Rw5G000120

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0016277
Physical Location & Seq
Reverse (-)
168638 .. 170770
2133 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G000120.1

Sequence Viewer

Length: 2115 bp
ATGAATAAAGTGGTAGCGGTTTATTGCTATATCACCCGGGCCAACCGTAAACCATTTCTGTGTTCACTTGTGACGGTATCAGTATGGATGGGCCCGCCATGTAGTAATCACATCCTCATGTCATCCTTTGCTTTCCGTCCCCGTCTTTGGAAGCTTCCCGCCATAGTTCAAACCGTAAGACAGTTGAATGCTTCTGCTCAGGTGGAGCCAAACTTGAATTCGATTGAAGCCGGTCGTCGTCATCGTCAAAAGCCAAACCTGAGTCTTTGGTGGTATAAAAGTGATACATATAGCTGTAACTGTGAGATTATAAATCTCGGGCGCTGTGGCAAAGTGAAAGATGCAAGGAAGCTGTTCGACGATATGCCTCAACGAGATGCTGTCTCTTATGCGTCCATGATCAATGTTTACTTGAAGAACGATGATCTTCCCAATGCACAGAGGCTCTTTCAGGCCATGCCCGCCAGTAATATTGTTGCTGAATCTGCAATGGTTAGTGGCTATGTCAAGGGAGGCCGTATAGCTGAGGCTCGTCAAGTTTTTGATCGCATGGAGAACAGAAATGTGTATTCTTGGACCAGTTTGATATCCGGGTATCTCAGTTGCGGACAAGTTGACGAGGCCCGTCGGCTTTTTGGTCGTATGCCTGTCAAAAATGTGGTGTCCTGGACTACTATGATCCTGGGTTACGCTCGCAACAACATGATTGATGAAGCACGCCGTGTGTTTAACCTTATGCCTGAAAGGAACACAGTCTCATGGACAGTTATGATCAAGGCTCTTATTGAGAATGATCGATTTGATGACGCACTTAGCCTCTTCAGTGATATGCCACAACGGAACCTATATTCTTGGAACATCATAATCTTGGGTCTTCTTAATGCAAATAGAGTAAGTGAAGCCATCAAACTCTTCAGTTCAATGCCTCAGAGGAATGCAATTTCTTGGACTACTATGGTTACGGGGTTGGCACATAACAAGATGACTAAACTTGCAAGAGAGTACTTTGATCGAATGCCTAACAAAGATGTAGCTGCCTGGAATGCTATGATTGCATCATATGTTGATGAAGGCCTCATGGCAGAGGCCGGTGAGCTATTCAGTTTCATGCACGAAAGGAATGTTGTTACTTGGAATCTGATGATTGATGGTTATACTAAAAGTGGGCCAAAAGAACAAGCCTTAAAGCACCTAATTCTGATGCTTCGGTTTTGCTTTAGACCTAATAGGACTACCATCGCTAGCATATTGAGCTCATGCGAGGACATGATGGAGCTAATGCAAGCTCATGCACTGGTCCAATCACATGGGTTTGAGCATGACACAGTCGTTGTGAATACACTAGTCACTATGTATTCCAGAAATGGGGATGTTACTTCTGCTAGGTTTGTTTTTGATCATCTTGGGGCTAAGGATATGGCATCATGGAATGCAATGATATTAGCATATTCAAATCATGGATATGGTCACCATGCATTGCAGGTCTTCGCACACATGTTAAGATCAGGAGCCAAGCCAGATGAAATTACTTTTGTTGGAGTCTTATCAGCTTGTAGTCATGCTGGTCTTGTCAACAAAGGTCAAAGGCTTTTCAATTCAATGAACCATGTTTATGATATAGAACCAAAGGCCCAGCACTATTCCTGCCTTGTGGACATCTTAGGCCGAGCAGGACTAGTGGATGAGGCTACGAAGGTAGTACGCATGATGCCTGCTTCTGAGAAGGATGCTGCTGTTCTAGGAGCATTGCTTGGTGCTTATAGGTTCCATGGAAATATTACAATGGCAAGTCTTATTGGAGAGGCACTTCAAGAGCTAGAGCCAGGTAGCTCTGGGGGGTATGTACTCTTAGCTATAATCTATGCTGCACGTGGAAAATGGGATGAATTTGCTCGAGTAAGAAAAAATATGAAGGAGAGGAATGTGAATAAGGTTCCTGGTTTTAGTCAAATACAAGTGAAGGGCAGGAGTCATGTATTTTTTGTGGGGGACAAATCTCATCCTCAATCTGCTGAGATTTGGGGACTGCTTCAAGAGAAGCTCCTACCACACATGTGGGAGATGGGATACTCAAAACGGAATGCATCTCAGATTGATCACCAGAAGAGGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR

