MD06G1027300.v1.1

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0014386
Physical Location & Seq
Forward (+)
3351729 .. 3355059
3331 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1027300.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGGAGGGGTTACAACGTTGTAGATATTGGTTTAATGGAGTTTCTCTCTTTTTCCATCCTTTGAGGTTAGTACTATTGATCCTTGTGTGTTTTGGAAACGTAGGTGCAGCGTTCTATAACCTGACCACCATCCCCTTCGACCAAGGCTATATCCCTCTCTTCGGCGACGGCAACCTTGTCCGCTCCCCCGACGGAAAAGGTGTTCGTCTCCTCCTTGATCGCTTCACAGGTTCTGGCTTCATCTCCTCCAATCTTTACAACCATGGTTTCTTCAGCGCTAACATTAAGTTGCCATCCGATTACACTGCCGGTATCTGTGTTGCTTTTTACACATCAAACGCGGACGTATTCGAAAAGAGCCACGATGAGTTGGACATAGAATTCTTAGGGAACACAGAAGGGAAGCCATGGAGATTCCAAACCAACATCTACGGAAATGGCAGCACCAACCGCGGCCGCGAAGAGCGTTACCGCCTCTGGTTCGACCCTACCAAAGACTTCCACCGCTACAGCATCCTTTGGACCCCCGAAAAAATCATATTCTACGTGGATGAAGTTCCAATTAGAGAGGTGGTGCGAAATGAAGAAATGGGTGCTGACTATCCTTCAAAGCCAATGTCGTTGTATACGACTATATGGGATGCAAGCAGTTGGGCTACGTCCGGTGGGAAATACAAGGTGAATTACAAATATGCCCCCTTCGTGGCAGAGTTCAAAGACCTAGCCCTAGAGGGCTGCCCTGCCGACCCAATCCAGCAAATCCCTCCTGCTGAAGCGTGTGCGGAACATTACACCCACCTCGCCACCCAAGACTACTCCGTCATTTCGCCTCAGCGGCGAGCTGCCATGAGCAGGTTTCGGCAGCGTTACATGTACTACTCATATTGCTATGATTCGCTGCGTTACCCAGTGCCACCACCCGAGTGCGTTACCGTTCCTGCCGAGAAGGCACGCTTCAAGGACACTGGCAGGTTGAAGTTTGGCGGCAGCCACAAACGACAGTCCAAGCGGAAGTCCCACAAACGCAACCGAGCAGTCCCTAACTCCGACAACGCTGCAGACATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

356

Amino Acids

40.73

Weight (kDa)

8.79

Isoelectric Point (pI)

45.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 54 - 230 9.9e-57 Glycosyl hydrolases family 16
XET_C PF06955 273 - 309 7.5e-14 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014386)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32170
fragaria_vesca FvH4_5g23180 FvH4_5g23180
malus_domestica MD06G1027300.v1.1 MD16G1278900.v1.1
prunus_persica Prupe.5G027800_v2.0.a1
pyrus_communis pycom06g02230 pycom16g24910
rosa_chinensis RchiOBHm_Chr7g0212101
rosa_laevigata RLG00000002935
rosa_multiflora Rmu_sc0002283.1_g000035
rosa_roxburghii Rroxscaffold_3G00247140
rosa_rugosa Rorug07G0131000
rosa_samantha Rh7AG263700 Rh7BG257600 Rh7DG270300
