Rh5BG020600

Malonyl-CoA decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0005142
Physical Location & Seq
Forward (+)
1534035 .. 1536800
2766 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG020600.1

Sequence Viewer

Length: 1203 bp
ATGAACAAGAAAGGTTTGGCAATTCTAATGCGAACCAGAATGAGACCCACCAACAACGATTCAGCTCACCTCGCTCGCTCTCCTCTCGCCAACATGGTTAATCAAACGGTGAATGGAGGCCAATCAAGTAGTGGAACCCAAAAGCTCAATGCTTTAAGCAACACCAAGAGGGATTTTGAGGTTGTAAGGGAGTCCATGCACTCTGCAATTTCAATGAGTAAAACTGAAGTCTTGGATTCCGTGCTGAATGATTTCTCAGAGGGATATTTCAGCCTTTCGTATGAGAATCGTCGAAAATTGCTTGTGCAACTTGCCAAAGAGTATGATGTTAATAGGACACAAGTTCGCGATTTGATAAAGCAGTATTTGGGACTTGAGCCTCCTGGAACTGGAAGTGACAGTGCTCACTCAGTTGGCGTTGAAGAGGAGGCATCTCTTTCTGCATTCTACCGCATTGAGAGGAACTTGAGACATGCTCTCAAGCCGATGTATGAAGTGCTATTTGAGCGGCTTAATACGCATCCCGGAGGGTTGAAGTTCTTGTCCATTCTTCGAGCTGATATTTTATCAATTCTCACAGAAGACAATCTTGCGTCTTTGCGAGCACTGGATTCCTATTTAAAGGAGAAACTTAGTATGTGGCTTAGTCCTGCTGCCTTAGAGCTCCACCAGATCACATGGAATGATCCTGCCTCTTTGCTGGAAAAAATTGTGGCTTATGAGGCCGTGCACCCAATCAGCAATCTTATAGATCTTAAGAGAAGGTTGGGAGTTGGTCGCCGGTGTTTTGGATACTTTCATCCAGCAATACCTGGTGAACCCCTTATTTTCATAGAAGTCGCACTCATGAAAGATATGGCTCAATCTGTACAGGAAGTTCTATGGGATGACCCTCCAATTCCTGAATGTGAGGCTACATGTGCATTGTTTTACTCCATATCATCAACTCAGCCTGGCTTGTCGGGGATAAACCTGGGAAAGTTTCTAATCAAACGTGTGATCACACTGGTGAGGAAAGACATGGCACATATTTCTACATTTGCGACTCTCAGCCCCATTCCAGGGTATATGCAATGGCTCCTATCAAAATTGGCATCCCAGTCAAAACTTGCTGAAGGTGAAGACATGCCAAACTCGTCAGCTAGACCTGTAAATGGACCGGATTTGGATCGGATCGACCTAAATCCAAATCCGTTTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006084 GO:0006085 GO:0006139 GO:0006163 GO:0006164 GO:0006195 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009150 GO:0009152 GO:0009154 GO:0009165 GO:0009166 GO:0009259 GO:0009260 GO:0009261 GO:0009893 GO:0009987 GO:0010565 GO:0016829 GO:0016830 GO:0016831 GO:0018130 GO:0019216 GO:0019217 GO:0019222 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0031323 GO:0031325 GO:0031974 GO:0033865 GO:0033866 GO:0033869 GO:0033875 GO:0034030 GO:0034031 GO:0034032 GO:0034033 GO:0034034 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0035383 GO:0035384 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044272 GO:0044273 