Protein Analysis

704

Amino Acids

79.62

Weight (kDa)

9.3

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 190 - 219 4.7e-07 PPR repeat
PPR_2 PF13041 218 - 250 6.6e-07 PPR repeat family
PPR PF01535 221 - 250 3.2e-07 PPR repeat
PPR_2 PF13041 249 - 282 1.3e-06 PPR repeat family
PPR_2 PF13041 373 - 420 9.5e-06 PPR repeat family
PPR_2 PF13041 472 - 519 1.3e-09 PPR repeat family
E_motif PF20431 592 - 652 9e-15 E motif
Eplus_motif PF20430 658 - 678 4.9e-06 E+ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016277)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00100
malus_domestica MD05G1364000.v1.1
prunus_persica Prupe.4G001100_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0000131
rosa_laevigata RLG00000030844
rosa_multiflora Rmu_co8477805.1_g000001 Rmu_sc0003125.1_g000003 Rmu_ssc0000243.1_g000018
rosa_roxburghii Rroxscaffold_1G00075970
rosa_rugosa Rorug04G0381800
rosa_samantha Rh5AG000900 Rh5BG002100 Rh5CG001000 Rh5DG000900
rosa_wichuraiana Rw5G000120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 311
Acc36I ACCTGC 1 cut(s) 1471
AciI CCGC 5 cut(s) 17, 95, 159, 462, 606
AclWI GGATC 1 cut(s) 673
AcsI RAATTY 2 cut(s) 217, 1886
AcuI CTGAAG 2 cut(s) 805, 898
AcvI CACGTG 1 cut(s) 1871
AdeI CACNNNGTG 1 cut(s) 722
AfaI GTAC 3 cut(s) 1004, 1701, 1845
AfiI CCNNNNNNNGG 2 cut(s) 147, 1365
AflIII ACRYGT 2 cut(s) 1494, 2052
AhlI ACTAGT 2 cut(s) 1342, 1675
AjnI CCWGG 5 cut(s) 665, 681, 1037, 1822, 1936
AjuI GAANNNNNNNTTGG 2 cut(s) 202, 234
AleI CACNNNNGTG 1 cut(s) 2053
Alw21I GWGCWC 1 cut(s) 1256
Alw26I GTCTC 2 cut(s) 388, 760
AlwI GGATC 1 cut(s) 673
Ama87I CYCGRG 3 cut(s) 36, 317, 1893
ApaI GGGCCC 1 cut(s) 95
ApeKI GCWGC 3 cut(s) 1034, 1730, 1865
ApoI RAATTY 2 cut(s) 217, 1886
Asp700I GAANNNNTTC 1 cut(s) 353
AspLEI GCGC 1 cut(s) 324
AspS9I GGNCC 8 cut(s) 39, 91, 92, 576, 622, 1166, 1297, 1630
AsuC2I CCSGG 3 cut(s) 37, 38, 592
AsuHPI GGTGA 4 cut(s) 25, 1103, 1460, 2090
AsuNHI GCTAGC 1 cut(s) 1241
