MD05G1364000.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0016277
Physical Location & Seq
Forward (+)
47901328 .. 47906323
4996 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1364000.v1.1.491

Sequence Viewer

Length: 819 bp
ATGGTCTCAGGTTATCTTAATGCCAACAGAGTAAGCGAAGCCATTGAACTCTCCAATTCAATGCCTCCTAGGAACACCATGTCTTGGACAACTATGGTTACAGGTCTAGCACAAAACAATGTGGCTAGACTTGCAAGGGAGTACTTTGATCGCATGCCAAATAAAGATGTAGTTGCTTGGAATGCTATGATCACATATGTCGACGAGGGACTCGTGGCAGAAGCTAGTGAGCTTTTCTGTTTGATGCGGGAAAGAAATATCGTCACTTGGAATGTGATGATTGGTGGTTATGCCAAAAGCGGAGCCAAAGAGGAAGCCTTAAAGCACCTAATTTTCATGCTTCAGTTTTGCTTTAAGCCTAATGGAACAATCATGACTAGCGTATTGAGCTCAAGTGAGGGGATGATGGAGCTCATGCAAGCTCATCTACTGGTCATATGTCATGGTAACACATTAGTTGCGAATGTTCTTATTGATATGTATGCCAGAAATGGAGATGTTACCTCTGTTAGGCATGTGTTTGAGCATCTTGGGGATAATAAGGATGCGGTATCATGGGCTGCAATGATATTGGCATTCTCAAACCATGGATATGGTCACCATGCATTGCAGGTGTTTGTACAGATGCTGAGATCCGGAGCCAAGCCAGATGGTATTACTTTTGTGGAGGTGGTATGCAGAATGCCAGCTTGCAAGCAGGATGCTGCTGTTCTAGGGGCATTGCTCGGTGCACATAAGTTGCGTGGAGACGTTACAATGGCAAATTGCATAGGAAAGCGGCTGGTGGAGTTAGAGCCAGCCAGCTCTGGGGCGTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR

Protein Analysis

273

Amino Acids

29.83

Weight (kDa)

6.5

Isoelectric Point (pI)

