 1 cut(s) 902
Psp124BI GAGCTC 1 cut(s) 372
Psp1406I AACGTT 1 cut(s) 71
Psp6I CCWGG 6 cut(s) 508, 796, 937, 957, 1045, 1331
PspFI CCCAGC 1 cut(s) 751
PspGI CCWGG 6 cut(s) 508, 796, 937, 957, 1045, 1331
PspN4I GGNNCC 5 cut(s) 11, 403, 1064, 1337, 1436
PspPI GGNCC 2 cut(s) 1137, 1435
PstNI CAGNNNCTG 1 cut(s) 1130
PsuI RGATCY 1 cut(s) 736
PvuI CGATCG 1 cut(s) 112
RruI TCGCGA 1 cut(s) 339
RsaI GTAC 8 cut(s) 234, 297, 476, 622, 855, 1337, 1534, 1593
RsaNI GTAC 8 cut(s) 233, 296, 475, 621, 854, 1336, 1533, 1592
RseI CAYNNNNRTG 3 cut(s) 992, 1235, 1461
SacI GAGCTC 1 cut(s) 372
SaqAI TTAA 6 cut(s) 77, 321, 605, 741, 975, 1304
SatI GCNGC 4 cut(s) 8, 88, 130, 639
Sau3AI GATC 8 cut(s) 109, 316, 345, 670, 736, 951, 984, 1558
Sau96I GGNCC 2 cut(s) 1137, 1435
SchI GAGTC 6 cut(s) 191, 221, 489, 590, 765, 1024
ScrFI CCNGG 6 cut(s) 510, 798, 939, 959, 1047, 1333
SduI GDGCHC 5 cut(s) 372, 391, 592, 807, 1189
SfaNI GCATC 4 cut(s) 13, 587, 724, 1186
SfuI TTCGAA 1 cut(s) 67
SinI GGWCC 2 cut(s) 1137, 1435
SmiMI CAYNNNNRTG 3 cut(s) 992, 1235, 1461
SmlI CTYRAG 3 cut(s) 365, 464, 740
SmoI CTYRAG 3 cut(s) 365, 464, 740
SseBI AGGCCT 1 cut(s) 1452
SsiI CCGC 5 cut(s) 7, 116, 178, 513, 1205
SspI AATATT 2 cut(s) 356, 1015
SspMI CTAG 1 cut(s) 1554
SstI GAGCTC 1 cut(s) 372
StuI AGGCCT 1 cut(s) 1452
StyD4I CCNGG 6 cut(s) 508, 796, 937, 957, 1045, 1331
TaaI ACNGT 4 cut(s) 386, 437, 853, 1326
TaiI ACGT 5 cut(s) 74, 982, 1151, 1239, 1575
TaqI TCGA 8 cut(s) 60, 67, 112, 157, 283, 538, 819, 1413
TaqII GACCGA 1 cut(s) 749
TatI WGTACW 6 cut(s) 232, 295, 620, 853, 1532, 1591
TauI GCSGC 1 cut(s) 10
TfiI GAWTC 2 cut(s) 1373, 1419
Tru1I TTAA 6 cut(s) 77, 321, 605, 741, 975, 1304
Tru9I TTAA 6 cut(s) 77, 321, 605, 741, 975, 1304
TscAI CASTG 4 cut(s) 210, 391, 597, 1064
TseFI GTSAC 1 cut(s) 380
TseI GCWGC 3 cut(s) 87, 129, 638
Tsp45I GTSAC 1 cut(s) 380
TspDTI ATGAA 3 cut(s) 805, 848, 1042
TspRI CASTG 4 cut(s) 210, 391, 597, 1064
Vha464I CTTAAG 1 cut(s) 740
VpaK11BI GGWCC 2 cut(s) 1137, 1435
XapI RAATTY 5 cut(s) 219, 1161, 1208, 1279, 1384
XceI RCATGY 2 cut(s) 461, 906
XcmI CCANNNNNNNNNTGG 1 cut(s) 1269
XmnI GAANNNNTTC 1 cut(s) 242
XspI CTAG 1 cut(s) 1554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.