rosa_wichuraiana Rw7G022520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 818
Acc36I ACCTGC 2 cut(s) 843, 960
AccBSI CCGCTC 1 cut(s) 183
AccI GTMKAC 1 cut(s) 626
AccII CGCG 3 cut(s) 341, 453, 459
AclI AACGTT 1 cut(s) 16
AclWI GGATC 1 cut(s) 73
AcoI YGGCCR 1 cut(s) 454
AcsI RAATTY 1 cut(s) 380
AcuI CTGAAG 2 cut(s) 256, 792
AfaI GTAC 2 cut(s) 72, 875
AfeI AGCGCT 1 cut(s) 277
AfiI CCNNNNNNNGG 3 cut(s) 161, 703, 852
AflIII ACRYGT 1 cut(s) 870
AgsI TTSAA 4 cut(s) 609, 715, 958, 976
AleI CACNNNNGTG 1 cut(s) 922
AloI GAACNNNNNNTCC 2 cut(s) 186, 218
AluBI AGCT 1 cut(s) 842
AluI AGCT 1 cut(s) 842
Alw26I GTCTC 1 cut(s) 212
AlwI GGATC 1 cut(s) 73
AlwNI CAGNNNCTG 1 cut(s) 233
Ama87I CYCGRG 1 cut(s) 920
Aor51HI AGCGCT 1 cut(s) 277
AoxI GGCC 1 cut(s) 454
ApeKI GCWGC 8 cut(s) 107, 441, 735, 842, 862, 898, 987, 1055
ApoI RAATTY 1 cut(s) 380
ArsI GACNNNNNNTTYG 2 cut(s) 602, 634
AspLEI GCGC 1 cut(s) 278
AspS9I GGNCC 1 cut(s) 522
AsuHPI GGTGA 1 cut(s) 691
AsuII TTCGAA 1 cut(s) 351
AvaI CYCGRG 1 cut(s) 920
AvaII GGWCC 1 cut(s) 522
BbvCI CCTCAGC 1 cut(s) 831
BbvI GCAGC 8 cut(s) 119, 453, 722, 829, 874, 885, 999, 1042
BccI CCATC 3 cut(s) 63, 137, 301
BceAI ACGGC 1 cut(s) 184
BcgI CGANNNNNNTGC 3 cut(s) 287, 321, 1037
BcoDI GTCTC 1 cut(s) 212
BfaI CTAG 2 cut(s) 722, 728
BfmI CTRYAG 2 cut(s) 508, 1056
BfoI RGCGCY 1 cut(s) 279
BfuAI ACCTGC 2 cut(s) 843, 960
BglI GCCNNNNNGGC 2 cut(s) 835, 947
BmcAI AGTACT 1 cut(s) 72
Bme18I GGWCC 1 cut(s) 522
BmeT110I CYCGRG 1 cut(s) 920
BmgT120I GGNCC 1 cut(s) 522
BmiI GGNNCC 1 cut(s) 524
BmrI ACTGGG 1 cut(s) 902
BmsI GCATC 2 cut(s) 522, 631
BmuI ACTGGG 1 cut(s) 902
BplI GAGNNNNNCTC 2 cut(s) 30, 62
Bpu10I CCTNAGC 1 cut(s) 831
Bpu14I TTCGAA 1 cut(s) 351
BsaAI YACGTR 1 cut(s) 547
BsaJI CCNNGG 4 cut(s) 142, 262, 407, 451
BsaWI WCCGGW 1 cut(s) 662
Bsc4I CCNNNNNNNGG 3 cut(s) 161, 703, 852
Bse118I RCCGGY 1 cut(s) 308
Bse1I ACTGG 2 cut(s) 908, 970
BseDI CCNNGG 4 cut(s) 142, 262, 407, 451
BseGI GGATG 6 cut(s) 55, 129, 293, 513, 556, 646
BseLI CCNNNNNNNGG 3 cut(s) 161, 703, 852
BseMII CTCAG 1 cut(s) 845
BseNI ACTGG 2 cut(s) 908, 970
BseRI GAGGAG 2 cut(s) 200, 235
BseX3I CGGCCG 1 cut(s) 454
