MD16G1278900.v1.1

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014386
Physical Location & Seq
Reverse (-)
37847869 .. 37851824
3956 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1278900.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGGAGGGATTACAACCCTGTAGATATTGGTCTAATGGAGTTTCTCTATTTTTCCAGCCTTTGAGGTTAATACCATTGATCCTTGTGTGTGTTGGAAACGTGGACGCAGCTTTCTACAACCTGACCACCATCCCCTTCGACCAAGGCTATACCCCTCTATTCGGCGATGGCAACCTTGTCCGATCACCCGACGGAAAAGGTGTACGTCTCCTCCTTGATCGCTTCACAGGTTCTGGCTTCATCTCCTCCAATCTTTACAACCATGGTTTCTTCAGCGCTAACATTAAGTTGCCATCCGATTATACTGCCGGTATCTGTGTTGCCTTTTACACATCAAACGCGGACGTATTCGAGAAGAACCACGATGAGTTGGACATAGAATTCTTAGGGAACACAGAAGGGAAGCCATGGAGATTCCAAACCAACCTCTACGGAAATGGCAGCACCAACCGCGGCCGCGAAGAACGTTACCGCCTCTGGTTCGACCCTACGAAAGACTTCCACCGCTACAGCATCCTTTGGACACCCGAAAACACCATATTCTACGTGGATGAAGTCCCAATTAGAGAGGTAGTGCGAAATGAAGAAATGGGCGCTGACTACCCCTCCAAGCCAATGTCCTTGTATACGACTATATGGGATGCAAGCAGTTGGGCCACCTCCGGCGGGCAATACAAGGTGAATTACAAATATGCTCCTTTCGTAGCAGAATTCAAAGACCTAGTCCTAGAGGGCTGCCCCGCCGACCCAATCCAGCAAATCCCTGCCGCTGAAGCGTGCGCGGAACATTACACCCACCTCGCCACCCAAGACTACTCCGTCATCACGCGTCAGCGGCGAGCCGCCATGCGCAGGTTTCGGCAGCGTTACATGTACTACTCATATTGCTACGATTCGTTGCGTTACCCAGTGCCACCATCCGAGTGCGTCACCGTTTCTGCTGAGAAGGCACGCTTCAAGGACACTGGCAGGTTGAAGTTTGGTGGCAGCCACAAACGACAGTCCAAGCGGAGGTCCCGCAACCGAAACCGAGCCGTCCCTAACTCCGACAACGCTTCAGACGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

356

Amino Acids

40.76

Weight (kDa)

8.54

Isoelectric Point (pI)

43.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 48 - 230 4.2e-56 Glycosyl hydrolases family 16
XET_C PF06955 273 - 309 4.1e-13 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014386)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32170
fragaria_vesca FvH4_5g23180 FvH4_5g23180
malus_domestica MD06G1027300.v1.1 MD16G1278900.v1.1
prunus_persica Prupe.5G027800_v2.0.a1
pyrus_communis pycom06g02230 pycom16g24910
rosa_chinensis RchiOBHm_Chr7g0212101
rosa_laevigata RLG00000002935
rosa_multiflora Rmu_sc0002283.1_g000035
rosa_roxburghii Rroxscaffold_3G00247140
rosa_rugosa Rorug07G0131000
rosa_samantha Rh7AG263700 Rh7BG257600 Rh7DG270300
rosa_wichuraiana Rw7G022520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 818