AvaI CYCGRG 3 cut(s) 36, 317, 1893
AvaII GGWCC 2 cut(s) 576, 1297
BaeGI GKGCMC 1 cut(s) 95
BanII GRGCYC 2 cut(s) 95, 1256
BbrPI CACGTG 1 cut(s) 1871
BbsI GAAGAC 2 cut(s) 866, 1477
Bbv12I GWGCWC 1 cut(s) 1256
BbvCI CCTCAGC 1 cut(s) 525
BbvI GCAGC 3 cut(s) 1021, 1717, 1852
BccI CCATC 6 cut(s) 82, 911, 1142, 1244, 1264, 2056
BceAI ACGGC 2 cut(s) 501, 705
BciT130I CCWGG 5 cut(s) 667, 683, 1039, 1824, 1938
BciVI GTATCC 1 cut(s) 2060
BclI TGATCA 4 cut(s) 399, 771, 1396, 2095
BcnI CCSGG 3 cut(s) 37, 38, 592
BcoDI GTCTC 2 cut(s) 388, 760
BcuI ACTAGT 2 cut(s) 1342, 1675
BfaI CTAG 6 cut(s) 1242, 1343, 1383, 1676, 1739, 1817
BfoI RGCGCY 1 cut(s) 325
BfuAI ACCTGC 1 cut(s) 1471
BfuI GTATCC 1 cut(s) 2060
BisI GCNGC 3 cut(s) 1035, 1731, 1866
BlsI GCNGC 3 cut(s) 1036, 1732, 1867
BmcAI AGTACT 1 cut(s) 1004
Bme1390I CCNGG 8 cut(s) 37, 38, 592, 667, 683, 1039, 1824, 1938
Bme18I GGWCC 2 cut(s) 576, 1297
BmeT110I CYCGRG 3 cut(s) 36, 317, 1893
BmgT120I GGNCC 8 cut(s) 39, 91, 92, 576, 622, 1166, 1297, 1630
BmiI GGNNCC 6 cut(s) 93, 207, 842, 1510, 1766, 1935
BmrFI CCNGG 8 cut(s) 37, 38, 592, 667, 683, 1039, 1824, 1938
BmsI GCATC 8 cut(s) 331, 367, 1064, 1191, 1430, 1698, 1717, 2093
BmtI GCTAGC 1 cut(s) 1245
BpiI GAAGAC 2 cut(s) 866, 1477
Bpu10I CCTNAGC 3 cut(s) 198, 525, 1410
BpuMI CCSGG 3 cut(s) 37, 38, 592
Bsa29I ATCGAT 1 cut(s) 796
BsaAI YACGTR 1 cut(s) 1871
BsaJI CCNNGG 3 cut(s) 36, 682, 1768
Bsc4I CCNNNNNNNGG 2 cut(s) 147, 1365
Bse118I RCCGGY 2 cut(s) 230, 1088
Bse1I ACTGG 3 cut(s) 465, 579, 1299
Bse3DI GCAATG 4 cut(s) 495, 1440, 1475, 1745
BseBI CCWGG 5 cut(s) 667, 683, 1039, 1824, 1938
BseCI ATCGAT 1 cut(s) 796
BseDI CCNNGG 3 cut(s) 36, 682, 1768
BseGI GGATG 8 cut(s) 93, 111, 122, 1375, 1687, 1732, 1888, 1999
BseLI CCNNNNNNNGG 2 cut(s) 147, 1365
BseMI GCAATG 4 cut(s) 495, 1440, 1475, 1745
BseMII CTCAG 8 cut(s) 212, 251, 516, 613, 941, 1710, 2004, 2102
BseNI ACTGG 3 cut(s) 465, 579, 1299
BseSI GKGCMC 1 cut(s) 95
BseXI GCAGC 3 cut(s) 1021, 1717, 1852
BseYI CCCAGC 1 cut(s) 1632
BsgI GTGCAG 1 cut(s) 1851
Bsh1285I CGRYCG 1 cut(s) 235
BshVI ATCGAT 1 cut(s) 796
BsiEI CGRYCG 1 cut(s) 235
BsiHKAI GWGCWC 1 cut(s) 1256
BsiHKCI CYCGRG 3 cut(s) 36, 317, 1893