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045834 GO:0045923 GO:0046320 GO:0046321 GO:0046390 GO:0046434 GO:0046483 GO:0046700 GO:0048518 GO:0048522 GO:0050080 GO:0050789 GO:0050794 GO:0051186 GO:0051188 GO:0055086 GO:0062012 GO:0062013 GO:0065007 GO:0070013 GO:0071616 GO:0071704 GO:0072521 GO:0072522 GO:0072523 GO:0080090 GO:0090407 GO:1901135 GO:1901136 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:2001293 GO:2001294
KEGG Pathways
Metabolic & Signaling
ko00410 ko00640 ko01100 ko04146 ko04152 map00410 map00640 map01100 map04146 map04152
Pfam Domains
Protein Families
MCD MCD_N

Protein Analysis

400

Amino Acids

44.67

Weight (kDa)

6.77

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCD_N PF17408 151 - 214 4e-11 Malonyl-CoA decarboxylase N-terminal domain
MCD PF05292 219 - 372 5.9e-61 Malonyl-CoA decarboxylase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 508
AccII CGCG 1 cut(s) 348
AciI CCGC 2 cut(s) 451, 508
AclWI GGATC 3 cut(s) 680, 1176, 1181
AcuI CTGAAG 2 cut(s) 246, 1134
AfaI GTAC 1 cut(s) 870
AfiI CCNNNNNNNGG 4 cut(s) 389, 1061, 1062, 1154
AflII CTTAAG 1 cut(s) 755
AflIII ACRYGT 2 cut(s) 917, 994
AgsI TTSAA 3 cut(s) 213, 422, 535
AjnI CCWGG 5 cut(s) 382, 811, 952, 972, 1060
AleI CACNNNNGTG 1 cut(s) 1007
AluBI AGCT 5 cut(s) 65, 145, 557, 664, 1142
AluI AGCT 5 cut(s) 65, 145, 557, 664, 1142
Alw21I GWGCWC 4 cut(s) 406, 607, 666, 732
Alw26I GTCTC 2 cut(s) 37, 463
Alw44I GTGCAC 1 cut(s) 728
AlwI GGATC 3 cut(s) 680, 1176, 1181
AoxI GGCC 2 cut(s) 118, 723
ApaLI GTGCAC 1 cut(s) 728
ApeKI GCWGC 1 cut(s) 653
Asp700I GAANNNNTTC 1 cut(s) 251
AspS9I GGNCC 1 cut(s) 1157
AsuC2I CCSGG 1 cut(s) 525
AsuHPI GGTGA 5 cut(s) 59, 121, 827, 1021, 1130
AvaII GGWCC 1 cut(s) 1157
BaeGI GKGCMC 1 cut(s) 732
BanII GRGCYC 1 cut(s) 666
BbsI GAAGAC 2 cut(s) 588, 1128
Bbv12I GWGCWC 4 cut(s) 406, 607, 666, 732
BbvI GCAGC 1 cut(s) 640
BceAI ACGGC 1 cut(s) 710
BciT130I CCWGG 5 cut(s) 384, 813, 954, 974, 1062
BciVI GTATCC 1 cut(s) 785
BclI TGATCA 1 cut(s) 999
BcnI CCSGG 1 cut(s) 525
BcoDI GTCTC 2 cut(s) 37, 463
BfaI CTAG 1 cut(s) 1143
BfrI CTTAAG 1 cut(s) 755
BfuI GTATCC 1 cut(s) 785
BglII AGATCT 1 cut(s) 751