36.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 182 - 221 2.2e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016277)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00100
malus_domestica MD05G1364000.v1.1
prunus_persica Prupe.4G001100_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0000131
rosa_laevigata RLG00000030844
rosa_multiflora Rmu_co8477805.1_g000001 Rmu_sc0003125.1_g000003 Rmu_ssc0000243.1_g000018
rosa_roxburghii Rroxscaffold_1G00075970
rosa_rugosa Rorug04G0381800
rosa_samantha Rh5AG000900 Rh5BG002100 Rh5CG001000 Rh5DG000900
rosa_wichuraiana Rw5G000120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 601
Acc36I ACCTGC 1 cut(s) 601
AccB7I CCANNNNNTGG 1 cut(s) 84
AccI GTMKAC 1 cut(s) 201
AccIII TCCGGA 1 cut(s) 635
AciI CCGC 4 cut(s) 247, 300, 548, 778
AclWI GGATC 1 cut(s) 627
AcuI CTGAAG 1 cut(s) 326
AfaI GTAC 2 cut(s) 143, 621
AfiI CCNNNNNNNGG 3 cut(s) 84, 510, 807
AgsI TTSAA 2 cut(s) 47, 60
AluBI AGCT 7 cut(s) 224, 232, 390, 412, 422, 689, 804
AluI AGCT 7 cut(s) 224, 232, 390, 412, 422, 689, 804
Alw21I GWGCWC 3 cut(s) 392, 414, 733
Alw26I GTCTC 2 cut(s) 10, 741
Alw44I GTGCAC 1 cut(s) 729
AlwI GGATC 1 cut(s) 627
AlwNI CAGNNNCTG 1 cut(s) 628
Aor13HI TCCGGA 1 cut(s) 635
ApaLI GTGCAC 1 cut(s) 729
ApeKI GCWGC 2 cut(s) 560, 704
AspA2I CCTAGG 1 cut(s) 68
AsuHPI GGTGA 1 cut(s) 590
AvrII CCTAGG 1 cut(s) 68
BaeGI GKGCMC 1 cut(s) 733
BanII GRGCYC 2 cut(s) 392, 414
BauI CACGAG 1 cut(s) 212
Bbv12I GWGCWC 3 cut(s) 392, 414, 733
BbvI GCAGC 2 cut(s) 547, 691
BccI CCATC 2 cut(s) 400, 644
BclI TGATCA 1 cut(s) 189
BcoDI GTCTC 2 cut(s) 10, 741
BfaI CTAG 6 cut(s) 69, 107, 126, 225, 378, 713
BfuAI ACCTGC 1 cut(s) 601
BisI GCNGC 3 cut(s) 561, 705, 779
BlnI CCTAGG 1 cut(s) 68
BlsI GCNGC 3 cut(s) 562, 706, 780
BmcAI AGTACT 1 cut(s) 143
BmiI GGNNCC 2 cut(s) 304, 640
BmsI GCATC 5 cut(s) 234, 535, 535, 615, 691
BpuEI CTTGAG 1 cut(s) 376
BsaI GGTCTC 1 cut(s) 10
BsaJI CCNNGG 2 cut(s) 68, 586
BsaWI WCCGGW 1 cut(s) 635
Bsc4I CCNNNNNNNGG 3 cut(s) 84, 510, 807
Bse1I ACTGG 1 cut(s) 435
Bse3DI GCAATG 3 cut(s) 570, 605, 719
BseAI TCCGGA 1 cut(s) 635
BseDI CCNNGG 2 cut(s) 68, 586
BseGI GGATG 3 cut(s) 408, 550, 706
BseLI CCNNNNNNNGG 3 cut(s) 84, 510, 807
BseMI GCAATG 3 cut(s) 570, 605, 719
BseMII CTCAG 2 cut(s) 21, 620
BseNI ACTGG 1 cut(s) 435
BseSI GKGCMC 1 cut(s) 733
BseXI GCAGC 2 cut(s) 547, 691
BsiHKAI GWGCWC 3 cut(s) 392, 414, 733
BsiSI CCGG 1 cut(s) 636
BslFI GGGAC 1 cut(s) 222
BslI CCNNNNNNNGG 3 cut(s) 84, 510, 807
BsmAI GTCTC 2 cut(s) 10, 741
BsmBI CGTCTC 1 cut(s) 741
BsmFI GGGAC 1 cut(s) 222
BsmI GAATGC 3 cut(s) 187, 575, 687
Bso31I GGTCTC 1 cut(s) 10
Bsp1286I GDGCHC 3 cut(s) 392, 414, 733
Bsp13I TCCGGA 1 cut(s) 635
Bsp1407I TGTACA 1 cut(s) 619