BsiSI CCGG 4 cut(s) 37, 231, 591, 1089
BslFI GGGAC 3 cut(s) 123, 2003, 2037
BslI CCNNNNNNNGG 2 cut(s) 147, 1365
BsmAI GTCTC 2 cut(s) 388, 760
BsmFI GGGAC 3 cut(s) 123, 2003, 2037
BsmI GAATGC 6 cut(s) 193, 940, 1020, 1048, 1435, 2086
BsoBI CYCGRG 3 cut(s) 36, 317, 1893
Bsp120I GGGCCC 1 cut(s) 91
Bsp1286I GDGCHC 2 cut(s) 95, 1256
Bsp19I CCATGG 1 cut(s) 1768
BspACI CCGC 5 cut(s) 17, 95, 159, 462, 606
BspCNI CTCAG 8 cut(s) 211, 252, 517, 612, 940, 1711, 2005, 2101
BspDI ATCGAT 1 cut(s) 796
BspLI GGNNCC 6 cut(s) 93, 207, 842, 1510, 1766, 1935
BspMI ACCTGC 1 cut(s) 1471
BspOI GCTAGC 1 cut(s) 1245
BspPI GGATC 1 cut(s) 673
BsrDI GCAATG 4 cut(s) 495, 1440, 1475, 1745
BsrFI RCCGGY 2 cut(s) 230, 1088
BsrI ACTGG 3 cut(s) 465, 579, 1299
BssAI RCCGGY 2 cut(s) 230, 1088
BssECI CCNNGG 3 cut(s) 36, 682, 1768
BssT1I CCWWGG 1 cut(s) 1768
Bst2UI CCWGG 5 cut(s) 667, 683, 1039, 1824, 1938
Bst4CI ACNGT 8 cut(s) 47, 76, 175, 183, 302, 754, 766, 1327
Bst6I CTCTTC 3 cut(s) 824, 917, 2099
BstBAI YACGTR 1 cut(s) 1871
BstC8I GCNNGC 7 cut(s) 95, 462, 694, 718, 1243, 1284, 1713
BstDSI CCRYGG 1 cut(s) 1768
BstEII GGTNACC 1 cut(s) 1466
BstF5I GGATG 8 cut(s) 93, 111, 122, 1375, 1687, 1732, 1888, 1999
BstH2I RGCGCY 1 cut(s) 325
BstHHI GCGC 1 cut(s) 324
BstMAI GTCTC 2 cut(s) 388, 760
BstMCI CGRYCG 1 cut(s) 235
BstMWI GCNNNNNNNGC 5 cut(s) 461, 485, 1043, 1052, 1251
BstNI CCWGG 5 cut(s) 667, 683, 1039, 1824, 1938
BstNSI RCATGY 2 cut(s) 1498, 2056
BstPI GGTNACC 1 cut(s) 1466
BstSCI CCNGG 8 cut(s) 35, 36, 590, 665, 681, 1037, 1822, 1936
BstSLI GKGCMC 1 cut(s) 95
BstV1I GCAGC 3 cut(s) 1021, 1717, 1852
BstV2I GAAGAC 2 cut(s) 866, 1477
BstXI CCANNNNNNTGG 2 cut(s) 1307, 2055
Bsu15I ATCGAT 1 cut(s) 796
BsuI GTATCC 1 cut(s) 2060
BsuTUI ATCGAT 1 cut(s) 796
BtgI CCRYGG 1 cut(s) 1768
BtgZI GCGATG 1 cut(s) 1222
BtsCI GGATG 8 cut(s) 93, 111, 122, 1375, 1687, 1732, 1888, 1999
BtsIMutI CAGTG 2 cut(s) 829, 1292
BveI ACCTGC 1 cut(s) 1471
Cac8I GCNNGC 7 cut(s) 95, 462, 694, 718, 1243, 1284, 1713
CfoI GCGC 1 cut(s) 324
Cfr10I RCCGGY 2 cut(s) 230, 1088
Cfr13I GGNCC 8 cut(s) 39, 91, 92, 576, 622, 1166, 1297, 1630
Cfr9I CCCGGG 1 cut(s) 36
ClaI ATCGAT 1 cut(s) 796