BisI GCNGC 2 cut(s) 509, 654
BlsI GCNGC 2 cut(s) 510, 655
Bme1390I CCNGG 6 cut(s) 384, 525, 813, 954, 974, 1062
Bme18I GGWCC 1 cut(s) 1157
BmgT120I GGNCC 1 cut(s) 1157
BmiI GGNNCC 2 cut(s) 136, 1079
BmrFI CCNGG 6 cut(s) 384, 525, 813, 954, 974, 1062
BmrI ACTGGG 1 cut(s) 1093
BmsI GCATC 3 cut(s) 440, 529, 1103
BmuI ACTGGG 1 cut(s) 1093
BpiI GAAGAC 2 cut(s) 588, 1128
BplI GAGNNNNNCTC 2 cut(s) 460, 492
BpuEI CTTGAG 3 cut(s) 395, 464, 487
BpuMI CCSGG 1 cut(s) 525
BsaBI GATNNNNATC 1 cut(s) 1167
BsaI GGTCTC 1 cut(s) 37
BsaJI CCNNGG 2 cut(s) 973, 1061
BsaWI WCCGGW 1 cut(s) 1159
Bsc4I CCNNNNNNNGG 4 cut(s) 389, 1061, 1062, 1154
Bse118I RCCGGY 1 cut(s) 780
Bse1I ACTGG 4 cut(s) 394, 612, 1011, 1099
Bse3DI GCAATG 1 cut(s) 1079
Bse8I GATNNNNATC 1 cut(s) 1167
BseBI CCWGG 5 cut(s) 384, 813, 954, 974, 1062
BseDI CCNNGG 2 cut(s) 973, 1061
BseGI GGATG 4 cut(s) 520, 799, 892, 1094
BseJI GATNNNNATC 1 cut(s) 1167
BseLI CCNNNNNNNGG 4 cut(s) 389, 1061, 1062, 1154
BseMI GCAATG 1 cut(s) 1079
BseMII CTCAG 4 cut(s) 270, 423, 962, 1063
BseNI ACTGG 4 cut(s) 394, 612, 1011, 1099
BseRI GAGGAG 2 cut(s) 72, 440
BseSI GKGCMC 1 cut(s) 732
BseXI GCAGC 1 cut(s) 640
Bsh1236I CGCG 1 cut(s) 348
BshFI GGCC 2 cut(s) 120, 725
BsiHKAI GWGCWC 4 cut(s) 406, 607, 666, 732
BsiSI CCGG 3 cut(s) 525, 781, 1160
BslFI GGGAC 1 cut(s) 384
BslI CCNNNNNNNGG 4 cut(s) 389, 1061, 1062, 1154
BsmAI GTCTC 2 cut(s) 37, 463
BsmFI GGGAC 1 cut(s) 384
BsmI GAATGC 1 cut(s) 443
BsnI GGCC 2 cut(s) 120, 725
Bso31I GGTCTC 1 cut(s) 37
Bsp1286I GDGCHC 4 cut(s) 406, 607, 666, 732
Bsp1407I TGTACA 1 cut(s) 868
Bsp143I GATC 6 cut(s) 672, 685, 751, 999, 1168, 1173
Bsp68I TCGCGA 1 cut(s) 348
BspACI CCGC 2 cut(s) 451, 508
BspANI GGCC 2 cut(s) 120, 725
BspCNI CTCAG 4 cut(s) 269, 422, 961, 1062
BspFNI CGCG 1 cut(s) 348
BspHI TCATGA 1 cut(s) 846
BspLI GGNNCC 2 cut(s) 136, 1079
BspPI GGATC 3 cut(s) 680, 1176, 1181
BspTI CTTAAG 1 cut(s) 755
BspTNI GGTCTC 1 cut(s) 37
BsrBI CCGCTC 1 cut(s) 508
BsrDI GCAATG 1 cut(s) 1079
BsrFI RCCGGY 1 cut(s) 780
BsrGI TGTACA 1 cut(s) 868
BsrI ACTGG 4 cut(s) 394, 612, 1011, 1099
BssAI RCCGGY 1 cut(s) 780
BssECI CCNNGG 2 cut(s) 973, 1061