BseXI GCAGC 8 cut(s) 119, 453, 722, 829, 874, 885, 999, 1042
BsgI GTGCAG 1 cut(s) 126
Bsh1236I CGCG 3 cut(s) 341, 453, 459
Bsh1285I CGRYCG 1 cut(s) 457
BshFI GGCC 1 cut(s) 456
BsiEI CGRYCG 1 cut(s) 457
BsiHKCI CYCGRG 1 cut(s) 920
BsiSI CCGG 2 cut(s) 309, 663
BslFI GGGAC 2 cut(s) 1000, 1022
BslI CCNNNNNNNGG 3 cut(s) 161, 703, 852
BsmAI GTCTC 1 cut(s) 212
BsmBI CGTCTC 1 cut(s) 212
BsmFI GGGAC 2 cut(s) 1000, 1022
BsnI GGCC 1 cut(s) 456
BsoBI CYCGRG 1 cut(s) 920
Bsp119I TTCGAA 1 cut(s) 351
Bsp143I GATC 2 cut(s) 78, 217
Bsp19I CCATGG 2 cut(s) 262, 407
BspANI GGCC 1 cut(s) 456
BspCNI CTCAG 1 cut(s) 844
BspFNI CGCG 3 cut(s) 341, 453, 459
BspLI GGNNCC 1 cut(s) 524
BspMAI CTGCAG 1 cut(s) 1060
BspMI ACCTGC 2 cut(s) 843, 960
BspPI GGATC 1 cut(s) 73
BspQI GCTCTTC 1 cut(s) 456
BspT104I TTCGAA 1 cut(s) 351
BsrBI CCGCTC 1 cut(s) 183
BsrFI RCCGGY 1 cut(s) 308
BsrI ACTGG 2 cut(s) 908, 970
BssAI RCCGGY 1 cut(s) 308
BssECI CCNNGG 4 cut(s) 142, 262, 407, 451
BssMI GATC 2 cut(s) 78, 217
BssNAI GTATAC 1 cut(s) 627
BssT1I CCWWGG 3 cut(s) 142, 262, 407
Bst1107I GTATAC 1 cut(s) 627
Bst4CI ACNGT 2 cut(s) 934, 1002
Bst6I CTCTTC 2 cut(s) 164, 456
BstBAI YACGTR 1 cut(s) 547
BstBI TTCGAA 1 cut(s) 351
BstC8I GCNNGC 3 cut(s) 646, 840, 952
BstDEI CTNAG 2 cut(s) 385, 831
BstDSI CCRYGG 3 cut(s) 262, 407, 451
BstF5I GGATG 6 cut(s) 55, 129, 293, 513, 556, 646
BstFNI CGCG 3 cut(s) 341, 453, 459
BstH2I RGCGCY 1 cut(s) 279
BstHHI GCGC 1 cut(s) 278
BstKTI GATC 2 cut(s) 81, 220
BstMAI GTCTC 1 cut(s) 212
BstMBI GATC 2 cut(s) 78, 217
BstMCI CGRYCG 1 cut(s) 457
BstMWI GCNNNNNNNGC 3 cut(s) 450, 835, 947
BstNSI RCATGY 1 cut(s) 874
BstSFI CTRYAG 2 cut(s) 508, 1056
BstUI CGCG 3 cut(s) 341, 453, 459
BstV1I GCAGC 8 cut(s) 119, 453, 722, 829, 874, 885, 999, 1042
BstZ17I GTATAC 1 cut(s) 627
BstZI CGGCCG 1 cut(s) 454
BsuRI GGCC 1 cut(s) 456
BtgI CCRYGG 3 cut(s) 262, 407, 451
BtsCI GGATG 6 cut(s) 55, 129, 293, 513, 556, 646
BtsI GCAGTG 1 cut(s) 303
BtsIMutI CAGTG 3 cut(s) 303, 915, 963
BveI ACCTGC 2 cut(s) 843, 960
Cac8I GCNNGC 3 cut(s) 646, 840, 952
CaiI CAGNNNCTG 1 cut(s) 233
CciNI GCGGCCGC 1 cut(s) 454
CfoI GCGC 1 cut(s) 278
Cfr10I RCCGGY 1 cut(s) 308