Bsp143I GATC 3 cut(s) 148, 189, 632
Bsp19I CCATGG 1 cut(s) 586
BspACI CCGC 4 cut(s) 247, 300, 548, 778
BspCNI CTCAG 2 cut(s) 20, 621
BspEI TCCGGA 1 cut(s) 635
BspHI TCATGA 1 cut(s) 372
BspLI GGNNCC 2 cut(s) 304, 640
BspMI ACCTGC 1 cut(s) 601
BspPI GGATC 1 cut(s) 627
BspTNI GGTCTC 1 cut(s) 10
BsrDI GCAATG 3 cut(s) 570, 605, 719
BsrGI TGTACA 1 cut(s) 619
BsrI ACTGG 1 cut(s) 435
BssECI CCNNGG 2 cut(s) 68, 586
BssMI GATC 3 cut(s) 148, 189, 632
BssSI CACGAG 1 cut(s) 212
BssT1I CCWWGG 2 cut(s) 68, 586
Bst2BI CACGAG 1 cut(s) 212
BstAUI TGTACA 1 cut(s) 619
BstC8I GCNNGC 7 cut(s) 155, 420, 687, 691, 695, 798, 802
BstDEI CTNAG 2 cut(s) 7, 629
BstDSI CCRYGG 1 cut(s) 586
BstEII GGTNACC 1 cut(s) 596
BstENI CCTNNNNNAGG 1 cut(s) 508
BstF5I GGATG 3 cut(s) 408, 550, 706
BstKTI GATC 3 cut(s) 151, 192, 635
BstMAI GTCTC 2 cut(s) 10, 741
BstMBI GATC 3 cut(s) 148, 189, 632
BstMWI GCNNNNNNNGC 3 cut(s) 131, 182, 387
BstNSI RCATGY 2 cut(s) 157, 518
BstPI GGTNACC 1 cut(s) 596
BstSLI GKGCMC 1 cut(s) 733
BstV1I GCAGC 2 cut(s) 547, 691
BstX2I RGATCY 1 cut(s) 632
BstXI CCANNNNNNTGG 1 cut(s) 593
BstYI RGATCY 1 cut(s) 632
BtgI CCRYGG 1 cut(s) 586
BtsCI GGATG 3 cut(s) 408, 550, 706
BveI ACCTGC 1 cut(s) 601
Cac8I GCNNGC 7 cut(s) 155, 420, 687, 691, 695, 798, 802
CaiI CAGNNNCTG 1 cut(s) 628
CciI TCATGA 1 cut(s) 372
Csp6I GTAC 2 cut(s) 142, 620
CviQI GTAC 2 cut(s) 142, 620
DdeI CTNAG 2 cut(s) 7, 629
DpnI GATC 3 cut(s) 150, 191, 634
DpnII GATC 3 cut(s) 148, 189, 632
Ecl136II GAGCTC 2 cut(s) 390, 412
Eco130I CCWWGG 2 cut(s) 68, 586
Eco24I GRGCYC 2 cut(s) 392, 414
Eco31I GGTCTC 1 cut(s) 10
Eco53kI GAGCTC 2 cut(s) 390, 412
Eco57I CTGAAG 1 cut(s) 326
Eco91I GGTNACC 1 cut(s) 596
EcoICRI GAGCTC 2 cut(s) 390, 412
EcoNI CCTNNNNNAGG 1 cut(s) 508
EcoO65I GGTNACC 1 cut(s) 596
EcoT14I CCWWGG 2 cut(s) 68, 586
EcoT22I ATGCAT 1 cut(s) 607
EcoT38I GRGCYC 2 cut(s) 392, 414
ErhI CCWWGG 2 cut(s) 68, 586
Esp3I CGTCTC 1 cut(s) 741
FaqI GGGAC 1 cut(s) 222
FauI CCCGC 1 cut(s) 240
FauNDI CATATG 2 cut(s) 196, 437
FbaI TGATCA 1 cut(s) 189
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 3 cut(s) 561, 705, 779
FokI GGATG 3 cut(s) 415, 557, 713
FriOI GRGCYC 2 cut(s) 392, 414
Fsp4HI GCNGC 3 cut(s) 561, 705, 779
FspBI CTAG 6 cut(s) 69, 107, 126, 225, 378, 713
GluI GCNGC 3 cut(s) 561, 705, 779
HapII CCGG 1 cut(s) 636
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 1 cut(s) 210
HpaII CCGG 1 cut(s) 636
HphI GGTGA 1 cut(s) 590
Hpy166II GTNNAC 2 cut(s) 202, 731
Hpy188III TCNNGA 2 cut(s) 373, 636
Hpy8I GTNNAC 2 cut(s) 202, 731
Hpy99I CGWCG 1 cut(s) 206
HpyCH4IV ACGT 1 cut(s) 750
HpyCH4V TGCA 9 cut(s) 134, 418, 563, 605, 610, 678, 693, 731, 768
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 182, 387
HpyF3I CTNAG 2 cut(s) 7, 629