BssMI GATC 6 cut(s) 672, 685, 751, 999, 1168, 1173
Bst2UI CCWGG 5 cut(s) 384, 813, 954, 974, 1062
Bst4CI ACNGT 2 cut(s) 109, 401
Bst6I CTCTTC 1 cut(s) 417
BstAFI CTTAAG 1 cut(s) 755
BstAUI TGTACA 1 cut(s) 868
BstC8I GCNNGC 2 cut(s) 76, 603
BstDEI CTNAG 7 cut(s) 256, 409, 632, 644, 658, 948, 1049
BstF5I GGATG 4 cut(s) 520, 799, 892, 1094
BstFNI CGCG 1 cut(s) 348
BstKTI GATC 6 cut(s) 675, 688, 754, 1002, 1171, 1176
BstMAI GTCTC 2 cut(s) 37, 463
BstMBI GATC 6 cut(s) 672, 685, 751, 999, 1168, 1173
BstMWI GCNNNNNNNGC 5 cut(s) 71, 505, 517, 722, 920
BstNI CCWGG 5 cut(s) 384, 813, 954, 974, 1062
BstNSI RCATGY 3 cut(s) 476, 921, 1129
BstSCI CCNGG 6 cut(s) 382, 523, 811, 952, 972, 1060
BstSLI GKGCMC 1 cut(s) 732
BstUI CGCG 1 cut(s) 348
BstV1I GCAGC 1 cut(s) 640
BstV2I GAAGAC 2 cut(s) 588, 1128
BstX2I RGATCY 1 cut(s) 751
BstYI RGATCY 1 cut(s) 751
BsuI GTATCC 1 cut(s) 785
BsuRI GGCC 2 cut(s) 120, 725
BtsCI GGATG 4 cut(s) 520, 799, 892, 1094
BtsIMutI CAGTG 3 cut(s) 406, 605, 1004
BtuMI TCGCGA 1 cut(s) 348
Cac8I GCNNGC 2 cut(s) 76, 603
CciI TCATGA 1 cut(s) 846
Cfr10I RCCGGY 1 cut(s) 780
Cfr13I GGNCC 1 cut(s) 1157
CseI GACGC 1 cut(s) 582
CsiI ACCWGGT 1 cut(s) 811
Csp6I GTAC 1 cut(s) 869
CviAII CATG 8 cut(s) 94, 196, 473, 678, 847, 918, 1021, 1126
CviQI GTAC 1 cut(s) 869
DdeI CTNAG 7 cut(s) 256, 409, 632, 644, 658, 948, 1049
DpnI GATC 6 cut(s) 674, 687, 753, 1001, 1170, 1175
DpnII GATC 6 cut(s) 672, 685, 751, 999, 1168, 1173
DraI TTTAAA 1 cut(s) 621
Eam1104I CTCTTC 1 cut(s) 417
EarI CTCTTC 1 cut(s) 417
Ecl136II GAGCTC 1 cut(s) 664
Eco24I GRGCYC 1 cut(s) 666
Eco31I GGTCTC 1 cut(s) 37
Eco47I GGWCC 1 cut(s) 1157
Eco53kI GAGCTC 1 cut(s) 664
Eco57I CTGAAG 2 cut(s) 246, 1134
EcoICRI GAGCTC 1 cut(s) 664
EcoRII CCWGG 5 cut(s) 382, 811, 952, 972, 1060
EcoT38I GRGCYC 1 cut(s) 666
FaeI CATG 8 cut(s) 97, 199, 476, 681, 850, 921, 1024, 1129
FalI AAGNNNNNCTT 2 cut(s) 573, 605
FaqI GGGAC 1 cut(s) 384
FatI CATG 8 cut(s) 93, 195, 472, 677, 846, 917, 1020, 1125
FbaI TGATCA 1 cut(s) 999
Fnu4HI GCNGC 2 cut(s) 509, 654
FokI GGATG 4 cut(s) 507, 786, 899, 1081
FriOI GRGCYC 1 cut(s) 666
Fsp4HI GCNGC 2 cut(s) 509, 654
FspBI CTAG 1 cut(s) 1143