CseI GACGC 2 cut(s) 381, 815
Csp6I GTAC 3 cut(s) 1003, 1700, 1844
CviQI GTAC 3 cut(s) 1003, 1700, 1844
DraIII CACNNNGTG 1 cut(s) 722
Eam1104I CTCTTC 3 cut(s) 824, 917, 2099
EarI CTCTTC 3 cut(s) 824, 917, 2099
Ecl136II GAGCTC 1 cut(s) 1254
Eco130I CCWWGG 1 cut(s) 1768
Eco147I AGGCCT 1 cut(s) 1074
Eco24I GRGCYC 2 cut(s) 95, 1256
Eco32I GATATC 1 cut(s) 588
Eco47I GGWCC 2 cut(s) 576, 1297
Eco53kI GAGCTC 1 cut(s) 1254
Eco57I CTGAAG 2 cut(s) 805, 898
Eco72I CACGTG 1 cut(s) 1871
Eco88I CYCGRG 3 cut(s) 36, 317, 1893
Eco91I GGTNACC 1 cut(s) 1466
EcoICRI GAGCTC 1 cut(s) 1254
EcoO65I GGTNACC 1 cut(s) 1466
EcoRI GAATTC 1 cut(s) 217
EcoRII CCWGG 5 cut(s) 665, 681, 1037, 1822, 1936
EcoRV GATATC 1 cut(s) 588
EcoT14I CCWWGG 1 cut(s) 1768
EcoT22I ATGCAT 2 cut(s) 1477, 2086
EcoT38I GRGCYC 2 cut(s) 95, 1256
ErhI CCWWGG 1 cut(s) 1768
FaqI GGGAC 3 cut(s) 123, 2003, 2037
FauI CCCGC 3 cut(s) 102, 166, 469
FauNDI CATATG 1 cut(s) 1060
FbaI TGATCA 4 cut(s) 399, 771, 1396, 2095
Fnu4HI GCNGC 3 cut(s) 1035, 1731, 1866
FokI GGATG 8 cut(s) 98, 100, 109, 1382, 1694, 1739, 1895, 1986
FriOI GRGCYC 2 cut(s) 95, 1256
Fsp4HI GCNGC 3 cut(s) 1035, 1731, 1866
FspBI CTAG 6 cut(s) 1242, 1343, 1383, 1676, 1739, 1817
GlaI GCGC 1 cut(s) 323
GluI GCNGC 3 cut(s) 1035, 1731, 1866
GsaI CCCAGC 1 cut(s) 1636
HaeII RGCGCY 1 cut(s) 325
HapII CCGG 4 cut(s) 37, 231, 591, 1089
HgaI GACGC 2 cut(s) 381, 815
HhaI GCGC 1 cut(s) 324
Hin6I GCGC 1 cut(s) 322
HinP1I GCGC 1 cut(s) 322
HincII GTYRAC 2 cut(s) 616, 1573
HindII GTYRAC 2 cut(s) 616, 1573
HindIII AAGCTT 1 cut(s) 152
HinfI GANTC 5 cut(s) 262, 482, 1135, 1539, 1969
HpaII CCGG 4 cut(s) 37, 231, 591, 1089
HphI GGTGA 4 cut(s) 25, 1103, 1460, 2090
Hpy166II GTNNAC 6 cut(s) 50, 65, 409, 616, 1573, 1654
Hpy188I TCNGA 5 cut(s) 930, 1140, 1200, 1720, 2091
Hpy188III TCNNGA 4 cut(s) 1359, 1506, 1811, 2033
Hpy8I GTNNAC 6 cut(s) 50, 65, 409, 616, 1573, 1654
Hpy99I CGWCG 3 cut(s) 240, 362, 630
HpyAV CCTTC 5 cut(s) 1064, 1687, 1717, 1906, 1954
HpyCH4III ACNGT 8 cut(s) 47, 76, 175, 183, 302, 754, 766, 1327
HpyCH4IV ACGT 1 cut(s) 1870
HpyF10VI GCNNNNNNNGC 5 cut(s) 461, 485, 1043, 1052, 1251
HpySE526I ACGT 1 cut(s) 1870
HspAI GCGC 1 cut(s) 322