Acc16I TGCGCA 1 cut(s) 851
Acc36I ACCTGC 2 cut(s) 843, 960
AccI GTMKAC 1 cut(s) 626
AccII CGCG 5 cut(s) 341, 453, 459, 782, 829
AclI AACGTT 1 cut(s) 466
AclWI GGATC 1 cut(s) 73
AcoI YGGCCR 1 cut(s) 454
AcsI RAATTY 2 cut(s) 380, 710
AcuI CTGAAG 3 cut(s) 256, 792, 1041
AfaI GTAC 2 cut(s) 204, 875
AfeI AGCGCT 1 cut(s) 277
AfiI CCNNNNNNNGG 4 cut(s) 161, 666, 852, 1011
AflIII ACRYGT 3 cut(s) 827, 870, 1062
AgsI TTSAA 3 cut(s) 715, 958, 976
AjiI CACGTC 1 cut(s) 1063
AluBI AGCT 1 cut(s) 110
AluI AGCT 1 cut(s) 110
Alw26I GTCTC 1 cut(s) 212
AlwI GGATC 1 cut(s) 73
AlwNI CAGNNNCTG 1 cut(s) 233
Aor51HI AGCGCT 1 cut(s) 277
AoxI GGCC 2 cut(s) 454, 654
ApeKI GCWGC 5 cut(s) 107, 441, 735, 862, 987
ApoI RAATTY 2 cut(s) 380, 710
ArsI GACNNNNNNTTYG 2 cut(s) 708, 740
Asp700I GAANNNNTTC 1 cut(s) 497
AspLEI GCGC 4 cut(s) 278, 596, 782, 852
AspS9I GGNCC 2 cut(s) 654, 1014
AsuHPI GGTGA 3 cut(s) 177, 691, 922
AvaII GGWCC 1 cut(s) 1014
BbvI GCAGC 5 cut(s) 119, 453, 722, 874, 999
BccI CCATC 4 cut(s) 137, 161, 301, 925
BceAI ACGGC 1 cut(s) 1019
BcgI CGANNNNNNTGC 2 cut(s) 287, 321
BcoDI GTCTC 1 cut(s) 212
BfaI CTAG 2 cut(s) 722, 728
BfmI CTRYAG 2 cut(s) 19, 508
BfoI RGCGCY 2 cut(s) 279, 597
BfuAI ACCTGC 2 cut(s) 843, 960
Bme18I GGWCC 1 cut(s) 1014
BmgBI CACGTC 1 cut(s) 1063
BmgT120I GGNCC 2 cut(s) 654, 1014
BmiI GGNNCC 1 cut(s) 1016
BmrI ACTGGG 1 cut(s) 902
BmsI GCATC 2 cut(s) 522, 631
BmuI ACTGGG 1 cut(s) 902
BsaAI YACGTR 1 cut(s) 547
BsaJI CCNNGG 4 cut(s) 142, 262, 407, 451
BsaXI ACNNNNNCTCC 4 cut(s) 195, 225, 590, 620
Bsc4I CCNNNNNNNGG 4 cut(s) 161, 666, 852, 1011
Bse118I RCCGGY 1 cut(s) 308
Bse1I ACTGG 2 cut(s) 908, 970
BseDI CCNNGG 4 cut(s) 142, 262, 407, 451
BseGI GGATG 6 cut(s) 129, 293, 513, 556, 646, 917
BseLI CCNNNNNNNGG 4 cut(s) 161, 666, 852, 1011
BseMII CTCAG 1 cut(s) 933
BseNI ACTGG 2 cut(s) 908, 970
BseRI GAGGAG 2 cut(s) 200, 235
BseX3I CGGCCG 1 cut(s) 454
BseXI GCAGC 5 cut(s) 119, 453, 722, 874, 999
Bsh1236I CGCG 5 cut(s) 341, 453, 459, 782, 829
Bsh1285I CGRYCG 1 cut(s) 457
BshFI GGCC 2 cut(s) 456, 656
BsiEI CGRYCG 1 cut(s) 457
BsiSI CCGG 2 cut(s) 309, 663
BslFI GGGAC 3 cut(s) 542, 1000, 1022