Cfr13I GGNCC 1 cut(s) 522
Cfr42I CCGCGG 1 cut(s) 454
Csp6I GTAC 2 cut(s) 71, 874
CviAII CATG 4 cut(s) 263, 408, 847, 871
CviQI GTAC 2 cut(s) 71, 874
DdeI CTNAG 2 cut(s) 385, 831
DpnI GATC 2 cut(s) 80, 219
DpnII GATC 2 cut(s) 78, 217
DrdI GACNNNNNNGTC 1 cut(s) 818
DseDI GACNNNNNNGTC 1 cut(s) 818
EaeI YGGCCR 1 cut(s) 454
EagI CGGCCG 1 cut(s) 454
Eam1104I CTCTTC 2 cut(s) 164, 456
EarI CTCTTC 2 cut(s) 164, 456
EclXI CGGCCG 1 cut(s) 454
Eco130I CCWWGG 3 cut(s) 142, 262, 407
Eco47I GGWCC 1 cut(s) 522
Eco47III AGCGCT 1 cut(s) 277
Eco52I CGGCCG 1 cut(s) 454
Eco57I CTGAAG 2 cut(s) 256, 792
Eco88I CYCGRG 1 cut(s) 920
EcoRI GAATTC 1 cut(s) 380
EcoT14I CCWWGG 3 cut(s) 142, 262, 407
ErhI CCWWGG 3 cut(s) 142, 262, 407
Esp3I CGTCTC 1 cut(s) 212
FaeI CATG 4 cut(s) 266, 411, 850, 874
FalI AAGNNNNNCTT 2 cut(s) 938, 970
FaqI GGGAC 2 cut(s) 1000, 1022
FatI CATG 4 cut(s) 262, 407, 846, 870
FauNDI CATATG 1 cut(s) 1064
FblI GTMKAC 1 cut(s) 626
FokI GGATG 6 cut(s) 42, 116, 280, 500, 563, 653
FspBI CTAG 2 cut(s) 722, 728
GlaI GCGC 1 cut(s) 277
HaeII RGCGCY 1 cut(s) 279
HaeIII GGCC 1 cut(s) 456
HapII CCGG 2 cut(s) 309, 663
HhaI GCGC 1 cut(s) 278
Hin1II CATG 4 cut(s) 266, 411, 850, 874
Hin6I GCGC 1 cut(s) 276
HinP1I GCGC 1 cut(s) 276
HinfI GANTC 2 cut(s) 414, 893
HpaII CCGG 2 cut(s) 309, 663
HphI GGTGA 1 cut(s) 691
Hpy166II GTNNAC 1 cut(s) 627
Hpy188I TCNGA 2 cut(s) 298, 1048
Hpy8I GTNNAC 1 cut(s) 627
Hpy99I CGWCG 2 cut(s) 170, 194
HpyAV CCTTC 5 cut(s) 145, 392, 615, 709, 940
HpyCH4III ACNGT 2 cut(s) 934, 1002
HpyCH4IV ACGT 5 cut(s) 16, 99, 345, 546, 659
HpyCH4V TGCA 3 cut(s) 107, 644, 1058
HpyF10VI GCNNNNNNNGC 3 cut(s) 450, 835, 947
HpyF3I CTNAG 2 cut(s) 385, 831
HpySE526I ACGT 5 cut(s) 16, 99, 345, 546, 659
Hsp92II CATG 4 cut(s) 266, 411, 850, 874
HspAI GCGC 1 cut(s) 276
KspI CCGCGG 1 cut(s) 454
Kzo9I GATC 2 cut(s) 78, 217
LguI GCTCTTC 1 cut(s) 456
LmnI GCTCC 1 cut(s) 188
Lsp1109I GCAGC 8 cut(s) 119, 453, 722, 829, 874, 885, 999, 1042
LweI GCATC 2 cut(s) 522, 631
MaeI CTAG 2 cut(s) 722, 728
MaeII ACGT 5 cut(s) 16, 99, 345, 546, 659
MaeIII GTNAC 5 cut(s) 9, 467, 866, 902, 928
MalI GATC 2 cut(s) 80, 219
MbiI CCGCTC 1 cut(s) 183