Ksp22I TGATCA 4 cut(s) 399, 771, 1396, 2095
LmnI GCTCC 5 cut(s) 205, 1273, 1508, 1742, 2046
Lsp1109I GCAGC 3 cut(s) 1021, 1717, 1852
LweI GCATC 8 cut(s) 331, 367, 1064, 1191, 1430, 1698, 1717, 2093
MaeI CTAG 6 cut(s) 1242, 1343, 1383, 1676, 1739, 1817
MaeII ACGT 1 cut(s) 1870
MaeIII GTNAC 8 cut(s) 70, 296, 686, 958, 1126, 1345, 1372, 1466
MboII GAAGA 6 cut(s) 419, 427, 811, 866, 904, 1477
MhlI GDGCHC 2 cut(s) 95, 1256
MluCI AATT 7 cut(s) 217, 939, 1194, 1524, 1594, 1886, 2110
MlyI GAGTC 3 cut(s) 271, 1548, 1978
MmeI TCCRAC 1 cut(s) 1516
Mph1103I ATGCAT 2 cut(s) 1477, 2086
MroXI GAANNNNTTC 1 cut(s) 353
MseI TTAA 4 cut(s) 729, 879, 1184, 1499
MslI CAYNNNNRTG 5 cut(s) 58, 116, 1461, 1611, 2053
MspI CCGG 4 cut(s) 37, 231, 591, 1089
MspR9I CCNGG 8 cut(s) 37, 38, 592, 667, 683, 1039, 1824, 1938
Mva1269I GAATGC 6 cut(s) 193, 940, 1020, 1048, 1435, 2086
MvaI CCWGG 5 cut(s) 667, 683, 1039, 1824, 1938
MwoI GCNNNNNNNGC 5 cut(s) 461, 485, 1043, 1052, 1251
NciI CCSGG 3 cut(s) 37, 38, 592
NcoI CCATGG 1 cut(s) 1768
NdeI CATATG 1 cut(s) 1060
NheI GCTAGC 1 cut(s) 1241
NlaIV GGNNCC 6 cut(s) 93, 207, 842, 1510, 1766, 1935
NmeAIII GCCGAG 1 cut(s) 1691
NmuCI GTSAC 3 cut(s) 70, 1345, 1466
NsiI ATGCAT 2 cut(s) 1477, 2086
NspI RCATGY 2 cut(s) 1498, 2056
OliI CACNNNNGTG 1 cut(s) 2053
PaeR7I CTCGAG 1 cut(s) 1893
PceI AGGCCT 1 cut(s) 1074
PciI ACATGT 2 cut(s) 1494, 2052
PcsI WCGNNNNNNNCGW 1 cut(s) 241
PctI GAATGC 6 cut(s) 193, 940, 1020, 1048, 1435, 2086
PdmI GAANNNNTTC 1 cut(s) 353
PfeI GAWTC 2 cut(s) 482, 1135
PflFI GACNNNGTC 1 cut(s) 1325
PfoI TCCNGGA 1 cut(s) 665
PkrI GCNGC 3 cut(s) 1036, 1732, 1867
PleI GAGTC 3 cut(s) 270, 1547, 1977
PmaCI CACGTG 1 cut(s) 1871
PmlI CACGTG 1 cut(s) 1871
PpsI GAGTC 3 cut(s) 270, 1547, 1977
Ppu21I YACGTR 1 cut(s) 1871
PscI ACATGT 2 cut(s) 1494, 2052
PsiI TTATAA 1 cut(s) 311
Psp124BI GAGCTC 1 cut(s) 1256
Psp6I CCWGG 5 cut(s) 665, 681, 1037, 1822, 1936
PspCI CACGTG 1 cut(s) 1871
PspEI GGTNACC 1 cut(s) 1466
PspFI CCCAGC 1 cut(s) 1632
PspGI CCWGG 5 cut(s) 665, 681, 1037, 1822, 1936
PspN4I GGNNCC 6 cut(s) 93, 207, 842, 1510, 1766, 1935
PspOMI GGGCCC 1 cut(s) 91
PspPI GGNCC 8 cut(s) 39, 91, 92, 576, 622, 1166, 1297, 1630