BslI CCNNNNNNNGG 4 cut(s) 161, 666, 852, 1011
BsmAI GTCTC 1 cut(s) 212
BsmBI CGTCTC 1 cut(s) 212
BsmFI GGGAC 3 cut(s) 542, 1000, 1022
BsnI GGCC 2 cut(s) 456, 656
Bsp143I GATC 3 cut(s) 78, 182, 217
Bsp19I CCATGG 2 cut(s) 262, 407
BspANI GGCC 2 cut(s) 456, 656
BspCNI CTCAG 1 cut(s) 934
BspFNI CGCG 5 cut(s) 341, 453, 459, 782, 829
BspLI GGNNCC 1 cut(s) 1016
BspMI ACCTGC 2 cut(s) 843, 960
BspPI GGATC 1 cut(s) 73
BsrFI RCCGGY 1 cut(s) 308
BsrI ACTGG 2 cut(s) 908, 970
BssAI RCCGGY 1 cut(s) 308
BssECI CCNNGG 4 cut(s) 142, 262, 407, 451
BssMI GATC 3 cut(s) 78, 182, 217
BssNAI GTATAC 1 cut(s) 627
BssT1I CCWWGG 3 cut(s) 142, 262, 407
Bst1107I GTATAC 1 cut(s) 627
Bst4CI ACNGT 2 cut(s) 934, 1002
BstBAI YACGTR 1 cut(s) 547
BstC8I GCNNGC 5 cut(s) 646, 668, 778, 840, 952
BstDEI CTNAG 2 cut(s) 385, 942
BstDSI CCRYGG 3 cut(s) 262, 407, 451
BstF5I GGATG 6 cut(s) 129, 293, 513, 556, 646, 917
BstFNI CGCG 5 cut(s) 341, 453, 459, 782, 829
BstH2I RGCGCY 2 cut(s) 279, 597
BstHHI GCGC 4 cut(s) 278, 596, 782, 852
BstKTI GATC 3 cut(s) 81, 185, 220
BstMAI GTCTC 1 cut(s) 212
BstMBI GATC 3 cut(s) 78, 182, 217
BstMCI CGRYCG 1 cut(s) 457
BstMWI GCNNNNNNNGC 4 cut(s) 450, 773, 835, 947
BstNSI RCATGY 1 cut(s) 874
BstSFI CTRYAG 2 cut(s) 19, 508
BstUI CGCG 5 cut(s) 341, 453, 459, 782, 829
BstV1I GCAGC 5 cut(s) 119, 453, 722, 874, 999
BstZ17I GTATAC 1 cut(s) 627
BstZI CGGCCG 1 cut(s) 454
BsuRI GGCC 2 cut(s) 456, 656
BtgI CCRYGG 3 cut(s) 262, 407, 451
BtgZI GCGATG 1 cut(s) 180
BtrI CACGTC 1 cut(s) 1063
BtsCI GGATG 6 cut(s) 129, 293, 513, 556, 646, 917
BtsIMutI CAGTG 2 cut(s) 915, 963
BveI ACCTGC 2 cut(s) 843, 960
Cac8I GCNNGC 5 cut(s) 646, 668, 778, 840, 952
CaiI CAGNNNCTG 1 cut(s) 233
CciNI GCGGCCGC 1 cut(s) 454
CfoI GCGC 4 cut(s) 278, 596, 782, 852
Cfr10I RCCGGY 1 cut(s) 308
Cfr13I GGNCC 2 cut(s) 654, 1014
Cfr42I CCGCGG 1 cut(s) 454
CseI GACGC 3 cut(s) 113, 818, 916
Csp6I GTAC 2 cut(s) 203, 874
CviAII CATG 4 cut(s) 263, 408, 847, 871
CviQI GTAC 2 cut(s) 203, 874
DdeI CTNAG 2 cut(s) 385, 942
DpnI GATC 3 cut(s) 80, 184, 219
DpnII GATC 3 cut(s) 78, 182, 217
DrdI GACNNNNNNGTC 1 cut(s) 818
DseDI GACNNNNNNGTC 1 cut(s) 818
EaeI YGGCCR 1 cut(s) 454