HpySE526I ACGT 1 cut(s) 750
Kpn2I TCCGGA 1 cut(s) 635
Ksp22I TGATCA 1 cut(s) 189
Kzo9I GATC 3 cut(s) 148, 189, 632
LmnI GCTCC 3 cut(s) 302, 409, 638
Lsp1109I GCAGC 2 cut(s) 547, 691
LweI GCATC 5 cut(s) 234, 535, 535, 615, 691
MaeI CTAG 6 cut(s) 69, 107, 126, 225, 378, 713
MaeII ACGT 1 cut(s) 750
MaeIII GTNAC 6 cut(s) 97, 262, 446, 499, 596, 751
MalI GATC 3 cut(s) 150, 191, 634
MboI GATC 3 cut(s) 148, 189, 632
MflI RGATCY 1 cut(s) 632
MhlI GDGCHC 3 cut(s) 392, 414, 733
MluCI AATT 3 cut(s) 55, 330, 763
MlyI GAGTC 1 cut(s) 204
MnlI CCTC 6 cut(s) 75, 199, 304, 391, 514, 661
Mph1103I ATGCAT 1 cut(s) 607
MroI TCCGGA 1 cut(s) 635
MseI TTAA 3 cut(s) 18, 320, 354
MslI CAYNNNNRTG 1 cut(s) 591
MspI CCGG 1 cut(s) 636
Mva1269I GAATGC 3 cut(s) 187, 575, 687
MwoI GCNNNNNNNGC 3 cut(s) 131, 182, 387
NcoI CCATGG 1 cut(s) 586
NdeI CATATG 2 cut(s) 196, 437
NdeII GATC 3 cut(s) 148, 189, 632
NlaIV GGNNCC 2 cut(s) 304, 640
NmuCI GTSAC 2 cut(s) 262, 596
NsiI ATGCAT 1 cut(s) 607
NspI RCATGY 2 cut(s) 157, 518
PaeI GCATGC 1 cut(s) 157
PagI TCATGA 1 cut(s) 372
PaqCI CACCTGC 1 cut(s) 601
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PctI GAATGC 3 cut(s) 187, 575, 687
PflMI CCANNNNNTGG 1 cut(s) 84
PkrI GCNGC 3 cut(s) 562, 706, 780
PleI GAGTC 1 cut(s) 204
PpsI GAGTC 1 cut(s) 204
Psp124BI GAGCTC 2 cut(s) 392, 414
PspEI GGTNACC 1 cut(s) 596
PspN4I GGNNCC 2 cut(s) 304, 640
PstNI CAGNNNCTG 1 cut(s) 628
PsuI RGATCY 1 cut(s) 632
RsaI GTAC 2 cut(s) 143, 621
RsaNI GTAC 2 cut(s) 142, 620
RseI CAYNNNNRTG 1 cut(s) 591
SacI GAGCTC 2 cut(s) 392, 414
SalI GTCGAC 1 cut(s) 200
SaqAI TTAA 3 cut(s) 18, 320, 354
SatI GCNGC 3 cut(s) 561, 705, 779
Sau3AI GATC 3 cut(s) 148, 189, 632
ScaI AGTACT 1 cut(s) 143
SchI GAGTC 1 cut(s) 204
SduI GDGCHC 3 cut(s) 392, 414, 733
SfaNI GCATC 5 cut(s) 234, 535, 535, 615, 691
SmiMI CAYNNNNRTG 1 cut(s) 591
SmlI CTYRAG 1 cut(s) 391
SmoI CTYRAG 1 cut(s) 391
SphI GCATGC 1 cut(s) 157
Sse9I AATT 3 cut(s) 55, 330, 763
SsiI CCGC 4 cut(s) 247, 300, 548, 778
SspMI CTAG 6 cut(s) 69, 107, 126, 225, 378, 713
SstI GAGCTC 2 cut(s) 392, 414
StyI CCWWGG 2 cut(s) 68, 586
TaiI ACGT 1 cut(s) 753
TaqI TCGA 1 cut(s) 201
TasI AATT 3 cut(s) 55, 330, 763
TatI WGTACW 2 cut(s) 141, 619
TauI GCSGC 1 cut(s) 781
Tru1I TTAA 3 cut(s) 18, 320, 354
Tru9I TTAA 3 cut(s) 18, 320, 354
TseFI GTSAC 2 cut(s) 262, 596
TseI GCWGC 2 cut(s) 560, 704
Tsp45I GTSAC 2 cut(s) 262, 596
TspDTI ATGAA 1 cut(s) 325
Van91I CCANNNNNTGG 1 cut(s) 84
VneI GTGCAC 1 cut(s) 729
XagI CCTNNNNNAGG 1 cut(s) 508
XceI RCATGY 2 cut(s) 157, 518
XmaJI CCTAGG 1 cut(s) 68
XmiI GTMKAC 1 cut(s) 201
XspI CTAG 6 cut(s) 69, 107, 126, 225, 378, 713
ZrmI AGTACT 1 cut(s) 143
Zsp2I ATGCAT 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.