GluI GCNGC 2 cut(s) 509, 654
HaeIII GGCC 2 cut(s) 120, 725
HapII CCGG 3 cut(s) 525, 781, 1160
HgaI GACGC 1 cut(s) 582
Hin1II CATG 8 cut(s) 97, 199, 476, 681, 850, 921, 1024, 1129
HinfI GANTC 6 cut(s) 59, 191, 236, 286, 611, 1045
HpaII CCGG 3 cut(s) 525, 781, 1160
HphI GGTGA 5 cut(s) 59, 121, 827, 1021, 1130
Hpy166II GTNNAC 3 cut(s) 730, 818, 1197
Hpy188I TCNGA 2 cut(s) 259, 1173
Hpy188III TCNNGA 3 cut(s) 347, 847, 902
Hpy8I GTNNAC 3 cut(s) 730, 818, 1197
Hpy99I CGWCG 1 cut(s) 294
HpyAV CCTTC 2 cut(s) 756, 1109
HpyCH4III ACNGT 2 cut(s) 109, 401
HpyCH4IV ACGT 1 cut(s) 994
HpyCH4V TGCA 7 cut(s) 199, 206, 307, 443, 730, 923, 1072
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 505, 517, 722, 920
HpyF3I CTNAG 7 cut(s) 256, 409, 632, 644, 658, 948, 1049
HpySE526I ACGT 1 cut(s) 994
Hsp92II CATG 8 cut(s) 97, 199, 476, 681, 850, 921, 1024, 1129
Ksp22I TGATCA 1 cut(s) 999
Kzo9I GATC 6 cut(s) 672, 685, 751, 999, 1168, 1173
LmnI GCTCC 2 cut(s) 669, 1083
Lsp1109I GCAGC 1 cut(s) 640
LweI GCATC 3 cut(s) 440, 529, 1103
MabI ACCWGGT 1 cut(s) 811
MaeI CTAG 1 cut(s) 1143
MaeII ACGT 1 cut(s) 994
MaeIII GTNAC 1 cut(s) 395
MalI GATC 6 cut(s) 674, 687, 753, 1001, 1170, 1175
MbiI CCGCTC 1 cut(s) 508
MboI GATC 6 cut(s) 672, 685, 751, 999, 1168, 1173
MboII GAAGA 4 cut(s) 434, 542, 593, 1133
MflI RGATCY 1 cut(s) 751
MhlI GDGCHC 4 cut(s) 406, 607, 666, 732
MluCI AATT 7 cut(s) 21, 207, 296, 570, 708, 897, 1088
MlyI GAGTC 2 cut(s) 200, 1039
MroXI GAANNNNTTC 1 cut(s) 251
MseI TTAA 7 cut(s) 99, 155, 330, 513, 620, 756, 1201
MslI CAYNNNNRTG 1 cut(s) 1007
MspCI CTTAAG 1 cut(s) 755
MspI CCGG 3 cut(s) 525, 781, 1160
MspR9I CCNGG 6 cut(s) 384, 525, 813, 954, 974, 1062
Mva1269I GAATGC 1 cut(s) 443
MvaI CCWGG 5 cut(s) 384, 813, 954, 974, 1062
MvnI CGCG 1 cut(s) 348
MwoI GCNNNNNNNGC 5 cut(s) 71, 505, 517, 722, 920
NciI CCSGG 1 cut(s) 525
NdeII GATC 6 cut(s) 672, 685, 751, 999, 1168, 1173
NlaIII CATG 8 cut(s) 97, 199, 476, 681, 850, 921, 1024, 1129
NlaIV GGNNCC 2 cut(s) 136, 1079
NmuCI GTSAC 1 cut(s) 395
NruI TCGCGA 1 cut(s) 348
NspI RCATGY 3 cut(s) 476, 921, 1129
OliI CACNNNNGTG 1 cut(s) 1007
PagI TCATGA 1 cut(s) 846