EagI CGGCCG 1 cut(s) 454
EclXI CGGCCG 1 cut(s) 454
Eco130I CCWWGG 3 cut(s) 142, 262, 407
Eco47I GGWCC 1 cut(s) 1014
Eco47III AGCGCT 1 cut(s) 277
Eco52I CGGCCG 1 cut(s) 454
Eco57I CTGAAG 3 cut(s) 256, 792, 1041
EcoO109I RGGNCCY 1 cut(s) 1014
EcoRI GAATTC 2 cut(s) 380, 710
EcoT14I CCWWGG 3 cut(s) 142, 262, 407
ErhI CCWWGG 3 cut(s) 142, 262, 407
Esp3I CGTCTC 1 cut(s) 212
FaeI CATG 4 cut(s) 266, 411, 850, 874
FalI AAGNNNNNCTT 2 cut(s) 938, 970
FaqI GGGAC 3 cut(s) 542, 1000, 1022
FatI CATG 4 cut(s) 262, 407, 846, 870
FauI CCCGC 3 cut(s) 659, 748, 1025
FblI GTMKAC 1 cut(s) 626
FokI GGATG 6 cut(s) 116, 280, 500, 563, 653, 904
FspBI CTAG 2 cut(s) 722, 728
FspI TGCGCA 1 cut(s) 851
GlaI GCGC 4 cut(s) 277, 595, 781, 851
HaeII RGCGCY 2 cut(s) 279, 597
HaeIII GGCC 2 cut(s) 456, 656
HapII CCGG 2 cut(s) 309, 663
HgaI GACGC 3 cut(s) 113, 818, 916
HhaI GCGC 4 cut(s) 278, 596, 782, 852
Hin1II CATG 4 cut(s) 266, 411, 850, 874
Hin6I GCGC 4 cut(s) 276, 594, 780, 850
HinP1I GCGC 4 cut(s) 276, 594, 780, 850
HinfI GANTC 2 cut(s) 414, 893
HpaII CCGG 2 cut(s) 309, 663
HphI GGTGA 3 cut(s) 177, 691, 922
Hpy166II GTNNAC 3 cut(s) 103, 203, 627
Hpy188I TCNGA 5 cut(s) 182, 298, 922, 1048, 1060
Hpy188III TCNNGA 1 cut(s) 352
Hpy8I GTNNAC 3 cut(s) 103, 203, 627
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 3 cut(s) 145, 392, 940
HpyCH4III ACNGT 2 cut(s) 934, 1002
HpyCH4IV ACGT 6 cut(s) 99, 205, 345, 466, 546, 1062
HpyCH4V TGCA 1 cut(s) 644
HpyF10VI GCNNNNNNNGC 4 cut(s) 450, 773, 835, 947
HpyF3I CTNAG 2 cut(s) 385, 942
HpySE526I ACGT 6 cut(s) 99, 205, 345, 466, 546, 1062
Hsp92II CATG 4 cut(s) 266, 411, 850, 874
HspAI GCGC 4 cut(s) 276, 594, 780, 850
KspI CCGCGG 1 cut(s) 454
Kzo9I GATC 3 cut(s) 78, 182, 217
LmnI GCTCC 1 cut(s) 700
Lsp1109I GCAGC 5 cut(s) 119, 453, 722, 874, 999
LweI GCATC 2 cut(s) 522, 631
MaeI CTAG 2 cut(s) 722, 728
MaeII ACGT 6 cut(s) 99, 205, 345, 466, 546, 1062
MaeIII GTNAC 4 cut(s) 467, 866, 902, 928
MalI GATC 3 cut(s) 80, 184, 219
MboI GATC 3 cut(s) 78, 182, 217
MboII GAAGA 4 cut(s) 262, 367, 473, 596
MluCI AATT 4 cut(s) 380, 561, 682, 710
MluI ACGCGT 1 cut(s) 827
MmeI TCCRAC 2 cut(s) 73, 351
MroXI GAANNNNTTC 1 cut(s) 497