PspXI VCTCGAGB 1 cut(s) 1893
PsyI GACNNNGTC 1 cut(s) 1325
RsaI GTAC 3 cut(s) 1004, 1701, 1845
RsaNI GTAC 3 cut(s) 1003, 1700, 1844
RseI CAYNNNNRTG 5 cut(s) 58, 116, 1461, 1611, 2053
SacI GAGCTC 1 cut(s) 1256
SaqAI TTAA 4 cut(s) 729, 879, 1184, 1499
SatI GCNGC 3 cut(s) 1035, 1731, 1866
Sau96I GGNCC 8 cut(s) 39, 91, 92, 576, 622, 1166, 1297, 1630
ScaI AGTACT 1 cut(s) 1004
SchI GAGTC 3 cut(s) 271, 1548, 1978
ScrFI CCNGG 8 cut(s) 37, 38, 592, 667, 683, 1039, 1824, 1938
SduI GDGCHC 2 cut(s) 95, 1256
SfaNI GCATC 8 cut(s) 331, 367, 1064, 1191, 1430, 1698, 1717, 2093
Sfr274I CTCGAG 1 cut(s) 1893
SinI GGWCC 2 cut(s) 576, 1297
SlaI CTCGAG 1 cut(s) 1893
SmaI CCCGGG 1 cut(s) 38
SmiMI CAYNNNNRTG 5 cut(s) 58, 116, 1461, 1611, 2053
SmlI CTYRAG 1 cut(s) 1893
SmoI CTYRAG 1 cut(s) 1893
SpeI ACTAGT 2 cut(s) 1342, 1675
Sse9I AATT 7 cut(s) 217, 939, 1194, 1524, 1594, 1886, 2110
SseBI AGGCCT 1 cut(s) 1074
SsiI CCGC 5 cut(s) 17, 95, 159, 462, 606
SspI AATATT 2 cut(s) 472, 1777
SspMI CTAG 6 cut(s) 1242, 1343, 1383, 1676, 1739, 1817
SstI GAGCTC 1 cut(s) 1256
StuI AGGCCT 1 cut(s) 1074
StyD4I CCNGG 8 cut(s) 35, 36, 590, 665, 681, 1037, 1822, 1936
StyI CCWWGG 1 cut(s) 1768
TaaI ACNGT 8 cut(s) 47, 76, 175, 183, 302, 754, 766, 1327
TaiI ACGT 1 cut(s) 1873
TaqI TCGA 5 cut(s) 221, 357, 796, 1012, 1894
TasI AATT 7 cut(s) 217, 939, 1194, 1524, 1594, 1886, 2110
TatI WGTACW 2 cut(s) 1002, 1843
TfiI GAWTC 2 cut(s) 482, 1135
Tru1I TTAA 4 cut(s) 729, 879, 1184, 1499
Tru9I TTAA 4 cut(s) 729, 879, 1184, 1499
TscAI CASTG 2 cut(s) 829, 1299
TseFI GTSAC 3 cut(s) 70, 1345, 1466
TseI GCWGC 3 cut(s) 1034, 1730, 1865
Tsp45I GTSAC 3 cut(s) 70, 1345, 1466
TspDTI ATGAA 8 cut(s) 17, 726, 1083, 1096, 1536, 1616, 1899, 1925
TspGWI ACGGA 3 cut(s) 125, 853, 2092
TspMI CCCGGG 1 cut(s) 36
TspRI CASTG 2 cut(s) 829, 1299
Tth111I GACNNNGTC 1 cut(s) 1325
VpaK11BI GGWCC 2 cut(s) 576, 1297
XapI RAATTY 2 cut(s) 217, 1886
XceI RCATGY 2 cut(s) 1498, 2056
XhoI CTCGAG 1 cut(s) 1893
XmaI CCCGGG 1 cut(s) 36
XmnI GAANNNNTTC 1 cut(s) 353
XspI CTAG 6 cut(s) 1242, 1343, 1383, 1676, 1739, 1817
ZrmI AGTACT 1 cut(s) 1004
Zsp2I ATGCAT 2 cut(s) 1477, 2086
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.