MboI GATC 2 cut(s) 78, 217
MboII GAAGA 4 cut(s) 151, 262, 473, 596
MluCI AATT 3 cut(s) 380, 561, 682
MmeI TCCRAC 1 cut(s) 351
MseI TTAA 2 cut(s) 33, 285
MslI CAYNNNNRTG 1 cut(s) 922
MspA1I CMGCKG 2 cut(s) 453, 835
MspI CCGG 2 cut(s) 309, 663
MvnI CGCG 3 cut(s) 341, 453, 459
MwoI GCNNNNNNNGC 3 cut(s) 450, 835, 947
NcoI CCATGG 2 cut(s) 262, 407
NdeI CATATG 1 cut(s) 1064
NdeII GATC 2 cut(s) 78, 217
NlaIII CATG 4 cut(s) 266, 411, 850, 874
NlaIV GGNNCC 1 cut(s) 524
NmeAIII GCCGAG 1 cut(s) 967
NotI GCGGCCGC 1 cut(s) 454
NspI RCATGY 1 cut(s) 874
NspV TTCGAA 1 cut(s) 351
OliI CACNNNNGTG 1 cut(s) 922
PciI ACATGT 1 cut(s) 870
PciSI GCTCTTC 1 cut(s) 456
PcsI WCGNNNNNNNCGW 1 cut(s) 626
PfeI GAWTC 2 cut(s) 414, 893
Ppu21I YACGTR 1 cut(s) 547
PscI ACATGT 1 cut(s) 870
Psp1406I AACGTT 1 cut(s) 16
PspN4I GGNNCC 1 cut(s) 524
PspPI GGNCC 1 cut(s) 522
PstI CTGCAG 1 cut(s) 1060
PstNI CAGNNNCTG 1 cut(s) 233
RsaI GTAC 2 cut(s) 72, 875
RsaNI GTAC 2 cut(s) 71, 874
RseI CAYNNNNRTG 1 cut(s) 922
SacII CCGCGG 1 cut(s) 454
SapI GCTCTTC 1 cut(s) 456
SaqAI TTAA 2 cut(s) 33, 285
Sau3AI GATC 2 cut(s) 78, 217
Sau96I GGNCC 1 cut(s) 522
ScaI AGTACT 1 cut(s) 72
SfaNI GCATC 2 cut(s) 522, 631
SfcI CTRYAG 2 cut(s) 508, 1056
Sfr303I CCGCGG 1 cut(s) 454
SfuI TTCGAA 1 cut(s) 351
SgrBI CCGCGG 1 cut(s) 454
SinI GGWCC 1 cut(s) 522
SmiMI CAYNNNNRTG 1 cut(s) 922
Sse9I AATT 3 cut(s) 380, 561, 682
SspMI CTAG 2 cut(s) 722, 728
StyI CCWWGG 3 cut(s) 142, 262, 407
TaaI ACNGT 2 cut(s) 934, 1002
TaiI ACGT 5 cut(s) 19, 102, 348, 549, 662
TaqI TCGA 3 cut(s) 138, 351, 483
TasI AATT 3 cut(s) 380, 561, 682
TatI WGTACW 2 cut(s) 70, 873
TauI GCSGC 4 cut(s) 456, 459, 838, 987
TfiI GAWTC 2 cut(s) 414, 893
Tru1I TTAA 2 cut(s) 33, 285
Tru9I TTAA 2 cut(s) 33, 285
TscAI CASTG 3 cut(s) 310, 915, 970
TseI GCWGC 8 cut(s) 107, 441, 735, 842, 862, 898, 987, 1055
TspDTI ATGAA 3 cut(s) 229, 567, 597
TspGWI ACGGA 3 cut(s) 207, 447, 808
TspRI CASTG 3 cut(s) 310, 915, 970
VpaK11BI GGWCC 1 cut(s) 522
XapI RAATTY 1 cut(s) 380
XceI RCATGY 1 cut(s) 874
XmiI GTMKAC 1 cut(s) 626
XspI CTAG 2 cut(s) 722, 728
ZrmI AGTACT 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.