MseI TTAA 2 cut(s) 68, 285
MslI CAYNNNNRTG 1 cut(s) 922
MspA1I CMGCKG 3 cut(s) 453, 770, 835
MspI CCGG 2 cut(s) 309, 663
MvnI CGCG 5 cut(s) 341, 453, 459, 782, 829
MwoI GCNNNNNNNGC 4 cut(s) 450, 773, 835, 947
NcoI CCATGG 2 cut(s) 262, 407
NdeII GATC 3 cut(s) 78, 182, 217
NlaIII CATG 4 cut(s) 266, 411, 850, 874
NlaIV GGNNCC 1 cut(s) 1016
NmuCI GTSAC 1 cut(s) 928
NotI GCGGCCGC 1 cut(s) 454
NsbI TGCGCA 1 cut(s) 851
NspI RCATGY 1 cut(s) 874
PciI ACATGT 1 cut(s) 870
PcsI WCGNNNNNNNCGW 1 cut(s) 1059
PdmI GAANNNNTTC 1 cut(s) 497
PfeI GAWTC 2 cut(s) 414, 893
PflFI GACNNNGTC 1 cut(s) 722
Ppu21I YACGTR 1 cut(s) 547
PpuMI RGGWCCY 1 cut(s) 1014
PscI ACATGT 1 cut(s) 870
Psp1406I AACGTT 1 cut(s) 466
Psp5II RGGWCCY 1 cut(s) 1014
PspN4I GGNNCC 1 cut(s) 1016
PspPI GGNCC 2 cut(s) 654, 1014
PspPPI RGGWCCY 1 cut(s) 1014
PstNI CAGNNNCTG 1 cut(s) 233
PsyI GACNNNGTC 1 cut(s) 722
RsaI GTAC 2 cut(s) 204, 875
RsaNI GTAC 2 cut(s) 203, 874
RseI CAYNNNNRTG 1 cut(s) 922
SacII CCGCGG 1 cut(s) 454
SaqAI TTAA 2 cut(s) 68, 285
Sau3AI GATC 3 cut(s) 78, 182, 217
Sau96I GGNCC 2 cut(s) 654, 1014
SfaNI GCATC 2 cut(s) 522, 631
SfcI CTRYAG 2 cut(s) 19, 508
Sfr303I CCGCGG 1 cut(s) 454
SgrBI CCGCGG 1 cut(s) 454
SinI GGWCC 1 cut(s) 1014
SmiMI CAYNNNNRTG 1 cut(s) 922
Sse9I AATT 4 cut(s) 380, 561, 682, 710
SspMI CTAG 2 cut(s) 722, 728
StyI CCWWGG 3 cut(s) 142, 262, 407
TaaI ACNGT 2 cut(s) 934, 1002
TaiI ACGT 6 cut(s) 102, 208, 348, 469, 549, 1065
TaqI TCGA 3 cut(s) 138, 351, 483
TasI AATT 4 cut(s) 380, 561, 682, 710
TatI WGTACW 1 cut(s) 873
TauI GCSGC 5 cut(s) 456, 459, 770, 838, 845
TfiI GAWTC 2 cut(s) 414, 893
Tru1I TTAA 2 cut(s) 68, 285
Tru9I TTAA 2 cut(s) 68, 285
TscAI CASTG 2 cut(s) 915, 970
TseFI GTSAC 1 cut(s) 928
TseI GCWGC 5 cut(s) 107, 441, 735, 862, 987
Tsp45I GTSAC 1 cut(s) 928
TspDTI ATGAA 3 cut(s) 229, 567, 597
TspGWI ACGGA 3 cut(s) 207, 447, 808
TspRI CASTG 2 cut(s) 915, 970
Tth111I GACNNNGTC 1 cut(s) 722
VpaK11BI GGWCC 1 cut(s) 1014
XapI RAATTY 2 cut(s) 380, 710
XceI RCATGY 1 cut(s) 874
XcmI CCANNNNNNNNNTGG 1 cut(s) 544
XmiI GTMKAC 1 cut(s) 626
XmnI GAANNNNTTC 1 cut(s) 497
XspI CTAG 2 cut(s) 722, 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.