PciI ACATGT 1 cut(s) 917
PctI GAATGC 1 cut(s) 443
PdmI GAANNNNTTC 1 cut(s) 251
PfeI GAWTC 4 cut(s) 59, 236, 286, 611
PfoI TCCNGGA 2 cut(s) 382, 523
PkrI GCNGC 2 cut(s) 510, 655
PleI GAGTC 2 cut(s) 199, 1039
PpsI GAGTC 2 cut(s) 199, 1039
PscI ACATGT 1 cut(s) 917
Psp124BI GAGCTC 1 cut(s) 666
Psp6I CCWGG 5 cut(s) 382, 811, 952, 972, 1060
PspGI CCWGG 5 cut(s) 382, 811, 952, 972, 1060
PspN4I GGNNCC 2 cut(s) 136, 1079
PspPI GGNCC 1 cut(s) 1157
PsrI GAACNNNNNNTAC 2 cut(s) 861, 893
PsuI RGATCY 1 cut(s) 751
RruI TCGCGA 1 cut(s) 348
RsaI GTAC 1 cut(s) 870
RsaNI GTAC 1 cut(s) 869
RseI CAYNNNNRTG 1 cut(s) 1007
SacI GAGCTC 1 cut(s) 666
SaqAI TTAA 7 cut(s) 99, 155, 330, 513, 620, 756, 1201
SatI GCNGC 2 cut(s) 509, 654
Sau3AI GATC 6 cut(s) 672, 685, 751, 999, 1168, 1173
Sau96I GGNCC 1 cut(s) 1157
SchI GAGTC 2 cut(s) 200, 1039
ScrFI CCNGG 6 cut(s) 384, 525, 813, 954, 974, 1062
SduI GDGCHC 4 cut(s) 406, 607, 666, 732
SexAI ACCWGGT 1 cut(s) 811
SfaNI GCATC 3 cut(s) 440, 529, 1103
SgrAI CRCCGGYG 1 cut(s) 780
SinI GGWCC 1 cut(s) 1157
SmiMI CAYNNNNRTG 1 cut(s) 1007
SmlI CTYRAG 4 cut(s) 374, 466, 479, 755
SmoI CTYRAG 4 cut(s) 374, 466, 479, 755
Sse9I AATT 7 cut(s) 21, 207, 296, 570, 708, 897, 1088
SsiI CCGC 2 cut(s) 451, 508
SspMI CTAG 1 cut(s) 1143
SstI GAGCTC 1 cut(s) 666
StyD4I CCNGG 6 cut(s) 382, 523, 811, 952, 972, 1060
TaaI ACNGT 2 cut(s) 109, 401
TaiI ACGT 1 cut(s) 997
TaqI TCGA 3 cut(s) 292, 553, 1176
TasI AATT 7 cut(s) 21, 207, 296, 570, 708, 897, 1088
TatI WGTACW 1 cut(s) 868
TauI GCSGC 1 cut(s) 511
TfiI GAWTC 4 cut(s) 59, 236, 286, 611
Tru1I TTAA 7 cut(s) 99, 155, 330, 513, 620, 756, 1201
Tru9I TTAA 7 cut(s) 99, 155, 330, 513, 620, 756, 1201
TscAI CASTG 3 cut(s) 406, 612, 1011
TseFI GTSAC 1 cut(s) 395
TseI GCWGC 1 cut(s) 653
Tsp45I GTSAC 1 cut(s) 395
TspDTI ATGAA 5 cut(s) 17, 507, 788, 820, 863
TspGWI ACGGA 2 cut(s) 229, 1182
TspRI CASTG 3 cut(s) 406, 612, 1011
Vha464I CTTAAG 1 cut(s) 755
VneI GTGCAC 1 cut(s) 728
VpaK11BI GGWCC 1 cut(s) 1157
XceI RCATGY 3 cut(s) 476, 921, 1129
XcmI CCANNNNNNNNNTGG 1 cut(s) 128
XmnI GAANNNNTTC 1 cut(s) 251
XspI CTAG 1 cut(s) 1143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.